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Thomas E Rams, Jacqueline D Sautter, Chinhua Y Hsiao, Arie J van Winkelhoff
The accuracy of a phenotypic scheme to recognize periodontal Prevotella intermedia/nigrescens group clinical isolates on primary isolation culture plates was assessed with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 84 fresh subgingival isolates from 23 chronic periodontitis patients were presumptively recognized on anaerobically-incubated enriched Brucella blood agar primary isolation plates as P. intermedia/nigrescens based on their dark-pigmented colony morphology, brick-red autofluorescence under long-wave ultraviolet light, and a negative fluorescence test for lactose production...
June 15, 2018: Anaerobe
Paweł Kubasiewicz-Ross, Jakub Hadzik, Marzena Dominiak
BACKGROUND: Zirconium - a bioinert metal - in comparison with titanium implants, offers a variety of potential advantages for use in the esthetic area of dentistry due to its tooth-like color. Zirconium dental implants are considered to be an alternative method of treatment to conventional titanium dental implants for patients with a thin gingival biotype. OBJECTIVES: This study was designed to study the bone tissue response to new zirconia implants with modified surfaces in comparison with commercially available titanium dental implants and commercially available zirconia implants...
June 18, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Mushtaq Ahmad, Khalid P Akhtar
The cotton whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a serious pest of several summer crops in hot and dry climates. Its field populations (Asia II-1 biotype) were assessed for their susceptibility to diverse pesticides by using leaf-dip bioassay. There was no or a very low resistance to amitraz, hexythiazox, and pyridaben during 1992-2015. B. tabaci also exhibited no resistance to endosulfan during 1992-1997 and a very low resistance during 1998-2010, which then rose to a low level during 2011-2015...
April 18, 2018: Journal of Economic Entomology
A C M Veloo, H Jean-Pierre, U S Justesen, T Morris, E Urban, I Wybo, M Kostrzewa, A W Friedrich, On Behalf Of The Enria Workgroup
This data in brief article presents the data obtained during the validation of the optimized Biotyper Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) database. The validation was performed by the different expertise laboratories, collaborating within the European Network for the Rapid Identification of Anaerobes (ENRIA) project, using 6309 human clinical anaerobic bacterial strains. Different databases were compared with each other; the db 5989 database (V5 database); the V5 database complimented with Main Spectral Profiles (MSPs) of ENRIA strains added to the next update of the database; and the V5 database complimented with the MSPs of all anaerobic clinical isolates collected within the ENRIA project...
June 2018: Data in Brief
Rodrigo Soares Ramos, Lalit Kumar, Farzin Shabani, Marcelo Coutinho Picanço
The whitefly, Bemisia tabaci, is a major threat to tomato Solanum lycopersicum and ranks as one of the world's 100 most invasive pests. This is the first study of B. tabaci (Biotype B and Q) global distribution, focusing on risk levels of this invasive pest, in areas projected to be suitable for open field S. lycopersicum cultivation under climate change. This study aims to identify levels of risk of invasive B. tabaci for areas of suitability for open field S. lycopersicum cultivation for the present, 2050 and 2070 using MaxEnt and the Global Climate Model, HadGEM2_ES under RCP45...
2018: PloS One
Laurent Dortet, Didier Tandé, Dominique de Briel, Sandrine Bernabeu, Camille Lasserre, Guillaume Gregorowicz, Agnès B Jousset, Thierry Naas
Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here, we have evaluated three MALDI-TOF-based techniques to detect carbapenemase-producing Enterobacteriaceae (CPE) from cultured colonies. Methods: The performance of three MALDI-TOF-based techniques, including the commercialized MBT STAR®-Carba IVD Kit (Bruker Daltonics) and two in-house protocols performed on the Microflex LT Biotyper (Bruker Daltonics) and the VITEK® MS Plus (bioMérieux), were compared with those of the RAPIDEC® CARBA NP (bioMérieux)...
June 11, 2018: Journal of Antimicrobial Chemotherapy
Berhoz K Tahmasebi, Ricardo Alcántara-de la Cruz, Esteban Alcántara, Joel Torra, José A Domínguez-Valenzuela, Hugo E Cruz-Hipólito, Antonia M Rojano-Delgado, Rafael De Prado
The use of herbicides with different modes of action is the primary strategy used to control weeds possessing resistance to a single mechanism of action (MOA). However, this practice can lead to selection for generalist resistance mechanisms and may cause resistance to all MOAs. In this research, we characterized the resistance to diquat/paraquat (bipyridiliums) in an Epilobium ciliatum biotype (R1) collected in an olive orchard from Chile, where alternatives herbicides (2,4-D, glyphosate, glufosinate, flazasulfuron and pyraflufen-ethyl) with different MOAs were used, but they have also showed failure in controlling this species...
2018: Frontiers in Plant Science
Geoffrey B Severin, Miriam S Ramliden, Lisa A Hawver, Kun Wang, Macy E Pell, Ann-Katrin Kieninger, Atul Khataokar, Brendan J O'Hara, Lara V Behrmann, Matthew B Neiditch, Christoph Benning, Christopher M Waters, Wai-Leung Ng
Sensing and responding to environmental changes is essential for bacteria to adapt and thrive, and nucleotide-derived second messengers are central signaling systems in this process. The most recently identified bacterial cyclic dinucleotide second messenger, 3', 3'-cyclic GMP-AMP (cGAMP), was first discovered in the El Tor biotype of Vibrio cholerae The cGAMP synthase, DncV, is encoded on the VSP-1 pathogenicity island, which is found in all El Tor isolates that are responsible for the current seventh pandemic of cholera but not in the classical biotype...
June 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
Snorre Gulla, Andrew C Barnes, Timothy J Welch, Jesús L Romalde, David Ryder, Michael J Ormsby, Jeremy Carson, Karin Lagesen, David W Verner-Jeffreys, Robert L Davies, Duncan J Colquhoun
A Multi-Locus Variable number of tandem repeat Analysis (MLVA) assay was developed for epizootiological study of the internationally significant fish pathogen Yersinia ruckeri , which causes yersiniosis in salmonids. The assay involves amplification of ten Variable Number of Tandem Repeat (VNTR) loci in two five-plex PCR reactions, followed by capillary electrophoresis. A collection of 484 Y. ruckeri isolates, originating from various biological sources and collected from four continents over seven decades, was analysed...
June 8, 2018: Applied and Environmental Microbiology
Nurver Ulger Toprak, Veloo Alida C M, Edit Urban, Ingrid Wybo, Justesen Ulrik S, Helene Jean-Pierre, Trefor Morris, Oncu Akgul, Guven Kulekci, Guner Soyletir, Elisabeth Nagy
Prevotella species, members of the human microbiota, can cause opportunistic infections. Rapid and accurate identification of Prevotella isolates plays a critical role in successful treatment, especially since the antibiotic susceptibility profile differs between species. Studies, mostly carried out using the Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Biotyper system, showed that MALDI-TOF MS is an accurate, rapid and satisfactory method for the identification of clinically important anaerobes...
May 30, 2018: Anaerobe
Dexin Wang, Fanggui Sun, Zheng Li, Yutong Hu, Rongming Xu
We present the case of a 91-year-old woman who presented with a 2‑day history of progressive pain and immobility of the right shoulder joint after fever of unknown etiology. Aeromonas sobria was isolated from a culture of purulent synovial fluid. The clinical condition gradually improved with the application of appropriate antibiotics and no surgical intervention was necessary. This report indicates that acute septic arthritis may result from Aeromonas veronii biotype sobria infections in healthy people...
June 5, 2018: Der Orthopäde
Angus Angermeyer, Moon Moon Das, Durg Vijai Singh, Kimberley D Seed
The Vibrio cholerae biotype "El Tor" is responsible for all of the current epidemic and endemic cholera outbreaks worldwide. These outbreaks are clonal, and it is hypothesized that they originate from the coastal areas near the Bay of Bengal, where the lytic bacteriophage ICP1 (International Centre for Diarrhoeal Disease Research, Bangladesh cholera phage 1) specifically preys upon these pathogenic outbreak strains. ICP1 has also been the dominant bacteriophage found in cholera patient stools since 2001...
June 1, 2018: Viruses
Khadigeh Sirghani, Tayebeh Zeinali, Abdollah Jamshidi
Poultry meat is one of the most important sources of infection of Yersinia spp. for humans. The aim of the present study was to evaluate the incidence of Yersinia enterocolitica in chicken meat by using culture method on selective medium and confirmation by PCR assay. Also, biochemical methods were used for biotyping. A total of 100 chicken thigh meat samples were collected randomly from retail outlets in Mashhad, Iran. Samples were enriched in Peptone-Sorbitol-Bile (PSB) broth and then cultured on Cefsulodin-Irgasan-Novobiocin (CIN) agar containing antibiotics supplement...
2018: Journal of Pathogens
Arumugam Murugananthan, Sudarvili Shanthalingam, Sai Arun Batra, Sitara Alahan, Subramaniam Srikumaran
Bibersteinia trehalosi and Mannheimia haemolytica , originally classified as Pasteurella haemolytica biotype T and biotype A, respectively, under Genus Pasteurella has now been placed under two different Genera, Bibersteinia and Mannheimia , based on DNA-DNA hybridization and 16S RNA studies. While M. haemolytica has been the predominant pathogen of pneumonia in ruminants, B. trehalosi is emerging as an important pathogen of ruminant pneumonia. Leukotoxin is the critical virulence factor of these two pathogens...
May 30, 2018: Toxins
Lukas Hleba, Ivana Charousova, Miroslava Cisarova, Anton Kovacik, Jan Kormanec, Juraj Medo, Matej Bozik, Sona Javorekova
The main objective of this study was using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for assembling of DSM (German Collection of Microorganisms) Streptomyces spectral database and identification of wild Streptomyces cultures, which were clustered by MALDI-TOF Biotyper OC software as well as for teracycline detection by observing of obtained spectra using flexAnalysis software. Production of tetracycline was confirmed by thin-layer chromatography. Presence of tetracycline mass spectrum was verified by several tetracycline producers (Streptomyces aureofaciens LMG 5968, S...
May 29, 2018: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
Raedeen Russell, Hongxia Wang, Jorge A Benitez, Anisia J Silva
Hypervirulent atypical El Tor biotype Vibrio cholerae O1 isolates harbour mutations in the DNA-binding domain of the nucleoid-associated protein H-NS and the receiver domain of the response regulator VieA. Here, we provide two examples in which inactivation of H-NS in El Tor biotype vibrios unmasks hidden regulatory connections. First, deletion of the helix-turn-helix domain of VieA in an hns mutant background diminished biofilm formation and exopolysaccharide gene expression, a function that phenotypically opposes its phosphodiesterase activity...
May 29, 2018: Microbiology
Jan Hrček, Benjamin J Parker, Ailsa H C McLean, Jean-Christophe Simon, Ciara M Mann, H Charles J Godfray
Microbial symbionts commonly protect their hosts from natural enemies, but it is unclear how protective symbionts influence the evolution of host immunity to pathogens. One possibility is that 'extrinsic' protection provided by symbionts allows hosts to reduce investment in 'intrinsic' immunological resistance mechanisms. We tested this idea using pea aphids (Acyrthosiphon pisum) and their facultative bacterial symbionts that increase host resistance to the fungal pathogen Pandora neoaphidis. The pea aphid taxon is composed of multiple host plant associated populations called biotypes, which harbour characteristic communities of symbionts...
May 28, 2018: Evolution; International Journal of Organic Evolution
Elpiniki Vandera, Georgia Tsirka, Athanasia Kakouri, Anna-Irini Koukkou, John Samelis
Enterococci are naturally selected for growth in thermized ewes'/goats' milk mixtures used for traditional cooked hard cheese processing in Greece. A culture-independent PCR-based approach was applied to detect the presence of enterocin-encoding genes in naturally culture-enriched thermized milk (TM). Portions of TM (63 °C, 30 s) collected from a commercial cheese plant before addition of starters were fermented at 37 °C for 48 h to facilitate growth of indigenous enterococci. The multiple enterocin-producing (m-Ent+) Enterococcus faecium KE82 and the nisin A-producing Lactococcus lactis subsp...
May 21, 2018: International Journal of Food Microbiology
Jian Zhang, Xiaojuan Zhu, Ruitao Xu, Qiang Gao, Depei Wang, Ying Zhang
Histamine (HIS) producers in fermented wines are generally believed to be lactic acid bacteria (LAB), and other microorganisms have received little or no attention. In this work, HIS-producing bacteria were isolated from Qu fermentation starter for Chinese rice wine brewing by decarboxylase medium, and their identity was confirmed by RP-HPLC and PCR. Surprisingly, the histidine decarboxylase gene (hdc) was present in only 2 out of 26 isolates. All 26 isolates were genotyped using the randomly amplified polymorphic DNA (RAPD)-PCR assay, which revealed the presence of 21 biotypes...
May 17, 2018: International Journal of Food Microbiology
Douglas F Dluzen, Nicole Noren Hooten, Supriyo De, William H Wood, Yongqing Zhang, Kevin G Becker, Alan B Zonderman, Toshiko Tanaka, Luigi Ferrucci, Michele K Evans
Circulating extracellular RNAs (exRNAs) are potential biomarkers of disease. We thus hypothesized that age-related changes in exRNAs can identify age-related processes. We profiled both large and small RNAs in human serum to investigate changes associated with normal aging. exRNA was sequenced in 13 young (30-32 years) and 10 old (80-85 years) African American women to identify all RNA transcripts present in serum. We identified age-related differences in several RNA biotypes, including mitochondrial transfer RNAs, mitochondrial ribosomal RNA, and unprocessed pseudogenes...
May 24, 2018: Aging Cell
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