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https://www.readbyqxmd.com/read/28730523/antibody-dependent-enhancement-of-serotype-ii-feline-enteric-coronavirus-infection-in-primary-feline-monocytes
#1
Tomomi Takano, Mamiko Nakaguchi, Tomoyoshi Doki, Tsutomu Hohdatsu
Feline coronavirus (FCoV) has been classified into two biotypes: avirulent feline coronavirus (feline enteric coronavirus: FECV) and virulent feline coronavirus (feline infectious peritonitis virus: FIPV). In FIPV infection, antibody-dependent enhancement (ADE) has been reported and was shown to be associated with severe clinical disease. On the other hand, the potential role of ADE in FECV infection has not been examined. In this study, using laboratory strains of serotype II FIPV WSU 79-1146 (FIPV 79-1146) and serotype II FECV WSU 79-1683 (FECV 79-1683), we investigated the relationship between ADE and induction of inflammatory cytokines, which are pathogenesis-related factors, for each strain...
July 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28727461/risk-indicators-and-prevention-of-implant-soft-tissue-complications-interproximal-papillae-loss-and-midfacial-implant-mucosal-recessions
#2
James Mailoa, Richard J Miron, Hom-Lay Wang
Achieving a long-term esthetic implant restoration in the maxillary anterior area is sometimes unpredictable. This article reviews several risk indicators of implant mucosal recession and loss of interproximal papillae, and how to prevent these risks. Results obtained from the literature suggest that the following factors are strongly related to implant soft-tissue complications: buccally positioned implant placement, a thin gingival biotype, lack of an adequate amount of keratinized mucosa, implant placement after ridge augmentation procedures, an increased distance of tooth bone peak to the contact point, and an inadequate horizontal implant-tooth distance...
July 2017: Compendium of Continuing Education in Dentistry
https://www.readbyqxmd.com/read/28713658/morphological-variation-of-aphidius-ervi-haliday-hymenoptera-braconidae-associated-with-different-aphid-hosts
#3
Cinthya M Villegas, Vladimir Žikić, Saša S Stanković, Sebastián A Ortiz-Martínez, Ainara Peñalver-Cruz, Blas Lavandero
BACKGROUND: Parasitoids are frequently used in biological control due to the fact that they are considered host specific and highly efficient at attacking their hosts. As they spend a significant part of their life cycle within their hosts, feeding habits and life history of their host can promote specialization via host-race formation (sequential radiation). The specialized host races from different hosts can vary morphologically, behaviorally and genetically. However, these variations are sometimes inconspicuous and require more powerful tools in order to detect variation such as geometric morphometrics analysis...
2017: PeerJ
https://www.readbyqxmd.com/read/28713359/exploring-the-diversity-of-field-strains-of-brucella-abortus-biovar-3-isolated-in-west-africa
#4
Moussa Sanogo, David Fretin, Eric Thys, Claude Saegerman
Brucellosis is one of the most widespread bacterial zoonotic diseases in the world, affecting both humans and domestic and wild animals. Identification and biotyping of field strains of Brucella are of key importance for a better knowledge of the epidemiology of brucellosis, for identifying appropriate antigens, for managing disease outbreaks and for setting up efficient preventive and control programmes. Such data are required both at national and regional level to assess potential threats for public health...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28712383/retrospective-genomic-analysis-of-vibrio-cholerae-o1-el-tor-strains-from-different-places-in-india-reveals-the-presence-of-ctxb-7-allele-found-in-haitian-isolates
#5
R DE, T Ramamurthy, B L Sarkar, A K Mukhopadhyay, G P Pazhani, S Sarkar, S Dutta, G B Nair
A total of 45 strains of Vibrio cholerae O1 isolated from 10 different places in India where they were associated with cases of cholera between the years 2007 and 2008 were examined by molecular methods. With the help of phenotypic and genotypic tests the strains were confirmed to be O1 El Tor biotype strains with classical ctxB gene. Polymerase chain reaction (PCR) analysis by double - mismatch amplification mutation assay PCR showed 16 of these strains carried the ctxB-7 allele reported in Haitian strains...
August 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28708321/dynamics-of-culturable-mesophilic-bacterial-communities-of-three-fresh-herbs-and-their-production-environment
#6
Maria-Theresia Gekenidis, Diane Gossin, Mathias Schmelcher, Ulrich Schöner, Mitja N P Remus-Emsermann, David Drissner
AIM: Investigate dynamics of culturable mesophilic bacteria and selected food contaminating bacteria from three herbs and their production environment. METHODS AND RESULTS: Marjoram, basil, and thyme were investigated during one growing season by sampling plants, organic fertilizers, soil, irrigation water, and marketed products. Mesophilic bacteria and selected food contaminating bacteria (Escherichia coli, Enterococcus spp., Bacillus cereus group) were cultured and identified by MALDI biotyping...
July 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28707702/secondary-immune-response-of-rainbow-trout-following-repeated-immersion-vaccination
#7
R M Jaafar, A Al-Jubury, J K Chettri, I Dalsgaard, P W Kania, K Buchmann
Teleosts are able to raise a protective immune response, comprising both innate and adaptive elements, against various pathogens. This is the basis for a widespread use of vaccines, administered as injection or immersion, in the aquaculture industry. It has been described that repeated injection vaccination of fish raises a secondary immune response, consisting of rapid, accelerated and increased antibody reaction. This study reports how rainbow trout responds to repeated immersion vaccination against yersiniosis (ERM) caused by the bacterial pathogen Yersinia ruckeri...
July 14, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28706514/evaluation-of-the-bruker-biotyper-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-system-for-identification-of-aspergillus-species-directly-from-growth-on-solid-agar-media
#8
Ying Li, He Wang, Yu-Pei Zhao, Ying-Chun Xu, Po-Ren Hsueh
We evaluated the accuracy of the Bruker Biotyper matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) system at identifying clinical isolates of Aspergillus species that were grown on agar media. A total of 381 non-duplicate Aspergillus isolates representing 21 different Aspergillus species identified by molecular analysis were included in this study. The Bruker Biotyper MALDI-TOF MS system was able to identify 30.2% (115/381) of the isolates to the species level (score values of ≥2...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28705667/clinical-characteristics-of-bacteraemia-caused-by-burkholderia-cepacia-complex-species-and-antimicrobial-susceptibility-of-the-isolates-in-a-medical-centre-in-taiwan
#9
Ying-Chun Chien, Chun-Hsing Liao, Wang-Huei Sheng, Jung-Yien Chien, Yu-Tsung Huang, Chong-Jen Yu, Po-Ren Hsueh
This study investigated the clinical characteristics and outcomes of bacteraemia due to Burkholderia cepacia complex (BCC) species among 54 patients without cystic fibrosis from January 2013 to February 2015. BCC isolates were identified to the species level by the Bruker Biotyper MALDI-TOF MS system and by sequencing analysis of the 16S rRNA and recA genes. Antimicrobial susceptibilities of the isolates were determined by the agar dilution method. Sequencing of the recA gene in the 54 blood isolates revealed 37 (68...
July 10, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28704728/development-of-a-novel-multiplexed-qpcr-and-pyrosequencing-method-for-the-detection-of-human-pathogenic-yersiniae
#10
M C Thomas, T W Janzen, G Huscyzynsky, A Mathews, K K Amoako
The purpose of this study was to develop a novel and robust molecular assay for the detection of human pathogenic yersiniae (i.e. Yersinia enterocolitica, Y. pseudotuberculosis and Y. pestis) in complex food samples. The assay combines multiplexed real-time PCR (qPCR) and Pyrosequencing for detecting and differentiating human pathogenic yersiniae with high confidence through sequence based confirmation. The assay demonstrated 100% specificity and inclusivity when tested against a panel of 14 Y. enterocolitica, 22 Y...
June 20, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28702931/autophagy-induced-by-bovine-viral-diarrhea-virus-infection-counteracts-apoptosis-and-innate-immune-activation
#11
Yulong Zhou, Yachao Ren, Yanlong Cong, Yu Mu, Renfu Yin, Zhuang Ding
Bovine viral diarrhea virus (BVDV) is an important pathogen of cattle that plays a complex role in disease. There are two biotypes of BVDV: non-cytopathic (NCP) and cytopathic (CP). One strategy that has been used to treat or prevent virus-associated diseases is the modulation of autophagy, which is used by the innate immune system to defend against viral infection; however, at present, the interplay between autophagy and BVDV remains unclear. Madin-Darby bovine kidney cells stably expressing microtubule-associated protein 1 light chain 3B (LC3B) with green fluorescent protein (GFP) (GFP-LC3-MDBK cells) and autophagy-deficient MDBKs (shBCN1-MDBK cells) were constructed...
July 12, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28702460/seroprevalence-and-distribution-of-contagious-bovine-pleuropneumonia-in-ethiopia-update-and-critical-analysis-of-20-years-1996-2016-reports
#12
REVIEW
Nejash Abdela, Nesradin Yune
Contagious bovine pleuropneumonia (CBPP), infectious and highly contagious diseases of cattle in Africa, is the only bacterial disease in the OIE list A diseases. This severe respiratory disease of cattle is the second most important transboundary animal disease in Africa after rinderpest. CBPP is caused by Mycoplasma mycoides subsp. mycoides SC (small colony bovine biotype) and it is endemic disease in many African countries including Ethiopia. This paper systematically reviews prevalence report at herd and individual animal level for the last 20 years (1996-2016) with main aim of making comprehensive document regarding the seroprevalence and distribution of CBPP in Ethiopia...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28698734/methods-of-biotyping-of-streptococcus-mutans-species-with-the-routine-test-as-a-prognostic-value-in-early-childhood-caries
#13
Wirginia Krzyściak, Dorota Kościelniak, Monika Papież, Anna Jurczak, Palina Vyhouskaya
PURPOSE: In order to investigate the suitability of Streptococcus mutans species biotyping by measuring the activity of selected enzymes from a commercial test, criteria were established for biotyping clinical strains from children with dental caries. In addition, the relationships between the selected biotypes, sensitivity to commonly used antibiotics, and early childhood caries were determined. METHODS: A total of 142 S. mutans isolates from dental plaque of children with caries were divided into different biotypes...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28694450/modelling-west-nile-virus-transmission-risk-in-europe-effect-of-temperature-and-mosquito-biotypes-on-the-basic-reproduction-number
#14
Chantal B F Vogels, Nienke Hartemink, Constantianus J M Koenraadt
West Nile virus (WNV) is a mosquito-borne flavivirus which has caused repeated outbreaks in humans in southern and central Europe, but thus far not in northern Europe. The main mosquito vector for WNV, Culex pipiens, consists of two behaviourally distinct biotypes, pipiens and molestus, which can form hybrids. Differences between biotypes, such as vector competence and host preference, could be important in determining the risk of WNV outbreaks. Risks for WNV establishment can be modelled with basic reproduction number (R 0) models...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28676807/mechanism-of-resistance-in-mungbean-vigna-radiata-l-r-wilczek-var-radiata-to-bruchids-callosobruchus-spp-coleoptera-bruchidae
#15
REVIEW
Abdul R War, Surya Murugesan, Venkata N Boddepalli, Ramasamy Srinivasan, Ramakrishnan M Nair
Mungbean [Vigna radiata (L.) R. Wilczek var. radiata] is an important pulse crop in Asia, and is consumed as dry seeds and as bean sprouts. It is an excellent source of digestible protein. Bruchids [Callosobruchus chinensis (L.) and Callosobruchus maculatus (F.)] are the important pests of mungbean and cause damage in the field and in storage. Bruchid infestation reduces the nutritional and market value of the grain and renders seeds unfit for human consumption, agricultural and commercial uses. These pests are controlled mainly by fumigation with highly toxic chemicals such as carbon disulfide, phosphene, and methyl bromide, or by dusting with several other insecticides, which leave residues on the grain, thus, threatening food safety...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28674316/vibrio-cholerae-o1-ogawa-strains-carrying-the-ctxb7-allele-caused-large-cholera-outbreak-during-2014-in-the-tribal-areas-of-odisha-india
#16
Bibhuti Bhusan Pal, Hemant Kumar Khuntia, Smruti Ranjan Nayak, Anima Mohanty, Bhagyalaxmi Biswal
The large outbreak of cholera reported during July to Sept, 2014 in Narla block of Kalahandi district was investigated to find out the causative organism. The rectal swabs collected from diarrhoea patients and environmental water samples were sub-cultured following standard techniques. The causative organism was V.cholerae O1 Ogawa biotype El Tor while DMAMA-PCR assay confirmed that all strains were ctxB7 of Haitian variants V.cholerae O1. The water samples were negative for V. cholerae .The V.cholerae O1 strains were sensitive to tetracycline, ciprofloxacin, norfloxacin, ofloxacin, doxycycline, azithromycin,; but were resistant to erythromycin, gentamicin, chloramphenicol, furazolidone, neomycine, cotrimoxazole, nalidixic acid and ampicilin...
July 1, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28667741/coronal-advanced-flap-in-combination-with-a-connective-tissue-graft-is-the-thickness-of-the-flap-a-predictor-for-root-coverage-a-prospective-clinical-study
#17
Teresa Garcés, Josep Maria Carbonell, Lluis Vallcorba, Antonio Santos, Cristina Valles, Jose Nart
AIM: Evaluate if there is any relationship between the flap thickness and the presence of complete root coverage when performing coronally advanced flaps in combination with a connective tissue graft. MATERIAL AND METHODS: Prospective clinical study, in which multiple Miller class I and II recessions were treated with a coronally advanced flap and a connective tissue graft standardized at 1mm of thickness. Individual stents permitted repeated mesurements of conventional periodontal parameters at the same point...
July 1, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28666749/prevalences-of-antimicrobial-resistance-and-the-cfia-resistance-gene-in-danish-bacteroides-fragilis-group-isolates-since-1973
#18
Simon Andreas Ferløv-Schwensen, Thomas Vognbjerg Sydenham, Kia Cirkeline Hansen, Silje Vermedal Hoegh, Ulrik Stenz Justesen
OBJECTIVES: The purpose of this study was to determine the prevalences of resistance and the cfiA carbapenemase-producing gene in historical Bacteroides fragilis group isolates. METHODS: Danish clinical B. fragilis group isolates (n=444) spanning from 1973 to 2015 were identified with Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) on the Biotyper platform. Antimicrobial resistance was determined using a disk diffusion screening method and commercial antibiotic gradient strips...
June 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28656386/performance-of-microbial-identification-by-maldi-tof-ms-and-susceptibility-testing-by-vitek-2-from-positive-blood-cultures-after-minimal-incubation-on-solid-media
#19
S L Mitchell, K Alby
Bloodstream infections (BSIs) are a leading cause of patient morbidity and mortality. Rapid identification of organisms from BSIs is critical for initiating targeted antimicrobial therapy. Although many methods exist for rapid identification, they do not provide detailed or definitive susceptibility information. We assessed the utility of both the VITEK MS and Bruker Biotyper MALDI-TOF mass spectrometers to identify organisms from a positive blood culture bottle after only 4 h of growth on solid media compared to identification from overnight growth using the VITEK MS...
June 28, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28655999/genome-wide-identification-and-expression-analysis-of-amino-acid-transporters-in-the-whitefly-bemisia-tabaci-gennadius
#20
Jixing Xia, Zezhong Yang, Cheng Gong, Wen Xie, Huipeng Pan, Zhaojiang Guo, Huixin Zheng, Xin Yang, Xiaodong Sun, Shi Kang, Fengshan Yang, Qingjun Wu, Shaoli Wang, Bin Cong, Xianfeng Teng, Youjun Zhang
The whitefly (Bemisia tabaci) is a cosmopolitan and devastating pest of agricultural crops and ornamentals. B. tabaci causes extensive damage by feeding on phloem and by transmitting plant viruses. Like many other organisms, insects depend on amino acid transporters (AATs) to transport amino acids into and out of its cells. We present a genome-wide and transcriptome-wide investigation of the following two families of AATs in B. tabaci biotype B: amino acid/auxin permease (AAAP) and amino acid/polyamine/organocation (APC)...
2017: International Journal of Biological Sciences
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