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https://www.readbyqxmd.com/read/28336134/evaluation-of-matrix-assisted-laser-desorption-ionization-time-of-fight-mass-spectrometry-for-identification-of-345-clinical-isolates-of-aspergillus-species-from-11-korean-hospitals-comparison-with-molecular-identification
#1
Ju Heon Park, Jong Hee Shin, Min Ji Choi, Jin Un Choi, Yeon-Joon Park, Sook Jin Jang, Eun Jeong Won, Soo Hyun Kim, Seung Jung Kee, Myung Geun Shin, Soon Pal Suh
We evaluated the ability of the Filamentous Fungi Library 1.0 of the MALDI-TOF MS Biotyper system to identify 345 clinical Aspergillus isolates from 11 Korean hospitals. Compared with results of the internal transcribed spacer region sequencing, the frequencies of correct identification at the species-complex level were 94.5% and 98.8% with cutoff values of 2.0 and 1.7, respectively. Compared with results of β-tubulin gene sequencing, the frequencies of correct identification at the species level were 96.0% (cutoff 2...
January 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28334389/profiling-diuraphis-noxia-hemiptera-aphididae-transcript-expression-of-the-biotypes-sa1-and-sam-feeding-on-various-triticum-aestivum-varieties
#2
N F V Burger, E Venter, A-M Botha
The intimate relationship between an aphid and its host is mediated by the composition of the secreted saliva. In the present study, aphid heads were sampled and transcript profiling conducted after aphids were fed on their preference host and transferred to a variety of preference and nonpreference hosts. It was found that the virulent Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) biotype SAM was able to selectively up-regulate more transcripts when confronted with feeding on a variety of hosts, than was the case with the less virulent D...
March 3, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28334168/resistance-monitoring-for-eight-insecticides-on-the-sweetpotato-whitefly-hemiptera-aleyrodidae-in-china
#3
Shaoli Wang, Youjun Zhang, Xin Yang, Wen Xie, Qingjun Wu
The sweetpotato whitefly, Bemisia tabaci (Gennadius), is an important pest of many crops worldwide. Because control of B. tabaci still depends on the application of insecticides in China, monitoring the insecticide resistance of B. tabaci populations is essential for achieving control and for managing resistance. In this study, field populations of B. tabaci on vegetables were collected in three regions of China in 2011, 2012, and 2013. The resistance of these populations (all of which were determined to belong to biotype Q) to eight insecticides (abamectin, spinetoram, imidacloprid, thiamethoxam, acetamiprid, nitenpyram, chlorpyrifos, and bifenthrin) was assessed by the leaf-dip method...
March 1, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28332937/yersinia-spp-in-wild-rodents-and-shrews-in-finland
#4
Suvi Joutsen, Riikka Laukkanen-Ninios, Heikki Henttonen, Jukka Niemimaa, Liina Voutilainen, Eva R Kallio, Heikki Helle, Hannu Korkeala, Maria Fredriksson-Ahomaa
Yersinia enterocolitica and Yersinia pseudotuberculosis are important zoonotic bacteria causing human enteric yersiniosis commonly reported in Europe. All Y. pseudotuberculosis strains are considered pathogenic, while Y. enterocolitica include both pathogenic and nonpathogenic strains which can be divided into six biotypes (1A, 1B, and 2-5) and about 30 serotypes. The most common types causing yersiniosis in Europe are Y. enterocolitica bioserotypes 4/O:3 and 2/O:9. Strains belonging to biotype 1A are considered as nonpathogenic because they are missing important virulence genes like the attachment-invasion-locus (ail) gene in the chromosome and the virulence plasmid...
March 23, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28328023/identification-of-microorganisms-in-biofluids-of-individuals-with-periodontitis-and-chronic-kidney-disease-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#5
Levy Anderson César Alves, Rafael Celestino Souza, Taciana Mara Couto da Silva, Andreia Watanabe, Meriellen Dias, Maria Anita Mendes, Ana Lídia Ciamponi
RATIONALE: Chronic kidney disease (CKD) and periodontitis (PD) are important health issues. There is a large variety of microorganisms related to the pathogenesis of periodontitis, and optimising the time and the cost of laboratory assays to detect these organisms is highly valuable in the medical field. METHODS: Bacteria were isolated from saliva and oral biofilm of 30 adolescents and young adults with definite medical and dental diagnosis of CKD and PD, respectively, and proteins were extracted for microorganism identification by means of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) technique...
May 30, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28327669/pollen-mediated-gene-flow-from-glyphosate-resistant-common-waterhemp-amaranthus-rudis-sauer-consequences-for-the-dispersal-of-resistance-genes
#6
Debalin Sarangi, Andrew J Tyre, Eric L Patterson, Todd A Gaines, Suat Irmak, Stevan Z Knezevic, John L Lindquist, Amit J Jhala
Gene flow is an important component in evolutionary biology; however, the role of gene flow in dispersal of herbicide-resistant alleles among weed populations is poorly understood. Field experiments were conducted at the University of Nebraska-Lincoln to quantify pollen-mediated gene flow (PMGF) from glyphosate-resistant (GR) to -susceptible (GS) common waterhemp using a concentric donor-receptor design. More than 130,000 common waterhemp plants were screened and 26,199 plants were confirmed resistant to glyphosate...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28318563/characterization-of-enterococcus-species-isolated-from-marine-recreational-waters-by-maldi-tof-ms-and-rapid-id-api%C3%A2-20-strep-system
#7
Ana Paula Guarnieri Christ, Solange Rodrigues Ramos, Rodrigo Cayô, Ana Cristina Gales, Elayse Maria Hachich, Maria Inês Zanoli Sato
MALDI-TOF Mass Spectrometry Biotyping has proven to be a reliable method for identifying bacteria at the species level based on the analysis of the ribosomal proteins mass fingerprint. We evaluate the usefulness of this method to identify Enterococcus species isolated from marine recreational water at Brazilian beaches. A total of 127 Enterococcus spp. isolates were identified to species level by bioMérieux's API® 20 Strep and MALDI-TOF systems. The biochemical test identified 117/127 isolates (92%), whereas MALDI identified 100% of the isolates, with an agreement of 63% between the methods...
March 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28317294/comparative-genome-analysis-reveals-important-genetic-differences-among-serotype-o1-and-serotype-o2-strains-of-y-%C3%A2-ruckeri-and-provides-insights-into-host-adaptation-and-virulence
#8
Desirée Cascales, José A Guijarro, Ana I García-Torrico, Jessica Méndez
Despite the existence of a commercial vaccine routinely used to protect salmonids against Yersinia ruckeri, outbreaks still occur, mainly caused by nonmotile and lipase-negative strains (serotype O1 biotype 2). Moreover, epizootics caused by other uncommon serotypes have also been reported. At the moment, one of the main concerns for the aquaculture industry is the expanding range of hosts of this pathogen and the emergence of new biotypes and serotypes causing mortality in fish farms and against which the vaccine cannot protect...
March 20, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28298832/reconstruction-of-interdental-papilla-using-autogenous-bone-and-connective-tissue-grafts
#9
Santhanakrishnan Muthukumar, Pooja Ajit, Shiyamali Sundararajan, Suresh Ranga Rao
Previous studies have reported the management of Class I and II papillary defects, but knowledge on Class III defects, estimated to have a poor periodontal prognosis, remains minimal. In this case report, a Class III papillary defect reconstruction was attempted mainly since the patient reported with difficulty in phonetics. In Stage I, autogenous bone graft from the maxillary tuberosity and subepithelial connective tissue graft was augmented to decrease the distance between the interdental bone crest and contact point, simultaneously achieving a switch in the periodontal biotype...
July 2016: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/28298693/occurrence-of-clinical-genotype-vibrio-vulnificus-in-clam-samples-in-mangalore-southwest-coast-of-india
#10
M S Sangeetha, Malathi Shekar, M N Venugopal
Vibrio vulnificus is an opportunistic human pathogen causing gastroenteritis, wound infection and primary septicemia. V. vulnificus population has been divided into subpopulations based on their phenotype and genotype characteristics. In this study, 38.5% (10/26) of clam (Meretrix meretrix) samples obtained from Mangalore markets were seen to harbor V. vulnificus. Biochemical characterization of V. vulnificus isolates showed the strains to belong to Biotype 1 phenotype. Genotyping of strains using the 16S rRNA and virulence correlated gene (vcg) typing methods identified the isolates to be of 16S rRNA typeB and vcgC type respectively...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28295891/molecular-serotyping-virulence-gene-profiling-and-pathogenicity-of-streptococcus-agalactiae-isolated-from-tilapia-farms-in-thailand-by-multiplex-pcr
#11
Korntip Kannika, Duangjai Pisuttharachai, Prapansak Srisapoome, Janenuj Wongtavatchai, Hidehiro Kondo, Ikuo Hirono, Sasimanas Unajak, Nontawith Areechon
AIMS: This study aimed to biotype Streptococcus agalactiae isolated from tilapia farms in Thailand based on molecular biotyping methods and to determine the correlation between the serotype and virulence of bacteria. In addition to a biotyping (serotyping) technique based on multiplex PCR of cps genes, in this study, we developed multiplex PCR typing of Group B streptococcus (GBS) virulence genes to examine 3 clusters of virulence genes and their correlation to the pathogenicity of S...
March 13, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28283983/parthenium-weed-parthenium-hysterophorus-l-and-climate-change-the-effect-of-co2-concentration-temperature-and-water-deficit-on-growth-and-reproduction-of-two-biotypes
#12
Thi Nguyen, Ali Ahsan Bajwa, Sheldon Navie, Chris O'Donnell, Steve Adkins
Climate change will have a considerable impact upon the processes that moderate weed invasion, in particular to that of parthenium weed (Parthenium hysterophorus L.). This study evaluated the performance of two Australian biotypes of parthenium weed under a range of environmental conditions including soil moisture (100 and 50% of field capacity), atmospheric carbon dioxide (CO2) concentration (390 and 550 ppm), and temperature (35/20 and 30/15 °C/day/night). Measurements were taken upon growth, reproductive output, seed biology (fill, viability and dormancy) and soil seed longevity...
March 11, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28283072/presence-of-ail-and-ystb-genes-in-yersinia-enterocolitica-biotype-1a-isolates-from-game-animals-in-poland
#13
A Platt-Samoraj, K Syczyło, A Szczerba-Turek, A Bancerz-Kisiel, A Jabłoński, S Łabuć, J Pajdak, N Oshakbaeva, W Szweda
The pathogenicity of Yersinia enterocolitica is associated with the presence of plasmid and chromosomal virulence genes. Strains belonging to biotype 1A do not possess pYV plasmids, often harbour the ystB gene and usually lack the ail gene, which is the main virulence marker for Y. enterocolitica. The simultaneous presence of ail and ystB is uncommon. In this study, 21/218 (9.6%) biotype 1A Y. enterocolitica isolates from rectal swabs of wild boar (Sus scrofa; n = 18), red deer (Cervus elaphus; n = 2) and roe deer (Capreolus capreolus; n = 1) in Poland harboured both ail and ystB genes...
March 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28283005/-comparison-of-maldi-tof-and-16s-rrna-methods-in-identification-of-viridans-group-streptococci
#14
Serap Süzük Yıldız, Banu Kaşkatepe, Salih Altınok, Mustafa Çetin, Alper Karagöz, Sümeyra Savaş
Accurate identification of viridans group streptococci (VGS) frequently encountered as a causative agent of infective endocarditis is always a challenge for the clinical microbiology laboratory. Clinical microbiology laboratories generally use semi automatic/full automatic systems, molecular methods and also conventional methods for the identification of these bacteria. There are recent published studies that have used MALDI-TOF (Matrix Assisted Laser Ionization Mass Spectrometry-Time of Flight) systems in the identification of VGS...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28282915/implementing-the-bruker-maldi-biotyper-in-the-public-health-laboratory-for-c-botulinum-neurotoxin-detection
#15
Michael J Perry, Dominick A Centurioni, Stephen W Davis, George E Hannett, Kimberlee A Musser, Christina T Egan
Currently, the gold standard method for active botulinum neurotoxin (BoNT) detection is the mouse bioassay (MBA). A Centers for Disease Control and Prevention-developed mass spectrometry (MS)-based assay that detects active BoNT was successfully validated and implemented in a public health laboratory in clinical matrices using the Bruker MALDI-TOF MS (Matrix-assisted laser desorption ionization-time of flight mass spectrometry) Biotyper. For the first time, a direct comparison with the MBA was performed to determine MS-based assay sensitivity using the Bruker MALDI Biotyper...
March 9, 2017: Toxins
https://www.readbyqxmd.com/read/28279824/assessment-of-gingival-biotype-and-facial-hard-soft-tissue-dimensions-in-the-maxillary-anterior-teeth-region-using-cone-beam-computed-tomography
#16
Reza Amid, Mahdieh Mirakhori, Yaser Safi, Mahdi Kadkhodazadeh, Mahshid Namdari
OBJECTIVE: This study sought to assess the relationship between facial gingival and bone dimensions in maxillary anterior teeth region using cone beam computed tomography (CBCT). DESIGN: This study assessed 621 maxillary anterior teeth in 144 patients. In the sagittal plane, facial bone thickness (BT) and gingival thickness (GT) were measured at the crestal level and at 2, 4 and 6mm apical to the cementoenamel junction (CEJ). The dentogingival complex (DGC) dimensions and the distance from the CEJ to bone crest were also measured on CBCT scans...
March 1, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28262370/species-identification-of-streptococcus-bovis-group-isolates-causing-bacteremia-a-comparison-of-two-maldi-tof-ms-systems
#17
Charlotte N Agergaard, Elisa Knudsen, Rimtas Dargis, Xiaohui C Nielsen, Jens J Christensen, Ulrik S Justesen
This study compared two MALDI-TOF MS systems (Biotyper and VITEK MS) on clinical Streptococcus bovis group isolates (n=66). The VITEK MS gave fewer misidentifications and a higher rate of correct identifications than the Biotyper. Only the identification of S. lutetiensis by the VITEK MS was reliable. Additional optimization of the available system databases is needed.
February 20, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28254687/clostridium-innocuum-is-a-significant-vancomycin-resistant-pathogen-for-extra-intestinal-clostridial-infection
#18
Ju-Hsin Chia, Ye Feng, Lin-Hui Su, Tsu-Lan Wu, Chyi-Liang Chen, Yi-Hua Liang, Cheng-Hsun Chiu
OBJECTIVES: Extra-intestinal clostridial infection (EICI) is rare but can be fatal. Traditional phenotypic methods can only assign many of the Clostridium species to the genus level. METHODS: A total of 376 non-repetitive Clostridium isolates from sterile sites were collected and subjected to MALDI-TOF Biotyper analysis and 16S rRNA sequencing. Antimicrobial susceptibility was determined, and clinical characteristics of the patients were assessed. C. innocuum isolates were characterized by genome sequencing and genotyping...
February 27, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28247071/host-plant-variety-and-not-climate-determines-the-establishment-and-performance-of-aceria-lantanae-eriophyidae-a-biological-control-agent-of-lantana-camara-in-south-africa
#19
Ludzula Mukwevho, David Simelane, Terence Olckers
The flower-galling mite Aceria lantanae (Cook) (Trombidiformes: Eriophyidae) was released for the biological control of Lantana camara L. (Verbenaceae) in South Africa in 2007, but has displayed variable and patchy establishment throughout the weed's range. Surveys were undertaken in 2013-2014, both seasonally and during the mite's peak infestation periods, to determine the influence of climatic factors on its performance. Although there were seasonal differences in the percentages of mite-infested inflorescences, these did not differ significantly between altitudinal zones...
February 28, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28245796/gene-expression-and-plant-hormone-levels-in-two-contrasting-rice-genotypes-responding-to-brown-planthopper-infestation
#20
Changyan Li, Chao Luo, Zaihui Zhou, Rui Wang, Fei Ling, Langtao Xiao, Yongjun Lin, Hao Chen
BACKGROUND: The brown planthopper (BPH; Nilaparvata lugens Stål) is a destructive piercing-sucking insect pest of rice. The plant hormones salicylic acid (SA) and jasmonic acid (JA) play important roles in plant-pest interactions. Many isolated rice genes that modulate BPH resistance are involved in the metabolism or signaling pathways of SA, JA and ethylene. 'Rathu Heenati' (RH) is a rice cultivar with a high-level, broad-spectrum resistance to all BPH biotypes. Here, RH was used as the research material, while a BPH-susceptible rice cultivar 'Taichung Native 1' (TN1) was the control...
February 28, 2017: BMC Plant Biology
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