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https://www.readbyqxmd.com/read/28198407/comparative-proteomic-analysis-of-horseweed-conyza-canadensis-biotypes-identifies-candidate-proteins-for-glyphosate-resistance
#1
Fidel González-Torralva, Adrian P Brown, Stephen Chivasa
Emergence of glyphosate-resistant horseweed (Conyza canadensis) biotypes is an example of how unrelenting use of a single mode of action herbicide in agricultural weed control drives genetic adaptation in targeted species. While in other weeds glyphosate resistance arose from target site mutation or target gene amplification, the resistance mechanism in horseweed uses neither of these, being instead linked to reduced herbicide uptake and/or translocation. The molecular components underpinning horseweed glyphosate-resistance remain unknown...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28196886/replication-of-vibrio-cholerae-classical-ctx-phage
#2
Eun Jin Kim, Hyun Jin Yu, Je Hee Lee, Jae-Ouk Kim, Seung Hyun Han, Cheol-Heui Yun, Jongsik Chun, G Balakrish Nair, Dong Wook Kim
The toxigenic classical and El Tor biotype Vibrio cholerae serogroup O1 strains are generated by lysogenization of host-type-specific cholera toxin phages (CTX phages). Experimental evidence of the replication and transmission of an El Tor biotype-specific CTX phage, CTX-1, has explained the evolution of V. cholerae El Tor biotype strains. The generation of classical biotype strains has not been demonstrated in the laboratory, and the classical biotype-specific CTX phage, CTX-cla, is considered to be defective with regard to replication...
February 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28191542/loose-ends-in-the-eradication-of-helicobacter-pylori-infection
#3
Armando Peixoto, Marco Silva, Rui Gaspar, Rui Morais, Rosa Ramalho, Guilherme Macedo, João Santos-Antunes
The eradication of Helicobacter pylori is essential for prevention and treatment of various conditions associated with this infection. However, its effectiveness is limited and influenced by factors linked to the bacteria and the host. In particular, influence of the biotype, smoking, diabetes mellitus, and previous treatment failure in eradication is understudied. Our center proposed to evaluate these aspects in a real life cohort by applying a questionnaire with demographic and lifestyle variables in patients who consecutively underwent urease breath test after the eradication therapy...
December 1, 2016: Journal of Translational Internal Medicine
https://www.readbyqxmd.com/read/28188092/-isolation-of-pseudomonas-stutzeri-from-an-odontogenic-inflammatory-cyst-diagnostic-relevance
#4
Susana Molgatini, Eduardo Rey, Jorge Basilaki, Christian Mosca, Rafael Galante, Laura Gliosca
Pseudomonas stutzeri is distributed widely in the environment, and occupies different ecological niches. However, it is found in clinically relevant infections as an opportunistic pathogen. Isolation of P. stutzeri from an odontogenic inflammatory cyst is an uncommon microbiological finding that has not been reported to date. In the case presented here, the bacterium was isolated from surgical material obtained from excision of an inflammatory odontogenic cyst located in the tooth 1.2, and presenting with concomitant pulp necrosis...
February 7, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28187830/lack-of-cross-resistance-between-neonicotinoids-and-sulfoxaflor-in-field-strains-of-q-biotype-of-whitefly-bemisia-tabaci-from-eastern-china
#5
Wenlong Wang, Shaoli Wang, Guangjie Han, Yuzhou Du, Jianjun Wang
Control of Bemisia tabaci has depended primarily and heavily on insecticides, especially neonicotinoids. The novel sulfoximine insecticide sulfoxaflor exhibits high potency against a broad range of sap-feeding insect species, including those resistant to neonicotinoids. The resistance levels of Q-biotype B. tabaci field strains collected from 8 locations in eastern China to neonicotinoids and sulfoxaflor were investigated, and single nucleotide polymorphisms (SNPs) of nicotinic acetylcholine receptor β1 subunit gene (Btβ1) were detected...
March 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28187829/cross-resistance-patterns-to-acetolactate-synthase-als-inhibiting-herbicides-of-flixweed-descurainia-sophia-l-conferred-by-different-combinations-of-als-isozymes-with-a-pro-197-thr-mutation-or-a-novel-trp-574-leu-mutation
#6
Wei Deng, Qian Yang, Yongzhi Zhang, Hongtao Jiao, Yu Mei, Xuefeng Li, Mingqi Zheng
Acetolactate synthase (ALS) is the common target of ALS-inhibiting herbicides, and target-site ALS mutations are the main mechanism of resistance to ALS-inhibiting herbicides. In this study, ALS1 and ALS2 genes with full lengths of 2004bp and 1998bp respectively were cloned in individual plants of susceptible (S) or resistant (R) flixweed (Descurainia sophia L.) populations. Two ALS mutations of Pro-197-Thr and/or Trp-574-Leu were identified in plants of three R biotypes (HB24, HB30 and HB42). In order to investigate the function of ALS isozymes in ALS-inhibiting herbicide resistance, pHB24 (a Pro-197-Thr mutation in ALS1 and a wild type ALS2), pHB42 (a Trp-574-Leu mutation in ALS1 and a wild type ALS2) and pHB30 (a Trp-574-Leu mutation in ALS1 and a Pro-197-Thr mutation in ALS2) subpopulations individually homozygous for different ALS mutations were generated...
March 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28185382/autogenous-bone-block-grafting-provides-facial-implant-tissue-stability-long-term
#7
Fernando Verdugo, Agurne Uribarri, Antonio D'Addona
BACKGROUND: Mucosal recession (MR) and bone loss can compromise anterior implant esthetics. PURPOSE: To evaluate tissue stability and clinical outcomes of anterior implants augmented with autogenous block transplants long-term. MATERIALS AND METHODS: This prospective cross-sectional clinical study analyzed facial tissue recession of anterior implants augmented with autogenous bone blocks and compared them to adjacent teeth in forty patients 52 months post-augmentation...
February 10, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/28170450/zirconia-implants-as-an-alternative-to-titanium-a-systematic-review-and-meta-analysis
#8
Basel Elnayef, Aida Lázaro, Fernando Suárez-López Del Amo, Pablo Galindo-Moreno, Hom-Lay Wang, Jordi Gargallo-Albiol, Federico Hernández-Alfaro
PURPOSE: The aim of the present study was to systematically evaluate the marginal bone loss (MBL), success, and survival of zirconia (Zi) implants and compare them with the widely studied titanium (Ti) implants. MATERIALS AND METHODS: An electronic and manual literature search of several databases was performed by two independent reviewers for articles up to July 2015 that reported the use of Zi implants and survival, success, and MBL with at least 12 months' follow-up...
February 7, 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/28168237/evaluation-of-the-width-of-the-alveolar-bone-in-subjects-with-different-gingival-biotypes-a-prospective-cohort-study-using-cone-beam-computed-tomography
#9
Nathalie Frumkin, Shlomo Via, Avigdor Klinger
OBJECTIVE: To examine the correlation between the gingival biotype and the morphology of the alveolar bone in human subjects, assessing the clinical and the practical values of these findings. METHOD AND MATERIALS: Thirty-five subjects were enrolled. The labial plate thickness was measured by cone beam computed tomography (CBCT). The correlation between CBCT and probe transparency measurements was analyzed with regard to tooth type (canine or central incisor). Additional data analysis included possible correlation to gender and smoking status...
February 6, 2017: Quintessence International
https://www.readbyqxmd.com/read/28165831/paxvax-cvd-103-hgr-single-dose-live-oral-cholera-vaccine
#10
Myron M Levine, Wilbur H Chen, James B Kaper, Michael Lock, Lisa Danzig, Marc Gurwith
Cholera remains a problem in developing countries and a risk for travelers. Hypochlorhydria, blood group O, cardiac and renal disease increase the risk of developing cholera gravis. Oral vaccines containing inactivated Vibrio cholerae and requiring two doses are available in some countries. No cholera vaccine had been available for U.S. travelers for decades until 2016 when CVD 103-HgR (Vaxchora™), an oral live attenuated vaccine, was licensed by the U.S. FDA. Areas covered: Enduring protection following wild-type cholera provided the rationale to develop a single-dose live oral vaccine...
February 6, 2017: Expert Review of Vaccines
https://www.readbyqxmd.com/read/28161160/non-culture-based-identification-of-mastitis-causing-bacteria-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#11
Juliana Regina Barreiro, Juliano Leonel Gonçalves, Patrícia Aparecida Campos Braga, Aline Gerato Dibbern, Marcos Nogueira Eberlin, Marcos Veiga Dos Santos
The purpose of this study was to evaluate the detection limit of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for direct identification, without previous microbiological culture, of bovine mastitis-causing bacteria from milk samples. Milk samples (n = 15) were experimentally contaminated with Staphylococcus aureus, Streptococcus uberis, Streptococcus agalactiae, Streptococcus dysgalactiae, and Escherichia coli to have bacterial counts ranging from 10(3) to 10(9) cfu/mL...
February 1, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28139072/host-differentiation-and-variability-of-orobanche-crenata-populations-from-legume-species-in-morocco-as-revealed-by-cross-infestation-and-molecular-analysis
#12
Mounia Ennami, Fatima Zahra Briache, Fatima Gaboun, Rabha Abdelwahd, Lamiae Ghaouti, Loubna Belqadi, James Westwood, Rachid Mentag
BACKGROUND: Orobanche crenata represents a major biotic constraint to production of faba bean and lentil in Morocco. While this parasitic plant attacks both of these crops, the extent to which Orobanche biotypes specialize in parasitizing specific crops is unknown. To address this question, we studied O. crenata that grew on different hosts and quantified their host specificity to faba bean and lentil. The virulence of O. crenata populations on each host was investigated through field trials, pot and Petri dishes assays...
January 31, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28131373/molecular-epidemiological-investigation-of-brucella-melitensis-circulating-in-mongolia-by-mlva16
#13
Sung-Il Kang, Moon Her, Janchivdorj Erdenebaataar, Batbaatar Vanaabaatar, Hyorim Cho, So-Ra Sung, Jin Ju Lee, Suk Chan Jung, Yong Ho Park, Ji-Yeon Kim
Mongolia has a high incidence of brucellosis in human and animals due to livestock husbandry. To investigate the genetic characteristics of Mongolian B. melitensis, an MLVA (multi-locus variable-number tandem-repeat analysis)-16 assay was performed with 94 B. melitensis isolates. They were identified as B. melitensis biovar (bv.) 1 (67), 3 (10) and Rev. 1 vaccine strains (17) using a classical biotyping and multiplex PCR. In genotyping, three human isolates were grouped at 2 genotypes with sheep isolates, and it implies that B...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28124778/rapid-maldi-biotyper-based-identification-and-cluster-analysis-of-streptococcus-iniae
#14
Si Won Kim, Seong Won Nho, Se Pyeong Im, Jung Seok Lee, Jae Wook Jung, Jassy Mary S Lazarte, Jaesung Kim, Woo-Jai Lee, Jeong-Ho Lee, Tae Sung Jung
Streptococcus iniae causes severe mortalities among cultured marine species, especially in the olive flounder (Paralichthys olivaceus), which is economically important in Korea and Japan. Recently, there has been growing concern regarding the emergence of S. iniae as a zoonotic pathogen. Here, 89 S. iniae isolates obtained from diseased olive flounders collected from 2003 to 2008 in Jeju Island, South Korea, were characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28120937/single-basal-application-of-thiacloprid-for-the-integrated-management-of-meloidogyne-incognita-and-bemisia-tabaci-in-tomato-crops
#15
Sa Dong, Xiaofen Ren, Dianli Zhang, Xiaoxue Ji, Kaiyun Wang, Kang Qiao
Tomato growers commonly face heavy nematode (Meloidogyne incognita) and whitefly (B-biotype Bemisia tabaci) infestations, and previous studies demonstrated that thiacloprid could be used to control M. incognita and B. tabaci in cucumber. However, the efficacy of a single basal application of thiacloprid to control both pests and its effect on yield in tomato remains unknown. In this study, the potential of thiacloprid application to the soil for the integrated control of M. incognita and B. tabaci in tomato was evaluated in the laboratory and the field...
January 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28119478/rapid-direct-detection-of-carbapenemase-producing-enterobacteriaceae-in-clinical-urine-samples-by-maldi-tof-ms-analysis
#16
Marina Oviaño, Cecilia de la Luna Ramírez, Luis Pedro Barbeyto, Germán Bou
OBJECTIVES: Development of an automated MALDI-TOF MS-based method for the rapid, direct detection of carbapenemase-producing Enterobacteriaceae in clinical urine samples within 90 min of sample reception. METHODS: A total of 3041 urine samples were processed by flow cytometry, and a cut-off value of ≥1.5 × 10(5) bacteria/mL was used to select samples for the study. Following these criteria, 608 samples were selected for direct bacterial identification...
January 23, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28119370/epidemiology-and-characterization-of-escherichia-coli-outbreak-on-a-pig-farm-in-south-africa
#17
Arnold T Kanengoni, Ronald Thomas, Awoke K Gelaw, Evelyn Madoroba
An investigation of mortality of piglets through clinical signs, post-mortem, histopathology and bacteriological analyses revealed the causal organism to be Escherichia coli, mainly O149: K91: K88 which belongs to the enterotoxigenic (ETEC) biotypes. Molecular characterization and epidemiologic analysis elucidated it as shiga-toxin (ST) Escherichia coli resistant to ampicillin, cefotaxime, tetracycline, trimethoprim-sulfamethoxazole, tylosin and neomycin. Conventional PCR results detected genes for ST-2, adhesin involved in diffuse adherence (AIDA-1) and F18 fimbriae virulence factors...
January 24, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28117853/soft-and-hard-tissue-management-%C3%A2-in-the-treatment-of-a-discolored-%C3%A2-single-tooth-in-the-esthetic-zone
#18
David Garcia-Baeza, Ramon Garcia-Adamez, Carlos Saavedra
When treating the color of a single tooth in the esthetic zone, it is a complex task to achieve the same visual effect as the rest of the teeth. In addition to the problem of the clinical crown color, if the patient has a thin periodontal biotype it is necessary to manipulate both the hard and soft tissue to achieve a good esthetic result. To change the biotype, a connective tissue graft needs to be performed. The graft must be stabilized exactly where it is needed, and the recipient area modified. The tooth needs to receive an adequate preparation so as to make the restoration thick enough to naturally mask the treated tooth, while also ensuring long-term stability...
2017: International Journal of Esthetic Dentistry
https://www.readbyqxmd.com/read/28116553/performance-of-the-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-system-for-rapid-identification-of-streptococci-a-review
#19
REVIEW
W-T Fan, T-T Qin, R-R Bi, H-Q Kang, P Ma, B Gu
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been developed as a new type of soft ionization mass spectrometry in recent years. An increasing number of clinical microbiological laboratories consider it as an innovative approach for bacterial identification. This study was undertaken in order to evaluate the use of MALDI-TOF MS for rapid identification of the clinical streptococci. A systematic review was conducted based on a literature search of the Medline and Embase databases...
January 23, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28115548/the-fatty-acid-regulator-fadr-influences-the-expression-of-the-virulence-cascade-in-the-el-tor-biotype-of-vibrio-cholerae-by-modulating-the-levels-of-toxt-via-two-different-mechanisms
#20
Gabriela Kovacikova, Wei Lin, Ronald K Taylor, Karen Skorupski
: FadR is a master regulator of fatty acid (FA) metabolism that coordinates the pathways of FA degradation and biosynthesis in enteric bacteria. We show here that a ΔfadR mutation in the El Tor biotype of Vibrio cholerae prevents the expression of the virulence cascade by influencing both the transcription and post-translational regulation of the master virulence regulator ToxT. FadR is a transcriptional regulator that represses the expression of genes involved in FA degradation, activates the expression of genes involved in unsaturated FA (UFA) biosynthesis and also activates the expression of two operons involved in saturated FA (SFA) biosynthesis...
January 23, 2017: Journal of Bacteriology
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