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https://www.readbyqxmd.com/read/28632140/thermal-stability-and-rheological-properties-of-the-non-stick-caf1-biomaterial
#1
Yakup Ulusu, Gema Dura, Helen Waller, Matthew Benning, David Fulton, Jeremy Lakey, Daniel Thomas Peters
The ability to culture cells in three dimensions has many applications, from drug discovery to wound healing. 3D cell culture methods often require appropriate scaffolds that mimic the cellular environments of different tissue types. The choice of material from which these scaffolds are made is of paramount importance, as its properties will define the manner in which cells interact with the scaffold. Caf1 is a protein polymer that is secreted from its host organism, Yersinia pestis, to enable escape from phagocytosis...
June 20, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28630070/regulation-of-type-iii-secretion-of-translocon-and-effector-proteins-by-the-esab-esal-esam-complex-in-edwardsiella-tarda
#2
Lu Yi Liu, Pin Nie, Hong Bing Yu, Hai Xia Xie
The type III secretion system (T3SS) plays a crucial role in the pathogenesis of many Gram-negative bacteria, including Edwardsiella tarda, an important fish pathogen. Within the E. tarda T3SS, there are three proteins (EsaB/EsaL/EsaM) that are homologous to proteins present in many other bacteria, including SpiC/SsaL/SsaM in Salmonella, SepD/SepL/CesL in EPEC and EHEC, and YscB/YopN/SycN in Yersinia EsaL was found to interact with both EsaB and EsaM within the bacterial cell, as revealed by a co-immunoprecipitation assay...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28627490/genotyping-and-antimicrobial-resistance-genes-of-yersinia-ruckeri-isolates-from-rainbow-trout-farms
#3
Muhammed Duman, Soner Altun, Murat Cengiz, Izzet Burcin Saticioglu, Ayse Gul Buyukekiz, Pinar Sahinturk
In this study, we compared 142 Yersinia ruckeri isolates collected between 2013 and 2016 from 6 different regions in Turkey. A total of 18 different genogroups were found, though most of the isolates clustered into the same genogroup as serotype O1. As immunization of fish with inactivated Y. ruckeri by injection, immersion, or feeding provide minimal protection against Y. ruckeri infection in Turkey, many fish producers use antimicrobials unrestrictedly, resulting in antimicrobial resistance in aquatic pathogens...
June 19, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28617525/characterization-of-high-exopolysaccharide-producing-lactobacillus-strains-isolated-from-mustard-pickles-for-potential-probiotic-applications
#4
Jing-Yao Huang, Cheng-Yen Kao, We-Sin Liu, Tony J Fang
The aim of this study was to characterize high exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) isolated from mustard pickles in Taiwan for potential probiotic applications. Among 39 collected LAB strains, four most productive EPS-producing strains were selected for further analysis. Comparative analyses of 16S rDNA genes rpoA and pheS sequences demonstrated that these strains were members of Lactobacillus plantarum-group (LPG). NCD 2, NLD 4, SLC 13, and NLD 16 showed survival rates of 95.83% ± 0...
June 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28614901/x-ray-crystal-structures-of-the-pheromone-binding-domains-of-two-quorum-hindered-transcription-factors-yenr-of-yersinia-enterocolitica-and-cepr2-of-burkholderia-cenocepacia
#5
Youngchang Kim, Gekleng Chhor, Ching-Sung Tsai, Gabriel Fox, Chia-Sui Chen, Nathan J Winans, Robert Jedrzejczak, Andrzej Joachimiak, Stephen C Winans
The ability of LuxR-type proteins to regulate transcription is controlled by bacterial pheromones, N-acylhomoserine lactones (AHLs). Most LuxR-family proteins require their cognate AHLs for activity, and some of them require AHLs for folding and stability, and for protease-resistance. However, a few members of this family are able to fold, dimerize, bind DNA, and regulate transcription in the absence of AHLs; moreover, these proteins are antagonized by their cognate AHLs. One such protein is YenR of Yersinia enterocolitica, which is antagonized by N-3-oxohexanoyl-l-homoserine lactone (OHHL)...
June 14, 2017: Proteins
https://www.readbyqxmd.com/read/28614589/lactoferrin-protects-against-intestinal-inflammation-and-bacteria-induced-barrier-dysfunction-in-vitro
#6
Nina A Hering, Julia Luettig, Susanne M Krug, Stephanie Wiegand, Gabriele Gross, Eric A van Tol, Jörg D Schulzke, Rita Rosenthal
The iron-binding glycoprotein lactoferrin (LF) is naturally present in human breast milk. Several studies suggest that LF contributes to infant health and development owing to a variety of protective effects, including antimicrobial and anti-inflammatory features. Therefore, we aimed to elucidate its protective properties on intestinal epithelial barrier dysfunction induced by infection or inflammation using the human epithelial cell culture models HT-29/B6 and T84. During barrier perturbation induced by the proinflammatory cytokine tumor necrosis factor α (TNF-α), bovine LF restored tight junction (TJ) morphometry and inhibited TNF-α-induced epithelial apoptosis...
June 14, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28606812/novel-ctl-epitopes-identified-through-a-y-pestis-proteome-wide-analysis-in-the-search-for-vaccine-candidates-against-plague
#7
Anat Zvi, Shahar Rotem, Ayelet Zauberman, Uri Elia, Moshe Aftalion, Erez Bar-Haim, Emanuelle Mamroud, Ofer Cohen
The causative agent of Plague, Yersinia pestis, is a highly virulent pathogen and a potential bioweapon. Depending on the route of infection, two prevalent occurrences of the disease are known, bubonic and pneumonic. The latter has a high fatality rate. In the absence of a licensed vaccine, intense efforts to develop a safe and efficacious vaccine have been conducted, and humoral-driven subunit vaccines containing the F1 and LcrV antigens are currently under clinical trials. It is well known that a cellular immune response might have an essential additive value to immunity and protection against Y...
June 9, 2017: Vaccine
https://www.readbyqxmd.com/read/28602163/investigation-of-a-food-borne-outbreak-of-gastroenteritis-in-a-school-canteen-revealed-a-variant-of-sapovirus-genogroup-v-not-detected-by-standard-pcr-sollentuna-sweden-2016
#8
Maria-Pia Hergens, Joanna Nederby Öhd, Erik Alm, Helena H Askling, Sofia Helgesson, Mona Insulander, Nina Lagerqvist, Bo Svenungsson, Malin Tihane, Thomas Tolfvenstam, Per Follin
A food-borne outbreak of gastroenteritis with more than 650 suspected cases occurred in April 2016 in Sollentuna, Sweden. It originated in a school kitchen serving a total of 2,700 meals daily. Initial microbiological testing (for Campylobacter, Salmonella, Shigella, Yersinia, Giardia, Cryptosporidium, Entamoeba histolytica, adeno-, astro-, noro-, rota- and sapovirus) of stool samples from 15 symptomatic cases was negative, despite a clinical presentation suggestive of calicivirus. Analyses of the findings from both the Sollentuna municipality environmental team and a web-based questionnaire suggested that the source of the outbreak was the salad buffet served on 20 April, although no specific food item could be identified...
June 1, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28598346/application-of-long-amplicon-propidium-monoazide-pcr-to-assess-the-effects-of-temperature-and-background-microbiota-on-pathogens-in-river-water
#9
Avid Banihashemi, Michele I Van Dyke, Peter M Huck
The decay rates of enteric waterborne pathogens were evaluated following the introduction of Yersinia enterocolitica, Salmonella enterica, Campylobacter jejuni and Arcobacter butzleri into river water at different temperatures (5, 15 and 25°C) for a period of 28 days. To improve the accuracy of the results a molecular viability assay, long amplicon propidium monoazide-polymerase chain reaction (PMA-PCR), was used to quantify the viable cell concentration and results from PCR with and without PMA were compared...
June 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/28591979/investigation-into-the-antimicrobial-action-and-mechanism-of-a-novel-endogenous-peptide-%C3%AE-casein-197-from-human-milk
#10
Yanrong Fu, Chenbo Ji, Xiaohui Chen, Xianwei Cui, Xing Wang, Jie Feng, Yun Li, Rui Qin, Xirong Guo
A novel endogenous peptide cleaved from 197-213 AA of β-casein, named β-casein 197, was identified by tandem mass spectrometry. β-casein 197 constituted a significant proportion of the peptide content in preterm milk. This study investigated the antibacterial effects and mechanisms against common pathogenic bacteria. Six bacterial strains were selected for this study: Escherichia coli, Staphylococcus aureus, Yersinia enterocolitica, Listeria monocytogenes, Klebsiella pneumonia and Bacillus subtilis. After synthesis, serial twofold dilutions of β-casein 197 were added to select for sensitive bacteria...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28578732/thirty-years-of-human-infections-caused-by-yersinia-enterocolitica-in-northern-spain-1985-2014
#11
J M Marimon, R Figueroa, P Idigoras, M Gomariz, M Alkorta, G Cilla, E Pérez-Trallero
Yersinia enterocolitica infection is a zoonosis with worldwide distribution, gastroenteritis being by far the most common clinical manifestation of human infection. In Gipuzkoa, northern Spain, human Y. enterocolitica infections increased from the mid-1980s to the beginning of the 21st century (from 7·9 to 23·2 annual episodes per 100 000 population) to decrease to 7·2 annual episodes per 100 000 population in the last years of the study. The hospital admission rate due to yersiniosis during the last 15 years of the study was 7·3%...
June 5, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28578266/next-generation-sequencing-based-multigene-panel-for-high-throughput-detection-of-food-borne-pathogens
#12
Chiara Ferrario, Gabriele Andrea Lugli, Maria Cristina Ossiprandi, Francesca Turroni, Christian Milani, Sabrina Duranti, Leonardo Mancabelli, Marta Mangifesta, Giulia Alessandri, Douwe van Sinderen, Marco Ventura
Contamination of food by chemicals or pathogenic bacteria may cause particular illnesses that are linked to food consumption, commonly referred to as foodborne diseases. Bacteria are present in/on various foods products, such as fruits, vegetables and ready-to-eat products. Bacteria that cause foodborne diseases are known as foodborne pathogens (FBPs). Accurate detection methods that are able to reveal the presence of FBPs in food matrices are in constant demand, in order to ensure safe foods with a minimal risk of causing foodborne diseases...
May 3, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28571909/the-presence-of-contaminations-in-sewage-sludge-the-current-situation
#13
Krzysztof Fijalkowski, Agnieszka Rorat, Anna Grobelak, Malgorzata J Kacprzak
Sewage sludge/biosolids are by-wastes of municipal and industrial wastewater treatment. As sources of nutrients (C, N, P) they are widely used in intensive farming where large supplementation of organic matter to maintain fertility and enhance crop yields is needed. However, according to the report of European Commission published in 2010, only 39% of produced sewage sludge is recycled into agriculture in the European Union. This situation occurs mainly due to the fact, that the sewage sludge may contain a dangerous volume of different contaminants...
May 29, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28571687/ngs-virulome-analysis-of-a-yersinia-enterocolitica-subsp-palearctica-bioserotype-4-o-3-st18-isolated-from-human-blood-in-brazil
#14
Leonardo Alves Rusak, Ricardo Magrani Junqueira, Ernesto Hofer, Deyse Christina Vallim, Marise Dutra Asensi
Yersinia enterocolitica is a widespread Gram-negative bacterium that causes gastrointestinal disease and other clinical manifestations in humans. Potentially pathogenic Y. enterocolitica has been isolated in Brazil, from human, environmental, food, and animal sources. Herein we report a genome sequence of Y. enterocolitica subsp. palearctica strain YE 19, serotype O:3, biotype 4, sequence type 18, with virulence determinants isolated from human blood in Rio de Janeiro in 2005. The results corroborate other findings that this strain harbors a set of virulence determinants that could play a role in host pathoadaptation and may also justify the successful dissemination of bioserotype 4/O:3 in Brazil...
May 29, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28567210/potential-disease-transmission-from-wild-geese-and-swans-to-livestock-poultry-and-humans-a-review-of-the-scientific-literature-from-a-one-health-perspective
#15
Johan Elmberg, Charlotte Berg, Henrik Lerner, Jonas Waldenström, Rebecca Hessel
There are more herbivorous waterfowl (swans and geese) close to humans, livestock and poultry than ever before. This creates widespread conflict with agriculture and other human interests, but also debate about the role of swans and geese as potential vectors of disease of relevance for human and animal health. Using a One Health perspective, we provide the first comprehensive review of the scientific literature about the most relevant viral, bacterial, and unicellular pathogens occurring in wild geese and swans...
2017: Infection Ecology & Epidemiology
https://www.readbyqxmd.com/read/28559882/bacterial-root-microbiome-of-plants-growing-in-oil-sands-reclamation-covers
#16
Eduardo K Mitter, J Renato de Freitas, James J Germida
Oil sands mining in northern Alberta impacts a large footprint, but the industry is committed to reclaim all disturbed land to an ecologically healthy state in response to environmental regulations. However, these newly reconstructed landscapes may be limited by several factors that include low soil nutrient levels and reduced microbial activity. Rhizosphere microorganisms colonize plant roots providing hosts with nutrients, stimulating growth, suppressing disease and increasing tolerance to abiotic stress...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28558260/antimicrobial-resistance-and-plasmid-replicons-in-yersinia-enterocolitica-strains-isolated-in-brazil-in-30-years
#17
Miliane R Frazão, Leonardo N Andrade, Ana L C Darini, Juliana P Falcão
Some studies evaluated the resistance profile of the Y. enterocolitica strains isolated in diverse countries. However, in Brazil the isolation and the study of Y. enterocolitica are not common and therefore information about the antimicrobial resistance profile of this species in this country is scarce. Therefore, the aim of this study was to evaluate the antimicrobial resistance of Y. enterocolitica of biotypes 1A, 2 and 4 isolated from clinical and non-clinical sources between 1979 and 2012, in Brazil. This study showed that some Yersinia enterocolitica of different biotypes remain susceptible to antimicrobials used for gastroenteritis treatment...
May 27, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28545413/development-of-an-lps-based-elisa-for-diagnosis-of-yersinia-enterocolitica-o-3-infections-in-danish-patients-a-follow-up-study
#18
Tine Dalby, Eva Rasmussen, Peter Schiellerup, Karen Angeliki Krogfelt
BACKGROUND: The bacterium Yersinia enterocolitica causes gastroenteritis in humans. The study aimed to develop a diagnostic enzyme-linked immunosorbent assay (ELISA) for detection of Yersinia enterocolitica O:3 LPS antibodies in sera from Danish patients with suspected Yersinia enterocolitica O:3 gastrointestinal infection. As a part of this, antibody decay profiles after culture confirmed Yersinia enteritis were studied. RESULTS: An ELISA using Yersinia enterocolitica O:3 LPS as the coating antigen was developed for measuring IgA, IgG and IgM specific antibodies...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28544266/pcr-incorporation-of-polyoxometalate-modified-deoxynucleotide-triphosphates-and-their-application-in-molecular-electrochemical-sensing-of-yersinia-pestis
#19
Ciara K O'Sullivan, Mayreli Ortiz, Ahmed Debela Mehdi, Marketa Svobodova, Serge Thorimbert, Denis Lasage, Richard Cole, Bernold Hasenknopf
Redox-labeled nucleotides are of increasing interest for the fabrication of next generation molecular tools and should meet requirements of being thermally stable, sensitive, and compatible with polymerase-mediated incorporation whilst also being electrochemically discriminable. The synthesis and characterization of Keggin and Dawson polyoxometalate-deoxynucleotide (POM-dNTP) bioconjugates linked through 7-deaza-modified purines is described. The modified POM-dNTPs were used for polymerase based amplification of a DNA sequence specific for Yersinia pestis and the amplified DNA detected via an electrochemical DNA sensor...
May 22, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28542807/genetic-diversity-virulotyping-and-antimicrobial-resistance-susceptibility-of-yersinia-enterocolitica-isolated-from-pigs-and-porcine-products-in-malaysia
#20
Kwai Lin Thong, Lai Kuan Tan, Peck Toung Ooi
BACKGROUND: The objectives of this study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia. RESULTS: Thirty-two non-repeat Y. enterocolitica strains of three bioserotypes (3 variant/O:3, n=27; 1B/O:8, n=3; 1A/O:5, n=2) were analysed. About 90% of strains were multidrug-resistant with MAR index <0.2 and majority of the strains were resistant to nalidixic acid, clindamycin, ampicillin, ticarcillin, tetracycline and amoxicillin...
May 24, 2017: Journal of the Science of Food and Agriculture
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