Fabian M P Kaiser, Iga Janowska, Roberta Menafra, Melanie de Gier, Jakov Korzhenevich, Ingrid Pico-Knijnenburg, Indu Khatri, Ansgar Schulz, Taco W Kuijpers, Arjan C Lankester, Lukas Konstantinidis, Miriam Erlacher, Susan Kloet, Pauline A van Schouwenburg, Marta Rizzi, Mirjam van der Burg
Although absence of interleukin-7 (IL-7) signaling completely abrogates T and B lymphopoiesis in mice, patients with severe combined immunodeficiency caused by mutations in the IL-7 receptor α chain (IL-7Rα) still generate peripheral blood B cells. Consequently, human B lymphopoiesis has been thought to be independent of IL-7 signaling. Using flow cytometric analysis and single-cell RNA sequencing of bone marrow samples from healthy controls and patients who are IL-7Rα deficient, in combination with in vitro modeling of human B-cell differentiation, we demonstrate that IL-7R signaling plays a crucial role in human B lymphopoiesis...
September 28, 2023: Blood