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https://www.readbyqxmd.com/read/28314075/expert-views-on-biological-threat-characterization-for-the-u-s-government-a-delphi-study
#1
Crystal R Watson, Matthew C Watson, Gary Ackerman, Gigi Kwik Gronvall
Biological threat characterization (BTC) involves laboratory research conducted for the purpose of biological defense. BTC research is important for improving biological risk assessment and informing resource prioritization. However, there are also risks involved in BTC work, including potential for escape from the laboratory or the misuse of research results. Using a modified Delphi study to gather opinions from U.S. experts in biosecurity and biodefense, this analysis explores what principles and safeguards can maximize the benefits of BTC research and ensure that it is conducted safely and securely...
March 17, 2017: Risk Analysis: An Official Publication of the Society for Risk Analysis
https://www.readbyqxmd.com/read/28299529/modeling-and-simulation-in-dose-determination-for-biodefense-products-approved-under-the-fda-animal-rule
#2
Kimberly L Bergman, K Krudys, S K Seo, J Florian
Development of effective medical countermeasures for biodefense is vital to United States biopreparedness and response in the age of terrorism, both foreign and domestic. A traditional drug development pathway toward approval is not possible for most biodefense-related indications, creating the need for alternative development pathways such as the FDA's Animal Rule. Under this unique regulatory mechanism, FDA-approval is based on adequate and well-controlled animal studies when it is neither ethical nor feasible to conduct human efficacy studies...
March 15, 2017: Journal of Pharmacokinetics and Pharmacodynamics
https://www.readbyqxmd.com/read/28258144/construction-of-bacillus-thuringiensis-simulant-strains-suitable-for-environmental-release
#3
Sangjin Park, Changhwan Kim, Daesang Lee, Dong Hyun Song, Ki Cheol Cheon, Hong Suk Lee, Seong Joo Kim, Jee Cheon Kim, Sang Yup Lee
For a surrogate bacterium to be used in outdoor studies, it is important to consider environmental and human safety, and ease of detection. Recently, Bacillus thuringiensis, a popular bioinsecticide bacterium, is gaining attention as a surrogate bacterium for its use in biodefense. In this study, we constructed simulant strains of B. thuringiensis with enhanced characteristics for environmental studies. Through transposon mutagenesis, pigment genes were inserted into the chromosome, producing yellow-colored colonies for easy detection...
March 3, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28235018/characterization-of-pathogenesis-of-and-immune-response-to-burkholderia-pseudomallei-k96243-using-both-inhalational-and-intraperitoneal-infection-models-in-balb-c-and-c57bl-6-mice
#4
Jeremy J Bearss, Melissa Hunter, Jennifer L Dankmeyer, Kristen A Fritts, Christopher P Klimko, Chris H Weaver, Jennifer L Shoe, Avery V Quirk, Ronald G Toothman, Wendy M Webster, David P Fetterer, Joel A Bozue, Patricia L Worsham, Susan L Welkos, Kei Amemiya, Christopher K Cote
Burkholderia pseudomallei, the etiologic agent of melioidosis, is a Gram negative bacterium designated as a Tier 1 threat. This bacterium is known to be endemic in Southeast Asia and Northern Australia and can infect humans and animals by several routes. Inhalational melioidosis has been associated with monsoonal rains in endemic areas and is also a significant concern in the biodefense community. There are currently no effective vaccines for B. pseudomallei and antibiotic treatment can be hampered by non-specific symptomology and also the high rate of naturally occurring antibiotic resistant strains...
2017: PloS One
https://www.readbyqxmd.com/read/28117174/stability-and-pre-formulation-development-of-a-plant-produced-anthrax-vaccine-candidate
#5
R Mark Jones, Michael Burke, Devon Dubose, Jessica A Chichester, Slobodanka Manceva, April Horsey, Stephen J Streatfield, Jeff Breit, Vidadi Yusibov
Second generation anthrax vaccines focus on the use of recombinant protective antigen (rPA) to elicit a strong, toxin neutralizing antibody responses in immunized subjects. The main difference between the rPA vaccines compared to the current licensed vaccine, anthrax vaccine absorbed (AVA), is the rPA vaccines are highly purified preparations of only rPA. These second generation rPA vaccines strive to elicit strong immune responses with substantially fewer doses than AVA while provoking less side effects. Many of the rPA candidates have shown to be effective in pre-clinical studies, but most of the second generation molecules have stability issues which reduce their efficacy over time...
January 20, 2017: Vaccine
https://www.readbyqxmd.com/read/28107743/high-sensitivity-four-layer-polymer-fiber-optic-evanescent-wave-sensor
#6
Xin Xin, Nianbing Zhong, Qiang Liao, Yanyan Cen, Ruohua Wu, Zhengkun Wang
We present a novel four-layer structure consisting of bottom, second, third, and surface layers in the sensing region, for a D-shaped step-index fiber-optic evanescent wave (FOEW) sensor. To reduce the background noise, the surface of the longitudinal section in the D-shaped region is coated with a light-absorbing film. We check the morphologies of the second and surface layers, examine the refractive indices (RIs) of the third and surface layers, and analyze the composition of the surface layer. We also investigate the effects of the thicknesses and RIs of the third and surface layers and the LA film on the light transmission and sensitivity of the FOEW sensors...
May 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28070933/secretome-analysis-of-diarrhea-inducing-strains-of-escherichia-coli
#7
Raja Sekhar Nirujogi, Babylakshmi Muthusamy, Min-Sik Kim, Gajanan J Sathe, P T V Lakshmi, Olga N Kovbasnjuk, T S Keshava Prasad, Mary Wade, Rabih E Jabbour
Secreted proteins constitute a major part of virulence factors that are responsible for pathogenesis caused by Gram-negative bacteria. Enterohemorrhagic Escherichia coli, O157:H7, is the major pathogen often causing outbreaks. However, studies have reported that the significant outbreaks caused by non-O157:H7 E. coli strains, also known as "Big-Six" serogroup strains, are increasing. There is no systematic study describing differential secreted proteins from these non-O157:H7 E. coli strains. In this study, we carried out MS-based differential secretome analysis using tandem mass tags labeling strategy of non-O157:H7 E...
January 9, 2017: Proteomics
https://www.readbyqxmd.com/read/27918487/integrating-deoxyribozymes-into-colorimetric-sensing-platforms
#8
REVIEW
Dingran Chang, Sandy Zakaria, Mimi Deng, Nicholas Allen, Kha Tram, Yingfu Li
Biosensors are analytical devices that have found a variety of applications in medical diagnostics, food quality control, environmental monitoring and biodefense. In recent years, functional nucleic acids, such as aptamers and nucleic acid enzymes, have shown great potential in biosensor development due to their excellent ability in target recognition and catalysis. Deoxyribozymes (or DNAzymes) are single-stranded DNA molecules with catalytic activity and can be isolated to recognize a wide range of analytes through the process of in vitro selection...
December 3, 2016: Sensors
https://www.readbyqxmd.com/read/27775159/a-dual-purpose-universal-influenza-vaccine-candidate-confers-protective-immunity-against-anthrax
#9
Maria T Arévalo, Junwei Li, Diana Diaz-Arévalo, Yanping Chen, Ashley Navarro, Lihong Wu, Yongyong Yan, Mingtao Zeng
Preventive influenza vaccines must be reformulated annually because of antigen shift and drift of circulating influenza viral strains. However, seasonal vaccines do not always match the circulating strains, and there is the ever-present threat that avian influenza viruses may adapt to humans. Hence, a universal influenza vaccine is needed to provide protective immunity against a broad range of influenza viruses. We designed an influenza antigen consisting of three tandem M2e repeats plus HA2, in combination with a detoxified anthrax oedema toxin delivery system (EFn plus PA) to enhance immune responses...
March 2017: Immunology
https://www.readbyqxmd.com/read/27710702/state-of-the-art-advances-in-radiation-biodosimetry-for-mass-casualty-events-involving-radiation-exposure
#10
Mary Sproull, Kevin Camphausen
With the possibility of large-scale terrorist attacks around the world, the need for modeling and development of new medical countermeasures for potential future chemical, biological, radiological and nuclear (CBRN) has been well established. Project Bioshield, initiated in 2004, provided a framework to develop and expedite research in the field of CBRN exposures. To respond to large-scale population exposures from a nuclear event or radiation dispersal device (RDD), new methods for determining received dose using biological modeling became necessary...
November 2016: Radiation Research
https://www.readbyqxmd.com/read/27578439/epidemiology-and-management-of-the-2013-16-west-african-ebola-outbreak
#11
M L Boisen, J N Hartnett, A Goba, M A Vandi, D S Grant, J S Schieffelin, R F Garry, L M Branco
The 2013-16 West African Ebola outbreak is the largest, most geographically dispersed, and deadliest on record, with 28,616 suspected cases and 11,310 deaths recorded to date in Guinea, Liberia, and Sierra Leone. We provide a review of the epidemiology and management of the 2013-16 Ebola outbreak in West Africa aimed at stimulating reflection on lessons learned that may improve the response to the next international health crisis caused by a pathogen that emerges in a region of the world with a severely limited health care infrastructure...
September 29, 2016: Annual Review of Virology
https://www.readbyqxmd.com/read/27482881/a-role-for-science-in-responding-to-health-crises
#12
Leremy A Colf, Reginald Brothers, Christina E Murata
The Department of Homeland Security's (DHS) Science and Technology (S&T) Directorate plays a role in public health that extends beyond biodefense. These responsibilities were exercised as part of the 2014-16 Ebola outbreak, leading to productive and beneficial contributions to the international public health response and improved operations in the United States. However, we and others have identified numerous areas for improvement. Based on our successes and lessons learned, we propose a number of ways that DHS, the interagency, and academia can act now to ensure improved responses to future public health crises...
July 2016: Health Security
https://www.readbyqxmd.com/read/27412594/myristoylation-of-the-arenavirus-envelope-glycoprotein-stable-signal-peptide-is-critical-for-membrane-fusion-but-dispensable-for-virion-morphogenesis
#13
Joanne York, Jack H Nunberg
UNLABELLED: Arenaviruses are responsible for severe and often fatal hemorrhagic disease. In the absence of effective antiviral therapies and vaccines, these viruses pose serious threats to public health and biodefense. Arenaviruses enter the host cell by fusion of the viral and endosomal membranes, a process mediated by the virus envelope glycoprotein GPC. Unlike other class I viral fusion proteins, GPC retains its stable signal peptide (SSP) as an essential third subunit in the mature complex...
September 15, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27389971/agility-in-adversity-vaccines-on-demand
#14
Anne S De Groot, Leonard Moise, David Olive, Leo Einck, William Martin
Is the US ready for a biological attack using Ebola virus or Anthrax? Will vaccine developers be able to produce a Zika virus vaccine, before the epidemic spreads around the world? A recent report by The Blue Ribbon Study Panel on Biodefense argues that the US is not ready for these challenges, however, technologies and capabilities that could address these deficiencies are within reach. Vaccine technologies have advanced and readiness has improved in recent years, due to advances in sequencing technology and computational power making the 'vaccines on demand' concept a reality...
September 2016: Expert Review of Vaccines
https://www.readbyqxmd.com/read/27311112/a-high-sensitivity-fiber-optic-evanescent-wave-sensor-with-a-three-layer-structure-composed-of-canada-balsam-doped-with-geo2
#15
Nianbing Zhong, Mingfu Zhao, Lianchao Zhong, Qiang Liao, Xun Zhu, Binbin Luo, Yishan Li
In this paper, we present a high-sensitivity polymer fiber-optic evanescent wave (FOEW) sensor with a three-layer structure that includes bottom, inter-, and surface layers in the sensing region. The bottom layer and inter-layer are POFs composed of standard cladding and the core of the plastic optical fiber, and the surface layer is made of dilute Canada balsam in xylene doped with GeO2. We examine the morphology of the doped GeO2, the refractive index and composition of the surface layer and the surface luminous properties of the sensing region...
November 15, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27280174/fast-sensitive-point-of-care-electrochemical-molecular-system-for-point-mutation-and-select-agent-detection
#16
J A MacLeod, A C Nemeth, W C Dicke, D Wang, S Manalili Wheeler, J C Hannis, G B Collier, J J Drader
Point of care molecular diagnostics benefits from a portable battery-operated device capable of performing a fast turnaround using reliable inexpensive cartridges. We describe a prototype device for performing a molecular diagnostics test for clinical and biodefense samples in 16 minutes using a prototype capable of an 8 minute PCR reaction, followed by hybridization and detection on an electrochemical microarray based on the i-STAT® system. We used human buccal swabs for hemochromatosis testing including in-device DNA extraction...
July 7, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27259672/the-biodefense-field
#17
EDITORIAL
Emilio Mordini
No abstract text is available yet for this article.
July 2016: Bioethics
https://www.readbyqxmd.com/read/27224530/discovery-of-high-affinity-anti-ricin-antibodies-by-b-cell-receptor-sequencing-and-by-yeast-display-of-combinatorial-vh-vl-libraries-from-immunized-animals
#18
Bo Wang, Chang-Han Lee, Erik L Johnson, Christien A Kluwe, Josephine C Cunningham, Hidetaka Tanno, Richard M Crooks, George Georgiou, Andrew D Ellington
Ricin is a toxin that could potentially be used as a bioweapon. We identified anti-ricin A chain antibodies by sequencing the antibody repertoire from immunized mice and by selecting high affinity antibodies using yeast surface display. These methods led to the isolation of multiple antibodies with high (sub-nanomolar) affinity. Interestingly, the antibodies identified by the 2 independent approaches are from the same clonal lineages, indicating for the first time that yeast surface display can identify native antibodies...
August 2016: MAbs
https://www.readbyqxmd.com/read/27194767/small-molecule-fusion-inhibitors-bind-the-ph-sensing-stable-signal-peptide-gp2-subunit-interface-of-the-lassa-virus-envelope-glycoprotein
#19
Sundaresh Shankar, Landon R Whitby, Hedi E Casquilho-Gray, Joanne York, Dale L Boger, Jack H Nunberg
UNLABELLED: Arenavirus species are responsible for severe life-threatening hemorrhagic fevers in western Africa and South America. Without effective antiviral therapies or vaccines, these viruses pose serious public health and biodefense concerns. Chemically distinct small-molecule inhibitors of arenavirus entry have recently been identified and shown to act on the arenavirus envelope glycoprotein (GPC) to prevent membrane fusion. In the tripartite GPC complex, pH-dependent membrane fusion is triggered through a poorly understood interaction between the stable signal peptide (SSP) and the transmembrane fusion subunit GP2, and our genetic studies have suggested that these small-molecule inhibitors act at this interface to antagonize fusion activation...
August 1, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27159829/structural-analysis-of-nested-neutralizing-and-non-neutralizing-b-cell-epitopes-on-ricin-toxin-s-enzymatic-subunit
#20
Michael J Rudolph, David J Vance, Michael S Cassidy, Yinghui Rong, Charles B Shoemaker, Nicholas J Mantis
In this report, we describe the X-ray crystal structures of two single domain camelid antibodies (VH H), F5 and F8, each in complex with ricin toxin's enzymatic subunit (RTA). F5 has potent toxin-neutralizing activity, while F8 has weak neutralizing activity. F5 buried a total of 1760 Å(2) in complex with RTA and made contact with three prominent secondary structural elements: α-helix B (Residues 98-106), β-strand h (Residues 113-117), and the C-terminus of α-helix D (Residues 154-156). F8 buried 1103 Å(2) in complex with RTA that was centered primarily on β-strand h...
August 2016: Proteins
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