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https://www.readbyqxmd.com/read/27930677/de-novo-sequencing-and-comparative-analysis-of-schima-superba-seedlings-to-explore-the-response-to-drought-stress
#1
Bao-Cai Han, Wei Wei, Xiang-Cheng Mi, Ke-Ping Ma
Schima superba is an important dominant species in subtropical evergreen broadleaved forests of China, and plays a vital role in community structure and dynamics. However, the survival rate of its seedlings in the field is low, and water shortage could be a factor that limits its regeneration. In order to better understand the response of its seedlings to drought stress on a functional genomics scale, RNA-seq technology was utilized in this study to perform a large-scale transcriptome sequencing of the S. superba seedlings under drought stress...
2016: PloS One
https://www.readbyqxmd.com/read/27930666/development-of-pet-imaging-to-visualize-activated-macrophages-accumulated-in-the-transplanted-ipsc-derived-cardiac-myocytes-of-allogeneic-origin-for-detecting-the-immune-rejection-of-allogeneic-cell-transplants-in-mice
#2
Noriyuki Kashiyama, Shigeru Miyagawa, Satsuki Fukushima, Takuji Kawamura, Ai Kawamura, Shohei Yoshida, Akima Harada, Tadashi Watabe, Yasukazu Kanai, Koichi Toda, Jun Hatazawa, Yoshiki Sawa
Allogeneic transplantation (Tx) of induced pluripotent stem cells (iPSCs) is a promising tissue regeneration therapy. However, this inevitably induces macrophage-mediated immune response against the graft, limiting its therapeutic efficacy. Monitoring the magnitude of the immune response using imaging tools would be useful for prolonging graft survival and increasing the therapy longevity. Minimally invasive quantitative detection of activated macrophages by medical imaging technologies such as positron emission tomography (PET) imaging targets translocator protein (TSPO), which is highly expressed on mitochondrial membrane, especially in activated macrophage...
2016: PloS One
https://www.readbyqxmd.com/read/27928823/identification-of-differentially-expressed-genes-in-longissimus-muscle-of-pigs-with-high-and-low-intramuscular-fat-content-using-rna-sequencing
#3
K S Lim, K T Lee, J E Park, W H Chung, G W Jang, B H Choi, K C Hong, T H Kim
Intramuscular fat (IMF) content in pork is an important element of consumer preference and is positively correlated with meat quality, including tenderness and juiciness. With advances in RNA sequencing technologies, transcriptome-related differences can be associated with specific traits in animals. The objective of this study was to investigate differentially expressed genes (DEGs) closely related to IMF content in porcine longissimus muscle using RNA sequencing. A total of 107 Berkshire pigs were used for IMF content measurements, and significant differences between extremely high (H, n = 3) and low (L, n = 3) IMF content groups were found (P < 0...
December 7, 2016: Animal Genetics
https://www.readbyqxmd.com/read/27922848/lung-regeneration-steps-toward-clinical-implementation-and-use
#4
Elizabeth A Calle, Katherine L Leiby, MichaSam B Raredon, Laura E Niklason
PURPOSE OF REVIEW: Whole lung tissue engineering is a relatively new area of investigation. In a short time, however, the field has advanced quickly beyond proof of concept studies in rodents and now stands on the cusp of wide-spread scale up to large animal studies. Therefore, this technology is ever closer to being directly clinically relevant. RECENT FINDINGS: The main themes in the literature include refinement of the fundamental components of whole lung engineering and increasing effort to direct induced pluripotent stem cells and lung progenitor cells toward use in lung regeneration...
December 5, 2016: Current Opinion in Anaesthesiology
https://www.readbyqxmd.com/read/27909210/a-full-scale-study-on-thermal-degradation-of-polychlorinated-dibenzo-p-dioxins-and-dibenzofurans-in-municipal-solid-waste-incinerator-fly-ash-and-its-secondary-air-pollution-control-in-china
#5
Xingbao Gao, Bingjing Ji, Dahai Yan, Qifei Huang, Xuemei Zhu
Degradation of polychlorinated dibenzo-p-dioxins and dibenzofurans in municipal solid waste incinerator fly ash is beneficial to its risk control. Fly ash was treated in a full-scale thermal degradation system (capacity 1 t d(-1)) to remove polychlorinated dibenzo-p-dioxins and dibenzofurans. Apart from the confirmation of the polychlorinated dibenzo-p-dioxin and dibenzofuran decomposition efficiency, we focused on two major issues that are the major obstacles for commercialising this decomposition technology in China, desorption and regeneration of dioxins and control of secondary air pollution...
November 30, 2016: Waste Management & Research
https://www.readbyqxmd.com/read/27907131/itraq-based-quantitative-proteomic-comparison-of-early-and-late-passage-human-dermal-papilla-cell-secretome-in-relation-to-inducing-hair-follicle-regeneration
#6
Huan Zhang, Ning-Xia Zhu, Keng Huang, Bo-Zhi Cai, Yang Zeng, Yan-Ming Xu, Yang Liu, Yan-Ping Yuan, Chang-Min Lin
Alopecia is an exceedingly prevalent problem that lacks effective therapy. Recently, research has focused on early-passage dermal papilla cells (DPCs), which have hair inducing activity both in vivo and in vitro. Our previous study indicated that factors secreted from early-passage DPCs contribute to hair follicle (HF) regeneration. To identify which factors are responsible for HF regeneration and why late-passage DPCs lose this potential, we collected 48-h-culture medium (CM) from both of passage 3 and 9 DPCs, and subcutaneously injected the DPC-CM into NU/NU mice...
2016: PloS One
https://www.readbyqxmd.com/read/27900623/efficient-removal-of-antibiotics-in-a-fluidized-bed-reactor-by-facile-fabricated-magnetic-powdered-activated-carbon
#7
Jianqing Ma, Qunfeng Yang, Dongmei Xu, Xiaomei Zeng, Yuezhong Wen, Weiping Liu
Powdered activated carbons (PACs) with micrometer size are showing great potential for enabling and improving technologies in water treatment. The critical problem in achieving practical application of PAC involves simple, effective fabrication of magnetic PAC and the design of a feasible reactor that can remove pollutants and recover the adsorbent efficiently. Herein, we show that such materials can be fabricated by the combination of PAC and magnetic Fe3O4 with chitosan-Fe hydrogel through a simple co-precipitation method...
November 29, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27890224/new-frontiers-in-biomaterials
#8
REVIEW
R Gilbert Triplett, Oksana Budinskaya
Scientific and technological advances have combined to lead the way into a new era of the ever-developing science of biomaterials and tissue regeneration. This field has rapidly grown and new frontiers have quickly been established. Despite obtaining satisfactory results with current methods, improved techniques that lead to diminished patient discomfort, more favorable long-term prognosis, and decreased health care costs continue to be the goals of researchers, patients, and surgeons. Biomaterials have undergone a rapid evolution from materials that simply replaced tissues to factors that stimulate a biological response in the body...
February 2017: Oral and Maxillofacial Surgery Clinics of North America
https://www.readbyqxmd.com/read/27889241/natural-and-synthetic-coral-biomineralization-for-human-bone-revitalization
#9
REVIEW
David W Green, Besim Ben-Nissan, Kyung Sik Yoon, Bruce Milthorpe, Han-Sung Jung
Coral skeletons can regenerate replacement human bone in nonload-bearing excavated skeletal locations. A combination of multiscale, interconnected pores and channels and highly bioactive surface chemistry has established corals as an important alternative to using healthy host bone replacements. Here, we highlight how coral skeletal systems are being remolded into new calcified structures or synthetic corals by biomimetic processes, as places for the organized permeation of bone tissue cells and blood vessels...
November 23, 2016: Trends in Biotechnology
https://www.readbyqxmd.com/read/27880892/electrospinning-versus-microfluidic-spinning-of-functional-fibers-for-biomedical-applications
#10
REVIEW
Jie Cheng, Yesl Jun, Jianhua Qin, Sang-Hoon Lee
Micro- or nanofiber-based materials have extensive applications in biomedical fields due to their capability to mimic many aspects of physiological microenvironment in vivo. Fabricating micro- or nanofibers using biocompatible and biodegradable materials is becoming of great interest in the area of biomaterials and tissue engineering. Among the various technologies, electrospinning and microfluidic spinning are the two promising approaches to produce fibers at micro- and nano-scale. Choosing an appropriate spinning method is critical important for a specific application...
January 2017: Biomaterials
https://www.readbyqxmd.com/read/27878977/silk-fibroin-scaffolds-with-muscle-like-elasticity-support-in-vitro-differentiation-of-human-skeletal-muscle-cells
#11
Vishal Chaturvedi, Deboki Naskar, Beverley F Kinnear, Elizabeth Grenik, Danielle E Dye, Miranda D Grounds, Subhas C Kundu, Deirdre R Coombe
Human adult skeletal muscle has a limited ability to regenerate after injury and therapeutic options for volumetric muscle loss are few. Technologies to enhance regeneration of tissues generally rely upon bioscaffolds to mimic aspects of the tissue extracellular matrix (ECM). In the present study, silk fibroins from four Lepidoptera (silkworm) species engineered into three-dimensional scaffolds were examined for their ability to support the differentiation of primary human skeletal muscle myoblasts. Human skeletal muscle myoblasts (HSMMs) adhered, spread and deposited extensive ECM on all the scaffolds, but immunofluorescence and quantitative polymerase chain reaction analysis of gene expression revealed that myotube formation occurred differently on the various scaffolds...
November 22, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27875945/3d-printing-articular-cartilage-recapitulating-the-complexity-of-native-tissue
#12
Ting Guo, Josephine Lembong, Lijie Grace Zhang, John P Fisher
In the past few decades, the field of tissue engineering combined with rapid prototyping (RP) techniques has been successful in creating biological substitutes that mimic tissues. Its applications in regenerative medicine have drawn efforts in research from various scientific fields, diagnostics, and clinical translation to therapies. While some areas of therapeutics are well developed, such as skin replacement, many others such as cartilage repair can still greatly benefit from tissue engineering and RP due to the low success and / or inefficiency of current existing, often surgical treatments...
November 22, 2016: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/27872100/regeneration-of-cd8%C3%AE-%C3%AE-t-cells-from-t-cell-derived-ipsc-imparts-potent-tumor-antigen-specific-cytotoxicity
#13
Takuya Maeda, Seiji Nagano, Hiroshi Ichise, Keisuke Kataoka, Daisuke Yamada, Seishi Ogawa, Haruhiko Koseki, Toshio Kitawaki, Norimitsu Kadowaki, Akifumi Takaori-Kondo, Kyoko Masuda, Hiroshi Kawamoto
Although adoptive transfer of cytotoxic T lymphocytes (CTL) offer a promising cancer therapeutic direction, the generation of antigen-specific CTL from patients has faced difficulty in efficient expansion in ex vivo culture. To resolve this issue, several groups have proposed that induced pluripotent stem cell technology be applied for the expansion of antigen-specific CTL, which retain expression of the same T-cell receptor as original CTL. However, in these previous studies, the regenerated CTL are mostly of the CD8αα(+) innate type and have less antigen-specific cytotoxic activity than primary CTL...
December 1, 2016: Cancer Research
https://www.readbyqxmd.com/read/27871414/orthobiologics-in-the-foot-and-ankle
#14
REVIEW
H Thomas Temple, Theodore I Malinin
Many allogeneic biologic materials, by themselves or in combination with cells or cell products, may be transformative in healing or regeneration of musculoskeletal bone and soft tissues. By reconfiguring the size, shape, and methods of tissue preparation to improve deliverability and storage, unique iterations of traditional tissue scaffolds have emerged. These new iterations, combined with new cell technologies, have shaped an exciting platform of regenerative products that are effective and provide a bridge to newer and better methods of providing care for orthopedic foot and ankle patients...
December 2016: Foot and Ankle Clinics
https://www.readbyqxmd.com/read/27866105/pilot-scale-removal-and-recovery-of-dissolved-phosphate-from-secondary-wastewater-effluents-with-reusable-znfezr-adsorbent-fe3o4-sio2-particles-with-magnetic-harvesting
#15
Asya Drenkova-Tuhtan, Michael Schneider, Matthias Franzreb, Carsten Meyer, Carsten Gellermann, Gerhard Sextl, Karl Mandel, Heidrun Steinmetz
Advanced nanocomposite magnetic particles functionalized with ZnFeZr-adsorbent are developed, characterized and tested for the removal and recovery of phosphate directly from spiked secondary wastewater effluent (∼10 mg/L PO4-P). The phosphate loaded particles can be extracted from the liquid phase via magnetic separation, regenerated in a NaOH solution where phosphate desorption takes place, and reused in numerous cycles. Laboratory experiments demonstrate their reusability and stability in 60 consecutive adsorption/desorption runs where under optimal conditions > 90% total P-recovery efficiency is reached...
November 14, 2016: Water Research
https://www.readbyqxmd.com/read/27864735/environmental-analysis-of-waste-foundry-sand-via-life-cycle-assessment
#16
Jozef Mitterpach, Emília Hroncová, Juraj Ladomerský, Karol Balco
The aim of this manuscript is to provide an environmental assessment of the creation and use of waste foundry sand (WFS) via an LCA in a foundry for grey cast iron. A life cycle impact assessment was carried out using SimaPro 8. This environmental analysis assessed the impact of creating waste foundry sand (WFS) in a foundry, Hronec (Slovakia, Central Europe). According to BREF, this foundry is classified as an iron foundry with a production capacity greater than 20 t/day with processes typical for grey cast iron foundries...
November 18, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27862790/cytocompatible-polymer-grafting-from-individual-living-cells-by-atom-transfer-radical-polymerization
#17
Ji Yup Kim, Bong Soo Lee, Jinsu Choi, Beom Jin Kim, Ji Yu Choi, Sung Min Kang, Sung Ho Yang, Insung S Choi
A cytocompatible method of surface-initiated, activator regenerated by electron transfer, atom transfer radical polymerization (SI-ARGET ATRP) is developed for engineering cell surfaces with synthetic polymers. Dopamine-based ATRP initiators are used for both introducing the ATRP initiator onto chemically complex cell surfaces uniformly (by the material-independent coating property of polydopamine) and protecting the cells from radical attack during polymerization (by the radical-scavenging property of polydopamine)...
November 10, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27860372/biomaterials-and-culture-technologies-for-regenerative-therapy-of-liver-tissue
#18
Roman A Perez, Cho-Rok Jung, Hae-Won Kim
Regenerative approach has emerged to substitute the current extracorporeal technologies for the treatment of diseased and damaged liver tissue. This is based on the use of biomaterials that modulate the responses of hepatic cells through the unique matrix properties tuned to recapitulate regenerative functions. Cells in liver preserve their phenotype or differentiate through the interactions with extracellular matrix molecules. Therefore, the intrinsic properties of the engineered biomaterials, such as stiffness and surface topography, need to be tailored to induce appropriate cellular functions...
November 18, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27860335/development-of-a-synthetic-tissue-engineered-3d-printed-bioceramic-based-bone-graft-with-homogenously-distributed-osteoblasts-and-mineralizing-bone-matrix-in-vitro
#19
Doaa Adel-Khattab, Francesca Giacomini, Renate Gildenhaar, Georg Berger, Cynthia Gomes, Ulf Linow, Martin Hardt, Barbara Peleska, Jens Günster, Michael Stiller, Alireza Houshmand, Khaled Abdel Ghaffar, Ahmed Gamal, Mohamed El-Mofty, Christine Knabe
Over the last decade there have been increasing efforts to develop 3D scaffolds for bone tissue engineering from bioactive ceramics with 3D printing emerging as a promising technology. The overall objective of the present study was to generate a tissue engineered synthetic bone graft with homogenously distributed osteoblasts and mineralizing bone matrix in vitro, thereby mimicking the advantageous properties of autogenous bone grafts and facilitating usage for reconstructing segmental discontinuity defects in vivo...
November 15, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27857193/a-self-regenerable-soot-sensor-with-a-proton-conductive-thin-electrolyte-and-a-nanostructured-platinum-sensing-electrode
#20
Peiling Lv, Takenori Ito, Akihide Oogushi, Kensaku Nakashima, Masahiro Nagao, Takashi Hibino
In recent years, exhaust sensors have become increasingly attractive for use in energy and environmental technologies. Important issues regarding practical applications of these sensors, especially for soot measurements, include the further development of ion-conductive electrolytes and active electrode catalysts for meeting performance and durability requirements. Herein, we design a proton conductor with a high breakdown voltage and a sensing electrode with high sensitivity to electrochemical carbon oxidation, enabling continuous soot monitoring with self-regeneration of the sensor...
November 18, 2016: Scientific Reports
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