keyword
MENU ▼
Read by QxMD icon Read
search

MPJ

keyword
https://www.readbyqxmd.com/read/27913980/complete-nucleotide-sequences-and-construction-of-full-length-infectious-cdna-clones-of-cucumber-green-mottle-mosaic-virus-cgmmv-in-a-versatile-newly-developed-binary-vector-including-both-35s-and-t7-promoters
#1
Chan-Hwan Park, Hye-Kyoung Ju, Jae-Yeong Han, Jong-Seo Park, Ik-Hyun Kim, Eun-Young Seo, Jung-Kyu Kim, John Hammond, Hyoun-Sub Lim
Seed-transmitted viruses have caused significant damage to watermelon crops in Korea in recent years, with cucumber green mottle mosaic virus (CGMMV) infection widespread as a result of infected seed lots. To determine the likely origin of CGMMV infection, we collected CGMMV isolates from watermelon and melon fields and generated full-length infectious cDNA clones. The full-length cDNAs were cloned into newly constructed binary vector pJY, which includes both the 35S and T7 promoters for versatile usage (agroinfiltration and in vitro RNA transcription) and a modified hepatitis delta virus ribozyme sequence to precisely cleave RNA transcripts at the 3' end of the tobamovirus genome...
December 2, 2016: Virus Genes
https://www.readbyqxmd.com/read/27728949/fenestrated-endografts-for-complex-abdominal-aortic-aneurysm-repair
#2
Arne de Niet, Michel Mpj Reijnen, Ignace Fj Tielliu, Jan Willem Hp Lardenoije, Clark J Zeebregts
Since the introduction of fenestrated endovascular aneurysm repair (FEVAR) in 1996, great advances have been made in endograft development. Custom-made and off-the-shelf fenestrated and branched endografts have been used to treat patients with complex abdominal aortic and thoraco-abdominal aneurysms. Most experience has been gained with the Cook Zenith® fenestrated endograft (Cook Medical Inc., Limerick, Ireland). The Cook Zenith® endograft is customized with fenestrations, (fixed) inner or outer branches, or a combination of them, to cover a wide range of complex aneurysms...
October 6, 2016: Surgical Technology International
https://www.readbyqxmd.com/read/27665270/contribution-of-secondary-igkappa-rearrangement-to-primary-immunoglobulin-repertoire-diversification
#3
Shufang Li, Wei Liu, Yinghui Li, Shaorong Zhao, Can Liu, Mengyun Hu, Wei Yue, Yanhua Liu, Yue Wang, Rongcun Yang, Rong Xiang, Feifei Liu
Abs reactive to DNA and DNA/histone complexes are a distinguished characteristic of primary immunoglobulin repertoires in autoimmune B6.MRL-Fas(lpr) and MRL/MpJ-Fas(lpr) mice. These mice are defective in Fas receptor, which is critical for the apoptosis of autoreactive B cells by an extrinsic pathway. In the present study, we explored the possibility that bone marrow small pre-B and immature B cells from adult B6.MRL-Fas(lpr) mice and MRL/MpJ-Fas(lpr) mice respectively, which contain autoreactive B-cell antigen receptors (BCR) and manifest autoimmune syndromes, exhibit enhanced receptor editing patterns...
October 2016: Molecular Immunology
https://www.readbyqxmd.com/read/27604104/strain-differences-in-the-proteome-of-dioxin-sensitive-and-dioxin-resistant-mice-treated-with-2-3-7-8-tetrabromodibenzo-p-dioxin
#4
Hoa Thanh Nguyen, Maria Claret Lauan Tsuchiya, Jean Yoo, Midori Iida, Tetsuro Agusa, Masashi Hirano, Eun-Young Kim, Tatsuhiko Miyazaki, Masato Nose, Hisato Iwata
Dioxins cause various toxic effects through the aryl hydrocarbon receptor (AHR) in vertebrates, with dramatic species and strain differences in susceptibility. Although inbred mouse strains C3H/HeJ-lpr/lpr (C3H/lpr) and MRL/MpJ-lpr/lpr (MRL/lpr) are known as dioxin-sensitive and dioxin-resistant mice, respectively, the molecular mechanism underlying this difference remains unclear. The difference in the hepatic proteome of the two mouse strains treated with vehicle or 2,3,7,8-tetrabromodibenzo-p-dioxin (TBDD) was investigated by a proteomic approach of two-dimensional electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry (MALDI-TOF/TOF)...
September 7, 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27586091/the-nellix%C3%A2-endovascular-sealing-system-in-patients-with-abdominal-aortic-aneurysms-in-conjunction-with-iliac-artery-occlusive-disease
#5
Steven Mm van Sterkenburg, Leo H van den Ham, Luuk Smeets, Jan-Willem Lardenoije, Michel Mpj Reijnen
INTRODUCTION: Concomitant abdominal aortic aneurysm formation and aortoiliac occlusive disease is a challenging combination, often requiring open reconstructive surgery. In this study, we have assessed a single center experience of the Nellix EndoVascular Aneurysm Sealing System in the treatment of an abdominal aortic aneurysm in conjunction with iliac artery occlusive disease. METHODS: Retrospectively case files of patients treated with Nellix EndoVascular Aneurysm Sealing System in a single center were reviewed...
August 31, 2016: Vascular
https://www.readbyqxmd.com/read/27552106/the-lg-j-murine-strain-exhibits-near-normal-tendon-biomechanical-properties-following-a-full-length-central-patellar-tendon-defect
#6
Jessica R Arble, Andrea L Lalley, Nathaniel A Dyment, Pujan Joshi, Dong-Guk Shin, Cynthia Gooch, Brian Grawe, David Rowe, Jason T Shearn
PURPOSE OF THE STUDY: Identifying biological success criteria is needed to improve therapies, and one strategy for identifying them is to analyze the RNA transcriptome for successful and unsuccessful models of tendon healing. We have characterized the MRL/MpJ murine strain and found improved mechanical outcomes following a central patellar tendon (PT) injury. In this study, we evaluate the healing of the LG/J murine strain, which comprises 75% of the MRL/MpJ background, to determine if the LG/J also exhibits improved biomechanical properties following injury and to determine differentially expressed transcription factors across the C57BL/6, MRL/MpJ and the LG/J strains during the early stages of healing...
August 23, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27475620/is-passive-metatarsophalangeal-joint-stiffness-related-to-leg-stiffness-vertical-stiffness-and-running-economy-during-sub-maximal-running
#7
Hok Sum Man, Wing Kai Lam, Justin Lee, Catherine M Capio, Aaron Kam Lun Leung
This study examined whether passive metatarsophalangeal joints (MPJ) stiffness was associated with leg stiffness (Kleg) vertical stiffness (Kvert) and running economy (RE) during sub-maximal running. Nine male experienced runners underwent passive MPJ stiffness measurements in standing and sitting positions followed by sub-maximal running on an instrumented treadmill. With the individual foot position properly aligned, the MPJ passive stiffness in both sitting (MPJsit) and standing positions (MPJstand) were measured with a computerized dynamometer...
September 2016: Gait & Posture
https://www.readbyqxmd.com/read/27356751/in-vitro-studies-implicate-an-imbalanced-activation-of-dendritic-cells-in-the-pathogenesis-of-murine-autoimmune-pancreatitis
#8
Luise Borufka, Erik Volmer, Sarah Müller, Robby Engelmann, Horst Nizze, Saleh Ibrahim, Robert Jaster
OBJECTIVES: MRL/MpJ mice spontaneously develop an autoimmune pancreatitis (AIP) and are widely used as a model to study the genetic, molecular and immunological basis of the disease. Here, we have addressed the question whether distinctive features of their dendritic cells (DCs) may predispose MRL/MpJ mice to the chronic inflammation. METHODS: Pancreatic lesions were analyzed employing histological methods. Cohorts of young (healthy) MRL/MpJ mice, adult (sick) individuals, and AIP-resistant CAST/EiJ mice were used to establish cultures of bone marrow (BM)-derived conventional DCs (cDCs)...
June 23, 2016: Oncotarget
https://www.readbyqxmd.com/read/27354685/estrogen-induced-expression-of-tumor-necrosis-factor-like-weak-inducer-of-apoptosis-through-er%C3%AE-accelerates-the-progression-of-lupus-nephritis
#9
Leixi Xue, Zhiqin Liu, Ji Hu, Jun Huang, Jian Wen, Zhichun Liu
OBJECTIVES: Oestrogens have been shown to play key roles in the pathogenesis of SLE. The aim of this study was to investigate the roles and mechanisms of 17β-estradiol (E2) in TNF-like weak inducer of apoptosis (TWEAK) expression in LN. METHODS: Peripheral blood mononuclear cells (PBMCs) obtained from LN patients were used for in vitro experiments, while female MRL/lpr and MRL/MpJ mice were used for in vivo studies. E2, ICI 182 780 [estrogen receptor (ER)-selective antagonist], methyl-piperidino-pyrazole (MPP, ERα-selective modulator), lentivirus (LV)-TWEAK-short hairpin RNA (shRNA) and LV-control-shRNA treatments were used in this study...
October 2016: Rheumatology
https://www.readbyqxmd.com/read/27334976/a-cross-sectional-observational-study-comparing-foot-and-ankle-characteristics-in-people-with-stroke-and-healthy-controls
#10
Dorit Kunkel, Julia Potter, Louis Mamode
PURPOSE: The purpose of this study was to explore and compare foot and ankle characteristics in people with stroke and healthy controls; and between stroke fallers and non-fallers. METHODS: Participants were recruited from community groups and completed standardized tests assessing sensation, foot posture, foot function, ankle dorsiflexion and first metatarsal phalangeal joint range of motion (1st MPJ ROM), hallux valgus presence and severity. RESULTS: Twenty-three stroke participants (mean age 75...
June 23, 2016: Disability and Rehabilitation
https://www.readbyqxmd.com/read/27311864/complex-dorsal-metacarpophalangeal-dislocation-long-term-follow-up
#11
Guy Rubin, Hagay Orbach, Micha Rinott, Nimrod Rozen
PURPOSE: To describe the long-term follow-up results of complex dorsal metacarpophalangeal joint dislocation (MPJD). We hypothesize that there would be no long-term functional deficit in most patients, even with the presence of one of the familiar complications. METHODS: We describe 5 patients with a median follow-up of 13 (range, 7-36) years and review the literature focusing on follow-up and complications. RESULTS: All patients reported full function of the hand...
August 2016: Journal of Hand Surgery
https://www.readbyqxmd.com/read/27278094/rel-b-modified-dendritic-cells-possess-tolerogenic-phenotype-and-functions-on-lupus-splenic-lymphocytes-in%C3%A2-vitro
#12
Haijing Wu, Yi Lo, Albert Chan, Ka Sin Law, Mo Yin Mok
Systemic lupus erythematosus (SLE) is an autoimmune disease that is characterized by high morbidity and mortality and its treatment remains challenging. Dendritic cells (DCs) have been shown to participate in the initiation and perpetuation of lupus pathogenesis and the DCs that can induce tolerogenicity appear as potential cell-based therapy in this condition. In this study, we examined the in vitro tolerogenic properties of bone-marrow derived DCs (BMDCs) in the murine lupus setting. We used lentiviral transduction of RelB-silencing short hairpin RNA to modify the expression of RelB, a key transcription factor regulating DC maturation, in BMDCs from MRL/MpJ mice...
September 2016: Immunology
https://www.readbyqxmd.com/read/27213693/c5a-induces-caspase-dependent-apoptosis-in-brain-vascular-endothelial-cells-in-experimental-lupus
#13
Supriya D Mahajan, Vincent M Tutino, Yonas Redae, Hui Meng, Adnan Siddiqui, Trent M Woodruff, James N Jarvis, Teresa Hennon, Stanley Schwartz, Richard J Quigg, Jessy J Alexander
Blood-brain barrier (BBB) dysfunction complicates central nervous system lupus, an important aspect of systemic lupus erythematosus. To gain insight into the underlying mechanism, vascular corrosion casts of brain were generated from the lupus mouse model, MRL/lpr mice and the MRL/MpJ congenic controls. Scanning electron microscopy of the casts showed loss of vascular endothelial cells in lupus mice compared with controls. Immunostaining revealed a significant increase in caspase 3 expression in the brain vascular endothelial cells, which suggests that apoptosis could be an important mechanism causing cell loss, and thereby loss of BBB integrity...
August 2016: Immunology
https://www.readbyqxmd.com/read/27109826/comparative-analysis-of-ear-hole-closure-identifies-epimorphic-regeneration-as-a-discrete-trait-in-mammals
#14
Thomas R Gawriluk, Jennifer Simkin, Katherine L Thompson, Shishir K Biswas, Zak Clare-Salzler, John M Kimani, Stephen G Kiama, Jeramiah J Smith, Vanessa O Ezenwa, Ashley W Seifert
Why mammals have poor regenerative ability has remained a long-standing question in biology. In regenerating vertebrates, injury can induce a process known as epimorphic regeneration to replace damaged structures. Using a 4-mm ear punch assay across multiple mammalian species, here we show that several Acomys spp. (spiny mice) and Oryctolagus cuniculus completely regenerate tissue, whereas other rodents including MRL/MpJ 'healer' mice heal similar injuries by scarring. We demonstrate ear-hole closure is independent of ear size, and closure rate can be modelled with a cubic function...
2016: Nature Communications
https://www.readbyqxmd.com/read/27054319/the-role-of-arch-compression-and-metatarsophalangeal-joint-dynamics-in-modulating-plantar-fascia-strain-in-running
#15
Kirsty A McDonald, Sarah M Stearne, Jacqueline A Alderson, Ian North, Neville J Pires, Jonas Rubenson
Elastic energy returned from passive-elastic structures of the lower limb is fundamental in lowering the mechanical demand on muscles during running. The purpose of this study was to investigate the two length-modulating mechanisms of the plantar fascia, namely medial longitudinal arch compression and metatarsophalangeal joint (MPJ) excursion, and to determine how these mechanisms modulate strain, and thus elastic energy storage/return of the plantar fascia during running. Eighteen runners (9 forefoot and 9 rearfoot strike) performed three treadmill running trials; unrestricted shod, shod with restricted arch compression (via an orthotic-style insert), and barefoot...
2016: PloS One
https://www.readbyqxmd.com/read/27040769/superior-angiogenesis-facilitates-digit-regrowth-in-mrl-mpj-mice-compared-to-c57bl-6-mice
#16
Alexander Kwiatkowski, Mark Piatkowski, Miao Chen, Lijuan Kan, Qingshu Meng, Huimin Fan, Abdel-Hamid K Osman, Zhongmin Liu, Benjamin Ledford, Jia-Qiang He
Previous studies indicated that the fast-healer strain of MRL/MpJ-Fas(lpr)/J (MRL) mice demonstrated superior regenerative capabilities for digit wound healing and/or regeneration compared with the non-healer strain of C57BL/6 (C57) mice. These reports, however, mainly focused on morphological observations and analysis of gene expression with little attention on the role of angiogenesis in the amputated digits. By taking advantage of Laser Doppler Imaging and histological analysis, we examined the potential role(s) of angiogenesis in facilitating tissue regrowth/regeneration by comparing two strains of mice (MRL versus C57)...
May 13, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26983696/intra-articular-injection-of-synovial-mesenchymal-stem-cells-improves-cartilage-repair-in-a-mouse-injury-model
#17
J Mak, C L Jablonski, C A Leonard, J F Dunn, E Raharjo, J R Matyas, J Biernaskie, R J Krawetz
Controversy remains whether articular cartilage has an endogenous stem/progenitor cell population, since its poor healing capacity after injury can lead to diseases such as osteoarthritis. In the joint environment there are mesenchymal stem/progenitor cells (MSCs) in the synovial membrane and synovial fluid that can differentiate into cartilage, but it is still under debate if these cells contribute to cartilage repair in vivo. In this study, we isolated a Sca-1 positive, chondrogenesis capable population of mouse synovial MSCs from C57BL6 and MRL/MpJ "super-healer" strains...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26837069/treatment-with-anti-hmgb1-monoclonal-antibody-does-not-affect-lupus-nephritis-in-mrl-lpr-mice
#18
Fleur Schaper, Mirjan M Van Timmeren, Arjen Petersen, Gerda Horst, Marc Bijl, Pieter C Limburg, Johanna Westra, Peter Heeringa
OBJECTIVE: High Mobility Group Box 1 (HMGB1) is a nuclear DNA binding protein which acts as an alarmin when secreted. HMGB1 is increased in SLE and might represent a potential therapeutic target. We investigated whether treatment with a anti-HMGB1 antibody affects the development of lupus nephritis in MRL/lpr mice. MATERIALS AND METHODS: Seven week old MRL/lpr mice were injected intraperitoneally twice weekly for 10 weeks with 50 μg monoclonal anti-HMGB1 (2G7, mouse IgG2b) (n=12) or control antibody (n=11)...
January 13, 2016: Molecular Medicine
https://www.readbyqxmd.com/read/26808773/heterotopic-vascularized-joint-transfer-in-mutilating-hand-injuries
#19
Nai-Jen Chang, Johnny T Chang, Chung-Chen Hsu, Cheng-Hung Lin, Chih-Hung Lin, Yu-Te Lin
BACKGROUND: In cases of mutilating hand injuries, the primary goal is recovery of prehensile function. This is particularly true in the case of joints, which are extremely difficult to replace or reconstruct adequately when damaged. Heterotopic vascularized joint transfer is indicated when salvageable joints are available for transfer to a more functionally optimal position on the hand. MATERIALS AND METHODS: Seven cases of mutilating hand injuries treated with heterotopic vascularized joint transfers from 2003 to 2012 were retrospectively identified...
March 2016: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/26646657/treatment-of-infantile-hemangiomas-therapeutic-options-in-regard-to-side-effects-and-adverse-events-a-review-of-the-literature
#20
Martine F Raphael, Johannes M P J Breur, Florine A E Vlasveld, Niels J Elbert, Yves T B Liem, Moshe Kon, Corstiaan C Breugem, Suzanne G M A Pasmans
INTRODUCTION: While options for treatment strategies for infantile hemangiomas (IH) are numerous, evidence-based information about agents, optimal dosage, adverse effects, treatment modality, pretreatment and treatment strategies remain limited. AREAS COVERED: To evaluate side effects and adverse events of medical treatment in children with infantile hemangioma, a comprehensive review of the literature was performed to provide information for daily practice. In total 254 studies were retrieved from medical databases and comprised 10,022 patients divided into 5 different treatment groups...
2016: Expert Opinion on Drug Safety
keyword
keyword
24844
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"