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https://www.readbyqxmd.com/read/29338557/biosynthesis-characterization-and-leishmanicidal-activity-of-a-biocomposite-containing-agnps-pvp-glucantime
#1
Ana Patricia Cacua Gélvez, Luis Henrique Seabra Farias, Victor Soares Pereira, Inaldo Claudio Martins da Silva, Ana Carolina Costa, Carmen Gilda Barroso Tavares Dias, Roseane Maria Ribeiro Costa, Silvia Helena Marques Da Silva, Ana Paula Drummond Rodrigues
AIM: Development of functionalized nanocomposites containing AgNPs-PVP-Glucantime® to evaluate their leishmanicidal activity as a novel method for improving the pharmacological properties of the drug Glucantime® against extracellular promastigotes and intracellular amastigotes of Leishmania amazonensis in vitro to treat cutaneous leishmaniasis. MATERIALS & METHODS: The silver nanoparticles and nanocomposites prepared containing silver nanoparticles, polyvinylpyrrolidone and different amounts of Glucantime were characterized using transmission electron microscopy, x-ray diffraction, energy-dispersive x-ray spectroscopy and ζ potential analysis; in addition, the in vitro cytotoxicity was evaluated...
January 17, 2018: Nanomedicine
https://www.readbyqxmd.com/read/29334833/silver-nanoparticles-induce-neurotoxicity-in-a-human-embryonic-stem-cell-derived-neuron-and-astrocyte-network
#2
Neza Repar, Hao Li, Jose S Aguilar, Qingshun Quinn Li, Damjana Drobne, Yiling Hong
Silver nanoparticles (AgNPs) are among the most extensively used nanoparticles and are found in a variety of products. This ubiquity leads to inevitable exposure to these particles in everyday life. However, the effects of AgNPs on neuron and astrocyte networks are still largely unknown. In this study, we used neurons and astrocytes derived from human embryonic stem cells as a cellular model to study the neurotoxicity that is induced by citrate-coated AgNPs (AgSCs). Immunostaining with the astrocyte and neuron markers, glial fibrillary acidic protein and microtubule-associated protein-2 (MAP2), respectively, showed that exposure to AgSCs at the concentration of 0...
January 15, 2018: Nanotoxicology
https://www.readbyqxmd.com/read/29334612/tempo-oxidized-bacterial-cellulose-pellicle-with-silver-nanoparticles-for-wound-dressing
#3
Chun-Nan Wu, Shih-Chang Fuh, Shin-Ping Lin, Yen-Yi Lin, Hung-Yueh Chen, Jui-Ming Liu, Kuan-Chen Cheng
Biocompatible bacterial cellulose pellicle (BCP) is a candidate for biomedical material such as wound dressing. However, due to lack of antibacterial activity, to grant BCP with the property is crucial for its biomedical application. In the present study, BCP was modified by 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation using TEMPO/NaClO/NaBr system at pH 10 to form TEMPO-oxidized BCP (TOBCP) with anionic C6 carboxylate groups. The TOBCP was subsequently ion-exchanged in AgNO3 solution and silver nanoparticles (AgNP) with diameter of ~16...
January 15, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29332827/rapid-and-sensitive-detection-of-malachite-green-in-aquaculture-water-by-electrochemical-preconcentration-and-surface-enhanced-raman-scattering
#4
Kai-Xuan Xu, Mei-Hong Guo, Yu-Ping Huang, Xiao-Dong Li, Jian-Jun Sun
A highly sensitive and rapid method of in-situ surface-enhanced Raman spectroscopy (SERS) combining with electrochemical preconcentration (EP) in detecting malachite green (MG) in aquaculture water was established. Ag nanoparticles (AgNPs) were synthesized and spread onto the surface of gold electrodes after centrifuging to produce SERS-active substrates. After optimizing the pH values, preconcentration potentials and times, in-situ EP-SERS detection was carried out. A sensitive and rapid analysis of the low-concentration MG was accomplished within 200s and the limit of detection was 2...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332821/determination-of-pyrophosphate-and-sulfate-using-polyhexamethylene-guanidine-hydrochloride-stabilized-silver-nanoparticles
#5
E A Terenteva, V V Apyari, S G Dmitrienko, A V Garshev, P A Volkov, Yu A Zolotov
Positively charged polyhexamethylene guanidine hydrochloride-stabilized silver nanoparticles (PHMG-AgNPs) were prepared and applied as a colorimetric probe for single-step determination of pyrophosphate and sulfate. The approach is based on the nanoparticles aggregation leading to change in their absorption spectra and color of the solution. Due to both electrostatic and steric stabilization these nanoparticles show decreased sensitivity relatively to many common anions, which allows for simple and rapid direct single-step determination of pyrophosphate and sulfate...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29331861/synthesis-of-colloidal-silver-nanoparticle-clusters-and-their-application-in-ascorbic-acid-detection-by-sers
#6
Jorge L Cholula-Díaz, Diana Lomelí-Marroquín, Bidhan Pramanick, Alfonso Nieto-Argüello, Luis A Cantú-Castillo, Hyundoo Hwang
Ascorbic acid (vitamin C) has an essential role in the human body mainly due to its antioxidant function. In this work, metallic silver nanoparticle (AgNP) colloids were used in SERS experiments to detect ascorbic acid in aqueous solution. The AgNPs were synthesized by a green method using potato starch as reducing and stabilizing agent, and water as the solvent. The optical properties of the yellowish as-synthesized silver colloids were characterized by UV-vis spectroscopy, in which besides a typical band at 410 nm related to the localized surface plasmon resonance of the silver nanoparticles, a shoulder band around 500 nm, due to silver nanoparticle cluster formation, is presented when relatively higher concentrations of starch are used in the synthesis...
December 29, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29331826/pathway-and-mechanism-of-nitrogen-transformation-during-composting-functional-enzymes-and-genes-under-different-concentrations-of-pvp-agnps
#7
Guangming Zeng, Lihua Zhang, Haoran Dong, Yaoning Chen, Jiachao Zhang, Yuan Zhu, Yujie Yuan, Yankai Xie, Wei Fang
Polyvinylpyrrolidone coated silver nanoparticles (PVP-AgNPs) were applied at different concentrations to reduce total nitrogen (TN) losses and the mechanisms of nitrogen bio-transformation were investigated in terms of the nitrogen functional enzymes and genes. Results showed that mineral N in pile 3 which was treated with AgNPs at a concentration of 10 mg/kg compost was the highest (6.58 g/kg dry weight (DW) compost) and the TN loss (47.07%) was the lowest at the end of composting. Correlation analysis indicated that TN loss was significantly correlated with amoA abundance...
December 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29331758/caspase-3-mapk-pathways-as-main-regulators-of-the-apoptotic-effect-of-the-phyto-mediated-synthesized-silver-nanoparticle-from-dried-stem-of-eleutherococcus-senticosus-in-human-cancer-cells
#8
Chun Gon Kim, Verónica Castro-Aceituno, Ragavendran Abbai, Hyun A Lee, Shakina Yesmin Simu, Yaxi Han, Joon Hurh, Yeon-Ju Kim, Deok Chun Yang
Siberian ginseng (Eleutherococcus senticosus) was used for the synthesis of an ecofriendly silver nanoparticle (Sg-AgNP), which has exhibited antibacterial, antioxidant effect and lower cytotoxicity to normal cells in comparison to human cancer cells. Although, the potential anticancer activity of Sg-AgNP has not been determined. In this study, two cancer cell lines were used to evaluate the cytotoxicity and apoptotic effect of Sg-AgNP along with the determination of the role of the Caspase-3 / p38 MAPK pathways...
January 9, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29331734/in-vitro-assessment-of-silver-nanoparticles-immunotoxicity
#9
Valentina Galbiati, Laura Cornaghi, Elisabetta Gianazza, Marco A Potenza, Elena Donetti, Marina Marinovich, Emanuela Corsini
This study aimed to characterize unwanted immune effects of nanoparticles (NP) using THP-1 cells, human whole blood and enriched peripheral blood monocytes. Commercially available silver NP (AgNP < 100 nm, also confirmed by Single Particle Extinction and Scattering) were used as prototypical NP. Cells were treated with AgNP alone or in combination with classical immune stimuli (i.e. LPS, PHA, PWM) and cytokine assessed; in addition, CD54 and CD86 expression was evaluated in THP-1 cells. AgNP alone induced dose-related IL-8 production in all models, with higher response observed in THP-1 cells, possibly connected to different protein corona formation in bovine versus human serum...
January 10, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29331621/silver-ion-enhanced-particle-specific-cytotoxicity-of-silver-nanoparticles-and-effect-on-the-production-of-extracellular-secretions-of-phanerochaete-chrysosporium
#10
Zhenzhen Huang, Piao Xu, Guiqiu Chen, Guangming Zeng, Anwei Chen, Zhongxian Song, Kai He, Lei Yuan, Hui Li, Liang Hu
This study investigated the influence of silver ions (Ag+) on the cytotoxicity of silver nanoparticles (AgNPs) in Phanerochaete chrysosporium and noted the degree of extracellular secretions in response to the toxicant's stress. Oxalate production was elicited with moderate concentrations of 2,4-dichlorophenol (2,4-DCP) and AgNPs reaching a plateau at 10 mg/L and 10 μM, respectively. Increased oxalate accumulation was accompanied by higher activities of manganese peroxidase (MnP) and lignin peroxidase (LiP)...
December 30, 2017: Chemosphere
https://www.readbyqxmd.com/read/29329095/investigation-of-silver-ag-deposition-in-tissues-from-stranded-cetaceans-by-autometallography-amg
#11
Wen-Ta Li, Hui-Wen Chang, Meng-Hsien Chen, Hue-Ying Chiou, Bang-Yeh Liou, Victor Fei Pang, Wei-Cheng Yang, Chian-Ren Jeng
Silver, such as silver nanoparticles (AgNPs), has been widely used in commercial products and may be released into the environment. The interaction between Ag deposition and biological systems is raising serious concerns because of one health consideration. Cetaceans, as the top predators of the oceans, may be exposed to Ag/Ag compounds and suffer negative health impacts from the deposition of these compounds in their bodies. In the present study, we utilized autometallography (AMG) to localize the Ag in the liver and kidney tissues of cetaceans and developed a model called the cetacean histological Ag assay (CHAA) to estimate the Ag concentrations in the liver and kidney tissues of cetaceans...
January 9, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29327006/spectrochemical-analyses-of-growth-phase-related-bacterial-responses-to-low-environmentally-relevant-concentrations-of-tetracycline-and-nanoparticulate-silver
#12
Naifu Jin, Kirk T Semple, Longfei Jiang, Chunling Luo, Dayi Zhang, Francis L Martin
Exposure to environmental insults generally occurs at low levels, making it challenging to measure bacterial responses to such interactions. Additionally, microbial behaviour and phenotype varies in differing bacterial types or growth phases, likely giving rise to growth- or species-specific responses to environmental stimuli. The present study applied a spectrochemical tool, infrared (IR) spectral interrogation coupled with multivariate analysis, to investigate the growth- and species-specific responses of two bacterial strains, Gram-negative Pseudomonas fluorescens and Gram-positive Mycobacterium vanbaalenii, to low concentrations of tetracycline, nanoparticulate silver (AgNP) or mixtures thereof...
January 12, 2018: Analyst
https://www.readbyqxmd.com/read/29323901/microbial-biosynthesis-of-silver-nanoparticles-in-different-culture-media
#13
Ke Luo, Samuel Jung, Kyu-Hwan Park, Young-Rok Kim
Microbial biosynthesis of metal nanoparticles has been extensively studied for the applications in biomedical sciences and engineering. However, the mechanism for their synthesis through microorganism is not completely understood. In this study, several culture media were investigated for their roles in the microbial biosynthesis of silver nanoparticles (AgNPs). The size and morphology of the synthesized AgNPs were analyzed by UV-Vis spectroscopy, FT-IR, TEM, and DLS. The results demonstrated that nutrient broth (NB) and Mueller-Hinton broth (MHB) among tested media effectively reduced silver ions to form AgNPs with different particle size and shape...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29321492/quantification-of-silver-nanoparticle-toxicity-to-algae-in-soil-via-photosynthetic-and-flow-cytometric-analyses
#14
Sun-Hwa Nam, Jin Il Kwak, Youn-Joo An
Soil algae, which have received attention for their use in a novel bioassay to evaluate soil toxicity, expand the range of terrestrial test species. However, there is no information regarding the toxicity of nanomaterials to soil algae. Thus, we evaluated the effects of silver nanoparticles (0-50 mg AgNPs/kg dry weight soil) on the soil alga Chlamydomonas reinhardtii after six days, and assessed changes in biomass, photosynthetic activity, cellular morphology, membrane permeability, esterase activity, and oxidative stress...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321463/use-of-a-smartphone-as-a-colorimetric-analyzer-in-paper-based-devices-for-sensitive-and-selective-determination-of-mercury-in-water-samples
#15
Purim Jarujamrus, Rattapol Meelapsom, Somkid Pencharee, Apinya Obma, Maliwan Amatatongchai, Nadh Ditcharoen, Sanoe Chairam, Suparb Tamuang
A smartphone application, called CAnal, was developed as a colorimetric analyzer in paper-based devices for sensitive and selective determination of mercury(II) in water samples. Measurement on the double layer of a microfluidic paper-based analytical device (μPAD) fabricated by alkyl ketene dimer (AKD)-inkjet printing technique with special design doped with unmodified silver nanoparticles (AgNPs) onto the detection zones was performed by monitoring the gray intensity in the blue channel of AgNPs, which disintegrated when exposed to mercury(II) on μPAD...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29321021/silver-pig-skin-nanocomposites-and-mesenchymal-stem-cells-suitable-antibiofilm-cellular-dressings-for-wound-healing
#16
Mario Alberto Pérez-Díaz, Phaedra Silva-Bermudez, Binisa Jiménez-López, Valentín Martínez-López, Yaaziel Melgarejo-Ramírez, Ana Brena-Molina, Clemente Ibarra, Isabel Baeza, M Esther Martínez-Pardo, M Lourdes Reyes-Frías, Erik Márquez-Gutiérrez, Cristina Velasquillo, Gabriel Martínez-Castañon, Fidel Martinez-Gutierrez, Roberto Sánchez-Sánchez
BACKGROUND: Treatment of severe or chronic skin wounds is an important challenge facing medicine and a significant health care burden. Proper wound healing is often affected by bacterial infection; where biofilm formation is one of the main risks and particularly problematic because it confers protection to microorganisms against antibiotics. One avenue to prevent bacterial colonization of wounds is the use of silver nanoparticles (AgNPs); which have proved to be effective against non-multidrug-resistant and multidrug-resistant bacteria...
January 10, 2018: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/29320928/interaction-of-caffeine-and-sulfadiazine-with-lysozyme-adsorbed-at-colloidal-metal-nanoparticle-interface-influence-on-drug-transport-ability-and-antibacterial-activity
#17
Vikash K Sonu, Imocha Rajkumar, Kaushik Bhattacharjee, S R Joshi, Sivaprasad Mitra
The modulated bioactivity of proteins immobilized on nanoparticle (NP) interfaces is of tremendous interest towards designing better therapeutic and diagnostic tools. In this work, binding behavior and the antibacterial activity of free lysozyme (LYS) as well as its non-covalent assembly with silver (Ag) and gold (Au) colloidal NPs were compared in presence of two model drugs, viz. sulfadiazine (SDZ) and caffeine (CAF). Intrinsic protein fluorescence was found to quench due to the formation drug-protein complex in case of CAF resulting a linear Stern-Volmer (SV) plot with KSV = 1...
January 11, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29320426/self-assembled-agnp-containing-nanocomposites-constructed-by-electrospinning-as-efficient-dye-photocatalyst-materials-for-wastewater-treatment
#18
Yamei Liu, Caili Hou, Tifeng Jiao, Jingwen Song, Xu Zhang, Ruirui Xing, Jingxin Zhou, Lexin Zhang, Qiuming Peng
The design and self-assembly of graphene oxide (GO)-based composite membranes have attracted enormous attention due to their wide application in nanomaterial and environmental fields. In this work, we have successfully developed a strategy to fabricate new composite membranes based on poly(vinyl alcohol)/poly(acrylic acid)/carboxyl-functionalized graphene oxide modified with silver nanoparticles (PVA/PAA/GO-COOH@AgNPs), which were prepared via thermal treatment and the electrospinning technique. Due to the strong π-π forces and strong electrostatic interactions of GO-COOH sheets, the prepared composite membranes and their lager surface areas were modified by scores of AgNPs, which demonstrated that a high-efficiency photocatalyst removed the organic dyes from the aqueous solutions...
January 10, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29317760/shape-dependent-physical-mutilation-and-lethal-effects-of-silver-nanoparticles-on-bacteria
#19
Debashish Acharya, K Malabika Singha, Piyush Pandey, Bidhan Mohanta, Jina Rajkumari, L Paikhomba Singha
In this report, spherical silver nanoparticle (AgNP-sp) and rod-shaped silver nanoparticle (AgNR) were prepared by chemical reduction method and their antibacterial activity against various Gram-positive and Gram-negative bacteria had been evaluated for their efficiency. Minimal inhibitory concentration (MIC) tests were conducted to study the antibacterial properties, and substantiated with killing kinetics of silver nanoparticles (AgNPs). The study revealed that both AgNP-sp and AgNRs are good antibacterial candidates...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29315277/trends-and-advances-in-electrochemiluminescence-nanobiosensors
#20
REVIEW
Mohammad Rizwan, Noor Faizah Mohd-Naim, Minhaz Uddin Ahmed
The rapid and increasing use of the nanomaterials (NMs), nanostructured materials (NSMs), metal nanoclusters (MNCs) or nanocomposites (NCs) in the development of electrochemiluminescence (ECL) nanobiosensors is a significant area of study for its massive potential in the practical application of nanobiosensor fabrication. Recently, NMs or NSMs (such as AuNPs, AgNPs, Fe₃O₄, CdS QDs, OMCs, graphene, CNTs and fullerenes) or MNCs (such as Au, Ag, and Pt) or NCs of both metallic and non-metallic origin are being employed for various purposes in the construction of biosensors...
January 9, 2018: Sensors
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