keyword
MENU ▼
Read by QxMD icon Read
search

Precision agriculture

keyword
https://www.readbyqxmd.com/read/28434113/precision-knockdown-of-egfr-gene-expression-using-radio-frequency-electromagnetic-energy
#1
Ilya V Ulasov, Haidn Foster, Mike Butters, Jae-Geun Yoon, Tomoko Ozawa, Theodore Nicolaides, Xavier Figueroa, Parvinder Hothi, Michael Prados, John Butters, Charles Cobbs
Electromagnetic fields (EMF) in the radio frequency energy (RFE) range can affect cells at the molecular level. Here we report a technology that can record the specific RFE signal of a given molecule, in this case the siRNA of epidermal growth factor receptor (EGFR). We demonstrate that cells exposed to this EGFR siRNA RFE signal have a 30-70% reduction of EGFR mRNA expression and ~60% reduction in EGFR protein expression vs. control treated cells. Specificity for EGFR siRNA effect was confirmed via RNA microarray and antibody dot blot array...
April 22, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28430356/crispr-cas9-an-rna-guided-highly-precise-synthetic-tool-for-plant-genome-editing
#2
REVIEW
Yeliz Demirci, Baohong Zhang, Turgay Unver
CRISPR/Cas 9 is a newly developed and naturally occurred genome editing tool, which is originally used by bacteria for immune defence. In the past years, it has been quickly employed and modified to precisely edit genome sequences in both plants and animals. Compared with the well-developed zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), CRISPR/Cas 9 has lots of advantages, including easier to design and implement, higher targeting efficiency, and less expensive. Thus, it is becoming one of the most powerful tools for knockout of an individual gene as well as insertion of one gene and/or control of gene transcription...
April 21, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28426952/plant-viruses-and-bacteriophages-for-delivery-in-medicine-and-biotechnology
#3
REVIEW
Anna E Czapar, Nicole F Steinmetz
There are a wide variety of synthetic and naturally occurring nanomaterials under development for nanoscale cargo-delivery applications. Viruses play a special role in these developments, because they can be regarded as naturally occurring nanomaterials evolved to package and deliver cargos. While any nanomaterial has its advantage and disadvantages, viral nanoparticles (VNPs), in particular the ones derived from plant viruses and bacteriophages, are attractive options for cargo-delivery as they are biocompatible, biodegradable, and non-infectious to mammals...
April 17, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28424714/relationships-between-root-pathogen-resistance-abundance-and-expression-of-pseudomonas-antimicrobial-genes-and-soil-properties-in-representative-swiss-agricultural-soils
#4
Nicola Imperiali, Francesca Dennert, Jana Schneider, Titouan Laessle, Christelle Velatta, Marie Fesselet, Michele Wyler, Fabio Mascher, Olga Mavrodi, Dmitri Mavrodi, Monika Maurhofer, Christoph Keel
Strains of Pseudomonas that produce antimicrobial metabolites and control soilborne plant diseases have often been isolated from soils defined as disease-suppressive, i.e., soils, in which specific plant pathogens are present, but plants show no or reduced disease symptoms. Moreover, it is assumed that pseudomonads producing antimicrobial compounds such as 2,4-diacetylphloroglucinol (DAPG) or phenazines (PHZ) contribute to the specific disease resistance of suppressive soils. However, pseudomonads producing antimicrobial metabolites are also present in soils that are conducive to disease...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28414338/open-path-spectroscopic-methane-detection-using-a-broadband-monolithic-distributed-feedback-quantum-cascade-laser-array
#5
Anna P M Michel, Jason Kapit, Mark F Witinski, Romain Blanchard
Methane is a powerful greenhouse gas that has both natural and anthropogenic sources. The ability to measure methane using an integrated path length approach such as an open/long-path length sensor would be beneficial in several environments for examining anthropogenic and natural sources, including tundra landscapes, rivers, lakes, landfills, estuaries, fracking sites, pipelines, and agricultural sites. Here a broadband monolithic distributed feedback-quantum cascade laser array was utilized as the source for an open-path methane sensor...
April 10, 2017: Applied Optics
https://www.readbyqxmd.com/read/28412527/aquaporins-as-potential-drought-tolerance-inducing-proteins-toward-instigating-stress-tolerance
#6
Sajad Majeed Zargar, Preeti Nagar, Rupesh Deshmukh, Muslima Nazir, Aijaz Ahmad Wani, Khalid Zaffar Masoodi, Ganesh Kumar Agrawal, Randeep Rakwal
Aquaporins (AQPs) are primarily involved in maintaining cellular water homeostasis. Their role in diverse physiological processes has fascinated plant scientists for more than a decade, particularly concerning abiotic stresses. Increasing examples of evidence in various crop plants indicate that the AQPs are responsible for precise regulation of water movement and consequently play a crucial role in the drought stress tolerance. Since drought is one of the major abiotic stresses affecting agricultural production worldwide, it has become a critical agenda to focus research on the development of drought tolerant crop plants...
April 13, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28402209/culturomics-a-new-kid-on-the-block-of-omics-to-enable-personalized-medicine
#7
Manousos E Kambouris, Cristiana Pavlidis, Efthymios Skoufas, Michael Arabatzis, Maria Kantzanou, Aristea Velegraki, George P Patrinos
This innovation analysis highlights the underestimated and versatile potential of the new field of culturomics and examines its relation to other OMICS system sciences such as infectiomics, metabolomics, phenomics, and pharmacomicrobiomics. The advent of molecular biology, followed by the emergence of various disciplines of the genomics, and most importantly metagenomics, brought about the sharp decline of conventional microbiology methods. Emergence of culturomics has a natural synergy with therapeutic and clinical genomic approaches so as to realize personalized medicine...
April 12, 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28398839/foundational-and-translational-research-opportunities-to-improve-plant-health
#8
Richard W Michelmore, Gitta Coaker, Rebecca Bart, Gwyn A Beattie, Andrew Bent, Toby Bruce, Duncan Cameron, Jeff Dangl, Savithramma Dinesh-Kumar, Robert Edwards, Sebastian Eves-van den Akker, Walter Gassmann, Jean Greenberg, Richard Harrison, Ping He, Jagger Harvey, Alisa Huffaker, Scot Hulbert, Roger Innes, Jonathan D Jones, Isgouhi Kaloshian, Sophien Kamoun, Fumiaki Katagiri, Jan E Leach, Wenbo Ma, John M McDowell, June Medford, Blake Meyers, Rebecca Nelson, Richard Peter Oliver, Yiping Qi, Diane Saunders, Michael Shaw, Prasanta Subudhi, Leslie Torrance, Brett M Tyler, John Walsh
This whitepaper reports the deliberations of a workshop focused on biotic challenges to plant health held in Washington, D.C. in September 2016. Ensuring health of food plants is critical to maintaining the quality and productivity of crops and for sustenance of the rapidly growing human population. There is a close linkage between food security and societal stability; however, global food security is threatened by the vulnerability of our agricultural systems to numerous pests, pathogens, weeds, and environmental stresses...
April 11, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28386301/new-traits-in-crops-produced-by-genome-editing-techniques-based-on-deletions
#9
REVIEW
C C M van de Wiel, J G Schaart, L A P Lotz, M J M Smulders
One of the most promising New Plant Breeding Techniques is genome editing (also called gene editing) with the help of a programmable site-directed nuclease (SDN). In this review, we focus on SDN-1, which is the generation of small deletions or insertions (indels) at a precisely defined location in the genome with zinc finger nucleases (ZFN), TALENs, or CRISPR-Cas9. The programmable nuclease is used to induce a double-strand break in the DNA, while the repair is left to the plant cell itself, and mistakes are introduced, while the cell is repairing the double-strand break using the relatively error-prone NHEJ pathway...
2017: Plant Biotechnology Reports
https://www.readbyqxmd.com/read/28380618/temporal-spatial-inter-and-intra-cow-repeatability-of-thermal-imaging
#10
D T Byrne, D P Berry, H Esmonde, N McHugh
The objective of the present study was to quantify the within- and between-cow, operator, and day variances of various descriptive temperature parameters from different anatomical areas captured using thermal images on Holstein-Friesian cows. Three experiments were undertaken. In Exp. 1, 30 images were captured by a single operator of each of the eye, hoof, and udder from each of 45 cows; in Exp. 2, three different operators captured eye and hoof images from 12 cows; and in Exp. 3, eye and hoof images were captured by a single operator from 8 cows over a 5-d period...
February 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28380599/estimation-of-the-energy-expenditure-of-grazing-ruminants-by-incorporating-dynamic-body-acceleration-into-a-conventional-energy-requirement-system
#11
M Miwa, K Oishi, H Anzai, H Kumagai, S Ieiri, H Hirooka
The estimation of energy expenditure (EE) of grazing animals is of great importance for efficient animal management on pasture. In the present study, a method is proposed to estimate EE in grazing animals based on measurements of body acceleration of animals in combination with the conventional Agricultural and Food Research Council (AFRC) energy requirement system. Three-dimensional body acceleration and heart rate were recorded for tested animals under both grazing and housing management. An acceleration index, vectorial dynamic body acceleration (VeDBA), was used to calculate activity allowance (AC) during grazing and then incorporate it into the AFRC system to estimate the EE (EE derived from VeDBA [EE]) of the grazing animals...
February 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28376431/validation-of-a-quechers-method-for-analysis-of-neonicotinoids-in-small-volumes-of-blood-and-assessment-of-exposure-in-eurasian-eagle-owl-bubo-bubo-nestlings
#12
A Taliansky-Chamudis, P Gómez-Ramírez, M León-Ortega, A J García-Fernández
Neonicotinoid pesticides have gained great interest in the last years both for agricultural and domestic use. Since the information on their environmental distribution or the effects derived from exposure to ecosystems and biota is scarce, new analytical techniques are being developed for monitoring studies. In this sense, two extraction techniques based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) methodology to detect the neonicotinoids authorised in Spain (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, nitenpyram and thiamethoxam) were adapted and compared: a) using acetate buffer (AB); and b) using citrate buffer (CB)...
April 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28371222/new-variants-of-crispr-rna-guided-genome-editing-enzymes
#13
REVIEW
Jana Murovec, Žan Pirc, Bing Yang
CRISPR-mediated genome editing using the Streptococcus pyogenes Cas9 enzyme is revolutionizing life science by providing new, precise, facile and high throughput tools for genetic modification by the specific targeting of double-strand breaks in the genome of hosts. Plant biotechnologists have extensively used the S. pyogenes Cas9-based system since its inception in 2013. However, there are still some limitations to its even broader usage in plants. Major restrictions, especially in agricultural biotechnology, are the currently unclear regulatory status of plants modified with CRISPR/Cas9 and the lack of suitable delivery methods for some plant species...
April 1, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28360425/rapid-breeding-of-parthenocarpic-tomato-plants-using-crispr-cas9
#14
Risa Ueta, Chihiro Abe, Takahito Watanabe, Shigeo S Sugano, Ryosuke Ishihara, Hiroshi Ezura, Yuriko Osakabe, Keishi Osakabe
Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into SlIAA9-a key gene controlling parthenocarpy-with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome...
March 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28352989/electric-fences-and-accidental-death
#15
Michael Burke, Morris Odell, Heinrich Bouwer, Adam Murdoch
Deaths which occur in association with agricultural electric fences are very rare. In fact, electric fences have undoubtedly saved numerous human and animal lives by safely and reliably keeping livestock confined to their fields and enclosures and thus preventing motor vehicle incidents when livestock get onto roads and highways. Accidental and intentional human contact with electric fences occurs regularly and causes little more than transient discomfort, however, on exceptional occasions, contact with electric fences appears to be directly related to the death of the individual...
March 28, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28351643/chemically-modified-halloysite-nanotubes-as-a-solid-phase-microextraction-coating
#16
Mohammad Saraji, Mohammad Taghi Jafari, Mehdi Mossaddegh
Halloysite nanotubes were modified in three simple steps including etching, hydroxylation and amino grafting. The sol-gel technique was used for the chemical bonding of the modified halloysite nanotubes (MHNTs) to fused-silica support. The MHNTs, as a novel adsorbent was applied as a SPME coating. Diazinon, parathion and fenthion were selected as the model compounds to study the extraction efficiency of the coating. Gas chromatography-corona discharge ion mobility spectrometry was applied for the analysis of the extracted analytes...
April 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28346499/a-farm-level-precision-land-management-framework-based-on-integer-programming
#17
Qi Li, Guiping Hu, Talukder Zaki Jubery, Baskar Ganapathysubramanian
Farmland management involves several planning and decision making tasks including seed selection and irrigation management. A farm-level precision farmland management model based on mixed integer linear programming is proposed in this study. Optimal decisions are designed for pre-season planning of crops and irrigation water allocation. The model captures the effect of size and shape of decision scale as well as special irrigation patterns. The authors illustrate the model with a case study on a farm in the state of California in the U...
2017: PloS One
https://www.readbyqxmd.com/read/28345909/rapid-and-simultaneous-analysis-of-360-pesticides-in-brown-rice-spinach-orange-and-potato-using-microbore-gc-ms-ms
#18
Jonghwa Lee, Leesun Kim, Yongho Shin, Junghak Lee, Jiho Lee, Eunhye Kim, Joon-Kwan Moon, Jeong-Han Kim
A multiresidue method for the simultaneous and rapid analysis of 360 pesticides in representative agricultural produce (brown rice, orange, spinach, and potato) was developed using a modified QuEChERS procedure combined with gas chromatography-tandem mass spectrometry (GC-MS/MS). Selected reaction monitoring transition parameters (e.g., collision energy, precursor and product ions) in MS/MS were optimized to achieve the best selectivity and sensitivity for a wide range of GC-amenable pesticides. A short (20 m) microbore (0...
April 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28345051/aflatoxin-free-transgenic-maize-using-host-induced-gene-silencing
#19
Dhiraj Thakare, Jianwei Zhang, Rod A Wing, Peter J Cotty, Monica A Schmidt
Aflatoxins, toxic secondary metabolites produced by some Aspergillus species, are a universal agricultural economic problem and a critical health issue. Despite decades of control efforts, aflatoxin contamination is responsible for a global loss of millions of tons of crops each year. We show that host-induced gene silencing is an effective method for eliminating this toxin in transgenic maize. We transformed maize plants with a kernel-specific RNA interference (RNAi) gene cassette targeting the aflC gene, which encodes an enzyme in the Aspergillus aflatoxin biosynthetic pathway...
March 2017: Science Advances
https://www.readbyqxmd.com/read/28342419/incorporating-denitrification-decomposition-method-to-estimate-field-emissions-for-life-cycle-assessment
#20
Yelin Deng, Dimos Paraskevas, Shi-Jie Cao
This study focuses on a detailed Life Cycle Assessment (LCA) for flax cultivation in Northern France. Nitrogen related field emissions are derived both from a process-oriented DeNitrification-DeComposition (DNDC) method and the generic Intergovernmental Panel on Climate Change (IPCC) method. Since the IPCC method is synthesised from field measurements at sites with various soil types, climate conditions, and crops, it contains significant uncertainties. In contrast, the outputs from the DNDC method are considered as more site specific as it is built according to complex models of soil science...
March 22, 2017: Science of the Total Environment
keyword
keyword
24724
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"