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Polysaccharide klebsiella

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https://www.readbyqxmd.com/read/29305504/an-igaa-umob-family-protein-from-serratia-marcescens-regulates-motility-capsular-polysaccharide-and-secondary-metabolite-production
#1
Nicholas A Stella, Kimberly M Brothers, Jake D Callaghan, Angelina M Passerini, Cihad Sigindere, Preston J Hill, Xinyu Liu, Daniel J Wozniak, Robert M Q Shanks
Secondary metabolites are an important source of pharmaceuticals and key modulators of microbe-microbe interactions. The bacterium Serratia marcescens is part of the Enterobacteriaceae family of eubacteria and produces a number of biologically active secondary metabolites. In this study, we screened for novel regulators of secondary metabolites synthesized by a clinical isolate of S. marcescens and found mutations in a gene for an uncharacterized UmoB/IgaA family member here named gumB Mutation of gumB conferred a severe loss of secondary metabolites prodigiosin and serratamolide...
January 5, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29297821/isolation-identification-and-genome-analysis-of-lung-pathogenic-klebsiella-pneumoniae-lpkp-in-forest-musk-deer
#2
Wei Zhao, Qing Tian, Yan Luo, Yin Wang, Ze-Xiao Yang, Xue-Ping Yao, Jian-Guo Cheng, Xin Zhou, Wu-You Wang
Klebsiella pneumoniae is an important pathogen commonly associated with opportunistic infections. In this study, lung pathogenic K. pneumoniae (LPKP) was isolated and identified from suppurative pneumoniae in forest musk deer by conventional methods and by 16S ribosomal RNA sequence analysis. Median lethal dose and histopathologic analysis were used to demonstrate pathogenicity of the organism in mice. Furthermore, a draft genome of LPKP was sequenced, and its virulence genes were detected. One hundred and twenty-two virulence genes encoded determinant of capsule polysaccharide (CPS), lipopolysaccharide, fimbriae, outer membrane proteins, iron acquisition, and urease...
December 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/29273737/hydrolytic-activity-determination-of-tail-tubular-protein-a-of-klebsiella-pneumoniae-bacteriophages-towards-saccharide-substrates
#3
Ewa Brzozowska, Anna Pyra, Krzysztof Pawlik, Monika Janik, Sabina Górska, Natalia Urbańska, Zuzanna Drulis-Kawa, Andrzej Gamian
In this paper, the enzymatic activity, substrate specificity and antibiofilm feature of bacteriophage dual-function tail proteins are presented. So far, tail tubular proteins A-TTPAgp31 and TTPAgp44-have been considered as structural proteins of Klebsiella pneumoniae bacteriophages KP32 and KP34, respectively. Our results show that TTPAgp31 is able to hydrolyze maltose as well as Red-starch. The activity of 1 µM of the protein was calculated as 47.6 milli-Units/assay relating to the α-amylase activity. It degrades capsular polysaccharides (cPS), slime polysaccharides (sPS) and lipopolysaccharide (LPS) of K...
December 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29209298/the-gut-entomotype-of-red-palm-weevil-rhynchophorus-ferrugineus-olivier-coleoptera-dryophthoridae-and-their-effect-on-host-nutrition-metabolism
#4
Abrar Muhammad, Ya Fang, Youming Hou, Zhanghong Shi
For invasive insects, the potential roles of gut microbiota in exploiting new food resources and spreading remain elusive. Red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, is an invasive destructive pest which feeds on nutrient-poor tender tissues and has caused extensive mortality of palm trees. The microbes associated with insects can improve their nutrition assimilation. However, experimental evidence on the interactions between RPW and its gut microbiota is still absent. The aim of this study is to determine the dynamics changes and the bacterial entomotype in the RPW gut and its potential physiological roles...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29186063/structural-masquerade-of-plesiomonas-shigelloides-strain-cnctc-78-89-o-antigen-high-resolution-magic-angle-spinning-nmr-reveals-the-modified-d-galactan-i-of-klebsiella-pneumoniae
#5
Karolina Ucieklak, Sabina Koj, Damian Pawelczyk, Tomasz Niedziela
The high-resolution magic angle spinning nuclear magnetic resonance spectroscopy (HR-MAS NMR) analysis of Plesiomonas shigelloides 78/89 lipopolysaccharide directly on bacteria revealed the characteristic structural features of the O-acetylated polysaccharide in the NMR spectra. The O-antigen profiles were unique, yet the pattern of signals in the, spectra along with their ¹H,13C chemical shift values, resembled these of d-galactan I of Klebsiella pneumoniae. The isolated O-specific polysaccharide (O-PS) of P...
November 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29153063/-investigation-of-various-virulence-factors-of-klebsiella-pneumoniae-strains-isolated-from-nosocomial-infections
#6
Halit Kuş, Uğur Arslan, Hatice Türk Dağı, Duygu Fındık
Klebsiella pneumoniae is an opportunistic pathogen that commonly affects immunosuppressed patients and causes nosocomial infections. K.pneumoniae has a variety of virulence factors, especially capsule polysaccharide, hypermucoviscosity (HV), fimbriae, toxins and determinants for iron acquisition. The aim of this study was to detect the virulence factors in K.pneumoniae strains isolated from nosocomial infections in two years. Fifty three K.pneumoniae strains isolated from the samples of patients with nosocomial infections in the Medical Microbiology Laboratory of Selcuk University Faculty of Medicine Hospital between 2011 and 2013 were included in the study...
October 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28988127/comparative-genome-analysis-of-novel-podoviruses-lytic-for-hypermucoviscous-klebsiella-pneumoniae-of-k1-k2-and-k57-capsular-types
#7
Ekaterina V Solovieva, Vera P Myakinina, Angelina A Kislichkina, Valentina M Krasilnikova, Vladimir V Verevkin, Vladimir V Mochalov, Anastasia I Lev, Nadezhda K Fursova, Nikolay V Volozhantsev
Hypermucoviscous (HV) strains of capsular types K1, K2 and K57 are the most virulent representatives of the Klebsiella pneumoniae species. Eight novel bacteriophages lytic for HV K. pneumoniae were isolated and characterized. Three bacteriophages, KpV41, KpV475, and KpV71 were found to have a lytic activity against mainly K. pneumoniae of capsular type K1. Two phages, KpV74, and KpV763 were lytic for K2 capsular type K. pneumoniae, and the phage KpV767 was specific to K57-type K. pneumoniae only. Two more phages, KpV766, and KpV48 had no capsular specificity...
October 5, 2017: Virus Research
https://www.readbyqxmd.com/read/28877651/production-of-a-bioflocculant-from-klebsiella-sp-os-1-using-brewery-wastewater-as-a-source
#8
Lili Ma, Jingjing Liang, Shanyi Wang, Bing Yang, Mingyan Chen, Yucheng Liu
Abstracts This study evaluated the potential of bioflocculant production from a Klebsiella strain using brewery wastewater as nutrients. The bioflocculant named OS-1B produced by Klebsiella sp. OS-1 exhibited a good flocculating activity to kaolin clay suspension (around 95%), when the diluted brewery wastewater with 7.2 mg/L total nitrogen and 1013 mg/L CODCr, was used as a nitrogen source. Glucose (15 g/L) is the most favorable carbon source for Klebsiella sp. OS-1 in bioflocculant production from the brewery wastewater...
September 7, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28815890/a-semi-synthetic-glycoconjugate-vaccine-candidate-for-carbapenem-resistant-klebsiella-pneumoniae
#9
Peter H Seeberger, Claney L Pereira, Naeem Khan, Guozhi Xiao, Elizabeth Diago-Navarro, Katrin Reppe, Bastian Opitz, Bettina C Fries, Martin Witzenrath
Hospital-acquired infections are an increasingly serious health concern. Infections caused by carpabenem-resistant Klebsiella pneumoniae (CR-Kp) are especially problematic, with a 50 % average survival rate. CR-Kp are isolated from patients with ever greater frequency, 7 % within the EU but 62 % in Greece. At a time when antibiotics are becoming less effective, no vaccines to protect from this severe bacterial infection exist. Herein, we describe the convergent [3+3] synthesis of the hexasaccharide repeating unit from its capsular polysaccharide and related sequences...
August 16, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28743631/molecular-characterization-and-functional-analysis-of-the-hepcidin-gene-from-roughskin-sculpin-trachidermus-fasciatus
#10
Yingying Liu, Xiaodi Han, Xuezhao Chen, Shanshan Yu, Yingmei Chai, Tongjie Zhai, Qian Zhu
Hepcidin is a kind of cysteine-rich antimicrobial peptide that plays a vital role in host innate immune activity and iron regulation. Here, we report the molecular characterization and functional analysis of a novel hamp1 hepcidin isoforms Tf-Hep from roughskin sculpin, Trachidermus fasciatus. A cDNA fragment of 988 bp with an ORF of 273 bp was obtained. The coding sequence encodes for a signal peptide of 24 amino acids coupled with a prodomain of 40 amino acids and a mature peptide of 26 amino acids. Tissue distribution analysis indicated that Tf-Hep was most abundant in the liver...
July 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28731269/biofilm-formation-and-antibiotic-resistance-in-klebsiella-pneumoniae-urinary-strains
#11
C Vuotto, F Longo, C Pascolini, G Donelli, M P Balice, M F Libori, V Tiracchia, A Salvia, P E Varaldo
AIMS: Multidrug-resistant Klebsiella pneumoniae has become a relevant healthcare-associated pathogen. Capsule, type 1 and 3 fimbriae (mrkA gene), type 2 quorum-sensing system (luxS), synthesis of D-galactan I (wbbM), LPS transport (wzm) and poly-beta-1,6-N-acetyl-D-glucosamine (pgaA) seem involved in K. pneumoniae biofilm. Nonenzymatic antibiotic resistance is related to nonexpression or mutation of porins (OmpK35 and OmpK36), and efflux pump (acrB) overexpression. The aim of this study was to analyse some virulence factors of K...
July 21, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28729207/first-two-cases-of-severe-multifocal-infections-caused-by-klebsiella-pneumoniae-in-switzerland-characterization-of-an-atypical-non-k1-k2-serotype-strain-causing-liver-abscess-and-endocarditis
#12
Baharak Babouee Flury, Valentina Donà, Niccolò Buetti, Hansjakob Furrer, Andrea Endimiani
BACKGROUND: We describe the first two multifocal invasive infections due to Klebsiella pneumoniae recently observed in Switzerland. METHODS: Phenotypic (MIC assays and string test) and molecular analyses (PCR/Sequencing for bla, virulence factor genes and whole genome sequencing for one strain) were performed to characterize the causative K. pneumoniae isolates. RESULTS: Both K. pneumoniae isolates (Kp1 and Kp2) were pan-susceptible to antibiotics and produced narrow-spectrum SHV β-lactamases...
September 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28728189/spontaneous-globe-rupture-due-to-rapidly-evolving-endogenous-hypermucoid-klebsiella-pneumoniae-endophthalmitis
#13
Edward H Wood, Matthew A Powers, Darius M Moshfeghi
In this retrospective report, the authors describe a rare case of spontaneous globe rupture from Klebsiella pneumoniae endophthalmitis in a middle-aged man with poorly controlled type 2 diabetes mellitus. There have been only four previously reported cases of spontaneous globe rupture from endophthalmitis. Out of the now five reported cases, all have been due to endogenous endophthalmitis, four have been due to gram-negative bacteria, and three have been due to K. pneumoniae. K. pneumoniae, especially the hypermucoid variant with a protective polysaccharide capsule, is an emerging pathogen with remarkable virulence...
July 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28716362/phosphoenolpyruvate-phosphotransferase-system-components-positively-regulate-klebsiella-biofilm-formation
#14
Yu-Tze Horng, Chi-Jen Wang, Wen-Ting Chung, Huei-Jen Chao, Yih-Yuan Chen, Po-Chi Soo
BACKGROUND/PURPOSE: Klebsiella pneumoniae is one of the leading causes of device-related infections (DRIs), which are associated with attachment of bacteria to these devices to form a biofilm. The latter is composed of not only bacteria but also extracellular polymeric substances (EPSes) consisting of extracellular DNAs, polysaccharides, and other macromolecules. The phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) regulates diverse processes of bacterial physiology...
June 29, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28693930/the-prevalence-of-rectal-carriage-of-klebsiella-pneumoniae-amongst-diabetic-patients-and-their-clinical-relevance-in-taiwan-a-five-year-prospective-study
#15
Yu-Tsung Huang, Ju-Ying Jiang, Meng-Shiuan Hsu, Hsin-Sui Hsu, Chun-Hsing Liao, Po-Ren Hsueh
BACKGROUND/PURPOSE: Pyogenic liver abscess (PLA) and bacteremia caused by Klebsiella pneumoniae is a common complication among patients with diabetes mellitus (DM). The aim of this study is to investigate the prevalence of rectal carriage and serotype distribution of K. pneumoniae amongst DM patients and their clinical relevance. METHODS: We prospectively collected rectal swabs for K. pneumoniae culture in asymptomatic DM patients from March 2008 to June 2009. Seven capsular serotypes that were commonly associated with PLA were determined by capsular polysaccharide synthesis (cps) genotyping...
June 29, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28676686/two-t7-like-bacteriophages-k5-2-and-k5-4-each-encodes-two-capsule-depolymerases-isolation-and-functional-characterization
#16
Pei-Fang Hsieh, Hsiao-Hsuan Lin, Tzu-Lung Lin, Yi-Yin Chen, Jin-Town Wang
Two Klebsiella bacteriophages K5-2 and K5-4, which are able to infect and grow on either capsular types K30/K69 and K5 or K8 and K5 of Klebsiella strains, were isolated and characterized. Each phage contained two open reading frames (ORFs), which encoded two putative capsule depolymerases, respectively. The first ORF encoded tail fiber proteins, which have K30/K69 depolymerase and K8 depolymerase activities. The second ORF encoded hypothetical proteins, which are almost identical in amino acid sequences, and have K5 depolymerase activity...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28617330/identification-of-pathogenic-factors-in-klebsiella-pneumoniae-using-impedimetric-sensor-equipped-with-biomimetic-surfaces
#17
Duyen Thi Ngoc Huynh, Ah-Young Kim, Young-Rok Kim
K.pneumoniae is an opportunistic pathogen that causes nosocomial infections, such as, pneumonia, urinary tract infections, septic shock, and gastro intestinal disease. Lipopolysaccharide (LPS), capsular polysaccharide, and fimbriae are recognized major virulence factors of K.pneumoniae and play key roles during early stages of infections. In this study, we functionalized the surface of gold electrode with mannose and mucin to monitor the adhesion-associated virulence factors of K.pneumoniae. The binding characteristics of K...
June 15, 2017: Sensors
https://www.readbyqxmd.com/read/28599670/whole-genome-sequencing-of-an-expec-that-caused-fatal-pneumonia-at-a-pig-farm-in-changchun-china
#18
Ling-Cong Kong, Xia Guo, Zi Wang, Yun-Hang Gao, Bo-Yan Jia, Shu-Ming Liu, Hong-Xia Ma
BACKGROUND: In recent years, highly frequent swine respiratory diseases have been caused by extraintestinal pathogenic Escherichia coli (ExPEC) in China. Due to this increase in ExPECs, this bacterial pathogen has become a threat to the development of the Chinese swine industry. To investigate ExPEC pathogenesis, we isolated a strain (named SLPE) from lesioned porcine lungs from Changchun in China, reported the draft genome and performed comparative genomic analyses. RESULTS: Based on the gross post-mortem examination, bacterial isolation, animal regression test and 16S rRNA gene sequence analysis, the pathogenic bacteria was identified as an ExPEC...
June 9, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28586386/differential-th17-response-induced-by-the-two-clades-of-the-pandemic-st258-klebsiella-pneumoniae-clonal-lineages-producing-kpc-type-carbapenemase
#19
Ann Maria Clemente, Giuseppe Castronovo, Alberto Antonelli, Marco Maria D'Andrea, Michele Tanturli, Eloisa Perissi, Sara Paccosi, Astrid Parenti, Federico Cozzolino, Gian Maria Rossolini, Maria Gabriella Torcia
The spread of KPC-type carbapenemases is mainly attributed to the global dissemination of Klebsiella pneumoniae (KP) strains belonging to the clonal group (CG) 258, including sequence type (ST) 258 and other related STs. Two distinct clades of CG258-KP have evolved, which differ mainly for the composition of their capsular polysaccharides, and recent studies indicate that clade 1 evolved from an ancestor of clade 2 by recombination of a genomic fragment carrying the capsular polysaccharide (cps) locus. In this paper, we investigated the ability of two ST258-KP strains, KKBO-1 and KK207-1, selected as representatives of ST258-KP clade 2 and clade 1, respectively, to activate an adaptive immune response using ex vivo-stimulation of PBMC from normal donors as an experimental model...
2017: PloS One
https://www.readbyqxmd.com/read/28578961/novel-nano-particulated-exopolysaccharide-produced-by-klebsiella-sp-phrc1-001
#20
Meng Zhao, Nana Cui, Fangning Qu, Xue Huang, Hao Yang, Shaoping Nie, Xueqiang Zha, Steve W Cui, Katsuyoshi Nishinari, Glyn O Phillips, Yapeng Fang
In recent decades, microbial synthesis of polysaccharides with special functional properties has attracted increasing attention. This work reported a novel exopolysaccharide (EPS)-producing strain, Klebsiella sp. PHRC1.001 isolated from activated sludge. Physicochemical, rheological, emulsifying and toxicological properties of the obtained EPS were characterized. The EPS was mainly composed of d-glucose and l-arabinose, and was found to exist in aqueous solution in a nano-particulated form (∼50nm in diameter) with a strong tendency of aggregation...
September 1, 2017: Carbohydrate Polymers
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