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Polysaccharide klebsiella

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https://www.readbyqxmd.com/read/29783055/characterization-and-coagulation-flocculation-performance-of-a-composite-flocculant-in-high-turbidity-drinking-water-treatment
#1
Xiang Xia, Shuhuan Lan, Xudong Li, Yifei Xie, Yajie Liang, Peihan Yan, Zhengyang Chen, Yunxiao Xing
Klebsiella variicola B16, a microbial bioflocculant (MBF-B16)-producing bacteria, was isolated and identified by its 16S rRNA sequence, biochemical properties, and physiological characteristics. The effects of culture conditions on MBF-B16 production, including carbon source, nitrogen source, C/N ratio, initial pH, and culture temperature, were investigated in this study. Results showed that 6.96 g of MBF-B16 could be extracted from a 1-L culture broth under optimized conditions. Chemical analysis showed that polysaccharide and protein were the main components...
April 30, 2018: Chemosphere
https://www.readbyqxmd.com/read/29764953/fighting-antibiotic-resistant-klebsiella-pneumoniae-with-sweet-immune-targets
#2
Roberto Adamo, Immaculada Margarit
Antibiotics and vaccines have greatly impacted human health in the last century by dramatically reducing the morbidity and mortality associated with infectious diseases. The recent challenge posed by the emergence of multidrug-resistant bacteria could possibly be addressed by novel immune prophylactic and therapeutic approaches. Among the newly threatening pathogens, Klebsiella pneumoniae is particularly worrisome in the nosocomial setting, and its surface polysaccharides are regarded as promising antigen candidates...
May 15, 2018: MBio
https://www.readbyqxmd.com/read/29760533/cross-specificity-of-protective-human-antibodies-against-klebsiella-pneumoniae-lps-o-antigen
#3
Tim Rollenske, Valeria Szijarto, Jolanta Lukasiewicz, Luis M Guachalla, Katarina Stojkovic, Katharina Hartl, Lukas Stulik, Simone Kocher, Felix Lasitschka, Mohammed Al-Saeedi, Jutta Schröder-Braunstein, Moritz von Frankenberg, Gereon Gaebelein, Peter Hoffmann, Sabrina Klein, Klaus Heeg, Eszter Nagy, Gabor Nagy, Hedda Wardemann
Humoral immune responses to microbial polysaccharide surface antigens can prevent bacterial infection but are typically strain specific and fail to mediate broad protection against different serotypes. Here we describe a panel of affinity-matured monoclonal human antibodies from peripheral blood immunoglobulin M-positive (IgM+ ) and IgA+ memory B cells and clonally related intestinal plasmablasts, directed against the lipopolysaccharide (LPS) O-antigen of Klebsiella pneumoniae, an opportunistic pathogen and major cause of antibiotic-resistant nosocomial infections...
May 14, 2018: Nature Immunology
https://www.readbyqxmd.com/read/29724185/clonal-diversity-and-genetic-profiling-of-antibiotic-resistance-among-multidrug-carbapenem-resistant-klebsiella-pneumoniae-isolates-from-a-tertiary-care-hospital-in-saudi-arabia
#4
Taher Uz Zaman, Maha Alrodayyan, Maha Albladi, Mohammed Aldrees, Mohammed Ismail Siddique, Sameera Aljohani, Hanan H Balkhy
BACKGROUND: The nexus between resistance determinants, plasmid type, and clonality appears to play a crucial role in the dissemination and survival of carbapenem-resistant Klebsiella pneumoniae (CRKP). The incidence of infections involving CRKP in Saudi Arabia is increasing and there is a need for detailed molecular profiling of this pathogen for CRKP surveillance and control. METHODS: The resistance determinants of 71 non-redundant CRKP isolates were investigated by polymerase chain reaction (PCR) and sequencing...
May 3, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29618504/kaptive-web-user-friendly-capsule-and-lipopolysaccharide-serotype-prediction-for-klebsiella-genomes
#5
Ryan R Wick, Eva Heinz, Kathryn E Holt, Kelly L Wyres
As whole genome sequencing becomes an established component of the microbiologist's toolbox, it is imperative that researchers, clinical microbiologists and public health professionals have access to genomic analysis tools for rapid extraction of epidemiologically and clinically relevant information. For the gram-negative hospital pathogens such as Klebsiella pneumoniae , initial efforts have focused on detection and surveillance of antimicrobial resistance genes and clones. However, with the resurgence of interest in alternative infection control strategies targeting Klebsiella surface polysaccharides, the ability to extract information about these antigens is increasingly important...
April 4, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29617698/pseudomonas-aeruginosa-and-klebsiella-pneumoniae-adaptation-to-innate-immune-clearance-mechanisms-in-the-lung
#6
Sebastian A Riquelme, Danielle Ahn, Alice Prince
Many different species of gram-negative bacteria are associated with infection in the lung, causing exacerbations of chronic obstructive pulmonary disease, cystic fibrosis (CF), and ventilator-associated pneumonias. These airway pathogens must adapt to common host clearance mechanisms that include killing by antimicrobial peptides, antibiotics, oxidative stress, and phagocytosis by leukocytes. Bacterial adaptation to the host is often evident phenotypically, with increased extracellular polysaccharide production characteristic of some biofilm-associated organisms...
April 4, 2018: Journal of Innate Immunity
https://www.readbyqxmd.com/read/29615497/novel-broadly-reactive-anticapsular-antibodies-against-carbapenem-resistant-klebsiella-pneumoniae-protect-from-infection
#7
Elizabeth Diago-Navarro, Michael P Motley, Gonzalo Ruiz-Peréz, Winnie Yu, Julianne Austin, Bruna M S Seco, Guozhi Xiao, Aniska Chikhalya, Peter H Seeberger, Bettina C Fries
Carbapenem-resistant (CR) sequence type 258 (ST258) Klebsiella pneumoniae has become an urgent health care threat, causing an increasing number of high-mortality infections. Its resistance to numerous antibiotics and threat to immunocompromised patients necessitate finding new therapies to combat these infections. Previous successes in the laboratory, as well as the conservation of capsular polysaccharide (CPS) among the members of the ST258 clone, suggest that monoclonal antibody (MAb) therapy targeting the outer polysaccharide capsule of K...
April 3, 2018: MBio
https://www.readbyqxmd.com/read/29602878/molecular-basis-for-the-structural-diversity-in-serogroup-o2-antigen-polysaccharides-in-klebsiella-pneumoniae
#8
Bradley R Clarke, Olga G Ovchinnikova, Steven D Kelly, Monica L Williamson, Jennifer E Butler, Bin Liu, Lu Wang, Xi Gou, Rainer Follador, Todd L Lowary, Chris Whitfield
Klebsiella pneumoniae is a major health threat. Vaccination and passive immunization are considered as alternative therapeutic strategies for managing Klebsiella infections. Lipopolysaccharide O antigens are attractive candidates because of the relatively small range of known O-antigen polysaccharide structures, but immunotherapeutic applications require a complete understanding of the structures found in clinical settings. Currently, the precise number of Klebsiella O antigens is unknown because available serological tests have limited resolution, and their association with defined chemical structures is sometimes uncertain...
March 30, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29547971/potential-targets-for-next-generation-anti-microbial-glycoconjugate-vaccines
#9
Francesca Micoli, Paolo Costantino, Roberto Adamo
Cell surface carbohydrates have been proven optimal targets for vaccine development. Conjugation of polysaccharides to a carrier protein triggers a T-cell dependent immune response to the glycan moiety. Licensed glycoconjugate vaccines are produced by chemical conjugation of capsular polysaccharides to prevent meningitis caused by meningococcus, pneumococcus and Haemophilus influenzae type b. However, other classes of carbohydrates (O-antigens, exopolysaccharides, wall/teichoic acids) represent attractive targets for developing vaccines...
March 14, 2018: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/29535199/antibody-mediated-killing-of-carbapenem-resistant-st258-klebsiella-pneumoniae-by-human-neutrophils
#10
Scott D Kobayashi, Adeline R Porter, Brett Freedman, Ruchi Pandey, Liang Chen, Barry N Kreiswirth, Frank R DeLeo
Carbapenem-resistant Klebsiella pneumoniae is a problem worldwide. A carbapenem-resistant K. pneumoniae lineage classified as multilocus sequence type 258 (ST258) is prominent in the health care setting in many regions of the world, including the United States. ST258 strains can be resistant to virtually all clinically useful antibiotics; treatment of infections caused by these organisms is difficult, and mortality is high. As a step toward promoting development of new therapeutics for ST258 infections, we tested the ability of rabbit antibodies specific for ST258 capsule polysaccharide to enhance human serum bactericidal activity and promote phagocytosis and killing of these bacteria by human neutrophils...
March 13, 2018: MBio
https://www.readbyqxmd.com/read/29530808/biogenic-synthesis-of-copper-nanoparticles-by-natural-polysaccharides-and-pleurotus-ostreatus-fermented-fenugreek-using-gamma-rays-with-antioxidant-and-antimicrobial-potential-towards-some-wound-pathogens
#11
Ahmed I El-Batal, Nawal E Al-Hazmi, Farag M Mosallam, Gharieb S El-Sayyad
Mono-dispersed copper nanoparticles (CuNPs) were constructed using cheap polysaccharides (citrus pectin, chitosan, and sodium alginate), and by appropriating aqueous fermented fenugreek powder (FFP) under the action of Pleurotus ostreatus (as reducing and preserving means), through the influence of gamma irradiation. The synthesized CuNPs are described by UV-Vis. spectroscopy TEM, DLS, XRD, and FT-IR. XRD study of the CuNPs confirmed the generation of metallic CuNPs. The nucleation and the production mechanism of CuNPs are moreover explained...
March 9, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29515763/anticancer-activity-of-biogenerated-silver-nanoparticles-an-integrated-proteomic-investigation
#12
Miriam Buttacavoli, Nadia Ninfa Albanese, Gianluca Di Cara, Rosa Alduina, Claudia Faleri, Michele Gallo, Giuseppe Pizzolanti, Giuseppe Gallo, Salvatore Feo, Franco Baldi, Patrizia Cancemi
Silver nanoparticles (AgNPs), embedded into a specific polysaccharide (EPS), were biogenerated by Klebsiella oxytoca DSM 29614 under aerobic (AgNPs-EPSaer ) and anaerobic conditions (AgNPs-EPSanaer ). Both AgNPs-EPS matrices were tested by MTT assay for cytotoxic activity against human breast (SKBR3 and 8701-BC) and colon (HT-29, HCT 116 and Caco-2) cancer cell lines, revealing AgNPs-EPSaer as the most active, in terms of IC50, with a more pronounced efficacy against breast cancer cell lines. Therefore, colony forming capability, morphological changes, generation of reactive oxygen species (ROS), induction of apoptosis and autophagy, inhibition of migratory and invasive capabilities and proteomic changes were investigated using SKBR3 breast cancer cells with the aim to elucidate AgNPs-EPSaer mode of action...
February 9, 2018: Oncotarget
https://www.readbyqxmd.com/read/29381692/rhinoscleroma-pathogenesis-the-type-k3-capsule-of-klebsiella-rhinoscleromatis-is-a-virulence-factor-not-involved-in-mikulicz-cells-formation
#13
Barbara Corelli, Ana S Almeida, Fabiane Sonego, Virginia Castiglia, Cindy Fevre, Sylvain Brisse, Philippe J Sansonetti, Régis Tournebize
Rhinoscleroma is a human specific chronic granulomatous infection of the nose and upper airways caused by the Gram-negative bacterium Klebsiella pneumoniae subsp. rhinoscleromatis. Although considered a rare disease, it is endemic in low-income countries where hygienic conditions are poor. A hallmark of this pathology is the appearance of atypical foamy monocytes called Mikulicz cells. However, the pathogenesis of rhinoscleroma remains poorly investigated. Capsule polysaccharide (CPS) is a prominent virulence factor in bacteria...
January 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29358888/characterization-of-biofilms-in-biliary-stents-and-potential-factors-involved-in-occlusion
#14
Chetana Vaishnavi, Jayanta Samanta, Rakesh Kochhar
AIM: To quantify the components in biofilms and analyze the predisposing factors involved in occlusion of biliary stents. METHODS: In a prospective study conducted from April 2011 to March 2014 at a tertiary care hospital, all consecutive patients who required endoscopic biliary stent exchange/removal were included. Etiology of the biliary disease was diagnosed by imaging, cytology and on follow-up. Clinical details of patients with biliary stent retrieval were noted...
January 7, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29305504/an-igaa-umob-family-protein-from-serratia-marcescens-regulates-motility-capsular-polysaccharide-biosynthesis-and-secondary-metabolite-production
#15
Nicholas A Stella, Kimberly M Brothers, Jake D Callaghan, Angelina M Passerini, Cihad Sigindere, Preston J Hill, Xinyu Liu, Daniel J Wozniak, Robert M Q Shanks
Secondary metabolites are an important source of pharmaceuticals and key modulators of microbe-microbe interactions. The bacterium Serratia marcescens is part of the Enterobacteriaceae family of eubacteria and produces a number of biologically active secondary metabolites. In this study, we screened for novel regulators of secondary metabolites synthesized by a clinical isolate of S. marcescens and found mutations in a gene for an uncharacterized UmoB/IgaA family member here named gumB Mutation of gumB conferred a severe loss of the secondary metabolites prodigiosin and serratamolide...
March 15, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29297821/isolation-identification-and-genome-analysis-of-lung-pathogenic-klebsiella-pneumoniae-lpkp-in-forest-musk-deer
#16
Wei Zhao, Qing Tian, Yan Luo, Yin Wang, Ze-Xiao Yang, Xue-Ping Yao, Jian-Guo Cheng, Xin Zhou, Wu-You Wang
Klebsiella pneumoniae is an important pathogen commonly associated with opportunistic infections. In this study, lung pathogenic K. pneumoniae (LPKP) was isolated and identified from suppurative pneumoniae in forest musk deer by conventional methods and by 16S ribosomal RNA sequence analysis. Median lethal dose and histopathologic analysis were used to demonstrate pathogenicity of the organism in mice. Furthermore, a draft genome of LPKP was sequenced, and its virulence genes were detected. One hundred and twenty-two virulence genes encoded determinant of capsule polysaccharide (CPS), lipopolysaccharide, fimbriae, outer membrane proteins, iron acquisition, and urease...
December 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/29273737/hydrolytic-activity-determination-of-tail-tubular-protein-a-of-klebsiella-pneumoniae-bacteriophages-towards-saccharide-substrates
#17
Ewa Brzozowska, Anna Pyra, Krzysztof Pawlik, Monika Janik, Sabina Górska, Natalia Urbańska, Zuzanna Drulis-Kawa, Andrzej Gamian
In this paper, the enzymatic activity, substrate specificity and antibiofilm feature of bacteriophage dual-function tail proteins are presented. So far, tail tubular proteins A-TTPAgp31 and TTPAgp44-have been considered as structural proteins of Klebsiella pneumoniae bacteriophages KP32 and KP34, respectively. Our results show that TTPAgp31 is able to hydrolyze maltose as well as Red-starch. The activity of 1 µM of the protein was calculated as 47.6 milli-Units/assay relating to the α-amylase activity. It degrades capsular polysaccharides (cPS), slime polysaccharides (sPS) and lipopolysaccharide (LPS) of K...
December 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29209298/the-gut-entomotype-of-red-palm-weevil-rhynchophorus-ferrugineus-olivier-coleoptera-dryophthoridae-and-their-effect-on-host-nutrition-metabolism
#18
Abrar Muhammad, Ya Fang, Youming Hou, Zhanghong Shi
For invasive insects, the potential roles of gut microbiota in exploiting new food resources and spreading remain elusive. Red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, is an invasive destructive pest which feeds on nutrient-poor tender tissues and has caused extensive mortality of palm trees. The microbes associated with insects can improve their nutrition assimilation. However, experimental evidence on the interactions between RPW and its gut microbiota is still absent. The aim of this study is to determine the dynamics changes and the bacterial entomotype in the RPW gut and its potential physiological roles...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29186063/structural-masquerade-of-plesiomonas-shigelloides-strain-cnctc-78-89-o-antigen-high-resolution-magic-angle-spinning-nmr-reveals-the-modified-d-galactan-i-of-klebsiella-pneumoniae
#19
Karolina Ucieklak, Sabina Koj, Damian Pawelczyk, Tomasz Niedziela
The high-resolution magic angle spinning nuclear magnetic resonance spectroscopy (HR-MAS NMR) analysis of Plesiomonas shigelloides 78/89 lipopolysaccharide directly on bacteria revealed the characteristic structural features of the O -acetylated polysaccharide in the NMR spectra. The O -antigen profiles were unique, yet the pattern of signals in the, spectra along with their ¹H,13 C chemical shift values, resembled these of d-galactan I of Klebsiella pneumoniae . The isolated O- specific polysaccharide (O-PS) of P...
November 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29153063/-investigation-of-various-virulence-factors-of-klebsiella-pneumoniae-strains-isolated-from-nosocomial-infections
#20
Halit Kuş, Uğur Arslan, Hatice Türk Dağı, Duygu Fındık
Klebsiella pneumoniae is an opportunistic pathogen that commonly affects immunosuppressed patients and causes nosocomial infections. K.pneumoniae has a variety of virulence factors, especially capsule polysaccharide, hypermucoviscosity (HV), fimbriae, toxins and determinants for iron acquisition. The aim of this study was to detect the virulence factors in K.pneumoniae strains isolated from nosocomial infections in two years. Fifty three K.pneumoniae strains isolated from the samples of patients with nosocomial infections in the Medical Microbiology Laboratory of Selcuk University Faculty of Medicine Hospital between 2011 and 2013 were included in the study...
October 2017: Mikrobiyoloji Bülteni
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