Bao Nie, Xueqing Chen, Zhuangwei Hou, Miaoxian Guo, Cheng Li, Wenkai Sun, Jiaojiao Ji, Lanlan Zang, Song Yang, Pengxiang Fan, Wenhao Zhang, Hang Li, Yuzhu Tan, Wei Li, Li Wang
Butylphthalide is one of the first-line drugs for ischemic stroke therapy, while no biosynthetic enzyme for butylphthalide has been reported. Here, we present a haplotype-resolved genome of Ligusticum chuanxiong , a long-cultivated and phthalide-rich medicinal plant in Apiaceae. On the basis of comprehensive screening, four Fe(II)- and 2-oxoglutarate-dependent dioxygenases and two CYPs were mined and further biochemically verified as phthalide C-4/C-5 desaturases (P4,5Ds) that effectively promoted the forming of ( S )-3- n -butylphthalide and butylidenephthalide...
February 9, 2024: Science Advances