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https://www.readbyqxmd.com/read/28546550/human-igg2-and-igg4-expressing-memory-b-cells-display-enhanced-molecular-and-phenotypic-signs-of-maturity-and-accumulate-with-age
#1
Britt G de Jong, Hanna IJspeert, Lemelinda Marques, Mirjam van der Burg, Jacques J M van Dongen, Bruno G Loos, Menno C van Zelm
The mechanisms involved in sequential immunoglobulin (IG)G class switching are still largely unknown. Sequential IG class switching is linked to higher levels of somatic hypermutation (SHM) in vivo, but it remains unclear if these are generated temporally during an immune response or upon activation in a secondary response. We here aimed to uncouple these processes and to distinguish memory B cells from primary and secondary immune responses. SHM levels and IgG subclasses were studied with 454 pyrosequencing on PBMC from young children and adults as models for primary and secondary immunological memory...
May 26, 2017: Immunology and Cell Biology
https://www.readbyqxmd.com/read/28544397/efficacy-and-acceptance-of-a-high-velocity-microdroplet-device-for-interdental-cleaning-in-gingivitis-patients-a-monitored-randomized-controlled-trial
#2
I Stauff, Shm Derman, A G Barbe, K C Hoefer, M Bizhang, S Zimmer, M J Noack
OBJECTIVES: To compare in a randomized clinical trial the efficacy of a high-velocity microdroplet device for interdental cleaning vs dental floss at reducing plaque and gingivitis. METHODS: Sixty participants with an irregular interdental home cleaning regime were randomly assigned to use either a microdroplet device (n=40, test) or dental floss (n=20, control) for 4 weeks. At baseline and reassessment, the papilla bleeding index, the modified proximal plaque index and the amount of gingival crevicular fluid (GCF) were recorded...
May 24, 2017: International Journal of Dental Hygiene
https://www.readbyqxmd.com/read/28541550/integrity-of-immunoglobulin-variable-region-is-supported-by-ganp-during-aid-induced-somatic-hypermutation-in-germinal-center-b-cells
#3
Mohammed Mansour Abbas Eid, Mayuko Shimoda, Shailendra Kumar Singh, Sarah Ameen Almofty, Phuong Pham, Myron F Goodman, Kazuhiko Maeda, Nobuo Sakaguchi
Immunoglobulin (Ig) affinity maturation depends on somatic hypermutation (SHM) in variable (V) regions initiated by activation-induced cytidine deaminase (AID). AID induces transition mutations by C→U deamination on both strands, causing C:G→T:A. Error-prone repairs of U by base excision and mismatch repairs create transversion mutations at C/G and mutations at A/T sites. In Neuberger's model, it remained to clarify how transition/transversion repair is regulated. We investigate role of AID-interacting GANP (germinal-center associated nuclear protein) in IgV SHM profile...
May 24, 2017: International Immunology
https://www.readbyqxmd.com/read/28539926/gene-specific-substitution-profiles-describe-the-types-and-frequencies-of-amino-acid-changes-during-antibody-somatic-hypermutation
#4
Zizhang Sheng, Chaim A Schramm, Rui Kong, James C Mullikin, John R Mascola, Peter D Kwong, Lawrence Shapiro
Somatic hypermutation (SHM) plays a critical role in the maturation of antibodies, optimizing recognition initiated by recombination of V(D)J genes. Previous studies have shown that the propensity to mutate is modulated by the context of surrounding nucleotides and that SHM machinery generates biased substitutions. To investigate the intrinsic mutation frequency and substitution bias of SHMs at the amino acid level, we analyzed functional human antibody repertoires and developed mGSSP (method for gene-specific substitution profile), a method to construct amino acid substitution profiles from next-generation sequencing-determined B cell transcripts...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28536306/chronic-lymphocytic-leukemia-with-mutated-ighv4-34-receptors-shared-and-distinct-immunogenetic-features-and-clinical-outcomes
#5
Aliki Xochelli, Panagiotis Baliakas, Ioannis Kavakiotis, Andreas Agathangelidis, Lesley-Ann Sutton, Eva Minga, Stavroula Ntoufa, Eugen Tausch, Xiao-Jie Yan, Tait D Shanafelt, Karla Plevova, Myriam Boudjogra, Davide Rossi, Zadie Davis, Alba Navarro, Yorick Sandberg, Fie Juhl Vojdeman, Lydia Scarfò, Niki Stavroyianni, Andrey Sudarikov, Silvio Veronese, Tatiana Tzenou, Teodora Karan Djurasevic, Mark A Catherwood, Dirk Kienle, Maria Chatzouli, Monica Facco, Jasmin Bahlo, Christiane Pott, Lone Bredo Pedersen, Larry Mansouri, Karin E Smedby, Charles C Chu, Véronique Giudicelli, Marie-Paule Lefranc, Panagiotis Panagiotidis, Gunnar Juliusson, Achilles Anagnostopoulos, Ioannis Vlahavas, Darko Antic, Livio Trentin, Marco Montillo, Carsten U Niemann, Hartmut Dohner, Anton W Langerak, Sarka Pospisilova, Michael Hallek, Elias Campo, Nicholas- Chiorazzi, Nikos Maglaveras, David Oscier, Gianluca Gaidano, Diane F Jelinek, Stephan Stilgenbauer, Ioanna Chouvarda, Nikos Darzentas, Chrysoula Belessi, Frédéric Davi, Anastasia Hadzidimitriou, Richard Rosenquist, Paolo Ghia, Kostas Stamatopoulos
We sought to investigate if B cell receptor immunoglobulin (BcR IG) stereotypy is associated with particular clinicobiological features amongst chronic lymphocytic leukemia (CLL) patients expressing mutated BcR IG (M-CLL) encoded by the IGHV4-34 gene, and also ascertain whether these associations could refine prognostication.<br /> <p>Experimental Design: In a series of 19,907 CLL cases with available immunogenetic information, we identified 339 IGHV4-34 expressing cases assigned to one of the four largest stereotyped M-CLL subsets, namely subsets #4, #16, #29 and #201, and investigated in detail their clinicobiological characteristics and disease outcomes...
May 23, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28531144/impact-damage-localisation-with-piezoelectric-sensors-under-operational-and-environmental-conditions
#6
Mohammad Saleh Salmanpour, Zahra Sharif Khodaei, M H Ferri Aliabadi
Guided-wave structural health monitoring (SHM) systems with piezoelectric sensors are investigated for localisation of barely visible impact damage in CFRP plates under vibration and different thermal conditions. A single baseline set is used in a delay-and-sum algorithm with temperature correction for damage localisation in a large temperature range. Damage localisation is also demonstrated under transient thermal conditions, with signals recorded while the temperature is rapidly decreased. Damage severity due to successive impact events is studied under constant temperature...
May 22, 2017: Sensors
https://www.readbyqxmd.com/read/28515723/different-somatic-hypermutation-levels-among-antibody-subclasses-disclosed-by-a-new-next-generation-sequencing-based-antibody-repertoire-analysis
#7
Kazutaka Kitaura, Hiroshi Yamashita, Hitomi Ayabe, Tadasu Shini, Takaji Matsutani, Ryuji Suzuki
A diverse antibody repertoire is primarily generated by the rearrangement of V, D, and J genes and subsequent somatic hypermutation (SHM). Class-switch recombination (CSR) produces various isotypes and subclasses with different functional properties. Although antibody isotypes and subclasses are considered to be produced by both direct and sequential CSR, it is still not fully understood how SHMs accumulate during the process in which antibody subclasses are generated. Here, we developed a new next-generation sequencing (NGS)-based antibody repertoire analysis capable of identifying all antibody isotype and subclass genes and used it to examine the peripheral blood mononuclear cells of 12 healthy individuals...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28482199/adar-deaminase-a-to-i-editing-of-dna-and-rna-moieties-of-rna-dna-hybrids-has-implications-for-the-mechanism-of-ig-somatic-hypermutation
#8
Edward J Steele, Robyn A Lindley
The implications are discussed of recently published biochemical studies on ADAR-mediated A-to-I DNA and RNA deamination at RNA:DNA hybrids. The significance of these data are related to previous work on strand-biased and codon-context mutation signatures in B lymphocytes and cancer genomes. Those studies have established that there are two significant strand biases at A:T and G:C base pairs, A-site mutations exceed T-site mutations (A>T) by 2.9 fold and G-site mutations exceed C-site mutations (G>C) by 1...
April 21, 2017: DNA Repair
https://www.readbyqxmd.com/read/28471418/multiple-two-way-time-message-exchange-ttme-time-synchronization-for-bridge-monitoring-wireless-sensor-networks
#9
Fanrong Shi, Xianguo Tuo, Simon X Yang, Huailiang Li, Rui Shi
Wireless sensor networks (WSNs) have been widely used to collect valuable information in Structural Health Monitoring (SHM) of bridges, using various sensors, such as temperature, vibration and strain sensors. Since multiple sensors are distributed on the bridge, accurate time synchronization is very important for multi-sensor data fusion and information processing. Based on shape of the bridge, a spanning tree is employed to build linear topology WSNs and achieve time synchronization in this paper. Two-way time message exchange (TTME) and maximum likelihood estimation (MLE) are employed for clock offset estimation...
May 4, 2017: Sensors
https://www.readbyqxmd.com/read/28463745/frequency-selection-for-coda-wave-interferometry-in-concrete-structures
#10
Patrik Fröjd, Peter Ulriksen
This study contributes to the establishment of frequency recommendations for use in coda wave interferometry structural health monitoring (SHM) systems for concrete structures. To this end, codas with widely different central frequencies were used to detect boreholes with different diameters in a large concrete floor slab, and to track increasing damage in a small concrete beam subjected to bending loads. SHM results were obtained for damage that can be simulated by drilled holes on the scale of a few mm or microcracks due to bending...
April 23, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28457696/numerous-ontogenetic-roads-to-mantle-cell-lymphoma-immunogenetic-and-immunohistochemical-evidence
#11
Evi Pouliou, Aliki Xochelli, George Kanellis, Evangelia Stalika, Lesley-Ann Sutton, Alba Navarro, Andreas Agathangelidis, Kypros Dimosthenous, Achilles Anagnostopoulos, Efstratios Patsouris, Penelope Korkolopoulou, Christer Sundstrom, Paolo Ghia, Maurilio Ponzoni, Birgitta Sander, Elias Campo, Richard Rosenquist, Anastasia Hadzidimitriou, Kostas Stamatopoulos, Theodora Papadaki
To obtain insight into the ontogeny of mantle cell lymphoma (MCL), we assessed 206 patients from a morphological, immunohistochemical, and immunogenetic perspective. Our series included nodal (n = 151), extranodal (n = 28), and primary splenic (n = 27) MCL cases. Skewing of the immunoglobulin heavy variable (IGHV) gene repertoire was noted, with only four IGHV genes accounting for 46% of cases and approximately 70% of cases (107/154) bearing an imprint of somatic hypermutation (SHM) ranging from minimal to pronounced...
April 27, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28431268/assessment-of-mutagenicity-of-water-from-lake-sevan-armenia-with-application-of-tradescantia-clone-02
#12
R E Avalyan, E A Aghajanyan, A Khosrovyan, A L Atoyants, A E Simonyan, R M Aroutiounian
For many decades water resources in Armenia have been affected by anthropogenic activity, therefore, a regular bioindication of genotoxic effects of the water bodies is desirable. The genotoxicity of water samples collected from different parts of Lake Sevan were assessed by means of Trad-SHM (stamen hair mutation) assay using Tradescantia (clone 02). Here we report a significant increase in the frequency of somatic mutations and morphological changes in the Tradescantia inflorescences exposed to the water samples compared to the control...
March 31, 2017: Mutation Research
https://www.readbyqxmd.com/read/28416602/antigen-receptor-galaxy-a-user-friendly-web-based-tool-for-analysis-and-visualization-of-t-and-b-cell-receptor-repertoire-data
#13
Hanna IJspeert, Pauline A van Schouwenburg, David van Zessen, Ingrid Pico-Knijnenburg, Andrew P Stubbs, Mirjam van der Burg
Antigen Receptor Galaxy (ARGalaxy) is a Web-based tool for analyses and visualization of TCR and BCR sequencing data of 13 species. ARGalaxy consists of four parts: the demultiplex tool, the international ImMunoGeneTics information system (IMGT) concatenate tool, the immune repertoire pipeline, and the somatic hypermutation (SHM) and class switch recombination (CSR) pipeline. Together they allow the analysis of all different aspects of the immune repertoire. All pipelines can be run independently or combined, depending on the available data and the question of interest...
May 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28388279/investigation-of-aid-dicer-and-drosha-expressions-in-patients-with-chronic-lymphocytic-leukemia
#14
Metin Yusuf Gelmez, Ender Coskunpinar, Basak Saracoglu, Gunnur Deniz, Melih Aktan
Chronic lymphocytic leukemia (CLL) is the most common leukemia in Western countries. Cytogenetic lesions such as del13q14, del11q22.3, and del17p13 are identified in 50-60% of patients. Activation-induced cytidine deaminase (AID) plays a central role in somatic hyper mutation (SHM) and class switch recombination (CSR) and functions on Ig genes, but also target non-Ig genes, and over-expression of AID can lead to point mutations or translocations in non-Ig genes such as IgH/Myc translocation. Dicer and Drosha, which have a role in activation process of miRNA, also act in a double-strand DNA break (DSB) repair mechanism...
April 7, 2017: Immunological Investigations
https://www.readbyqxmd.com/read/28383410/a-novel-system-for-predicting-liver-histopathology-in-patients-with-chronic-hepatitis-b
#15
An-Na Pan, Wang-Wang Xu, Yun-Lin Luo, Huan-Huan Yu, Yi-Bing Hu, Qing-Feng Sun, Ji-Guang Ding, Yang-He Wu
There is currently a lack of reliable, reproducible, and easily applied methods for assessing changes in liver histology in patients in the gray zone phase of chronic hepatitis B (CHB). Therefore, we aimed to develop a novel predictive scoring system to detect significant liver histological changes in these patients.A total of 388 patients in the gray zone phase of CHB who underwent liver biopsy were divided into a training group and a validation group, and their clinical and routinely available laboratory parameters were analyzed using univariate analysis, Spearman correlation analysis, and logistic modeling...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28371650/applications-of-a-nanocomposite-inspired-in-situ-broadband-ultrasonic-sensor-to-acousto-ultrasonics-based-passive-and-active-structural-health-monitoring
#16
Menglong Liu, Zhihui Zeng, Hao Xu, Yaozhong Liao, Limin Zhou, Zhong Zhang, Zhongqing Su
A novel nanocomposite-inspired in-situ broadband ultrasonic sensor previously developed, with carbon black as the nanofiller and polyvinylidene fluoride as the matrix, was networked for acousto-ultrasonic wave-based passive and active structural health monitoring (SHM). Being lightweight and small, this kind of sensor was proven to be capable of perceiving strain perturbation in virtue of the tunneling effect in the formed nanofiller conductive network when acousto-ultrasonic waves traverse the sensor. Proof-of-concept validation was implemented, to examine the sensor performance in responding to acousto-ultrasonic waves in a broad frequency regime: from acoustic emission (AE) of lower frequencies to guided ultrasonic waves (GUWs) of higher frequencies...
March 14, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28369649/functional-anatomy-of-the-immunoglobulin-heavy-chain-3%C3%AE-super-enhancer-needs-not-only-core-enhancer-elements-but-also-their-unique-dna-context
#17
Sandrine Le Noir, François Boyer, Sandrine Lecardeur, Mylène Brousse, Zeliha Oruc, Jeanne Cook-Moreau, Yves Denizot, Michel Cogné
Cis-regulatory elements feature clustered sites for transcription factors, defining core enhancers and have inter-species homology. The mouse IgH 3΄ regulatory region (3'RR), a major B-cell super-enhancer, consists of four of such core enhancers, scattered throughout more than 25 kb of packaging 'junk DNA', the sequence of which is not conserved but follows a unique palindromic architecture which is conserved in all mammalian species. The 3'RR promotes long-range interactions and potential IgH loops with upstream promoters, controlling class switch recombination (CSR) and somatic hypermutation (SHM)...
March 22, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28358346/a-data-driven-diagnostic-framework-for-wind-turbine-structures-a-holistic-approach
#18
Simona Bogoevska, Minas Spiridonakos, Eleni Chatzi, Elena Dumova-Jovanoska, Rudiger Höffer
The complex dynamics of operational wind turbine (WT) structures challenges the applicability of existing structural health monitoring (SHM) strategies for condition assessment. At the center of Europe's renewable energy strategic planning, WT systems call for implementation of strategies that may describe the WT behavior in its complete operational spectrum. The framework proposed in this paper relies on the symbiotic treatment of acting environmental/operational variables and the monitored vibration response of the structure...
March 30, 2017: Sensors
https://www.readbyqxmd.com/read/28327532/a-combination-of-soybean-and-haematococcus-extract-alleviates-ultraviolet-b-induced-photoaging
#19
Jieun Shin, Jong-Eun Kim, Kum-Ju Pak, Jung Il Kang, Tae-Seok Kim, Sang-Yoon Lee, Ik-Hyun Yeo, Jung Han Yoon Park, Jong Hun Kim, Nam Joo Kang, Ki Won Lee
Soybean-derived isoflavones have been investigated for their preventative effects against UV-induced symptoms of skin damage including wrinkle formation and inflammation. Haematococcus pluvialis is a freshwater species of Chlorophyta that contains high concentrations of the natural carotenoid pigment astaxanthin. Astaxanthin is known to be involved in retinoic acid receptor (RAR) signaling and previously been associated with the inhibition of activator protein (AP)-1 dependent transcription. Based on previous studies, we hypothesized that a combination of soy extract (SE) and Haematococcus extract (HE) may prevent UVB-induced photoaging through specific signaling pathways, as measured by UVB-induced wrinkling on hairless mice skin and expression changes in human dermal fibroblasts (HDFs)...
March 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28325206/improving-glycemic-control-safely-in-non-critical-care-patients-a-collaborative-systems-approach-in-nine-hospitals
#20
Gregory A Maynard, Diana Childers, Janet Holdych, Heather Kendall, Tom Hoag, Karen Harrison
BACKGROUND: Practice variations in insulin management and glycemic adverse events led nine Dignity Health hospitals to initiate a collaborative effort to improve hypoglycemia, uncontrolled hyperglycemia, and glycemic control. METHODS: Non-critical care adult inpatients with ≥4 point-of-care blood glucose (BG) readings in a ≥2-day period were included. Balanced glucometric goals for each hospital were individualized to improve performance by 10%-20% from baseline or achieve top performance derived from Society of Hospital Medicine (SHM) benchmarking studies...
April 2017: Joint Commission Journal on Quality and Patient Safety
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