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https://www.readbyqxmd.com/read/27908240/new-fluorescent-macrolide-derivatives-for-studying-interactions-of-antibiotics-and-their-analogs-with-the-ribosomal-exit-tunnel
#1
A G Tereshchenkov, A V Shishkina, V V Karpenko, V A Chertkov, A L Konevega, P S Kasatsky, A A Bogdanov, N V Sumbatyan
Novel fluorescent derivatives of macrolide antibiotics related to tylosin bearing rhodamine, fluorescein, Alexa Fluor 488, BODIPY FL, and nitrobenzoxadiazole (NBD) residues were synthesized. The formation of complexes of these compounds with 70S E. coli ribosomes was studied by measuring the fluorescence polarization depending on the ribosome amount at constant concentration of the fluorescent substance. With the synthesized fluorescent tylosin derivatives, the dissociation constants for ribosome complexes with several known antibiotics and macrolide analogs previously obtained were determined...
October 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27903852/topical-honey-in-the-management-of-pediatric-peritoneal-dialysis-exit-sites
#2
Thomas A Forbes, Loren Shaw, Catherine Quinlan
International guidelines in peritoneal dialysis (PD) advocate for regular application of topical mupirocin in chronic PD exit-site care. A strong evidence base links this treatment to reduced rates of exit-site infections and peritonitis. However, emerging reports of increasing mupirocin resistance and gram-negative infections are threatening the long-term viability of topical antibiotic ointments as a prophylactic treatment. Medical grade honey has multiple proven antibacterial and wound healing properties...
November 2016: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
https://www.readbyqxmd.com/read/27899416/peritoneal-dialysis-access-revision-in-children-causes-interventions-and-outcomes
#3
Dagmara Borzych-Duzalka, T Fazil Aki, Marta Azocar, Colin White, Elizabeth Harvey, Sevgi Mir, Marta Adragna, Erkin Serdaroglu, Rajiv Sinha, Charlotte Samaille, Juan Jose Vanegas, Jameela Kari, Lorena Barbosa, Arvind Bagga, Monica Galanti, Onder Yavascan, Giovanna Leozappa, Maria Szczepanska, Karel Vondrak, Kei-Chiu Tse, Franz Schaefer, Bradley A Warady
BACKGROUND AND OBJECTIVES: Little published information is available about access failure in children undergoing chronic peritoneal dialysis. Our objectives were to evaluate frequency, risk factors, interventions, and outcome of peritoneal dialysis access revision. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Data were derived from 824 incident and 1629 prevalent patients from 105 pediatric nephrology centers enrolled in the International Pediatric Peritoneal Dialysis Network Registry between 2007 and 2015...
November 29, 2016: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/27894240/sumoylation-regulation-of-retina-development-and-functions
#4
L Zhang, D W-C Li
The structure and developmental mechanisms of vertebrate retina are highly conserved. One of the most distinctive events during retinogenesis is the temporally and spatially generation of seven types of retinal cells from the multipotent retinal progenitor cells. The importance and prevalence of SUMOylation in regulation of this process through modulation of gene expression and protein function diversity have been increasingly appreciated. Here, we review the biological significance of SUMOylation in retina development, examine how SUMOylation balances the proliferation and cell cycle exit of retinal progenitor cells, and finally discuss the molecular mechanisms mediating the specification of different retina neurons and photoreceptors through modulation of various transcription factors...
November 28, 2016: Current Molecular Medicine
https://www.readbyqxmd.com/read/27890096/entrance-and-exit-wounds-of-high-velocity-bullet-an-autopsy-analysis-in-the-event-of-dispersing-the-mass-rally-in-bangkok-thailand-may-2010
#5
Vichan Peonim, Smith Srisont, Jitta Udnoon, Sompong Wongwichai, Arisa Thapon, Wisarn Worasuwannarak
Fatal mass casualties by high velocity bullets (HVBs) are rare events in peaceful countries. This study presents 27 forensic autopsy cases with 32 shots fired by 5.56×45mm. HVB (M-16 rifle bullets) during the dispersing the mass rally in Bangkok Thailand, May 2010. It was found that twenty-three (71.88%) typical entrance HVB wounds had round sizes less than the bullet diameters. Most entrance wounds had microtears but no collar abrasion since a HVB has a small streamlined spitzer tip and full metal jacket...
November 2016: Legal Medicine
https://www.readbyqxmd.com/read/27886246/disorder-and-dephasing-as-control-knobs-for-light-transport-in-optical-fiber-cavity-networks
#6
Silvia Viciani, Stefano Gherardini, Manuela Lima, Marco Bellini, Filippo Caruso
Transport phenomena represent a very interdisciplinary topic with applications in many fields of science, such as physics, chemistry, and biology. In this context, the possibility to design a perfectly controllable experimental setup, where to tune and optimize its dynamics parameters, is a challenging but very relevant task to emulate, for instance, the transmission of energy in light harvesting processes. Here, we experimentally build a scalable and controllable transport emulator based on optical fiber cavity networks where the system noise parameters can be finely tuned while maximizing the transfer efficiency...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27878130/an-unusual-case-of-transorificial-abdomino-thoracic-impalement-injury-in-a-child
#7
Hemanshi Shah, Charu Tiwari, Shalika Jayaswal, Keshav Murthy
Impalement injuries are rare in the paediatric age group. Still rarer are injuries which traverse multiple body cavities. Such injuries require multispecialty management at a tertiary care centre. We describe a case of an accidental impalement injury in a 12-year-old boy after a fall from height. The rod was seen passing through the right lung in the paracardiac region, piercing the diaphragm and segment IV of the liver and then coursing anterior to the inferior vena cava in the midline. It was seen passing through the small bowel at multiple places and then coursing behind the bladder, entering the rectum and anal canal...
October 2016: Bulletin of Emergency and Trauma
https://www.readbyqxmd.com/read/27864727/post-translational-modification-by-acetylation-regulates-the-mitochondrial-carnitine-acylcarnitine-transport-protein
#8
Nicola Giangregorio, Annamaria Tonazzi, Lara Console, Cesare Indiveri
The carnitine/acylcarnitine transporter (CACT; SLC25A20) mediates an antiport reaction allowing entry of acyl moieties in the form of acylcarnitines into the mitochondrial matrix and exit of free carnitine. The transport function of CACT is crucial for the β-oxidation pathway. In this work, it has been found that CACT is partially acetylated in rat liver mitochondria as demonstrated by anti-acetyl-lys antibody immunostaining. Acetylation was reversed by the deacetylase Sirtuin 3 in the presence of NAD(+). After treatment of the mitochondrial extract with the deacetylase, the CACT activity, assayed in proteoliposomes, increased...
November 18, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27863241/the-chromatin-remodeler-isw1-is-a-quality-control-factor-that-surveys-nuclear-mrnp-biogenesis
#9
Anna Babour, Qingtang Shen, Julien Dos-Santos, Struan Murray, Alexandre Gay, Drice Challal, Milo Fasken, Benoît Palancade, Anita Corbett, Domenico Libri, Jane Mellor, Catherine Dargemont
Chromatin dynamics play an essential role in regulating DNA transaction processes, but it is unclear whether transcription-associated chromatin modifications control the mRNA ribonucleoparticles (mRNPs) pipeline from synthesis to nuclear exit. Here, we identify the yeast ISW1 chromatin remodeling complex as an unanticipated mRNP nuclear export surveillance factor that retains export-incompetent transcripts near their transcription site. This tethering activity of ISW1 requires chromatin binding and is independent of nucleosome sliding activity or changes in RNA polymerase II processivity...
November 17, 2016: Cell
https://www.readbyqxmd.com/read/27845622/sam68-promotes-self-renewal-and-glycolytic-metabolism-in-mouse-neural-progenitor-cells-by-modulating-aldh1a3-pre-mrna-3-end-processing
#10
Piergiorgio La Rosa, Pamela Bielli, Claudia Compagnucci, Eleonora Cesari, Elisabetta Volpe, Stefano Farioli Vecchioli, Claudio Sette
The balance between self-renewal and differentiation of neural progenitor cells (NPCs) dictates neurogenesis and proper brain development. We found that the RNA- binding protein Sam68 (Khdrbs1) is strongly expressed in neurogenic areas of the neocortex and supports the self-renewing potential of mouse NPCs. Knockout of Khdrbs1 constricted the pool of proliferating NPCs by accelerating their cell cycle exit and differentiation into post-mitotic neurons. Sam68 function was linked to regulation of Aldh1a3 pre-mRNA 3'-end processing...
November 15, 2016: ELife
https://www.readbyqxmd.com/read/27836974/wzb117-inhibits-glut1-mediated-sugar-transport-by-binding-reversibly-at-the-exofacial-sugar-binding-site
#11
Ogooluwa A Ojelabi, Kenneth P Lloyd, Andrew H Simon, Julie K De Zutter, Anthony Carruthers
WZB117 (2-fluoro-6-(m-hydroxybenzoyloxy) phenyl m-hydroxybenzoate) inhibits passive sugar transport in human erythrocytes and cancer cell lines and, by limiting glycolysis, inhibits tumor growth in mice. This study explores how WZB117 inhibits the erythrocyte sugar transporter GLUT1 and examines the transporter isoform-specificity of inhibition. WZB117 reversibly and competitively inhibits erythrocyte 3-O-methylglucose (3MG) uptake with Ki(app) = 6 microM but is a noncompetitive inhibitor of sugar exit. Cytochalasin B (CB) is a reversible, noncompetitive inhibitor of 3MG uptake with Ki(app) = 0...
November 11, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27833845/drosophila-ataxin-2-gene-encodes-two-differentially-expressed-isoforms-and-its-function-in-larval-fat-body-is-crucial-for-development-of-peripheral-tissues
#12
Murilo Carlos Bizam Vianna, Deise Cristina Poleto, Paula Fernanda Gomes, Valéria Valente, Maria Luisa Paçó-Larson
Different isoforms of ataxin-2 are predicted in Drosophila and may underlie different cellular processes. Here, we validated the isoforms B and C of Drosophila ataxin-2 locus (dAtx2), which we found to be expressed in various tissues and at different levels during development. dAtx2-B mRNA was detected at low amounts during all developmental stages, whereas dAtx2-C mRNA levels increase by eightfold from L3 to pupal-adult stages. Higher amounts of dAtx2-B protein were detected in embryos, while dAtx2-C protein was also expressed in higher levels in pupal-adult stages, indicating post-transcriptional control for isoform B and transcription induction for isoform C, respectively...
November 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/27827794/molecular-insights-into-protein-synthesis-with-proline-residues
#13
Sergey Melnikov, Justine Mailliot, Lukas Rigger, Sandro Neuner, Byung-Sik Shin, Gulnara Yusupova, Thomas E Dever, Ronald Micura, Marat Yusupov
Proline is an amino acid with a unique cyclic structure that facilitates the folding of many proteins, but also impedes the rate of peptide bond formation by the ribosome. As a ribosome substrate, proline reacts markedly slower when compared with other amino acids both as a donor and as an acceptor of the nascent peptide. Furthermore, synthesis of peptides with consecutive proline residues triggers ribosome stalling. Here, we report crystal structures of the eukaryotic ribosome bound to analogs of mono- and diprolyl-tRNAs...
December 2016: EMBO Reports
https://www.readbyqxmd.com/read/27826861/a-substrate-trapping-method-for-identification-of-direct-cdc14-phosphatase-targets
#14
Brendan L Powers, Hana Hall, Harry Charbonneau, Mark C Hall
Mitotic exit requires the inactivation of cyclin-dependent kinase (Cdk) activity and reversal of Cdk-mediated phosphorylation events by protein phosphatases. In Saccharomyces cerevisiae the mitotic exit network (MEN) leads to activation and dispersal of the Cdc14 phosphatase throughout the cell following successful chromosome segregation. MEN-released Cdc14 is required for both full Cdk inactivation and dephosphorylation of Cdk substrates. While Cdc14 originally was thought to act broadly on mitotic Cdk substrates, recent biochemical studies revealed that Cdc14 possesses a strong preference for a subset of Cdk phosphorylation sites...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27825827/molecular-mechanisms-of-host-cell-traversal-by-malaria-sporozoites
#15
REVIEW
Annie S P Yang, Justin A Boddey
Malaria is a pernicious infectious disease caused by apicomplexan parasites of the genus Plasmodium. Each year, malaria afflicts over 200million people, causing considerable morbidity, loss to gross domestic product of endemic countries, and more than 420,000 deaths. A central feature of the virulence of malaria parasites is the ability of sporozoite forms injected by a mosquito to navigate from the inoculation site in the skin through host tissues to infect the liver. The ability for sporozoites to traverse through different host cell types is very important for the successful development of parasites within the mammalian host...
November 5, 2016: International Journal for Parasitology
https://www.readbyqxmd.com/read/27818662/multifunctional-and-redundant-roles-of-borrelia-burgdorferi-outer-surface-proteins-in-tissue-adhesion-colonization-and-complement-evasion
#16
REVIEW
Jennifer A Caine, Jenifer Coburn
Borrelia burgdorferi is the causative agent of Lyme disease in the U.S., with at least 25,000 cases reported to the CDC each year. B. burgdorferi is thought to enter and exit the bloodstream to achieve rapid dissemination to distal tissue sites during infection. Travel through the bloodstream requires evasion of immune surveillance and pathogen clearance in the host, a process at which B. burgdorferi is adept. B. burgdorferi encodes greater than 19 adhesive outer surface proteins many of which have been found to bind to host cells or components of the extracellular matrix...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27813252/the-calcium-binding-protein-alg-2-promotes-er-exit-site-localization-and-polymerization-of-tfg
#17
Takashi Kanadome, Hideki Shibata, Keiko Kuwata, Terunao Takahara, Masatoshi Maki
ALG-2 (apoptosis-linked gene 2, a gene product of PDCD6) is a 22-kDa Ca(2+) -binding protein. Accumulating evidence points to a role for ALG-2 as a Ca(2+) -responsive adaptor protein. On binding to Ca(2+) , ALG-2 undergoes a conformational change that facilitates its interaction with various proteins. It also forms a homodimer and heterodimer with peflin, a paralog of ALG-2. However, the differences in cellular roles for the ALG-2 homodimer and ALG-2/peflin heterodimer are unclear. Here, we found that TFG (Trk-fused gene) interacted with the ALG-2 homodimer...
November 4, 2016: FEBS Journal
https://www.readbyqxmd.com/read/27812866/detecting-markers-of-therapy-induced-senescence-in-cancer-cells
#18
Dorothy N Y Fan, Clemens A Schmitt
Therapy-induced senescence (TIS), a lasting chemotherapy-evoked proliferative arrest of tumor cells, has gained increasing attention by cancer researchers because of its' profound biological implications, and by clinical oncologists due to its potential contribution to the long-term outcome of cancer patients post-treatment. Although both apoptosis and senescence represent therapy-inducible, ultimate cell-cycle exit programs, mediated via DNA damage response signaling, apoptotic cell death as the faster and often quantitatively more prominent tumor response has been in the scientific focus for decades...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27805563/computed-tomography-for-the-management-of%C3%A2-exit-site-and-tunnel-infections-in-peritoneal-dialysis-patients%C3%A2
#19
Keina Nozaki, Yuka Kamijo, Mineo Nakatsuka, Takeshi Azuma, Tohru Nakagawa, Hideyo Miyazaki, Tetsuya Fujimura, Hiroshi Fukuhara, Haruki Kume, Yoshitaka Ishibashi, Yukio Homma
PURPOSE: To evaluate the effectiveness of computed tomography (CT) for detection of exit-site and tunnel infections with a Tenckhoff catheter. MATERIALS AND METHODS: The study enrolled patients with exit-site or tunnel infections who underwent ultrasonography (US), CT scans, and subsequent catheter removal or partial catheter reimplantation from 2010 to 2014. Control cases on peritoneal dialysis who underwent abdominal CT scans for other reasons were randomly selected...
December 2016: Clinical Nephrology
https://www.readbyqxmd.com/read/27803308/golgi-transport-1b-regulates-protein-export-from-endoplasmic-reticulum-in-rice-endosperm-cells
#20
Yihua Wang, Feng Liu, Yulong Ren, Yunlong Wang, Xi Liu, Wuhua Long, Di Wang, Jianping Zhu, Xiaopin Zhu, Ruonan Jing, Mingming Wu, Yuanyuan Hao, Ling Jiang, Chunming Wang, Haiyang Wang, Yiqun Bao, Jianmin Wan
Coat protein complex II (COPII) mediates the first step of anterograde transport of newly synthesized proteins from the endoplasmic reticulum (ER) to other endomembrane compartments in eukaryotes. A group of evolutionarily conserved proteins (Sar1, Sec23, Sec24, Sec13 and Sec31) constitutes the basic COPII coat machinery; however, the details of how the COPII coat assembly is regulated remain unclear. Here, we report a protein transport mutant of rice (Oryza sativa), named glutelin precursor accumulation4 (gpa4), which accumulates 57-kDa glutelin precursors, and forms two types of ER-derived abnormal structures...
November 1, 2016: Plant Cell
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