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https://www.readbyqxmd.com/read/28735397/global-proteomic-profiling-and-identification-of-stress-responsive-proteins-using-two-dimensional-gel-electrophoresis
#1
Pragya Barua, Dipak Gayen, Nilesh Vikram Lande, Subhra Chakraborty, Niranjan Chakraborty
Global proteome profiling is a direct representation of the protein set in an organism, organ, tissues, or an organelle. One of the main objectives of proteomic analysis is the comparison and relative quantitation of proteins under a defined set of conditions. Two-dimensional gel electrophoresis (2-DE) has gained prominence over the last 4 decades for successfully aiding differential proteomics, providing visual confirmation of changes in protein abundance, which otherwise cannot be predicted from genome analysis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735372/a-systematic-review-and-taxonomy-of-published-quality-criteria-related-to-the-evaluation-of-user-facing-ehealth-programs
#2
Amit Baumel, Michael L Birnbaum, Madalina Sucala
The purpose of this review was to identify and classify key criteria concepts related to the evaluation of user-facing eHealth programs. In line with the PRISMA statement methodology, computer searches of relevant databases were conducted for studies published between January 1, 2000 and March 1, 2016 that contained explicit quality criteria related to mHealth and eHealth products. Reference lists of included articles, review articles, and grey literature (e.g., books, websites) were searched for additional sources...
August 2017: Journal of Medical Systems
https://www.readbyqxmd.com/read/28735368/mdma-induced-indifference-to-negative-sounds-is-mediated-by-the-5-ht2a-receptor
#3
K P C Kuypers, R de la Torre, Farre, N Pizarro, L Xicota, J G Ramaekers
BACKGROUND: MDMA has been shown to induce feelings of sociability, a positive emotional bias and enhanced empathy. While previous research has used only visual emotional stimuli, communication entails more than that single dimension and it is known that auditory information is also crucial in this process. In addition, it is, however, unclear what the neurobiological mechanism underlying these MDMA effects on social behaviour is. Previously, studies have shown that MDMA-induced emotional excitability and positive mood are linked to the action on the serotonin (5-HT) 2A receptor...
July 22, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28735221/physicochemical-properties-of-ionic-and-non-ionic-biocompatible-hydrogels-in-water-and-cell-culture-conditions-relation-with-type-of-morphologies-of-bovine-fetal-fibroblasts-in-contact-with-the-surfaces
#4
Rebeca Rivero, Fabrisio Alustiza, Virginia Capella, Cecilia Liaudat, Nancy Rodriguez, Pablo Bosch, Cesar Barbero, Claudia Rivarola
Cationic, anionic and non-ionic hydrogels having acrylamide polymer backbones were synthesized via free radical polymerization with N,N-methylenebisacrylamide (BIS) as crosslinker. The chemical structures of the hydrogels were characterized by Fourier Transform Infrared Spectroscopy (FTIR). Physicochemical properties such as swelling kinetic, maximum swelling capacity, volume phase transition temperature (VPTT) and wettability (static water contact angle) of hydrogels swollen in aqueous and cell culture medium, at room and cell culture temperatures were studied...
July 11, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28735212/externally-controlled-involuntary-cognitions-and-their-relations-with-other-representations-in-consciousness
#5
Donish Cushing, Adam Gazzaley, Ezequiel Morsella
Percepts and action-related urges often enter consciousness insuppressibly. The Reflexive Imagery Task (RIT) was developed to investigate how high-level cognitions (e.g., subvocalizations), too, can enter consciousness in this manner. Limitations of the paradigm include (a) that no data have confirmed subjects' introspections about the involuntary subvocalizations, and (b) that, in everyday life, adaptive responses to involuntary cognitions often depend on the nature of the other contents in consciousness. To address a and b, we developed an RIT in which subjects were presented with visual objects and instructed to not think of the object names...
July 20, 2017: Consciousness and Cognition
https://www.readbyqxmd.com/read/28735177/the-visual-system-encodes-others-direction-of-gaze-in-a-first-person-frame-of-reference
#6
Colin J Palmer, Colin W G Clifford
The primate visual system contains specialised neural mechanisms for encoding the direction of others' gaze. A foundational question that we can ask in this domain concerns the frame of reference in which another person's gaze is represented. Electrophysiological recordings in macaque monkeys suggest that direction of gaze might be encoded relative to the observer (i.e., in a first person reference frame) or relative to the head or body of the stimulus (i.e., in a second person reference frame). Here we use sensory adaptation to gaze direction as a psychophysical tool to probe how this property of the environment is represented in the human visual system...
July 20, 2017: Cognition
https://www.readbyqxmd.com/read/28735174/tetherless-near-infrared-control-of-brain-activity-in-behaving-animals-using-fully-implantable-upconversion-microdevices
#7
Ying Wang, Xudong Lin, Xi Chen, Xian Chen, Zhen Xu, Wenchong Zhang, Qinghai Liao, Xin Duan, Xin Wang, Ming Liu, Feng Wang, Jufang He, Peng Shi
Many nanomaterials can be used as sensors or transducers in biomedical research and they form the essential components of transformative novel biotechnologies. In this study, we present an all-optical method for tetherless remote control of neural activity using fully implantable micro-devices based on upconversion technology. Upconversion nanoparticles (UCNPs) were used as transducers to convert near-infrared (NIR) energy to visible light in order to stimulate neurons expressing different opsin proteins. In our setup, UCNPs were packaged in a glass micro-optrode to form an implantable device with superb long-term biocompatibility...
July 12, 2017: Biomaterials
https://www.readbyqxmd.com/read/28735160/longitudinal-assessment-of-gait-quality-in-children-with-bilateral-cerebral-palsy-following-repeated-lower-limb-intramuscular-botulinum-toxin-a-injections
#8
Felicity A Read, Roslyn N Boyd, Lee A Barber
BACKGROUND: Serial lower limb intramuscular Botulinum toxin-A (BoNT-A) injections are administered to children with bilateral spastic cerebral palsy (BCP) to reduce spasticity, improve walking and functional mobility, and delay the need for orthopaedic surgery. Gait quality is clinically assessed following BoNT-A with 2D video gait assessments (2DVGA) using the Edinburgh Visual Gait Score (EVGS). AIM: To determine the effect of three consecutive treatment cycles of lower limb intramuscular BoNT-A injections on gait quality using the EVGS in children with BCP by retrospectively reviewing repeated 2DVGA measures...
July 20, 2017: Research in Developmental Disabilities
https://www.readbyqxmd.com/read/28735134/intraventricular-meningioma-resection-with-post-operative-ischemia-of-the-lateral-geniculate-nucleus
#9
Saman Sizdahkhani, Stephen T Magill, Michael W McDermott
BACKGROUND: Intraventricular meningiomas (IVMs) comprise 0.5-3% of intracranial meningiomas. They often cause obstructive hydrocephalus and are commonly treated with surgical resection or stereotactic radiosurgery. OBJECTIVE: To describe the surgical approaches and resection techniques needed to approach intraventricular tumors, while highlighting the eloquent anatomy and blood supply surrounding the ventricular system in order to avoid neurological injury. METHODS: Two cases of left atrial IVMs that were complicated by postoperative lateral geniculate nucleus (LGN) ischemia and resultant temporary contralateral quadrantanopia are described...
July 19, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28735103/voluntary-activation-of-the-trapezius-muscle-in-cases-with-neck-shoulder-pain-compared-to-healthy-controls
#10
Katrine Tholstrup Bech, Camilla Marie Larsen, Gisela Sjøgaard, Andreas Holtermann, Janet L Taylor, Karen Søgaard
Subjects reporting neck/shoulder pain have been shown to generate less force during maximal voluntary isometric contractions (MVC) of the shoulder muscles compared to healthy controls. This has been suggested to be caused by a pain-related decrease in voluntary activation (VA) rather than lack of muscle mass. The aim of the present study was to investigate VA of the trapezius muscle during MVCs in subjects with and without neck/shoulder pain by use of the twitch interpolation technique. Ten cases suffering from pain and ten age and gender matched, healthy controls were included in the study...
July 14, 2017: Journal of Electromyography and Kinesiology
https://www.readbyqxmd.com/read/28735100/attention-modulates-specific-motor-cortical-circuits-recruited-by-transcranial-magnetic-stimulation
#11
J L Mirdamadi, L Y Suzuki, S K Meehan
Skilled performance and acquisition is dependent upon afferent input to motor cortex. The present study used short-latency afferent inhibition (SAI) to probe how manipulation of sensory afference by attention affects different circuits projecting to pyramidal tract neurons in motor cortex. SAI was assessed in the first dorsal interosseous muscle while participants performed a low or high attention demanding visual detection task. SAI was evoked by preceding a suprathreshold transcranial magnetic stimulus with electrical stimulation of the median nerve at the wrist...
July 19, 2017: Neuroscience
https://www.readbyqxmd.com/read/28735084/a-category-specific-top-down-attentional-set-can-affect-the-neural-responses-outside-the-current-focus-of-attention
#12
Yunpeng Jiang, Xia Wu, Xiaorong Gao
A top-down set can guide attention to enhance the processing of task-relevant objects. Many studies have found that the top-down set can be tuned to a category level. However, it is unclear whether the category-specific top-down set involving a central search task can exist outside the current area of attentional focus. To directly probe the neural responses inside and outside the current focus of attention, we recorded continuous EEG to measure the contralateral ERP components for central targets and the steady-state visual evoked potential (SSVEP) oscillations associated with a flickering checkerboard placed on the visual periphery...
July 19, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28735041/self-assembled-polypeptide-nanoparticles-for-intracellular-irinotecan-delivery
#13
N N Zashikhina, M V Volokitina, V A Korzhikov-Vlakh, I I Tarasenko, A Lavrentieva, T Scheper, E Rhuel, R V Orlova, T B Tennikova, E G Korzhikova-Vlakh
In this research poly(l-lysine)-b-poly(l-leucine) (PLys-b-PLeu) polymersomes were developed. It was shown that the size of nanoparticles depended on pH of self-assembly process and varied from 180 to 650nm. The biodegradation of PLys-b-PLeu nanoparticles was evaluated using in vitro polypeptide hydrolysis in two model enzymatic systems, as well as in human blood plasma. The experiments of the visualization of cellular uptake of rhodamine 6g-loaded and fluorescein-labeled nanoparticles were carried out, which approved the possibility of their penetration into the cells...
July 19, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28735013/simultaneous-intrinsic-signal-imaging-of-auditory-and-visual-cortex-reveals-profound-effects-of-acute-hearing-loss-on-visual-processing
#14
Manuel Teichert, Jürgen Bolz
It has been suggested that primary sensory cortices do not work in isolation but receive subthreshold inputs originating from other senses. However, repercussions of an acute loss of one sense on multimodal sensory processing remain elusive. Here we investigated the early effects of acute hearing loss on visual processing in adult mice. For this, we developed a method to simultaneously map the primary auditory (A1) and visual cortex (V1) using periodic intrinsic optical imaging. We found that reducing sound evoked A1 responsiveness due to the induction of conductive hearing loss (CHL) led to a concomitant increase of visually driven V1 activity...
July 19, 2017: NeuroImage
https://www.readbyqxmd.com/read/28735011/assessment-and-elimination-of-the-effects-of-head-movement-on-meg-resting-state-measures-of-oscillatory-brain-activity
#15
Eirini Messaritaki, Loes Koelewijn, Diana C Dima, Gemma M Williams, Gavin Perry, Krish D Singh
Magnetoencephalography (MEG) is increasingly being used to study brain function because of its excellent temporal resolution and its direct association with brain activity at the neuronal level. One possible cause of error in the analysis of MEG data comes from the fact that participants, even MEG-experienced ones, move their head in the MEG system. Head movement can cause source localization errors during the analysis of MEG data, which can result in the appearance of source variability that does not reflect brain activity...
July 19, 2017: NeuroImage
https://www.readbyqxmd.com/read/28734991/endoscopic-full-thickness-resection-using-suture-loop-needle-t-tag-tissue-anchors-in-the-porcine-stomach-with-video
#16
Akira Dobashi, Elizabeth Rajan, Mary A Knipschield, Christopher J Gostout
BACKGROUND AND AIMS: Endoscopic full-thickness resection (EFTR) is still challenging and a reliable technique is desirable. The aim of this study was to evaluate the feasibility of controlled EFTR using a pseudopolyp made from suture loop needle-T-tag (SLNT) tissue anchors in ex vivo porcine stomachs. METHODS: Five pig stomachs were used. Two concentric circumferential border mucosal incisions were made to facilitate isolating a target lesion for full-thickness excision and pseudopolyp formation...
July 19, 2017: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/28734961/radix-puerariae-modulates-glutamatergic-synaptic-architecture-and-potentiates-functional-synaptic-plasticity-in-primary-hippocampal-neurons
#17
Mohammad Maqueshudul Haque Bhuiyan, Md Nazmul Haque, Md Mohibbullah, Yung Kyu Kim, Il Soo Moon
ETHNOPHARMACOLOGICAL RELEVANCE: Neurologic disorders are frequently characterized by synaptic pathology, including abnormal density and morphology of dendritic spines, synapse loss, and aberrant synaptic signaling and plasticity. Therefore, to promote and/or protect synapses by the use of natural molecules capable of modulating neurodevelopmental events, such as, spinogenesis and synaptic plasticity, could offer a preventive and curative strategy for nervous disorders associated with synaptic pathology...
July 19, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28734956/fast-carotid-artery-mr-angiography-with-compressed-sensing-based-three-dimensional-time-of-fight-sequence
#18
Bo Li, Hao Li, Li Dong, Guofu Huang
OBJECTIVE: In this study, we sought to investigate the feasibility of fast carotid artery MR angiography (MRA) by combining three-dimensional time-of-flight (3D TOF) with compressed sensing method (CS-3D TOF). MATERIALS AND METHODS: A pseudo-sequential phase encoding order was developed for CS-3D TOF to generate hyper-intense vessel and suppress background tissues in under-sampled 3D k-space. Seven healthy volunteers and one patient with carotid artery stenosis were recruited for this study...
July 19, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28734948/the-effectiveness-of-a-simulation-based-flipped-classroom-in-the-acquisition-of-laparoscopic-suturing-skills-in-medical-students-a-pilot-study
#19
Hsin-Yi Chiu, Yi-No Kang, Wei-Lin Wang, Hung-Chang Huang, Chien-Chih Wu, Wayne Hsu, Yiu-Shun Tong, Po-Li Wei
OBJECTIVE: To evaluate the effectiveness of a simulation-based flipped classroom in gaining the laparoscopic skills in medical students. DESIGN: An intervention trial. SETTING: Taipei Medical University Hospital, an academic teaching hospital. PARTICIPANTS AND METHODS: Fifty-nine medical students participating in a 1-hour laparoscopic skill training session were randomly assigned to a conventional classroom (n = 29) or a flipped classroom approach (n = 30) based on their registered order...
July 19, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/28734946/impaired-rhodopsin-generation-in-the-rat-model-of-diabetic-retinopathy
#20
Volha V Malechka, Gennadiy Moiseyev, Yusuke Takahashi, Younghwa Shin, Jian-Xing Ma
Diabetic retinopathy is a common complication of diabetes mellitus. Diabetic patients experience functional deficits in dark adaptation, contrast sensitivity, and color perception before microvascular pathologies become apparent. Here, we evaluated early changes in neural retinal function and in retinoid metabolism in the eye in diabetes. Streptozotocin-induced diabetic rats showed decreased a-wave and b-wave amplitudes of scotopic and photopic electroretinography responses four months after diabetes induction compared to nondiabetic controls...
July 19, 2017: American Journal of Pathology
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