Sabrina Piechota, Maria Marchante, Alexa Giovannini, Bruna Paulsen, Kathryn S Potts, Graham Rockwell, Caroline Aschenberger, Alexander D Noblett, Alexandra B Figueroa, Marta Sanchez, Ferran Barrachina, Klaus Wiemer, Luis Guzman, Pedro Belchin, Merrick Pierson Smela, Patrick R J Fortuna, Pranam Chatterjee, Nam D Tran, Dawn A Kelk, Marcy Forti, Shelby Marcinyshyn, Trozalla Smith, David H McCulloh, Marta-Julia Fernandez-Gonzalez, Baruch Abittan, Silvia Ortiz, Joshua U Klein, Peter Klatsky, Daniel Ordonez-Perez, Christian C Kramme
STUDY QUESTION: Can in vitro maturation (IVM) and developmental competence of human oocytes be improved by co-culture with ovarian support cells (OSCs) derived from human-induced pluripotent stem cells (hiPSCs)? SUMMARY ANSWER: OSC-IVM significantly improves the rates of metaphase II (MII) formation and euploid Day 5 or 6 blastocyst formation, when compared to a commercially available IVM system. WHAT IS KNOWN ALREADY: IVM has historically shown highly variable performance in maturing oocytes and generating oocytes with strong developmental capacity, while limited studies have shown a positive benefit of primary granulosa cell co-culture for IVM...
October 10, 2023: Human Reproduction