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https://www.readbyqxmd.com/read/28731643/transcriptome-based-identification-of-the-optimal-reference-cho-genes-for-normalisation-of-qpcr-data
#1
Adam J Brown, Suzanne Gibson, Diane Hatton, David C James
Real-time quantitative PCR (qPCR) is the standard method for determination of relative changes in mRNA transcript abundance. Analytical accuracy, precision, and reliability are critically dependent on the selection of internal control reference genes. In this study we have identified optimal reference genes that can be utilized universally for qPCR analysis of CHO cell mRNAs. Initially, transcriptomic datasets were analysed to identify eight endogenous genes that exhibited high expression stability across four distinct CHO cell lines sampled in different culture phases...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28703025/patient-satisfaction-of-pharmacist-provided-care-via-clinical-video-teleconferencing
#2
Jessica Hatton, Rachel Chandra, David Lucius, Elizabeth Ciuchta
PURPOSE: Patient satisfaction with the use of telehealth in disease state management provided by pharmacists has not been fully studied. We hypothesized that patient satisfaction with pharmacist-provided consultations via clinical video teleconferencing (CVT) would not differ from face-to-face delivery. METHODS: Patients were recruited from 2 primary care provider sites from September 2015 to May 2016. Patients completed a survey to evaluate their satisfaction and quality of provider-patient communication with the method in which consultation with a pharmacist was provided...
January 1, 2017: Journal of Pharmacy Practice
https://www.readbyqxmd.com/read/28699755/co2-reactive-ionic-liquid-surfactants-for-the-control-of-colloidal-morphology
#3
Paul Brown, Vishnu Sresht, Huseyin Burak Eral, Andrew Fiore, César de la Fuente-Núñez, Marcus O'Mahony, Gabriel P Mendes, William T Heller, Patrick S Doyle, Daniel Blankschtein, T Alan Hatton
This paper reports on a new class of stimuli-responsive surfactant generated from commercially available amphiphiles such as dodecyltrimethylammmonium bromide (DTAB) by substitution of the halide counterion with counterions such as 2-cyanopyrrolide, 1,2,3-triazolide, and L-proline that complex reversibly with CO2. Through a combination of small-angle neutron scattering (SANS), electrical conductivity measurements, thermal gravimetric analysis, and molecular dynamics simulations, we show how small changes in charge reorganization and counterion shape and size induced by complexation with CO2 allow for fine tunability of surfactant properties...
July 12, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28676434/multifunctional-bioactive-glass-and-glass-ceramic-biomaterials-with-antibacterial-properties-for-repair-and-regeneration-of-bone-tissue
#4
REVIEW
João S Fernandes, Piergiorgio Gentile, Ricardo A Pires, Rui L Reis, Paul V Hatton
Bioactive glasses (BGs) and related glass-ceramic biomaterials have been used in bone tissue repair for over 30years. Previous work in this field was comprehensively reviewed including by their inventor Larry Hench, and the key features and properties of BGs are well understood. More recently, attention has focused on their modification to further enhance the osteogenic behaviour, or further compositional changes that may introduce additional properties, such as antimicrobial activity. Evidence is emerging that BGs and related glass-ceramics may be modified in such a way as to simultaneously introduce more than one desirable property...
July 1, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28666768/strategies-for-improving-early-nutritional-outcomes-in-children-with-oesophageal-atresia-and-congenital-diaphragmatic-hernia
#5
REVIEW
Dominic A Fitzgerald, Andrea Kench, Lucy Hatton, Jonathan Karpelowsky
Post-natal growth in surgical lung conditions, such as congenital diaphragmatic hernia and oesophageal atresia with tracheo-oesophageal fistula, is often sub-optimal in the early years of life when lung growth is occurring. Whilst constitutional, behavioural and mechanical factors may contribute to poor feeding and weight gain, there is a common path of management with greater caloric supplementation that may change growth trajectories and potentially lead to better respiratory, anthropometric and cognitive outcomes...
May 31, 2017: Paediatric Respiratory Reviews
https://www.readbyqxmd.com/read/28648562/a-cellular-automaton-based-on-plasma-membrane-turnover-accurately-recapitulates-cell-mechanics-during-epithelial-scattering
#6
Christian F Schmutz, Trey D Hatton, Michael P Blomfield, Benjamin D Chandler, Trent C Benedick, Marc D H Hansen
Epithelial cells can be triggered to actively detach from epithelial tissues and become solitary, migratory and invasive. This process occurs repeatedly in development, where it is termed epithelial-mesenchymal transition (EMT), and can be recapitulated as epithelial scattering in cell culture models. Detachment of cell-cell junctions involves changes in contractile forces, actin cytoskeletal organization, changes in cell-substrate adhesion properties, surface presentation of cell-cell adhesion molecules, and gene expression...
June 22, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28639518/selective-laser-melting-enabled-electrospinning-introducing-complexity-within-electrospun-membranes
#7
Thomas E Paterson, Selina N Beal, Martin E Santocildes-Romero, Alfred T Sidambe, Paul V Hatton, Ilida Ortega Asencio
Additive manufacturing technologies enable the creation of very precise and well-defined structures that can mimic hierarchical features of natural tissues. In this article, we describe the development of a manufacturing technology platform to produce innovative biodegradable membranes that are enhanced with controlled microenvironments produced via a combination of selective laser melting techniques and conventional electrospinning. This work underpins the manufacture of a new generation of biomaterial devices that have significant potential for use as both basic research tools and components of therapeutic implants...
June 2017: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
https://www.readbyqxmd.com/read/28636589/identifying-specificity-groups-in-the-t-cell-receptor-repertoire
#8
Jacob Glanville, Huang Huang, Allison Nau, Olivia Hatton, Lisa E Wagar, Florian Rubelt, Xuhuai Ji, Arnold Han, Sheri M Krams, Christina Pettus, Nikhil Haas, Cecilia S Lindestam Arlehamn, Alessandro Sette, Scott D Boyd, Thomas J Scriba, Olivia M Martinez, Mark M Davis
T cell receptor (TCR) sequences are very diverse, with many more possible sequence combinations than T cells in any one individual. Here we define the minimal requirements for TCR antigen specificity, through an analysis of TCR sequences using a panel of peptide and major histocompatibility complex (pMHC)-tetramer-sorted cells and structural data. From this analysis we developed an algorithm that we term GLIPH (grouping of lymphocyte interactions by paratope hotspots) to cluster TCRs with a high probability of sharing specificity owing to both conserved motifs and global similarity of complementarity-determining region 3 (CDR3) sequences...
June 21, 2017: Nature
https://www.readbyqxmd.com/read/28625628/which-patients-with-es-sclc-are-most-likely-to-benefit-from-more-aggressive-radiotherapy-a-secondary-analysis-of-the-phase-iii-crest-trial
#9
Ben J Slotman, Corinne Faivre-Finn, Harm van Tinteren, Astrid Keijser, John Praag, Joost Knegjens, Matthew Hatton, Iris van Dam, Annija van der Leest, Bart Reymen, Jos Stigt, Kate Haslett, Devashish Tripathi, Egbert F Smit, Suresh Senan
INTRODUCTION: In ES-SCLC patients with residual intrathoracic disease after first-line chemotherapy, the addition of thoracic radiotherapy reduces the risk of intrathoracic recurrence, and improves 2-year survival. To identify patient subgroups for future trials investigating higher dose (extra)thoracic radiotherapy, we investigated the prognostic importance of number and sites of metastases in patients included in the CREST trial. MATERIALS/ METHODS: Additional data on sites and numbers of metastases were collected from individual records of 260 patients from the top 9 recruiting centers in the randomized CREST trial (53% of 495 study patients), which compared thoracic radiotherapy (TRT) to no TRT in ES-SCLC patients after any response to chemotherapy...
June 2017: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
https://www.readbyqxmd.com/read/28610738/blood-pressure-and-blood-sugar-assessment-by-recent-dental-school-graduates
#10
Michael N Hatton, Heather Orom, Patrick L Anders
OBJECTIVE: Recent dental school graduates' willingness to assess blood pressure and blood sugar in practice is positively influenced by having a workplace policy fostering these assessments. Negative influences toward these assessments include practice culture issues and time management concerns. The aim of this study was to determine whether 2010-2014 graduates of the School of Dental Medicine at Buffalo, NY (UB-SDM) continue to assess blood pressure and capillary blood sugar after graduation...
July 2017: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/28585079/new-mammalian-expression-systems
#11
Jie Zhu, Diane Hatton
There are an increasing number of recombinant antibodies and proteins in preclinical and clinical development for therapeutic applications. Mammalian expression systems are key to enabling the production of these molecules, and Chinese hamster ovary (CHO) cell platforms continue to be central to delivery of the stable cell lines required for large-scale production. Increasing pressure on timelines and efficiency, further innovation of molecular formats and the shift to new production systems are driving developments of these CHO cell line platforms...
June 6, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28520834/effect-of-electrode-montage-and-head-position-on-air-conducted-ocular-vestibular-evoked-myogenic-potential
#12
Kathryn Makowiec, Devin L McCaslin, Gary P Jacobson, Kelsey Hatton, John Lee
Purpose: The purpose of this investigation was to identify the optimal recording parameters for evoking the ocular vestibular evoked myogenic potential (oVEMP) using air-conduction stimuli. Method: Subjects were 17 otologically and neurologically intact adults (age: M = 24.18 years, SD = 1.91 years). The oVEMP responses were elicited using a 500-Hz tone burst air-conduction stimulus presented at an intensity of 95 dB nHL. The setting was a balance function laboratory that was part of a large tertiary care otology clinic...
June 13, 2017: American Journal of Audiology
https://www.readbyqxmd.com/read/28504349/polysome-profiling-of-mab-producing-cho-cell-lines-links-translational-control-of-cell-proliferation-and-recombinant-mrna-loading-onto-ribosomes-with-global-and-recombinant-protein-synthesis
#13
Charlotte L Godfrey, Emma J Mead, Olalekan Daramola, Sarah Dunn, Diane Hatton, Ray Field, Gary Pettman, C Mark Smales
mRNA translation is a key process determining growth, proliferation and duration of a Chinese hamster ovary (CHO) cell culture and influences recombinant protein synthesis rate. During bioprocessing, CHO cells can experience stresses leading to reprogramming of translation and decreased global protein synthesis. Here we apply polysome profiling to determine reprogramming and translational capabilities in host and recombinant monoclonal antibody-producing (mAb) CHO cell lines during batch culture. Recombinant cell lines with the fastest cell specific growth rates were those with the highest global translational efficiency...
May 15, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28490404/causes-of-dacryocystorhinostomy-failure-external-versus-endoscopic-approach
#14
Giant C Lin, Christopher D Brook, Mark P Hatton, Ralph Metson
OBJECTIVES: To compare the causes of failure between external and endoscopic dacryocystorhinostomy (DCR) techniques for the treatment of lacrimal obstruction. STUDY DESIGN: A retrospective cohort study. METHODS: The study population consisted of 53 consecutive patients who underwent revision endoscopic DCR from 2002 to 2013 for lacrimal duct obstruction. Identified causes of previous DCR failure were compared between patients whose initial surgery was performed through an external versus an endoscopic approach...
May 1, 2017: American Journal of Rhinology & Allergy
https://www.readbyqxmd.com/read/28463239/subcortical-brain-structure-and-suicidal-behaviour-in-major-depressive-disorder-a-meta-analysis-from-the-enigma-mdd-working-group
#15
M E Rentería, L Schmaal, D P Hibar, B Couvy-Duchesne, L T Strike, N T Mills, G I de Zubicaray, K L McMahon, S E Medland, N A Gillespie, S N Hatton, J Lagopoulos, D J Veltman, N van der Wee, T G M van Erp, K Wittfeld, H J Grabe, A Block, K Hegenscheid, H Völzke, I M Veer, H Walter, K Schnell, E Schramm, C Normann, D Schoepf, C Konrad, B Zurowski, B R Godlewska, P J Cowen, B W J H Penninx, N Jahanshad, P M Thompson, M J Wright, N G Martin, H Christensen, I B Hickie
The aetiology of suicidal behaviour is complex, and knowledge about its neurobiological mechanisms is limited. Neuroimaging methods provide a noninvasive approach to explore the neural correlates of suicide vulnerability in vivo. The ENIGMA-MDD Working Group is an international collaboration evaluating neuroimaging and clinical data from thousands of individuals collected by research groups from around the world. Here we present analyses in a subset sample (n=3097) for whom suicidality data were available. Prevalence of suicidal symptoms among major depressive disorder (MDD) cases ranged between 29 and 69% across cohorts...
May 2, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/28436201/brain-responses-to-mechanical-rectal-stimuli-in-patients-with-faecal-incontinence-an-fmri-study
#16
Naseem Mirbagheri, Sean Hatton, Kheng-Seong Ng, Jim Lagopoulos, Marc A Gladman
AIM: Continence is dependent on anorectal/brain interactions. Consequently, aberrations of the brain-gut axis may be important in the pathophysiology of faecal incontinence (FI) in certain patients. The aim of this study was to assess the feasibility of recording brain responses to rectal mechanical stimuli in patients with FI using functional Magnetic Resonance Imaging (fMRI). METHOD: A prospective, cohort pilot study was performed to assess brain responses during rectal stimulation in 14 patients (4 male, mean [SD] age 62 [15] years)...
April 24, 2017: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
https://www.readbyqxmd.com/read/28415393/the-relationship-between-particle-morphology-and-rheological-properties-in-injectable-nano-hydroxyapatite-bone-graft-substitutes
#17
Y Ryabenkova, A Pinnock, P A Quadros, R L Goodchild, G Möbus, A Crawford, P V Hatton, C A Miller
Biomaterials composed of hydroxyapatite (HA) are currently used for the treatment of bone defects resulting from trauma or surgery. However, hydroxyapatite supplied in the form of a paste is considered a very convenient medical device compared to the materials where HA powder and liquid need to be mixed immediately prior to the bone treatment during surgery. In this study we have tested a series of hydroxyapatite (HA) pastes with varying microstructure and different rheological behaviour to evaluate their injectability and biocompatibility...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28391653/clinical-case-series-describes-a-contraindication-for-serenocem-granules-%C3%A2-in-mastoid-obliteration-our-experience-in-sixty-four-patients
#18
Laura Harrison, Sonia Kumar, Matthew Bull, Paul V Hatton, Ian Bottrill, Christopher Aldren
Mastoid obliteration has been performed with various materials including muscle, fat, cartilage, musculoperiosteal flaps, chips and pâté(1) . Biocompatible materials used since the 1960s, have a number of potential advantages over autologous tissue. They are sterile, available off the shelf without the need for harvest and have a low risk of implanting cholesteatoma.
April 9, 2017: Clinical Otolaryngology
https://www.readbyqxmd.com/read/28369727/n-terminal-or-signal-peptide-sequence-engineering-prevents-truncation-of-human-monoclonal-antibody-light-chains
#19
S J Gibson, N J Bond, S Milne, A Lewis, A Sheriff, G Pettman, R Pradhan, D R Higazi, D Hatton
Monoclonal antibodies (mAbs) contain short N-terminal signal peptides on each individual polypeptide that comprises the mature antibody, targeting them for export from the cell in which they are produced. The signal peptide is cleaved from each heavy chain (Hc) and light chain (Lc) polypeptide after translocation to the ER and prior to secretion. This process is generally highly efficient, producing a high proportion of correctly cleaved Hc and Lc polypeptides. However, mis-cleavage of the signal peptide can occur, resulting in truncation or elongation at the N-terminus of the Hc or Lc...
September 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28368439/pembrolizumab-associated-hemophagocytic-lymphohistiocytosis
#20
D Shah, R Shrestha, R Ramlal, J Hatton, H Saeed
No abstract text is available yet for this article.
June 1, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
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