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https://www.readbyqxmd.com/read/29331832/investigations-on-a-cryopreservation-protocol-for-long-term-storage-of-psittacine-spermatozoa-using-cockatiel-semen-as-an-example
#1
H Schneider, D Fischer, K Failing, C Ehling, S Meinecke-Tillmann, A Wehrend, M Lierz
The aim of the present study was the establishment of an effective protocol for cryopreservation of psittacine semen. Therefore, pooled semen samples of 30 cockatiels (Nymphicus hollandicus) were diluted with modified Lake diluent (1:4), partitioned into four equal parts. Three portions were mixed with three cryoprotectants (dimethylacetamide, dimethyl sulfoxide, glycerol) in 4%, 8% and 12% final concentration, respectively, whereas the 4rth part served as control. Altogether, 96 incremental diluted semen samples were obtained for investigation...
December 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/29327861/prognostic-factors-in-intrauterine-insemination-cycles
#2
Fernanda Sicchieri, Aline Bomfim Silva, Ana Carolina Japur de Sá Rosa E Silva, Paula Andrea de Albuquerque Sales Navarro, Rui Alberto Ferriani, Rosana Maria Dos Reis
OBJECTIVE: This study aimed to evaluate the clinical pregnancy rate of intrauterine insemination cycles in relation to patient age, cause of infertility, ovulation induction method, number of mature follicles and sperm with progressive motility. METHODS: This retrospective observational study included 237 intrauterine insemination cycles performed from 2011 to 2015 at the Assisted Reproduction Service of the Hospital das Clínicas of the Ribeirão Preto Medical School, University of São Paulo...
January 12, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29325880/cryoprotective-effect-of-glutamine-taurine-and-proline-on-post-thaw-semen-quality-and-dna-integrity-of-donkey-spermatozoa
#3
M Bottrel, D Acha, I Ortiz, M Hidalgo, J Gósalvez, J Camisão, J Dorado
This study was conducted to evaluate the effect of amino acid addition to semen on post-thaw quality of donkey spermatozoa. Eighteen ejaculates were pooled and divided into aliquots which were cryopreserved in Gent A® containing 1% ethylene glycol (Gent-EG) and supplemented with 0 (as control), 20, 40, or 60 mM of glutamine, proline, or taurine. The greatest concentration (60 mM) of glutamine and taurine resulted in greater (P < 0.001) post-thaw sperm motility. Amino acid supplementation did not improve (P > 0...
December 30, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29325341/-morphological-characteristics-and-phenotypic-analysis-of-multiple-morphological-abnormalities-in-sperm-flagella
#4
J X Wang, X Y Yang, H B Cheng, L Y Shen, G G Wang, Y C Shi, H Li, S M Yang
Objective: To analyze the sperm morphological characteristics of multiple morphological abnormalities of the sperm flagella (MMAF), and to analyze their common features and subtypes. Methods: Twenty-eight patients with abnormal morphology of flagella were analyzed by semen analysis. The morphological characteristics were analyzed by scanning electron microscopy and transmission electron microscopy. Histological observation of one case of testicular tissue was performed. Results: Of the 28 patients, only 13 patients (46...
December 26, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29323738/an-epididymis-specific-secretory-protein-clpsl2-critically-regulates-sperm-motility-acrosomal-integrity-and-male-fertility
#5
Xiaosheng Lu, Fei Ding, Zizhen Lian, Lei Chen, Zuowu Cao, Yiqing Guan, Ran Chen, Dongqing Cai, Yanhong Yu
The epididymis performs an important role in the maturation of spermatozoa including their acquisition of progressive motility and fertilizing ability. However, the molecular mechanisms that govern these maturational events are still poorly defined. Here we report that Clpsl2, a novel colipase homology, is exclusively expressed in the caupt epididymis and conserved in mammalian. Clpsl2 was secreted into the lumen and covered the acrosome region and principal piece of spermatozoa tail. And during epididymal transit, the binding rate between Clpsl2 protein and the spermatozoa gradually decreased...
January 11, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29322011/safety-evaluation-of-bon-sant%C3%A3-cleanser%C3%A2-polyherbal-in-male-wistar-rats-further-investigations-on-androgenic-and-toxicological-profile
#6
O Awodele, O E Kale, A O Odewabi, M Ekor, B A Salau, A O Adefule-Ositelu
Background: The global increase in acceptance and use of herbal remedies in recent times is still accompanied with poor knowledge of their potential adverse effects and the toxicological implications of their use are underestimated. Methods: Bon-santé Cleanser® (BSC), a polyherbal containing Anogeissus leiocarpus, Terminalia ivorensis, Massularia acuminate and Macuna pruriens, is an "energizer and hormone booster". We assessed the effect of BSC on reproductive function after administration for 60 days in male Wistar rats...
January 2018: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/29320852/exposures-to-atmospheric-pm10-and-pm10-2-5-affect-male-semen-quality-results-of-marhcs-study
#7
Niya Zhou, Changtan Jiang, Qing Chen, Huan Yang, Xiaogang Wang, Peng Zou, Lei Sun, Jiaojiao Liu, Ling Li, Lianbing Li, Linping Huang, Hongqiang Chen, Lin Ao, Ziyuan Zhou, Jinyi Liu, Zhihong Cui, Jia Cao
Studies have shown that the effects of ambient particulate matter (PM) may be related to particle's size. However, results on the relationships between different PM and reproductive health are controversial. To explore the impacts of various PM fractions on male reproductive health, a total of 796 eligible subjects recruited in 2013 baseline investigation. In addition, there were 656 (82.4%) and 568 (71.3%) subjects participated follow-up surveys in 2014 and 2015, respectively. We used multivariable regression analysis and mixed-effect model to investigate the associations between air pollutants PM10, PM10-2...
January 11, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29318466/combined-or-individual-effects-of-dietary-probiotic-pediococcus-acidilactici-and-nucleotide-on-reproductive-performance-in-goldfish-carassius-auratus
#8
Nooshin Mehdinejad, Mohammad Reza Imanpour, Valiollah Jafari
This work evaluated the effect of dietary supplementation of probiotic, Pediococcus acidilactici and nucleotide (combined or individual) on reproductive performance including semen quality (motility and density) and egg indices (egg diameter, ovum diameter, absolute fecundity, relative fecundity, gonadosomatic index, hepatosomatic index, fertilization rate, and hatching rate) in goldfish (Carassius auratus). Fish (46.9 ± 2.15 g) were acclimatized and divided into eight experimental diets supplemented with P...
January 9, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29316840/protective-effect-of-motiliperm-in-varicocele-induced-oxidative-injury-in-rat-testis-by-activating-phosphorylated-inositol-requiring-kinase-1%C3%AE-p-ire1%C3%AE-and-phosphorylated-c-jun-n-terminal-kinase-p-jnk-pathways
#9
Kiran Kumar Soni, Li Tao Zhang, Bo Ram Choi, Keshab Kumar Karna, Jae Hyung You, Yu Seob Shin, Sung Won Lee, Chul Young Kim, Chen Zhao, Han-Jung Chae, Hye Kyung Kim, Jong Kwan Park
CONTEXT: MOTILIPERM was prepared as a mixture of extracts of three medicinal herbs [roots of Morinda officinalis How (Rubiaceae), outer scales of Allium cepa L. (Liliaceae) and seeds of Cuscuta chinensis Lamark (Convolvulaceae)]. OBJECTIVE: To investigate the role of reactive oxygen species (ROS)-based endoplasmic reticulum (ER) stress in a rat model of varicocele and the therapeutic efficacy of MOTILIPERM in this model. MATERIALS AND METHODS: Sixty male rats were divided into five experimental groups: a normal control group (CTR + vehicle), a control group administered MOTILIPERM 200 mg/kg (CTR + M 200), a varicocele-induced control group (VC + vehicle) and two varicocele-induced groups administered MOTILIPERM 100 (VC + M 100) or 200 (VC + M 200) mg/kg for 4 weeks...
December 2018: Pharmaceutical Biology
https://www.readbyqxmd.com/read/29316640/sub-chronic-toxicity-of-the-hydroethanolic-leaf-extract-of-telfairia-occidentalis-hook-f-cucurbitaceae-in-male-rats
#10
Abidemi J Akindele, Joy A Oladimeji-Salami, Ramon A Oyetola, Daniel D Osiagwu
Background: Due to its nutritional and medicinal values, the leaf of Telfairia occidentalis Hook f. (Cucurbitaceae) is consumed in different parts of Nigeria. Acute and sub-chronic toxicity of the hydroethanolic leaf extract of Telfairiaoccidentalis were investigated in this study. Methods: Sixty-four male rats were randomized into four different groups of 16 animals each and were separately administered 80, 400 and 2000 mg/kg T. occidentalis orally (p.o.) for 60 days. Animals were sacrificed and blood samples were collected for hematological and biochemical analyses...
January 6, 2018: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/29315686/effect-of-metabolic-and-antioxidant-supplementation-on-sperm-parameters-in-oligo-astheno-teratozoospermia-with-and-without-varicocele-a-double-blind-placebo-controlled-study
#11
G M Busetto, A Agarwal, A Virmani, G Antonini, G Ragonesi, F Del Giudice, S Micic, V Gentile, E De Berardinis
Since sperm require high energy levels to perform their specialised function, it is vital that essential nutrients are available for spermatozoa when they develop, capacitate and acquire motility. However, they are vulnerable to a lack of energy and excess amounts of reactive oxygen species, which can impair sperm function, lead to immotility, acrosomal reaction impairment, DNA fragmentation and cell death. This monocentric, randomised, double-blind, placebo-controlled trial investigated the effect of 6 months of supplementation with l-carnitine, acetyl-l-carnitine and other micronutrients on sperm quality in 104 subjects with oligo- and/or astheno- and/or teratozoospermia with or without varicocele...
January 7, 2018: Andrologia
https://www.readbyqxmd.com/read/29313750/serum-retinol-folic-acid-and-copper-are-associated-with-sperm-abnormalities-in-men-with-obesity
#12
Berniza Calderón, Vital Hevia, Belén Vega-Piñero, Antonia Martín-Hidalgo, Helena Mendez-Del Sol, Héctor F Escobar-Morreale, José I Botella-Carretero
BACKGROUND AND OBJECTIVES: Micronutrients may influence the development and differentiation of sperm cells. The aim of this study was to assess the possible association of deficiencies in several vitamins and trace elements with sperm abnormalities in men with obesity. PARTICIPANTS AND METHODS: Thirty male patients with moderate to severe obesity and ten lean controls who gave written informed consent were included. Anthropometric parameters were recorded. Hormonal and lipid profiles were analyzed, as well as serum concentrations of zinc, copper, retinol, α-tocopherol, 25-hydroxyvitamin D, cobalamin, and folic acid...
January 9, 2018: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/29312842/bladder-neck-collagen-injection-in-the-treatment-of-congenital-retrograde-ejaculation-a-case-report
#13
Basri Cakiroglu, Orhun Sinanoglu, Ersan Arda
The present study describes the first successful treatment of a congenital retrograde ejaculation case with a submucosal collagen injection to the bladder neck. A 28-year-old male (height 170 cm, weight 80 kg) attended to the urology outpatient clinic with complaints of scattered urine stream and absence of ejaculation. The laboratory tests, including hormone profile (follicle stimulating hormone, luteinizing hormone, and testosterone) and the routine blood count were within normal ranges, whereas semen analysis demonstrated a total absence of ejaculation...
November 3, 2017: Curēus
https://www.readbyqxmd.com/read/29307732/promescent%C3%A2-has-a-cytotoxic-impact-on-fresh-human-sperm-in-vitro
#14
Melissa Sutton, Caitlin Hunn, Roxanne B Summers-Colquitt, Shu-Hung Chen, Shahryar K Kavoussi, Keikhosrow M Kavoussi, Parviz K Kavoussi
OBJECTIVES: To assess the impact of Promescent®, a commonly used topical medication for premature ejaculation (PE), on human sperm motility, forward progression (FP), viability, and sperm DNA fragmentation (SDF) in vitro. METHODS: Aliquots from specimens for diagnostic semen analyses from patients (n=20) presenting to a couple's fertility center andrology laboratory for fertility testing were included after the full diagnostic semen analyses were performed. Samples which met the World Health Organization (WHO) 5th edition criteria as "normal" had Promescent® applied to an aliquot of the sample and motility, FP, viability, and SDF were compared to an aliquot which was not treated with Promescent®...
January 4, 2018: Urology
https://www.readbyqxmd.com/read/29307525/veratridine-sensitive-na-channels-regulate-human-sperm-fertilization-capacity
#15
L Candenas, F M Pinto, A Cejudo-Román, C González-Ravina, M Fernández-Sánchez, N Pérez-Hernández, J Irazusta, N Subirán
AIMS: The sperm plasma membrane contains specific ion channels and transporters that initiate changes in Ca2+, Na+, K+ and H+ ions in the sperm cytoplasm. Ion channels are key regulators of the sperm membrane potential, cytoplasmic Ca2+ and intracellular pH (pHi), which leads to regulate motility, capacitation, acrosome reaction and other physiological processes crucial for successful fertilization. Expression of epithelial sodium channels (ENaC) and voltage-gated sodium channels (Nav) in human spermatozoa has been reported, but the role of Na+ fluxes sodium channels in the regulation of sperm cell function remains poorly understood...
January 4, 2018: Life Sciences
https://www.readbyqxmd.com/read/29306822/celecoxib-a-cyclooxygenase-2-inhibitor-offers-chemoprevention-against-reproductive-and-neurobehavioural-abnormalities-induced-by-atrazine-in-male-wistar-rats
#16
O E Kale, T O Oyesola, F S Raji
The cyclooxygenase-2/prostanoid pathway (COX-2) serves as a potential therapeutic target in various pathological conditions. Thus, the modulatory effect of celecoxib (CXB), a COX-2 inhibitor, in atrazine-induced toxicity was investigated. Five groups (n = 6 rats per group) of adult male Wistar rats received corn oil (2 ml/kg), atrazine (ATZ, 300 mg/kg) and CXB (5.7 mg/kg) respectively and their combinations via the oral route. Results obtained showed reduced (p < 0.05) sperm motility (25.8%) and counts (27...
December 30, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29305108/evaluation-of-the-p53-and-thioredoxin-reductase-in-sperm-from-asthenozoospermic-males-in-comparison-to-normozoospermic-males
#17
Mohmmad-Nabi Moradi, Jamshid Karimi, Iraj Khodadadi, Iraj Amiri, Manoochehr Karami, Massoud Saidijam, Akram Vatannejad, Heidar Tavilani
Thioredoxin (Trx) system has a defensive role against the harmful effect of oxidative stress in sperm. p53 is an important regulator of apoptosis and normal process of spermatogenesis. Regulation of p53 by redox state of the cell and Thioredoxin system has been reported. The aim of this study was to evaluate the ROS level, Thioredoxin reductase (TrxR) activity and p53 protein levels in sperm of asthenozoospermic and normozoospermic males. Semen samples from 80 donors were divided into asthenozoospermic (n=40) and normozoospermic (n=40) groups using the WHO criteria...
January 2, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29303233/high-percentages-of-embryos-with-21-18-or-13-trisomy-are-related-to-advanced-paternal-age-in-donor-egg-cycles
#18
Javier García-Ferreyra, Roly Hilario, Julio Dueñas
OBJECTIVE: Advanced paternal age is related to poor sperm quality; however, little is known on its effect on aneuploidy embryo rates and, more importantly, on chromosomal abnormalities like trisomy 21, 18 and 13. The objective of this study was to evaluate the effect of advanced paternal age on the trisomy rates of the chromosomes 21, 18 or 13 in embryos obtained from donated oocytes. METHODS: A total of 378 embryos, obtained from 52 IVF/ICSI cycles with donated oocytes in conjunction with PGD, were allocated according to paternal age in three groups: Group A: ≤39 years (n=115 embryos), Group B: 40-49 years (n=157 embryos) and Group C: ≥50 year (n=106 embryos)...
January 5, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29299890/comparison-of-the-effects-of-clofibrate-and-silafibrate-on-sperm-parameters-quality-and-sex-hormones-in-male-rats
#19
Masoud Delashoub, Mojtaba Ziaee, Arash Khorrami, Seyed Mahdi Banan-Khojasteh
PURPOSE: Fibrates are drugs widely used for the treatment of hyperlipidemic disorders. Previous studies on a novel analogue of clofibrate, called silafibrate, have shown good lipid lowering effects. This study was designed to assess the role of silafibrate as a peroxisome proliferator-activated receptors (PPARs) agonist on sperm health and spermatogenesis in adult male rats. MATERIAL AND METHODS: Seventy male Wistar rats were randomly allocated into 7 groups: Cl-10, Cl-20, and Cl-40 mg/kg/day (clofibrate); Si-10, Si-20, and Si-40 mg/kg/day (silafibrate); and C, control...
January 3, 2018: Urology Journal
https://www.readbyqxmd.com/read/29296005/physcomitrella-mads-box-genes-regulate-water-supply-and-sperm-movement-for-fertilization
#20
Shizuka Koshimizu, Rumiko Kofuji, Yuko Sasaki-Sekimoto, Masahide Kikkawa, Mie Shimojima, Hiroyuki Ohta, Shuji Shigenobu, Yukiko Kabeya, Yuji Hiwatashi, Yosuke Tamada, Takashi Murata, Mitsuyasu Hasebe
MIKC classic (MIKCC)-type MADS-box genes encode transcription factors that function in various developmental processes, including angiosperm floral organ identity. Phylogenetic analyses of the MIKCC-type MADS-box family, including genes from non-flowering plants, suggest that the increased numbers of these genes in flowering plants is related to their functional divergence; however, their precise functions in non-flowering plants and their evolution throughout land plant diversification are unknown. Here, we show that MIKCC-type MADS-box genes in the moss Physcomitrella patens function in two ways to enable fertilization...
January 3, 2018: Nature Plants
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