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https://www.readbyqxmd.com/read/28919176/different-approaches-to-establish-infertile-rooster
#1
Fereshteh Ghadimi, Malak Shakeri, Mahdi Zhandi, Mojtaba Zaghari, Abbas Piryaei, Parham Moslehifar, Alireza Rajabinejad
Several methods have been developed to suppress spermatogenesis in recipient males before spermatogonial stem cells (SSCs) transplantation. The aim of this study was to compare two different methods of depleting endogenous spermatogenesis in recipient ROSS 308 strain adult roosters. Gamma-radiation and alkylating agent busulfan were utilized to infertilize adult roosters (ROSS 308 strain). Two radiation therapy regimes (based on (60)co isotope) were conducted locally to testes using 40Gy (5×8Gy with three-day intervals) and 30Gy (3×10Gy with three-day intervals)...
August 26, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28915635/dysregulation-of-an-x-linked-primate-specific-epididymal-microrna-cluster-in-unexplained-asthenozoospermia
#2
Xingrong Qing, Jian Shi, Tingting Dong, Chunlin Wu, Lian Hu, Honggang Li
Asthenoszoopermia, characterized by reduced sperm motility, is one of the primary forms of male infertility. Whereas most cases were diagnosed into unexplained asthenozoospermia (UA) because the etiology cannot be identified. In animal models, epigenetic dysregulation in epididymis can impair sperm maturation and result in asthenozoospermia. However, researches of epididymal epigenetic regulation on humans are impeded by the difficulty in obtaining epididymal tissues. We previously identified cell-free seminal microRNAs predominately derived from epididymis in human ejaculate...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28915245/effects-of-gnrh-vaccination-in-wild-and-captive-african-elephant-bulls-loxodonta-africana-on-reproductive-organs-and-semen-quality
#3
Imke Lueders, Debbie Young, Liana Maree, Gerhard van der Horst, Ilse Luther, Stephan Botha, Brendan Tindall, Geoffrey Fosgate, André Ganswindt, Henk J Bertschinger
OBJECTIVES: Although the African elephant (Loxodonta africana) is classified as endangered by the International Union for Conservation of Nature (IUCN), in some isolated habitats in southern Africa, contraception is of major interest due to local overpopulation. GnRH vaccination has been promoted as a non-invasive contraceptive measure for population management of overabundant wildlife. We tested the efficacy of this treatment for fertility control in elephant bulls. METHODS: In total, 17 male African elephants that were treated with a GnRH vaccine were examined in two groups...
2017: PloS One
https://www.readbyqxmd.com/read/28912303/gpr62-constitutively-activates-camp-signaling-but-is-dispensable-for-male-fertility-in-mice
#4
Tomoyuki Muroi, Yuri Matsushima, Ryota Kanamori, Hikari Inoue, Wataru Fujii, Keiichiro Yogo
G-protein-coupled receptors (GPCRs) participate in diverse physiological functions and are promising targets for drug discovery. However, there are still over 140 orphan GPCRs whose functions remain to be elucidated. Gpr62 is one of the orphan GPCRs that is expressed in the rat and human brain. In this study, we found that Gpr62 is also expressed in male germ cells in mice, and its expression increases along with sperm differentiation. GPR62 lacks the BBXXB and DRY motifs, which are conserved across many GPCRs, and it was able to induce cAMP signaling in the absence of a ligand...
September 14, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28911932/varicocelectomy-to-upgrade-semen-quality-to-allow-couples-to-use-less-invasive-forms-of-assisted-reproductive-technology
#5
Mary K Samplaski, Kirk C Lo, Ethan D Grober, Armand Zini, Keith A Jarvi
OBJECTIVE: To determine the magnitude of improvement in semen parameters after a varicocelectomy and the fraction that have improvements such that couples needing IVF or IUI are "upgraded" to needing less invasive assisted reproductive technology (ART). DESIGN: Retrospective review of prospectively collected data. SETTING: Academic medical centers. PATIENT(S): Men presenting for a fertility evaluation with a clinical varicocele...
September 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28911211/are-there-intracellular-ca2-oscillations-correlated-with-flagellar-beating-in-human-sperm-a-three-vs-two-dimensional-analysis
#6
G Corkidi, F Montoya, P Hernández-Herrera, W A Ríos-Herrera, M F Müller, C L Treviño, A Darszon
STUDY QUESTION: Are there intracellular Ca2+ ([Ca2+]i) oscillations correlated with flagellar beating in human sperm? SUMMARY ANSWER: The results reveal statistically significant [Ca2+]i oscillations that are correlated with the human sperm flagellar beating frequency, when measured in three-dimensions (3D). WHAT IS KNOWN ALREADY: Fast [Ca2+]i oscillations that are correlated to the beating flagellar frequency of cells swimming in a restricted volume have been detected in hamster sperm...
September 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28911169/obesity-induced-infertility-in-male-mice-is-associated-with-disruption-of-crisp4-expression-and-sperm-fertilization-capacity
#7
Beatriz C Borges, David Garcia-Galiano, Sanseray da Silveira Cruz-Machado, Xingfa Han, Galina B Gavrilina, Thomas L Saunders, Richard J Auchus, Saher S Hammoud, Gary D Smith, Carol F Elias
Approximately 15% of human couples of reproductive age have impaired fertility, and the male component accounts for about half of these cases. The etiology is usually unknown, but high correlation with the increase in obesity rates is documented. In this study, we show that diet-induced and genetically obese mice display copulatory behavior comparable to controls, but the number of females impregnated by obese males is remarkably low. Screening for changes in gene expression in the male reproductive tract showed decreased Crisp4 expression in testis and epididymis of obese mice...
September 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28904906/a-step-by-step-guide-to-office-based-sperm-retrieval-for-obstructive-azoospermia
#8
REVIEW
Robert M Coward, Jesse N Mills
A variety of surgical options exists for sperm retrieval in the setting of obstructive azoospermia (OA). With appropriate preparation, the majority of these techniques can safely be performed in the office with local anesthesia and with or without monitored anesthesia care (MAC). The available techniques include percutaneous options such as percutaneous epididymal sperm aspiration (PESA) and testicular sperm aspiration (TESA), as well as open techniques that include testicular sperm extraction (TESE) and microsurgical epididymal sperm aspiration (MESA)...
August 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/28904502/antispermatogenic-mechanism-of-trona-is-associated-with-lipid-peroxidation-but-not-testosterone-suppression
#9
Ayodeji F Ajayi, Roland E Akhigbe
BACKGROUND: About half of the cases of infertility in couples have been attributed to male factor. Despite the claim in folklore medicine that trona (a sesquicarbonate or hydrated carbonate of sodium) causes fetal loss, its effect on male reproductive function has not been investigated. AIM: This study sought to provide scientific evidence on the effect of trona on sperm characteristics, male reproductive hormones and organs, and lipid peroxidation. MATERIALS AND METHODS: Forty male Wistar rats of comparable weights were used for the study...
April 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28903836/reconsidering-a-lower-level-of-follicle-stimulating-hormone-as-abnormal-in-sub-fertile-males-of-pakistan
#10
Shehla Arif, Ayesha Khan
OBJECTIVE: To assess the association between Follicle-Stimulating Hormone (FSH) and semen parameters in order to evaluate whether the current laboratory reference for abnormal FSH levels should be readjusted. STUDY DESIGN: Observational, cross-sectional study. PLACE AND DURATION OF STUDY: Infertility Clinic of Gynecology Unit 1, Civil Hospital, Karachi, from May 2015 to April 2016. METHODOLOGY: The study included 100 sub-fertile males inducted from the clinic...
August 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/28894123/mutations-in-the-prostate-specific-antigen-psa-klk3-correlate-with-male-infertility
#11
Nishi Gupta, Digumarthi V S Sudhakar, Pravin Kumar Gangwar, Satya Narayan Sankhwar, Nalini J Gupta, Baidyanath Chakraborty, Kumarasamy Thangaraj, Gopal Gupta, Singh Rajender
Prostate specific antigen (PSA/KLK3) is known to be the chief executor of the fragmentation of semenogelins, dissolution of semen coagulum, thereby releasing sperm for active motility. Recent research has found that semenogelins also play significant roles in sperm fertility by affecting hyaluronidase activity, capacitation and motility, thereby making PSA important for sperm fertility beyond simple semen liquefaction. PSA level in semen has been shown to correlate with sperm motility, suggesting that PSA level/activity can affect fertility...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28893310/effect-of-long-term-storage-in-safe-cell-extender-on-boar-sperm-dna-integrity-and-other-key-sperm-parameters
#12
Wiesław Bielas, Wojciech Niżański, Agnieszka Partyka, Anna Rząsa, Ryszard Mordak
BACKGROUND: There is some controversy about the extent of changes in different sperm cell features in stored boar semen, especially regarding the potential role of the DNA fragmentation assay for assessment of sperm fertilizing ability. The aim of this study was to assess the effect of time of storage and the dynamic changes in sperm cell characteristics in normospermic boar semen stored in long-term extender, in order to determine the susceptibility to damage of particular structures of spermatozoa during cooling and storage at 17 °C for 240 h post collection...
September 11, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28891798/-effect-of-the-cellular-and-humoral-immune-response-in-infertile-couples-and-its-relationship-with-infections
#13
Adriana Brufman, Laura Colombo, Esteban Streiger, Rocío Pusillico
OBJECTIVE: The purpose of this study was to determine the prevalence of different microorganisms isolated from the urogenital tract of infertile men and assess whether there are differences in semen parameters according to the presence or absence of genital infections. METHODS: 280 semen samples from infertile men were studied retrospectively. Semen parameters were analyzed according to the World Health Organization criteria (WHO 2010). Microbiological analysis was performed using the modified method of Stamey and Meares proposed by Santoianni et al...
September 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28891229/effect-of-different-mini-volume-colloid-centrifugation-configurations-on-flow-cytometrically-sorted-sperm-recovery-efficiency-and-quality-using-a-computer-assisted-semen-analyzer
#14
L B Ferré, J L Chitwood, C Fresno, H H Ortega, M E Kjelland, P J Ross
Straws of sex-sorted sperm are usually packaged at a low concentration (e.g., ~2.1 × 10(6)  sperm/ml) and cost significantly more than unsorted conventional semen from the same sire. In order to maximize the efficiency of using sex-sorted sperm under in vitro fertilization conditions, the selection of an appropriate sperm separation technique is essential. In this study, the effect of using different silane-coated silica colloid dilutions and layering configurations during centrifugation of sex-sorted sperm was examined over an extended period of incubation time...
September 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28891125/effect-of-cryoprotectants-and-thawing-temperatures-on-chicken-sperm-quality
#15
M Miranda, B Kulíková, J Vašíček, L Olexiková, N Iaffaldano, P Chrenek
There is need for standardization of freezing-thawing protocol for rooster semen to minimize variability among results. Therefore, we aimed to compare effect of four different permeating cryoprotectants and two thawing temperatures (37 vs. 5°C) on sperm post-thaw motility and to analyse combined effect of the best permeating cryoprotectant (P-CPA) with one of four non-permeating cryoprotectants (N-CPA) on post-thaw quality of rooster semen evaluated in vitro. Pooled semen from Ross PM3 rooster heavy line was diluted in Kobidil extender and frozen in cryoprotectant solution containing 6% dimethylacetamide, 7...
September 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28890972/nanomechanical-sensors-for-direct-and-rapid-characterization-of-sperm-motility-based-on-nanoscale-vibrations
#16
Shangquan Wu, Zhiguo Zhang, Xiarong Zhou, Hong Liu, Changguo Xue, Gang Zhao, Yunxia Cao, Qingchuan Zhang, Xiaoping Wu
Infertility, whether of male or female origin, is a critical challenge facing the low birth rate and aging population throughout the world, and semen analysis is a cornerstone of the diagnostic evaluation of the male contribution to infertility. This means that tools which can characterize sperm properties in an effective manner are very much needed. The conventional approaches are essentially image-based, which have a limited value for analyzing sperm properties. Here, we show that an assay using nanomechanical sensors can detect sperm motility based on nanomotion...
September 11, 2017: Nanoscale
https://www.readbyqxmd.com/read/28890522/characterization-of-the-testicular-regeneration-potential-in-premature-cockerels
#17
Po-Liang Cheng, Hui-Ru Wu, Cheng-Yan Li, Chih-Feng Chen, Hsu-Chen Cheng
Previous studies have shown that grafted neonatal chicken testicular tissue can develop and produce functional sperm; however, it was unclear whether regenerative processes or proportional growth caused the re-appearance of spermatogenic tissue. We dissociated testicular tissues, performed subcutaneous auto-transplantation of the re-aggregated cells to castrated cockerels, and monitored the post-surgery development of these transplanted aggregates. We found that these transplanted cell aggregates experienced compensatory growth in the form of a 300-fold increase in size, rather than the 30-fold increase observed in normal testis development...
September 8, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28887675/how-bad-is-aluminum-exposure-to-reproductive-parameters-in-rats
#18
Viviane G S Mouro, Tatiana P Menezes, Graziela D A Lima, Rafael R Domingues, Ana Cláudia F Souza, Juraci A Oliveira, Sérgio L P Matta, Mariana Machado-Neves
Aluminum (Al) is the most widely distributed metal in the environment and is extensively used in human daily life without any known biological function. It is known that exposure to high concentrations of Al impacts negatively on serum testosterone levels, testicular histomorphometry, and sperm parameters; however, no information is available about the effects of low exposure levels on reproduction. International organizations have established the Al concentration tolerated in drinking water as 3.35 × 10(-4) mg/kg...
September 8, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28887327/uterine-flushings-from-women-treated-with-levonorgestrel-affect-sperm-functionality-in-vitro
#19
Mayel Chirinos, Marta Durand, María Elena González-González, Gabriela Hernández-Silva, Israel Maldonado-Rosas, Pablo Lopez, Fernando Larrea
Levonorgestrel (LNG), a synthetic 19 nor-testosterone derivative, is widely used for emergency contraception. It is well known that LNG prevents ovulation only when given prior to the surge of serum luteinizing hormone (LH) during the periovulatory phase of the menstrual cycle. This observation suggests that LNG, given its contraceptive efficacy, has additional effects other than those affecting ovulation. In this study, we have evaluated the effects on human sperm functionality of uterine flushings (UF) obtained from women at day LH+1 of a control cycle (CTR-LH+1) and after receiving LNG (LNG-LH+1) two days before the surge of LH...
September 8, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28886262/effectiveness-of-antibiotic-treatment-in-infertile-patients-with-sterile-leukocytospermia-induced-by-tobacco-use
#20
Bogdan Pajovic, Ljiljana Pajovic, Marko Vukovic
Our study investigated whether antibiotic therapy in infertile tobacco users with sterile leukocytospermia and a history of former sexual transmitted disease (STD) has an advantage over cessation of smoking alone. For this study, 80 male infertile smokers were divided into two subgroups. The first group numbered 40 patients treated with a two week course of doxycycline, 100 mg, twice daily, while the second group simply ceased smoking. A control group of 20 patients with chronic abacterial prostatitis (CPPS III a) were treated with the same antibiotic for two weeks...
September 8, 2017: Systems Biology in Reproductive Medicine
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