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https://www.readbyqxmd.com/read/28314435/effect-of-green-tea-camellia-sinensis-extract-and-pre-freezing-equilibration-time-on-the-post-thawing-quality-of-ram-semen-cryopreserved-in-a-soybean-lecithin-based-extender
#1
Mahdieh Mehdipour, Hossein Daghigh Kia, Abouzar Najafi, Hossein Vaseghi Dodaran, Olga García-Álvarez
The aim of this study was to determine the effect of Camellia sinensis extract as antioxidant supplement and pre-freezing equilibration times in a soybean lecithin extender for freezing ram semen. In this study, a total of 20 ejaculates were collected from four Ghezel rams and diluted with extenders (1.5% soybean lecithin, 7% glycerol) containing no supplements (control) and Camellia sinensis extract (5, 10, and 15 mg/L) and cryopreserved, immediately after thermal equilibrium was reached at 5 °C (0 h), or 4 h after equilibration...
December 2016: Cryobiology
https://www.readbyqxmd.com/read/28303993/predictive-factors-for-pregnancy-outcome-following-controlled-ovarian-stimulation-and-intrauterine-insemination
#2
Goncayetkin Yildirim, Lale Susan Turkgeldi, Nadiye Koroglu
OBJECTIVE: To establish predictive factors for positive pregnancy outcome in cases of controlled ovarian stimulation and intrauterine insemination. METHODS: The retrospective study was conducted at Kanuni Sultan Suleyman Training and Research Hospital and comprised subjects having undergone ovulation induction cycles and intrauterine insemination between June 2010 and June 2015. Data was analysed in terms of various parameters affecting clinical pregnancy rates...
March 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28302333/analysis-of-common-carp-cyprinus-carpio-sperm-motility-and-lipid-composition-using-different-in-vitro-temperatures
#3
Hadiseh Dadras, Sabine Sampels, Amin Golpour, Viktoriya Dzyuba, Jacky Cosson, Borys Dzyuba
In fish, sperm quality is frequently associated with sperm motility variables. The response of sperm motility to different temperatures varies among species and plasma membrane lipid composition may contribute to variations in findings in previous research. In the present study, sperm motility and lipid composition were analysed between motile or immotile carp Cyprinus carpio sperm at different in vitro temperatures (4, 14 and 24°C). The duration of the period over which sperm motility is sustained was longer at 4°C compared with 14 and 24°C; while sperm velocity was greatest at 24°C...
March 6, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28301256/sperm-epigenetics-in-the-study-of-male-fertility-offspring-health-and-potential-clinical-applications
#4
Timothy G Jenkins, Kenneth I Aston, Emma R James, Douglas T Carrell
The mammalian sperm contains a highly unique and specialized epigenetic landscape that offers a great degree of interesting research opportunities. One key discriminating feature of the mature sperm epigenome is that it, in theory, represents both remnant marks used throughout spermatogenesis to generate sperm cells competent to perform their function, but also marks that appear to be useful beyond fertilization. Key questions must be asked about the utility of these marks and the multiple purposes that may be served...
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28301253/efficacy-and-safety-of-varicocelectomies-a-meta-analysis
#5
Renbin Yuan, Hui Zhuo, Dehong Cao, Qiang Wei
This study reviewed the efficacy and safety of the three surgical approaches for varicocele (microsurgical, laparoscopic, and open varicocelectomy). A systematic review of the relevant randomized clinical trials was performed. Trials were identified from specialized trials register of the Cochrane UGDP Group, the Cochrane library, additional electronic searches (mainly MEDLINE, EMBSAE, SCI, CBM), and handsearching. Clinical trials comparing microsurgical, laparoscopic and open varicocelectomies were included...
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28299523/predictors-for-spontaneous-pregnancy-after-microsurgical-subinguinal-varicocelectomy-a-prospective-cohort-study
#6
Jian-Wei Zhang, Quan-Quan Xu, You-Lin Kuang, Yan Wang, Feng Xu, Yu-Dong Tian
PURPOSE: The purpose of this study is to determine the possible preoperative predictors of spontaneous pregnancy (SPR) for infertile males with varicocele after microsurgical subinguinal varicocelectomy (MVL) performed in two medical centers in a prospective cohort study. METHODS: A total of 120 males with varicocele that underwent MVL between June 2013 and June 2014 in two medical centers were documented. Related data, including male and female partner age, male body mass index (BMI), female BMI, preoperative semen parameters, hormone levels, testicular volume, grade and side of varicocele, were collected and analyzed...
March 15, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28298440/structural-basis-of-outer-dynein-arm-intraflagellar-transport-by-the-transport-adaptor-protein-oda16-and-the-intraflagellar-transport-protein-ift46
#7
Michael Taschner, André Mourão, Mayanka Awasthi, Jerome Basquin, Esben Lorentzen
Motile cilia are found on unicellular organisms such as the green alga Chlamydomonas reinhardtii, on sperm cells, and on cells that line the trachea and fallopian tubes in mammals. The motility of cilia relies on a number of large protein complexes including the force-generating outer dynein arms (ODAs). The transport of ODAs into cilia has been previously shown to require the transport adaptor ODA16 as well as the intraflagellar transport (IFT) protein IFT46, but the molecular mechanism by which ODAs are recognized and transported into motile cilia is still unclear...
March 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28298247/tissue-specific-inhibition-of-urokinase-type-plasminogen-activator-expression-in-the-testes-of-mice-by-inducible-lentiviral-rna-interference-causes-male-infertility
#8
Kai Zhao, Yan Liu, Zhe Xiong, Lian Hu, Cheng-Liang Xiong
Urokinase-type plasminogen activator (uPA) is involved in many physiological processes, including male infertility. To explore the effects of uPA in male reproduction, we constructed an inducible uPA short hairpin RNA (shRNA) system expressed by lentiviral vectors. After proving inhibition of uPA expression in the mouse Sertoli cell line TM4 by 1μgmL-1 doxycycline (Dox), two lentivirus (pLenti4-shRNA and pLenti6/TR) were co-microinjected into mouse testes to produce TetR&shuPA mice model. Though oral gavage by 0...
March 16, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28297560/an-investigation-of-the-potential-effect-of-sperm-nuclear-vacuoles-in-human-spermatozoa-on-dna-fragmentation-using-a-neutral-and-alkaline-comet-assay
#9
E Pastuszek, J Kiewisz, P Skowronska, J Liss, M Lukaszuk, A Bruszczynska, G Jakiel, K Lukaszuk
Presence of vacuoles and degree of sperm DNA damage are considered to be the basic factors used for the assessment of sperm fertilization capacity. We aimed to investigate the link between these two parameters. According to our knowledge, this is the first study where the Comet assay was used to assess the degree of DNA fragmentation of sperm categorized by Motile Sperm Organelle Morphology Examination (MSOME) Grades. Semen samples from 10 patients were assessed. Spermatozoa were graded into four MSOME groups according to the Vanderzwalmen's criteria...
March 2017: Andrology
https://www.readbyqxmd.com/read/28296285/elevated-seminal-plasma-myeloperoxidase-is-associated-with-a-decreased-sperm-concentration-in-young-men
#10
J M Pullar, A C Carr, S M Bozonet, P Rosengrave, A J Kettle, M C M Vissers
Myeloperoxidase is a major neutrophil protein which generates oxidants that are highly reactive, and if present in seminal fluid, could be potentially damaging to spermatozoa. We recruited young males aged 18-35 years, unscreened for fertility status, for a pilot study measuring seminal plasma myeloperoxidase. On three occasions, over a 3-month period, we measured parameters of semen quality and correlated these with seminal myeloperoxidase protein and activity. After baseline measurement, participants were supplemented daily with 250 mg of vitamin C, a potent scavenger of reactive oxygen species with antiinflammatory activities...
March 10, 2017: Andrology
https://www.readbyqxmd.com/read/28296254/short-term-fsh-treatment-and-sperm-maturation-a-prospective-study-in-idiopathic-infertile-men
#11
E Casamonti, S Vinci, E Serra, M G Fino, S Brilli, F Lotti, M Maggi, M E Coccia, G Forti, C Krausz
The standard FSH treatment is based on a 3 months period, after which both quantitative/qualitative improvement of sperm parameters and increased pregnancy rate were reported. In this prospective clinical trial, for the first time, we studied (i) Sperm hyaluronic acid binding capacity after highly purified FSH (hpFSH) treatment; (ii) the effect after short-term and standard treatment on this functional parameter. As secondary objective, we analyzed three SNPs on FSHβ and FSHR genes to define their potential predictive value for responsiveness...
March 15, 2017: Andrology
https://www.readbyqxmd.com/read/28295950/sulforaphane-attenuates-di-n-butylphthalate-induced-reproductive-damage-in-pubertal-mice-involvement-of-the-nrf2-antioxidant-system
#12
Xu-Ping Jiang, Jing-Yuan Tang, Zhen Xu, Peng Han, Zhi-Qiang Qin, Cheng-di Yang, Shang-Qian Wang, Min Tang, Wei Wang, Chao Qin, Yang Xu, Bai-Xin Shen, Wei-Min Zhou, Wei Zhang
di-N-butylphthalate (DBP) is a ubiquitous environmental pollutant used for plastic coating and in the cosmetics industry. It has toxic effects on body health, especially the male reproductive system. Here, we investigated the effects of DBP on the male reproductive system of pubertal mice and explored the protective role of sulforaphane (SFN). The results showed that DBP significantly reduced the anogenital distance, testicular weight, sperm count and motility, and plasma and testicular testosterone levels and significantly increased the oxidative stress, sperm abnormalities, and testicular cell apoptosis...
March 15, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28295662/chilled-and-post-thaw-storage-of-sperm-in-different-goldfish-types
#13
G Bernáth, I Ittzés, Z Szabó, Á Horváth, S Krejszeff, J Lujić, L Várkonyi, B Urbányi, Z Bokor
The effective storage time of sperm after stripping (for 48 hr in 6-hr intervals) and after thawing (for 6 hr in 2-hr intervals) in Black moor, Oranda and Calico goldfish types was investigated. Variations in sperm density were also measured in all lines. The efficiency of a sperm cryopreservation method formerly developed for common carp was recorded in all three goldfish lines. Motility parameters ((pMOT, %), curvilinear velocity (VCL, μm/s) and straightness (STR, %)) of Black moor sperm did not decrease significantly during 48 hr of storage...
March 12, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28295487/sperm-dna-fragmentation-as-a-result-of-ultra-endurance-exercise-training-in-male-athletes
#14
D Vaamonde, C Algar-Santacruz, A Abbasi, J M García-Manso
Intensive sports practice seems to exert negative effects on semen parameters; in order to assess these effects, the objective of this study was to assess semen, including DNA fragmentation, and hormone parameters in elite triathletes. Twelve high-level triathletes preparing for a National Triathlon Championship participated in the study. The qualitative sperm parameters analysed were volume, sperm count, motility, morphology and DNA fragmentation; when needed, additional testing was performed. Assessed hormones were testosterone (T), cortisol (C) and testosterone-cortisol ratio (T/C)...
March 15, 2017: Andrologia
https://www.readbyqxmd.com/read/28295462/ferulic-acid-prevents-lead-induced-testicular-oxidative-stress-and-suppressed-spermatogenesis-in-rats
#15
P Hasanein, F Fazeli, M Parviz, M Roghani
Lead affects multiple organ systems including testis. We investigated the effects of ferulic acid (FA) on lead-induced oxidative stress and spermatogenesis suppression in rats. Animals received lead acetate (500 mg/L in drinking water) and/or FA (50 mg/kg, i.g.) for eight weeks. Lead increased testicular malondialdehyde (MDA) and nitrite levels and decreased glutathione (GSH) content and catalase (CAT) activity. Lead decreased testis weight and testosterone level. Sperm parameters decreased in lead group...
March 10, 2017: Andrologia
https://www.readbyqxmd.com/read/28294638/potential-targetability-of-multi-walled-carbon-nanotube-loaded-with-silver-nanoparticles-photosynthesized-from-ocimum-tenuiflorum-tulsi-extract-in-fertility-diagnosis
#16
Pradeep K Jha, Rakhi K Jha, Dilip Rout, Sathishkumar Gnanasekar, Suresh Vs Rana, Maidul Hossain
Nanocarrier mediated targeted delivery and biosensing in reproductive health care is a major exploratory domain. This work demonstrates the loading of silver nanoparticle (AgNP) inside the multiwalled Carbon nanotube(MWCNT) and their targetability to the intracellular part of the sperm cell for its further application in biosensing based infertility diagnosis. Ocimum tenuiflorum (tulsi extract) mediated photosynthesized AgNP exhibited spherical shape, 5-40nm size and surface plasmonic resonance at 430 nm...
March 15, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28294377/cumene-hydroperoxide-induced-changes-in-oxidation-reduction-potential-in-fresh-and-frozen-seminal-ejaculates
#17
A Agarwal, R Sharma, R Henkel, S Roychoudhury, S C Sikka, S du Plessis, Y B Sarda, C Speyer, M Nouh, C Douglas, Z Kayali, E S Ahmed, E Sabanegh
Oxidation-reduction potential (ORP) is a newer integrated measure of the balance between total oxidants (reactive oxygen species-ROS) and reductants (antioxidants) that reflects oxidative stress in a biological system. This study measures ORP and evaluates the effect of exogenous induction of oxidative stress by cumene hydroperoxide (CH) on ORP in fresh and frozen semen using the MiOXSYS Analyzer. Semen samples from healthy donors (n = 20) were collected and evaluated for sperm parameters. All samples were then flash-frozen at -80°C...
March 15, 2017: Andrologia
https://www.readbyqxmd.com/read/28292616/strong-adherence-to-a-healthy-dietary-pattern-is-associated-with-better-semen-quality-especially-in-men-with-poor-semen-quality
#18
Elsje C Oostingh, Régine P M Steegers-Theunissen, Jeanne H M de Vries, Joop S E Laven, Maria P H Koster
OBJECTIVE: To study associations between periconceptional dietary patterns and semen quality parameters. DESIGN: Prospective periconception cohort study. SETTING: Tertiary hospital. PATIENT(S): One hundred and twenty-nine male partners of pregnant women who participated in the Rotterdam Periconception Cohort (Predict study). INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Semen quality parameters-ejaculate volume, sperm concentration, total sperm count, progressive motility, immotile sperm, and total motile sperm count (TMSC)...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28291811/systematic-identification-and-characterization-of-long-non-coding-rnas-in-mouse-mature-sperm
#19
Xiaoning Zhang, Fengxin Gao, Jianbo Fu, Peng Zhang, Yuqing Wang, Xuhui Zeng
Increasing studies have shown that mature spermatozoa contain many transcripts including mRNAs and miRNAs. However, the expression profile of long non-coding RNAs (lncRNAs) in mammalian sperm has not been systematically investigated. Here, we used highly purified RNA to investigate lncRNA expression profiles in mouse mature sperm by stranded-specific RNA-seq. We identified 20,907 known and 4,088 novel lncRNAs transcripts, and the existence of intact lncRNAs was confirmed by RT-PCR and fluorescence in situ hybridization on two representative lncRNAs...
2017: PloS One
https://www.readbyqxmd.com/read/28288491/effect-of-traditional-treatment-of-diabetes-mellitus-with-xanthosoma-sagittifolium-on-the-male-reproductive-system-of-alloxan-induced-diabetic-wistar-rats
#20
Olayinka A Oridupa, Oluyemisi F Folasire, Adedotun J Owolabi, Oluwasanmi Aina
The management of diabetes mellitus (DM) targets glycemic control as well as prevention or reversal of other complications associated with the metabolic disorder. One of such complications is the infertility which has been traced to oxidative stress and DNA damage caused by DM. In this study, experimentally-induced diabetic male Wistar rats were fed with Xanthosoma sagittifolium (cocoyam) for 14 days and its effect on sperm morphology and characteristics, as well as histology of the testes were assessed. X...
March 13, 2017: Drug Research
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