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https://www.readbyqxmd.com/read/28610788/evaluation-of-antigen-specific-interleukin-1%C3%AE-as-a-biomarker-to-detect-cattle-infected-with-mycobacterium-bovis
#1
Mahmoud M Elnaggar, Gaber S Abdellrazeq, Alaa Elsisy, Asmaa H Mahmoud, Abdelrazeq Shyboub, Martina Sester, Samy A Khaliel, Mahavir Singh, Helmy A Torky, William C Davis
Bovine tuberculosis (bTB) is a major world-wide health problem that has been difficult to control, due to the lack of an effective vaccine and limited ability of the tuberculin skin test (TST) and the ancillary whole blood interferon-gamma (IFN-γ) release assay (IGRA) to detect all infected animals. A 6 h cytokine flow cytometric IFN-γ (CFC) assay was developed in effort to overcome these limitations and expand methods for studying the mechanisms of bTB immunopathogenesis. The present study was conducted to evaluate IL-1β as a biomarker to use in conjunction with the IFN-γ CFC assay to improve the diagnostic accuracy for bTB...
July 2017: Tuberculosis
https://www.readbyqxmd.com/read/28578219/identification-of-new-antigen-candidates-for-the-early-diagnosis-of-mycobacterium-avium-subsp-paratuberculosis-infection-in-goats
#2
Armel Souriau, Sandrine Freret, Benjamin Foret, Peter T J Willemsen, Douwe Bakker, Laurence A Guilloteau
Currently Mycobacterium avium subsp. paratuberculosis (MAP) infection is diagnosed through indirect tests based on the immune response induced by the infection. The antigens commonly used in IFN-γ release assays (IGRA) are purified protein derivative tuberculins (PPD). However, PPDs, lack both specificity (Sp) and sensitivity (Se) in the early phase of infection. This study investigated the potential of 16 MAP recombinant proteins and five lipids to elicit the release of IFN-γ in goats from herds with or without a history of paratuberculosis...
May 26, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28576466/are-additive-effects-of-dietary-surfactants-on-intestinal-tight-junction-integrity-an-overlooked-human-health-risk-a-mixture-study-on-caco-2-monolayers
#3
Anders Glynn, Annachiara Malin Igra, Salomon Sand, Nils Gunnar Ilbäck, Karl Erik Hellenäs, Johan Rosén, Bitte Aspenström-Fagerlund
Surfactants may cause dysfunction of intestinal tight junctions (TJs), which is a common feature of intestinal autoimmune diseases. Effects of dietary surfactants on TJ integrity, measured as trans-epithelial resistance (TEER), were studied in Caco-2 cell monolayers. Cytotoxicity was assessed as apical LDH leakage. Monolayers were apically exposed for 60 min to the dietary surfactants solanine and chaconine (SC, potato glycoalkaloids, 0-0.25 mM), perfluorooctane sulfonic acid (PFOS, industrial contaminant, 0-0...
May 30, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28543876/application-of-molecular-microbiological-and-immunological-tests-for-the-diagnosis-of-bone-and-joint-tuberculosis
#4
Yinghua Tang, Lianli Yin, Shifu Tang, Hongyu Zhang, Jihui Lan
BACKGROUND: To evaluate the application of interferon gamma release assay (IGRA), rifampicin resistant real-time fluorescence quantitative PCR technique Xpert Mycobacterium tuberculosis/rifampicin (Xpert MTB/RIF), and the levels of TNF-α and TGF-β in the diagnosis of bone and joint tuberculosis. METHODS: Eighty-six patients with bone and joint tuberculosis, diagnosed by pathology or microbiology, were examined by Xpert MTB/RIF and IGRA (T-SPOT. TB) for Mycobacterium tuberculosis infection, and the TNF-α and TGF-β levels of the patients were measured...
May 22, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28531231/utility-of-th1-cell-immune-responses-for-distinguishing-active-tuberculosis-from-non-active-tuberculosis-a-case-control-study
#5
Lifan Zhang, Xinhe Cheng, Sainan Bian, Yanhua Song, Qiang Li, Mengqiu Gao, Yueqiu Zhang, Xiaochun Shi, Xiaoqing Liu
Currently available Interferon-γ release assay (IGRA) cannot reliably differentiate active TB (ATB) from non-active TB (non-ATB). A study was performed to evaluate the value of Mycobacterium tuberculosis (MTB) specific Th1 cell immune responses which test IFN-γ and IL-2 simultaneous for differentiating ATB from non-ATB. Forty-nine newly diagnosed inpatients with ATB (26 pulmonary TB and 23 extrapulmonary TB) were enrolled as the ATB group. Forty-five volunteers with latent tuberculosis infection (LTBI) and twenty with evidence of previous TB were enrolled during the same period as the non-ATB group...
2017: PloS One
https://www.readbyqxmd.com/read/28521263/gamma-interferon-responses-to-proteome-determined-specific-recombinant-proteins-in-cattle-experimentally-and-naturally-infected-with-paratuberculosis
#6
Valerie Hughes, Jim McNair, Samuel Strain, Claire Barry, Joyce McLuckie, Mintu Nath, George Caldow, Karen Stevenson
Johne's disease (JD), is a fatal enteritis of animals caused by infection with Mycobacterium avium subspecies paratuberculosis (Map). Diagnosis of subclinical JD is problematic as test sensitivity is limited. Th1 responses to Map are activated early, thus detection of a cell-mediated response, indicated by measuring interferon gamma (IFN-γ) stimulated by mycobacterial antigens, may give the first indication of sub-clinical infection. Crude extracts of Map (PPDJ) have been used to detect the cell-mediated response in infected cattle...
April 29, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28498853/safety-and-immunogenicity-of-an-inactivated-whole-cell-tuberculosis-vaccine-booster-in-adults-primed-with-bcg-a-randomized-controlled-trial-of-dar-901
#7
C Fordham von Reyn, Timothy Lahey, Robert D Arbeit, Bernard Landry, Leway Kailani, Lisa V Adams, Brenda C Haynes, Todd Mackenzie, Wendy Wieland-Alter, Ruth I Connor, Sue Tvaroha, David A Hokey, Ann M Ginsberg, Richard Waddell
BACKGROUND: Development of a tuberculosis vaccine to boost BCG is a major international health priority. SRL172, an inactivated whole cell booster derived from a non-tuberculous mycobacterium, is the only new vaccine against tuberculosis to have demonstrated efficacy in a Phase 3 trial. In the present study we sought to determine if a three-dose series of DAR-901 manufactured from the SRL172 master cell bank by a new, scalable method was safe and immunogenic. METHODS: We performed a single site, randomized, double-blind, controlled, Phase 1 dose escalation trial of DAR-901 at Dartmouth-Hitchcock Medical Center in the United States...
2017: PloS One
https://www.readbyqxmd.com/read/28487230/assessment-of-antigenic-supplementation-of-bovine-purified-protein-derivative-for-diagnosis-of-subclinical-infection-with-mycobacterium-bovis-in-cattle
#8
A H Alvarez, A Gutiérrez-Ortega, V Gómez-Entzin, G Pérez-Mayorga, J Naranjo-Bastién, V González-Martínez, F Milián-Suazo, M Martínez-Velázquez, S Herrera-Rodríguez, E Hinojoza-Loza
Bovine tuberculosis (bTB) is usually diagnosed in vivo and ex vivo on the basis of delayed hypersensitivity reactions with a complex pool of antigens named bovine tuberculin (PPDB). The IFN-γ release assay (IGRA) for bTB is a blood-based assay that improves detection of infected cattle at early stages that escape skin testing. Improvements to IFN-γ testing with specific proteins have been performed to increase sensitivity. DosR regulon-related antigens are well known mycobacterial proteins expressed during the non-replicative phases of infection, this has been useful to improve the diagnosis of subclinical forms of TB in suspected individuals...
July 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28486818/tuberculosis-screening-among-hiv-infected-patients-tuberculin-skin-test-vs-interferon-gamma-release-assay
#9
J W Adams, C J Howe, A C Andrews, S L Allen, C Vinnard
National guidelines recommend screening for latent tuberculosis infection (LTBI) in all HIV-infected patients. Thus, the objective of this study was to measure protocol adherence to national guidelines regarding LTBI screening for HIV-infected patients entering care at an urban primary care clinic specializing in HIV care, identify clinical and other characteristics associated with adherence, and determine whether transitioning from the tuberculin skin test (TST) to the interferon-gamma release assay (IGRA) improved adherence...
May 9, 2017: AIDS Care
https://www.readbyqxmd.com/read/28482964/the-sensitivity-of-the-quantiferon-%C3%A2-tb-gold-plus-assay-in-zambian-adults-with-active-tuberculosis
#10
L Telisinghe, M Amofa-Sekyi, K Maluzi, D Kaluba-Milimo, M Cheeba-Lengwe, K Chiwele, B Kosloff, S Floyd, S-L Bailey, H Ayles
SETTING AND OBJECTIVE: To investigate the sensitivity of the new interferon-gamma release assay (IGRA), QuantiFERON®-TB Gold Plus (QFT-Plus), for active TB (used as a surrogate for latent tuberculous infection) in a Zambian TB clinic. DESIGN: Consecutive smear or Xpert® MTB/RIF-positive adult (age 18 years) pulmonary TB patients were recruited between June 2015 and March 2016. Venous blood was tested using QFT-Plus. The sensitivity was defined as the number positive divided by the total number tested...
June 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28446504/feasibility-of-ultra-high-throughput-functional-screening-of-melanoma-biopsies-for-discovery-of-novel-cancer-drug-combinations
#11
Adam A Friedman, Yun Xia, Lorenzo Trippa, Long P Le, Vivien Igras, Dennie T Frederick, Jennifer A Wargo, Kenneth K Tanabe, Donald P Lawrence, Donna S Neuberg, Keith T Flaherty, David Fisher
Purpose: Successful development of targeted therapy combinations for cancer patients depends on first discovering such combinations in predictive preclinical models. Stable cell lines and mouse xenograft models can have genetic and phenotypic drift and may take too long to generate to be useful as a personalized medicine tool. <p>Experimental Design: To overcome these limitations, we have used a platform of ultra-high-throughput functional screening of primary biopsies preserving both cancer and stroma cell populations from melanoma patients to nominate such novel combinations from a library of  thousands of drug combinations in a patient-specific manner within days of biopsy...
April 26, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28443247/evaluation-of-a-new-ifn-%C3%AE-release-assay-for-rapid-diagnosis-of-active-tuberculosis-in-a-high-incidence-setting
#12
Gen Li, Feng Li, Hui-Min Zhao, Han-Li Wen, Hai-Cong Li, Chun-Ling Li, Ping Ji, Peng Xu, Kang Wu, Zhi-Dong Hu, Shui-Hua Lu, Douglas B Lowrie, Jian-Xin Lv, Xiao-Yong Fan
Blood-based interferon-gamma (IFN-γ) release assays (IGRAs) have been proven to be useful in the diagnosis of Mycobacterium tuberculosis (Mtb) infection. However, IGRAs have not been recommended for clinical practice in most low-income settings due to cost-intensive limitations and shortage of clinical data available. The established T-SPOT. TB assay containing Mtb-specific antigens ESAT-6 and CFP10 are widely used for immunodiagonsis of Mtb infection, but the high cost is one of the restricting factors against its clinical application in the developing countries...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28438462/comparison-of-interferon-%C3%AE-release-assays-quantiferon-tb-git-and-t-spot-tb-in-renal-transplantation
#13
Satoru Ishikawa, Hidetoshi Igari, Naotake Akutsu, Mizue Tsuyuzaki, Hiromichi Aoyama, Masayuki Hasegawa, Kazunori Otsuki, Michihiro Maruyama, Kenichi Saigo, Kiminori Suzuki, Fumio Yamagishi
Renal transplant recipients are at increased risk of reactivating latent tuberculosis infection (LTBI) and progressing to active tuberculosis (TB). This study was conducted in National hospital for tuberculosis and renal transplantation (RT) in Japan. The purpose is to compare two interferon-γ release assays (IGRAs), QuantiFERON(®)-TB Gold in Tube (QFT) and T-SPOT(®).TB (TSPOT), in patients after renal transplantation for detecting latent TB infection (LTBI). Total 92 renal transplant recipients (median age 46 years, range 17-75) were prospectively enrolled, and QFT and TSPOT were concurrently examined...
April 21, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28429162/involvement-of-methylated-hbha-expressed-from-mycobacterium-smegmatis-in-an-ifn-%C3%AE-release-assay-to-aid-discrimination-between-latent-infection-and-active-tuberculosis-in-bcg-vaccinated-populations
#14
H-L Wen, C-L Li, G Li, Y-H Lu, H-C Li, T Li, H-M Zhao, K Wu, D B Lowrie, J-X Lv, S-H Lu, X-Y Fan
IFN-γ release assays (IGRAs) based on region of difference 1 (RD1) antigens have improved diagnosis of Mycobacterium tuberculosis (M. tb) infection. However, IGRAs with these antigens cannot discriminate between active tuberculosis (ATB) and latent tuberculosis infection (LTBI). M. tb heparin-binding-hemagglutinin (HBHA) induces relatively high IFN-γ responses in LTBI individuals and low responses in ATB patients, but purification of the native methylated HBHA from cultures of M. tb for immunological tests is complex and time-consuming...
April 20, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28419376/latent-tb-infection-and-pulmonary-tb-disease-among-patients-with-diabetes-mellitus-in-bandung-indonesia
#15
Raspati C Koesoemadinata, Susan M McAllister, Nanny N M Soetedjo, Dwi Febni Ratnaningsih, Rovina Ruslami, Sarah Kerry, Ayesha J Verrall, Lika Apriani, Reinout van Crevel, Bachti Alisjahbana, Philip C Hill
Background: Screening and treatment of latent TB infection (LTBI) and TB disease could reduce diabetes mellitus (DM)-associated TB. We aimed to describe the prevalence of LTBI and pulmonary TB among patients with DM in a TB-endemic setting. Methods: Patients with DM attending a hospital and community centres in Bandung, Indonesia, underwent LTBI screening using interferon gamma release assay (IGRA). TB was investigated by sputum smear, culture and x-ray. TB contacts from a parallel study were age- and sex-matched to patients with DM to compare LTBI and TB disease prevalence...
February 1, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28413722/performance-of-interferon-%C3%AE-release-assay-in-the-diagnosis-of-tuberculous-lymphadenitis-a-meta-analysis
#16
Qianqian Liu, Wenzhang Li, Yunfeng Chen, Xinmiao Du, Chengdi Wang, Binmiao Liang, Yin Tang, Yulin Feng, Chuanmin Tao, Jian-Qing He
BACKGROUND: The diagnostic values of interferon-gamma release assays (IGRA) in tuberculosis (TB) vary a lot with different site of infections, with especially higher sensitivities in chronic forms of TB such as tuberculosis of the lymph node. We conducted a meta-analysis to comprehensively evaluate the overall accuracy of diagnostic IGRA for tuberculous lymphadenitis. METHODS: Pubmed, Web of Science, EMBASE, Wanfang and CNKI databases up to February 17, 2017 were searched to identify published studies...
2017: PeerJ
https://www.readbyqxmd.com/read/28404472/the-prevalence-of-latent-tuberculosis-infection-in-rural-jiangsu-china
#17
Y Liu, S Huang, H Jiang, J Xiong, Y Wang, M Ou, J Cai, C Yang, Z Wang, S Ge, N Xia
OBJECTIVES: Diagnosis and interventional treatment of latent tuberculosis (TB) infection (LTBI) are important components in tuberculosis control. But systematic studies regarding the epidemic of LTBI are still rare in China. The objective of this study was to assess the prevalence and risk factors associated with LTBI based on the results of a domestic TB-specific gamma interferon (IFN-γ) release assay (TB-IGRA) in rural Jiangsu, China. STUDY DESIGN: Cross-sectional study of subjects registered in eight villages in Jiangsu, China...
February 4, 2017: Public Health
https://www.readbyqxmd.com/read/28387329/introducing-the-esat-6-free-igra-a-companion-diagnostic-for-tb-vaccines-based-on-esat-6
#18
Morten Ruhwald, Lena de Thurah, Davis Kuchaka, Mostafa Rafaat Zaher, Ahmed M Salman, Abdel-Rahman Abdel-Ghaffar, Faten Aly Shoukry, Sascha Wilk Michelsen, Bolette Soborg, Thomas Blauenfeldt, Stellah Mpagama, Søren T Hoff, Else Marie Agger, Ida Rosenkrands, Claus Aagard, Gibson Kibiki, Nabila El-Sheikh, Peter Andersen
There is a need for an improved vaccine for tuberculosis. ESAT-6 is a cardinal vaccine antigen with unique properties and is included in several vaccine candidates in development. ESAT-6 is also the core antigen in the IFN-γ release assays (IGRA) used to diagnose latent infection, rendering IGRA tests unspecific after vaccination. This challenge has prompted the development of a companion diagnostic for ESAT-6 based vaccines, an ESAT-6 free IGRA. We screened a panel of seven potential new diagnostic antigens not recognized in BCG vaccinated individuals...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28384350/a-dose-response-relationship-of-smoking-with-tuberculosis-infection-a-cross-sectional-study-among-21008-rural-residents-in-china
#19
Haoran Zhang, Henan Xin, Xiangwei Li, Hengjing Li, Mufei Li, Wei Lu, Liqiong Bai, Xinhua Wang, Jianmin Liu, Qi Jin, Lei Gao
OBJECTIVES: China has high burden on both of tuberculosis (TB) and tobacco use. This study aims to explore the potential link between smoking and TB infection using baseline survey data of a large-scale population-based prospective study in rural China. METHODS: Between July 1 and Sept 30, 2013, based on the baseline survey of a population-based, prospective study in rural China, the relationship between smoking and TB infection, assessed by interferon-gamma release assays (IGRA), was investigated among the total study population and only among those smokers, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28381742/the-direct-comparison-of-two-interferon-gamma-release-assays-in-the-tuberculosis-screening-of-japanese-healthcare-workers
#20
COMPARATIVE STUDY
Masaki Tanabe, Akiko Nakamura, Akie Arai, Daisuke Yamasaki, Kyoko Hirano, Tetsu Kobayashi, Osamu Taguchi, Toshihiro Kaneko, Masaaki Ito
Objective Two interferon-gamma release assays (IGRAs), the QuantiFERON-TB Gold In-Tube (QFT-GIT) and T-SPOT.TB (T-SPOT), are commercially available. The agreement between the two IGRAs in the screening of healthcare workers (HCWs) for latent tuberculosis is not well known. Methods The QFT-GIT and T-SPOT tests were performed for the baseline tuberculosis screening of 654 HCWs who worked at Mie University Hospital in Japan. The results of the two tests were directly compared. Results Nineteen (2.9%), 28 (4.3%) and 33 (5...
2017: Internal Medicine
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