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Olfatory mucosa

Hervé Cadiou, Gérard Molle
Binding of an odorant to its receptor activates the cAMP-dependent pathway, and also leads to inositol 1,4,5-trisphosphate (InsP(3)) production. This induces opening of a plasma membrane channel in olfactory receptor cells (ORCs). We investigated single-channel properties of this channel in the presence of a phospholipase C (PLC) activator (imipramine) and of a potent activator of the InsP(3)/Ca(2+) release channel (adenophostin A) by reconstituting carp olfactory cilia into planar lipid bilayers. In the presence of 53 mM barium as a charge carrier, the addition of 50 microM imipramine induced a current of 1...
May 2003: European Biophysics Journal: EBJ
V Matarazzo, A Tirard, M Renucci, J M Botto, M C Bel, J M Claverie, A Belaïch, J L Clement
Alpine Marmots (Marmota marmota) are a good model to study intraspecific chemical communication among mammals. This species has been subjected to several behavioural and biochemical studies regarding both their scent-marking behaviour by cheek-rubbing, and the chemical composition of their glandular secretions. However, no molecular study has been undertaken until today on proteins from the olfactory epithelium possibly implicated in chemical perception. In this study, we identified, to our knowledge for the first time, some olfatory receptors from this wild rodent...
November 2000: Cellular and Molecular Biology
A Yasuoka, K Endo, M Asano-Miyoshi, K Abe, Y Emori
We cloned and characterized two subfamilies of olfatory receptor (OR) genes from medaka fish (Oryzias latipes). Southern blot analysis showed that each of the two subfamilies, designated as subfamilies Y and E, consists of about five members, as usually observed for other vertebrate ORs. Analyses of the genomic clones encoding these members revealed that two members of subfamily Y and four members of subfamily E are tandemly reiterated in 15 and 22 kbp regions of the medaka genome, respectively. The members of each subfamily show very similar amino acid sequences, with similarities greater than 70%...
November 1999: Journal of Biochemistry
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