Read by QxMD icon Read

sperm movement

Yuki Katoh, Michiko Tamba, Manabu Matsuda, Kazuhiro Kikuchi, Naomichi Okamura
In order to understand the molecular mechanisms involved in the sperm capacitation, we have identified the proteins tyrosine-phosphorylated during the capacitation especially in conjunction with the regulation of the levels of reactive oxygen species (ROS) in sperm. In the present study, the effects of the tyrosine phosphorylation of cytosolic NADP + -dependent isocitrate dehydrogenase (IDPc) on its catalytic activity and on the levels of ROS in sperm have been studied. The tyrosine phosphorylated IDPc showed a significantly lowered enzymatic activity...
February 12, 2018: Biochemical and Biophysical Research Communications
Xiaorong Huang, Xiongbo Peng, Fei Xie, Wanying Mao, Hong Chen, Meng-Xiang Sun
During male gametogenesis in cereals, the generative cell undergoes a positioning process that parallels the dynamics of the central vacuole, which is believed to be associated with generative cell movement in the male gametophyte. However, the impact of the generative cell positioning and the central vacuole dynamics on male gametogenesis has remained poorly understood. Here, we report that OsGCD1 (GAMETE CELLS DEFECTIVE1) dysfunction influenced pollen development and disrupted pollen germination. Loss of function of OsGCD1 altered the central vacuole dynamics and the generative cell was mispositioned...
February 9, 2018: New Phytologist
G Bernáth, Zs Csenki, Z Bokor, L Várkonyi, J Molnár, T Szabó, Á Staszny, Á Ferincz, K Szabó, B Urbányi, L O Pap, B Csorbai
The present study investigated the effects of chilled storage and cryopreservation on ide sperm motility and fertilizing capacity alongside the longevity of sperm movement. The parameters of motility (progressive motility-pMOT, curvilinear velocity-VCL and straightness-STR) have been recorded during 48 h of chilled storage (4 °C) at 24-h intervals. The longevity of sperm movement was measured following activation for up to 120 s (in a range at 10-120 s) in freshly stripped and thawed sperm. A formerly established cryopreservation method was tested on ide sperm where motility parameters, hatching rate and larval malformation (according to 7 category groups) were investigated...
January 31, 2018: Cryobiology
Jurandir Joaquim Bernardes Júnior, Jhon Edison Jimenez, Robie Allan Bombardelli, Alex Pires de Oliveira Nuñer
Understanding the effects of environmental factors on sperm motility characteristics can increase artificial reproduction efficiency in species that do not spawn naturally in captivity, such as Megaleporinus obtusidens. This study evaluated the effects of the osmolality (25, 85, 145, 205, 265, and 325 mOsm kg-1) and composition of activating solutions (NaCl, KCl, or fructose) on the percentage of motile sperm, and the swimming velocity and path straightness of M. obtusidens spermatozoa. The concentrations of major ions in the seminal fluid were also assessed and Na+ (74...
January 29, 2018: Animal Reproduction Science
Farhad Golshan Iranpour, Khatereh Fazelian, Gholam Reza Dashti
Background: Nonoxynol-9 a nonionic surfactant is widely used for its spermicidal effects. Finding new sperm immobilizing agents is necessary because Nonoxynol-9 damages the tissues of female reproductive system. Objective: The aim of this study was to evaluate the effects of Thymoquinone (TQ) as a potential spermostatic compound on the motility and viability of human spermatozoa. Materials and Methods: In this experimental study, the effects of 5, 10, 20, 50, 100 µg/ml, 1 and 10 mg/ml of TQ on normozoospermic semen samples were investigated...
October 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
Tomoki Shojima, Feng Hou, Yusuke Takahashi, Yoshitaka Matsumura, Masahiko Okai, Akira Nakamura, Katsutoshi Mizuno, Kazuo Inaba, Masaki Kojima, Takuya Miyakawa, Masaru Tanokura
Sperm chemotaxis toward a chemoattractant is very important for the success of fertilization. Calaxin, a member of the neuronal calcium sensor protein family, directly acts on outer-arm dynein and regulates specific flagellar movement during sperm chemotaxis of ascidian, Ciona intestinalis. Here, we present the crystal structures of calaxin both in the open and closed states upon Ca2+ and Mg2+ binding. The crystal structures revealed that three of the four EF-hands of a calaxin molecule bound Ca2+ ions and that EF2 and EF3 played a critical role in the conformational transition between the open and closed states...
January 31, 2018: Scientific Reports
Chiara Gertosio, Mariasole Magistrali, Paola Musso, Cristina Meazza, Mauro Bozzola
Over the past 30 years, advances in antineoplastic treatment led to a significant increase in the survival of patients with childhood cancer. In Europe and the United States, 82% of children, adolescents, and young adults survive 5 years from the cancer diagnosis and the majority achieves long-term survival into adulthood. The impact of cancer therapy on fertility is related to the age of the patient and to the duration, dose/intensity, and type of treatment. Exposure to chemotherapy or to radiation to gonads or pituitary brings long-term complications of cancer-directed therapies that include effects on reproductive capacity...
January 18, 2018: Journal of Adolescent and Young Adult Oncology
Shizuka Koshimizu, Rumiko Kofuji, Yuko Sasaki-Sekimoto, Masahide Kikkawa, Mie Shimojima, Hiroyuki Ohta, Shuji Shigenobu, Yukiko Kabeya, Yuji Hiwatashi, Yosuke Tamada, Takashi Murata, Mitsuyasu Hasebe
MIKC classic (MIKCC)-type MADS-box genes encode transcription factors that function in various developmental processes, including angiosperm floral organ identity. Phylogenetic analyses of the MIKCC-type MADS-box family, including genes from non-flowering plants, suggest that the increased numbers of these genes in flowering plants is related to their functional divergence; however, their precise functions in non-flowering plants and their evolution throughout land plant diversification are unknown. Here, we show that MIKCC-type MADS-box genes in the moss Physcomitrella patens function in two ways to enable fertilization...
January 3, 2018: Nature Plants
Md Anisuzzaman Mondal, Yuji Takagi, Shoji A Baba, Koh-Ichi Hamano
Aim: To examine the potential of bovine follicular fluid (BFF) to attract bull spermatozoa. Methods: The ability of the BFF to attract bull sperm was evaluated by observing changes in sperm migration after being placed in a cross-column chamber. The movement parameters of the heads and flagella of the sperm that were attracted to the BFF were analyzed by using the Computer Assisted Sperm Analysis system. Results: It was observed that 61.6% of the bull sperm migrated toward the BFF when the BFF was used at a concentration of 0...
April 2017: Reproductive Medicine and Biology
Fateme Mostajer Kheirkhah, Hamid Reza Sadegh Mohammadi, Abdolhossein Shahverdi
Proper recognition and tracking of microscopic sperm cells in video images are vital steps of male infertility diagnosis and treatment. The segmentation and detection of sperms in microscopic image analysis is a complicate process as a result of their small sizes, fast movements, and considerable collisions. Histogram-based thresholding schemes are very popular for this purpose, since they are quite fast and provide almost acceptable results. This paper proposes a combined method for sperm cells detection, which consists of a non-linear pre-processing stage, a histogram-based thresholding algorithm, and a tracking method based on an adaptive distance scheme...
February 2018: Computer Methods and Programs in Biomedicine
Beata Irena Cejko, Beata Sarosiek, Sławomir Krejszeff, Radosław Kajetan Kowalski
Multiple collections of semen during the reproductive period of the common carp Cyprinus carpio L. were used to analyse changes in semen quality. Semen collection was performed on June 1 (first collection), 12 (second collection), and 19 (third collection) from individual males (n=11) by gentle abdominal massage. Semen quantity (semen volume and sperm count), quality (sperm motility and sperm viability), as well as seminal plasma parameters (pH of seminal plasma and seminal plasma osmotic pressure) and its enzymatic activity, e...
December 5, 2017: Animal Reproduction Science
G Coticchio, M Mignini Renzini, P V Novara, M Lain, E De Ponti, D Turchi, R Fadini, M Dal Canto
STUDY QUESTION: Can focused application of time-lapse microscopy (TLM) lead to a more detailed map of the morphokinetics of human fertilization, revealing novel or neglected aspects of this process? SUMMARY ANSWER: Intensive harnessing of TLM reveals novel or previously poorly characterised phenomena of fertilization, such as a cytoplasmic wave (CW) preceding pronuclear formation and kinetics of pronuclear chromatin polarization, thereby suggesting novel non-invasive biomarkers of embryo quality...
January 1, 2018: Human Reproduction
Shuang Wang, Xiongbing Zu, Longfei Liu, Juyu Tang, Xiheng Hu
To investigate the clinic value of microsurgical shunt for the treatment of varicocele combined with asthenspermia, the clinical data and therapeutic method for 3 patients, who conducted the microscope spermatic vein high ligation combined with superficial epigastric vein flow, were retrospectively analyzed. No postoperative complications were found, and the original symptoms and signs were disappeared. All patients were conducted scrotal ultrasound and semen routine after 3 months, and all indexes, including maximum internal diameter of the cord vein (erect position), sperm density, sperm survival rate, sperm deformity rate and sperm forward movement rate, were gradually improved...
October 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
Sung Woo Kim, Min Su Ki, Chan-Lan Kim, In-Sul Hwang, Ik Soo Jeon
In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash-like movement angles were compared between fresh and low-temperature-preserved (17℃ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from 11...
September 2017: Balsaeng'gwa Saengsig
Iulian Ibănescu, Claus Leiding, Heinrich Bollwein
This study aimed to identify motile sperm subpopulations in extended boar semen and to observe the presumptive seasonal variation in their distribution. Data from 4837 boar ejaculates collected over a two-year period were analyzed in terms of kinematic parameters by Computer Assisted Sperm Analysis (CASA). Individual sperm data were used to determine subgroups of motile sperm within the ejaculates using cluster analysis. Four motile sperm subpopulations (SP) were identified, with distinct movement patterns: SP1 sperm with high velocity and high linearity; SP2 sperm with high velocity but low linearity; SP3 sperm with low velocity but high linearity; and SP4 sperm with low velocity and low linearity...
October 28, 2017: Journal of Reproduction and Development
Gen L Takei, Masakatsu Fujinoki, Kaoru Yoshida, Sumio Ishijima
STUDY QUESTION: What is the role of metachronal and synchronous sliding in sperm flagellar motility? SUMMARY ANSWER: Both metachronal and oscillatory synchronous sliding are essential for sperm flagellar motility, while the change in mode of synchronous sliding between the non-oscillatory synchronous sliding of a specific pair of the doublet microtubules and the oscillatory synchronous sliding between most pairs of doublet microtubules modulates the sperm flagellar motility...
October 11, 2017: Molecular Human Reproduction
Jiwon Hong, Bridget L Dicker, Shakeela N Jayasinghe, Francesca De Gregorio, Hong Tian, Dug Yeo Han, Keith R Hudson
The vast majority of sperm are lost from the female reproductive tract in hours following natural mating or artificial insemination in mammals. Multiple complex processes including uterine contractions, mucus barriers and phagocytosis of sperm by neutrophils have been reported to be involved in the sperm loss, although the contribution of each process is uncertain. If phagocytosis by neutrophils has a significant role in sperm loss, inhibition of neutrophil response to sperm could potentially reduce the dose of sperm required for artificial insemination...
October 4, 2017: Biology of Reproduction
Jiyoung Son, Raheel Samuel, Bruce K Gale, Douglas T Carrell, James M Hotaling
Microfluidic technology has potential to separate sperm cells from unwanted debris while improving the effectiveness of assisted reproductive technologies (ART). Current clinical protocol limitations regarding the separation of sperm cells from other cells/cellular debris can lead to low sperm recovery when the sample contains a low concentration of mostly low motility sperm cells and a high concentration of unwanted cells/cellular debris, such as in semen samples from patients with pyospermia [high white blood cell (WBC) semen]...
September 2017: Biomicrofluidics
Haotian Wu, Molly S Estill, Alexander Shershebnev, Alexander Suvorov, Stephen A Krawetz, Brian W Whitcomb, Holly Dinnie, Tayyab Rahil, Cynthia K Sites, J Richard Pilsner
STUDY QUESTION: Are preconception phthalate and phthalate replacements associated with sperm differentially methylated regions (DMRs) among men undergoing IVF? SUMMARY ANSWER: Ten phthalate metabolites were associated with 131 sperm DMRs that were enriched in genes related to growth and development, cell movement and cytoskeleton structure. WHAT IS KNOWN ALREADY: Several phthalate compounds and their metabolites are known endocrine disrupting compounds and are pervasive environmental contaminants...
November 1, 2017: Human Reproduction
Tomohiro Iida, Yoko Iwata, Tatsuma Mohri, Shoji A Baba, Noritaka Hirohashi
Animal spermatozoa navigate by sensing ambient chemicals to reach the site of fertilization. Generally, such chemicals derive from the female reproductive organs or cells. Exceptionally, squid spermatozoa mutually release and perceive carbon dioxide to form clusters after ejaculation. We previously identified the pH-taxis by which each spermatozoon can execute a sharp turn, but how flagellar dynamics enable this movement remains unknown. Here, we show that initiation of the turn motion requires a swim down a steep proton gradient (a theoretical estimation of ≥0...
October 11, 2017: Scientific Reports
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"