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https://www.readbyqxmd.com/read/28274295/quality-of-pinzgau-bull-spermatozoa-following-different-periods-of-cryostorage
#1
P Chrenek, E Spaleková, L Olexikova, A Makarevich, E Kubovicova
The aim of this work was to examine the influence of cryostorage duration of Pinzgau bull's insemination doses (IDs) on some sperm traits. The IDs were frozen by a slow freezing method and stored in liquid nitrogen for different periods: less than 8 years (group 1), 8-13 years (group 2) and 14-18 years (group 3). Motility (CASA), pathological sperm rate (Giemsa staining), apoptotic (Yo-Pro-1-positive) and necrotic (propidium iodide-positive) cell occurrence and fertilizing ability (penetration/fertilization test) of spermatozoa were evaluated post-thaw...
March 9, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28261763/effects-of-selenium-nanoparticles-on-reproductive-performance-of-male-sprague-dawley-rats-at-supranutritional-and-nonlethal-levels
#2
Lujie Liu, Yudan He, Zhiping Xiao, Wenjing Tao, Jia Zhu, Bin Wang, Zixun Liu, Minqi Wang
This research investigated the influence of selenium nanoparticles (SeNPs) on the reproductive performance of male Sprague-Dawley (SD) rats. A suspension of SeNPs was consecutively administered by oral gavage for 2 weeks at supranutritional (0.2, 0.4, or 0.8 mg Se/kg bw) and nonlethal (2.0, 4.0, or 8.0 mg Se/kg bw) levels to male SD rats. The normal control (NC) rats were exposed to physiological saline alone. Biochemical parameters, sperm motility, gene expression of GPx1 and GPx4, and histopathological evaluation of male spermary were measured in this work...
March 6, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28248544/effect-of-exogenous-melatonin-treatment-on-the-reproductive-characteristics-and-progeny-of-male-rats-exposed-to-different-periods-from-light-and-darkness
#3
K Aa Majrashi, I Ah Barakat, A R Al-Himaidi, K Ag Adham
Light is an important environmental factor that controls the regulation of physiological functions of organisms. Melatonin is considered as one of the major hormones that play an important role in protecting body from many harmful effects and acts as a powerful anti-oxidant source to controlling the levels of antioxidant enzymes within the body. The main objectives of this work were to study the effect of 1) photoperiod changes and 2) exogenous melatonin hormone treatment on the sperm properties, body and testis weight, embryo characteristics, and concentration level of melatonin and testosterone in treated rat serum...
February 28, 2017: Physiological Research
https://www.readbyqxmd.com/read/28215685/effects-of-female-bovine-plasma-collected-at-different-days-of-the-estrous-cycle-on-epididymal-spermatozoa-motility
#4
M Nait Mouloud, F Ouennoughi, L Yaiche, R Kaidi, M Iguer-Ouada
The aim of this study was to assess the effects of female bovine plasma collected at different days of the reproductive cycle on epididymal spermatozoa motility and to test hypothesis that the subpopulations pattern of motile spermatozoa is affected by this treatment. Blood plasma samples were collected from five Holstein Friesian cows at different stages of the estrous cycle (days 0, 5, 10, 12 and 18), one pregnant cow and one adult bull and were diluted 1:9 (V/V) with normal saline. Female charcoal-treated plasma, Bull plasma and saline were used as controls...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28206990/a-familial-study-of-azoospermic-men-identifies-three-novel-causative-mutations-in-three-new-human-azoospermia-genes
#5
Moran Gershoni, Ron Hauser, Leah Yogev, Ofer Lehavi, Foad Azem, Haim Yavetz, Shmuel Pietrokovski, Sandra E Kleiman
PURPOSE: Up to 1% of all men experience azoospermia, a condition of complete absence of sperm in the semen. The mechanisms and genes involved in spermatogenesis are mainly studied in model organisms, and their relevance to humans is unclear because human genetic studies are very scarce. Our objective was to uncover novel human mutations and genes causing azoospermia due to testicular meiotic maturation arrest. METHODS: Affected and unaffected siblings from three families were subjected to whole-exome or whole-genome sequencing, followed by comprehensive bioinformatics analyses to identify mutations suspected to cause azoospermia...
February 16, 2017: Genetics in Medicine: Official Journal of the American College of Medical Genetics
https://www.readbyqxmd.com/read/28189122/nt-procnp-as-a-new-indicator-of-asthenozoospermia
#6
Ryszard Tomasiuk, Ricardo Faundez, Marek Cacko, Małgorzata Mikaszewska-Sokolewicz, Andrzej Cacko, Michał Rabijewski
PURPOSE: C-type natriuretic peptide (CNP) is a proinflammatory peptide. The highest concentration of CNP is found in male reproductive organs. This study aims to analyze the role of N-terminal C-type natriuretic propeptide (NT-proCNP) as a new indicator of asthenozoospermia. MATERIAL/METHODS: Semen was collected after 3-5 days of sexual abstinence from 86 men. The participants were between 25 and 38 years old, 51 of which had asthenozoospermia and 35 of which had normal sperm motility...
May 13, 2016: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28116055/the-development-of-an-intermediate-duration-tag-to-characterize-the-diving-behavior-of-large-whales
#7
Bruce R Mate, Ladd M Irvine, Daniel M Palacios
The development of high-resolution archival tag technologies has revolutionized our understanding of diving behavior in marine taxa such as sharks, turtles, and seals during their wide-ranging movements. However, similar applications for large whales have lagged behind due to the difficulty of keeping tags on the animals for extended periods of time. Here, we present a novel configuration of a transdermally attached biologging device called the Advanced Dive Behavior (ADB) tag. The ADB tag contains sensors that record hydrostatic pressure, three-axis accelerometers, magnetometers, water temperature, and light level, all sampled at 1 Hz...
January 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28101935/effect-of-cholesterol-loaded-cyclodextrins-on-cryosurvival-of-dog-spermatozoa
#8
J Khan, M Z Tahir, A Khalid, A Sattar, N Ahmad
Cryopreservation affects integrity of cholesterol and phospholipids in the plasma membrane of sperm leading to decreased fertility of frozen-thawed semen. Cholesterol-loaded cyclodextrins (CLC) have been shown to improve post-thaw semen quality in various species. The aim of this study was to investigate the optimal concentration of CLC for better post-thaw semen quality in dogs. Semen collection, through digital manipulation, was conducted once a week in four adult German shepherd dogs (n = 20 ejaculates; five ejaculates/dog)...
January 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28087654/effects-of-external-strain-on-the-regulation-of-microtubule-sliding-induced-by-outer-arm-dynein-of-sea-urchin-sperm-flagella
#9
Hiroshi Yoke, Chikako Shingyoji
Oscillatory bending movement of eukaryotic flagella is powered by orchestrated activity of dynein motor proteins that hydrolyze ATP and produce microtubule sliding. Although the ATP concentration within a flagellum is kept uniform at a few mmol l(-1) level, sliding activities of dyneins are dynamically coordinated along the flagellum in accordance with the phase of bending waves. Thus, at the organellar level the dynein not only generates force for bending but also modulates its motile activity by responding to bending of the flagellum...
January 13, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28068667/breeding-site-sampling-across-the-arctic-by-individual-males-of-a-polygynous-shorebird
#10
Bart Kempenaers, Mihai Valcu
Males of many polygynous species compete for access to fertile females without providing them with resources other than sperm and without investing in care for the offspring (male dominance polygyny). In such systems, local competition for access to females is intense and typically only a few males obtain matings, leading to strong sexual selection. Sampling multiple breeding areas could then provide a mechanism for males to increase their chances to reproduce. However, little is known about such sampling behaviour and about the spatial scale at which males compete...
January 26, 2017: Nature
https://www.readbyqxmd.com/read/28065674/mitochondria-in-teleost-spermatozoa
#11
REVIEW
Patricio Ulloa-Rodríguez, Elías Figueroa, Rommy Díaz, Manuel Lee-Estevez, Stefania Short, Jorge G Farías
There is an extraordinary diversity of reproductive modes in teleost and this variability is related to the phylogenetic relationships and adaption to very different biotopes. As in all vertebrates, sperm is produced as the end product of the process of spermatogenesis, and regarding teleost the spermatozoa lack an acrosome in almost all species and motility is activated as a response to osmolarity and ion content of the aquatic medium where the sperm is released. In this context, mitochondria possess a fundamental role for fish spermatozoa motility and integrity, hence, fertilizing potential; they are the energy supplier that allows flagellar movement and their dysfunction could play a main role in structural and functional damage to the spermatozoa...
January 5, 2017: Mitochondrion
https://www.readbyqxmd.com/read/28058736/effect-of-mitochondrial-uncoupling-and-glycolysis-inhibition-on-ram-sperm-functionality
#12
Jda Losano, Dsr Angrimani, A Dalmazzo, B R Rui, M M Brito, C M Mendes, Gkv Kawai, C I Vannucchi, Meoa Assumpção, V H Barnabe, M Nichi
Studies have demonstrated the importance of mitochondria to sperm functionality, as the main source of ATP for cellular homoeostasis and motility. However, the role of mitochondria on sperm metabolism is still controversial. Studies indicate that, for some species, glycolysis may be the main mechanism for sperm energy production. For ram sperm, such pathway is not clear. Thus, we evaluated ram sperm in response to mitochondrial uncoupling and glycolysis inhibition aiming to assess the importance of each pathway for sperm functionality...
January 6, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28054671/natriuretic-peptide-type-c-induces-sperm-attraction-for-fertilization-in-mouse
#13
Nana Kong, Xiaoting Xu, Yu Zhang, Yakun Wang, Xiaoqiong Hao, Yu Zhao, Jie Qiao, Guoliang Xia, Meijia Zhang
Mammalian spermatozoa undergo selective movement along the isthmus of the oviduct to the ampulla during ovulation, which is a prerequisite for fertilization. The factor(s) that involves in selective spermatozoa movement is still unknown. In this study, we found that the oviductal epithelium in mouse ampulla expressed high levels of natriuretic peptide type C (NPPC) in the presence of ovulated oocyte-cumulus complexes (OCCs). Spermatozoa expressed NPPC receptor natriuretic peptide receptor 2 (NPR2, a guanylyl cyclase) on the midpiece of flagellum...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28054411/peach-gum-polysaccharides-improve-the-spermatogenesis-of-kkay-mice-with-impaired-reproduction-system
#14
Li Qian, Wenjun Wang, Jie Song, Dezhong Chen
PROBLEM: Peach gum polysaccharides (PGPs) exhibit antioxidant and antibacterial activities. Nevertheless, the effect of PGPs on the spermatogenesis of KKAy mice with impaired reproduction system remains undetermined. METHOD OF STUDY: PGPs were extracted with hot water. KKAy mice were randomly divided into two groups, namely, control and PGPs (treated with 100 mg/kg PGPs). RESULTS: Oral administration of PGPs decreased the levels of serum triglyceride, total cholesterol, low-density lipoprotein cholesterol, fasting blood glucose, plasma insulin, and nitrate nitrogen level in the testes of KKAy mice...
January 5, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27995374/nuclear-migration-during-karyogamy-in-rice-zygotes-is-mediated-by-continuous-convergence-of-actin-meshwork-toward-the-egg-nucleus
#15
Yukinosuke Ohnishi, Takashi Okamoto
Fertilization is comprised of two sequential fusion processes; plasmogamy and karyogamy. Karyogamy completes with migration and fusion of the male and female nuclei in the fused cell. In animals, microtubules organized by the centrosome control female/male pronuclei migration. In contrast, the nuclear migration in fused gametes of angiosperms is controlled by actin filaments, but the mechanism that regulates actin filament-dependent nuclear migration is not clear. In this study, we prepared fused rice (Oryza sativa L...
December 19, 2016: Journal of Plant Research
https://www.readbyqxmd.com/read/27991490/two-pathways-regulate-cortical-granule-translocation-to-prevent-polyspermy-in-mouse-oocytes
#16
Liam P Cheeseman, Jérôme Boulanger, Lisa M Bond, Melina Schuh
An egg must be fertilized by a single sperm only. To prevent polyspermy, the zona pellucida, a structure that surrounds mammalian eggs, becomes impermeable upon fertilization, preventing the entry of further sperm. The structural changes in the zona upon fertilization are driven by the exocytosis of cortical granules. These translocate from the oocyte's centre to the plasma membrane during meiosis. However, very little is known about the mechanism of cortical granule translocation. Here we investigate cortical granule transport and dynamics in live mammalian oocytes by using Rab27a as a marker...
December 19, 2016: Nature Communications
https://www.readbyqxmd.com/read/27862586/effect-of-chronic-glomerulonephritis-on-the-semen-quality-and-cytokines-in-the-semen-of-infertile-males
#17
Huina Zhang, Yingfen Ying, Yilu Chen, Xiaosheng Lu, Yonggang Huang
PROBLEM: The effects of chronic glomerulonephritis (CGN) on semen quality and cytokine levels in the semen of infertile males remain undetermined. METHOD OF STUDY: Fifty-eight semen samples from normal males and CGN males with and without infertility, respectively, were analyzed. RESULTS: Semen volume, semen pH, sperm density, percentage of forward movement of sperm, sperm activate rate, sperm survival rate, and rate of normal sperm morphology of infertility males with CGN were significantly lower than those of CGN males without infertility and normal males (P<...
January 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27793367/behavioral-cognitive-and-motor-performance-and-physical-development-of-five-year-old-children-who-were-born-after-intracytoplasmic-sperm-injection-with-the-use-of-testicular-sperm
#18
Aukje M Meijerink, Liliana Ramos, Anjo J W M Janssen, Nienke M Maas-van Schaaijk, Andreas Meissner, Sjoerd Repping, Monique H Mochtar, Didi D M Braat, Kathrin Fleischer
OBJECTIVE: To evaluate at the age of 5 years the behavioral, cognitive, and motor performance and physical development of children born after testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI). DESIGN: A prospective longitudinal cohort study. SETTING: Two university medical centers. PATIENT(S): A total of 103 5-year-olds who were born after TESE-ICSI. INTERVENTION(S): The follow-up of the children was performed by questionnaires at birth and again at 1 year and at 4 years of age...
December 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27774660/the-influence-of-benign-prostatic-hyperplasia-on-sperm-morphological-features-and-sperm-dna-integrity-in-dogs
#19
R B Flores, Dsr Angrimani, B R Rui, M M Brito, R A Abreu, C I Vannucchi
Benign prostatic hyperplasia (BPH) has a high incidence in older intact dogs. Due to the increased prostatic oxidative stress and hormonal imbalance of BPH, sperm damage can arise, such as sperm morphological alterations and DNA fragmentation. This study aimed to compare the reproductive potential of healthy dogs and those affected by benign prostatic hyperplasia. Ten dogs were assigned to two experimental groups: dogs without BPH (control; n = 5) and dogs diagnosed with BPH (n = 5), based on clinical signs and ultrasonographic findings...
October 24, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27758006/mitigation-of-sperm-tail-abnormalities-using-demembranation-approach-in-the-clouded-leopard-neofelis-nebulosa
#20
W Tipkantha, P Thuwanut, B Siriaroonrat, P Comizzoli, K Chatdarong
Clouded leopards (Neofelis nebulosa) produced high proportion of abnormal spermatozoa (mainly tail defects) that can limit sperm movement and conception. The study aimed to better identify the origin of those defects using a demembranation approach. Ejaculates (1-2 ejaculations/male; n = 9) were allocated to simple washing (control; resulting in 11.7% ± 1.9% coiled tails) and processed through colloid centrifugation to reduce the number of sperm with tail defects (treatment, resulting in 5.9% ± 0.9% coiled tails)...
October 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
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