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https://www.readbyqxmd.com/read/28624941/does-static-electric-field-from-ultra-high-voltage-direct-current-transmission-lines-affect-male-reproductive-capacity-evidence-from-a-laboratory-study-on-male-mice
#1
Sixia Wu, Guoqing Di, Zhengguang Li
With the development of ultra-high-voltage direct-current (UHVDC) transmission technology and increase in transmission voltage, the issue of environmental static electric field (SEF) pollution is standing out and its possible health effects have caused much public attention. In this study, the effects of chronic exposure to SEF on reproductive capacity of male mice were investigated. Twenty Institute of Cancer Research (ICR) mice were exposed to SEF (56.3 ± 1.4 kV/m, 49 days) generated by a high-voltage device...
June 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28610652/novel-insights-into-the-role-of-cell-free-seminal-mrnas-on-semen-quality-and-cryotolerance-of-spermatozoa-in-bulls-bos-taurus
#2
Munivenkatappa Shilpa, Sellappan Selvaraju, Venkataswamy GirishKumar, Sivashanmugam Parthipan, Krishnan B Binsila, Arunachalam Arangasamy, Janivara Parameshwaraiah Ravindra
The aim of the present study was to ascertain the effectiveness of seminal plasma mRNAs as markers to assess the reproductive performance of bulls. Semen samples (33 ejaculates) from 11 bulls were evaluated for sperm kinematic and functional parameters. Total RNA was isolated from cell-free seminal (cfs) using TRIzol LS reagent and the concentration of cfs-RNA was 24.4±2.3µgmL-1 seminal plasma. The cfs-RNA was fragmented to a size of 25-500bp. Of the cfs-mRNAs screened using real time PCR, expression of protamine 1 (PRM1) was positively (P<0...
June 14, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28588746/the-stimulated-glycolytic-pathway-is-able-to-maintain-atp-levels-and-kinetic-patterns-of-bovine-epididymal-sperm-subjected-to-mitochondrial-uncoupling
#3
João D A Losano, Juan Fernando Padín, Iago Méndez-López, Daniel S R Angrimani, Antonio G García, Valquiria H Barnabe, Marcilio Nichi
Studies have reported the importance of mitochondria in sperm functionality. However, for some species, the glycolytic pathway appears to be as important as oxidative phosphorylation in ATP synthesis and sperm kinetics. These mechanisms have not been fully elucidated for bovine spermatozoa. Therefore, the aim of this study was to evaluate the role of mitochondria and the glycolytic pathway in ATP synthesis, sperm movement patterns, and oxidative homeostasis of epididymal spermatozoa in bovine specimens. We observed that mitochondrial uncoupling with protonophores significantly reduced ATP levels...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28583598/detection-of-macrophages-in-rabbit-semen-and-their-relationship-with-semen-quality
#4
Lenka Kuželová, Jaromír Vašíček, Ján Rafay, Peter Chrenek
We aimed at the evaluating the occurrence of macrophages in rabbit semen and finding possible relationship between macrophage concentration and spermatozoa quality. The concentration of macrophages in semen samples from broiler rabbit males of lines M91 and P91 (n = 30) without overt evidence of genital tract infections was determined using monocyte/macrophage lineage antigen CD14 and flow cytometry. Then the rabbits were assigned into three groups according to the macrophage concentration in semen (MΦ1 group with less than 1 × 10(6) macrophages/mL, MΦ2 group with 1...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28500503/expression-and-localization-of-myosin-vi-in-developing-mouse-spermatids
#5
Przemysław Zakrzewski, Robert Lenartowski, Maria Jolanta Rędowicz, Kathryn G Miller, Marta Lenartowska
Myosin VI (MVI) is a versatile actin-based motor protein that has been implicated in a variety of different cellular processes, including endo- and exocytic vesicle trafficking, Golgi morphology, and actin structure stabilization. A role for MVI in crucial actin-based processes involved in sperm maturation was demonstrated in Drosophila. Because of the prominence and importance of actin structures in mammalian spermiogenesis, we investigated whether MVI was associated with actin-mediated maturation events in mammals...
May 12, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28497253/effects-of-juvenile-hormone-analogue-methoprene-and-20-hydroxyecdysone-on-reproduction-in-polygonia-c-aureum-lepidoptera-nymphalidae-in-relation-to-adult-diapause
#6
Satoshi Hiroyoshi, Gadi V P Reddy, Jun Mitsuhashi
We investigated the effects of juvenile hormone analogue (methoprene) and 20-hydroxyecdysone on female and male reproduction in a nymphalid butterfly, Polygonia c-aureum. This butterfly has a facultative adult diapause controlled by the corpora allata and brain. Methoprene seems to terminate reproductive diapause, although transplantation experiments indicate that the activity of the corpora allata does not affect male mating behavior Endo (Dev Growth Differ 15:1-10, 1973a), suggesting that the brain may be involved in diapause...
May 11, 2017: Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology
https://www.readbyqxmd.com/read/28481731/posttranslational-modifications-in-spermatozoa-and-effects-on-male-fertility-and-sperm-viability
#7
Rahim Dad Brohi, Li-Jun Huo
Spermatogenesis is a complex and highly regulated process. The ability of spermatozoa to perform its function depends on multiple physiological and genetic factors that are not fully understood. Notably, due to lack of transcriptional and translational activity in spermatozoa, posttranslational modifications (PTMs) play key roles in determining their viability. PTMs not only confer structural changes in the proteome of the spermatozoa cells, but also increase the diversity of the proteome and introduce specific modifications that could be translated into functional changes in the affected spermatozoa...
May 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28455156/pentoxifylline-increase-sperm-motility-in-devitrified-spermatozoa-from-asthenozoospermic-patient-without-damage-chromatin-and-dna-integrity
#8
Ali Nabi, Mohammad Ali Khalili, Farzaneh Fesahat, Alireza Talebi, Saeed Ghasemi-Esmailabad
The freeze-thaw process results in reduced motility, viability and fertilization potential of human spermatozoa. So, a variety of substances were evaluated in order to enhance human sperm resistance to the stress of cryopreservation, such as Pentoxifylline (PTX) for improving the Intracytoplasmic sperm injection (ICSI) outcomes. The aim was to investigate the effect of PTX on sperm parameters and chromatin/DNA integrity of asthenozoospermic semen post vitrification. A total of 30 semen specimens were obtained from infertile men with asthenozoospermia...
April 25, 2017: Cryobiology
https://www.readbyqxmd.com/read/28431864/toward-an-integrative-and-predictive-sperm-quality-analysis-in-bos-taurus
#9
J L Yániz, C Soler, C Alquézar-Baeta, P Santolaria
There is a need to develop more integrative sperm quality analysis methods, enabling researchers to evaluate different parameters simultaneously cell by cell. In this work, we present a new multi-parametric fluorescent test able to discriminate different sperm subpopulations based on their labeling pattern and motility characteristics. Cryopreserved semen samples from 20 Holstein bulls were used in the study. Analyses of sperm motility using computer-assisted sperm analysis (CASA-mot), membrane integrity by acridine orange-propidium iodide combination and multi-parametric by the ISAS(®)3Fun kit, were performed...
March 31, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28425215/lipid-raft-dynamics-linked-to-sperm-competency-for-fertilization-in-mice
#10
Hitomi Watanabe, Rie Takeda, Keiji Hirota, Gen Kondoh
It is well known that mammalian sperm acquires fertilization ability after several maturation processes, particularly within the female reproductive tract. In a previous study, we found that both glycosylphosphatidylinositol (GPI)-anchored protein (GPI-AP) release and lipid raft movement occur during the sperm maturation process. In several genetic studies, release of GPI-AP is a crucial step for sperm fertilization ability in the mouse. Here, we show that lipid raft movement is also fundamental for sperm to be competent for fertilization by comparing the sperm maturation process of two mouse inbred strains, C57BL/6 and BALB/c...
May 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28424932/inverse-relationship-of-ca-2-dependent-flagellar-response-between-animal-sperm-and-prasinophyte-algae
#11
Kogiku Shiba, Kazuo Inaba
Symmetry/asymmetry conversion of eukaryotic flagellar waveform is caused by the changes in intracellular Ca(2+). Animal sperm flagella show symmetric or asymmetric waveform at lower or higher concentration of intracellular Ca(2+), respectively. In Chlamydomonas, high Ca(2+) induces conversion of flagellar waveform from asymmetric to symmetry, resulting in the backward movement. This mirror image relationship between animal sperm and Chlamydomonas could be explained by the distinct calcium sensors used to regulate the outer arm dyneins (Inaba 2015)...
April 19, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28407867/the-relationship-between-mitochondrial-dna-copy-number-and-stallion-sperm-function
#12
Christa R Darr, Luis E Moraes, Richard E Connon, Charles C Love, Sheila Teague, Dickson D Varner, Stuart A Meyers
Mitochondrial DNA (mtDNA) copy number has been utilized as a measure of sperm quality in several species including mice, dogs, and humans, and has been suggested as a potential biomarker of fertility in stallion sperm. The results of the present study extend this recent discovery using sperm samples from American Quarter Horse stallions of varying age. By determining copy number of three mitochondrial genes, cytochrome b (CYTB), NADH dehydrogenase 1 (ND1) and NADH dehydrogenase 4 (ND4), instead of a single gene, we demonstrate an improved understanding of mtDNA fate in stallion sperm mitochondria following spermatogenesis...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28395326/signaling-mechanisms-in-mammalian-sperm-motility%C3%A2
#13
Maria João Freitas, Srinivasan Vijayaraghavan, Margarida Fardilha
The goal of sperm is to fertilize the oocyte. To achieve that purpose, it must acquire motility in the epididymis and hyperactivated motility in the female reproductive tract. Motility is only achieved when the sperm presents a fully functional flagellum, is capable of producing energy to fuel the movement, and suffers epididymal maturation and capacitation. Since sperm is a transcriptionally silent cell, motility depends on the activation and/or inhibitions of key signaling pathways. This review describes and discusses the main signaling pathways involved in primary and hyperactivated motility, as well as the bioenergetic mechanisms necessary to produce energy to fuel sperm motility...
January 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28370569/aquaporin-9-is-expressed-in-the-epididymis-of-immature-and-mature-pigs
#14
B C Schimming, Cae Baumam, Pff Pinheiro, R de Matteis, R F Domeniconi
Aquaporins (AQPs) are channel proteins that facilitate the transepithelial and bidirectional movement of water. AQP9 is an aquaporin that is expressed in the mammalian epididymis. This water transport contributes to epididymal sperm concentration. This study aimed to examine the morphology of epididymal epithelium in piglets and boars, as well as the expression and immunolocalization of AQP9. The piglets presented an epididymal epithelium in differentiation with principal, basal and apical cells. The cellular population of the epididymal epithelium in boars consisted of principal, basal, apical, clear and narrow cells...
March 30, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28345486/objective-analysis-of-vaginal-ultrasound-video-clips-for-exploring-uterine-peristalsis-post-vaginal-and-cesarean-section-deliveries
#15
Silvia Gora, David Elad, Ariel J Jaffa
The nonpregnant uterus is characterized by cyclic contractions that assist in sperm transport to the fallopian tube, embryo transport to implantation site, and expulsion of menstrual debris. The effect of post-Cesarean section (CS) scar on uterine peristalsis is unclear, while worldwide the prevalence of CS deliveries is increasing. In this study, we developed a new objective method for analysis of dynamic characteristics of the nonpregnant uterus from transvaginal ultrasound (TVUS) recordings when the uterine cavity is not clearly observed, as may be the case in post-CS uteri...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28344405/biochemical-components-of-seminal-plasma-and-their-correlation-to-the-fresh-seminal-characteristics-in-marwari-stallions-and-poitou-jacks
#16
Thirumala Rao Talluri, Gorakh Mal, Sanjay Kumar Ravi
AIM: To investigate various biochemical components of seminal plasma in Marwari stallions and Poitou Jacks and to find out their correlation with that of the seminal characteristics. MATERIALS AND METHODS: In this study, semen was collected from six Marwari stallions and six Poitou jacks aged from 4 to 6 years and with known fertility status. The semen collection from the stallions were collected during the breeding season, i.e., between the months of April and June...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28331090/hili-inhibits-hiv-replication-in-activated-t-cells
#17
B Matija Peterlin, Pingyang Liu, Xiaoyun Wang, Daniele Cary, Wei Shao, Marie Leoz, Tian Hong, Tao Pan, Koh Fujinaga
P-element-induced wimpy-like (Piwil) proteins restrict the replication of mobile genetic elements in the germ line. They are also expressed in many transformed cell lines. In this study, we discovered that the human Piwil 2 (Hili) protein can also inhibit HIV replication, especially in activated CD4(+) T cells that are the preferred target cells for this virus in the infected host. Although resting cells did not express Hili, its expression was rapidly induced following T cell activation. In these cells and transformed cell lines, depletion of Hili increased levels of viral proteins and new viral particles...
June 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28274295/quality-of-pinzgau-bull-spermatozoa-following-different-periods-of-cryostorage
#18
P Chrenek, E Spaleková, L Olexikova, A Makarevich, E Kubovicova
The aim of this work was to examine the influence of cryostorage duration of Pinzgau bull's insemination doses (IDs) on some sperm traits. The IDs were frozen by a slow freezing method and stored in liquid nitrogen for different periods: less than 8 years (group 1), 8-13 years (group 2) and 14-18 years (group 3). Motility (CASA), pathological sperm rate (Giemsa staining), apoptotic (Yo-Pro-1-positive) and necrotic (propidium iodide-positive) cell occurrence and fertilizing ability (penetration/fertilization test) of spermatozoa were evaluated post-thaw...
March 9, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28261763/effects-of-selenium-nanoparticles-on-reproductive-performance-of-male-sprague-dawley-rats-at-supranutritional-and-nonlethal-levels
#19
Lujie Liu, Yudan He, Zhiping Xiao, Wenjing Tao, Jia Zhu, Bin Wang, Zixun Liu, Minqi Wang
This research investigated the influence of selenium nanoparticles (SeNPs) on the reproductive performance of male Sprague-Dawley (SD) rats. A suspension of SeNPs was consecutively administered by oral gavage for 2 weeks at supranutritional (0.2, 0.4, or 0.8 mg Se/kg bw) and nonlethal (2.0, 4.0, or 8.0 mg Se/kg bw) levels to male SD rats. The normal control (NC) rats were exposed to physiological saline alone. Biochemical parameters, sperm motility, gene expression of GPx1 and GPx4, and histopathological evaluation of male spermary were measured in this work...
March 6, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28248544/effect-of-exogenous-melatonin-treatment-on-the-reproductive-characteristics-and-progeny-of-male-rats-exposed-to-different-periods-from-light-and-darkness
#20
K Aa Majrashi, I Ah Barakat, A R Al-Himaidi, K Ag Adham
Light is an important environmental factor that controls the regulation of physiological functions of organisms. Melatonin is considered as one of the major hormones that play an important role in protecting body from many harmful effects and acts as a powerful anti-oxidant source to controlling the levels of antioxidant enzymes within the body. The main objectives of this work were to study the effect of 1) photoperiod changes and 2) exogenous melatonin hormone treatment on the sperm properties, body and testis weight, embryo characteristics, and concentration level of melatonin and testosterone in treated rat serum...
February 28, 2017: Physiological Research
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