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https://www.readbyqxmd.com/read/29352770/concentration-dependent-effect-of-bleaching-agents-on-the-immunolabeling-of-interleukin-6-interleukin-17-and-cd5-positive-cells-in-the-dental-pulp
#1
F Benetti, J E Gomes-Filho, L L Ferreira, G Sivieri-Araújo, E Ervolino, A L F Briso, L T A Cintra
AIM: To evaluate lymphocyte-like cell activation (CD5-positive cells) and the expression of interleukin (IL)-6 and IL-17 in the pulp after dental bleaching with two concentrations of hydrogen peroxide (H2 O2 ). METHODOLOGY: The right and left maxillary molars from 40 rats were treated randomly with bleaching gel with 20% H2 O2 (BLUE group, 1 application of 50 min), 35% H2 O2 (MAXX group, 3 applications of 15 min), or placebo gel (Control). After 2 and 30 days, the rats were killed (n=10), and the jaws were processed for histological and immunohistochemistry analysis of the pulp tissue...
January 20, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29341308/stiffness-regulates-the-proliferation-and-osteogenic-odontogenic-differentiation-of-human-dental-pulp-stem-cells-via-the-wnt-signalling-pathway
#2
Nanxin Liu, Mi Zhou, Qi Zhang, Tao Zhang, Taoran Tian, Quanquan Ma, Changyue Xue, Shiyu Lin, Xiaoxiao Cai
OBJECTIVES: Researches showed that stiffness of the extracellular matrix can affect the differentiation of many stem cells. Dental pulp stem cells (DPSCs) are a promising type of adult stem cell. However, we know little about whether and how the behaviour of DPSCs is influenced by stiffness. MATERIALS AND METHODS: We carried out a study that cultured DPSCs on tunable elasticity polydimethylsiloxane substrates to investigate the influence on morphology, proliferation, osteogenic/odontogenic differentiation and its possible mechanism...
January 17, 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29336883/long-term-prognosis-of-pulpal-status-of-traumatized-teeth-exhibiting-contradictory-results-between-pulp-sensibility-test-and-ultrasound-doppler-flowmetry-a-retrospective-study
#3
So-Yeon Ahn, Dohyun Kim, Sung-Ho Park
INTRODUCTION: In this retrospective study, we investigated long-term (over 3 years) follow-up results of teeth that exhibited contradictory results between the pulp sensibility test (thermal or electric pulp test) and ultrasound Doppler flowmetry (UDF) until 1 year after trauma to inspect the prognosis of the pulp. METHODS: Data were collected from the records of trauma patients in our hospital between February 2012 and May 2015. The teeth that had continuously shown contrasting results on the pulp sensibility test and UDF until 1 year after trauma were chosen for the study...
January 12, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29327447/itraq-based-proteomic-analysis-explore-the-influence-of-hypoxia-on-the-proteome-of-dental-pulp-stem-cells-under-3d-culture
#4
Lei Dou, Qifang Yan, Panpan Liang, Pengfei Zhou, Yan Zhang, Ping Ji
Various approaches are used to improve the efficacy of mesenchymal stem cells(MSCs) therapy. Hypoxic preconditioning is one of the most commonly-applied strategy prior to MSCs transplantation for enhancing the therapeutic effect. Identifying the effect of hypoxic preconditioning is helpful for better application. However, the influence of hypoxia on dental tissue derived MSCs cultured in 3D was lacking. Thus, this present study is to investigate the gene expression and proteome of dental pulp stem cells(DPSCs) after hypoxic preconditioning...
January 12, 2018: Proteomics
https://www.readbyqxmd.com/read/29324319/dental-age-estimation-employing-cbct-scans-enhanced-with-mimics-software-comparison-of-two-different-approaches-using-pulp-tooth-volumetric-analysis
#5
Muhammad Khan Asif, Phrabhakaran Nambiar, Shani Ann Mani, Norliza Binti Ibrahim, Iqra Muhammad Khan, Prema Sukumaran
The methods of dental age estimation and identification of unknown deceased individuals are evolving with the introduction of advanced innovative imaging technologies in forensic investigations. However, assessing small structures like root canal volumes can be challenging in spite of using highly advanced technology. The aim of the study was to investigate which amongst the two methods of volumetric analysis of maxillary central incisors displayed higher strength of correlation between chronological age and pulp/tooth volume ratio for Malaysian adults...
January 4, 2018: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/29322655/efficiency-of-er-yag-laser-in-debonding-of-porcelain-laminate-veneers-by-contact-and-non-contact-laser-application-modes-in-vitro-study
#6
Mohand ALBalkhi, Eyad Swed, Omar Hamadah
OBJECTIVE: To determine the efficiency of debonding porcelain laminate veneers (PLV) by using several laser parameters and two different application modes of Er:YAG laser [contact (CM) and non-contact (NCM)], by verification of the consumed PLV debonding time and the changes in dental pulp temperature. MATERIALS AND METHODS: Forty extracted non-carious human maxillary premolars were prepared for receiving PLV. Sixteen of them were divided into two groups, each of them comprised eight samples based on the application mode; group A with NCM, and group B with CM...
January 11, 2018: Journal of Esthetic and Restorative Dentistry
https://www.readbyqxmd.com/read/29319894/antibiotic-prescribing-for-endodontic-therapies-a-comparative-survey-between-general-dental-practitioners-gdps-and-final-year-bachelor-of-dental-surgery-bds-students-in-cardiff-uk
#7
A A Al Masan, P M H Dummer, D J J Farnell, M E Vianna
AIM: To evaluate the views of final-year dental surgery (BDS; G1) students at Cardiff University and general dental practitioners (GDPs; G2) within the city of Cardiff, Wales on antibiotic prescribing for endodontic conditions, and investigate the potential differences between the two groups. METHODS: A cross-sectional online questionnaire-based survey of 12 qualitative and quantitative questions was distributed to 76 final-year BDS Cardiff University students and 55 dental practices within Cardiff, UK...
January 10, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29316874/liposomal-delivery-of-demineralised-dentine-matrix-for-dental-tissue-regeneration
#8
Genevieve Elizabeth Melling, John Colombo, Steven J Avery, Wayne Ayre, Samuel L Evans, Rachel Jane Waddington, Alastair James Sloan
Current dental restorations have short longevity, consequently there is a need for novel tissue engineering strategies that aim to regenerate the dentine-pulp complex. Dentine matrix contains a myriad of bioactive growth factors and extracellular matrix proteins associated with the recruitment, proliferation and differentiation of dental pulp progenitor cells. Here, we show that demineralised dentine matrix (DDM), from non-carious dentine, can be encapsulated into liposomes for delivery to dental tissue to promote regeneration...
January 9, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29313816/osteopontin-regulates-dentin-and-alveolar-bone-development-and-mineralization
#9
B L Foster, M Ao, C R Salmon, M B Chavez, T N Kolli, A B Tran, E Y Chu, K R Kantovitz, M Yadav, S Narisawa, J L Millán, F H Nociti, M J Somerman
The periodontal complex is essential for tooth attachment and function and includes the mineralized tissues, cementum and alveolar bone, separated by the unmineralized periodontal ligament (PDL). To gain insights into factors regulating cementum-PDL and bone-PDL borders and protecting against ectopic calcification within the PDL, we employed a proteomic approach to analyze PDL tissue from progressive ankylosis knock-out (Ank-/-) mice, featuring reduced PPi, rapid cementogenesis, and excessive acellular cementum...
December 4, 2017: Bone
https://www.readbyqxmd.com/read/29313241/cd146-positive-human-dental-pulp-stem-cells-promote-regeneration-of-dentin-pulp-like-structures
#10
Mikiko Matsui, Tomoko Kobayashi, Takeo W Tsutsui
CD146 and STRO-1 are endothelial biomarkers that are co-expressed on the cellular membranes of blood vessels within human dental pulp tissue. This study characterized the percentage of dentin-like structures produced by CD146-positive (CD146+) human dental pulp stem cells (DPSCs), compared with their CD146-negative (CD146-) counterparts. DPSC populations were enriched using magnetic-activated cell sorting (MACS), yielding CD146+ and CD146- cells, as well as mixtures composed of 25% CD146+ cells and 75% CD146- cells (CD146+/-)...
January 8, 2018: Human Cell
https://www.readbyqxmd.com/read/29308523/-use-of-visual-analogue-scale-and-pain-related-factors-to-evaluate-the-impact-of-different-orthodontic-forces-on-human-dental-pulp-in-patients-who-underwent-fixed-dental-treatment
#11
Wan-Hong Chen, Shi-Xiong Cai, Yu-Huan Sun
PURPOSE: To investigate the role of visual analogue scale (VAS) and pain-related factors, like tumor necrosis factor-alpha (TNF-α), interleukin-8 (IL- 8), interleukin-6 (IL-6) and interleukin-1 beta (IL-1β) in evaluation of the impact of different orthodontic forces on human dental pulp in patients who underwent fixed dental treatment. METHODS: From January 2014 to April 2015, 156 patients with maxillary bilateral premolar treated with tooth fixed were randomly divided into 4 groups, 42 patients in control group, 40 patients in 100 g-force group, 40 patients in 150 g-force group, and 39 in 200 g-force group...
October 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29308519/-influence-of-age-on-the-effectiveness-of-revascularization-in-immature-permanent-teeth
#12
Wen-Feng Cai, Song Li, Ling Xie, Li-Li Yao, Chun-Hong Wang, Zhong-Hong Ren
PURPOSE: To evaluate the effect of age on the potential of dental pulp regeneration in young permanent teeth with periapical periodontitis. METHODS: A total of 30 mandibular premolars from 9-18 years old patients with pulp necrosis were divided into 2 groups, group A (younger age group): 9-13 years old, and group B (older age group): 14-18 years old. Revascularization procedures were performed for all patients. Follow-up was done for up to 18 months. Standardized radiographs of cone-beam CT (CBCT) were digitally evaluated for increase in root length and thickness...
October 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29308506/-mirna-profile-of-the-human-dental-pulp-cells-during-odontoblast-differentiation-induced-by-bmp-2
#13
Li-Rong Bao, Wen-Qing Zhao, Tian Lin, Yan-Ling Lu, Yu Wu
PURPOSE: To screen and verify the differentially expressed microRNAs (miRNAs) during the differentiation of human dental pulp cells (hDPCs) to odontoblasts induced by BMP-2. METHODS: The isolated hDPCs were cultured in vitro and induced by BMP-2. The levels of ALP, DMP-1 and DSPP were quantified by quantitative real-time polymerase chain reaction (qRT-PCR). The potential characteristics of hDPCs were investigated by miRNA microarray and highly expressed miRNAs were selected with bio-information software for predicting target genes and their biological functions...
October 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29306537/metformin-enhances-the-differentiation-of-dental-pulp-cells-into-odontoblasts-by-activating-ampk-signaling
#14
Wei Qin, Xianling Gao, Tao Ma, Michael D Weir, Jing Zou, Bing Song, Zhengmei Lin, Abraham Schneider, Hockin H K Xu
INTRODUCTION: Metformin is a first-line drug for treating type 2 diabetes that regulates the differentiation of mesenchymal stem cells. Its effects on human dental pulp cells (DPCs) remain unknown. This study aimed to investigate the effects of metformin on the proliferation and differentiation of DPCs. METHODS: A live/dead viability assay kit was used to examine the effects of metformin on the cell viability of DPCs. Cell proliferation was analyzed using a cell counting kit (CCK-8; Dojindo, Tokyo, Japan)...
January 3, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29306073/mir-143-suppresses-the-osteogenic-differentiation-of-dental-pulp-stem-cells-by-inactivation-of-nf-%C3%AE%C2%BAb-signaling-pathway-via-targeting-tnf-%C3%AE
#15
Peng Zhang, Wenli Yang, Guofang Wang, Yajing Li
BACKGROUND: Dental pulp stem cells (DPSCs) are multipotent and play an important role in repairing damaged and/or defective dentinogenesis/osteogenesis. Recent studies have documented the implication of miR-143 in osteogenic differentiation of DPSCs. Nevertheless, the detailed mechanisms of miR-143 involved in the osteogenic differentiation of DPSCs remain to be further elaborated. METHODS: Isolated DPSCs were incubated with osteogenic differentiation medium to induce osteogenic differentiation...
December 30, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29304423/pulpal-blood-flow-recorded-from-exposed-dentine-with-a-laser-doppler-flow-meter-using-red-or-infrared-light
#16
Kanittha Kijsamanmith, Noppakun Vongsavan, Bruce Matthews
OBJECTIVE: To determine the percentage of the blood flow signal that is derived from dental pulp when recording from exposed dentine in a human premolar. DESIGN: Recordings were made from 7 healthy teeth in 5 subjects (aged 22-33 yr.) with a laser Doppler flow meter (Periflux 4001) using either a red (635 nm) or an infrared (780 nm) laser. After exposing dentine above the buccal pulpal horn (cavity diam. 1.6 mm, depth 3 mm) and isolating the crown with opaque rubber dam, blood flow was recorded alternately with infrared or red light from the exposed dentine under four conditions: before and after injecting local anaesthetic (3% Mepivacaine without vasoconstrictor) (LA) over the apex of the root of the tooth; after exposing the pulp by cutting a buccal, class V cavity in the tooth; and after sectioning the coronal pulp transversely through the exposure...
December 16, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29303122/microarray-analysis-of-the-gene-expression-profile-in-triethylene-glycol-dimethacrylate-treated-human-dental-pulp-cells
#17
D Torun, Z Ö Torun, K Demirkaya, M Sarper, M P Elçi, F Avcu
OBJECTIVE: Triethylene glycol dimethacrylate (TEGDMA) is an important resin monomer commonly used in the structure of dental restorative materials. Recent studies have shown that unpolymerized resin monomers may be released into the oral environment and cause harmful biological effects. We investigated changes in the gene expression profiles of TEGDMA-treated human dental pulp cells (hDPCs) following short- (1-day) and long-term (7-days) exposure. MATERIALS AND METHODS: HDPCs were exposed to a noncytotoxic concentration of TEGDMA, and gene expression profiles were evaluated by microarray analysis...
November 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29302286/management-of-permanent-teeth-with-necrotic-pulps-and-open-apices-according-to-the-stage-of-root-development
#18
Hugo Plascencia, Mariana Díaz, Gerardo Gascón, Susana Garduño, Carlos Guerrero-Bobadilla, Salvador Márquez-De Alba, Geovani González-Barba
Background: This paper analyzed the distribution of treatments for permanent teeth with necrotic pulps and open apices according to the stage of root development. Material and Methods: Dental records from all root canal procedures performed in permanent teeth with necrotic pulps and open apices over a period of 14 years by residents of the Speciality of Endodontics, University of Guadalajara, Mexico, were analized. Results: Records from 206 treatments were mainly divided into the following 3 different stages according to criteria described by Cvek: stage IV (n = 79, 38...
November 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/29298406/a-miniature-swine-model-for-stem-cell-based-de-novo-regeneration-of-dental-pulp-and-dentin-like-tissue
#19
Xiaofei Zhu, Jie Liu, Zongdong Yu, Chao-An Chen, Hacer Aksel, Adham A Azim, George T-J Huang
The goal of this study was to establish mini-swine as a large animal model for stem cell-based pulp regeneration studies. Swine dental pulp stem cells (sDPSCs) were isolated from mini-swine and characterized in vitro. For in vivo studies, we first employed both ectopic and semi-orthotopic study models using severe combined immunodeficiency mice. One is hydroxyapatite-tricalcium phosphate (HA/TCP) model for pulp-dentin complex formation, and the other is tooth fragment model for complete pulp regeneration with new dentin depositing along the canal walls...
January 3, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29298403/intrapulpal-temperature-increases-caused-by-445-nm-diode-laser-assisted-debonding-of-self-ligating-ceramic-brackets-during-simulated-pulpal-fluid-circulation
#20
Steffen Stein, Johannes Wenzler, Andreas Hellak, Michael Schauseil, Heike Korbmacher-Steiner, Andreas Braun
OBJECTIVE: This study investigated temperature increases in dental pulp resulting from laser-assisted debonding of ceramic brackets using a 445-nm diode laser. MATERIALS AND METHODS: Eighteen ceramic brackets were bonded in standardized manner to 18 caries-free human third molars. Pulpal fluid circulation was simulated by pumping distilled water at 37°C through the pulp chamber. The brackets were irradiated with a 445-nm diode laser. Temperatures were measured using a thermal camera at points P1 (center of the pulp) and P2 (in the hard dental tissue) at the baseline (T0), at the start and end of laser application (T1 and T2), and the maximum during the sequence (Tmax)...
January 3, 2018: Photomedicine and Laser Surgery
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