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https://www.readbyqxmd.com/read/29159326/overexpression-of-id1-reverses-the-repression-of-human-dental-pulp-stem-cells-differentiation-induced-by-twist1-silencing
#1
Izabela Maciejewska, Monika Sakowicz-Burkiewicz, Marta Krzeminska, Tadeusz Pawelczyk
Multiple studies showed that the cessation of TWIST1 expression is the prerequisite for osteoblasts' maturation. However, recent reports revealed that the function of TWIST1 is different in the dental pulp stem cells (DPSCs), where a high level of TWIST1 expression promoted DPSCs' differentiation. The aim of the study was to investigate the impact of TWIST1 and ID1 on the differentiation process in the human DPSCs. METHODS: TWIST1 and ID1 expression in the DSPCs was modulated by lentivirus transduction...
November 20, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/29155305/hey1-and-hey2-are-differently-expressed-during-mouse-tooth-development
#2
Kotono Kibe, Mitsushiro Nakatomi, Shinji Kataoka, Takashi Toyono, Yuji Seta
The Hey family (also known as Chf, Herp, Hesr, and Hrt) is a set of Hairy/Enhancer of Split-related basic helix-loop-helix type transcription factors. Hey1, Hey2, and HeyL have been identified in mammals. Although Hey proteins are known to regulate cardiovascular development, muscle homeostasis, osteogenesis, neurogenesis, and oncogenesis, their roles in tooth development have been largely obscure. Therefore, this study aimed to clarify detailed spatiotemporal expression patterns of Hey1 and Hey2 in developing molars and incisors of mice by section in situ hybridization...
November 16, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29150644/therapeutic-effects-of-hepatocyte-growth-factor-overexpressing-dental-pulp-stem-cells-on-liver-cirrhosis-in-a-rat-model
#3
Xiao-Fang Cao, Shi-Zhu Jin, Liang Sun, Yuan-Bo Zhan, Feng Lin, Ying Li, Ying-Lian Zhou, Xiu-Mei Wang, Li Gao, Bin Zhang
Cirrhosis is the terminal stage of hepatic diseases and is prone to develop into hepatocyte carcinoma. Increasing evidence suggests that the transplantation of dental pulp stem cells (DPSCs) may promote recovery from cirrhosis, but the key regulatory mechanisms involved remain to be determined. In this study, we overexpressed human hepatocyte growth factor (hHGF) in primary rat DPSCs and evaluated the effects of HGF overexpression on the biological behaviors and therapeutic efficacy of grafted DPSCs in cirrhosis...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29149082/the-biological-properties-of-ogi-surfaces-positively-act-on-osteogenic-and-angiogenic-commitment-of-mesenchymal-stem-cells
#4
Paolo Ghensi, Eriberto Bressan, Chiara Gardin, Letizia Ferroni, Maria Costanza Soldini, Federico Mandelli, Claudio Soldini, Barbara Zavan
Osteogenesis process displays a fundamental role during dental implant osteointegration. In the present work, we studied the influence of Osteon Growth Induction (OGI) surface properties on the angiogenic and osteogenic behaviors of Mesenchymal Stem cells (MSC). MSC derived from dental pulp and HUVEC (Human Umbilical Vein Endothelial Cells) were grown in on OGI titanium surfaces, and cell proliferation and DNA synthesis were evaluated by MTT [3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] test and DNA quantification...
November 17, 2017: Materials
https://www.readbyqxmd.com/read/29143185/osteogenic-prospective-of-deriving-human-dental-stem-cells-in-collagen-matrix-boost
#5
Tong-Jing Fang, Ding-Han Wang, Chia-Yu Wang, Raju Poongodi, Nien-Hsien Liou, Jiang-Chuan Liu, Ming-Lun Hsu, Po-Da Hong, Shih-Fang Yang, Meng-Lun Liu
Stem cells derived from oral tissue represent a highly attractive alternative source for clinical bone regeneration because they can be collected by non-invasive or minimally invasive procedures. Herein, we describe the human dental stem cells (DSCs) deriving from buccal fat pads (BFP), dental pulp (DP) of impacted teeth, and periodontal ligaments (PDL) to obtain BFPSCs, DPSCs, and PDLSCs, respectively. Cells were purified with selected medium and expanded through passages in stem cell culture medium. Purified cells were characterized for stemness by their growth rate, immunostaining, and multilineage differentiation ability...
November 15, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29139614/human-dental-stem-cell-derived-transgene-free-ipscs-generate-functional-neurons-via-embryoid-body-mediated-and-direct-induction-methods
#6
Ikbale El Ayachi, Jun Zhang, Xiao-Ying Zou, Zongdong Yu, Wei Wei, Kristen M S O'Connell, George T-J Huang
Induced pluripotent stem cells (iPSCs) give rise to neural stem/progenitor cells (NSCs), serving as a good source for neural regeneration. Here, we established transgene-free (TF) iPSCs from dental stem cells (DSCs) and determined their capacity to differentiate into functional neurons in vitro. Generated TF iPSCs from stem cells of apical papilla (SCAP) and dental pulp stem cells (DPSCs) underwent two methods -- embryoid body (EB)-mediated and direct induction, to guide TF-DSC iPSCs along with H9 or H9 Syn-GFP (human embryonic stem cells) into functional neurons in vitro...
November 15, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29139168/hunter-gatherer-dental-pathology-do-historic-accounts-of-aboriginal-australians-correspond-to-the-archeological-record-of-dental-disease
#7
Judith Littleton
OBJECTIVES: Studies of hunter-gatherer oral pathology, particularly in Australia, often focus upon dental wear and caries or assume that historic studies of Aboriginal people reflect the precontact past. Consequently the range of population variation has been underestimated. In this paper dental pathology from human remains from Roonka are compared with a model of dental pathology derived from historic studies. The aim is to identify aspects of dental pathology indicative of regional or intra-population diversity...
November 15, 2017: American Journal of Human Biology: the Official Journal of the Human Biology Council
https://www.readbyqxmd.com/read/29138952/age-estimation-by-assessment-of-pulp-chamber-volume-a-bayesian-network-for-the-evaluation-of-dental-evidence
#8
Emanuele Sironi, Franco Taroni, Claudio Baldinotti, Cosimo Nardi, Gian-Aristide Norelli, Matteo Gallidabino, Vilma Pinchi
PURPOSE: The present study aimed to investigate the performance of a Bayesian method in the evaluation of dental age-related evidence collected by means of a geometrical approximation procedure of the pulp chamber volume. Measurement of this volume was based on three-dimensional cone beam computed tomography images. METHODS: The Bayesian method was applied by means of a probabilistic graphical model, namely a Bayesian network. Performance of that method was investigated in terms of accuracy and bias of the decisional outcomes...
November 14, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/29138868/hdac-inhibitor-lmk%C3%A2-235-promotes-the-odontoblast-differentiation-of-dental-pulp-cells
#9
Zhao Liu, Ting Chen, Qianqian Han, Ming Chen, Jie You, Fuchun Fang, Ling Peng, Buling Wu
The role of dental pulp cells (DPCs) in hard dental tissue regeneration had received increasing attention because DPCs can differentiate into odontoblasts and other tissue‑specific cells. In recent years, epigenetic modifications had been identified to serve an important role in cell differentiation, and histone deacetylase (HDAC) inhibitors have been widely studied by many researchers. However, the effects of HDAC4 and HDAC5 on the differentiation of DPCs and the precise molecular mechanisms remain unclear...
November 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29138798/hey1-functions-as-a-positive-regulator-of-odontogenic-differentiation-in-odontoblast%C3%A2-lineage-cells
#10
Xiao Yin, Zhaobin Zeng, Jinliang Xing, Ansheng Zhang, Wenkai Jiang, Wei Wang, Hantang Sun, Longxing Ni
Substantial evidence has indicated that Notch and bone morphogenetic protein (BMP) signaling may regulate odontoblastic differentiation. Hairy/enhancer‑of‑split related with YRPW motif 1 (Hey1), a downstream target gene of Notch and BMP signaling, is expressed in dental pulp tissues and has been demonstrated to be responsible for osteoblast mineralization. The aim of this study was to investigate the effects of Hey1 on odontoblast differentiation. The results of the study demonstrated that Hey1 expression in odontoblast‑lineage cells (OLCs) was upregulated by stimulation of osteoblastic/odontoblastic differentiation medium containing ascorbic acid, β‑glycerol phosphate and dexamethasone...
November 13, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29132068/secretoneurin-and-pe-11-immunoreactivity-in-the-human-dental-pulp
#11
René Steiner, Reiner Fischer-Colbrie, Athanasia Bletsa, Johannes Laimer, Josef Troger
OBJECTIVE: To explore whether there are differences in the concentration of the secretogranin II-derived peptide secretoneurin and the chromogranin B-derived peptide PE-11 between the healthy and inflamed human dental pulps. Furthermore, colocalization studies with calcitonin gene-related peptide were performed to confirm the sensory origin of the peptidergic nerves in the dental pulp. DESIGN: The concentrations of secretoneurin and PE-11 were determined by highly sensitive radioimmunoassays in extracts of dental pulps, the molecular form of secretoneurin immunoreactivities by RP-HPLC with subsequent radioimmunoassay and colocalization studies with calcitonin gene-related peptide were performed by double immunofluorescence...
November 9, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29131087/deferoxamine-induced-migration-and-odontoblast-differentiation-via-ros-dependent-autophagy-in-dental-pulp-stem-cells
#12
Xuan Wang, Tian Tian Wu, Long Jiang, Du Rong, Ya Qin Zhu
BACKGROUND/AIMS: As a vital degradation and recycling system, autophagy plays an essential role in regulating the differentiation of stem cells. We previously showed that iron chelator deferoxamine (DFO) could promote the repair ability of dental pulp stem cells (DPSCs). Here, we investigated the effect of DFO in autophagy and the role of autophagy in regulating the migration and odontoblast differentiation of DPSCs. METHODS: Transmission electron microscopy, immunofluorescence staining and western blotting were performed to evaluate the autophagic activity of DPSCs...
November 1, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29130547/mir-140-5p-mediated-regulation-of-the-proliferation-and-differentiation-of-human-dental-pulp-stem-cells-occurs-through-the-lipopolysaccharide-toll-like-receptor-4-signaling-pathway
#13
De-Gang Sun, Bing-Chang Xin, Di Wu, Lei Zhou, Hong-Bin Wu, Wen Gong, Jian Lv
Human dental pulp stem cells (DPSCs) are oral mesenchymal stem cells with potential to differentiate into various cell types. Recent studies of DPSCs have focused on microRNAs (miRNAs), a class of small noncoding RNAs that play crucial roles in regulating DPSC phenotypes. In the current study, the expression of miR-140-5p was significantly decreased during lipopolysaccharide (LPS)-mediated differentiation of DPSCs in vitro. Overexpression of miR-140-5p enhanced proliferation of DPSCs and inhibited DPSC differentiation, whereas suppression of miR-140-5p produced the opposite effect...
December 2017: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/29130364/fluorescent-labeling-and-2-photon-imaging-of-mouse-tooth-pulp-nociceptors
#14
A Kadala, P Sotelo-Hitschfeld, Z Ahmad, P Tripal, B Schmid, A Mueller, L Bernal, Z Winter, S Brauchi, U Lohbauer, K Messlinger, J K Lennerz, K Zimmermann
Retrograde fluorescent labeling of dental primary afferent neurons (DPANs) has been described in rats through crystalline fluorescent DiI, while in the mouse, this technique was achieved with only Fluoro-Gold, a neurotoxic fluorescent dye with membrane penetration characteristics superior to the carbocyanine dyes. We reevaluated this technique in the rat with the aim to transfer it to the mouse because comprehensive physiologic studies require access to the mouse as a model organism. Using conventional immunohistochemistry, we assessed in rats and mice the speed of axonal dye transport from the application site to the trigeminal ganglion, the numbers of stained DPANs, and the fluorescence intensity via 1) conventional crystalline DiI and 2) a novel DiI formulation with improved penetration properties and staining efficiency...
November 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/29127007/mir-3065-5p-regulates-mouse-odontoblastic-differentiation-partially-through-bone-morphogenetic-protein-receptor-type-ii
#15
Chujiao Li, Qian Zhang, Shuaitong Yu, Yuxiu Lin, Shuchen Li, Huan Liu, Zhi Chen
Illumination of the molecular mechanisms regulating odontoblastic differentiation of dental papilla cells is of great significance for proper dentinogenesis and dental pulp regeneration. In this study, we discovered that microRNA (miR)-3065-5p is up-regulated during odontoblastic differentiation. Overexpression of miR-3065-5p promoted odontoblastic differentiation in vitro. Dual luciferase report assay verified that miR-3065-5p could bind to the 3'UTR of bone morphogenetic protein receptor type II (BMPR2), which dramatically increased in the beginning of odontoblastic differentiation but decreased in the terminal differentiation stage...
November 7, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29126360/endodontic-treatment-of-canine-teeth-in-a-cynomolgus-monkey-macaca-fascicularis
#16
Caterina Facchini, Ilaria Iacopetti, Eugenio Gaudio, Alessandro Zotti, Massimo Boldrin, Fabio Fante, Franco Mutinelli, Emanuele Cozzi, Giulia Maria De Benedictis
A case of bilateral superior canine tooth pulp necrosis in an 18-year-old male Macaca fascicularis is described . A skull computed tomography scan was performed to confirm the diagnostic suspicion. A successful bilateral endodontic treatment with coronotomy was performed using rotary nickel-titanium instruments. Post-operative dental radiographs confirmed the correct positioning of the sealant. A rapid and excellent clinical recovery was obtained after each surgery. This innovative approach may contribute to improving the quality of root canal treatments provided to non-human primates...
January 1, 2017: Laboratory Animals
https://www.readbyqxmd.com/read/29123620/the-effects-of-regular-exercise-on-capsaicin-induced-pulpal-pain-and-pain-induced-changes-in-passive-avoidance-learning-and-memory-in-rats
#17
Maryam Raoof, Afshin Shakoori, Razieh Kooshki, Mehdi Abbasnejad, Sara Amanpour
Background: Pulpal pain is one of the most common and severe orofacial pain conditions with considerable adverse effects on physiological processes including learning and memory. Regular exercise is known to be effective on cognitive function as well as pain processing in the central nervous system. Here, the possible effects of regular exercise on pulpal pain response as well as pain-induced changes in learning and memory efficiency in rats were investigated. Methods: Twenty-four male Wistar rats were randomly assigned to the control, capsaicin, exercise, and exercise plus capsaicin groups...
October 2017: Korean Journal of Pain
https://www.readbyqxmd.com/read/29121452/3d-maskless-micropatterning-for-regeneration-of-highly-organized-tubular-tissues
#18
Chi Ma, Tiejun Qu, Bei Chang, Yan Jing, Jerry Q Feng, Xiaohua Liu
Micropatterning is a widely used powerful tool to create highly ordered microstructures on material surfaces. However, due to technical limitations, the integration of micropatterned microstructures into bioinspired 3D scaffolds to successfully regenerate well-organized functional tissues is not achieved. In this work, a unique maskless micropatterning technology is reported to create 3D nanofibrous matrices with highly organized tubular architecture for tissue regeneration. This micropatterning method is a laser-guided, noncontact, high-precision, flexible computer programming of machining process that can create highly ordered tubules with the density ranged from 1000 to 60 000 mm(-2) and the size varied from 300 nm to 30 µm in the bioinspired 3D matrix...
November 9, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29118330/molecular-spectrum-of-secretome-regulates-the-relative-hepatogenic-potential-of-mesenchymal-stem-cells-from-bone-marrow-and-dental-tissue
#19
Ajay Kumar, Vinod Kumar, Vidya Rattan, Vivekananda Jha, Arnab Pal, Shalmoli Bhattacharyya
Liver regeneration is a spontaneous process that occurs after liver injury, but acute liver failure is a complex and fatal disease which is difficult to treat. Cell-based therapies are promising alternative therapeutic approach for liver failure and different cell sources have been tested in this regard. We investigated the comparative hepatogenic potential of human bone marrow stem cells (BMSC) with stem cells derived from human dental pulp (DPSC), apical papilla (SCAP) and follicle (DFSC) during this study...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29117466/dental-properties-ultrastructure-and-pulp-cells-associated-with-a-novel-dspp-mutation
#20
Thantrira Porntaveetus, Thanaphum Osathanon, Nunthawan Nowwarote, Prasit Pavasant, Chalurmpon Srichomthong, Kanya Suphapeetiporn, Vorasuk Shotelersuk
OBJECTIVE: To investigate physical characteristics and behaviours of dental pulp cells of teeth isolated from a dentinogenesis imperfecta (DGI) patient with a novel DSPP mutation. SUBJECTS AND METHODS: Whole exome and Sanger sequencing were employed to identify mutations. Physical characteristics of the teeth were examined. Pulp cells' behaviors including cell proliferation, colony forming unit, osteogenic differentiation, pluripotent markers, and mesenchymal stem cell markers were investigated...
November 8, 2017: Oral Diseases
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