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Staphyloccocus aureus

Hung K Trang, R Kenneth Marcus
Polypropylene (PP) capillary-channeled polymer (C-CP) fibers loaded with recombinant Staphyloccocus aureus protein A (rSPA) were used as an affinity chromatography stationary phase for the quantitation of immunoglobulin G (IgG) in complex biological matrices. Optimization of the chromatographic method regarding mobile phase components and load/elution conditions was performed. The six-minute analysis, including a load step with 12mM phosphate at pH 7.4, an elution step with 0.025% phosphoric acid and a re-equilibration step, was employed for quantitation of IgG1 from 0...
April 22, 2017: Journal of Pharmaceutical and Biomedical Analysis
Ramon Gordon Jensen, Helle Krogh Johansen, Thomas Bjarnsholt, Steffen Robert Eickhardt-Sørensen, Preben Homøe
Dispersal of bacteria from a biofilm in the middle ear, serving as a bacterial reservoir, could explain the recurrent and chronic nature of chronic suppurative otitis media (CSOM). The objective of this study is to investigate if the same strains of bacteria could be detected in repeated episodes of otorrhea in CSOM. In a prospective case series at a primary healthcare clinic in Nuuk, Greenland, patients with more than 14 days of otorrhea were included consecutively. Samples for culturing and biofilm analysis were taken at enrollment and at any subsequent episode with otorrhea...
May 2, 2017: European Archives of Oto-rhino-laryngology
Lin Qiu, Boyao Zhang, Lang Liu, Xiaoping Wang, Chaoliang Lei, Yongjun Lin, Jing Zhao, Weihua Ma
The mitogen-activated protein kinases (MAPKs) are conserved signal transduction pathways and broadly responsible for bacterial infection from yeast to mammals, and virus, fungi, and bacteria, specifically Bacillus thuringiensis, to insects. But little is known about the MAPK pathways in antibacterial responses in Chilo suppressalis (Walker), an important lepidopteran pest of rice. In this study, we used the bacteria of Bacillus thuringiensis, Escherichia coli, and Staphyloccocus aureus to infect C. suppressalis larvae, and the responses of MAPK pathways were analyzed...
April 28, 2017: Journal of Economic Entomology
Arnaud Tomasini, Karen Moreau, Johana Chicher, Thomas Geissmann, François Vandenesch, Pascale Romby, Stefano Marzi, Isabelle Caldelari
The virulon of Staphyloccocus aureus is controlled by intricate connections between transcriptional and post-transcriptional regulators including proteins and small non-coding RNAs (sRNAs). Many of the sRNAs regulate gene expression through base-pairings with mRNAs. However, characterization of the direct sRNA targets in Gram-positive bacteria remained a difficult challenge. Here, we have applied and adapted the MS2-affinity purification approach coupled to RNA sequencing (MAPS) to determine the targetome of RsaA sRNA of S...
April 3, 2017: Nucleic Acids Research
Dinglong Yang, Qing Wang, Lizhu Chen, Yongliang Liu, Ruiwen Cao, Huifeng Wu, Fei Li, Chenglong Ji, Ming Cong, Jianmin Zhao
A phage-type lysozyme, designed as RpPLYZ, was cloned and characterized from the clam Ruditapes philippinarum. The full-length cDNA of RpPLYZ was of 699 bp with an open reading frame (ORF) of 534 bp, encoding a polypeptide of 177-amino acid with a calculated molecular mass of 19.6 kDa and an isoelectric point of 9.05. Multiple alignments and phylogenetic analysis strongly suggested that RpPLYZ was a new member of the phage-type lysozyme family. The mRNA transcript of RpPLYZ was found to be constitutively expressed in a wide range of tissues and mainly in hemocytes and mantle...
March 30, 2017: Fish & Shellfish Immunology
Robert D O'Connor, Manmilan Singh, James Chang, Sung Joon Kim, Michael VanNieuwenhze, Jacob Schaefer
We have used C{F}, N{F}, and N{P} rotational-echo double resonance NMR to determine the location and conformation of (19)F and (15)N double-labeled plusbacin A3 and of double-labeled deslipo-plusbacin A3, each bound to the cell walls of whole cells of Staphyloccocus aureus grown in media containing [1-(13)C]glycine. The (31)P is primarily in wall teichoic acid. Approximately 25% of plusbacin headgroups (the cyclic depsipeptide backbone) are in a closed conformation (N-F separation of 6 Å), while 75% are in a more open conformation (N-F separation of 12 Å)...
February 13, 2017: Journal of Physical Chemistry. B
Janina Eisenbeis, Henrik Peisker, Christian S Backes, Stephanie Bur, Sebastian Hölters, Nicolas Thewes, Markus Greiner, Christian Junker, Eva C Schwarz, Markus Hoth, Kerstin Junker, Klaus T Preissner, Karin Jacobs, Mathias Herrmann, Markus Bischoff
Staphyloccocus aureus is a major human pathogen and a common cause for superficial and deep seated wound infections. The pathogen is equipped with a large arsenal of virulence factors, which facilitate attachment to various eukaryotic cell structures and modulate the host immune response. One of these factors is the extracellular adherence protein Eap, a member of the "secretable expanded repertoire adhesive molecules" (SERAM) protein family that possesses adhesive and immune modulatory properties. The secreted protein was previously shown to impair wound healing by interfering with host defense and neovascularization...
February 2017: International Journal of Medical Microbiology: IJMM
Zeinab Jahed, Hamed Shahsavan, Mohit S Verma, Jacob L Rogowski, Brandon B Seo, Boxin Zhao, Ting Y Tsui, Frank X Gu, Mohammad R K Mofrad
Bacteria have evolved as intelligent microorganisms that can colonize and form highly structured and cooperative multicellular communities with sophisticated singular and collective behaviors. The initial stages of colony formation and intercellular communication are particularly important to understand and depend highly on the spatial organization of cells. Controlling the distribution and growth of bacterial cells at the nanoscale is, therefore, of great interest in understanding the mechanisms of cell-cell communication at the initial stages of colony formation...
January 9, 2017: ACS Nano
Ryo Kobayashi, Shinya Otomo, Yusuke Shiba, Keiichi Ebinuma, Toshiaki Sudoh
 According to a recent study and meta-analysis, trough levels of >10 μg/mL teicoplanin (TEIC) may be acceptable for the treatment of uncomplicated infection, but no method of TEIC personalized medicine has been established. Vancomycin (VCM) and TEIC are glycopeptide antibiotic agents effective against methicillin-resistance Staphyloccocus aureus. This study aimed to establish TEIC personalized medicine at a steady state calculated by VCM pharmacokinetic parameters. Bayesian forecasting and population mean methods were employed to estimate individual total VCM clearance (CL) using existing population pharmacokinetics (PPK) parameter, and the differences between the CL calculated by these two methods were defined as ΔCL...
2016: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
Abdul Sajid, Natasha Kashif, Nasira Kifayat, Shabeer Ahmad
The antibiotic residues in poultry meat can pose certain hazards to human health among them are sensitivity to antibiotics, allergic reactions, mutation in cells, imbalance of intestinal micro biota and bacterial resistance to antibiotics. The purpose of the present paper was to detect antibiotic residue in poultry meat. During the present study a total of 80 poultry kidney and liver samples were collected and tested for detection of different antibiotic residues at different pH levels Eschericha coli at pH 6, 7 and Staphyloccocus aureus at pH 8 & 9...
September 2016: Pakistan Journal of Pharmaceutical Sciences
Zhen-Qiang Shi, Yu-Ting Cai, Jie Deng, Wei-Feng Zhao, Chang-Sheng Zhao
A facile method to construct reversible thermoresponsive switching for bacteria killing and detachment was currently developed by host-guest self-assembly of β-cyclodextrin (β-CD) and adamantane (Ad). Ad-terminated poly(N-isopropylacrylamide) (Ad-PNIPAM) and Ad-terminated poly[2-(methacryloyloxy)ethyl]trimethylammonium chloride (Ad-PMT) were synthesized via atom transfer radical polymerization, and then assembled onto the surface of β-CD grafted silicon wafer (SW-CD) by simply immersing SW-CD into a mixed solution of Ad-PNIPAM and Ad-PMT, thus forming a thermoresponsive surface (SW-PNIPAM/PMT)...
September 14, 2016: ACS Applied Materials & Interfaces
J R Almeida, M Lancellotti, A M Soares, L A Calderon, D Ramírez, W González, S Marangoni, S L Da Silva
Snake venoms are rich and intriguing sources of biologically-active molecules that act on target cells, modulating a diversity of physiological functions and presenting promising pharmacological applications. Lys49 phospholipase A2 is one of the multifunctional proteins present in these complex secretions and, although catalytically inactive, has a variety of biological activities, including cytotoxic, antibacterial, inflammatory, antifungal activities. Herein, a Lys49 phospholipase A2, denominated CoaTx-II from Crotalus oreganus abyssus, was purified and structurally and pharmacologically characterized...
September 15, 2016: Toxicon: Official Journal of the International Society on Toxinology
Clémentine Samson, Aline Tamalet, Hoang Vu Thien, Jessica Taytard, Caroline Perisson, Nadia Nathan, Annick Clement, Pierre-Yves Boelle, Harriet Corvol
BACKGROUND: Low-dose azithromycin has beneficial effects on severity of the lung disease in cystic fibrosis (CF) patients for a period of 6-12 months after initiation of the treatment. Although its impact in the longer term is uncertain, this treatment is frequently used chronically. The aim of this retrospective study was to investigate the effects of low-dose azithromycin treatment on the progression of CF lung disease in patients treated for more than 12 months. METHODS: All of the CF patients followed in our pediatric center and who had been on low-dose azithromycin for more than 12 sequential months were included...
August 2016: Respiratory Medicine
Íris Neto, Joana Andrade, A S Fernandes, Catarina Pinto Reis, Jagadish K Salunke, Arri Priimagi, Nuno R Candeias, Patrícia Rijo
In this work we report the antibacterial activity of alkylaminophenols. A series of such compounds was prepared by a multicomponent Petasis-borono Mannich reaction starting from salicylaldehyde and its derivatives. The obtained compounds were tested against a large panel of microorganisms, Gram-positive and Gram-negative bacteria, and a yeast. Among the several tertiary amine derivatives tested, indoline-derived aminophenols containing a nitro group at the para-phenol position showed considerable activity against bacteria tested with minimal inhibitory concentrations as low as 1...
September 20, 2016: ChemMedChem
Vaya W Tanamai, Brandon-Luke L Seagle, Guoyang Luo
BACKGROUND: The incidence of community-acquired methicillin-resistant Staphylococcus aureus (MRSA) infection is increasing, and 0.5-4% of pregnant women are colonized. CASE: A 30-year-old pregnant woman at term presented with intractable headache 1 week after incision and drainage of a MRSA-positive axillary abscess. Imaging demonstrated a right-sided epidural abscess with midline shift and myositis of the overlying temporalis muscle. She underwent cesarean delivery followed by craniectomy of osteomyelitic bone and evacuation of the epidural abscess...
May 2016: Journal of Reproductive Medicine
Jennifer S McDanel, Eli N Perencevich, Jeremy Storm, Daniel J Diekema, Loreen Herwaldt, J Kristie Johnson, Patricia L Winokur, Marin L Schweizer
We retrospectively analyzed data for 195 respiratory infection patients who had positive Staphyloccocus aureus cultures and who were hospitalized in 2 hospitals in Iowa and Maryland, USA, during 2003-2009. Odds for death for patients who also had influenza-positive test results were >4 times higher than for those who had negative influenza test results.
July 2016: Emerging Infectious Diseases
Ying Huang, Kaimin Hui, Min Jin, Shaowu Yin, Wen Wang, Qian Ren
Calnexin (Cnx) and calreticulin (Crt), which are important chaperones in the endoplasmic reticulum (ER), participate in the folding and quality control of client proteins. Cnx and Crt identified from Chinese mitten crab (Eriocheir sinensis) are designated as EsCnx and EsCrt, respectively. EsCnx and EsCrt are expressed in the hemocyte, hepatopancrea, gill, and intestine at the mRNA and protein level. Immunofluorescence analysis indicated that EsCnx and EsCRT are located in the ER. Moreover, the mRNA and protein expression levels of EsCnx and EsCrt were altered by challenge with lipopolysaccharides (LPS), peptidoglycans (PGN), Staphyloccocus aureus, and Vibrio parahaemolyticus...
2016: Scientific Reports
Guillermo Cebrián, Pilar Mañas, Santiago Condón
In this paper the resistance of bacterial foodborne pathogens to manosonication (MS), pulsed electric fields (PEFs), high hydrostatic pressure (HHP), and UV-light (UV) is reviewed and compared. The influence of different factors on the resistance of bacterial foodborne pathogens to these technologies is also compared and discussed. Only results obtained under harmonized experimental conditions have been considered. This has allowed us to establish meaningful comparisons and draw significant conclusions. Among the six microorganisms here considered, Staphyloccocus aureus is the most resistant foodborne pathogen to MS and HHP and Listeria monocytogenes to UV...
2016: Frontiers in Microbiology
Nicholas R Saichek, Christopher R Cox, Seungki Kim, Peter B Harrington, Nicholas R Stambach, Kent J Voorhees
BACKGROUND: The Staphylococcus genus is composed of 44 species, with S. aureus being the most pathogenic. Isolates of S. aureus are generally susceptible to β-lactam antibiotics, but extensive use of this class of drugs has led to increasing emergence of resistant strains. Increased occurrence of coagulase-negative staphylococci as well as S. aureus infections, some with resistance to multiple classes of antibiotics, has driven the necessity for innovative options for treatment and infection control...
April 23, 2016: BMC Microbiology
Ying Huang, Wen Wang, Qian Ren
gC1qR is identified as the globular "head" binding protein of the C1q protein and performs an important function in innate immunity. A EsgC1qR gene was identified from the hepatopancreas of Eriocheir sinensis. EsgC1qR encodes a protein with 275 amino acids. Phylogenetic analysis showed that, together with crustaceans gC1qRs, EsgC1qR belongs to one group. EsgC1qR mRNA was detected in hemocytes, intestine, hepatopancreas, gills, eyestalk, heart, muscle, and nerve. The expression of the EsgC1qR transcript in the hepatopancreas could be regulated by lipopolysaccharides (LPS), peptidoglycans (PGN), Staphyloccocus aureus, or Vibrio parahaemolyticus...
August 2016: Developmental and Comparative Immunology
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