Oliver Hahn, Aulden G Foltz, Micaiah Atkins, Blen Kedir, Patricia Moran-Losada, Ian H Guldner, Christy Munson, Fabian Kern, Róbert Pálovics, Nannan Lu, Hui Zhang, Achint Kaur, Jacob Hull, John R Huguenard, Sebastian Grönke, Benoit Lehallier, Linda Partridge, Andreas Keller, Tony Wyss-Coray
Aging is the key risk factor for cognitive decline, yet the molecular changes underlying brain aging remain poorly understood. Here, we conducted spatiotemporal RNA sequencing of the mouse brain, profiling 1,076 samples from 15 regions across 7 ages and 2 rejuvenation interventions. Our analysis identified a brain-wide gene signature of aging in glial cells, which exhibited spatially defined changes in magnitude. By integrating spatial and single-nucleus transcriptomics, we found that glial aging was particularly accelerated in white matter compared with cortical regions, whereas specialized neuronal populations showed region-specific expression changes...
September 14, 2023: Cell