Mark Cartwright, Martin Rottman, Nathan I Shapiro, Benjamin Seiler, Patrick Lombardo, Nazita Gamini, Julie Tomolonis, Alexander L Watters, Anna Waterhouse, Dan Leslie, Dana Bolgen, Amanda Graveline, Joo H Kang, Tohid Didar, Nikolaos Dimitrakakis, David Cartwright, Michael Super, Donald E Ingber
BACKGROUND: Blood cultures, and molecular diagnostic tests that directly detect pathogen DNA in blood, fail to detect bloodstream infections in most infected patients. Thus, there is a need for a rapid test that can diagnose the presence of infection to triage patients, guide therapy, and decrease the incidence of sepsis. METHODS: An Enzyme-Linked Lectin-Sorbent Assay (ELLecSA) that uses magnetic microbeads coated with an engineered version of the human opsonin, Mannose Binding Lectin, containing the Fc immunoglobulin domain linked to its carbohydrate recognition domain (FcMBL) was developed to quantify pathogen-associated molecular patterns (PAMPs) in whole blood...
July 2016: EBioMedicine