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https://www.readbyqxmd.com/read/28339012/heavy-metal-content-and-molecular-species-identification-in-canned-tuna-insights-into-human-food-safety
#1
Anna Maria Pappalardo, Chiara Copat, Venera Ferrito, Alfina Grasso, Margherita Ferrante
Canned tuna in olive oil and in brine of the most popular brands sold in Italian markets were analyzed to verify the authentication of transformed products, with the aim to unveil commercial frauds due to the substitutions of high value species with species of low commercial value, and to assess the health risk of consumers related to cadmium (Cd), lead (Pb) and mercury (Hg) contents. Species authentication was evaluated with amplification of COI DNA barcode and confirmed the declared species. Among tested metals, Hg had the highest concentrations, followed by Cd and Pb...
March 24, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28338632/a-polynomial-subset-based-efficient-multi-party-key-management-system-for-lightweight-device-networks
#2
Zahid Mahmood, Huansheng Ning, AtaUllah Ghafoor
Wireless Sensor Networks (WSNs) consist of lightweight devices to measure sensitive data that are highly vulnerable to security attacks due to their constrained resources. In a similar manner, the internet-based lightweight devices used in the Internet of Things (IoT) are facing severe security and privacy issues because of the direct accessibility of devices due to their connection to the internet. Complex and resource-intensive security schemes are infeasible and reduce the network lifetime. In this regard, we have explored the polynomial distribution-based key establishment schemes and identified an issue that the resultant polynomial value is either storage intensive or infeasible when large values are multiplied...
March 24, 2017: Sensors
https://www.readbyqxmd.com/read/28338620/a-secure-and-privacy-preserving-navigation-scheme-using-spatial-crowdsourcing-in-fog-based-vanets
#3
Lingling Wang, Guozhu Liu, Lijun Sun
Fog-based VANETs (Vehicular ad hoc networks) is a new paradigm of vehicular ad hoc networks with the advantages of both vehicular cloud and fog computing. Real-time navigation schemes based on fog-based VANETs can promote the scheme performance efficiently. In this paper, we propose a secure and privacy-preserving navigation scheme by using vehicular spatial crowdsourcing based on fog-based VANETs. Fog nodes are used to generate and release the crowdsourcing tasks, and cooperatively find the optimal route according to the real-time traffic information collected by vehicles in their coverage areas...
March 24, 2017: Sensors
https://www.readbyqxmd.com/read/28337740/proteome-comparison-for-discrimination-between-honeydew-and-floral-honeys-from-botanical-specie-mimosa-scabrella-bentham-by-principal-component-analysis
#4
Mônia Stremel Azevedo, Pedro Alexandre Valentim-Neto, Siluana Katia Tischer Seraglio, Cynthia Fernandes Pinto da Luz, Ana Carolina Maisonnave Arisi, Ana Carolina Oliveira Costa
BACKGROUND: Due to increasing valuation and appreciation of honeydew honey in many European countries and also to existing contamination among different kinds of honeys, the authentication is an important aspect of quality control and its regard with the origin guarantee in terms of source, honeydew or floral, needs to be determined. Furthermore, belonging to the minority composition of the honey, beyond of the importance of its physiological effects, proteins can differ according to these sources...
March 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28335572/efficient-and-security-enhanced-anonymous-authentication-with-key-agreement-scheme-in-wireless-sensor-networks
#5
Jaewook Jung, Jongho Moon, Donghoon Lee, Dongho Won
At present, users can utilize an authenticated key agreement protocol in a Wireless Sensor Network (WSN) to securely obtain desired information, and numerous studies have investigated authentication techniques to construct efficient, robust WSNs. Chang et al. recently presented an authenticated key agreement mechanism for WSNs and claimed that their authentication mechanism can both prevent various types of attacks, as well as preserve security properties. However, we have discovered that Chang et al's method possesses some security weaknesses...
March 21, 2017: Sensors
https://www.readbyqxmd.com/read/28335453/a-spatially-offset-raman-spectroscopy-method-for-non-destructive-detection-of-gelatin-encapsulated-powders
#6
Kuanglin Chao, Sagar Dhakal, Jianwei Qin, Yankun Peng, Walter F Schmidt, Moon S Kim, Diane E Chan
Non-destructive subsurface detection of encapsulated, coated, or seal-packaged foods and pharmaceuticals can help prevent distribution and consumption of counterfeit or hazardous products. This study used a Spatially Offset Raman Spectroscopy (SORS) method to detect and identify urea, ibuprofen, and acetaminophen powders contained within one or more (up to eight) layers of gelatin capsules to demonstrate subsurface chemical detection and identification. A 785-nm point-scan Raman spectroscopy system was used to acquire spatially offset Raman spectra for an offset range of 0 to 10 mm from the surfaces of 24 encapsulated samples, using a step size of 0...
March 18, 2017: Sensors
https://www.readbyqxmd.com/read/28334169/characterization-of-saccharomyces-uvarum-beijerinck-1898-and-related-hybrids-assessment-of-molecular-markers-that-predict-the-parent-and-hybrid-genomes-and-a-proposal-to-name-yeast-hybrids
#7
Huu-Vang Nguyen, Teun Boekhout
The use of the nuclear-DNA re-association technique has led taxonomists to consider Saccharomyces uvarum a synonym of S. bayanus. The latter, however, is not a species but a hybrid harbouring S. eubayanus (Seu) and S. uvarum (Su) sub-genomes with a minor DNA contribution from S. cerevisiae (Sc). To recognize genetically pure lines of S. uvarum and putative interspecies hybrids among so-called S. bayanus strains present in public culture collections, we propose the use of four markers that were defined from the S...
March 3, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28332826/packaged-and-free-stmv-rna-genomes-adopt-distinct-conformational-states
#8
Bridget C Larman, Elizabeth A Dethoff, Kevin M Weeks
The RNA genomes of viruses likely undergo multiple functionally important conformational changes during their replication cycles, changes that are poorly understood at present. We used two complementary in-solution RNA structure probing strategies (SHAPE-MaP and RING-MaP) to examine the structure of the RNA genome of satellite tobacco mosaic virus inside authentic virions and in a capsid-free state. Both RNA states feature similar three-domain architectures in which each major replicative function - translation, capsid coding, and genome synthesis - fall into distinct domains...
March 23, 2017: Biochemistry
https://www.readbyqxmd.com/read/28331098/recombinant-modified-vaccinia-virus-ankara-generating-ebola-virus-like-particles
#9
Marc Schweneker, Andrea S Laimbacher, Gert Zimmer, Susanne Wagner, Elisabeth M Schraner, Michael Wolferstätter, Marieken Klingenberg, Ulrike Dirmeier, Robin Steigerwald, Henning Lauterbach, Hubertus Hochrein, Paul Chaplin, Mark Suter, Jürgen Hausmann
There are currently no approved therapeutics or vaccines to treat or protect against the severe hemorrhagic fever and death caused by Ebola virus (EBOV). Ebola virus-like particles (EBOV-VLPs) consisting of the matrix protein VP40, the glycoprotein (GP) and the nucleoprotein (NP) are highly immunogenic and protective in non-human primates against Ebola virus disease (EVD). We have constructed a modified vaccinia virus Ankara-Bavarian Nordic(®) (MVA-BN(®)) recombinant co-expressing VP40 and glycoprotein (GP) of EBOV Mayinga and the nucleoprotein (NP) of Taï Forest virus (TAFV) (MVA-BN-EBOV-VLP) to launch non-infectious EBOV-VLPs as a second vaccine modality in the MVA-BN-EBOV-VLP-vaccinated organism...
March 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28330747/m-protein-is-sufficient-for-assembly-and-release-of-peste-des-petits-ruminants-virus-like-particles
#10
Qiuxia Wang, Changbo Ou, Yongxi Dou, Lei Chen, Xuelian Meng, Xingyou Liu, Yan Yu, Jinqing Jiang, Jinyou Ma, Zhidong Zhang, Jianhe Hu, Xuepeng Cai
Peste des petits ruminants virus (PPRV), belonging to paramyxoviruses, has six structure proteins (such as matrix protein (M), nucleocapsid proteins (N), fusion protein (F) and hemagglutinin protein (H)) and could cause high morbidity and mortality in sheep and goats. Although a vaccine strain of PPRV has been rescued and co-expression of M and N could yield PPRV-like particles, the roles of structure proteins in virion assembly and release have not been investigated in detail. In this study, plasmids carrying PPRV cDNA sequences encoding the N, M, H, and F proteins were expressed in Vero cells...
March 18, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28328394/proposing-telecardiology-services-on-cloud-for-different-medical-institutions-a-model-of-reference
#11
Isabel De la Torre-Díez, Begoña Garcia-Zapirain, Miguel López-Coronado, Joel J P C Rodrigues
INTRODUCTION: For a cloud-based telecardiology solution to be established in any scenario, it is necessary to ensure optimum levels of security, as patient's data will not be in the same place from where access is gained. The main objective of this article is to present a secure, cloud-based solution for a telecardiology service in different scenarios: a hospital, a health center in a city, and a group of health centers in a rural area. METHODS: iCanCloud software is used to simulate the scenarios...
March 1, 2017: Telemedicine Journal and E-health: the Official Journal of the American Telemedicine Association
https://www.readbyqxmd.com/read/28327936/guidock-vnc-using-a-graphical-desktop-sharing-system-to-provide-a-browser-based-interface-for-containerized-software
#12
Varun Mittal, Ling-Hong Hung, Jayant Keswani, Daniel Kristiyanto, Sung Bong Lee, Ka Yee Yeung
Background: Software container technology such as Docker can be used to package and distribute bioinformatics workflows consisting of multiple software implementations and dependencies. However, Docker is a command line based tool and many bioinformatics pipelines consist of components that require a graphical user interface. Findings: We present a container tool called GUIdock-VNC that uses a graphical desktop sharing system to provide a browser-based interface for containerized software...
February 24, 2017: GigaScience
https://www.readbyqxmd.com/read/28327479/finger-printing-of-counterfeit-bevacizumab-and-identifying-it-before-clinical-use
#13
Thirumurthy Velpandian, Madhu Nath, Moksha Laxmi, Nabanita Halder
BACKGROUND: Bevacizumab is widely used for ophthalmic purposes. Recently, counterfeit bevacizumab has become a matter of concern. We analysed samples of suspected counterfeit formulations of bevacizumab and assessed the possibility of using simple tests in the clinic by ophthalmologists to prevent the use of counterfeit preparations in patients. METHODS: We did a protein analysis using Bradford assay and SDS-PAGE to confirm the presence of bevacizumab in 16 samples - 6 suspected and 10 others...
November 2016: National Medical Journal of India
https://www.readbyqxmd.com/read/28326615/using-passive-sampling-and-zebrafish-to-identify-developmental-toxicants-in-complex-mixtures
#14
Alan J Bergmann, Robert L Tanguay, Kim A Anderson
Using effects-directed analysis (EDA), we investigate associations previously observed between PAHs and embryotoxicity in field-deployed low-density polyethylene (LDPE). We conducted EDA using a zebrafish embryo assay and iterative fractionation of extracts of LDPE that were deployed in Portland Harbor Superfund Megasite, Oregon. Whole extracts induced toxicity including mortality, edema, and notochord distortion at EC20s of approximately 100, 100, and 10 mg LDPE/mL, respectively. Through fractionation, we determined that PAHs at concentrations similar to previous research did not contribute markedly to toxicity...
March 22, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28325170/rapid-discrimination-between-buffalo-and-cow-milk-and-detection-of-adulteration-of-buffalo-milk-with-cow-milk-using-synchronous-fluorescence-spectroscopy-in-combination-with-multivariate-methods
#15
Serap Durakli Velioglu, Elif Ercioglu, Ismail Hakki Boyaci
This research paper describes the potential of synchronous fluorescence (SF) spectroscopy for authentication of buffalo milk, a favourable raw material in the production of some premium dairy products. Buffalo milk is subjected to fraudulent activities like many other high priced foodstuffs. The current methods widely used for the detection of adulteration of buffalo milk have various disadvantages making them unattractive for routine analysis. Thus, the aim of the present study was to assess the potential of SF spectroscopy in combination with multivariate methods for rapid discrimination between buffalo and cow milk and detection of the adulteration of buffalo milk with cow milk...
March 22, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28323541/3d-technology-of-sony-bloggie-has-no-advantage-in-decision-making-of-tennis-serve-direction-a-randomized-placebo-controlled-study
#16
Sicong Liu, Jason Ritchie, Camilo Sáenz-Moncaleano, Savanna K Ward, Cody Paulsen, Tyler Klein, Oscar Gutierrez, Gershon Tenenbaum
This study aimed at exploring whether 3D technology enhances tennis decision-making under the conceptual framework of human performance model. A 3 (skill-level: varsity, club, recreational) × 3 (experimental condition: placebo, weak 3D [W3D], strong 3D [S3D]) between-participant design was used. Allocated to experimental conditions by a skill-level stratified randomization, 105 tennis players judged tennis serve direction from video scenarios and rated their perceptions of enjoyment, flow, and presence during task performance...
March 21, 2017: European Journal of Sport Science
https://www.readbyqxmd.com/read/28323244/itraq-based-differential-proteomic-analysis-in-mongolian-gerbil-brains-chronically-infected-with-toxoplasma-gondii
#17
Lin Lv, Yapei Wang, Weili Feng, Jorge A Hernandez, Wanyi Huang, Yuxiang Zheng, Xue Zhou, Shumei Lv, Yajun Chen, Zi-Guo Yuan
The aim of our study was to detect differentially regulated proteins and specific signaling pathways in Mongolian gerbil brains during chronic Toxoplasma gondii (T.gondii) PRU strain infection. We use a iTRAQ-based strategy to detecte 4935 proteins, out of which 110 proteins were differentially expressed (>/=2.0-fold, p value <0.05) when the brain of gerbils infected with T.gondii was compared to control brain tissues. We confirmed the authenticity and the accuracy of iTRAQ results through quantitative real-time PCR and western blot (WB), which was consistent with mass spectrometry analysis...
March 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28321933/exploring-the-cause-of-aggregation-and-reduced-zn-binding-affinity-by-g85r-mutation-in-sod1-rendering-amyotrophic-lateral-sclerosis
#18
E Srinivasan, R Rajasekaran
Amyotrophic lateral sclerosis (ALS), a lethal neurodegenerative disorder is characterized by the degeneration of upper and lower motor neuron. ALS occurs due to various notably prominent missense mutations, in gene encoding Cu-Zn superoxide dismutase (SOD1) thereby leading to aggregation, dysfunction and reduced Zn binding affinity. In this study, one such mutation, G85R was explored in comparison with wild type SOD1, using discrete molecular dynamics (DMD). Accordingly, the conformational changes were significantly observed in mutant SOD1, through various geometrical parameters, which substantiated the difference in conformational deviation, flexibility and compactness, thus stipulating a root cause for aggregation...
March 21, 2017: Proteins
https://www.readbyqxmd.com/read/28320678/reinforced-robust-principal-component-pursuit
#19
Pratik Prabhanjan Brahma, Yiyuan She, Shijie Li, Jiade Li, Dapeng Wu
High-dimensional data present in the real world is often corrupted by noise and gross outliers. Principal component analysis (PCA) fails to learn the true low-dimensional subspace in such cases. This is the reason why robust versions of PCA, which put a penalty on arbitrarily large outlying entries, are preferred to perform dimension reduction. In this paper, we argue that it is necessary to study the presence of outliers not only in the observed data matrix but also in the orthogonal complement subspace of the authentic principal subspace...
March 14, 2017: IEEE Transactions on Neural Networks and Learning Systems
https://www.readbyqxmd.com/read/28320664/blind-forensics-of-successive-geometric-transformations-in-digital-images-using-spectral-method-theory-and-applications
#20
Jiangqun Ni, Chenglong Chen, Zhaoyi Shen, Yun-Qing Shi
Geometric transformations, such as resizing and rotation, are almost always needed when two or more images are spliced together to create convincing image forgeries. In recent years, researchers have developed many digital forensic techniques to identify these operations. Most previous works in this area focus on the analysis of images that have undergone single geometric transformations, e.g., resizing or rotation. In several recent works, researchers have addressed yet another practical and realistic situation: successive geometric transformations, e...
March 15, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
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