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https://www.readbyqxmd.com/read/29793194/simultaneous-determination-of-18-psychoactive-agents-and-6-metabolites-in-plasma-using-lc-ms-ms-and-application-to-actual-plasma-samples-from-conscription-candidates
#1
Dongeun Park, Hyeyoung Choi, Moonhee Jang, Hyejin Chang, Sanghee Woo, Wonkyung Yang
In Korea, an increasing number of people attempt to evade military conscription by posing as mental health patients. To verify the authenticity of mental illness, there is a need to detect wide range of psychoactive agents in biological specimens of conscription candidates. In this study, we developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of 18 psychoactive agents and 6 metabolites in human plasma. The method was characterized by the use of a simple, fast and cheap protein precipitation as sample preparation, a rapid run time (11min) and a low volume of plasma sample (200μL)...
April 22, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29791907/toll-like-receptor-3-signaling-contributes-to-regional-neutrophil-recruitment-in-cultured-human-glomerular-endothelial-cells
#2
Qiang Liu, Tadaatsu Imaizumi, Shogo Kawaguchi, Tomomi Aizawa, Tomoh Matsumiya, Shojiro Watanabe, Koji Tsugawa, Hidemi Yoshida, Kazushi Tsuruga, Kensuke Joh, Hiroshi Kijima, Hiroshi Tanaka
BACKGROUND: Given the importance of neutrophil recruitment in the pathogenesis of glomerulonephritis (GN), the representative neutrophil chemoattractant C-X-C motif chemokine 1 (CXCL1)/GROα and the adhesion molecule E-selectin in glomerular endothelial cells (GECs) play a pivotal role in the development of GN. Endothelial Toll-like receptor 3 (TLR3) is thought to be involved in the inflammatory response via innate immunity. However, the role of endothelial TLR3 signaling in the expression of neutrophil chemoattractants and adhesion molecules remains to be elucidated...
May 23, 2018: Nephron
https://www.readbyqxmd.com/read/29791890/a-robust-and-non-invertible-fingerprint-template-for-fingerprint-matching-system
#3
Amit Kumar Trivedi, Dalton Meitei Thounaojam, Shyamosree Pal
Fingerprint Recognition System is widely deployed in variety of application domain, ranging from forensic to mobile phones. Its widespread deployment in various applications were person authentication are required, has caused concern that a leaked fingerprint template may be used to reconstruct the original fingerprint and the reconstructed fingerprint can be used to circumvent all the applications the person is enrolled. In this paper, a non-invertible fingerprint template that stores only the relative geometric information about the minutiae points is proposed...
May 20, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29791777/counting-on-death-quantitative-aspects-of-bcl-2-family-regulation
#4
REVIEW
Annika Hantusch, Markus Rehm, Thomas Brunner
The Bcl-2 protein family members critically regulate mitochondrial outer membrane permeabilization (MOMP), a point-of-no-return in the intrinsic and extrinsic apoptosis pathways. The common view on qualitative interaction and activation patterns of the three subclasses, the BH3-only proteins, prosurvivals and effectors, is static and currently being revolutionized by an emerging understanding of the complex dynamic equilibria that govern cellular fate. Recent experimental evidence on protein associations with the mitochondrial outer membrane, retrotranslocation to the cytosol and differential binding affinities in aqueous and membranous environments instigate the development of a revised model of Bcl-2 family interplay...
May 23, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29791434/food-fraud-and-the-perceived-integrity-of-european-food-imports-into-china
#5
H Kendall, P Naughton, S Kuznesof, M Raley, M Dean, B Clark, H Stolz, R Home, M Y Chan, Q Zhong, P Brereton, L J Frewer
BACKGROUND/AIMS: Persistent incidents of food fraud in China have resulted in low levels of consumer trust in the authenticity and safety of food that is domestically produced. We examined the relationship between the concerns of Chinese consumers regarding food fraud, and the role that demonstrating authenticity may play in relieving those concerns. METHODS: A two-stage mixed method design research design was adopted. First, qualitative research (focus groups n = 7) was conducted in three Chinese cities, Beijing, Guangzhou and Chengdu to explore concerns held by Chinese consumers in relation to food fraud...
2018: PloS One
https://www.readbyqxmd.com/read/29790957/cultivating-collaborations-site-specific-design-for-embodied-science-learning
#6
Katherine Gill, Jocelyn Glazier, Betsy Towns
Immersion in well-designed outdoor environments can foster the habits of mind that enable critical and authentic scientific questions to take root in students' minds. Here we share two design cases in which careful, collaborative, and intentional design of outdoor learning environments for informal inquiry provide people of all ages with embodied opportunities to learn about the natural world, developing the capacity for understanding ecology and the ability to empathize, problem-solve and reflect. Embodied learning, as facilitated by and in well-designed outdoor learning environments, leads students to develop new ways of seeing, new scientific questions, new ways to connect with ideas, with others and new ways of thinking about the natural world...
May 21, 2018: Integrative and Comparative Biology
https://www.readbyqxmd.com/read/29790652/preparation-for-making-clinical-referrals
#7
Shaun P Qureshi, Katharine Rankin, Neill Storrar, Michael Freeman
BACKGROUND: The application of prior learning within medical curricula to real patient care is challenging. Clinical assistantships support UK medical students making the transition to postgraduate practice as doctors. This paper describes a method of teaching clinical referrals: the process of clinicians contacting colleagues for advice or services. The skills required are key to medical practice, and students should be supported to develop them in order to optimally benefit from their assistantships and prepare for practice...
May 23, 2018: Clinical Teacher
https://www.readbyqxmd.com/read/29790393/assistive-technology-policy-a-position-paper-from-the-first-global-research-innovation-and-education-on-assistive-technology-great-summit
#8
Malcolm MacLachlan, David Banes, Diane Bell, Johan Borg, Brian Donnelly, Michael Fembek, Ritu Ghosh, Rosie Gowran, Emma Hannay, Diana Hiscock, Evert-Jan Hoogerwerf, Tracey Howe, Friedbert Kohler, Natasha Layton, Siobhán Long, Hasheem Mannan, Gubela Mji, Thomas Odera Ongolo, Katherine Perry, Cecilia Pettersson, Jessica Power, Vinicius Delgado Ramos, Lenka Slepičková, Emma Smith, Kiu Tay-Teo, Priscille Geiser, Hilary Hooks
Increased awareness, interest and use of assistive technology (AT) presents substantial opportunities for many citizens to become, or continue being, meaningful participants in society. However, there is a significant shortfall between the need for and provision of AT, and this is patterned by a range of social, demographic and structural factors. To seize the opportunity that assistive technology offers, regional, national and sub-national assistive technology policies are urgently required. This paper was developed for and through discussion at the Global Research, Innovation and Education on Assistive Technology (GREAT) Summit; organized under the auspices of the World Health Organization's Global Collaboration on Assistive Technology (GATE) program...
May 23, 2018: Disability and Rehabilitation. Assistive Technology
https://www.readbyqxmd.com/read/29789688/rapid-authentication-of-ginkgo-biloba-herbal-products-using-the-recombinase-polymerase-amplification-assay
#9
Yang Liu, Xiao-Yue Wang, Xue-Min Wei, Zi-Tong Gao, Jian-Ping Han
Species adulteration in herbal products (HPs) exposes consumers to health risks. Chemical and morphological methods have their own deficiencies when dealing with the detection of species containing the same active compounds in HPs. In this study, we developed a rapid identification method using the recombinase polymerase amplification (RPA) assay to detect two species, Ginkgo biloba and Sophora japonica (as adulteration), in Ginkgo biloba HPs. Among 36 Ginkgo biloba HP samples, 34 were found to have Ginkgo biloba sequences, and 9 were found to have Sophora japonica sequences...
May 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29789579/endogenous-authentic-oct4a-proteins-directly-regulate-fos-ap-1-transcription-in-somatic-cancer-cells
#10
Yanwen Zhou, Xinyu Chen, Bo Kang, Shiqi She, Xiaobing Zhang, Cheng Chen, Wenxin Li, Wenjie Chen, Songsong Dan, Xiaoyun Pan, Xiaoli Liu, Jianqin He, Qingwei Zhao, Chenggang Zhu, Ling Peng, Haoyi Wang, Hangping Yao, Hongcui Cao, Lanjuan Li, Meenhard Herlyn, Ying-Jie Wang
OCT4A is well established as a master transcription factor for pluripotent stem cell (PSC) self-renewal and a pioneer factor for initiating somatic cell reprogramming, yet its presence and functionality in somatic cancer cells remain controversial and obscure. By combining the CRISPR-Cas9-based gene editing with highly specific PCR assays, highly sensitive immunoassays, and mass spectrometry, we provide unequivocal evidence here that full-length authentic OCT4A transcripts and proteins were both present in somatic cancer cells, and OCT4A proteins were heterogeneously expressed in the whole cell population and when expressed, they are predominantly localized in cell nucleus...
May 22, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29789501/differentiation-between-organic-and-non-organic-apples-using-diffraction-grating-and-image-processing-a-cost-effective-approach
#11
Nanfeng Jiang, Weiran Song, Hui Wang, Gongde Guo, Yuanyuan Liu
As the expectation for higher quality of life increases, consumers have higher demands for quality food. Food authentication is the technical means of ensuring food is what it says it is. A popular approach to food authentication is based on spectroscopy, which has been widely used for identifying and quantifying the chemical components of an object. This approach is non-destructive and effective but expensive. This paper presents a computer vision-based sensor system for food authentication, i.e., differentiating organic from non-organic apples...
May 23, 2018: Sensors
https://www.readbyqxmd.com/read/29789475/an-identity-based-anti-quantum-privacy-preserving-blind-authentication-in-wireless-sensor-networks
#12
Hongfei Zhu, Yu-An Tan, Liehuang Zhu, Xianmin Wang, Quanxin Zhang, Yuanzhang Li
With the development of wireless sensor networks, IoT devices are crucial for the Smart City; these devices change people's lives such as e-payment and e-voting systems. However, in these two systems, the state-of-art authentication protocols based on traditional number theory cannot defeat a quantum computer attack. In order to protect user privacy and guarantee trustworthy of big data, we propose a new identity-based blind signature scheme based on number theorem research unit lattice, this scheme mainly uses a rejection sampling theorem instead of constructing a trapdoor...
May 22, 2018: Sensors
https://www.readbyqxmd.com/read/29787885/exposing-splicing-forgery-based-on-color-temperature-estimation
#13
Peng Sun, Yubo Lang, Shu Fan, Zhe Shen, Lei Liu, Daguo Shan, Silong Peng
Splicing is one of the most common tampering techniques for image manipulation in many forensic cases. Normally color shift in images due to color temperature of illumination can be seen as intrinsic features relative to imaging process. In splicing forgeries, copied area and pasted target image come from different imaging process, and are likely to have different color shift. In this paper, a novel automated authentication method is presented to expose splicing manipulation and locate manipulated areas by discriminating the inconsistencies of color shift in an image...
May 1, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29787584/method-overtness-forensic-autopsy-and-the-evidentiary-suicide-note-a-multilevel-national-violent-death-reporting-system-analysis
#14
Ian R H Rockett, Eric D Caine, Steven Stack, Hilary S Connery, Kurt B Nolte, Christa L Lilly, Ted R Miller, Lewis S Nelson, Sandra L Putnam, Paul S Nestadt, Haomiao Jia
OBJECTIVE: Higher prevalence of suicide notes could signify more conservatism in accounting and greater proneness to undercounting of suicide by method. We tested two hypotheses: (1) an evidentiary suicide note is more likely to accompany suicides by drug-intoxication and by other poisoning, as less violent and less forensically overt methods, than suicides by firearm and hanging/suffocation; and (2) performance of a forensic autopsy attenuates any observed association between overtness of method and the reported presence of a note...
2018: PloS One
https://www.readbyqxmd.com/read/29787296/short-tandem-repeat-str-profiles-of-commonly-used-human-ocular-surface-cell-lines
#15
Alison M McDermott, Hasna Baidouri, Ashley M Woodward, Wendy R Kam, Yang Liu, Xiaomin Chen, Jillian F Ziemanski, Kerry Vistisen, Linda D Hazlett, Kelly K Nichols, Pablo Argüeso, David A Sullivan
Purpose To establish the short tandem repeat (STR) profiles of several human cell lines commonly used in ocular surface research. Materials and Methods Independently DNA was extracted from multiple passages of three human corneal epithelial cell lines, two human conjunctival epithelial cell lines and one meibomian gland cell line, from different laboratories actively involved in ocular surface research. The samples were then subjected to STR analysis on a fee-for-service basis in an academic setting and the data compared against that in available databases...
May 22, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29786662/study-on-the-discrimination-between-citri-reticulatae-pericarpium-varieties-based-on-hs-spme-gc-ms-combined-with-multivariate-statistical-analyses
#16
Yuying Zheng, Xuan Zeng, Wei Peng, Zhong Wu, Weiwei Su
Citri reticulatae pericarpium (CRP), the dried pericarps of Citrus reticulata Blanco and its cultivars, has been widely used in drugs and foods in China for centuries. In this study, an accurate and feasible analytical method based on HS-SPME-GC-MS coupled with multivariate statistical analyses was developed to comprehensively compare volatile compounds of pericarps derived from Citrus reticulata "Chachi" ("Guangchenpi" in Chinese, GCP) and other cultivars of Citrus reticulata Blanco ("Chenpi" in Chinese, CP)...
May 22, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29785908/rapid-detection-of-adulteration-of-milks-from-different-species-using-fourier-transform-infrared-spectroscopy-ftir
#17
Olgun Cirak, N Cihat Icyer, M Zeki Durak
The aim of the studies reported in the Research Communication was to develop a rapid spectroscopic technique as an alternative method for the classification and discrimination of milk sources by Fourier transform infrared spectroscopy (FTIR). Cow, sheep and water buffalo milk samples were collected from various local milk producers in Istanbul, Turkey. In addition, various brands of packaged milk were purchased locally. Spectrums were obtained according to milk species origin and binary mixtures prepared in increments of 10% (10, 20, 30, 40, 50, 60, 70, 80 and 90%) for each sample analysed in FTIR spectroscopy...
May 2018: Journal of Dairy Research
https://www.readbyqxmd.com/read/29784419/development-of-a-method-for-the-elemental-analysis-of-milk-powders-using-laser-ablation-inductively-coupled-plasma-mass-spectrometry-la-icp-ms-and-its-potential-use-in-geographic-sourcing
#18
Tricia Hoffman, Radojko Jaćimović, Lian Jie Bay, Julieta Griboff, Marta Jagodic, Magdalena Monferrán, Nives Ogrinc, Ivan Podkolzin, Daniel Wunderlin, Jose Almirall
Milk has been reported as one of the most adulterated foodstuffs in the developed and developing world. One way to detect adulteration is to determine whether the country of origin on the label could be the actual country of origin. Such profiling may be accomplished through the use of elemental analysis techniques, however this is a preliminary study and this goal is not yet met. In this study, a laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) method was developed for the analysis of solid milk powder and compared to k0 -instrumental neutron activation analysis (k0 -INAA) for a reference milk material (IAEA-153) as well as several milk samples from different countries...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29781967/qualitative-research-in-correctional-settings-researcher-bias-western-ideological-influences-and-social-justice
#19
Morgan Wadams, Tanya Park
Within correctional settings, justice, health, and academic systems overlap for forensic nurse researchers. Within an environment that stresses social control, a researcher's implicit views, perspectives, and biases can lead to altering the authentic (re)presentation of a participant's experience. Researcher bias may be influenced by predominately western ideologies and societal discourses. Qualitative methods to mitigate and raise awareness around researcher biases include bracketing, unstructured interviews, diverse peer review, thinking inductively, investigator responsiveness, and critical reflexivity...
April 2018: Journal of Forensic Nursing
https://www.readbyqxmd.com/read/29781699/conceptualisation-of-african-primal-health-care-within-mental-health-care
#20
Neo E Nare, Abel J Pienaar, Ditaba D Mphuthi
BACKGROUND:  It is believed by western education systems that the first contact should be with the nurse in primary health care. However, it is not the case. Therefore, the researcher attempts to correct this misconception by conceptualising the correct beginning of health seeking behaviour in an indigenous African community, namely African Primal Health Care (APHC). 'Primal' was coined during a colloquium by Dr Mbulawa and Seboka team members; however no formal conceptualisation took place, only operational definition...
March 22, 2018: Curationis
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