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https://www.readbyqxmd.com/read/28107433/investigation-of-cross-contamination-and-misidentification-of-278-widely-used-tumor-cell-lines
#1
Yaqing Huang, Yuehong Liu, Congyi Zheng, Chao Shen
In recent years, biological research involving human cell lines has been rapidly developing in China. However, some of the cell lines are not authenticated before use. Therefore, misidentified and/or cross-contaminated cell lines are unfortunately commonplace. In this study, we present a comprehensive investigation of cross-contamination and misidentification for a panel of 278 cell lines from 28 institutes in China by using short tandem repeat profiling method. By comparing the DNA profiles with the cell bank databases of ATCC and DSMZ, a total of 46...
2017: PloS One
https://www.readbyqxmd.com/read/28106808/anti-fatigue-effects-of-the-unique-polysaccharide-marker-of-dendrobium-officinale-on-balb-c-mice
#2
Wei Wei, Zhi-Peng Li, Tong Zhu, Hau-Yee Fung, Tin-Long Wong, Xin Wen, Dik-Lung Ma, Chung-Hang Leung, Quan-Bin Han
Dendrobium officinale extract shows potent anti-fatigue effects; however, the active substance responsible for these effects remains undetermined. A glucomannan with a huge molecular size of 730 kDa, called DOP, was identified as the unique authentication marker of this expensive herb. DOP exhibited immunomodulating effects on macrophages and lymphocytes in our previous study. Clinical reports also showed that people with fatigue syndrome have a disturbed immune system. Because DOP is the unique and dominant component of D...
January 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28106247/validation-of-the-nccn-ipi-for-diffuse-large-b-cell-lymphoma-dlbcl-the-addition-of-%C3%AE-2-microglobulin-yields-a-more-accurate-geltamo-ipi
#3
Carlos Montalbán, Antonio Díaz-López, Ivan Dlouhy, Jordina Rovira, Armando Lopez-Guillermo, Sara Alonso, Alejandro Martín, Juan M Sancho, Olga García, Jose M Sánchez, Mario Rodríguez, Silvana Novelli, Antonio Salar, Antonio Gutiérrez, Maria J Rodríguez-Salazar, Mariana Bastos, Juan F Domínguez, Rubén Fernández, Sonia Gonzalez de Villambrosia, José A Queizan, Raul Córdoba, Raquel de Oña, Andrés López-Hernandez, Julian M Freue, Heidys Garrote, Lourdes López, Ana M Martin-Moreno, Jose Rodriguez, Víctor Abraira, Juan F García
The study included 1848 diffuse large B-cell lymphoma (DLBCL)patients treated with chemotherapy/rituximab. The aims were to validate the National Comprehensive Cancer Network International Prognostic Index (NCCN-IPI) and explore the effect of adding high Beta-2 microglobulin (β2M), primary extranodal presentation and intense treatment to the NCCN-IPI variables in order to develop an improved index. Comparing survival curves, NCCN-IPI discriminated better than IPI, separating four risk groups with 5-year overall survival rates of 93%, 83%, 67% and 49%, but failing to identify a true high-risk population...
January 20, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28103977/-differential-textual-research-and-analysis-on-the-evolution-of-fritillaria-species-in-successive-dynasties
#4
L Qiu
Rhizoma bolbostemmae was the earliest applied fritillaria species during the period of pre-Qin to Han Dynasties in China. In the period of Wei-Jin, the fritillaria species presented diversities, with Zhejiang-fritillary also appeared for the first time. During the period of the Tang Dynasty to late Ming Dynasty, the fritillaria species kept expanding, Fritillaria hupehensis and Zhejiang-fritillary were deemed authentic species all the time. To the end of the Ming Dynasty, Fritillaria hupehensis withdrew from the stage of history gradually, Fritillaria cirrhosa came into being and then Fritillaria cirrhosa and Zhejiang-fritillary both appeared and became two important species of medicinal fritillarias after late Ming dynasty until today...
November 28, 2016: Zhonghua Yi Shi za Zhi, Chinese Journal of Medical History
https://www.readbyqxmd.com/read/28103568/on-the-exploration-of-information-from-the-dtw-cost-matrix-for-online-signature-verification
#5
Abhishek Sharma, Suresh Sundaram
This paper explores the utility of information derived from the dynamic time warping (DTW) cost matrix for the problem of online signature verification. The prior works in literature primarily utilize only the DTW scores to authenticate a test signature. To the best of our knowledge, the characteristics of the warping path (used for the alignment) in the cost matrix is hardly exploited for verification of online signatures. Accordingly, we devise a score that utilizes the information from the cost matrix and warping path alignments...
January 17, 2017: IEEE Transactions on Cybernetics
https://www.readbyqxmd.com/read/28103421/structure-of-the-arabidopsis-thaliana-nadph-cytochrome-p450-reductase-2-atr2-provides-insight-into-its-function
#6
Guoqi Niu, Shun Zhao, Lei Wang, Wei Dong, Lin Liu, Yikun He
Members of the cytochrome P450 family catalyze a variety of monooxygenase reactions, and for the eukaryotic membrane-bound members, NADPH is typically used as the reducing agent. The flavoprotein NADPH-cytochrome P450 reductase (CPR) enables electron transfer from NADPH to cytochrome P450 via its flavin cofactors. ATR2 is one of the two authentic CPR genes in the genome of the model plant Arabidopsis thaliana, and its product has been physiologically and kinetically characterized. Here, we report the 2.3-Å structure of ATR2 and find that the position of the two flavin cofactors differs from that of other known CPR structures...
January 19, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28101269/metagenomic-approaches-to-identify-novel-organisms-from-the-soil-environment-in-a-classroom-setting
#7
Sadia J Rahman, Trevor C Charles, Parjit Kaur
Molecular Microbial Metagenomics is a research-based undergraduate course developed at Georgia State University. This semester-long course provides hands-on research experience in the area of microbial diversity and introduces molecular approaches to study diversity. Students are part of an ongoing research project that uses metagenomic approaches to isolate clones containing 16S ribosomal ribonucleic acid (rRNA) genes from a soil metagenomic library. These approaches not only provide a measure of microbial diversity in the sample but may also allow discovery of novel organisms...
December 2016: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/28098264/assessment-of-coulometric-array-electrochemical-detection-coupled-with-hplc-uv-for-the-absolute-quantitation-of-pharmaceuticals
#8
Michael B Hicks, Leah Salituro, Ian Mangion, Wes Schafer, Rong Xiang, Xiaoyi Gong, Christopher J Welch
The use of a coulometric array detector in tandem with HPLC-UV was evaluated for the absolute quantitation of pharmaceutical compounds without standards, an important capability gap in contemporary pharmaceutical research and development. The high-efficiency LC flow-through electrochemical detector system allows for the rapid evaluation of up to 16 different potentials, aiding in the identification and quantitation of electrochemically reactive species. By quantifying the number of electrons added or removed from an analyte during its passage through the detector, the number of moles of the analyte can be established...
January 18, 2017: Analyst
https://www.readbyqxmd.com/read/28096061/connexin37-deficiency-alters-organic-bone-matrix-cortical-bone-geometry-and-increases-wnt-%C3%AE-catenin-signaling
#9
Rafael Pacheco-Costa, Jay R Kadakia, Emily G Atkinson, Joseph M Wallace, Lilian I Plotkin, Rejane D Reginato
Deletion of connexin (Cx) 37 in mice leads to increased cancellous bone mass due to defective osteoclast differentiation. Paradoxically; however, Cx37-deficient mice exhibit reduced cortical thickness accompanied by higher bone strength, suggesting a contribution of Cx37 to bone matrix composition. Thus, we investigated whether global deletion of Cx37 alters the composition of organic bone extracellular matrix. Five-month-old Cx37(-/-) mice exhibited increased marrow cavity area, and periosteal and endocortical bone surface resulting in higher total area in tibia compared to Cx37(+/+) control mice...
January 13, 2017: Bone
https://www.readbyqxmd.com/read/28094514/structural-model-of-the-tubular-assembly-of-the-rous-sarcoma-virus-capsid-protein
#10
Jaekyun Jeon, Xin Qiao, Ivan Hung, Alok K Mitra, Ambroise Desfosses, Daniel Huang, Peter L Gor'kov, Rebecca C Craven, Richard L Kingston, Zhehong Gan, Fangqiang Zhu, Bo Chen
The orthoretroviral capsid protein (CA) assembles into polymorphic capsids, whose architecture, assembly and stability are still being investigated. The N-terminal and C-terminal domains of CA (NTD and CTD, respectively) engage in both homotypic and heterotypic interactions to create the capsid. Hexameric turrets formed by the NTD decorate the majority of the capsid surface. We report nearly-complete solid-state NMR (ssNMR) resonance assignments of Rous sarcoma virus (RSV) CA, assembled into hexamer tubes that mimic the authentic capsid...
January 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28092083/role-of-dopamine-d2-d3-receptors-in-development-plasticity-and-neuroprotection-in-human-ipsc-derived-midbrain-dopaminergic-neurons
#11
Federica Bono, Paola Savoia, Adele Guglielmi, Massimo Gennarelli, Giovanna Piovani, Sandra Sigala, Damiana Leo, Stefano Espinoza, Raul R Gainetdinov, Paola Devoto, PierFranco Spano, Cristina Missale, Chiara Fiorentini
The role of dopamine D2 and D3 receptors (D2R/D3R), located on midbrain dopaminergic (DA) neurons, in the regulation of DA synthesis and release and in DA neuron homeostasis has been extensively investigated in rodent animal models. By contrast, the properties of D2R/D3R in human DA neurons have not been elucidated yet. On this line, the use of human-induced pluripotent stem cells (hiPSCs) for producing any types of cells has offered the innovative opportunity for investigating the human neuronal phenotypes at the molecular levels...
January 14, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28091881/identifying-metabolites-of-meclonazepam-by-high-resolution-mass-spectrometry-using-human-liver-microsomes-hepatocytes-a-mouse-model-and-authentic-urine-samples
#12
Svante Vikingsson, Ariane Wohlfarth, Mikael Andersson, Henrik Gréen, Markus Roman, Martin Josefsson, Fredrik C Kugelberg, Robert Kronstrand
Meclonazepam is a benzodiazepine patented in 1977 to treat parasitic worms, which recently appeared as a designer benzodiazepine and drug of abuse. The aim of this study was to identify metabolites suitable as biomarkers of drug intake in urine using high-resolution mass spectrometry, authentic urine samples, and different model systems including human liver microsomes, cryopreserved hepatocytes, and a mice model. The main metabolites of meclonazepam found in human urine were amino-meclonazepam and acetamido-meclonazepam; also, minor peaks for meclonazepam were observed in three of four urine samples...
January 13, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28090732/high-engagement-high-quality-a-guiding-framework-for-developing-empirically-informed-asynchronous-e-learning-programs-for-health-professional-educators
#13
Peter M Sinclair, Tracey Levett-Jones, Amanda Morris, Ben Carter, Paul N Bennett, Ashley Kable
E-learning involves the transfer of skills and knowledge via technology so that learners can access meaningful and authentic educational materials. While learner engagement is important, in the context of healthcare education, pedagogy must not be sacrificed for edu-tainment style instructional design. Consequently, health professional educators need to be competent in the use of current web-based educational technologies so that learners are able to access relevant and engaging e-learning materials without restriction...
January 16, 2017: Nursing & Health Sciences
https://www.readbyqxmd.com/read/28090174/can-we-improve-training-for-health-professionals-to-sustain-local-health-development
#14
EDITORIAL
Stephen King
Can we improve training for health professionals? We explore specific variables that need to be accounted for to achieve sustainable local health development through training. A problem-based approach with appreciation of the need for making changes is suggested as the only authentic basis for training.
November 2016: Malaysian Journal of Medical Sciences: MJMS
https://www.readbyqxmd.com/read/28089912/hiding-clinical-information-in-medical-images-a-new-high-capacity-and-reversible-data-hiding-technique
#15
Shabir A Parah, Farhana Ahad, Javaid A Sheikh, G M Bhat
A new high capacity and reversible data hiding scheme for e-healthcare applications has been presented in this paper. Pixel to Block (PTB) conversion technique has been used as an effective and computationally efficient alternative to interpolation for the cover image generation to ensure reversibility of medical images. A fragile watermark and Block Checksum (computed for each 4 × 4 block) have been embedded in the cover image for facilitating tamper detection and tamper localization, and hence content authentication at receiver...
January 12, 2017: Journal of Biomedical Informatics
https://www.readbyqxmd.com/read/28088897/multiplex-pcr-as-a-rapid-and-sensitive-method-for-identification-of-meat-species-in-halal-meat-products
#16
Mahsa Alikord, Javad Keramat, Mahdi Kadivar, Hassan Momtaz, Mohammad Naghi Eshtiaghi, Aziz Homayouni Rad
BACKGROUND: Species identification and authentication in meat products are important subjects for ensuring the health of consumers. The multiplex-PCR amplification and species-specific primer set were used for identification of horse, donkey, pig and other ruminants in raw and processed meat products. METHODS: Oligonucleotid primers were designed for amplification of species-specific mitochondrial DNA sequences of each species and samples were prepared from binary meat mixtures...
January 13, 2017: Recent Patents on Food, Nutrition & Agriculture
https://www.readbyqxmd.com/read/28088859/determination-of-the-geographical-origin-of-all-commercial-hake-species-by-stable-isotope-ratio-sir-analysis
#17
Mónica Carrera, José M Gallardo
The determination of the geographical origin of food products is relevant to comply with the legal regulations of traceability, avoid food fraud and guarantee the food quality and safety to the consumers. For these reasons, Stable Isotope Ratio (SIR) analysis using an isotope ratio mass spectrometry (IRMS) instrument is one of the most useful technique for evaluating food traceability and authenticity. The present study was aimed to determine for the first time, the geographical origin for all commercial fish species belonging to the Merlucciidae family using SIR analysis of carbon (δ13C) and nitrogen (δ15N)...
January 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28088415/qualitative-and-quantitative-adulteration-identification-of-milk-powder-using-the-dna-with-novel-extraction-method
#18
J Liao, Y F Liu, T Ku, M H Liu, Y Huang
The extraction of high-quality DNA from processed dairy products can often represent the crucial step in an authentication process by PCR-based methods. In this study, we optimized a novel DNA extraction method for milk powder and used the extracted DNA for identification of milk powder based on PCR analysis. The DNA quality was assessed by amplifying target sequences from mitochondrial genes, as well as by monitoring the yield, purity, and integrity of the extracted DNA. In addition, a laboratory adulteration model of milk powder was detected by PCR-based methods (PCR and real-time PCR) using primers targeting the mitochondrial 12S rRNA gene...
January 11, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28088294/suitability-of-small-diagnostic-peripheral-blood-samples-for-cell-therapy-studies
#19
Coralea Stephanou, Panayiota Papasavva, Myria Zachariou, Petros Patsali, Marilena Epitropou, Petros Ladas, Ruba Al-Abdulla, Soteroulla Christou, Michael N Antoniou, Carsten W Lederer, Marina Kleanthous
BACKGROUND AIMS: Primary hematopoietic stem and progenitor cells (HSPCs) are key components of cell-based therapies for blood disorders and are thus the authentic substrate for related research. We propose that ubiquitous small-volume diagnostic samples represent a readily available and as yet untapped resource of primary patient-derived cells for cell- and gene-therapy studies. METHODS: In the present study we compare isolation and storage methods for HSPCs from normal and thalassemic small-volume blood samples, considering genotype, density-gradient versus lysis-based cell isolation and cryostorage media with different serum contents...
February 2017: Cytotherapy
https://www.readbyqxmd.com/read/28088059/infrared-analysis-of-clay-bricks-incorporated-with-spent-shea-waste-from-the-shea-butter-industry
#20
A N Adazabra, G Viruthagiri, N Shanmugam
The peculiar challenge of effective disposing abundant spent shea waste and the excellent compositional variation tolerance of clay material offered an impetus to examine the incorporation of spent shea waste into clay material as an eco-friendly disposal route in making clay bricks. For this purpose, the chemical constituent, mineralogical compositions and thermal behavior of both clay material and spent shea waste were initially characterized from which modelled brick specimens incorporating 5-20 wt% of the waste into the clay material were prepared...
January 11, 2017: Journal of Environmental Management
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