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https://www.readbyqxmd.com/read/28527183/analytical-methods-in-tracing-honey-authenticity
#1
Jelena Trifković, Filip Andrić, Petar Ristivojević, Etil Guzelmeric, Erdem Yesilada
Honey is a precious natural product that is marketed with a wide range of nutritional and medicinal properties. However, it is also a product subjected to frequent adulteration through mislabeling and mixing with cheaper and lower-quality honeys and various sugar syrups. In that sense, honey authentication regarding its genuine botanical and geographical origins, as well as the detection of any adulteration, is essential in order to protect consumer health and to avoid competition that could create a destabilized market...
May 19, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28527180/assessment-of-the-authenticity-of-honey
#2
Dušanka M Milojković-Opsenica, Živoslav Lj Tešić
No abstract text is available yet for this article.
May 19, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28526663/effects-of-using-child-personas-in-the-development-of-a-digital-peer-support-service-for-childhood-cancer-survivors
#3
Pontus Wärnestål, Petra Svedberg, Susanne Lindberg, Jens M Nygren
BACKGROUND: Peer support services have the potential to support children who survive cancer by handling the physical, mental, and social challenges associated with survival and return to everyday life. Involving the children themselves in the design process allows for adapting services to authentic user behaviors and goals. As there are several challenges that put critical requirements on a user-centered design process, we developed a design method based on personas adapted to the particular needs of children that promotes health and handles a sensitive design context...
May 18, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/28525869/hepatic-microsomal-metabolism-of-bde-47-and-bde-99-by-lesser-snow-geese-and-japanese-quail
#4
Lisa K Krieger, András Szeitz, Stelvio M Bandiera
In the present study, we investigated the oxidative biotransformation of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and 2,2',4,4',5-pentabromodiphenyl ether (BDE-99) by liver microsomes from wild lesser snow geese (Chen caerulescens caerulescens) and domesticated Japanese quail (Coturnix japonica). Formation of hydroxy-metabolites was analyzed using an ultra-high performance liquid chromatography-tandem mass spectrometry-based method. Incubation of BDE-47 with avian liver microsomes produced sixteen hydroxy-metabolites, eight of which were identified using authentic standards...
May 10, 2017: Chemosphere
https://www.readbyqxmd.com/read/28525847/apoptosis-of-leukemia-k562-and-molt-4-cells-induced-by-emamectin-benzoate-involving-mitochondrial-membrane-potential-loss-and-intracellular-ca-2-modulation
#5
Xinming Yun, Wenbing Rao, Ciying Xiao, Qingchun Huang
Leukemia threatens millions of people's health and lives, and the pesticide-induced leukemia has been increasingly concerned because of the etiologic exposure. In this paper, cytotoxic effect of emamectin benzoate (EMB), an excellent natural-product insecticide, was evaluated through monitoring cell viability, cell apoptosis, mitochondrial membrane potential and intracellular Ca(2+) concentration ([Ca(2+)]i) in leukemia K562 and Molt-4 cells. Following the exposure to EMB, cell viability was decreased and positive apoptosis of K562 and Molt-4 cells was increased in a concentration- and time- dependent fashion...
April 19, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28522732/unbiased-proteomics-of-early-lewy-body-formation-model-implicates-active-microtubule-affinity-regulating-kinases-marks-in-synucleinopathies
#6
Michael X Henderson, Charlotte Hiu-Yan Chung, Dawn M Riddle, Bin Zhang, Ronald J Gathagan, Steven H Seeholzer, John Q Trojanowski, Virginia M Y Lee
Parkinson's disease (PD) patients progressively accumulate intracytoplasmic inclusions formed by misfolded α-synuclein known as Lewy bodies (LBs). LBs also contain other proteins that may or may not be relevant in the disease process. To identify proteins involved early in LB formation, we performed proteomic analysis of insoluble proteins in a primary neuron culture model of α-synuclein pathology. We identified proteins previously found in authentic LBs in PD as well as several novel proteins, including the microtubule affinity-regulating kinase 1 (MARK1), one of the most enriched proteins in this model of LB formation...
May 17, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28521385/-ziziphus-oxyphylla-ethnobotanical-ethnopharmacological-and-phytochemical-review
#7
REVIEW
Rizwan Ahmad, Niyaz Ahmad, Atta Abbas Naqvi
ETHNOPHARMACOLOGICAL RELEVANCE: Ziziphus oxyphylla (ZO) is distributed mainly in tropic and warm temperate regions in the world. Pakistan owns six (06) indigenous species of genus Ziziphus out of which ZO is widely used for traditional treatment of different ailments such as diabetes, jaundice and liver diseases. AIM OF THE STUDY: The present review aims to provide in-depth and comprehensive literature overview, regarding botanical, chemical and biological characteristics of the plant alongwith phytochemical isolation and mechanistic studies to support its folklore and traditional uses...
May 14, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28520403/integrated-strategy-for-unknown-ei-ms-identification-using-quality-control-calibration-curve-multivariate-analysis-ei-ms-spectral-database-and-retention-index-prediction
#8
Teruko Matsuo, Hiroshi Tsugawa, Hiromi Miyagawa, Eiichiro Fukusaki
Compound identification using unknown electron ionization (EI) mass spectra in gas chromatography coupled with mass spectrometry (GC-MS) is challenging in untargeted metabolomics, natural product chemistry, or exposome research. While the total count of EI-MS records included in publicly or commercially available databases is over 900,000, efficient use of this huge database has not been achieved in metabolomics. Therefore, we proposed a 'four-step' strategy for the identification of biologically significant metabolites using an integrated cheminformatics approach: (i) Quality control calibration curve to reduce background noise, (ii) variable selection by hypothesis testing in principal component analysis for the efficient selection of target peaks, (iii) searching the EI-MS spectral database, and (iv) retention index (RI) filtering in combination with RI predictions...
May 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28514438/conversion-of-adult-endothelium-to-immunocompetent-haematopoietic-stem-cells
#9
Raphael Lis, Charles C Karrasch, Michael G Poulos, Balvir Kunar, David Redmond, Jose G Barcia Duran, Chaitanya R Badwe, William Schachterle, Michael Ginsberg, Jenny Xiang, Arash Rafii Tabrizi, Koji Shido, Zev Rosenwaks, Olivier Elemento, Nancy A Speck, Jason M Butler, Joseph M Scandura, Shahin Rafii
Developmental pathways that orchestrate the fleeting transition of endothelial cells into haematopoietic stem cells remain undefined. Here we demonstrate a tractable approach for fully reprogramming adult mouse endothelial cells to haematopoietic stem cells (rEC-HSCs) through transient expression of the transcription-factor-encoding genes Fosb, Gfi1, Runx1, and Spi1 (collectively denoted hereafter as FGRS) and vascular-niche-derived angiocrine factors. The induction phase (days 0-8) of conversion is initiated by expression of FGRS in mature endothelial cells, which results in endogenous Runx1 expression...
May 17, 2017: Nature
https://www.readbyqxmd.com/read/28512524/promoting-the-multidimensional-character-of-scientific-reasoning
#10
William S Bradshaw, Jennifer Nelson, Byron J Adams, John D Bell
This study reports part of a long-term program to help students improve scientific reasoning using higher-order cognitive tasks set in the discipline of cell biology. This skill was assessed using problems requiring the construction of valid conclusions drawn from authentic research data. We report here efforts to confirm the hypothesis that data interpretation is a complex, multifaceted exercise. Confirmation was obtained using a statistical treatment showing that various such problems rank students differently-each contains a unique set of cognitive challenges...
April 2017: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/28511609/why-the-history-of-nursing-ethics-matters
#11
Marsha D Fowler
Modern American nursing has an extensive ethical heritage literature that extends from the 1870s to 1965 when the American Nurses Association issued a policy paper that called for moving nursing education out of hospital diploma programs and into colleges and universities. One consequence of this move was the dispersion of nursing libraries and the loss of nursing ethics textbooks, as they were largely not brought over into the college libraries. In addition to approximately 100 nursing ethics textbooks, the nursing ethics heritage literature also includes hundreds of journal articles that are often made less accessible in modern databases that concentrate on the past 20 or 30 years...
May 2017: Nursing Ethics
https://www.readbyqxmd.com/read/28510572/1-2-million-kids-and-counting-mobile-science-laboratories-drive-student-interest-in-stem
#12
Amanda L Jones, Mary K Stapleton
In today's increasingly technological society, a workforce proficient in science, technology, engineering, and mathematics (STEM) skills is essential. Research has shown that active engagement by K-12 students in hands-on science activities that use authentic science tools promotes student learning and retention. Mobile laboratory programs provide this type of learning in schools and communities across the United States and internationally. Many programs are members of the Mobile Lab Coalition (MLC), a nonprofit organization of mobile and other laboratory-based education programs built on scientist and educator collaborations...
May 2017: PLoS Biology
https://www.readbyqxmd.com/read/28509839/autonomy-authenticity-and-deferred-decision-making-the-practical-application-of-abstract-concepts-for-physician-assistants
#13
Anna Skold, Jason Lesandrini
No abstract text is available yet for this article.
June 2017: Journal of Physician Assistant Education
https://www.readbyqxmd.com/read/28508779/ad-hoc-participation-in-professional-tele-collaboration-platforms
#14
Martin Staemmler, Barbara Rimmler, Heiko Münch, Uwe Engelmann, Johannes Sturm
Tele-collaboration between medical professionals is well established for specialties like radiology, cardiology or pathology. Typically these applications do not allow for ad hoc participation of non-registered users like patients and / or medical professionals. This paper describes an approach for extending these tele-applications to non-registered users and providing ad hoc participation with means for providing and accessing data. A light weight, web-based approach is used to assure acceptance and ease of use while maintaining and complying to the required regulations regarding authentication, authorization and protection of personal data...
2017: Studies in Health Technology and Informatics
https://www.readbyqxmd.com/read/28508213/reverse-genetics-of-zika-virus
#15
Chao Shan, Xuping Xie, Pei-Yong Shi
We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A T7 promoter was engineered in front of the viral 5' UTR for in vitro transcription. A hepatitis delta virus ribozyme (HDVr) sequence was engineered following the viral 3' UTR for generation of the authentic 3' end of the RNA transcript...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28507820/self-centeredness-and-selflessness-happiness-correlates-and-mediating-psychological-processes
#16
Michael Dambrun
The main objective of this research was to test central assumptions from the Self-centeredness/Selflessness Happiness Model. According to this model, while self-centered psychological functioning induces fluctuating happiness, authentic-durable happiness results from selflessness. Distinct mediating processes are supposed to account for these relationships: afflictive affects (e.g., anger, fear, jealousy, frustration) in the case of the former, and both emotional stability and feelings of harmony in the case of the latter...
2017: PeerJ
https://www.readbyqxmd.com/read/28506126/intergenerational-programs-may-be-especially-engaging-for-aged-care-residents-with-cognitive-impairment-findings-from-the-avondale-intergenerational-design-challenge
#17
Jess Rose Baker, Lindl Webster, Nigel Lynn, Julie Rogers, Jessica Belcher
Intergenerational programs are an authentic way to engage elders in meaningful activity and report benefits to both elders and youth. The Avondale Intergenerational Design Challenge (AVID) randomly assigned small teams of technology students aged 13 to 15 years (total N = 59) to 1 of 24 aged care residents with a range of cognitive impairment. Students met with the resident 4 times over 15 weeks and ultimately crafted a personalized item for them. Students showed no change in self-reported attitudes to elders, empathy, or self-esteem post-AVID or at 3-month follow-up, compared to a 3-month within-subject control period pre-AVID...
June 2017: American Journal of Alzheimer's Disease and Other Dementias
https://www.readbyqxmd.com/read/28505589/multi-platform-metabolomics-and-a-genetic-approach-support-the-authentication-of-agarwood-produced-by-aquilaria-crassna-and-aquilaria-malaccensis
#18
Huy Truong Nguyen, Jung-Eun Min, Nguyen Phuoc Long, Ma Chi Thanh, Thi Hong Van Le, Jeongmi Lee, Jeong Hill Park, Sung Won Kwon
Agarwood, the resinous heartwood produced by some Aquilaria species such as Aquilaria crassna, Aquilaria malaccensis and Aquilaria sinensis, has been traditionally and widely used in medicine, incenses and especially perfumes. However, up to now, the authentication of agarwood has been largely based on morphological characteristics, a method which is prone to errors and lacks reproducibility. Hence, in this study, we applied metabolomics and a genetic approach to the authentication of two common agarwood chips, those produced by Aquilaria crassna and Aquilaria malaccensis...
May 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28505414/quantitative-on-line-liquid-chromatography-surface-enhanced-raman-scattering-lc-sers-of-methotrexate-and-its-major-metabolites
#19
Abdu Subaihi, Drupad K Trivedi, Katherine Anne Hollywood, James Bluett, Yun Xu, Howbeer Muhamadali, David I Ellis, Royston Goodacre
The application of Raman spectroscopy as a detection method coupled with liquid chromatography (LC) has recently attracted considerable interest, although this has currently been limited to isocratic elution. The combination of LC with rapidly advancing Raman techniques, such as surface-enhanced Raman scattering (SERS), allows for the rapid separation, identification and quantification, leading to quantitative discrimination of closely eluting analytes. This study has demonstrated the utility of SERS in conjunction with reversed phase liquid chromatography (RP-LC), for the detection and quantification of the therapeutically relevant drug molecule methotrexate (MTX) and its metabolites 7-hydroxy methotrexate and 2,4-diamino-N(10)-methylpteroic acid (DAMPA) in pure solutions and mixtures, including spikes into human urine from a healthy individual...
May 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28503047/an-updated-phytopharmacological-review-on-medicinal-plant-of-arab-region-apium-graveolens-linn
#20
REVIEW
Abdulrahman Khazim Al-Asmari, Md Tanwir Athar, Saeed G Kadasah
Apium graveolens Linn. (Karafs) is used in traditional medicine for the treatment of the various ailments. There is a need to explore and authenticate the pharmacological profile and medicinal importance of the Karafs. In this paper, the literature and the published work on Apium were collected using online resources "Google scholar", "Web of science", "Scopus" and "PubMed". Each of the pharmacological activity was searched individually using the keywords "Apium/Karafs/Apium graveolens + individual pharmacological activity"...
January 2017: Pharmacognosy Reviews
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