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Samuel Mesihää, Ilpo Rasanen, Ilkka Ojanperä
Gas chromatography (GC) hyphenated with nitrogen chemiluminescence detection (NCD) and quadrupole time-of-flight mass spectrometry (QTOFMS) was applied for the first time to the quantitative analysis of new psychoactive substances (NPS) in urine, based on the N-equimolar response of NCD. A method was developed and validated to estimate the concentrations of three metabolites of the common stimulant NPS α-pyrrolidinovalerophenone (α-PVP) in spiked urine samples, simulating an analysis having no authentic reference standards for the metabolites and using the parent drug instead for quantitative calibration...
February 27, 2018: Forensic Science International
Shuming Qiu, Guoai Xu, Haseeb Ahmad, Yanhui Guo
The Session Initiation Protocol (SIP) is an extensive and esteemed communication protocol employed to regulate signaling as well as for controlling multimedia communication sessions. Recently, Kumari et al. proposed an improved smart card based authentication scheme for SIP based on Farash's scheme. Farash claimed that his protocol is resistant against various known attacks. But, we observe some accountable flaws in Farash's protocol. We point out that Farash's protocol is prone to key-compromise impersonation attack and is unable to provide pre-verification in the smart card, efficient password change and perfect forward secrecy...
2018: PloS One
Atul Kabra, Rohit Sharma, Shivali Singla, Ruchika Kabra, Uttam Singh Baghel
BACKGROUND: Myrica esculenta (Family: Myricaceae) commonly known as Kaiphala or Katphala is a widely used medicinal plant in Ayurveda. In spite of its numerous medicinal attributes, no published work is available till date on pharmacognostical characterization and HPTLC analysis of its leaves. OBJECTIVES: To investigate the pharmacognostical, physicochemical, and HPTLC profiles of M. esculenta leaves. MATERIALS AND METHODS: The measures taken for pharmacognostical characterization were organoleptic study, macroscopy, microscopy, powder microscopy, leaf constant, fluorescence analysis, preliminary phytochemical screening and HPTLC spectra profile...
March 12, 2018: Journal of Ayurveda and Integrative Medicine
Heidi Lourens, Brian Watermeyer, Leslie Swartz
PURPOSE: Scholars agree that effective rehabilitation relies on a bedrock of reciprocity, relational trust, and authenticity. It is therefore essential for practitioners to develop insight into the complex dynamics within helping relationships. This study aims to provide an in-depth understanding of visually impaired students' experience of informal helping relationships. METHODS: Ten visually impaired students at a South African university participated in one of two semi-structured focus group interviews (six and four in each group, respectively) wherein we explored their experience of informal helping relationships...
March 16, 2018: Disability and Rehabilitation
Mei Wang, Vijayasankar Raman, Jianping Zhao, Bharathi Avula, Yan-Hong Wang, Philip L Wylie, Ikhlas A Khan
Correct identification of the true bay leaf ( Laurus nobilis ) and its substitutes is important not only for the quality control of the products, but also for the safety of the consumers. L. nobilis is often substituted or confused with other species, such as Cinnamomum tamala, Pimenta racemosa, Syzygium polyanthum , and Umbellularia californica. In the present study, the potential of gas chromatography combined with quadrupole time-of-flight mass spectrometry for the profiling of various bay leaf products was evaluated for the first time...
March 14, 2018: Planta Medica
Gisleangela Lima Rodrigues Carrara, Andrea Bernardes, Alexandre Pazetto Balsanelli, Silvia Helena Henriques Camelo, Carmen Silvia Gabriel, Ariane Cristina Barboza Zanetti
OBJECTIVE: To identify the available scientific evidence about the use of instruments for the evaluation of leadership in health and nursing services and verify the use of leadership styles/models/theories in the construction of these tools. METHOD: Integrative literature review of indexed studies in the LILACS, PUBMED, CINAHL and EMBASE databases from 2006 to 2016. RESULTS: Thirty-eight articles were analyzed, exhibiting 19 leadership evaluation tools; the most used were the Multifactor Leadership Questionnaire, the Global Transformational Leadership Scale, the Leadership Practices Inventory, the Servant Leadership Questionnaire, the Servant Leadership Survey and the Authentic Leadership Questionnaire...
March 12, 2018: Revista Gaúcha de Enfermagem
Muhammad Sohail, Liangmin Wang
Today IoT integrate thousands of inter networks and sensing devices e.g., vehicular networks, which are considered to be challenging due to its high speed and network dynamics. The goal of future vehicular networks is to improve road safety, promote commercial or infotainment products and to reduce the traffic accidents. All these applications are based on the information exchange among nodes, so not only reliable data delivery but also the authenticity and credibility of the data itself are prerequisite. To cope with the aforementioned problem, trust management come up as promising candidate to conduct node's transaction and interaction management, which requires distributed mobile nodes cooperation for achieving design goals...
March 14, 2018: Sensors
Tomasz Stępkowski, Joanna Banasiewicz, Camille E Granada, Mitchell Andrews, Luciane M P Passaglia
The legume tribe Genisteae comprises 618, predominantly temperate species, showing an amphi-Atlantic distribution that was caused by several long-distance dispersal events. Seven out of the 16 authenticated rhizobial genera can nodulate particular Genisteae species. Bradyrhizobium predominates among rhizobia nodulating Genisteae legumes. Bradyrhizobium strains that infect Genisteae species belong to both the Bradyrhizobium japonicum and Bradyrhizobium elkanii superclades. In symbiotic gene phylogenies, Genisteae bradyrhizobia are scattered among several distinct clades, comprising strains that originate from phylogenetically distant legumes...
March 14, 2018: Genes
Natalie Colaneri, Mark Sheldon, Andrew Adesman
PURPOSE OF REVIEW: Given the pervasiveness of psychotropic medication in the youth population and an increasingly competitive culture regarding educational performance, children, teenagers, and/or their parents may increasingly seek psychotropic substances in an effort to enhance a student's cognitive abilities and/or academic performance. Physicians must become aware of this very important and clinically relevant issue and work to ensure that medications remain in the hands of patients seeking wellness and not enhancement...
March 13, 2018: Current Opinion in Pediatrics
Moran N Cabili, Knox Carey, Stephanie O M Dyke, Anthony J Brookes, Marc Fiume, Francis Jeanson, Giselle Kerry, Alex Lash, Heidi Sofia, Dylan Spalding, Anne-Marie Tasse, Susheel Varma, Ravi Pandya
The volume of genomics and health data is growing rapidly, driven by sequencing for both research and clinical use. However, under current practices, the data is fragmented into many distinct datasets, and researchers must go through a separate application process for each dataset. This is time-consuming both for the researchers and the data stewards, and it reduces the velocity of research and new discoveries that could improve human health. We propose to simplify this process, by introducing a standard Library Card that identifies and authenticates researchers across all participating datasets...
March 14, 2018: Scientific Data
Oksana V Bocharova, Sentyabrina P Reshta, Maiia Y Bocharova, Vasiliy I Eshtokin
BACKGROUND: Organoleptic properties, and positive health effect on consumers, both justify the importance of the determination of apple juice authenticity, in respect of retail and wholesale trade networks worldwide. RESULTS: Adulteration of apple juice through dilution with water, and with ascorbic acid has been determined. It has been demonstrated that polarization curves of platinum electrode (Pt) in apple juice are useful for undeclared antioxidant determination, due to the movement of the polarization curve to the left, by 200 mV, when ascorbic acid was added...
March 13, 2018: Journal of the Science of Food and Agriculture
Li Yang, Zhiming Zheng
According to advancements in the wireless technologies, study of biometrics-based multi-server authenticated key agreement schemes has acquired a lot of momentum. Recently, Wang et al. presented a three-factor authentication protocol with key agreement and claimed that their scheme was resistant to several prominent attacks. Unfortunately, this paper indicates that their protocol is still vulnerable to the user impersonation attack, privileged insider attack and server spoofing attack. Furthermore, their protocol cannot provide the perfect forward secrecy...
2018: PloS One
Walter W Chen, Elizaveta Freinkman, David M Sabatini
Mitochondria carry out numerous metabolic reactions that are critical to cellular homeostasis. Here we present a protocol for interrogating mitochondrial metabolites and measuring their matrix concentrations. Our workflow uses high-affinity magnetic immunocapture to rapidly purify HA-tagged mitochondria from homogenized mammalian cells in ∼12 min. These mitochondria are extracted with methanol and water. Liquid chromatography and mass spectrometry (LC/MS) is used to determine the identities and mole quantities of mitochondrial metabolites using authentic metabolite standards and isotopically labeled internal standards, whereas the corresponding mitochondrial matrix volume is determined via immunoblotting, confocal microscopy of intact cells, and volumetric analysis...
October 2017: Nature Protocols
Stefanie Wernisch, Farsad Afshinnia, Thekkelnaycke Rajendiran, Subramaniam Pennathur
Metabolomics applications of differential mobility spectrometry (DMS)-mass spectrometry (MS) have largely concentrated on targeted assays and the removal of isobaric or chemical interferences from the signals of a small number of analytes. In the work reported here, we systematically investigated the application range of a DMS-MS method for metabolomics using more than 800 authentic metabolite standards as the test set. The coverage achieved with the DMS-MS platform was comparable to that achieved with chromatographic methods...
March 12, 2018: Analytical and Bioanalytical Chemistry
Joanne Rowe, Chrystal Jaye
INTRODUCTION Intentional self-harm is an international public health issue with high personal, social and financial costs to society. Poor relationship dynamics are known to have a negative influence on the psyche of people who self-harm, and this can increase anxiety and decrease self-esteem, both shown to be significant contributors to self-harm behaviours. Positive and functional social supports have been proposed as a cost-effective and constructive approach in diminishing self-harming behaviours. AIM This qualitative study investigated the aspects of professional, social, familial and romantic relationships that people who have self-harmed identified as having a positive and constructive effect on their self-harm behaviour...
December 2017: Journal of Primary Health Care
Anders Helander, Katarina Stojanovic, Tomas Villén, Olof Beck
In recent time, structural variants of fentanyl ("designer fentanyls") have appeared on the recreational drug market for new psychoactive substances (NPS). These potent opioids have caused harmful intoxications and increased opioid related mortality in many countries. This work evaluated three commercial immunoassays for fentanyl screening in urine, and investigated whether they are useful also for screening of designer fentanyls. The assays examined were the Thermo DRI® Fentanyl Enzyme Immunoassay, the ARK™ Fentanyl Assay homogeneous enzyme immunoassay, and the Immunalysis® Fentanyl Urine SEFRIA™ Drug Screening Kit...
March 12, 2018: Drug Testing and Analysis
Anna Puganen, Heikki Kallio, K M Schaich, Jukka-Pekka Suomela, Baoru Yang
The potential for using extracts of press residues from black, green, red and white currants and from sea buckthorn berries as sources of antioxidants for foods use was investigated. Press residues were extracted with ethanol in four consecutive extractions, and total Folin-Ciocalteu (F-C) reactive material and authentic phenolic compounds were determined. Radical quenching capability and mechanisms were determined from TRAP and ORAC assays and from DPPH kinetics, respectively; specific activities were normalized to F-C reactive concentrations...
March 12, 2018: Journal of Agricultural and Food Chemistry
Anam Fatima, Muhammad Zafar, Mushtaq Ahmad, Ghulam Yaseen, Shazia Sultana, Muhammad Gulfraz, Amir Muhammad Khan
Now-a-days, plant species are consumed globally for various purposes and this increasing demand leads to adulteration due to gradually exploitation in natural resources. The major causes of adulteration may be confusion in nomenclature, unawareness of authentic sources, unavailability of authentic sources, color resemblances, deficiencies in collection procedures, and misidentification. This study aims to use the microscopic techniques such as scanning electron microscopy for the authentication of the oil yielding seeds of four important and traditionally used species Prunus persica, Prunus domestica, and Eruca sativa and Argemone Mexicana from their adulterants...
March 12, 2018: Microscopy Research and Technique
Jennifer R Mallette, John F Casale, Valerie L Colley, David R Morello, James Jordan
For nearly 30years, the methods utilized in illicit cocaine hydrochloride production have remained relatively consistent. Cocaine hydrochloride is typically produced one kilogram at a time. As a result, each individual kilogram is unique and distinct from other kilograms in any particular seizure based on the total alkaloid profile, occluded solvent profile, and isotopic signature. Additionally, multi-kilogram cocaine seizures are often comprised of cocaine from several different coca growing regions. There has been a documented shift in this type of processing based on the recent analysis of a large cocaine seizure in the Eastern Pacific...
March 2018: Science & Justice: Journal of the Forensic Science Society
Zhengjia Ren, Meng Gao, Mark Yang, Wei Qu
After Sichuan earthquake, a few dedicated teams of psychological volunteers have been committed to helping the survivors of the disaster for a long period. Their personal transformation experiences were absent in the literatures. The purpose of this qualitative research was to adopt a qualitative research to examine individuals' lived experiences of personal transformation after long-term disaster mental health services. The study interviewed 10 psychological counselors, 3 psychiatric nurses, 4 psychiatrist, and 6 social workers...
March 10, 2018: Journal of Religion and Health
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