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https://www.readbyqxmd.com/read/27859556/effect-of-nanosilver-on-cortisol-release-and-morphometrics-in-rainbow-trout-oncorhynchus-mykiss
#1
Laura Murray, Michael D Rennie, Eva C Enders, Kerri Pleskach, Jonathan D Martin
Nanosilver (nAg) is a nanoparticle commonly incorporated into consumer products for its antimicrobial properties that has been detected in aquatic environments. Toxic effects of nAg on fish have been observed and may induce a stress response in fish in the form of increased blood plasma cortisol. In this study, effects of nAg exposure on Rainbow Trout (Oncorhynchus mykiss) were investigated over a 28 d period using blood plasma cortisol concentrations as an indicator of stress. Several morphometric measures (growth, Fulton's condition factor, and hepatosomatic index or HSI) were also taken during the experiment to investigate potential whole-body effects of exposure, and concentrations of nAg in fish muscle tissue were measured...
November 17, 2016: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/27834732/oxidant-generation-dna-damage-and-cytotoxicity-by-a-panel-of-engineered-nanomaterials-in-three-different-human-epithelial-cell-lines
#2
Waluree Thongkam, Kirsten Gerloff, Damien van Berlo, Catrin Albrecht, Roel P F Schins
Due to the steeply increased use of nanomaterials (NMs) for commercial and industrial applications, toxicological assessment of their potential harmful effects is urgently needed. In this study, we compared the DNA-damaging properties and concurrent cytotoxicity of a panel of 10 engineered NMs in three different cell lines in relation to their intrinsic oxidant generating properties. The human epithelial cell lines A549, HK-2 and HepG2 were chosen to represent relevant target organs for NMs in the lung, kidney and liver...
November 10, 2016: Mutagenesis
https://www.readbyqxmd.com/read/27825359/use-of-a-modified-greenscreen-tool-to-conduct-a-screening-level-comparative-hazard-assessment-of-conventional-silver-and-two-forms-of-nanosilver
#3
Jennifer Sass, Lauren Heine, Nina Hwang
BACKGROUND: Increased concern for potential health and environmental impacts of chemicals, including nanomaterials, in consumer products is driving demand for greater transparency regarding potential risks. Chemical hazard assessment is a powerful tool to inform product design, development and procurement and has been integrated into alternative assessment frameworks. The extent to which assessment methods originally designed for conventionally-sized materials can be used for nanomaterials, which have size-dependent physical and chemical properties, have not been well established...
November 8, 2016: Environmental Health: a Global Access Science Source
https://www.readbyqxmd.com/read/27800593/biocompatibility-of-nanosilver-coated-orthodontic-brackets-an-in-vivo-study
#4
Gamze Metin-Gürsoy, Lale Taner, Emre Barış
BACKGROUND: Nanosilver particles of which antibacterial and antifungal properties have been shown in various in vitro and in vivo studies are used in many medical and dental fields for the prevention of infection. In this study, it is intended to evaluate the biocompatibility of nanosilver-coated brackets. METHODS: Nanosilver coating process was applied to the standard orthodontic brackets by a physical vapor deposition system. Brackets were coated with nanosilver particles of 1 μ thickness...
December 2016: Progress in Orthodontics
https://www.readbyqxmd.com/read/27747311/antimicrobial-effects-of-silver-nanoparticles-stabilized-in-solution-by-sodium-alginate
#5
Anatoliy Kubyshkin, Denis Chegodar, Andrew Katsev, Armen Petrosyan, Yuri Krivorutchenko, Olga Postnikova
BACKGROUND/PURPOSE: To investigate the effect of nanosilver particles in solution stabilized in a matrix of sodium alginate on the growth and development of pathogenic bacteria such as Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Proteus vulgaris, Enterobacter cloacae, the antibiotic-resistant strain of Pseudomonas aeruginosa, the yeast-like fungus Candida albicans, and the luminescent bacteria Photobacterium leiognathi Sh1. METHODS: Isolates of pathogenic bacteria obtained from bronchoalveolar and peritoneal lavage samples from Wistar rats with experimental pneumonia and peritonitis were tested for their susceptibility to silver nanoparticles in solution with an alginate stabilizer...
2016: Biochemistry & Molecular Biology Journal
https://www.readbyqxmd.com/read/27721039/-gold-nanoparticles-composite-folic-acid-conjugated-graphene-oxide-nanohybrids-for-targeted-chemo-thermal-cancer-ablation-in-vitro-screening-and-in-vivo-studies
#6
Gaurav Chauhan, Vianni Chopra, Amit Tyagi, Goutam Rath, Rakesh K Sharma, Amit K Goyal
Nano-graphene oxide (GO) nanometal composite (specifically nanogold and nanosilver) have shown to be a promising material for anticancer therapeutics. Owing to their high drug loading capacity, photothermal and synergizing effects, it is very important to exploit them for targeted chemo-thermal cancer therapeutics. In this work, gold nanoparticles (AuNPs) were selected as the composite metal, folic acid (FA) was taken as GO surface functionalization moiety for active tumor targeting of model anticancer drug Doxorubicin (Dox)...
October 6, 2016: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27704758/additively-manufactured-macroporous-titanium-with-silver-releasing-micro-nanoporous-surface-for-multipurpose-infection-control-and-bone-repair-%C3%A2-a-proof-of-concept
#7
Zhaojun Jia, Peng Xiu, Pan Xiong, Wenhao Zhou, Yan Cheng, Shicheng Wei, Yufeng Zheng, Tingfei Xi, Hong Cai, Zhongjun Liu, Cai-Mei Wang, Wei-Ping Zhang, Zhi-Jiang Li
Restoring large-scale bone defects, where osteogenesis is slow while infections lurk, with biomaterials represents a formidable challenge in orthopedic clinics. Here, we propose a scaffold-based multipurpose anti-infection and bone repairing strategy to meet such restorative needs. To do this, personalized multifunctional titanium meshes were produced through an advanced additive manufacturing process and dual "TiO2‒poly(dopamine)/Ag (nano)" post modifications, yielding macroporous constructs with micro/nanoporous walls and nanosilver bullets immobilized/embedded therein...
October 5, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27699838/biomarkers-of-exposure-to-nanosilver-and-silver-accumulation-in-yellow-perch-perca-flavescens
#8
Jonathan D Martin, Tash-Lynn L Colson, Valerie S Langlois, Chris D Metcalfe
There is a risk of exposure of aquatic organisms to nanosilver (AgNP) from discharges of municipal and industrial wastewater. In the present study, yellow perch (Perca flavescens) were exposed in static-renewal experiments conducted over 96 h and 10 d to environmentally relevant concentrations (i.e. 1 and 100 µg/L) of AgNP and Ag(+) . The greatest accumulation of total silver (tAg) occurred in the liver of P. flavescens and there was >10-fold more accumulation in the treatments with Ag(+) relative to the AgNP treatments...
October 4, 2016: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/27693238/synthesis-and-characterization-of-nanosilver-silicone-hydrogel-composites-for-inhibition-of-bacteria-growth
#9
F M Helaly, S M El-Sawy, A I Hashem, A A Khattab, R M Mourad
PURPOSE: Nanosilver-silicone hydrogel (NAgSiH) composites for contact lenses were synthesized to asses the antimicrobial effects. METHODS: Silicone hydrogel (SiH) films were synthesized followed by impregnation in silver nitrate solutions (10, 20, 30, 40, 60, 80ppm) and in-situ chemical reduction of silver ions using sodium borohydride (NaBH4). The silver nano particles (AgNPS) were identified by UV-vis absorption spectroscopy, Energy-dispersive X-ray spectroscopy (EDX) mapping and EDX spectrum...
September 29, 2016: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/27676384/evaluation-of-the-wound-healing-efficacy-of-chemical-and-phytogenic-silver-nanoparticles
#10
Sreevani Pothireddy, Adilaxmamma Kaliki, Alpha Raj Mekapogu, Muralidhar Yegireddy, Eswara Prasad Pagadala, Tollamadugu Naga Venkata Krishna Vara Prasad
Wound healing requires a series of cellular events and a cascade of co-ordinated and systemic biochemical events. Silver nanoparticles possess many beneficial properties for wound management including antibacterial, anti-inflammatory and pro-healing properties. In this study, the authors investigated the wound healing properties of Cinnamomum verum extract mediated nanosilver (CENS) particles in comparison with 1% povidone iodine, citrate mediate NS and CE treatments. The topical application of CENS showed good antibacterial activity and accelerated wound healing with complete epithelialisation and normal re-growth of hair in all three models of study: namely, excision, incision and dead space models in rats compared with all other treatments...
October 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27676378/green-synthesis-of-nanosilver-decorated-graphene-oxide-sheets
#11
Claramaría Rodríguez-González, Pamela Velázquez-Villalba, Pedro Salas, Víctor M Castaño
A green facile method has been successfully used for the synthesis of graphene oxide sheets decorated with silver nanoparticles (rGO/AgNPs), employing graphite oxide as a precursor of graphene oxide (GO), AgNO3 as a precursor of Ag nanoparticles (AgNPs), and geranium (Pelargonium graveolens) extract as reducing agent. Synthesis was accomplished using the weight ratios 1:1 and 1:3 GO/Ag, respectively. The synthesised nanocomposites were characterised by scanning electron microscopy, transmission electron microscopy, atomic force microscopy, X-ray diffraction, UV-visible spectroscopy, Raman spectroscopy, energy dispersive X-ray spectroscopy and thermogravimetric analysis...
October 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27658822/fibrous-shape-underlies-the-mutagenic-and-carcinogenic-potential-of-nanosilver-while-surface-chemistry-affects-the-biosafety-of-iron-oxide-nanoparticles
#12
Alena Gábelová, Naouale El Yamani, Tamara Iglesias Alonso, Barbora Buliaková, Annamária Srančíková, Andrea Bábelová, Elise Runden Pran, Lise Marie Fjellsbø, Elisabeth Elje, Mazyar Yazdani, Maria João Silva, Mária Dušinská
Nowadays engineered nanomaterials (ENMs) are increasingly used in a wide range of commercial products and biomedical applications. Despite this, the knowledge of human potential health risk as well as comprehensive biological and toxicological information is still limited. We have investigated the capacity of two frequently used metallic ENMs, nanosilver and magnetite nanoparticles (MNPs), to induce thymidine kinase (Tk (+/-)) mutations in L5178Y mouse lymphoma cells and transformed foci in Bhas 42 cells. Two types of nanosilver, spherical nanoparticles (AgNM300) and fibrous (AgNM302) nanorods/wires, and MNPs differing in surface modifications [MNPs coated with sodium oleate (SO-MNPs), MNPs coated with SO + polyethylene glycol (SO-PEG-MNPs) and MNPs coated with SO + PEG + poly(lactide-co-glycolic acid) SO-PEG-PLGA-MNPs] were included in this study...
September 22, 2016: Mutagenesis
https://www.readbyqxmd.com/read/27642257/new-rapid-method-to-measure-dissolved-silver-concentration-in-silver-nanoparticle-suspensions-by-aggregation-combined-with-centrifugation
#13
Feng Dong, Eugenia Valsami-Jones, Jan-Ulrich Kreft
It is unclear whether the antimicrobial activities of silver nanoparticles (AgNPs) are exclusively mediated by the release of silver ions (Ag(+)) or, instead, are due to combined nanoparticle and silver ion effects. Therefore, it is essential to quantify dissolved Ag in nanosilver suspensions for investigations of nanoparticle toxicity. We developed a method to measure dissolved Ag in Ag(+)/AgNPs mixtures by combining aggregation of AgNPs with centrifugation. We also describe the reproducible synthesis of stable, uncoated AgNPs...
2016: Journal of Nanoparticle Research: An Interdisciplinary Forum for Nanoscale Science and Technology
https://www.readbyqxmd.com/read/27636544/varying-the-morphology-of-silver-nanoparticles-results-in-differential-toxicity-against-micro-organisms-hacat-keratinocytes-and-affects-skin-deposition
#14
Amy M Holmes, Julian Lim, Hauke Studier, Michael S Roberts
The use of silver nanoparticles (Ag NPs) within the healthcare sector and consumer products is rapidly increasing. There are now a range of diverse-shaped Ag NPs that are commercially available and many of the products containing nanosilver are topically applied to human skin. Currently, there is limited data on the extent to which the antimicrobial efficacy and cytotoxicity of Ag NPs is related to their shape and how the shape of the Ag NPs affects their distribution in both intact and burn wounded human skin after topical application...
October 5, 2016: Nanotoxicology
https://www.readbyqxmd.com/read/27636513/synthesis-of-stable-nanosilver-particles-agnps-by-the-proteins-of-seagrass-syringodium-isoetifolium-and-its-biomedicinal-properties
#15
N K Ahila, V Sri Ramkumar, S Prakash, B Manikandan, J Ravindran, P K Dhanalakshmi, E Kannapiran
A simple eco-friendly approach for the hasty synthesis of stable, potent and benign silver nanoparticles (AgNPs) using seagrass, Syringodium isoetifolium was proposed and described here. The UV-Vis, DLS, XRD, AFM, FESEM, EDX and HRTEM analysis highly characterized and confirmed the presence of polydispersed (2-50nm) spherical and stable AgNPs. FT-IR and phytochemical analysis suggested that the proteins act as reducing and also as capping agent. A hypothetical approach using bioinformatics tools revealed that the Phytochrome B protein of S...
September 13, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27624340/in-vitro-effects-of-hollow-gold-nanoshells-on-human-aortic-endothelial-cells
#16
Chunrong Gu, Hengfang Wu, Gaoyuan Ge, Xiongzhi Li, Zhirui Guo, Zhiping Bian, Jindan Xu, Hua Lu, Xiangjian Chen, Di Yang
Gold nanoparticles are emerging as promising biomedical tools due to their unique nanoscale characteristics. Our purpose was to synthesize a hollow-shaped gold nanoparticle and to investigate its effect on human aortic endothelial cells (HAECs) in vitro. Hollow gold nanoshells with average 35-nm diameters and 10-nm shell thickness were obtained by galvanic replacement using quasi-spherical nanosilver as sacrifice-template. Our results showed that hollow gold nanoshells in the culture medium could be internalized into the cytoplasm of HAECs...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27612801/skin-compatibility-and-antimicrobial-studies-on-biofunctionalized-polypropylene-fabric
#17
Sadiya Anjum, Amlan Gupta, Deepika Sharma, Prashansa Dalal, Bhuvanesh Gupta
The aim of this study was the development of antimicrobial fabric which can be used as skin contacting material. The nanosilver loaded bioactive nanogels of polyacrylamide were prepared by gamma irradiation process and the particle size was observed to be in the range of 10-50nm. In this study, we used polyethylene glycol as carrier for the combination of functional nanogel and essential oils together. Plasma functionalized polypropylene fabric was used as the base material for the bio-immobilization. Bioactive emulsion was coated on the fabric which exhibited excellent antimicrobial activity against Staphylococcus aureus and Escherichia coli...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27601426/comparative-genotoxicity-of-silver-nanoparticles-in-human-liver-hepg2-and-lung-epithelial-a549-cells
#18
J Wang, B Che, L W Zhang, G Dong, Q Luo, L Xin
With the rapid expanding of human exposure to silver nanoparticles (AgNPs), genotoxicity screening of nanosilver is necessary to ensure consumer safety. Here, we assessed one key DNA damage responsive pathway activated by GADD45a gene after 24 h of AgNPs exposure in stable luciferase reporter cell systems based on two widely used in vitro cell models, human liver HepG2 and lung epithelial A549 cells. The comet assay and micronucleus test were also conducted to confirm the genetic damage induced by AgNPs. Our results showed that AgNPs produced a strong dose-dependent increase in transcriptional activation of GADD45a promoter indicated by luciferase activity accompanying by the significant decreases in cell viability...
September 7, 2016: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/27581559/reusable-magnetic-nanobiocatalyst-for-synthesis-of-silver-and-gold-nanoparticles
#19
Jahirul Ahmed Mazumder, Razi Ahmad, Meryam Sardar
In the present work, we describe a simple procedure for the biosynthesis of nanosilver and gold by the reduction of silver nitrate and auric chloride respectively using a nanobiocatalyst. The nanobiocatalyst was prepared by covalent coupling of alpha amylase on (3-aminopropyl)triethoxysilane (APTES) modified iron oxide magnetic nanoparticles. The nanobiocatalyst retains 77% of its activity as compared to free alpha amylase. The nanobiocatalyst can be used up to three consecutive cycles for the synthesis of nano silver and gold...
August 28, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27577913/synthesis-of-nanosilver-loaded-chitosan-poly-acrylamide-co-itaconic-acid-based-inter-polyelectrolyte-complex-films-for-antimicrobial-applications
#20
S K Bajpai, Pooja Jyotishi, M Bajpai
In the present work, AgNPs loaded chitosan/poly(acrylamide-co-itaconic acid) inter-polyelectrolyte complex (IPC) films have been prepared for antimicrobial applications. The AgNPs-loaded IPC films have been characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Transmission electron microscopy (TEM), Scanning electron microscopy (SEM), Thermogravimetric analysis (TGA) and Surface plasmon resonance (SPR). Particle size of synthesized AgNPs was found to be in the range 10-30nm...
December 10, 2016: Carbohydrate Polymers
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