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https://www.readbyqxmd.com/read/29330021/ccn4-wisp1-controls-cutaneous-wound-healing-by-modulating-proliferation-migration-and-ecm-expression-in-dermal-fibroblasts-via-%C3%AE-5%C3%AE-1-and-tnf%C3%AE
#1
Mitsuaki Ono, Asuka Masaki, Azusa Maeda, Tina M Kilts, Emilio S Hara, Taishi Komori, Hai Pham, Takuo Kuboki, Marian F Young
Understanding the mechanisms that control cutaneous wound healing is crucial to successfully manage repair of damaged skin. The goal of the current study was to uncover novel extracellular matrix (ECM) components that control the wound healing process. Full thickness skin defects were created in mice and used to show CCN4 up-regulation during wound-healing as early as 1 day after surgery, suggesting a role in inflammation and subsequent dermal migration and proliferation. To determine how CCN4 could regulate wound healing we used Ccn4-KO mice and showed they had delayed wound closure accompanied by reduced expression of Col1a1 and Fn mRNA...
January 9, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29329516/bilateral-giant-retinal-tears-in-osteogenesis-imperfecta
#2
Paolo Scollo, Martin Paul Snead, Allan James Richards, Rebecca Pollitt, Catherine DeVile
BACKGROUND: Osteogenesis imperfecta (OI) is a rare primarily autosomal dominant condition in which the connective tissues of bones, ligaments and sclerae do not form properly. Typically, mutations in COL1A1 and COL1A2 genes lead to the defective formation or quantity of type I collagen, the principle matrix in these tissues. Molecular genetic studies have now elucidated multiple genetic subtypes of the disorder but little literature exists on the risk of retinal tears and detachments in OI...
January 12, 2018: BMC Medical Genetics
https://www.readbyqxmd.com/read/29325202/interfacial-mineral-fusion-and-tubule-entanglement-as-a-means-to-harden-a-bone-augmentation-material
#3
Erik A B Hughes, Sophie C Cox, Megan E Cooke, Owen G Davies, Richard L Williams, Thomas J Hall, Liam M Grover
A new bone augmenting material is reported, which is formed from calcium-loaded hydrogel-based spheres. On immersion of these spheres in a physiological medium, they become surrounded with a sheath of precipitate, which ruptures due to a build-up in osmotic pressure. This results in the formation of mineral tubes that protrude from the sphere surface. When brought into close contact with one another, these spheres become fused through the entanglement and subsequent interstitial mineralization of the mineral tubules...
January 11, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29322079/fibroblast-and-keratinocyte-gene-expression-following-exposure-to-extracts-of-neem-plant-azadirachta-indica
#4
Takao Someya, Katsura Sano, Kotaro Hara, Yoshimasa Sagane, Toshihiro Watanabe, R G S Wijesekara
This data article provides gene expression profiles, determined by using real-time PCR, of fibroblasts and keratinocytes treated with 0.01% and 0.001% extracts of neem plant (Azadirachta indica), local name "Kohomba" in Sri Lanka, harvested in Sri Lanka. For fibroblasts, the dataset includes expression profiles for genes encoding hyaluronan synthase 1 (HAS1), hyaluronan synthase 2 (HAS2), hyaluronidase-1 (HYAL1), hyaluronidase-2 (HYAL2), versican, aggrecan, CD44, collagen, type I, alpha 1 (COL1A1), collagen, type III, alpha 1 (COL3A1), collagen, type VII, alpha 1 (COL7A1), matrix metalloproteinase 1 (MMP1), acid ceramidase, basic fibroblast growth factor (bFGF), fibroblast growth factor-7 (FGF7), vascular endothelial growth factor (VEGF), interleukin-1 alpha (IL-1α), cyclooxygenase-2 (cox2), transforming growth factor beta (TGF-β), and aquaporin 3 (AQP3)...
February 2018: Data in Brief
https://www.readbyqxmd.com/read/29320421/comparative-analysis-of-different-platelet-lysates-and-platelet-rich-preparations-to-stimulate-tendon-cell-biology-an-in-vitro-study
#5
Franka Klatte-Schulz, Tanja Schmidt, Melanie Uckert, Sven Scheffler, Ulrich Kalus, Markus Rojewski, Hubert Schrezenmeier, Axel Pruss, Britt Wildemann
The poor healing potential of tendons is still a clinical problem, and the use of Platelet Rich Plasma (PRP) was hypothesized to stimulate healing. As the efficacy of PRPs remains unproven, platelet lysate (PL) could be an alternative with its main advantages of storage and characterization before use. Five different blood products were prepared from 16 male donors: human serum, two PRPs (Arthrex, (PRP-ACP); RegenLab (PRP-BCT)), platelet concentrate (apheresis, PC), and PL (freezing-thawing destruction of PC)...
January 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29315680/solexa-sequencing-and-custom-microrna-chip-reveal-repertoire-of-micrornas-in-mammary-gland-of-bovine-suffering-from-natural-infectious-mastitis
#6
Zhihua Ju, Qiang Jiang, Gang Liu, Xiuge Wang, Guojing Luo, Yan Zhang, Jibin Zhang, Jifeng Zhong, Jinming Huang
Identification of microRNAs (miRNAs), target genes and regulatory networks associated with innate immune and inflammatory responses and tissue damage is essential to elucidate the molecular and genetic mechanisms for resistance to mastitis. In this study, a combination of Solexa sequencing and custom miRNA chip approaches was used to profile the expression of miRNAs in bovine mammary gland at the late stage of natural infection with Staphylococcus aureus, a widespread mastitis pathogen. We found 383 loci corresponding to 277 known and 49 putative novel miRNAs, two potential mitrons and 266 differentially expressed miRNAs in the healthy and mastitic cows' mammary glands...
January 8, 2018: Animal Genetics
https://www.readbyqxmd.com/read/29314764/biomimetic-fetal-rotation-bioreactor-for-engineering-bone-tissues-effect-of-cyclic-strains-on-upregulation-of-osteogenic-gene-expression
#7
Akhilandeshwari Ravichandran, Feng Wen, Jing Lim, Mark Seow Khoon Chong, Jerry K Y Chan, Swee-Hin Teoh
Cells respond to physiological mechanical stresses especially during early fetal development. Adopting a biomimetic approach, it is necessary to develop bioreactor systems to explore the effects of physiologically relevant mechanical strains and shear stresses for functional tissue growth and development. This study introduces a multimodal bioreactor system that allows application of cyclic compressive strains on premature bone grafts that are cultured under biaxial rotation (chamber rotation about two axes) conditions for bone tissue engineering...
January 3, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29306778/influence-on-proliferation-and-adhesion-of-human-gingival-fibroblasts-from-different-titanium-surface-decontamination-treatments-an-in-vitro-study
#8
Cao Jie, Tong Wang, Pu Yinfei, Tang Zhihui, Meng Huanxin
OBJECTIVES: To investigate the effects of different decontamination treatments on microstructure of titanium (Ti) surface as well as proliferation and adhesion of human gingival fibroblasts (HGFs). MATERIAL AND METHODS: Ti discs with machined (M) and sand blasted, acid etched (SAE) surfaces were treated with five different decontamination treatments: (1) stainless steel curette (SSC), ultrasonic system with (2) straight carbon fiber tip (UCF) or (3) metal tip (UM), (4) rotating Ti brush (RTB), and (5) Er:YAG laser (30 mJ/pulse at 30 Hz)...
December 24, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29305174/inhibition-of-autophagy-reverses-alcohol-induced-hepatic-stellate-cells-activation-through-activation-of-nrf2-keap1-are-signaling-pathway
#9
Zheng-Yuan Xie, Zhi-Hua Xiao, Fen-Fen Wang
BACKGROUND: Numerous documents have indicated a critical role of autophagy in alcoholic liver fibrosis (ALF), but few papers have reported its function in hepatic stellate cells (HSCs) activation. The current study aimed to investigate the regulation effect of autophagy in HSCs activation, in further to explore the underlying mechanism involved. METHODS: HSC-T6 cells were treated with ethanol, 3-MA (autophagy inhibitor) or rapamycin (autophagy inducer), and cells were also transfected with si-Nrf2 or si-Keap1...
January 2, 2018: Biochimie
https://www.readbyqxmd.com/read/29285054/gene-expression-profiling-analysis-of-keloids-with-and-without-hydrocortisone-treatment
#10
Hongyi Wang, Liangliang Quan, Jiulong Liang, Jie Shi, Tao Qiu, Ye Zhang, Yang Wang, Qiang Hui, Yu Zhang, Kai Tao
The present study aimed to investigate the genetic effects of hydrocortisone (HC) treatment on keloids and screen medicines to be used in a combination therapy of keloids with HC. The dataset GSE7890 was downloaded from Gene Expression Omnibus. It contained data regarding 4 fibroblast samples from normal scar tissue and 5 samples from keloid tissue with HC treatment, as well as 5 samples from normal scar and 5 samples from keloids without HC treatment. Following the identification of differentially expressed genes (DEGs), the functions of these DEGs were analyzed by Gene Ontology (GO) and pathway enrichment analyses...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29281649/human-adipose-derived-stem-cells-regress-fibrosis-in-a-chronic-renal-fibrotic-model-induced-by-adenine
#11
Juan José Rivera-Valdés, Jesus García-Bañuelos, Adriana Salazar-Montes, Leonel García-Benavides, Alfredo Rosales-Dominguez, Juan Armendáriz-Borunda, Ana Sandoval-Rodríguez
BACKGROUND AND AIMS: ADSCs transplantation had been shown in some experimental models of kidney damage that it improves kidney function and reduces fibrosis. In this study we evaluated the effect of human adipose tissue-derived stem cell (hADSC) therapy in a chronic kidney damage experimental model. METHODS: A chronic kidney injury was induced by daily orogastric administration of adenine (100mg/kg) to male Wistar rats for 28 days. hADSCs were isolated, expanded and characterized before transplantation...
2017: PloS One
https://www.readbyqxmd.com/read/29281143/the-soluble-guanylate-cyclase-stimulator-iw-1973-prevents-inflammation-and-fibrosis-in-experimental-non-alcoholic-steatohepatitis
#12
Roger Flores-Costa, José Alcaraz-Quiles, Esther Titos, Cristina López-Vicario, Mireia Casulleras, Marta Duran-Güell, Bibiana Rius, Alba Diaz, Katherine Hall, Courtney Shea, Renee Sarno, Mark Currie, Jaime L Masferrer, Joan Clària
BACKGROUND AND PURPOSE: Non-alcoholic steatohepatitis (NASH) is the hepatic manifestation of the metabolic syndrome and is characterized by steatosis, inflammation and fibrosis. Soluble guanylate cyclase (sGC) stimulation reduces inflammation and fibrosis in experimental models of lung, kidney and heart disease. Here, we tested whether sGC stimulation is also effective in experimental NASH. EXPERIMENTAL APPROACH: Mice (n=35) were induced to NASH by a choline-deficient, L-amino acid-defined, high-fat (CDAAH) diet and received either placebo or the sGC stimulator IW-1973 at two different doses (1 and 3 mg/kg/day) for 9 weeks...
December 27, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29277342/do-immune-cells-lead-the-way-in-subchondral-bone-disturbance-in-osteoarthritis
#13
REVIEW
Adrian Weber, Pok Man Boris Chan, Chunyi Wen
Osteoarthritis (OA) is a whole-joint disorder, and non-cartilage articular pathologies, e.g. subchondral bone disturbance, contribute substantially to the onset and progression of the disease. In the early stage of OA, abnormal mechanical loading leads to micro-cracks or micro-fractures that trigger a reparative process with angiogenesis and inflammatory response. With the progression of disease, cystic lesion, sclerosis and osteophytosis occur at tissue level, and osteoblast dysfunction at cellular level. Osteoblasts derived from OA sclerotic bone produce increased amount of type I collagen with aberrant Col1A1/A2 ratio and poor mineralization capability...
December 22, 2017: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/29275289/role-of-fluoride-induced-histone-trimethylation-in-development-of-skeletal-fluorosis
#14
Atul P Daiwile, Saravanadevi Sivanesan, Prashant Tarale, Pravin K Naoghare, Amit Bafana, Devendra Parmar, Krishnamurthi Kannan
Chronic exposure to fluoride has been associated with the development of skeletal fluorosis. Limited reports are available on fluoride induced histone modification. However, the role of histone modification in the pathogenesis of skeletal fluorosis is not investigated. In the present study, we have investigated the role of fluoride induced histone modification on fluorosis development using human osteosarcoma (HOS) cell line. The expression of histone methyltransferases (EHMT1 and EHZ2) and level of global histone trimethylation (H3K9 and H3K27) have been assessed and observed to be increased significantly after fluoride exposure (8 mg/L)...
December 17, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29245016/preferential-myofibroblast-differentiation-of-cardiac-mesenchymal-progenitor-cells-in-the-presence-of-atrial-fibrillation
#15
Elisa Gambini, Gianluca Lorenzo Perrucci, Beatrice Bassetti, Gabriella Spaltro, Giulia Campostrini, Maria Chiara Lionetti, Alberto Pilozzi, Federico Martinelli, Andrea Faruggia, Dario DiFrancesco, Andrea Barbuti, Giulio Pompilio
Atrial fibrillation (AF) is characterized by electrical, contractile, and structural remodeling mediated by interstitial fibrosis. It has been shown that human cardiac mesenchymal progenitor cells (CMPCs) can be differentiated into endothelial, smooth muscle, and fibroblast cells. Here, we have investigated, for the first time, the contribution of CMPCs in the fibrotic process occurring in AF. As expected, right auricolae samples displayed significantly higher fibrosis in AF vs control (CTR) patients. In tissue samples of AF patients only, double staining for c-kit and the myofibroblast marker α-smooth muscle actin (α-SMA) was detected...
November 27, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/29240779/impact-of-maternal-high-fat-diet-on-hypothalamic-transcriptome-in-neonatal-sprague-dawley-rats
#16
Sanna Barrand, Tamsyn M Crowley, Ryan J Wood-Bradley, Kirstie A De Jong, James A Armitage
Maternal consumption of a high fat diet during early development has been shown to impact the formation of hypothalamic neurocircuitry, thereby contributing to imbalances in appetite and energy homeostasis and increasing the risk of obesity in subsequent generations. Early in postnatal life, the neuronal projections responsible for energy homeostasis develop in response to appetite-related peptides such as leptin. To date, no study characterises the genome-wide transcriptional changes that occur in response to exposure to high fat diet during this critical window...
2017: PloS One
https://www.readbyqxmd.com/read/29240584/dermatofibrosarcoma-protuberans-of-distal-extremities-and-acral-sites-a-clinicopathologic-analysis-of-27-cases
#17
Kabeer K Shah, Jonathan B McHugh, Andrew L Folpe, Rajiv M Patel
Dermatofibrosarcoma protuberans (DFSP) of the distal extremities and acral sites are extremely rare and incompletely characterized. Twenty-seven DFSP occurring in these sites were retrieved from our collective archives and reevaluated. Tumors occurred in 16 males and 11 females. Median age at presentation was 42.5 years (range, 7 to 78 y). Lesions involved the foot (18 with 6 in the toes and 2 on the plantar foot), distal ankle (4), hand (4 with 2 in the thumbs), and wrist (1). All cases showed predominantly classic DFSP morphology and were diffusely CD34 positive...
December 7, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29238307/in-vivo-evaluation-of-biocompatibility-and-chondrogenic-potential-of-a-cell-free-collagen-based-scaffold
#18
Giovanna Calabrese, Rosario Gulino, Raffaella Giuffrida, Stefano Forte, Elisa Figallo, Claudia Fabbi, Lucia Salvatorelli, Lorenzo Memeo, Massimo Gulisano, Rosalba Parenti
Injured articular cartilage has a limited innate regenerative capacity, due to the avascular nature and low cellularity of the tissue itself. Although several approaches have been proposed to repair the joint cartilage, none of them has proven to be effective. The absence of suitable therapeutic options has encouraged tissue-engineering approaches combining specific cell types and biomaterials. In the present work, we have evaluated the potential of a cell-free Collagen I-based scaffold to promote the augmentation of cartilage-like phenotype after subcutaneous implantation in the mouse...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29230389/cyclic-tensile-strain-can-play-a-role-in-directing-both-intramembranous-and-endochondral-ossification-of-mesenchymal-stem-cells
#19
Simon F Carroll, Conor T Buckley, Daniel J Kelly
Successfully regenerating damaged or diseased bone and other joint tissues will require a detailed understanding of how joint specific environmental cues regulate the fate of progenitor cells that are recruited or delivered to the site of injury. The goal of this study was to explore the role of cyclic tensile strain (CTS) in regulating the initiation of mesenchymal stem cell/multipotent stromal cell (MSC) differentiation, and specifically their progression along the endochondral pathway. To this end, we first explored the influence of CTS on the differentiation of MSCs in the absence of any specific growth factor, and secondly, we examined the influence of the long-term application of this mechanical stimulus on markers of endochondral ossification in MSCs maintained in chondrogenic culture conditions...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29228930/gene-expression-analysis-in-asthma-using-a-targeted-multiplex-array
#20
Christopher D Pascoe, Ma'en Obeidat, Bryna A Arsenault, Yunlong Nie, Stephanie Warner, Dorota Stefanowicz, Samuel J Wadsworth, Jeremy A Hirota, S Jasemine Yang, Delbert R Dorscheid, Chris Carlsten, Tillie L Hackett, Chun Y Seow, Peter D Paré
BACKGROUND: Gene expression changes in the structural cells of the airways are thought to play a role in the development of asthma and airway hyperresponsiveness. This includes changes to smooth muscle contractile machinery and epithelial barrier integrity genes. We used a targeted gene expression arrays to identify changes in the expression and co-expression of genes important in asthma pathology. METHODS: RNA was isolated from the airways of donor lungs from 12 patients with asthma (8 fatal) and 12 non-asthmatics controls and analyzed using a multiplexed, hypothesis-directed platform to detect differences in gene expression...
December 11, 2017: BMC Pulmonary Medicine
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