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https://www.readbyqxmd.com/read/28727318/appropriate-cyclic-tensile-strain-promotes-biological-changes-of-cranial-base-synchondrosis-chondrocytes
#1
F Chu, Q Feng, Z Hu, G Shen
OBJECTIVES: This study was designed to clarify biological changes of cranial base synchondrosis chondrocytes (CBSCs) upon cyclic tensile strain (CTS) loading which simulated orthopaedic mechanical protraction on cranial base synchondroses (CBS). MATERIAL AND METHODS: A two-step digestion method was used to isolate CBSCs obtained from 1-week-old Sprague Dawley rats. Immunohistochemical staining of type II collagen and Sox9 was conducted to identify chondrocytes. A CTS of 1 Hz and 10% elongation was applied to the second passage of CBSCs by FX-5000™ Tension System for 24 hours...
August 2017: Orthodontics & Craniofacial Research
https://www.readbyqxmd.com/read/28727179/overexpression-of-g%C3%AE-s-in-murine-osteoblasts-in-vivo-leads-to-increased-bone-mass-and-decreased-bone-quality
#2
Lucia Zhang, Kim S Sugamori, Colin Claridge, Ariana Dela Cruz, Marc D Grynpas, Jane Mitchell
GαS is a heterotrimeric G protein that transduces signals from activated G protein-coupled receptors on the cell surface to stimulate adenylyl cyclase/cyclic AMP signaling. GαS plays a central role in mediating numerous growth and maintenance processes including osteogenesis and bone turnover. Decreased GαS expression or activating mutations in GαS both affect bone suggesting that modulating GαS protein levels may be important for bone health and development. To examine the effects of increased osteoblastic GαS expression on bone development in vivo, we generated transgenic mice with GαS over-expression in osteoblasts (HOM-Gs mice) driven by the 3...
July 20, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28725987/gene-mutation-spectrum-and-genotype-phenotype-correlation-in-a-cohort-of-chinese-osteogenesis-imperfecta-patients-revealed-by-targeted-next-generation-sequencing
#3
Y Liu, Asan, D Ma, F Lv, X Xu, J Wang, W Xia, Y Jiang, O Wang, X Xing, W Yu, J Wang, J Sun, L Song, Y Zhu, H Yang, J Wang, M Li
The achievement of more accurate diagnosis would greatly benefit the management of patients with osteogenesis imperfecta (OI). In this study, we present the largest OI sample in China as screened by next generation sequencing. In particular, we successfully identified 81 variants, which included 45 novel variants. We further did a genotype-phenotype analysis, which helps make a better understanding of OI. INTRODUCTION: This study aims to reveal the gene mutation spectrum and the genotype-phenotype relationship among Chinese OI patients by next generation sequencing (NGS)...
July 19, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28716975/metabolic-phenotype-in-the-mouse-model-of-osteogenesis-imperfecta
#4
Iris Boraschi-Diaz, Josephine T Tauer, Omar El Rifai, Delphine Guillemette, Geneviève Lefebvre, Frank Rauch, Mathieu Ferron, Svetlana V Komarova
Osteogenesis Imperfecta (OI) is the most common heritable bone fragility disorder, usually caused by dominant mutations in genes coding for collagen type I alpha chains, COL1A1 or COL1A2. Osteocalcin is now recognized as a bone-derived regulator of insulin secretion and sensitivity and glucose homeostasis. Since OI is associated with increased rates of bone formation and resorption, we hypothesized that the levels of undercarboxylated osteocalcin are increased in OI. The objective of this study was to determine changes in osteocalcin and to elucidate the metabolic phenotype in the Col1a1Jrt/+ mouse, a model of dominant OI caused by a Col1a1 mutation...
July 17, 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28715254/effects-of-ethyl-%C3%AE-d-glucoside-on-human-dermal-fibroblasts
#5
Takayuki Bogaki, Keiichi Mitani, Yuki Oura, Kenji Ozeki
Ethyl α-d-glucoside (α-EG) is a glycoside present in sake, Japanese rice wine. Previous studies have reported that α-EG suppresses skin roughness after ultraviolet B irradiation, transepidermal water loss, and hepatic function disorder, and has a skin moisturizing effect. In this study, 0.48 μM of α-EG was found to increase the proliferation of normal human dermal fibroblasts (NHDF) by 121.0%, and the amount of collagen I produced by NHDF increased by 159.6% at an α-EG concentration of 0.048 μM, compared to those in cells cultured without α-EG...
July 17, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28713909/effects-of-lentiviral-vector%C3%A2-mediated-shrna-silencing-of-tgf%C3%AE-1-on-the-expression-of-col1a1-in-rat-hepatic-stellate-cells
#6
Gan-Ping Bai, Guo-He Yan, Guo-Jian Wang, Ping Wan, Xiao-Fei Liu, Heng Wang, Rong-Hua Zhang
The present study aimed to construct a lentiviral RNA interference (RNAi) vector targeting the transforming growth factor β1 (TGFβ1) gene of rats, in order to examine its effect on silencing of the TGFβ1 gene and on the expression of collagen type 1 α1 (Col1a1) in HSC‑T6 rat hepatic stellate cells. Three RNAi sites of the TGFβ1 gene were selected according to its CDs sequence. Three pairs of small interfering RNA (siRNA) of these RNAi sites were synthesized and then transfected into HSC‑T6 cells, respectively, to confirm the optimal siRNA sequence via reverse transcription‑polymerase chain reaction analysis...
July 6, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28708583/circular-rna-expression-profile-and-potential-function-of-hsa_circrna_101238-in-human-thoracic-aortic-dissection
#7
Meisheng Zou, Chixiong Huang, Xinzhong Li, Xiang He, Yanmei Chen, Wangjun Liao, Yulin Liao, Jie Sun, Ze Liu, Lintao Zhong, Jianping Bin
OBJECTIVE: To assess the circular RNAs (circRNAs) expression profile and explore the potential functions in human thoracic aortic dissection (TAD). METHODS: The differentially expressed circRNAs profiles of the aortic segments between human type A TAD patients (n=3) and age-matched normal donors (NA; n=3) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was used to validate the expression pattern of circRNAs, parental genes, and hsa-miR-320a; Western blotting confirmed MMP9 expression with additional samples...
July 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28704392/redifferentiation-of-aged-human-articular-chondrocytes-by-combining-bone-morphogenetic-protein-2-and-melanoma-inhibitory-activity-protein-in-3d-culture
#8
Stephan Payr, Brigitte Tichy, Clemens Atteneder, Marc Michel, Thomas Tiefenboeck, Nikolaus Lang, Sylvia Nuernberger, Stefan Hajdu, Elizabeth Rosado-Balmayor, Stefan Marlovits, Christian Albrecht
Melanoma inhibitory activity (MIA) affects the differentiation to hyaline cartilage and can inhibit the osteogenic potential of bone morphogenetic protein (BMP)-2 in mesenchymal stem cells (MSC). The aim of this study was to investigate if MIA also inhibits the osteogenic potential of BMP-2 in human articular chondrocytes during redifferentiation, which may lead to a higher grade of differentiation without calcification. HAC of four female patients (mean age: 73.75 ±6.98) were seeded into 3D culture for 28 days; after adding the recombinant proteins, four groups were formed (Control, BMP-2, MIA, BMP-2+MIA)...
2017: PloS One
https://www.readbyqxmd.com/read/28692738/inhibition-of-monocyte-chemoattractant-protein-1-prevents-conjunctival-fibrosis-in-an-experimental-model-of-glaucoma-filtration-surgery
#9
Rachel Shujuan Chong, Ying Shi Lee, Stephanie Wai Ling Chu, Li Zhen Toh, Tina Tzee Ling Wong
Purpose: To evaluate the effect of treatment with monocyte chemoattractant protein-1 receptor inhibitor (MCP-Ri) to maintain bleb survival and prevent fibrosis in an experimental model of glaucoma filtration surgery (GFS). Methods: GFS was performed on one eye of C57/Bl6 mice (n = 36) that was treated with MCP-Ri, mitomycin-C (MMC), or vehicle at the time of surgery. Real-time polymerase chain reaction was used to evaluate conjunctival expression of monocyte chemoattractant protein-1 (MCP-1), TGFB1, TGFB2, collagen 1a1 (Col1a1), sparc (Sparc), and fibronectin at 2 and 7 days following surgery...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28691479/effect-of-platelet-reach-plasma-on-fibroblast-primary-culture-gene-expression-evaluation
#10
V Candotto, A Palmieri, F Cura, F J Silvestre, D Lauritano, R M Gaudio, A Baj, A B Giannì
To correct skin imperfections, aesthetic medicine today makes use of injectable dermal fillers that allow filling skin by increasing the volume of soft tissue. Currently, there are many fillers usable for their excellent biocompatibility and low allergic reactions that ensure good results and good compliance. Among these, the most widely used filler is Human Plasma, obtained by centrifugation from patient blood and processed through cycles of centrifugation and heating, until it becomes a gel. In this work, we evaluated the effect of the Plasma on fibroblasts behavior in terms of cell viability, survival, growth and collagen production...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28691476/genetic-effects-of-vicryl%C3%A2-on-fibroblast-primary-culture
#11
A Baj, G A Beltramini, M Romano, D Lauritano, R M Gaudio, A Palmieri, F Cura, A B Giannì
Vicryl® (polyglactin 910) is an absorbable, synthetic, usually braided suture, indicated for soft tissue approximation and ligation. Vicryl® has a special coating for minimizing friction, easing passage through tissue and easy knot tie down. It is synthetic for minimal tissue reaction. Fibroblasts are the main cells of connective tissue that synthesize extracellular matrix. In this work, we tried to judge the action of Vicryl® on fibroblasts behaviour. We evaluated the expression levels of some adhesion and traction-resistance related genes (ELN, DSP, FN1, FBN1, ITGB1, ITGA1, ITGA5, ITGA2, COL1A1, COL3A1) by using real time Reverse Transcription-Polymerase Chain Reaction (real time RT-PCR)...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28691475/genetic-effects-of-biopad%C3%A2-on-fibroblast-primary-culture
#12
A Baj, G A Beltramini, M Romano, D Lauritano, R M Gaudio, A Palmieri, F Cura, A B Giannì
BIOPAD® is an ivory-white soft sponge, made exclusively of lyophilized type I native heterologous collagen extracted from horse flexor tendon, gelatine free, that keeps its native structure specific to the body’s skin tissue. BIOPAD® is an active dressing, playing an active role in all stages of wound healing process, stimulating granulation tissue growth and enhancing regeneration tissues. It ensures balance between absorption and humidity at wound surface, gaseous exchange of soft tissues during healing process, barrier to prevent bacterial infections and it is completely non-adherent...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28690010/tailoring-surface-nanoroughness-of-electrospun-scaffolds-for-skeletal-tissue-engineering
#13
Honglin Chen, Xiaobin Huang, Minmin Zhang, Febriyani Damanik, Matthew B Baker, Anne Leferink, Huipin Yuan, Roman Truckenmüller, Clemens van Blitterswijk, Lorenzo Moroni
Electrospun scaffolds provide a promising approach for tissue engineering as they mimic the physical properties of extracellular matrix. Previous studies have demonstrated that electrospun scaffolds with porous features on the surface of single fibers, enhanced cellular attachment and proliferation. Yet, little is known about the effect of such topographical cues on cellular differentiation. Here, we aimed at investigating the influence of surface roughness of electrospun scaffolds on skeletal differentiation of human mesenchymal stromal cells (hMSCs)...
July 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28689917/dioscin-a-potent-itga5-inhibitor-reduces-the-synthesis-of-collagen-against-liver-fibrosis-insights-from-silac-based-proteomics-analysis
#14
Lina Xu, Lianhong Yin, Xufeng Tao, Yan Qi, Xu Han, Youwei Xu, Shasha Song, Lei Li, Pengyuan Sun, Jinyong Peng
Inhibiting collagen generation is one effective method to treat liver fibrosis. Dioscin showed protective effect against liver fibrosis in our previous studies, and in the present work, SILAC-based proteomics was employed to test the underlying mechanism. A total of 121 differentially expressed proteins caused by dioscin in LX-2 cells were found, and dioscin significantly decreased the expression levels of FN, FAK1, ITGA5, p-PI3K/PI3K, p-Akt/Akt, p-mTOR/mTOR, Col1a1, Col1a2, Col2a1, Col5a1, Col6a1, and increased 2ABB level in vivo and in vitro...
July 6, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28679671/breast-regression-protein-39-chitinase-3-like-1-promotes-renal-fibrosis-after-kidney-injury-via-activation-of-myofibroblasts
#15
Tinika A Montgomery, Leyuan Xu, Sherene Mason, Amirtha Chinnadurai, Chun Geun Lee, Jack A Elias, Lloyd G Cantley
The normal response to kidney injury includes a robust inflammatory infiltrate of PMNs and macrophages. We previously showed that the small secreted protein breast regression protein-39 (BRP-39), also known as chitinase 3-like 1 (CHI3L1) and encoded by the Chi3l1 gene, is expressed at high levels by macrophages during the early stages of kidney repair and promotes tubular cell survival via IL-13 receptor α2 (IL13Rα2)-mediated signaling. Here, we investigated the role of BRP-39 in profibrotic responses after AKI...
July 5, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28669821/diet-enrichment-with-a-specific-essential-free-amino-acid-mixture-improves-healing-of-undressed-wounds-in-aged-rats
#16
Giovanni Corsetti, Claudia Romano, Evasio Pasini, Emanuele Marzetti, Riccardo Calvani, Anna Picca, Vincenzo Flati, Francesco S Dioguardi
Chronic wounds are a major, often underestimated, health problem for the elderly. Standard wound care products are not usually manufactured to meet the increased demand of nutrients by skin cells in order to regenerate new tissue and accelerate healing. This work was therefore undertaken to establish whether wound healing could be accelerated by nutritional supplementation with a specific mixture tailored to human need of essential amino acids (EAAs) without topical medication. To this end, using a skin full-thickness excisional model in aged rats, we compared the closure dynamics of undressing wounds in animals fed an EAAs-enriched diet or standard diet...
June 29, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28668298/interstitial-fluid-flow-induced-growth-potential-and-hyaluronan-synthesis-of-fibroblasts-in-a-fibroblast-populated-stretched-collagen-gel-culture
#17
Natsumi Saito, Hiroaki Adachi, Hiroshi Tanaka, Satoru Nakata, Norifumi Kawada, Ken Oofusa, Katsutoshi Yoshizato
BACKGROUND: Tensioned collagen gels with dermal fibroblasts (DFs) as a dermis model are usually utilized in a static culture (SC) that lacks medium flowing. To make the model closer to its in vivo state, we created a device to perfuse the model with media flowing at a physiological velocity and examined the effects of medium flow (MF) on the cultures. METHODS: We constructed a medium perfusion device for human DF-embedded stretched collagen gels (human dermis model), exposed the model to media that flows upwardly at ~1mL/day, and examined water retention of the gels, cells' growth ability, metabolic activity, expression profiles of nine extracellular matrix (ECM)-related genes...
June 28, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28668235/severe-hypotonia-and-postnatal-growth-impairment-in-a-girl-with-a-missense-mutation-in-col1a1-implication-of-expanded-phenotypic-spectrum-of-type-i-collagenopathy
#18
Jin Sook Lee, Jieun Seo, Anna Cho, Byung Chan Lim, Murim Choi, Jung-Wook Kim, Ok-Hwa Kim, Tae-Joon Cho, Jong-Hee Chae
BACKGROUND: It is known that type I collagenopathy has a broad-spectrum phenotypic variability. Here, we report a case of a Korean girl with a heterozygous COL1A1 mutation who had an atypical presentation. CASE PRESENTATION: A 26-month-old girl presented with delayed motor development and failure to thrive. She had severe growth retardation. She exhibited right-sided plagiocephaly, blue sclerae, and facial dysmorphism, including a small pointed chin, frontal bossing, and a triangular face, but had microcephaly...
June 28, 2017: Brain & Development
https://www.readbyqxmd.com/read/28665926/exome-sequencing-revealed-a-novel-homozygous-splice-site-variant-in-wnt1-gene-underlying-osteogenesis-imperfecta-type-3
#19
Muhammad Umair, Bader Alhaddad, Afzal Rafique, Abid Jan, Tobias B Haack, Elisabeth Graf, Asmat Ullah, Farooq Ahmad, Tim M Strom, Thomas Meitinger, Wasim Ahmad
BACKGROUND: Osteogenesis Imperfecta (OI) is a heritable bone fragility disorder usually caused by dominant variants in COL1A1 or COL1A2 genes. Over the last few years, seventeen genes including twelve autosomal recessive and five autosomal dominant forms of OI, involved in various aspects of bone formation, have been identified. METHODS: Whole exome sequencing followed by conventional Sanger sequencing was performed in single affected individual IV-3 in the present family...
June 30, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28657647/influences-of-pon1-on-airway-inflammation-and-remodeling-in-bronchial-asthma
#20
Wei-Qiang Chen, Zuo-Zhou Xie, Xiang Wang, Jin-Hong Zhao, Qin Hu, Ying-Hua Chen, Wen-Yong Gao, Yi Liu
This study aims to explore the influences of Paraoxonase-1 (PON1) involved in airway inflammation and remodeling in asthma. Mice were divided into control, asthma, asthma + PON1 and asthma + NC groups, and asthma models were established via aerosol inhalation of ovalbumin (OVA). HE, Masson and PAS stains were used to observe airway inflammation and remodeling, Giemsa staining to assess inflammatory cells in bronchoalveolar lavage fluid (BALF), qRT-PCR and western blot to detect PON1 expression, lipid peroxidation and glutathione assays to quantify malondialdehyde (MDA) activity and glutathione peroxidase (GSH) levels, ELISA to determine inflammatory cytokines and immunoglobulin, and colorimetry to detect PON1 activities...
June 28, 2017: Journal of Cellular Biochemistry
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