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https://www.readbyqxmd.com/read/29224239/personalized-stepwise-vascular-control-during-complex-hepatectomy-involving-hepatocaval-confluence
#1
Lei Dou, Zong-Ping Yu, Hui-Yuan Yang, Ji Ran, Yi-Fa Chen, Xiao-Ping Chen
BACKGROUND: This study introduces an innovative stepwise vascular control technique to address the high risk of massive bleeding from main hepatic veins and the retro-hepatic inferior vena cava during hepatectomy involving hepatocaval confluence. METHODS: From January 2010 to July 2016, 80 patients underwent stepwise vascular occlusion during complex liver resection involving hepatocaval confluence. Relevant clinical data were collected and compared with those obtained in parallel studies...
December 10, 2017: ANZ Journal of Surgery
https://www.readbyqxmd.com/read/29224229/power-doppler-ultrasound-assessment-of-vascularization-in-hidradenitis-suppurativa-lesions
#2
Raffaele Dante Caposiena Caro, Francesco Maria Solivetti, Luca Bianchi
BACKGROUND: Ultrasound and Power Doppler (PD) ultrasound are useful tools to study and monitor Hidradenitis Suppurativa (HS) patients. OBJECTIVE: Describe the Power Doppler signal of HS nodules, abscesses, fistulas. METHODS: A retrospective analysis of PD in mild, moderate, severe HS patients, collecting all demographic and clinical data. The lesions were classified according to their ultrasound morphology, describing the vascular degree - high, moderate, minimal - and distribution - peripheral, internal, mixed...
December 10, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29224223/altering-the-school-breakfast-environment-reduces-barriers-to-school-breakfast-participation-among-diverse-rural-youth
#3
Mary O Hearst, Amy Shanafelt, Qi Wang, Robert Leduc, Marilyn S Nanney
BACKGROUND: The purpose is to determine if altering school breakfast policies and the school breakfast environment will positively impact adolescent beliefs of the barriers and benefits of eating breakfast. METHODS: There were 904 adolescents from 16 rural high schools, Minnesota, in the BreakFAST Study who reported eating breakfast fewer than 4 times per week at baseline. Schools were randomized to intervention (N = 8 schools) or delayed intervention (N = 8) condition...
January 2018: Journal of School Health
https://www.readbyqxmd.com/read/29224216/association-of-school-based-physical-activity-opportunities-socioeconomic-status-and-third-grade-reading
#4
Ben D Kern, Kim C Graber, Sa Shen, Charles H Hillman, Gabriella McLoughlin
BACKGROUND: Socioeconomic status (SES) is the most accurate predictor of academic performance in US schools. Third-grade reading is highly predictive of high school graduation. Chronic physical activity (PA) is shown to improve cognition and academic performance. We hypothesized that school-based PA opportunities (recess and physical education) would moderate the negative association between SES and third-grade reading. METHODS: Schools serving third-grade students were surveyed (N = 1279) for minutes/week of PA opportunities...
January 2018: Journal of School Health
https://www.readbyqxmd.com/read/29224187/surma-eye-cosmetic-in-afghanistan-a-potential-source-of-lead-toxicity-in-children
#5
J R McMichael, B K Stoff
Surma is a traditional eye cosmetic used as an eyeliner for infants in Afghanistan, as well as in many other countries in Asia, the Middle East, and Africa. Surma has been reported to contain lead and to be a potential source of lead toxicity in children, which can lead to permanent damage to multiple organ systems. To our knowledge, assessment for lead in surma found in Afghanistan has not been performed. We determined the quantitative lead content of a convenience sample of 10 surma products acquired in Afghanistan...
December 9, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/29224181/social-cognition-and-aggression-in-methamphetamine-dependence-with-and-without-a-history-of-psychosis
#6
Anne Uhlmann, Jonathan C Ipser, Don Wilson, Dan J Stein
In substance use and psychotic disorders, socially problematic behaviours, such as high aggression may, in part, be explained by deficits in social cognition skills, like the detection of emotions or intentions in others. The aim of this study was to assess the magnitude of social cognition impairment and its association with aggression in individuals with methamphetamine (MA) dependence, methamphetamine-associated psychosis (MAP), and healthy controls (CTRL). A total of 20 MAP participants, 21 MA-dependent participants without psychosis, and 21 CTRL participants performed a facial morphing emotion recognition task (ERT) across four basic emotions (anger, fear, happiness and sadness) and the reading the mind in the eyes task (RMET), and completed the aggression questionnaire...
December 9, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/29224163/methylation-sensitive-high-resolution-melting-ms-hrm
#7
Dianna Hussmann, Lise Lotte Hansen
Methylation-Sensitive High Resolution Melting (MS-HRM) is an in-tube, PCR-based method to detect methylation levels at specific loci of interest. A unique primer design facilitates a high sensitivity of the assays enabling detection of down to 0.1-1% methylated alleles in an unmethylated background.Primers for MS-HRM assays are designed to be complementary to the methylated allele, and a specific annealing temperature enables these primers to anneal both to the methylated and the unmethylated alleles thereby increasing the sensitivity of the assays...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224161/quantitative-region-specific-dna-methylation-analysis-by-the-epityper%C3%A2-technology
#8
Sonja Kunze
DNA methylation plays a profound role in development and health as well as development and progression of disease. High-throughput quantitative DNA methylation analysis is therefore crucial for the study of the normal physiology of the epigenome and its dysregulation in disease. Many target areas are identified by a range of emerging genome-wide cytosine methylation techniques, but these whole genome scans usually only provide methylation data for a few individual CpG sites (CpGs) within a region. The EpiTYPER™ assay is a region-specific method for the detection and quantitative analysis of DNA methylation that allows performing a high-resolution scan of selected regions...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224160/methylight-and-digital-methylight
#9
Mihaela Campan, Daniel J Weisenberger, Binh Trinh, Peter W Laird
MethyLight is a quantitative, fluorescence-based, real-time PCR method to sensitively detect and quantify DNA methylation of candidate regions of the genome. MethyLight is uniquely suited for detecting low-frequency methylated DNA regions against a high background of unmethylated DNA, as it combines methylation-specific priming with methylation-specific fluorescent probing. The quantitative accuracy of real-time PCR and the ability to design bisulfite-dependent, DNA methylation-independent control reactions together allow for a quantitative assessment of these low frequency methylation events...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224156/multiplexed-and-sensitive-dna-methylation-testing-using-methylation-sensitive-restriction-enzymes-msre-qpcr
#10
Gabriel Beikircher, Walter Pulverer, Manuela Hofner, Christa Noehammer, Andreas Weinhaeusel
DNA methylation is a chemically stable key-player in epigenetics. In the vertebrate genome the 5-methyl cytosine (5mC) has been found almost exclusively in the CpG dinucleotide context. CpG dinucleotides are enriched in CpG islands very frequently located within or close to gene promoters. Analyses of DNA methylation changes in human diagnostics have been conducted classically using methylation-sensitive restriction enzymes (MSRE). Since the discovery of bisulfite conversion-based sequencing and PCR assays, MSRE-based PCR assays have been less frequently used, although especially in the field of cancer epigenetics MSRE-based genome-wide discovery and targeted screening applications have been and are still performed successfully...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224153/multiplexed-dna-methylation-analysis-of-target-regions-using-microfluidics-fluidigm
#11
Martyna Adamowicz, Klio Maratou, Timothy J Aitman
Whole genome shotgun bisulfite sequencing is a method used to generate genome-wide methylation profiles. There are many available protocols to validate the results of this genome-wide method, but they mostly share the limitation of measuring methylation at a small number of CpG positions in small numbers of samples. We developed a multiplexed DNA methylation analysis protocol, which allows for the simultaneous quantitative measurement of cytosine methylation at single nucleotide resolution in 48 PCR amplicons and 48 samples utilizing the microfluidic system established by Fluidigm...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224144/low-input-whole-genome-bisulfite-sequencing-using-a-post-bisulfite-adapter-tagging-approach
#12
Julian R Peat, Sébastien A Smallwood
The epigenetic mark 5-methylcytosine confers heritable regulation of gene expression that can be dynamically modulated during transitions in cell fate. With the development of high-throughput sequencing technologies, it is now possible to obtain comprehensive genome-wide maps of the mammalian DNA methylation landscape, but the application of these techniques to limited material remains challenging. Here, we present an optimized protocol to perform whole-genome bisulfite sequencing on low inputs (100-5000 somatic cells) using a post-bisulfite adapter tagging approach...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224138/quantification-of-global-dna-methylation-levels-by-mass-spectrometry
#13
Agustin F Fernandez, Luis Valledor, Fernando Vallejo, Maria Jesús Cañal, Mario F Fraga
Global DNA methylation was classically considered the relative percentage of 5-methylcysine (5mC) with respect to total cytosine (C). Early approaches were based on the use of high-performance separation technologies and UV detection. However, the recent development of protocols using mass spectrometry for the detection has increased sensibility and permitted the precise identification of peak compounds based on their molecular masses. This allows work to be conducted with much less genomic DNA starting material and also to quantify 5-hydroxymethyl-cytosine (5hmC), a recently identified form of methylated cytosine that could play an important role in active DNA demethylation...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224130/molecular-epidemiology-and-characterization-of-bovine-leukemia-virus-in-domestic-yaks-bos-grunniens-on-the-qinghai-tibet-plateau-china
#14
Meng Wang, Yun Wang, Abdul Rasheed Baloch, Yangyang Pan, Fang Xu, Lili Tian, Qiaoying Zeng
Bovine leukemia virus (BLV) is a member of the genus Deltaretrovirus of the family Retroviridae and cause a chronic lymphosarcoma, which is extensive in cattle. In yaks (Bos grunniens), the distribution, strains and genetic characteristics of BLV have rarely been studied. The aim of our study was to investigate BLV infections in domestic yaks and determine the genetic variability of BLV circulating in a region of the Qinghai Tibet Plateau, China. Blood samples were collected from 798 yaks, which were from different farms from Gansu, Qinghai and Sichuan provinces surrounding the Qinghai-Tibet Plateau...
December 9, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29224072/snp-discovery-from-single-and-multiplex-genome-assemblies-of-non-model-organisms
#15
Phillip A Morin, Andrew D Foote, Christopher M Hill, Benoit Simon-Bouhet, Aimee R Lang, Marie Louis
Population genetic studies of non-model organisms often rely on initial ascertainment of genetic markers from a single individual or a small pool of individuals. This initial screening has been a significant barrier to beginning population studies on non-model organisms (Aitken et al., Mol Ecol 13:1423-1431, 2004; Morin et al., Trends Ecol Evol 19:208-216, 2004). As genomic data become increasingly available for non-model species, SNP ascertainment from across the genome can be performed directly from published genome contigs and short-read archive data...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224070/genome-wide-analysis-of-dna-methylation-in-single-cells-using-a-post-bisulfite-adapter-tagging-approach
#16
Heather J Lee, Sébastien A Smallwood
DNA methylation is an epigenetic mark implicated in the regulation of key biological processes. Using high-throughput sequencing technologies and bisulfite-based approaches, it is possible to obtain comprehensive genome-wide maps of the mammalian DNA methylation landscape with a single-nucleotide resolution and absolute quantification. However, these methods were only applicable to bulk populations of cells. Here, we present a protocol to perform whole-genome bisulfite sequencing on single cells (scBS-Seq) using a post-bisulfite adapter tagging approach...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224063/an-efficient-scheme-for-mental-task-classification-utilizing-reflection-coefficients-obtained-from-autocorrelation-function-of-eeg-signal
#17
M M Rahman, M A Chowdhury, S A Fattah
Classification of different mental tasks using electroencephalogram (EEG) signal plays an imperative part in various brain-computer interface (BCI) applications. In the design of BCI systems, features extracted from lower frequency bands of scalp-recorded EEG signals are generally considered to classify mental tasks and higher frequency bands are mostly ignored as noise. However, in this paper, it is demonstrated that high frequency components of EEG signal can provide accommodating data for enhancing the classification performance of the mental task-based BCI...
December 9, 2017: Brain Informatics
https://www.readbyqxmd.com/read/29224061/cyclic-fatigue-and-torsional-strength-of-three-different-thermally-treated-reciprocating-nickel-titanium-instruments
#18
Murilo Priori Alcalde, Marco Antonio Hungaro Duarte, Clovis Monteiro Bramante, Bruno Carvalho de Vasconselos, Mario Tanomaru-Filho, Juliane Maria Guerreiro-Tanomaru, Jader Carvalho Pinto, Marcus Vinicius Reis Só, Rodrigo Ricci Vivan
OBJECTIVES: The aim of this study was to evaluate the cyclic and torsional fatigue resistance of the reciprocating single-file systems Reciproc Blue 25.08 (VDW GmbH, Munich, Germany), Prodesign R 25.06 (Easy Dental Equipment, Belo Horizonte, Brazil), and WaveOne Gold 25.07 (Dentsply/Tulsa Dental Specialties, Tulsa, OK, USA). MATERIALS AND METHODS: Sixty reciprocating instruments of the systems Reciproc Blue R25 (RB #25 .08 taper), Prodesign R (PDR #25 .06 taper), and WaveOne Gold (WOG #25 ...
December 9, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29224059/lower-complication-rate-and-faster-return-to-sports-in-patients-with-acute-syndesmotic-rupture-treated-with-a-new-knotless-suture-button-device
#19
Christian Colcuc, Marc Blank, Thomas Stein, Florian Raimann, Sanjay Weber-Spickschen, Sebastian Fischer, Reinhard Hoffmann
PURPOSE: Suture button devices for tibiofibular syndesmosis injuries provide semirigid dynamic stabilization, but complications including knot irritation have been reported. No randomized trials of the new knotless suture button devices have been performed. We hypothesized that knotless suture button devices eliminate knot irritation and facilitate quicker return to sports. This study was performed to compare the clinical outcomes, complication rates, and time to return to sports between a new knotless suture button device and syndesmotic screw fixation...
December 9, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/29224057/identifying-factors-associated-with-need-for-flexible-ureteroscope-repair-a-western-endourology-stone-west-research-consortium-prospective-cohort-study
#20
Kazumi Taguchi, Jonathan D Harper, Marshall L Stoller, Brian D Duty, Mathew D Sorensen, Roger L Sur, Manint Usawachintachit, David T Tzou, David L Wenzler, Dylan Isaacson, Angela Xu, Carissa Chu, Uwais B Zaid, Eric R Taylor, Krishna Ramaswamy, Thomas Chi
Maintenance of flexible ureteroscopes can involve high costs and administrative burden. Instrument fragility necessitates eventual repair, rendering scopes inaccessible during refurbishment. We conducted a multi-institutional prospective cohort study to identify perioperative factors influencing flexible ureteroscope durability. Patients undergoing flexible ureteroscopy (URS) at six United States endourology centers were enrolled between August 2014 and June 2015. Surgeon self-reported concern and satisfaction with scope performance as well as upward and downward angles of deflection for each scope tip were measured before and after each procedure...
December 9, 2017: Urolithiasis
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