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https://www.readbyqxmd.com/read/28445960/effect-of-folate-deficiency-on-promoter-methylation-and-gene-expression-of-esr1-cav1-and-elavl1-and-its-influence-on-spermatogenesis
#1
Hong-Fang Yuan, Kai Zhao, Yu Zang, Chun-Yan Liu, Zhi-Yong Hu, Jia-Jing Wei, Ting Zhou, Ying Li, Hui-Ping Zhang
This study aims to investigate the effect of folate deficiency on the male reproductive function and the underlying mechanism. A total of 269 screened participants from 421 recruitments were enrolled in this study. An animal model of folate deficiency was constructed. Folate concentration was measured in the ejaculate, and its association with semen parameters was then determined. The expression and promoter methylation status of ESR1, CAV1, and ELAVL1 were also evaluated. Results showed that seminal plasma folate level was significantly lower among subjects with azoospermia than those with normozoospermia...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28443587/role-of-trace-elements-for-oxidative-status-and-quality-of-human-sperm
#2
Galina Nenkova, Lubomir Petrov, Albena Alexandrova
BACKGROUND: Oxidative stress affects negatively the sperm quality. For maintaining the pro/antioxidative balance, some metal ions (e.g. copper, zinc, iron, selenium), which are co-factors of the antioxidant enzymes, are essential. However, iron and copper could act as prooxidants inducing oxidative damage of spermatozoa. AIMS: This study aims to reveal a possible correlation between the concentrations of some metal ions (iron, copper, zinc, and selenium) in human seminal plasma, oxidative stress, assessed by malondialdehyde (MDA) and total glutathione (tGSH) levels, and semen quality, assessed by the parameters count, motility, and morphology...
April 6, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28443258/the-effect-of-first-nocturnal-ejaculation-timing-on-risk-and-sexual-behaviors-of-korean-male-adolescents
#3
Mi-Ji Lee, Go-Eun Yang, Hee Won Chueh, Jae Hong Park, Jae-Ho Yoo
PURPOSE: This study evaluated the effect of first nocturnal ejaculation timing on risk and sexual behaviors of Korean male adolescents. METHODS: We analyzed data from the 10th edition of the Korea Youth Risk Behavior Web-based survey that was conducted with male high school adolescents in grades 10-12. The survey included 17,907 adolescents, and 10,326 responded their experience of first nocturnal ejaculation. Of these, 595 had their first nocturnal ejaculation in ≤grade 4 ("early puberty") and 9,731 had their first nocturnal ejaculation in ≥grade 5 ("normal puberty")...
March 2017: Annals of Pediatric Endocrinology & Metabolism
https://www.readbyqxmd.com/read/28442107/in%C3%A2-vitro-and-in%C3%A2-vivo-investigation-of-natural-compounds-from-seed-extract-of-mucuna-pruriens-lacking-l-dopa-for-the-treatment-of-erectile-dysfunction
#4
Natthachai Duangnin, Thanyaluck Phitak, Peraphan Pothacharoen, Prachya Kongtawelert
OBJECTIVE: To investigate the biological effects of the Mucuna pruriens (M. pruriens) seed extracts that lacked l-DOPA, which was formerly reported as the active ingredient, on erectile dysfunction (ED) both in vitro and in vivo. METHODS: Seed of M. pruriens plant that cultivated in Mae Taeng District, Chiang Mai Province, Thailand, was collected. Component of its seeds were extracted and isolated into 2 fractions using methanol, polar and nonpolar. Each fraction was investigated for phytochemicals using gas chromatography and mass spectroscopy and was screened for biological activity in vitro using three different cell lines...
March 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28442061/effect-of-docosahexanoic-acid-on-quality-of-frozen-thawed-bull-semen-in-bioxcell-extender
#5
Asmatullah Kaka, Wahid Haron, Rosnina Yusoff, Nurhusien Yimer, A M Khumran, Kazhal Sarsaifi, Atique Ahmed Behan, Ubedullah Kaka, Akeel Ahmed Memon, Mahdi Ebrahimi
This study was conducted to investigate the effect of docosahexanoic acid (DHA) supplementation in BioXcell extender on the quality of frozen-thawed bull semen. Twenty-four ejaculates were collected from three bulls (eight from each bull). Ejaculates with motility ≥70% and normal morphology ≥80% were extended into BioXcell extender to which 0 (control), 3, 5, 10 or 15ngmL(-1) DHA was added. The supplemented semen samples were incubated at 37°C for 15min for DHA uptake by spermatozoa. Later, samples were cooled for 2h at 5°C and packaged into 0...
March 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28442049/prion-protein-2-dublet-gene-prnd-role-in-ovine-semen-capacitation-cryopreservation-and-fertility
#6
L M Ferreira, M Garcia-Herreros, A Domingos, C C Marques, P Mesquita, J P Barbas, M C Baptista, J Pimenta, A E M Horta, J A M Prates, R M L N Pereira
The aim of the present study was to examine the role of Doppel protein in the capacitation process and fertilising ability of both fresh and frozen-thawed (FT) spermatozoa from rams carrying different prion protein 2 (dublet) (PRND) gene polymorphisms. The detection efficacy of new anti-Doppel monoclonal antibodies and PRND mRNA quantification were also explored in ovine spermatozoa. Three different genotypes (AA, GA, GG) were identified for codon 26 of ovine PRND-c.78G>A. Using flow cytometry, a higher fluorescence was detected in fresh compared with FT sperm samples incubated with anti-Doppel primary and fluorescein isothiocyanate-conjugated secondary antibodies (P<0...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28439908/possible-role-of-serum-testosterone-gonadotropins-and-prolactin-in-patients-with-premature-ejaculation
#7
M Abu El-Hamd, A Farah
Premature ejaculation (PE) is the most common male sexual dysfunction. This study aimed to investigate the role of serum testosterone, gonadotropins and prolactin in patients with PE. In a prospective a case-controlled study, it was conducted on 90 male patients with PE and 90 male healthy participants as controls. Patients were evaluated by Premature Ejaculation Diagnostic Tool (PEDT) and intravaginal ejaculatory latency time (IELT). Patients with mean IELT values ≤60 s and PEDT total scores ≥11 were considered to have PE...
April 24, 2017: Andrologia
https://www.readbyqxmd.com/read/28436120/efficacy-of-holmium-laser-transurethral-incision-of-the-prostate-in-symptomatic-mild-to-moderate-benign-prostate-enlargement-based-on-preoperative-characteristics
#8
Yoon Seok Suh, Kwang Jin Ko, Tae Heon Kim, Hyun Hwan Sung, Kyu-Sung Lee
OBJECTIVE: To investigate the efficacy of Holmium laser transurethral incision of the prostate (Ho-TUIP) with preoperative characteristics based on urodynamic parameters. METHODS: The medical records of 40 consecutive cases of Ho-TUIP in patients unresponsive to medical treatment were retrospectively reviewed. The efficacy of Ho-TUIP was analyzed according to preoperative factors, including urodynamic parameters. Treatment success was confirmed if overall efficacy demonstrated an improvement that was "good or greater" according to the criteria developed by Homma et al...
April 23, 2017: Lower Urinary Tract Symptoms
https://www.readbyqxmd.com/read/28434751/genome-wide-mapping-and-estimation-of-inbreeding-depression-of-semen-quality-traits-in-a-cattle-population
#9
Maja Ferenčaković, Johann Sölkner, Miroslav Kapš, Ino Curik
Inbreeding depression is known to affect quantitative traits such as male fertility and sperm quality, but the genetic basis for these associations is poorly understood. Most studies have been limited to examining how pedigree- or marker-derived genome-wide autozygosity is associated with quantitative phenotypes. In this study, we analyzed possible associations of genetic features of inbreeding depression with percentage of live spermatozoa and total number of spermatozoa in 19,720 ejaculates obtained from 554 Austrian Fleckvieh bulls during routine artificial insemination programs...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28431025/1h-magnetic-resonance-spectroscopy-of-live-human-sperm
#10
S Reynolds, S J Calvert, M N Paley, A A Pacey
STUDY QUESTION: Can 1H Magnetic Resonance Spectroscopy (MRS) be used to obtain information about the molecules and metabolites in live human spermatozoa? SUMMARY ANSWER: Percoll-based density gradient centrifugation (DGC) followed by a further two washing steps, yielded enough sperm with minimal contamination (<0.01%) from seminal fluid to permit effective MRS which detected significant differences (p < 0.05) in the choline/glycerophosphocholine, lipid and lactate regions of the 1H MRS spectrum between sperm in the pellet and those from the 40/80% interface...
April 18, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28429281/impact-of-thyroid-disease-on-testicular-function
#11
REVIEW
Sandro La Vignera, Roberto Vita, Rosita A Condorelli, Laura M Mongioì, Silvia Presti, Salvatore Benvenga, Aldo E Calogero
INTRODUCTION: Testis was considered unresponsive to thyroid hormone for a long time. However, like in animals, the presence of thyroid hormone receptors in different testicular cell types was demonstrated also in humans. Accordingly, thyrotoxicosis and hypothyroidism have remarkable effects on testicular function and more extensively on fertility. REVIEW: Thyrotoxicosis and hypothyroidism are associated with changes affecting the endocrine, sexual, or reproductive functions...
April 20, 2017: Endocrine
https://www.readbyqxmd.com/read/28428330/genomic-responses-to-socio-sexual-environment-in-male-drosophila-melanogaster-exposed-to-conspecific-rivals
#12
Irina I Mohorianu, Amanda Bretman, Damian T Smith, Emily Fowler, Tamas Dalmay, Tracey Chapman
Socio-sexual environments have profound effects on fitness. Local sex ratios can alter the threat of sexual competition, to which males respond via plasticity in reproductive behaviours and ejaculate composition. In Drosophila melanogaster, males detect the presence of conspecific mating rivals prior to mating using multiple, redundant sensory cues. Males that respond to rivals gain significant fitness benefits by altering mating duration and ejaculate composition. Here we investigated the underlying genome-wide changes involved...
April 20, 2017: RNA
https://www.readbyqxmd.com/read/28425158/sexual-colouration-and-sperm-performance-in-the-australian-painted-dragon-lizard-ctenophorus-pictus
#13
Callum S McDiarmid, Christopher R Friesen, Cissy Ballen, Mats Olsson
Theory predicts trade-offs between pre- and postcopulatory sexually selected traits. This relationship may be mediated by the degree to which males are able to monopolise access to females, as this will place an upper limit on the strength of postcopulatory selection. Furthermore, traits that aid in mate monopolization may be costly to maintain and may limit investment in postcopulatory traits, such as sperm performance. Australian painted dragons are polymorphic for the presence or absence of a yellow gular patch ('bibs'), which may aid them to monopolise access to females...
April 20, 2017: Journal of Evolutionary Biology
https://www.readbyqxmd.com/read/28418600/validation-of-a-laboratory-developed-test-of-human-sperm-capacitation
#14
Melissa A Moody, Cristina Cardona, Alana J Simpson, T Timothy Smith, Alexander J Travis, G Charles Ostermeier
Sperm must undergo capacitation to become fertilization competent. Here we validated that monosialotetrahexosylganglioside (GM1 ) localization patterns, which were assessed in the Cap-Score™ Sperm Function Test, reflect a capacitated state in human sperm. First, we defined patterns representing sperm that do or do not respond to stimuli for capacitation. Sperm with "capacitated" patterns had exposed acrosomal carbohydrates and underwent acrosome exocytosis in response to calcium ionophore (A23187). Precision was evaluated by percent change of the Cap-Score measured for 50, 100, 150, and 200 sperm...
April 18, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28417005/reversibility-of-vasalgel%C3%A2-male-contraceptive-in-a-rabbit-model
#15
Donald Waller, David Bolick, Elaine Lissner, Christopher Premanandan, Gary Gamerman
BACKGROUND: Development of a non-hormonal long-acting reversible contraceptive for men could have a significant impact on reducing unintended pregnancies. Vasalgel™ is a high molecular weight polymer consisting of styrene-alt-maleic acid (SMA) dissolved in dimethyl sulfoxide being developed as a reversible male contraceptive device. It forms a hydrogel when implanted into the vasa deferentia, which prevents the passage of sperm. Previous studies in the rabbit have proven its efficacy, durability and rapid onset...
2017: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/28414719/crucial-role-of-estrogen-for-the-mammalian-female-in-regulating-semen-coagulation-and-liquefaction-in-vivo
#16
Shuai Li, Marleny Garcia, Rachel L Gewiss, Wipawee Winuthayanon
Semen liquefaction changes semen from a gel-like to watery consistency and is required for sperm to gain mobility and swim to the fertilization site in the Fallopian tubes. Kallikrein-related peptidases 3 (KLK3) and other kallikrein-related peptidases from male prostate glands are responsible for semen liquefaction by cleaving gel-forming proteins (semenogelin and collagen). In a physiological context, the liquefaction process occurs within the female reproductive tract. How seminal proteins interact with the female reproductive environment is still largely unexplored...
April 17, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28414175/success-in-the-acquisition-of-bombyx-mori-sperm-motility-is-influenced-by-the-extracellular-production-of-nitric-oxide-no-in-the-presence-of-seminal-fluid-nitric-oxide-synthase-nos
#17
Sumiharu Nagaoka, Maiko Asagoshi, Keita Kato, Yuki Takata
A trypsin-like protease called initiatorin is known to initiate sperm motility in the silkworm, Bombyx mori, but little is known about the signaling events leading to sperm flagellar beating. The aim of this study was to investigate whether this mechanism of sperm motility activation involves the signaling transmitter nitric oxide (NO). NO is produced from the amino acid L-arginine by the enzyme action of nitric oxide synthase (NOS; EC 1.14.13.39). Simple treatment of quiescent sperm with an NO donor (SNAP or NOC7) in vitro did not lead to activation of motility...
April 13, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28414060/dietary-protein-content-alters-both-male-and-female-contributions-to-drosophila-melanogaster-female-post-mating-response-traits
#18
Janna N Schultzhaus, Ginger E Carney
Males transfer sperm, proteins and other molecules to females during mating. In Drosophila melanogaster, these molecules contribute to the induction of egg maturation, ovulation, oviposition, sperm storage and changes in female receptivity. This suite of physiological and behavioral changes is referred to as the female post-mating response (PMR). Protein is a necessary macronutrient for both male and female reproduction, but imbalances in protein content can decrease reproductive potential. Dietary protein affects the production of proteins in the male ejaculate that are important for induction of the PMR, and female fecundity increases with dietary protein while lifetime mating rate decreases...
April 14, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28412335/metabolic-syndrome-does-not-increase-the-risk-of-ejaculatory-dysfunction-in-patients-with-lower-urinary-tract-symptoms-and-benign-prostatic-enlargement-an-italian-single-center-cohort-study
#19
Cosimo De Nunzio, Riccardo Lombardo, Mauro Gacci, Antonio Nacchia, Fabrizio Presicce, Hassan Alkhatatbeh, Sergio Serni, Andrea Tubaro
OBJECTIVES: To evaluate the relationship between metabolic syndrome and ejaculatory dysfunction in patients with lower urinary tract symptoms (LUTS) and benign prostatic enlargement (BPE). METHODS: From 2012 to 2016, a consecutive series of men with LUTS and BPE attending our prostate clinic, evaluated with the International Prostatic Symptoms Score (IPSS) were enrolled into a prospective database. All patients were assessed using the short form of the International Index of Erectile Function (IIEF-SF) and the male sexual health questionnaire ejaculatory dysfunction short form (MSHQ-ejd-SF) which evaluates the ability to ejaculate, the ejaculation force, the ejaculation volume and subjective bother associated with ejaculatory dysfunction...
April 13, 2017: Urology
https://www.readbyqxmd.com/read/28410125/multi-omics-and-male-infertility-status-integration-and-future-prospects
#20
Ashima Sinha, Virendra Singh, Savita Yadav
Within the cell, gene expression analysis is the key to gain information about  different cellular and physiological events. The multifaceted route of fertilization is a combination of different processes, which include production, maturation and ejaculation of the sperm, its travel through the female genital tract, followed by the ultimate fusion of the fertile sperm with the egg. Early embryogenesis and gametogenesis as well as gene expression at tissue level and global gene silencing are under different levels of stringent epigenetic checks...
June 1, 2017: Frontiers in Bioscience (Scholar Edition)
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