keyword
https://read.qxmd.com/read/38526868/the-ecotin-like-peptidase-inhibitor-of-trypanosoma-cruzi-prevents-tmprss2-par2-tlr4-crosstalk-downmodulating-infection-and-inflammation
#1
JOURNAL ARTICLE
Tatiana F R Costa, Carolina M C Catta-Preta, Amy Goundry, Danielle B Carvalho, Nathalia S Rodrigues, Aislan C Vivarini, Mayra Fonseca de Abreu, Flavia C G Reis, Ana Paula C A Lima
Trypanosoma cruzi is the causative agent of Chagas disease, a chronic pathology that affects the heart and/or digestive system. This parasite invades and multiplies in virtually all nucleated cells, using a variety of host cell receptors for infection. T. cruzi has a gene that encodes an ecotin-like inhibitor of serine peptidases, ISP2. We generated ISP2-null mutants (Δisp2) in T. cruzi Dm28c using CRISPR/Cas9. Epimastigotes of Δisp2 grew normally in vitro but were more susceptible to lysis by human serum compared to parental and ISP2 add-back lines...
March 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38310331/retrospective-aprotinin-cardiac-surgical-studies-and-their-limitations-time-for-a-prospective-rct
#2
JOURNAL ARTICLE
Gabor Erdoes, Andreas Koster, Jerrold H Levy
No abstract text is available yet for this article.
February 3, 2024: European Journal of Cardio-thoracic Surgery
https://read.qxmd.com/read/38228104/sglt2-inhibitors-decrease-overhydration-and-proteasuria-in-patients-with-chronic-kidney-disease-a-longitudinal-observational-study
#3
JOURNAL ARTICLE
Anja Schork, Marie-Luise Eberbach, Bernhard N Bohnert, Matthias Wörn, David J Heister, Felix Eisinger, Elisabeth Vogel, Nils Heyne, Andreas L Birkenfeld, Ferruh Artunc
INTRODUCTION: SGLT2 inhibitors are used to reduce the risk of progression of chronic kidney disease (CKD). In patients with type 2 diabetes, they have been found to reduce extracellular volume. Given the high prevalence of extracellular volume expansion and overhydration in CKD, we investigated whether SGLT2 inhibitors might correct these disturbances in CKD patients. METHODS: CKD patients who started treatment with an SGLT2 inhibitor were investigated in this prospective observational study for 6 months...
January 16, 2024: Kidney & Blood Pressure Research
https://read.qxmd.com/read/38226724/pharmacological-interventions-for-the-prevention-of-bleeding-in-people-undergoing-elective-hip-or-knee-surgery-a-systematic-review-and-network-meta-analysis
#4
REVIEW
Victoria N Gibbs, Rita Champaneria, Josie Sandercock, Nicky J Welton, Louise J Geneen, Susan J Brunskill, Carolyn Dorée, Catherine Kimber, Antony Jr Palmer, Lise J Estcourt
BACKGROUND: Hip and knee replacement surgery is a well-established means of improving quality of life, but is associated with a significant risk of bleeding. One-third of people are estimated to be anaemic before hip or knee replacement surgery; coupled with the blood lost during surgery, up to 90% of individuals are anaemic postoperatively. As a result, people undergoing orthopaedic surgery receive 3.9% of all packed red blood cell transfusions in the UK. Bleeding and the need for allogeneic blood transfusions has been shown to increase the risk of surgical site infection and mortality, and is associated with an increased duration of hospital stay and costs associated with surgery...
January 16, 2024: Cochrane Database of Systematic Reviews
https://read.qxmd.com/read/38180872/use-of-aprotinin-versus-tranexamic-acid-in-cardiac-surgery-patients-with-high-risk-for-excessive-bleeding-apache-trial-a-multicentre-retrospective-comparative-non-randomised-historical-study
#5
JOURNAL ARTICLE
Eloïse Gallo, Philippe Gaudard, Sophie Provenchère, Fouzia Souab, Anaïs Schwab, Damien Bedague, Hugues de La Barre, Christian de Tymowski, Laysa Saadi, Bertrand Rozec, Bernard Cholley, Bruno Scherrer, Jean-Luc Fellahi, Alexandre Ouattara
OBJECTIVES: Following the reintroduction of aprotinin into the European market, the French Society of Cardiovascular and Thoracic Anaesthesiologists recommended its prophylactic use at half-dose for high-risk cardiac surgery patients. We examined whether the use of aprotinin instead of tranexamic acid could significantly reduces severe perioperative bleeding. METHODS: This multicentre, retrospective, historical study included cardiac surgery patients treated with aprotinin or tranexamic acid between December 2017 and September 2020...
January 5, 2024: European Journal of Cardio-thoracic Surgery
https://read.qxmd.com/read/38127365/preanalytical-impact-on-the-accuracy-of-measurements-of-glucagon-glp-1-and-gip-in-clinical-trials
#6
JOURNAL ARTICLE
Christine Rasmussen, Michael M Richter, Nicole J Jensen, Niklas Heinz, Bolette Hartmann, Jens J Holst, Sasha A S Kjeldsen, Nicolai J Wewer Albrechtsen
BACKGROUND: Plasma concentrations of glucagon, GLP-1 and GIP are reported in numerous clinical trials as outcome measures but preanalytical guidelines are lacking. We addressed the impact of commonly used blood containers in metabolic research on measurements of glucagon, GLP-1 and GIP in humans. METHODS: Seventeen overweight individuals were subjected to an overnight fast followed by an intravenous infusion of amino acids to stimulate hormonal secretion. Blood was sampled into five containers: EDTA-coated tubes supplemented with DMSO (control), a neprilysin inhibitor, aprotinin (a kallikrein inhibitor) or a DPP-4 inhibitor, and P800 tubes...
December 21, 2023: Scandinavian Journal of Clinical and Laboratory Investigation
https://read.qxmd.com/read/38074829/ulinastatin-in-combination-with-aprotinin-improves-systemic-inflammation-in-patients-undergoing-cardiac-surgery-with-cardiopulmonary-bypass
#7
JOURNAL ARTICLE
En Ma, Wensheng Chen, Chao Zhang, Caicheng Liu, Jian Lan, Xiyue Yan
OBJECTIVE: To investigate the impact of ulinastatin combined with protease inhibitors on serum inflammatory factors in patients undergoing cardiac surgery with cardiopulmonary bypass. METHODS: A retrospective analysis was conducted on 86 patients who underwent cardiac surgery with cardiopulmonary bypass at Xi'an Gaoxin Hospital from May 2019 to June 2021. Based on the administration of drugs by a micro-infusion pump after anesthesia induction and before skin incision, the patients were divided into an observation group (receiving ulinastatin at a dose of 12,000 U/kg and protease inhibitors at a dose of 4 million units) with 46 cases and a control group (receiving protease inhibitors at a dose of 2 million units) with 40 cases...
2023: American Journal of Translational Research
https://read.qxmd.com/read/38062077/platelet-activating-factor-and-protease-activated-receptor-2-cooperate-to-promote-neutrophil-recruitment-and-lung-inflammation-through-nuclear-factor-kappa-b-transactivation
#8
JOURNAL ARTICLE
Irismara Sousa Silva, Aline D Almeida, Antônio C M Lima Filho, Weslley Fernandes-Braga, Ayslan Barra, Hortência M C Oliveira, Geovanni D Cassali, Luciano S A Capettini, Gustavo B Menezes, Jacqueline I Alvarez-Leite, Maria F Leite, André Klein
Although it is well established that platelet-activated receptor (PAF) and protease-activated receptor 2 (PAR2) play a pivotal role in the pathophysiology of lung and airway inflammatory diseases, a role for a PAR2-PAFR cooperation in lung inflammation has not been investigated. Here, we investigated the role of PAR2 in PAF-induced lung inflammation and neutrophil recruitment in lungs of BALB/c mice. Mice were pretreated with the PAR2 antagonist ENMD1068, PAF receptor (PAFR) antagonist WEB2086, or aprotinin prior to intranasal instillation of carbamyl-PAF (C-PAF) or the PAR2 agonist peptide SLIGRL-NH2 (PAR2-AP)...
December 7, 2023: Scientific Reports
https://read.qxmd.com/read/38011893/catalytic-antibodies-may-contribute-to-demyelination-in-myalgic-encephalomyelitis-chronic-fatigue-syndrome
#9
JOURNAL ARTICLE
Michael Anthony Jensen, Miranda Lee Dafoe, Julie Wilhelmy, Layla Cervantes, Anna N Okumu, Lucas Kipp, Mohsen Nemat-Gorgani, Ronald Wayne Davis
Here we report preliminary data demonstrating that some patients with myalgic encephalomyelitis/chronic fatiguesyndrome (ME/CFS) may have catalytic autoantibodies that cause the breakdown of myelin basic protein (MBP). We propose that these MBP-degradative antibodies are important to the pathophysiology of ME/CFS, particularly in the occurrence of white matter disease/demyelination. This is supported by magnetic resonance imagining studies that show these findings in patients with ME/CFS and could explain symptoms of nerve pain and muscle weakness...
November 27, 2023: Biochemistry
https://read.qxmd.com/read/37807338/bone-marrow-concentration-in-combination-with-hyaluronan-and-fibrin-for-the-treatment-of-primary-and-non-primary-in-orthopaedic-cases-of-osteochondral-defects-of-the-ankle
#10
JOURNAL ARTICLE
Yang Li, Jun Wang, Ridong Tao, Xinwei Zhang
This research examined the effects of varying amounts of fibrinogen (the fibrin precursor) and HA-MA on mechanical strength, BMSC proliferation, and chondrogenesis potential in vitro. In order to start a culture, fibrinogen, aprotinin, doxycycline, and HA-MA were well mixed in 200 L of AMEM containing 1% (w/v) photoinitiator. In order to produce a fibrin gel, thrombin was used at 1, 3,5, and 7 days after implantation, live/dead staining and a metabolic-activity test were used to examine the viability and proliferation of BMSCs inside fibrin/HA-MA...
September 30, 2023: Cellular and Molecular Biology
https://read.qxmd.com/read/37641903/effectiveness-of-hemocoagulase-tranexamic-acid-and-their-combination-for-reducing-blood-loss-in-bimaxillary-orthognathic-surgery-a-retrospective-study
#11
JOURNAL ARTICLE
Min-Soo Kim, Se-Jin Han
OBJECTIVES: Orthognathic surgery is a corrective intervention for maxillofacial deformities. Bleeding is a major concern for oral and maxillofacial surgeons. Various agents, such as hemocoagulase, tranexamic acid, and aprotinin have been developed to reduce intraoperative bleeding and transfusion requirements. Therefore, in this study we aimed to investigate the effects of hemocoagulase and tranexamic acid, as well as their simultaneous use, to reduce bleeding during orthognathic surgery...
August 31, 2023: Journal of the Korean Association of Oral and Maxillofacial Surgeons
https://read.qxmd.com/read/37505868/measurement-of-ghrelin-as-a-marker-of-appetite-dysregulation-in-cats-with-and-without-chronic-kidney-disease
#12
JOURNAL ARTICLE
Katelyn Brusach, Sarah Lorbach, Jessica Quimby, Eline Nijveldt, Rene Paschall, Hannah Kinsella, Valerie Parker, Ramiro Toribio
Appetite abnormalities and weight loss are important comorbidities in the treatment of chronic kidney disease (CKD) in cats. Ghrelin, a key hormone involved in the regulation of appetite and metabolism, is a potential marker of appetite dysregulation in cats with CKD. The aim of this study was to compare the plasma concentrations of acylated, desacyl, and total ghrelin in normal cats and cats with CKD. Storage methodology was investigated prior to evaluating ghrelin concentrations in normal and CKD cats to facilitate clinical sample collection...
July 14, 2023: Veterinary Sciences
https://read.qxmd.com/read/37470128/characterization-of-digestive-proteases-in-the-gut-of-a-basal-deuterostome
#13
JOURNAL ARTICLE
Jasper Hildebrand, William W Chang, Marian Y Hu, Meike Stumpp
Digestive systems are complex organs that allow organisms to absorb energy from their environment to fuel vital processes like growth, development and the maintenance of homeostasis. A comprehensive understanding of digestive physiology is therefore essential to fully understand the energetics of an organism. The digestion of proteins is of particular importance because most heterotrophic organisms are not able to synthesize all essential amino acids. While Echinoderms are basal deuterostomes that share a large genetic similarity with vertebrates their digestion physiology remains largely unexplored...
July 20, 2023: Journal of Experimental Biology
https://read.qxmd.com/read/37446350/aprotinin-drug-against-respiratory-diseases
#14
REVIEW
Alexandre V Ivachtchenko, Andrey A Ivashchenko, Dmitrii O Shkil, Ilya A Ivashchenko
Aprotinin (APR) was discovered in 1930. APR is an effective pan-protease inhibitor, a typical "magic shotgun". Until 2007, APR was widely used as an antithrombotic and anti-inflammatory drug in cardiac and noncardiac surgeries for reduction of bleeding and thus limiting the need for blood transfusion. The ability of APR to inhibit proteolytic activation of some viruses leads to its use as an antiviral drug for the prevention and treatment of acute respiratory virus infections. However, due to incompetent interpretation of several clinical trials followed by incredible controversy in the literature, the usage of APR was nearly stopped for a decade worldwide...
July 6, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37371419/kinins-contribution-to-postoperative-pain-in-an-experimental-animal-model-and-its-implications
#15
JOURNAL ARTICLE
Indiara Brusco, Cássia Regina Silva, Juliano Ferreira, Sara Marchesan Oliveira
Postoperative pain causes discomfort and disability, besides high medical costs. The search for better treatments for this pain is essential to improve recovery and reduce morbidity and risk of chronic postoperative pain. Kinins and their receptors contribute to different painful conditions and are among the main painful inflammatory mediators. We investigated the kinin's role in a postoperative pain model in mice and reviewed data associating kinins with this painful condition. The postoperative pain model was induced by an incision in the mice's paw's skin and fascia with the underlying muscle's elevation...
June 12, 2023: Brain Sciences
https://read.qxmd.com/read/37316873/simultaneous-induction-of-vasculature-and-neuronal-network-formation-on-a-chip-reveals-a-dynamic-interrelationship-between-cell-types
#16
JOURNAL ARTICLE
Lotta Isosaari, Hanna Vuorenpää, Alma Yrjänäinen, Fikret Emre Kapucu, Minna Kelloniemi, Toni-Karri Pakarinen, Susanna Miettinen, Susanna Narkilahti
BACKGROUND: Neuronal networks receive and deliver information to regulate bodily functions while the vascular network provides oxygen, nutrients, and signaling molecules to tissues. Neurovascular interactions are vital for both tissue development and maintaining homeostasis in adulthood; these two network systems align and reciprocally communicate with one another. Although communication between network systems has been acknowledged, the lack of relevant in vitro models has hindered research at the mechanistic level...
June 14, 2023: Cell Communication and Signaling: CCS
https://read.qxmd.com/read/37272509/pharmacological-interventions-for-the-prevention-of-bleeding-in-people-undergoing-definitive-fixation-or-joint-replacement-for%C3%A2-hip-pelvic-and-long-bone-fractures
#17
REVIEW
Victoria N Gibbs, Rita Champaneria, Louise J Geneen, Parag Raval, Carolyn Dorée, Susan J Brunskill, Alex Novak, Antony Jr Palmer, Lise J Estcourt
BACKGROUND: Pelvic, hip, and long bone fractures can result in significant bleeding at the time of injury, with further blood loss if they are treated with surgical fixation. People undergoing surgery are therefore at risk of requiring a blood transfusion and may be at risk of peri-operative anaemia. Pharmacological interventions for blood conservation may reduce the risk of requiring an allogeneic blood transfusion and associated complications. OBJECTIVES: To assess the effectiveness of different pharmacological interventions for reducing blood loss in definitive surgical fixation of the hip, pelvic, and long bones...
June 5, 2023: Cochrane Database of Systematic Reviews
https://read.qxmd.com/read/37042750/identification-of-embryonic-chicken-proteases-activating-newcastle-disease-virus-and-their-roles-in-the-pathogenicity-of-virus-used-as-in-ovo-vaccine
#18
JOURNAL ARTICLE
Helong Feng, Lun Yao, Zhe Zeng, Liren Jiang, Yu Shang, Hongcai Wang, Li Li, Zichen Wang, Xin Wang, Hongchun Yang, Qingqing Zhao, Xiangfei Ren, Tengfei Zhang, Rongrong Zhang, Yunqing Guo, Qin Lu, Qiao Hu, Wenting Zhang, Chan Ding, Huabin Shao, Guofu Cheng, Qingping Luo, Guoyuan Wen
In ovo vaccination is an attractive immunization approach for chickens. However, most live Newcastle disease virus (NDV) vaccine strains used safely after hatching are unsafe as in ovo vaccines due to their high pathogenicity for chicken embryos. The mechanism for viral pathogenicity in chicken embryos is poorly understood. Our previous studies reported that NDV strain TS09-C was a safe in ovo vaccine, and the F protein cleavage site (FCS) containing three basic amino acids (3B-FCS) was the crucial determinant of the attenuation of TS09-C in chicken embryos...
May 31, 2023: Journal of Virology
https://read.qxmd.com/read/36894576/a-putative-cytotoxic-serine-protease-from-salmonella-typhimurium-ucb5-recovered-from-undercooked-burger
#19
JOURNAL ARTICLE
Essam Kotb, Baher A El-Nogoumy, Haifa A Alqahtani, Asmaa A Ahmed, Hussah A Al-Shwyeh, Sakina M Algarudi, Hanan Almahasheer
A putative virulence exoprotease designated as UcB5 was successfully purified from the bacterium Salmonella typhimurium to the electrophoretic homogeneity with 13.2-fold and 17.1% recovery by hydrophobic, ion-exchange, and gel permeation chromatography using Phenyl-Sepharose 6FF, DEAE-Sepharose CL-6B, and Sephadex G-75, respectively. By applying SDS-PAGE, the molecular weight was confirmed at 35 kDa. The optimal temperature, pH, and isoelectric point were 35 °C, 8.0, 5.6 ± 0...
March 9, 2023: Scientific Reports
https://read.qxmd.com/read/36867329/cost-analysis-of-aprotinin-reintroduction-in-french-cardiac-surgery-centres-a-real-world-data-based-analysis
#20
MULTICENTER STUDY
Pascal Colson, Jean-Luc Fellahi, Philippe Gaudard, Sophie Provenchère, Bertrand Rozec
INTRODUCTION: The European Medicines Agency restored aprotinin (APR) use for preventing blood loss in patients undergoing isolated coronary artery bypass graft (iCABG) in 2016 but requested the collection of patient and surgery data in a registry (NAPaR). The aim of this analysis was to evaluate the impact of APR reintroduction in France on the main hospital costs (operating room, transfusion and intensive unit stay) compared to the current use of tranexamic acid (TXA), which was the only antifibrinolytic available before APR reinstatement...
April 2023: Advances in Therapy
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