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https://www.readbyqxmd.com/read/28300392/generation-and-characterization-of-virus-infection-enhancing-peptide-nanofibrils-functionalized-with-fluorescent-labels
#1
Sascha Rode, Manuel Hayn, Annika Röcker, Stefanie Sieste, Marcus Lamla, Daniel Markx, Christoph Meier, Frank Kirchhoff, Paul Walther, Marcus Fändrich, Tanja Weil, Jan Muench
Retroviral gene transfer is the method of choice for the stable introduction of genetic material into the cellular genome. However, efficient gene transfer is often limited by low transduction rates of the viral vectors. We have recently described a 12-mer peptide, termed EF-C that forms amyloid-like peptide nanofibrils (PNF) strongly increasing viral transduction efficiencies. These nanofibrils are polycationic and bind negatively charged membranes of virions and cells, thereby overcoming charge repulsions and resulting in increased rates of virion attachment and gene transfer...
March 16, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28296683/preoperative-computed-tomography-assessment-for-perinephric-fat-invasion-comparison-with-pathological-staging
#2
Jaime Landman, Jae Young Park, Chenhui Zhao, Molly Baker, Martin Hofmann, Mohammad Helmy, Chandana Lall, Mari Bozoghlanian, Zhamshid Okhunov
OBJECTIVE: The aim of this study was to assess the accuracy of computed tomography (CT) imaging in diagnosing perinephric fat (PNF) invasion in patients with renal cell carcinoma. METHODS: We retrospectively reviewed the medical records and preoperative CT images of 161 patients (105 men and 56 women) for pT1-pT3a renal cell carcinoma. We analyzed the predictive accuracy of CT criteria for PNF invasion stratified by tumor size. We determined the predictive value of CT findings in diagnosing PNF invasion using logistic regression analysis...
March 15, 2017: Journal of Computer Assisted Tomography
https://www.readbyqxmd.com/read/28296134/predicting-primary-nonfunction-of-liver-transplants-with-laboratory-values-can-it-be-done
#3
EDITORIAL
Randall S Sung
Determination of primary nonfunction (PNF) of a liver transplant can be one of the most difficult assessments in transplantation. PNF can initially manifest in variety of ways both dramatic and subtle, and no one parameter is sufficient for diagnosis [1,2]. The determination of PNF informs the decision to relist for a second liver transplant. Once relisted, one or more additional confirmations are subsequently necessary in the context of deciding whether to retransplant as donors become available. In addition, a retransplant operation is not without risk, especially in the setting of the existing or impending multiorgan system failure that can be associated with PNF...
March 14, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28290498/inhibition-of-leukotriene-b4-receptor-1-attenuates-lipopolysaccharide-induced-cardiac-dysfunction-role-of-ampk-regulated-mitochondrial-function
#4
Meng Sun, Rui Wang, Qinghua Han
Leukotriene B4 (LTB4)-mediated leukocyte recruitment and inflammatory cytokine production make crucial contributions to chronic inflammation and sepsis; however, the role of LTB4 in lipopolysaccharide (LPS)-induced cardiac dysfunction remains unclear. Therefore, the present study addressed this issue using an LTB4 receptor 1 (BLT1) inhibitor. Administration of LPS to mice resulted in decreased cardiovascular function. Inhibition of LTB4/BLT1 with the BLT1 inhibitor U75302 significantly improved survival and attenuated the LPS-induced acute cardiac dysfunction...
March 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28290147/temporal-profile-and-severity-correlation-of-a-panel-of-rat-spinal-cord-injury-protein-biomarkers
#5
Zhihui Yang, Helen M Bramlett, Ahmed Moghieb, Dongnan Yu, Ping Wang, Fan Lin, Carl Bauer, Tyler M Selig, Emily Jaalouk, Amanda S Weissman, Disa S Rathore, Pammela Romo, Zhiqun Zhang, Ronald L Hayes, Michael Y Wang, W Dalton Dietrich, Kevin K W Wang
In the USA, there are approximately 12,000 new cases of spinal cord injury (SCI) each year and some 1.2 million people living with paralysis due to SCI. Seven percent of them are paralyzed due to an accident or injury occurring while serving in the military. Here, we report a systematic study on protein biomarker candidates in a rat SCI model with either moderate or severe injury. Tissue, cerebrospinal fluid (CSF), and serum samples were obtained at 4 h, 24 h, and 7 days post-injury. The candidate biomarkers included axonal injury markers αII-spectrin breakdown products (SBDP150/145/120), neuronal cell body injury marker ubiquitin C-terminal hydrolase-L1 (UCH-L1), astrogliosis/astroglial injury markers S100 calcium-binding protein-β (S100β), glial fibrillary acidic protein (GFAP) and GFAP breakdown products (GBDPs), demyelination marker myelin basic protein (MBP), axonal injury marker phosphorylated neurofilament-H (pNF-H), and neuroinflammation marker interleukin-6 (IL-6)...
March 13, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28287799/a-randomized-controlled-trial-of-a-web-based-personalized-normative-feedback-alcohol-intervention-for-young-adult-veterans
#6
Eric R Pedersen, Layla Parast, Grant N Marshall, Terry L Schell, Clayton Neighbors
OBJECTIVE: Young-adult American veterans are at risk for problematic alcohol use. However, they are unlikely to seek care and may drop out from lengthy, multicomponent treatments when they do get care. This randomized controlled trial tested a very brief alcohol intervention delivered over the Internet to reach the population of young-adult veterans to help reduce their drinking. METHOD: Veterans (N = 784) were recruited from Facebook and randomized to either a control condition or a personalized normative feedback (PNF) intervention seeking to correct drinking perceptions of gender-specific veteran peers...
March 13, 2017: Journal of Consulting and Clinical Psychology
https://www.readbyqxmd.com/read/28267886/primary-non-function-is-frequently-associated-with-fatty-liver-allografts-and-high-mortality-after-re-transplantation
#7
Ulf Kulik, Frank Lehner, Jürgen Klempnauer, Jürgen Borlak
BACKGROUND & AIMS: The shortage of liver donations demands the use of suboptimal grafts with steatosis being a frequent finding. Although ≤30% macrovesicular steatosis is considered to be safe the risk for primary non-function (PNF) and outcome after re-transplantation (re-OLT) is unknown. METHODS: Among 1205 orthotropic liver transplantations performed at our institution the frequency, survival and reason of re-OLT was evaluated. PNF (group A) cases and those with initial transplant function but subsequent need for re-OLT (group B) were analyzed...
March 7, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28264747/oocyte-morphology-and-embryo-morphokinetics-in-an-intra-cytoplasmic-sperm-injection-programme-is-there-a-relationship
#8
Azita Faramarzi, Mohammad Ali Khalili, Sareh Ashourzadeh
The aim was to investigate the relationship between the morphological parameters of metaphase II (MII) oocytes with morphokinetic variables of embryos following an intra-cytoplasmic sperm injection (ICSI) procedure. Morphokinetic behaviour and abnormal cleavage patterns of 334 zygotes were analyzed using time-lapse monitoring (TLM). In addition, oocyte morphology was assessed in relation to embryo morphokinetic (absolute time point, including time to second polar body (PB) extrusion (ESPB), pronuclei (PN) appearance (PNA), PN fading (PNF), time to 2-cells (t2), 3c (t3), 4c (t4), 5c (t5), 6c (t6), 7c (t7), 8c (t8) and relative timing parameters (S1, S2, CC2 and CC3)...
March 7, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28217149/congenital-extra-calvarial-plexiform-neurofibroma-in-occipito-cervical-region-with-occipital-bone-defect-with-neurofibromatosis-type-1-and-segmental-neurofibromatosis-case-report-and-review-of-literature
#9
REVIEW
Vivek Kumar Kankane, Gaurav Jaiswal, Tarun Kumar Gupta
Plexiform neurofibroma (PNF) of the scalp is an extremely rare lesion reported in association with neurofibromatosis (NF). Occipital location of PNF is even more infrequent; we reported one pediatric case of PNF in occipito-cervical region with multiple small occipital bone defects and associated with NF-1.
October 2016: Journal of Pediatric Neurosciences
https://www.readbyqxmd.com/read/28211034/infectious-complications-as-the-leading-cause-of-death-after-kidney-transplantation-analysis-of-more-than-10-000-transplants-from-a-single-center
#10
Flávio de Castro Rodrigues Ferreira, Marina Pontello Cristelli, Mayara Ivani Paula, Henrique Proença, Claudia Rosso Felipe, Helio Tedesco-Silva, José Osmar Medina-Pestana
AIM: To identify specific causes of graft failure in a large sample of kidney transplant patients from a middle-income, developing country. METHODS: Retrospective cohort study analyzing all consecutive single kidney transplants (KTs) performed at a single center in Brazil between January 1st 1998 and December 31st 2013. The database closing date was December 31st 2014. RESULTS: Out of 10,400 KTs, there were 1191 (11.45%) deaths with a functioning graft, 40 cases (0...
February 17, 2017: Journal of Nephrology
https://www.readbyqxmd.com/read/28210065/the-effects-of-therapeutic-exercise-using-pnf-on-the-size-of-calcium-deposits-pain-self-awareness-and-shoulder-joint-function-in-a-calcific-tendinitis-patient-a-case-study
#11
Dong-Gun Oh, Kyung-Tae Yoo
[Purpose] The purpose of this case study was to identify the effects of independent and intensive therapeutic exercise using Proprioceptive neuromuscular facilitation on the size of calcium deposits, pain self-awareness, and shoulder joint function in a patient with calcific tendonitis. [Subject and Methods] The subject was a 42-year-old female patient with calcific tendonitis and acute pain who had difficulty with active movement and problems with general function. The independent and intensive Proprioceptive neuromuscular facilitation exercise was applied for 40 min twice a day five times a week for two weeks for a total of 20 times...
January 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28199762/improving-the-diagnostic-criteria-for-primary-liver-graft-non-function-in-adults-utilizing-standard-and-transportable-laboratory-parameters-an-outcome-based-analysis
#12
Mohammad A B Al-Freah, Mark J W McPhail, Elena Dionigi, Matthew R Foxton, Georg Auzinger, Mohamed Rela, Julia A Wendon, John G O'Grady, Michael A Heneghan, Nigel D Heaton, William Bernal
Current diagnostic criteria for primary non-function (PNF) of liver grafts are based on clinical experience rather than statistical methods. A retrospective, single centre study was conducted of all adults (n=1,286) who underwent primary liver transplant 2000-2008 in our centre. Laboratory variable during the first post-liver transplant week were analysed. Forty two patients (3.7%) had 2-week graft failure. Transplant albumin, day-1 AST, day-1 lactate, day-3 bilirubin, day-3 INR and day-7 AST were independently associated with PNF on multivariate logistic regression...
February 15, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28198773/early-post-operative-results-of-percutaneous-needle-fasciotomy-in-451-patients-with-dupuytren-disease
#13
Sanne Molenkamp, Tanneke A M Schouten, Dieuwke C Broekstra, Paul M N Werker, J Daniel Moolenburgh
BACKGROUND: Percutaneous needle fasciotomy (PNF) is a minimally invasive treatment modality for Dupuytren disease (DD). In this study we analyzed the efficacy and complication-rate of PNF using a statistical method that takes the multi-level structure of data, regarding multiple measurements from the same patients, into account. METHODS: The data of 470 treated rays from 451 patients with Dupuytren disease that underwent percutaneous needle fasciotomy (PNF) were analyzed retrospectively...
February 13, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28192194/diagonal-movement-of-the-upper-limb-produces-greater-adaptive-plasticity-than-sagittal-plane-flexion-in-the-shoulder
#14
Rayele Moreira, Lysnara Lial, Maria Gabriela Teles Monteiro, Alice Aragão, Lorena Santos David, Marcelo Coertjens, Fernando L Silva-Júnior, Gildário Dias, Bruna Velasques, Pedro Ribeiro, Silmar Silva Teixeira, Victor Hugo Bastos
The motor rehabilitation is based on exercises that involve various joints and muscle groups. One such treatment method is Proprioceptive Neuromuscular Facilitation (PNF), which involves diagonal movements simulating many activities of daily living. The objective of this study was to investigate the differences between PNF and shoulder flexion movements performed without the diagonal component (i.e., only in the sagittal plane) using beta band absolute power as a measure of plasticity. The study included 30 volunteers randomized into three groups (control, PNF, and FLEX), with electroencephalographic signals captured before and after the performance of the task...
March 16, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28186658/cerebrospinal-fluid-levels-of-phosphorylated-neurofilament-heavy-as-a-diagnostic-marker-of-canine-degenerative-myelopathy
#15
C M Toedebusch, M D Bachrach, V B Garcia, G C Johnson, M L Katz, G Shaw, J R Coates, M L Garcia
BACKGROUND: No definitive, antemortem diagnostic test for canine degenerative myelopathy (DM) is available. Phosphorylated neurofilament heavy (pNF-H) is a promising biomarker for nervous system diseases. HYPOTHESIS/OBJECTIVE: Cerebrospinal fluid (CSF) and serum pNF-H is a detectable biological marker for diagnosis of canine DM. ANIMALS: Fifty-three DM-affected, 27 neurologically normal, 7 asymptomatic at-risk, and 12 DM mimic dogs. METHODS: Archived CSF and serum pNF-H concentrations were determined by a commercially available ELISA...
February 10, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28185258/cerebrospinal-fluid-levels-of-chromogranin-a-and-phosphorylated-neurofilament-heavy-chain-are-elevated-in-amyotrophic-lateral-sclerosis
#16
M Kaiserova, Z Grambalova, P Otruba, D Stejskal, H Prikrylova Vranova, J Mares, K Mensikova, P Kanovsky
BACKGROUND: Various cerebrospinal fluid (CSF) biomarkers are being studied to improve the sensitivity and specificity of the diagnostic methods for amyotrophic lateral sclerosis (ALS). AIMS OF THE STUDY: The aim of our study was to establish the CSF levels of chromogranin A (CgA) and phosphorylated neurofilament heavy chain (pNF-H) in patients with ALS in order to assess these proteins as possible biomarkers of ALS. METHODS: Cerebrospinal fluid levels of CgA and pNF-H were examined and mutually compared in 15 patients with sporadic ALS and 16 gender- and age-matched controls...
February 10, 2017: Acta Neurologica Scandinavica
https://www.readbyqxmd.com/read/28182516/the-effectiveness-of-pnf-vs-static-stretching-on-increasing-hip-flexion-range-of-motion
#17
Landon Lempke, Rebecca Green, Caitlin Murray, Justin Stanek
No abstract text is available yet for this article.
February 9, 2017: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/28179468/neurofilament-as-a-blood-marker-for-diagnosis-and-monitoring-of-primary-progressive-aphasias
#18
Petra Steinacker, Elisa Semler, Sarah Anderl-Straub, Janine Diehl-Schmid, Matthias L Schroeter, Ingo Uttner, Hans Foerstl, Bernhard Landwehrmeyer, Christine A F von Arnim, Jan Kassubek, Patrick Oeckl, Hans-Jürgen Huppertz, Klaus Fassbender, Klaus Fliessbach, Johannes Prudlo, Carola Roßmeier, Johannes Kornhuber, Anja Schneider, Alexander E Volk, Martin Lauer, Adrian Danek, Albert C Ludolph, Markus Otto
OBJECTIVE: To assess the utility of serum neurofilament for diagnosis and monitoring of primary progressive aphasia (PPA) variants. METHODS: We investigated neurofilament light chain (NF-L) levels in blood of 99 patients with PPA (40 with nonfluent variant PPA [nfvPPA], 38 with semantic variant PPA [svPPA], 21 with logopenic variant PPA [lvPPA]) and compared diagnostic performance with that reached by CSF NF-L, phosphorylated neurofilament heavy chain (pNF-H), β-amyloid (Aβ1-42), tau, and phosphorylated tau...
March 7, 2017: Neurology
https://www.readbyqxmd.com/read/28167188/the-effect-of-kinesio-taping-versus-stretching-techniques-on-muscle-soreness-and-flexibility-during-recovery-from-nordic-hamstring-exercise
#19
Tarik Ozmen, Gokce Yagmur Gunes, Hanife Dogan, Ilyas Ucar, Mark Willems
The purpose of this study was to examine the effects of static stretching, proprioceptive neuromuscular facilitation (PNF) stretching, or kinesio taping (KT) on muscle soreness and flexibility during recovery from exercise. Sixty-five females were randomly assigned to four groups: PNF stretching (n = 15), static stretching (n = 16), KT (n = 17), and control (n = 17). All participants performed nordic hamstring exercise (5 sets of 8 repetitions). In all groups, hamstring flexibility at 24 h and 48 h was not changed from baseline (p > ...
January 2017: Journal of Bodywork and Movement Therapies
https://www.readbyqxmd.com/read/28119879/influence-of-the-proprioceptive-neuromuscular-facilitation-exercise-programs-on-idiopathic-scoliosis-patient-in-the-early-20s-in-terms-of-curves-and-balancing-abilities-single-case-study
#20
Byung-Ki Lee
The purpose of this study was to determine the influence of the proprioceptive neuromuscular facilitation (PNF) exercise programs for idiopathic scoliosis with a female patient in the early 20s in terms of her spinal curve and balancing abilities. The study subject was selected among 21-year-old female college students. There were no particular activities that the subject could not perform, but patient complained of difficulty in maintaining the standing position for a prolonged time. Patient chest X-ray results showed S-shaped curves tilted towards the left or right in the lumbar spine, thoracic spine, and cervical spine areas...
December 2016: Journal of Exercise Rehabilitation
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