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Eunae Park, Yeojae Kim, Giltsu Choi
Phytochrome B (phyB) inhibits the function of phytochrome-interacting factors (PIFs) by inducing their degradation and sequestration, but the relative physiological importance of these two phyB activities is unclear. In an analysis of published Arabidopsis thaliana phyB mutations, we identified a point mutation in the N-terminal half of phyB (phyBG111D) that abolishes its PIF sequestration activity without affecting its PIF degradation activity. We also identified a point mutation in the phyB C-terminal domain, which, when combined with a deletion of the C-terminal end (phyB990G767R), does the opposite; it blocks PIF degradation without affecting PIF sequestration...
May 15, 2018: Plant Cell
Phunsup Wongsurakiat, Nadwipa Yuangtrakul
To determine the performance of bedside visual inspection of airway pressure-time (Paw-t) curve profiles (VI) for estimating stress index (SI) in patients with acute respiratory distress syndrome (ARDS). A prospective study in 30 subjects with ARDS receiving mechanical ventilation at two peak inspiratory flow (PIF) settings: 60 or 40 L/min. For each study session, two physicians inspected real-time Paw-t waveforms from mechanical ventilator's monitoring screens at bedside for 30 s and interpreted which of the three patterns (tidal recruitment, noninjurious ventilation or tidal overdistension) the Paw-t curve profile was compatible with...
May 9, 2018: Journal of Clinical Monitoring and Computing
Y Li, F Panhwa, Z Chen, F Yuan, X Ji, P Hu, G Zhao
  BACKGROUND: Intracellular ice formation (IIF) plays an important role in cryopreservation and cryosurgery. IIF in porcine iliac endothelial cells (PIECs) has not been fully investigated. OBJECTIVE: To analyze the phenomenon of IIF in PIECs during freezing. MATERIALS AND METHODS: The cryomicroscopy system was used for observation of the cell morphology and for the count of IIF during freezing, while the theoretical model for probability IIF (PIF) was used to determine the nucleation kinetic and thermodynamic parameters...
July 2017: Cryo Letters
Shuang Wu, Yujun Jiang, Binbin Lou, Jing Feng, Yanhong Zhou, Ling Guo, Stephen J Forsythe, Chaoxin Man
The air in a powdered infant formula (PIF) factory is a potential transfer medium for microorganisms. In this study, air samples from 6 main processing areas, almost covering the whole PIF processing line and 1 outdoor location, were collected from a PIF manufacturing plant during the winter and summer periods. A cultivation-based and an Illumina (San Diego, CA) high-throughput 16S rRNA sequencing method was used to investigate the community structures and distributions of bacteria in the air. High microbial diversity (25 genera, 56 species), with a dominant community including Staphylococcus, Bacillus, Acinetobacter, and Kocuria, was found by the cultivation-based method...
May 2, 2018: Journal of Dairy Science
Malesiba Mampotoko Selepe, Abraham Christoffel van Wyk, Kim Gilberte Pauline Hoek, Yvonne Prince, Paul David van Helden, Robin Mark Warren, Colleen Anne Wright
BACKGROUND: The rapid diagnosis of extrapulmonary tuberculosis in children remains challenging. The presence of enlarged lymph nodes provides an opportunity to obtain diagnostic material through fine needle aspiration biopsy (FNAB). Mycobacterial culture, traditionally the reference standard, has a slow turnaround time and PCR-based methods are not widely available in developing countries. Direct visualization of mycobacteria on microscopy can be a rapid method to confirm the diagnosis...
May 3, 2018: Diagnostic Cytopathology
Pieter C Barnhoorn, Mirjam Houtlosser, Marleen W Ottenhoff-de Jonge, Geurt T J M Essers, Mattijs E Numans, Anneke W M Kramer
The relatively new term "Professional Identity Formation" (PIF) complements behavior-based and attitude-based perspectives on professionalism. Unprofessional behavior and its remediation should also be addressed from this perspective. However, a framework is needed to guide discussion and remediation of unprofessional behavior, which can encompass behavior-based, attitude-based, and identity-based perspectives on professionalism. To this end, the authors propose a multi-level professionalism framework which describes, apart from professional behavior, more levels which influence professional performance: environment, competencies, beliefs, values, identity, and mission...
April 27, 2018: Medical Teacher
Qian-Feng Li, Jun Lu, Jia-Wen Yu, Chang-Quan Zhang, Jun-Xian He, Qiao-Quan Liu
BZR1 and BES1 are key transcription factors of brassinosteroid (BR) signaling and represent the integration node of numerous signaling cascades. Their direct target genes have been identified, and BZR1/BES1-DNA interactions have been experimentally verified. Importantly, BZR1/BES1 also integrate different growth and development events via direct protein-protein interactions. For instance, DELLAs, PIFs, ARF6, and PKL, all directly interact with BZR1/BES1, forming a BZR1/BES1-centered regulatory network to coordinate cell elongation...
April 16, 2018: Biochimica et Biophysica Acta
Jesper Beck Jørgensen, Peter Bondeven, Lene Hjerrild Iversen, Søren Laurberg, Bodil Ginnerup Pedersen
AIM: The aim of this prospective case-control study was to evaluate the rate of pelvic insufficiency fractures (PIF) in Denmark using magnetic resonance imaging (MRI) at 3-year follow-up. All patients had rectal cancer and had undergone surgery with or without preoperative chemo-radiotherapy (CRT). METHOD: Patients registered with primary rectal cancer in the Danish Colorectal Cancer Group database, who underwent rectal cancer resection from April 2011 through August 2012, were invited to participate in a national MRI study aiming to detect local recurrence and evaluate quality of the surgical treatment...
April 19, 2018: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
Carole Blay, Serge Planes, Chin-Long Ky
Nucleated pearls are produced by molluscs of the Pinctada genus through the biomineralisation activity of the pearl sac tissue within the recipient oyster. The pearl sac originates from graft tissue taken from the donor oyster mantle and its functioning is crucial in determining key factors that impact pearl quality surface characteristics. The specific role of related gene regulation during gem biogenesis was unknown, so we analysed the expression profiles of eight genes encoding nacreous (PIF, MSI60, PERL1) or prismatic (SHEM5, PRISM, ASP, SHEM9) shell matrix proteins or both (CALC1) in the pearl sac (N = 211) of Pinctada margaritifera during pearl biogenesis...
April 16, 2018: Marine Biotechnology
Rui Zhang, Mengting Qin, Jie Shi, Lu Tan, Jiamin Xu, Zhenyan Tian, Yuhui Wu, Yuxuan Li, Yitian Li, Ning Wang
In the present study, the Pif gene of the freshwater pearl aquaculture mussel, Hyriopsis cumingii (HcPif) was successfully cloned and functionally characterized. The full sequence of HcPif gene consists of 3415 base pairs, which putatively encode two proteins, HcPif90 and HcPif80. A sequence analysis revealed that HcPif contained a von Willebrand factor type A domain and a chitin-binding domain, and shared many functional residues with other Pif homologues. A highly conserved sequence, FKGLDEIELML, at the C-terminus of Pif80s was identified as the key functional site...
April 2018: 3 Biotech
(no author information available yet)
No abstract text is available yet for this article.
April 2018: Australasian Psychiatry: Bulletin of Royal Australian and New Zealand College of Psychiatrists
Rabeb Bennour Hennekinne, Laurent Guillier, Lindsay Fazeuilh, Timothy Ells, Stephen Forsythe, Emily Jackson, Thomas Meheut, Nathalie Gnanou Besse
Cronobacter is a ubiquitous Gram-negative pathogen bacterium capable of surviving in low water activity environments, in particular powdered infant formula (PIF). Seven Cronobacter strains representing four different species (C. sakazakii, n=4; C. malonaticus, n=1; C. muytjensii, n=1; C. turicensis, n=1) were subjected to dry stress and stored in PIF at room temperature. The resulting survivor curves showed that Cronobacter spp. can survive for extended periods of at least three months with a significant, but moderate, variability regarding the level of resistance between species; however, no correlation was evident regarding the origin of strains...
March 25, 2018: Letters in Applied Microbiology
Jesse J Liu, Jeffrey S Raskin, Fran Hardaway, Katherine Holste, Sarah Brown, Ahmed M Raslan
BACKGROUND: Delivery of higher value healthcare is an ultimate government and public goal. Improving efficiency by standardization of surgical steps can improve patient outcomes, reduce costs, and lead to higher value healthcare. Lean principles and methodology have improved timeliness in perioperative medicine; however, process mapping of surgery itself has not been performed. OBJECTIVE: To apply Plan/Do/Study/Act (PDSA) cycles methodology to lumbar posterior instrumented fusion (PIF) using lean principles to create a standard work flow, identify waste, remove intraoperative variability, and examine feasibility among pilot cases...
March 14, 2018: Operative Neurosurgery (Hagerstown, Md.)
George Alliwa Makalliwa, Xi Wang, Huanyu Zhang, Nan Zhang, Cheng Chen, Jiang Li, Fei Deng, Hualin Wang, Manli Wang, Zhihong Hu
Effective oral infection is set off by interaction of a group of conserved per os infectivity factors (PIFs) with larval midgut columnar epithelial cells. We constructed pseudotyped viruses by substituting pif1, pif2 or pif3 genes of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) with their homologs from Mamestra bracissae multiple nucleopolyhedrovirus and tested their infectivity to tissue culture cells and to larvae. Transfection and infection assays revealed that all recombinant viruses generated infectious budded virus in both cell culture and in larvae...
March 16, 2018: Virologica Sinica
Siying Liu, Yunyun Geng, Libing Liu, Xiaoxia Sun, Jingyu Shao, Beibei Han, Jianchang Wang, Ke Tan
Cronobacter spp. is an opportunistic pathogen that is associated with rare but life-threatening neonatal infections resulting from the consumption of contaminated powdered infant formula milk (PIF). In the present study, we developed recombinase polymerase amplification (RPA) and real-time RPA for the detection of Cronobacter spp. in PIF for the first time by targeting the ompA gene. The specificity and sensitivity of the RPA and real-time RPA were validated and the practical applicability of these methods for the detection of Cronobacter spp...
March 7, 2018: Journal of Dairy Science
Åsa Lidén, Tine Veronika Karlsen, Bengt Guss, Rolf K Reed, Kristofer Rubin
NEW FINDINGS: What is the central question of this study? Collagen-binding β1 -integrins function physiologically in cellular control of dermal interstitial fluid pressure (PIF ) in vivo and thereby participate in control of extravascular fluid volume. During anaphylaxis, simulated by injection of compound 48/80, integrin αV β3 takes over this physiological function. Here we addressed the question whether integrin αV β3 can replace collagen-binding β1 -integrin to maintain a long-term homeostatic PIF ...
May 1, 2018: Experimental Physiology
Annette Kainu, Kirsi L Timonen, Esko Vanninen, Anssi R Sovijärvi
Inspiratory spirometry is used in evaluation of upper airway disorders e.g. fixed or variable obstruction. There are, however, very few published data on normal values for inspiratory spirometry. The main aim of this study was to produce reference values for inspiratory spirometry for healthy Finnish adults. Inspiratory spirometry was preplanned to a sample of the Finnish spirometry reference values sample. Data was successfully retrieved from 368 healthy nonsmoking adults (132 males) between 19 and 83 years of age...
March 7, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Ruili Pan, Yujun Jiang, Luhong Sun, Rui Wang, Kejin Zhuang, Yueming Zhao, Hui Wang, Md Aslam Ali, Honghua Xu, Chaoxin Man
Cronobacter sakazakii is an opportunistic foodborne pathogen that can infect newborns through powdered infant formula (PIF). In this study, we developed a novel enhanced lateral flow immunoassay (LFA) with enhanced sensitivity for detection of C. sakazakii in PIF by the naked eye. The proposed strategy for signal enhancement of the traditional LFA used concentrated gold nanoparticles (AuNP) as the enhancer to conjugate with capture antibodies, which could increase the immobilized capture antibodies concentration at the detection zone to improve capture efficiency...
May 2018: Journal of Dairy Science
Chirag Bhat, Sarah Burm, Tricia Mohan, Saad Chahine, Mark Goldszmidt
OBJECTIVES: Socialisation theories of professional identity formation (PIF) consider clinical rotations to be critically intense transformative experiences. However, few studies have explored what trainees grapple with during these transformative experiences or their influence on performance. Applying a threshold concepts (TCs) lens, this study investigates and documents 'troublesome' and 'transformative' concepts that junior trainees may encounter during a clinical rotation. Insights gained are essential for supporting trainee development...
February 27, 2018: Medical Education
Anshulika Sable, Krishan M Rai, Amit Choudhary, Vikash K Yadav, Sudhir K Agarwal, Samir V Sawant
Cotton fiber is a specialized unicellular structure useful for the study of cellular differentiation and development. Heat shock proteins (HSPs) have been shown to be involved in various developmental processes. Microarray data analysis of five Gossypium hirsutum genotypes revealed high transcript levels of GhHSP90 and GhHSP70 genes at different stages of fiber development, indicating their importance in the process. Further, we identified 26 and 55 members of HSP90 and HSP70 gene families in G. hirsutum. The treatment of specific inhibitors novobiocin (Nov; HSP90) and pifithrin/2-phenylethynesulfonamide (Pif; HSP70) in in-vitro cultured ovules resulted in a fewer number of fiber initials and retardation in fiber elongation...
February 26, 2018: Scientific Reports
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