Read by QxMD icon Read


Baoqing Zhou, Bolu Chen, Xin Wu, Fan Li, Pei Yu, Zoraida P Aguilar, Hua Wei, Hengyi Xu
A rapid, reliable, and sensitive method for the detection of Cronobacter sakazakii, a common foodborne pathogen that may cause serious neonatal disease, has been developed. In this study, a rapid real-time quantitative PCR (qPCR) assay combined with sodium deoxycholate (SD) and propidium monoazide (PMA) was developed to detect C. sakazakii contamination in powdered infant formula (PIF). This method could eliminate the interference from dead or injured bacteria. Optimization studies indicated that SD and PMA at 0...
October 19, 2016: Journal of Dairy Science
Dmitry A Argunov, Vadim B Krylov, Nikolay E Nifantiev
A new pyranoside-into-furanoside (PIF) rearrangement of selectively protected galactopyranosides, followed by controlled O(5) → O(6) benzoate migration, gives either 5-OH or 6-OH products. It has been applied for the synthesis of four oligosaccharides related to the galactomannan from Aspergillus fumigatus. The assembly of target oligosaccharides containing both (1→5) and (1→6) linkages between galactofuranosyl residues was performed by applying terminal mannoside and digalactofuranoside blocks, forming a versatile approach toward fungal and bacterial carbohydrate antigens containing both 5-O- and 6-O-substituted galactofuranoside residues...
October 19, 2016: Organic Letters
Jaewook Kim, Kijong Song, Eunae Park, Keunhwa Kim, Gabyong Bae, Giltsu Choi
Seedling hypocotyls display negative gravitropism in the dark but agravitropism in the light. The Arabidopsis thaliana pif quadruple mutant (pifQ), which lacks four PHYTOCHROME-INTERACTING FACTORS (PIFs), is agravitropic in the dark. Endodermis-specific expression of PIF1 rescues gravitropism in pifQ mutant seedlings. Since phytochromes induce light responses by inhibiting PIFs and the COP1-SPA ubiquitin E3 ligase complex in the nucleus, we asked whether phyB can cell autonomously inhibit hypocotyl negative gravitropism in the endodermis...
October 6, 2016: Plant Cell
Cong Ming-Hua, Zou Bao-Hua, Yu Lei
Anorexia cancer cachexia syndrome is prevalent in advanced cancer patients, which is featured by anorexia, decreased dietary intake, body weight loss (skeletal muscle mass loss), and unable to be reversed by routine nutritional support therapy. Up to now, the main mechanisms involved in cancer cachexia include excessive systemic inflammation, which is represented by increased plasma levels of IL-1, IL-6, TNF-alpha, tumor-induced factors, such as PIF and LMF. These factors eventually act on orexigenic and anorexigenic neurons located in hypothalamus or protein and lipid metabolism of peripheral tissues, which lead to anorexia, decreased dietary intake, enhanced basic metabolism rate and hyper catabolism...
October 18, 2016: Current Pharmaceutical Biotechnology
Svatopluk Binder, Adéla Hanáková, Kristína Tománková, Klára Pížová, Robert Bajgar, Barbora Manišová, Kristina Kejlová, Hana Bendová, Dagmar Jírová, Hana Kolářová
AIM: Natural or artificial substances have become an inseparable part of our lives. It is questionable whether adequate testing has been performed in order to ensure these substances do not pose a serious health risk. The principal aim of our research was to clarify the potential risk of adding essential oils to food, beverages and cosmetic products. METHODS: The toxicity of substances frequently employed in cosmetics, aromatherapy and food industry (bergamot oil, Litsea cubeba oil, orange oil, citral) were investigated using cell line NIH3T3 (mouse fibroblasts) with/without UV irradiation...
September 2016: Central European Journal of Public Health
Shruti Shukla, Gibaek Lee, Xinjie Song, Jung Hyun Park, Hyunjeong Cho, Eun Ju Lee, Myunghee Kim
This study aimed to optimize the applicability of an immunoliposome-based immunomagnetic concentration and separation assay to facilitate rapid detection of Cronobacter sakazakii in powdered infant formula (PIF). To determine the detection limit, specificity, and pre-enrichment incubation time (0, 4, 6, and 8 h), assay tests were performed with different cell numbers of C. sakazakii (2 × 10(0) and 2 × 10(1) CFU/ml) inoculated in 10 g of PIF. The assay was able to detect as few as 2 cells of C...
October 10, 2016: Scientific Reports
Daniela Liebsch, Olivier Keech
563 I. 563 II. 564 III. 564 IV. 565 V. 565 VI. 567 VII. 567 568 References 568 SUMMARY: Leaf senescence - the coordinated, active process leading to the organized dismantling of cellular components to remobilize resources - is a fundamental aspect of plant life. Its tight regulation is essential for plant fitness and has crucial implications for the optimization of plant productivity and storage properties. Various investigations have shown light deprivation and light perception via phytochromes as key elements modulating senescence...
November 2016: New Phytologist
Ashley Rosko, Hai-Lin Wang, Marcos de Lima, Brenda Sandmaier, H Jean Khoury, Andrew Artz, Johnathan Brammer, Christopher Bredeson, Sherif Farag, Mohamed Kharfan-Dabaja, Hillard M Lazarus, David I Marks, Rodrigo Martino Bufarull, Joseph McGuirk, Mohamed Mohty, Taiga Nishihori, Ian Nivison-Smith, Armin Rashidi, Olle Ringden, Matthew Seftel, Daniel Weisdorf, Veronika Bachanova, Wael Saber
: Older adults with B-cell acute lymphoblastic leukemia (B-ALL) have poor survival. We examined the effectiveness of reduced intensity conditioning (RIC) hematopoietic cell transplant (HCT) in adults with B-ALL age 55 years and older and explored prognostic factors associated with long-term outcomes. METHODS: Using CIBMTR registry data, we evaluated 273 patients (median age 61, range 55-72) with B-ALL with disease status in CR1 (71%), >CR2 (17%) and Primary Induction Failure (PIF)/Relapse (11%), who underwent RIC HCT between 2001-2012 using mostly unrelated donor (59%) or HLA-matched sibling (32%)...
October 6, 2016: American Journal of Hematology
S B Im, S-J Kwon, J Ryu, S W Jeong, J B Kim, J-W Ahn, S H Kim, Y D Jo, H-I Choi, S-Y Kang
Under certain circumstances, transposable elements (TE) can create or reverse mutations and alter the genome size of a cell. Sorghum (Sorghum bicolor L.) is promising for plant transposon tagging due to its small genome size and its low content of repetitive DNA. We developed a marker system based on targeted region amplification polymorphisms (TE-TRAP) that uses the terminal inverted repeats (TIRs) of transposons. A total of 3816 class 2 transposons belonging to the PIF/Harbinger family were identified from the whole sorghum genome that produced five primers, including eight types of TIRs...
September 16, 2016: Genetics and Molecular Research: GMR
Jörg O Schulze, Giorgio Saladino, Katrien Busschots, Sonja Neimanis, Evelyn Süß, Dalibor Odadzic, Stefan Zeuzem, Valerie Hindie, Amanda K Herbrand, María-Natalia Lisa, Pedro M Alzari, Francesco L Gervasio, Ricardo M Biondi
Allostery is a phenomenon observed in many proteins where binding of a macromolecular partner or a small-molecule ligand at one location leads to specific perturbations at a site not in direct contact with the region where the binding occurs. The list of proteins under allosteric regulation includes AGC protein kinases. AGC kinases have a conserved allosteric site, the phosphoinositide-dependent protein kinase 1 (PDK1)-interacting fragment (PIF) pocket, which regulates protein ATP-binding, activity, and interaction with substrates...
October 20, 2016: Cell Chemical Biology
Madoka Kudo, Satoshi Kidokoro, Takuya Yoshida, Junya Mizoi, Daisuke Todaka, Alisdair R Fernie, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
Although a variety of transgenic plants that are tolerant to drought stress have been generated, many of these plants show growth retardation. To improve drought tolerance and plant growth, we applied a gene-stacking approach using two transcription factor genes: DEHYDRATION-RESPONSIVE ELEMENT-BINDING 1A (DREB1A) and rice PHYTOCHROME-INTERACTING FACTOR-LIKE 1 (OsPIL1). The overexpression of DREB1A has been reported to improve drought stress tolerance in various crops, although it also causes a severe dwarf phenotype...
September 29, 2016: Plant Biotechnology Journal
Lluís Cordón-Barris, Sònia Pascual-Guiral, Shaobin Yang, Lydia Giménez-Llort, Silvia Lope-Piedrafita, Carlota Niemeyer, Enrique Claro, Jose M Lizcano, Jose R Bayascas
The phosphoinositide 3-kinase (PI 3-kinase)/Akt signaling pathway plays essential roles during neuronal development. The 3-phosphoinositide-dependent protein kinase 1 (PDK1) coordinates the PI 3-kinase signals by activating twenty three kinases of the AGC family including Akt. Phosphorylation of a conserved docking site in the substrate is a requisite for PDK1 to recognize, phosphorylate and activate most of these kinases, with the exception of Akt. We exploited this differential mechanism of regulation by generating neuronal-specific conditional knock-in mice expressing the mutant form of PDK1 L155E in which the substrate-docking site binding motif, termed the PIF-pocket, was disrupted...
September 19, 2016: Molecular and Cellular Biology
Haixia Zhou, Changcheng Zheng, Xiaoyu Zhu, Baolin Tang, Juan Tong, Xuhan Zhang, Lei Zhang, Huilan Liu, Zimin Sun
No clinical studies have investigated the role of decitabine as a part of the myeloablative conditioning regimen prior to UCBT for refractory or relapsed childhood AL in patients in NR status. The aim of this study was to identify the potential benefits of decitabine as a prior therapy before salvaged unrelated UCBT for refractory or relapsed childhood AL. Eight consecutive patients with childhood refractory/relapsed AL were enrolled in our study between 2013 and 2014. All patients were in NR status before the time of transplant and had features associated with poor outcomes, such as CNSL, MDS-AML, high WBC count at diagnosis, and hypodiploid status (FLT3+/ITD+)...
September 12, 2016: Pediatric Transplantation
Faysal A Yafi, Kenneth J DeLay, Carrie Stewart, Jason Chiang, Premsant Sangkum, Wayne J G Hellstrom
PURPOSE: The AMS 800(TM) artificial urinary sphincter (AUS) remains the gold standard for the surgical management of male stress urinary incontinence. We reviewed AUS device survival following primary implantation. MATERIALS AND METHODS: Retrospective data was collected from the AMS 800(TM) patient information form (PIF) database. Since 1972, 77,512 PIFs for primary AUS implantation have been completed in the United States. Following exclusion of procedures performed in children and females, and those labeled with an unknown surgical technique, 27,096 AUS cases were included in the analysis...
September 7, 2016: Journal of Urology
Huaning Zhang, Peibin Hou, Hui Lv, Yuzhen Chen, Xinpeng Li, Yanyan Ren, Mei Wang, Hailian Tan, Zhenwang Bi
BACKGROUND: Infection with Cronobacter spp. leads to neonatal meningitis, necrotizing enterocolitis and bacteremia. Cronobacter spp. has been reported to be an important pathogen contaminating powdered infant formula (PIF) and Follow-up formula (FUF), but little is known about the contamination level of Cronobacter spp. in PIFs and FUFs in China. RESULTS: 1032 samples were collected between 2011 and 2013. 42 samples were positive including 1.6% in PIFs and 6.5% in FUFs...
September 4, 2016: Journal of the Science of Food and Agriculture
Keunhwa Kim, Jinkil Jeong, Jeongheon Kim, Nayoung Lee, Mi Eon Kim, Sangil Lee, Sun Chang Kim, Giltsu Choi
Mutations in Phytochrome Interacting Factors (PIFs) induce a conversion of the endodermal amyloplasts necessary for gravity sensing to plastids with developed thylakoids accompanied by abnormal activation of photosynthetic genes in the dark. In this study, we investigated how PIFs regulate endodermal plastid development by performing comparative transcriptome analysis. We show that both endodermal expression of PIF1 and global expression of the PIF quartet induce transcriptional changes in genes enriched for nuclear-encoded photosynthetic genes such as LHCA and LHCB...
October 10, 2016: Molecular Plant
Robin C Willenbring, Fang Jin, David J Hinton, Mike Hansen, Doo-Sup Choi, Kevin D Pavelko, Aaron J Johnson
BACKGROUND: CD8 T cell-mediated blood-brain barrier (BBB) disruption is dependent on the effector molecule perforin. Human perforin has extensive single nucleotide variants (SNVs), the significance of which is not fully understood. These SNVs can result in reduced, but not ablated, perforin activity or expression. However, complete loss of perforin expression or activity results in the lethal disease familial hemophagocytic lymphohistiocytosis type 2 (FHL 2). In this study, we address the hypothesis that a single perforin allele can alter the severity of BBB disruption in vivo using a well-established model of CNS vascular permeability in C57Bl/6 mice...
2016: Journal of Neuroinflammation
Yuyu Zheng, Zhiping Gao, Ziqiang Zhu
DELLA proteins are central regulators in gibberellin (GA) signaling that interact with PHYTOCHROME-INTERACTING FACTORS (PIFs) and block their DNA-binding capacity, impairing their function in integrating light signals. A recent report demonstrates that DELLAs also negatively control PIF stability, thus providing a new layer of coordination of light and GA signaling.
October 2016: Trends in Plant Science
Susan A Csiszar, Alexi S Ernstoff, Peter Fantke, David E Meyer, Olivier Jolliet
We demonstrate the application of a high-throughput modeling framework to estimate exposure to chemicals used in personal care products (PCPs). As a basis for estimating exposure, we use the product intake fraction (PiF), defined as the mass of chemical taken by an individual or population per mass of a given chemical used in a product. We calculated use- and disposal- stage PiFs for 518 chemicals for five PCP archetypes. Across all product archetypes the use- and disposal- stage PiFs ranged from 10(-5) to 1 and 0 to 10(-3), respectively...
November 2016: Chemosphere
David Solomon, Bo-Christer Björk
Background. Open access (OA) publishing via article processing charges (APCs) is growing as an alternative to subscription publishing. The Pay It Forward (PIF) Project is exploring the feasibility of transitioning from paying subscriptions to funding APCs for faculty at research intensive universities. Estimating of the cost of APCs for the journals authors at research intensive universities tend to publish is essential for the PIF project and similar initiatives. This paper presents our research into this question...
2016: PeerJ
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"