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Drosophila myofibril

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https://www.readbyqxmd.com/read/27783625/zasp52-a-core-z-disc-protein-in-drosophila-indirect-flight-muscles-interacts-with-%C3%AE-actinin-via-an-extended-pdz-domain
#1
Kuo An Liao, Nicanor González-Morales, Frieder Schöck
Z-discs are organizing centers that establish and maintain myofibril structure and function. Important Z-disc proteins are α-actinin, which cross-links actin thin filaments at the Z-disc and Zasp PDZ domain proteins, which directly interact with α-actinin. Here we investigate the biochemical and genetic nature of this interaction in more detail. Zasp52 is the major Drosophila Zasp PDZ domain protein, and is required for myofibril assembly and maintenance. We show by in vitro biochemistry that the PDZ domain plus a C-terminal extension is the only area of Zasp52 involved in the interaction with α-actinin...
October 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27765651/metamorphosis-of-the-drosophila-visceral-musculature-and-its-role-in-intestinal-morphogenesis-and-stem-cell-formation
#2
Patrick Aghajanian, Shigeo Takashima, Manash Paul, Amelia Younossi-Hartenstein, Volker Hartenstein
The visceral musculature of the Drosophila intestine plays important roles in digestion as well as development. Detailed studies investigating the embryonic development of the visceral muscle exist; comparatively little is known about postembryonic development and metamorphosis of this tissue. In this study we have combined the use of specific markers with electron microscopy to follow the formation of the adult visceral musculature and its involvement in gut development during metamorphosis. Unlike the adult somatic musculature, which is derived from a pool of undifferentiated myoblasts, the visceral musculature of the adult is a direct descendant of the larval fibers, as shown by activating a lineage tracing construct in the larval muscle and obtaining labeled visceral fibers in the adult...
December 1, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27451905/lamin-mutations-accelerate-aging-via-defective-export-of-mitochondrial-mrnas-through-nuclear-envelope-budding
#3
Yihang Li, Linda Hassinger, Travis Thomson, Baojin Ding, James Ashley, William Hassinger, Vivian Budnik
Defective RNA metabolism and transport are implicated in aging and degeneration [1, 2], but the underlying mechanisms remain poorly understood. A prevalent feature of aging is mitochondrial deterioration [3]. Here, we link a novel mechanism for RNA export through nuclear envelope (NE) budding [4, 5] that requires A-type lamin, an inner nuclear membrane-associated protein, to accelerated aging observed in Drosophila LaminC (LamC) mutations. These LamC mutations were modeled after A-lamin (LMNA) mutations causing progeroid syndromes (PSs) in humans...
August 8, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/27107639/a-restrictive-cardiomyopathy-mutation-in-an-invariant-proline-at-the-myosin-head-rod-junction-enhances-head-flexibility-and-function-yielding-muscle-defects-in-drosophila
#4
Madhulika Achal, Adriana S Trujillo, Girish C Melkani, Gerrie P Farman, Karen Ocorr, Meera C Viswanathan, Gaurav Kaushik, Christopher S Newhard, Bernadette M Glasheen, Anju Melkani, Jennifer A Suggs, Jeffrey R Moore, Douglas M Swank, Rolf Bodmer, Anthony Cammarato, Sanford I Bernstein
An "invariant proline" separates the myosin S1 head from its S2 tail and is proposed to be critical for orienting S1 during its interaction with actin, a process that leads to muscle contraction. Mutation of the invariant proline to leucine (P838L) caused dominant restrictive cardiomyopathy in a pediatric patient (Karam et al., Congenit. Heart Dis. 3:138-43, 2008). Here, we use Drosophila melanogaster to model this mutation and dissect its effects on the biochemical and biophysical properties of myosin, as well as on the structure and physiology of skeletal and cardiac muscles...
June 5, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/26956799/profilin-modulates-sarcomeric-organization-and-mediates-cardiomyocyte-hypertrophy
#5
Viola Kooij, Meera C Viswanathan, Dong I Lee, Peter P Rainer, William Schmidt, William A Kronert, Sian E Harding, David A Kass, Sanford I Bernstein, Jennifer E Van Eyk, Anthony Cammarato
AIMS: Heart failure is often preceded by cardiac hypertrophy, which is characterized by increased cell size, altered protein abundance, and actin cytoskeletal reorganization. Profilin is a well-conserved, ubiquitously expressed, multifunctional actin-binding protein, and its role in cardiomyocytes is largely unknown. Given its involvement in vascular hypertrophy, we aimed to test the hypothesis that profilin-1 is a key mediator of cardiomyocyte-specific hypertrophic remodelling. METHODS AND RESULTS: Profilin-1 was elevated in multiple mouse models of hypertrophy, and a cardiomyocyte-specific increase of profilin in Drosophila resulted in significantly larger heart tube dimensions...
May 15, 2016: Cardiovascular Research
https://www.readbyqxmd.com/read/26823289/e2f-function-in-muscle-growth-is-necessary-and-sufficient-for-viability-in-drosophila
#6
Maria Paula Zappia, Maxim V Frolov
The E2F transcription factor is a key cell cycle regulator. However, the inactivation of the entire E2F family in Drosophila is permissive throughout most of animal development until pupation when lethality occurs. Here we show that E2F function in the adult skeletal muscle is essential for animal viability since providing E2F function in muscles rescues the lethality of the whole-body E2F-deficient animals. Muscle-specific loss of E2F results in a significant reduction in muscle mass and thinner myofibrils...
2016: Nature Communications
https://www.readbyqxmd.com/read/26446785/a-failure-to-communicate-myosin-residues-involved-in-hypertrophic-cardiomyopathy-affect-inter-domain-interaction
#7
William A Kronert, Girish C Melkani, Anju Melkani, Sanford I Bernstein
Our molecular modeling studies suggest a charge-dependent interaction between residues Glu-497 in the relay domain and Arg-712 in the converter domain of human β-cardiac myosin. To test the significance of this putative interaction, we generated transgenic Drosophila expressing indirect flight muscle myosin with charge reversal mutations in the relay (E496R) or converter (R713E). Each mutation yielded dramatic reductions in myosin Ca-ATPase activity (~80%) as well as in basal (~67%) and actin-activated (~84%) Mg-ATPase activity...
December 4, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26305467/the-regulation-of-muscle-structure-and-metabolism-by-mio-dchrebp-in-drosophila
#8
Grzegorz L Polak, Anthony Pasqualino, James E B Docherty, Stephen J Beck, Justin R DiAngelo
All cells require energy to perform their specialized functions. Muscle is particularly sensitive to the availability of nutrients due to the high-energy requirement for muscle contraction. Therefore the ability of muscle cells to obtain, store and utilize energy is essential for the function of these cells. Mio, the Drosophila homolog of carbohydrate response element binding protein (ChREBP), has recently been identified as a nutrient responsive transcription factor important for triglyceride storage in the fly fat body...
2015: PloS One
https://www.readbyqxmd.com/read/26110760/talin-is-required-continuously-for-cardiomyocyte-remodeling-during-heart-growth-in-drosophila
#9
Simina Bogatan, Duygu Cevik, Valentin Demidov, Jessica Vanderploeg, Abdullah Panchbhaya, Alex Vitkin, J Roger Jacobs
Mechanotransduction of tension can govern the remodeling of cardiomyocytes during growth or cardiomyopathy. Tension is signaled through the integrin adhesion complexes found at muscle insertions and costameres but the relative importance of signalling during cardiomyocyte growth versus remodelling has not been assessed. Employing the Drosophila cardiomyocyte as a genetically amenable model, we depleted the levels of Talin, a central component of the integrin adhesion complex, at different stages of heart growth and remodeling...
2015: PloS One
https://www.readbyqxmd.com/read/25801590/a-correlation-of-reactive-oxygen-species-accumulation-by-depletion-of-superoxide-dismutases-with-age-dependent-impairment-in-the-nervous-system-and-muscles-of-drosophila-adults
#10
Saori Oka, Jun Hirai, Takashi Yasukawa, Yasuyuki Nakahara, Yoshihiro H Inoue
The theory that accumulation of reactive oxygen species (ROS) in internal organs is a major promoter of aging has been considered negatively. However, it is still controversial whether overexpression of superoxide dismutases (SODs), which remove ROS, extends the lifespan in Drosophila adults. We examined whether ROS accumulation by depletion of Cu/Zn-SOD (SOD1) or Mn-SOD (SOD2) influenced age-related impairment of the nervous system and muscles in Drosophila. We confirmed the efficient depletion of Sod1 and Sod2 through RNAi and ROS accumulation by monitoring of ROS-inducible gene expression...
August 2015: Biogerontology
https://www.readbyqxmd.com/read/25532219/the-rna-binding-protein-arrest-bruno-regulates-alternative-splicing-to-enable-myofibril-maturation-in-drosophila-flight-muscle
#11
Maria L Spletter, Christiane Barz, Assa Yeroslaviz, Cornelia Schönbauer, Irene R S Ferreira, Mihail Sarov, Daniel Gerlach, Alexander Stark, Bianca H Habermann, Frank Schnorrer
In Drosophila, fibrillar flight muscles (IFMs) enable flight, while tubular muscles mediate other body movements. Here, we use RNA-sequencing and isoform-specific reporters to show that spalt major (salm) determines fibrillar muscle physiology by regulating transcription and alternative splicing of a large set of sarcomeric proteins. We identify the RNA-binding protein Arrest (Aret, Bruno) as downstream of salm. Aret shuttles between the cytoplasm and nuclei and is essential for myofibril maturation and sarcomere growth of IFMs...
February 2015: EMBO Reports
https://www.readbyqxmd.com/read/25189233/roles-of-the-troponin-isoforms-during-indirect-flight-muscle-development-in-drosophila
#12
Salam Herojeet Singh, Prabodh Kumar, Nallur B Ramachandra, Upendra Nongthomba
Troponin proteins in cooperative interaction with tropomyosin are responsible for controlling the contraction of the striated muscles in response to changes in the intracellular calcium concentration. Contractility of the muscle is determined by the constituent protein isoforms, and the isoforms can switch over from one form to another depending on physiological demands and pathological conditions. In Drosophila, amajority of themyofibrillar proteins in the indirect flight muscles (IFMs) undergo post-transcriptional and post-translational isoform changes during pupal to adult metamorphosis to meet the high energy and mechanical demands of flight...
August 2014: Journal of Genetics
https://www.readbyqxmd.com/read/25125178/smyd-proteins-key-regulators-in-skeletal-and-cardiac-muscle-development-and-function
#13
REVIEW
Shao Jun Du, Xungang Tan, Jianshe Zhang
Muscle fibers are composed of myofibrils, one of the most highly ordered macromolecular assemblies in cells. Recent studies demonstrate that members of the Smyd family play critical roles in myofibril assembly of skeletal and cardiac muscle during development. The Smyd family consists of five members including Smyd1, Smyd2, Smyd3, Smyd4, and Smyd5. They share two highly conserved structural and functional domains, namely the SET and MYND domains involved in lysine methylation and protein-protein interaction, respectively...
September 2014: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/25113030/the-nebulin-repeat-protein-lasp-regulates-i-band-architecture-and-filament-spacing-in-myofibrils
#14
Isabelle Fernandes, Frieder Schöck
Mutations in nebulin, a giant muscle protein with 185 actin-binding nebulin repeats, are the major cause of nemaline myopathy in humans. Nebulin sets actin thin filament length in sarcomeres, potentially by stabilizing thin filaments in the I-band, where nebulin and thin filaments coalign. However, the precise role of nebulin in setting thin filament length and its other functions in regulating power output are unknown. Here, we show that Lasp, the only member of the nebulin family in Drosophila melanogaster, acts at two distinct sites in the sarcomere and controls thin filament length with just two nebulin repeats...
August 18, 2014: Journal of Cell Biology
https://www.readbyqxmd.com/read/24794494/the-conserved-transmembrane-proteoglycan-perdido-kon-tiki-is-essential-for-myofibrillogenesis-and-sarcomeric-structure-in-drosophila
#15
Juan J Pérez-Moreno, Marcus Bischoff, Maria D Martín-Bermudo, Beatriz Estrada
Muscle differentiation requires the assembly of high-order structures called myofibrils, composed of sarcomeres. Even though the molecular organization of sarcomeres is well known, the mechanisms underlying myofibrillogenesis are poorly understood. It has been proposed that integrin-dependent adhesion nucleates myofibrils at the periphery of the muscle cell to sustain sarcomere assembly. Here, we report a role for the gene perdido (perd, also known as kon-tiki, a transmembrane chondroitin proteoglycan) in myofibrillogenesis...
July 15, 2014: Journal of Cell Science
https://www.readbyqxmd.com/read/24631244/tension-and-force-resistant-attachment-are-essential-for-myofibrillogenesis-in-drosophila-flight-muscle
#16
Manuela Weitkunat, Aynur Kaya-Çopur, Stephan W Grill, Frank Schnorrer
BACKGROUND: Higher animals generate an elaborate muscle-tendon network to perform their movements. To build a functional network, developing muscles must establish stable connections with tendons and assemble their contractile apparatuses. Current myofibril assembly models do not consider the impact of muscle-tendon attachment on myofibrillogenesis. However, if attachment and myofibrillogenesis are not properly coordinated, premature muscle contractions can destroy an unstable myotendinous system, leading to severe myopathies...
March 31, 2014: Current Biology: CB
https://www.readbyqxmd.com/read/24627474/mapping-interactions-between-myosin-relay-and-converter-domains-that-power-muscle-function
#17
William A Kronert, Girish C Melkani, Anju Melkani, Sanford I Bernstein
Intramolecular communication within myosin is essential for its function as motor, but the specific amino acid residue interactions required are unexplored within muscle cells. Using Drosophila melanogaster skeletal muscle myosin, we performed a novel in vivo molecular suppression analysis to define the importance of three relay loop amino acid residues (Ile(508), Asn(509), and Asp(511)) in communicating with converter domain residue Arg(759). We found that the N509K relay mutation suppressed defects in myosin ATPase, in vitro motility, myofibril stability, and muscle function associated with the R759E converter mutation...
May 2, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/24265259/the-role-of-pygopus-in-the-differentiation-of-intracardiac-valves-in-drosophila
#18
Min Tang, Wuzhou Yuan, Rolf Bodmer, Xiushan Wu, Karen Ocorr
Cardiac valves serve an important function; they support unidirectional blood flow and prevent blood regurgitation. Wnt signaling plays an important role in the formation of mouse cardiac valves and cardiac valve proliferation in Zebrafish, but identification of the specific signaling components involved has not been addressed systematically. Of the components involved in Wnt signal transduction, pygopus (pygo), first identified as a core component of Wnt signaling in Drosophila, has not yet to be investigated with respect to valve development and differentiation...
January 2014: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/24221941/a-drosophila-melanogaster-model-of-diastolic-dysfunction-and-cardiomyopathy-based-on-impaired-troponin-t-function
#19
Meera Cozhimuttam Viswanathan, Gaurav Kaushik, Adam J Engler, William Lehman, Anthony Cammarato
RATIONALE: Regulation of striated muscle contraction is achieved by Ca2+ -dependent steric modulation of myosin cross-bridge cycling on actin by the thin filament troponin-tropomyosin complex. Alterations in the complex can induce contractile dysregulation and disease. For example, mutations between or near residues 112 to 136 of cardiac troponin-T, the crucial TnT1 (N-terminal domain of troponin-T)-tropomyosin-binding region, cause cardiomyopathy. The Drosophila upheld(101) Glu/Lys amino acid substitution lies C-terminally adjacent to this phylogenetically conserved sequence...
January 17, 2014: Circulation Research
https://www.readbyqxmd.com/read/23830880/effect-of-myonuclear-number-and-mitochondrial-fusion-on-drosophila-indirect-flight-muscle-organization-and-size
#20
Mamta Rai, Upendra Nongthomba
Mechanisms involved in establishing the organization and numbers of fibres in a muscle are not completely understood. During Drosophila indirect flight muscle (IFM) formation, muscle growth is achieved by both incorporating hundreds of nuclei, and hypertrophy. As a result, IFMs provide a good model with which to understand the mechanisms that govern overall muscle organization and growth. We present a detailed analysis of the organization of dorsal longitudinal muscles (DLMs), a subset of the IFMs. We show that each DLM is similar to a vertebrate fascicle and consists of multiple muscle fibres...
October 15, 2013: Experimental Cell Research
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