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https://www.readbyqxmd.com/read/27932332/the-anterolateral-capsule-of-the-knee-behaves-like-a-sheet-of-fibrous-tissue
#1
Daniel Guenther, Amir A Rahnemai-Azar, Kevin M Bell, Sebastián Irarrázaval, Freddie H Fu, Volker Musahl, Richard E Debski
BACKGROUND: The function of the anterolateral capsule of the knee has not been clearly defined. However, the contribution of this region of the capsule to knee stability in comparison with other anterolateral structures can be determined by the relative force that each structure carries during loading of the knee. PURPOSE/HYPOTHESIS: The purpose of this study was to determine the forces in the anterolateral structures of the intact and anterior cruciate ligament (ACL)-deficient knee in response to an anterior tibial load and internal tibial torque...
December 8, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27930708/three-dimensional-construction-of-a-rabbit-anterior-corneal-replacement-for-lamellar-keratoplasty
#2
Kunpeng Pang, Liqun Du, Kai Zhang, Chenyang Dai, Chengqun Ju, Jing Zhu, Xinyi Wu
The aim of this study was to construct a rabbit anterior corneal replacement for transplantation using acellular porcine corneal matrix (APCM) and rabbit epithelial or stromal cells. APCM was prepared from fresh porcine cornea treated with 0.5% (wt./vol.) sodium dodecyl sulfate (SDS) solution. The expanded stromal cells were first injected into APCM parallel to its surface and were cultured in a shaking culture system for 7 days to obtain the stromal construct. Next, corneal epithelial cells were cultured on the stromal construct surface for another 7 days to obtain rabbit anterior corneal lamella...
2016: PloS One
https://www.readbyqxmd.com/read/27925461/in-it-for-the-long-haul-the-cytocompatibility-of-aged-graphene-oxide-and-its-degradation-products
#3
Brian D Holt, Anne M Arnold, Stefanie A Sydlik
Synthetic biomaterials are poised to transform medicine; however, current synthetic options have yet to ideally recapitulate the desirable properties of native tissue. Thus, the development of new synthetic biomaterials remains an active challenge. Due to its excellent properties, including electrical conductivity, water dispersibility, and capacity for functionalization, graphene oxide (GO) holds potential for myriads of applications, including biological devices. While many studies have evaluated the compatibility of freshly prepared GO, understanding the compatibility of GO as it ages in an aqueous environment is crucial for its safe implementation in long-term biological applications...
December 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27925007/viability-in-vitro-differentiation-and-molecular-characterization-of-equine-adipose-tissue-derived-mesenchymal-stem-cells-cryopreserved-in-serum-and-serum-free-medium
#4
B Merlo, S Pirondi, E Iacono, B Rossi, F Ricci, G Mari
BACKGROUND: The effect of freezing-thawing equine adipose tissue-derived mesenchymal stem cells (eATMSCs) have been poorly investigated. OBJECTIVE: This study is to test the influence of cryopreservation solution and temperature when adding the cryoprotectant for freezing eATMSCs, and to investigate the effects of cryopreservation on their stemness features. MATERIALS AND METHODS: Four freezing protocols were evaluated. Viability and proliferation ability of cryopreserved cells were investigated by MTT assay...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27924123/increased-oxidative-stress-lipid-peroxidation-and-protein-degradation-trigger-senescence-in-iris-versicolor-l-flowers
#5
Syed Sabhi Ahmad, Inayatullah Tahir
Dynamics in various physiological and biochemical aspects were studied during various stages (I-tight bud stage to VI-senescent stage) of flower development in Iris versicolor. Floral diameter, fresh & dry mass and water content increased during flower opening and decreased towards senescence. Senescence was found to be related to the increased lipid peroxidation which was reflected in the decreased membrane stability index towards senescence. This increase in the lipid peroxidation was probably initiated by increased lipoxygenase activity which shot up just prior to the increase in lipid peroxidation...
October 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27924070/modified-methods-for-isolation-of-pancreatic-stellate-cells-from-human-and-rodent-pancreas
#6
Liangtao Zhao, Baobao Cai, Zipeng Lu, Lei Tian, Song Guo, Pengfei Wu, Dong Qian, Qingcheng Xu, Kuirong Jiang, Yi Miao
Primary cultures of pancreatic stellate cells (PSCs) remain an important basis for in vitro study. However, effective methods for isolating abundant PSCs are currently lacking. We report on a novel approach to isolating PSCs from normal rat pancreases and human pancreatic ductal adenocarcinoma (PDAC) tissue. After anaesthesia and laparotomy of the rat, a blunt cannula was inserted into the pancreatic duct through the anti-mesentery side of the duodenum, and the pancreas was slowly infused with an enzyme solution until all lobules were fully dispersed...
November 2016: Journal of Biomedical Research
https://www.readbyqxmd.com/read/27922982/mir-21-is-overexpressed-in-hydatidiform-mole-tissues-and-promotes-proliferation-migration-and-invasion-in-choriocarcinoma-cells
#7
Ya-Xin Wang, Jiu-Ru Zhao, Yue-Ying Xu, Wei-Bin Wu, Hui-Juan Zhang
OBJECTIVE: The aims of this study were to make clear whether miR-21 was dysregulated in hydatidiform mole (HM) tissues and choriocarcinoma (CCA) cells, to elucidate whether aberrant miR-21 expression would affect the function of CCA cells, and to find out whether there was a relationship between miR-21 and AKT, PDCD4, and PTEN in CCA cells. METHODS: Fresh and formalin-fixed, paraffin-embedded trophoblastic tissues (normal first trimester placentas and HMs) were retrieved from the biobank in the International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University...
December 3, 2016: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/27920667/double-in-situ-hybridization-for-micrornas-and-mrnas-in-brain-tissues
#8
Atsushi Kasai, Sora Kakihara, Hiroki Miura, Ryo Okada, Atsuko Hayata-Takano, Keisuke Hazama, Misaki Niu, Norihito Shintani, Takanobu Nakazawa, Hitoshi Hashimoto
MicroRNAs (miRNAs) participate in a variety of functions in the brain. Understanding the in vivo localization of miRNAs is an important step for uncovering their roles in brain function. However, the in situ detection of low-abundance miRNAs in brain tissues remains difficult and requires extensive optimization of in situ hybridization (ISH) protocols in individual laboratories. Thus, detailed information regarding experimental conditions would serve as a useful reference for researchers in this field. Here, we investigated and summarized the effects of adjusting a series of critical steps, including tissue fixation, probe accessibility and hybridization stringency, to standardize the currently used miRNA ISH procedures...
2016: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/27917673/-first-attempts-in-the-introduction-of-cryopreservation-of-ovarian-tissues
#9
Péter Fancsovits, János Urbancsek, László Fónyad, Anna Sebestyén, Gézáné Csorba, Ádám Lehner, Zita Kaszás, János Rigó, Attila Bokor
INTRODUCTION: The oncological treatment may damage ovarian function. To prevent this, it is possible to cryopreserve the ovarian tissue, and to keep the samples for long-term storage. The frozen-thawed tissue could be retransplanted after chemo- or radiotherapy. AIM: The aim of our study was to examine the effect of cryopreservation on the viability of ovarian tissue. METHOD: We analyzed the survival of frozen-thawed donated ovarian tissues...
December 2016: Orvosi Hetilap
https://www.readbyqxmd.com/read/27913544/treatment-of-rare-factor-deficiencies-in-2016
#10
Flora Peyvandi, Marzia Menegatti
Rare bleeding disorders (RBDs) are a heterogeneous group of coagulation disorders characterized by fibrinogen, prothrombin, factors V, VII, X, XI, or XIII (FV, FVII, FX, FXI, or FXIII, respectively), and the combined factor V + VIII and vitamin K-dependent proteins deficiencies, representing roughly 5% of all bleeding disorders. They are usually transmitted as autosomal, recessive disorders, and the prevalence of the severe forms could range from 1 case in 500 000 for FVII up to 1 in 2-3 million for FXIII in the general population...
December 2, 2016: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/27911273/robust-detection-of-immune-transcripts-in-ffpe-samples-using-targeted-rna-sequencing
#11
Benjamin E Paluch, Sean T Glenn, Jeffrey M Conroy, Antonios Papanicolau-Sengos, Wiam Bshara, Angela R Omilian, Elizabeth Brese, Mary Nesline, Blake Burgher, Jonathan Andreas, Kunle Odunsi, Kevin Eng, Ji He, Maochun Qin, Mark Gardner, Lorenzo Galluzzi, Carl D Morrison
Current criteria for identifying cancer patients suitable for immunotherapy with immune checkpoint blockers (ICBs) are subjective and prone to misinterpretation, as they mainly rely on the visual assessment of CD274 (best known as PD-L1) expression levels by immunohistochemistry (IHC). To address this issue, we developed a RNA sequencing (RNAseq)-based approach that specifically measures the abundance of immune transcripts in formalin-fixed paraffin embedded (FFPE) specimens. Besides exhibiting superior sensitivity as compared to whole transcriptome RNAseq, our assay requires little starting material, implying that it is compatible with RNA degradation normally caused by formalin...
November 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/27911030/acellular-endocardium-as-a-novel-biomaterial-for-the-intima-of-tissue-engineered-small-caliber-vascular-grafts
#12
Feng Wang, Xin Guan, TianYi Wu, JianOu Qiao, ZhaoQing Han, JinLong Wu, XiaoWei Yu, QingJun You
We aimed to investigate whether acellular endocardium can be used as a useful biomaterial for the intima of engineered small-caliber vascular grafts. Fresh endocardium was harvested from the swine left atrium and was decellularized by digestion with the decellularization solution of Triton X-100 and SDS containing DNase I and RNase A. Surface morphological characteristics and Young's modulus were evaluated. To analyze the effect of mechanical characteristics on cell adhesion, the decellularized endocardium was stiffened with 2...
December 2016: Artificial Organs
https://www.readbyqxmd.com/read/27910016/applications-of-fluorescence-in-situ-hybridization-technology-in-malignancies
#13
Montakarn Tansatit
The molecular characterization of nonrandom recurrent cytogenetic abnormalities has identified numerous disease-related genes involved in hematologic and lymphoid malignancies. Cytogenetic analysis has become essential for disease diagnosis, classification, prognostic stratification, and treatment guidance. Fluorescence in situ hybridization (FISH) has greatly enhanced the field and enabled a more precise determination of the presence and frequency of genetic abnormalities. The advantages of FISH compared to standard cytogenetic analysis are that FISH can be used to identify genetic changes that are too small to be detected under a microscope, does not require cell culture, and can be applied directly on fresh or paraffin-embedded tissues for rapid evaluation of interphase nuclei...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27909613/the-novel-laparoscopic-training-3d-model-in-urology-with-surgical-anatomic-remarks-fresh-frozen-cadaveric-tissue
#14
REVIEW
Emre Huri, Mehmet Ezer, Eddie Chan
Laparoscopic surgery is routinely used to treat many urological conditions and it is the gold standard treatment option for many surgeries such as radical nephrectomy. Due to the difficulty of learning, laparoscopic training should start outside the operating room. Although it is a very different model of laparoscopic training; the aim of this review is to show the value of human cadaveric model for laparoscopic training and present our experience in this area. Fresh frozen cadaveric model in laparoscopic training, dry lab, cadaveric model, animal models and computer based simulators are the most commonly used models for laparoscopic training...
December 2016: Turkish Journal of Urology
https://www.readbyqxmd.com/read/27907194/preservation-of-multiple-mammalian-tissues-to-maximize-science-return-from-ground-based-and-spaceflight-experiments
#15
Sungshin Choi, Hami E Ray, San-Huei Lai, Joshua S Alwood, Ruth K Globus
BACKGROUND: Even with recent scientific advancements, challenges posed by limited resources and capabilities at the time of sample dissection continue to limit the collection of high quality tissues from experiments that can be conducted only infrequently and at high cost, such as in space. The resources and time it takes to harvest tissues post-euthanasia, and the methods and duration of long duration storage, potentially have negative impacts on sample quantity and quality, thereby limiting the scientific outcome that can be achieved...
2016: PloS One
https://www.readbyqxmd.com/read/27905446/genomic-evolution-and-chemoresistance-in-germ-cell-tumours
#16
Amaro Taylor-Weiner, Travis Zack, Elizabeth O'Donnell, Jennifer L Guerriero, Brandon Bernard, Anita Reddy, G Celine Han, Saud AlDubayan, Ali Amin-Mansour, Steven E Schumacher, Kevin Litchfield, Clare Turnbull, Stacey Gabriel, Rameen Beroukhim, Gad Getz, Scott L Carter, Michelle S Hirsch, Anthony Letai, Christopher Sweeney, Eliezer M Van Allen
Germ-cell tumours (GCTs) are derived from germ cells and occur most frequently in the testes. GCTs are histologically heterogeneous and distinctly curable with chemotherapy. Gains of chromosome arm 12p and aneuploidy are nearly universal in GCTs, but specific somatic genomic features driving tumour initiation, chemosensitivity and progression are incompletely characterized. Here, using clinical whole-exome and transcriptome sequencing of precursor, primary (testicular and mediastinal) and chemoresistant metastatic human GCTs, we show that the primary somatic feature of GCTs is highly recurrent chromosome arm level amplifications and reciprocal deletions (reciprocal loss of heterozygosity), variations that are significantly enriched in GCTs compared to 19 other cancer types...
November 30, 2016: Nature
https://www.readbyqxmd.com/read/27904780/low-expression-of-rbms3-and-sfrp1-are-associated-with-poor-prognosis-in-patients-with-gastric-cancer
#17
Tao Zhang, Youliang Wu, Zheng Fang, Qiang Yan, Shangxin Zhang, Ruochuan Sun, Junaid Khaliq, Yongxiang Li
RNA binding motif, single stranded interacting protein 3 (RBMS3) has been reported as a tumor suppressor gene (TSG) in some squamous carcinoma. However, its expression levels and clinical significance in gastric cancer (GC) remains unclear. Secreted frizzled-related protein 1 (SFRP1) plays a role of tumor suppressor in many cancers by inhibiting Wnt/β-catenin pathway. Nevertheless, its expression levels and clinical significance in GC are in dispute. In this study, quantitative real-time PCR and Western Blot were used to measure the mRNA and protein level of RBMS3 and SFRP1 in 23 fresh GC and corresponding normal tissues...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27904225/the-inferior-medial-genicular-artery-and-its-vascularization-of-the-pes-anserinus-superficialis-a-cadaveric-study
#18
Lena Hirtler, Manuel Ederer, Mike Faber, Patrick Weninger
BACKGROUND: A common method for reconstruction of the anterior cruciate ligament (ACL) is it's replacement by a free avascular graft, using the gracilis and/or semitendinosus tendons. These grafts pass a vulnerable phase in the ligamentization-process during the 1(st) year after reconstruction. The aims of this study were first to evaluate the vascularization of the pes anserinus superficialis (PAS) by the inferior medial genicular artery (IMGA) and second to develop a pedunculated surgical technique for ACL reconstruction, to preserve a maximal amount of natural vascularization of the tendons inserting at the PAS...
November 2016: Indian Journal of Orthopaedics
https://www.readbyqxmd.com/read/27903244/cryopreservation-of-dermal-fibroblasts-and-keratinocytes-in-hydroxyethyl-starch-based-cryoprotectants
#19
Yahaira Naaldijk, Adiv A Johnson, Annett Friedrich-Stöckigt, Alexandra Stolzing
BACKGROUND: Preservation of human skin fibroblasts and keratinocytes is essential for the creation of skin tissue banks. For successful cryopreservation of cells, selection of an appropriate cryoprotectant agent (CPA) is imperative. The aim of this study was to identify CPAs that minimize toxic effects and allow for the preservation of human fibroblasts and keratinocytes in suspension and in monolayers. RESULTS: We cryopreserved human fibroblasts and keratinocytes with different CPAs and compared them to fresh, unfrozen cells...
December 1, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27902480/down-regulation-of-rna-binding-motif-single-stranded-interacting-protein-3-along-with-up-regulation-of-nuclear-hif1a-correlates-with-poor-prognosis-in-patients-with-gastric-cancer
#20
Youliang Wu, Dapeng Yun, Yingjie Zhao, Yuqi Wang, Ruochuan Sun, Qiang Yan, Shangxin Zhang, Mingdian Lu, Zhen Zhang, Daru Lu, Yongxiang Li
Frequent loss of multiple regions in short arm of chromosome 3 is found in various tumors including gastric cancer (GC). RNA binding motif, single-stranded interacting protein 3 (RBMS3) is a tumor suppressor gene located in this region and mediates cancer angiogenesis. However, the role of RBMS3 in GC remains unclear.To evaluate whether RBMS3, together with HIF1A, another key regulator of angiogenesis, predicts GC prognosis, the levels of RBMS3 and HIF1A were first examined by quantitative PCR (qPCR) and western blot from 27 fresh frozen GC and paired normal gastric tissues and then tested by immunohistochemistry (IHC) from 191 GC and 46 normal controls...
November 25, 2016: Oncotarget
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