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https://www.readbyqxmd.com/read/28716864/alpha1-antitrypsin-binds-hemin-and-prevents-oxidative-activation-of-human-neutrophils-putative-pathophysiological-significance
#1
Sabina Janciauskiene, Srinu Tumpara, Malgorzata Wiese, Sabine Wrenger, Vijith Vijayan, Faikah Gueler, Rongjun Chen, Kukuh Madyaningrana, Ravi Mahadeva, Tobias Welte, Stephan Immenschuh, Joanna Chorostowska-Wynimko
Heme is a ubiquitous compound of human tissues, and it is involved in cellular physiology and metabolism. Once released from the cell, free heme oxidizes to the ferric state (hemin). High levels of hemin can cause oxidative stress and inflammation if not neutralized immediately by specialized scavenger proteins. Human alpha1-antitrypsin (A1AT), an acute-phase glycoprotein and important inhibitor of neutrophil proteases, is also a hemin-binding protein. A short-term exposure of freshly isolated human blood neutrophils to 4 µM hemin results in cell spreading, surface expression of filament protein, vimentin, free radical production, expression of heme oxygenase-1 (HO-1), release of IL-8, and enhanced neutrophil adhesion to human endothelial cells...
July 17, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28716599/maintaining-viability-and-characteristics-of-cholangiocarcinoma-tissue-by-vitrification-based-cryopreservation
#2
Min Zeng, Qiu-Rui Yang, Gong-Bo Fu, Yuan Zhang, Xu Zhou, Wei-Jian Huang, Hong-Dan Zhang, Wei-Jian Li, Zhen-Yu Wang, He-Xin Yan, Bo Zhai
Tumor tissue has great clinical and scientific value which relies highly on the proper preservation of primary materials. Conventional tumor tissue cryopreservation using slow-freezing method has yielded limited success, leading to significant cell loss and morphological damage. Here we report a standardized vitrification-based cryopreservation method, by which we have successfully vitrified and warmed 35 intrahepatic cholangiocarcinoma (ICC) tissues with up to 80% viability of the fresh tumor tissues. Cryopreserved ICC tissue could generate patient-derived xenografts (PDXs) with take rates of 68...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28716597/cells-isolated-from-cryopreserved-dental-follicle-display-similar-characteristics-to-cryopreserved-dental-follicle-cells
#3
Hefeng Yang, Jie Li, JingJing Sun, Weihua Guo, Hui Li, Jinglong Chen, Yu Hu, Weidong Tian, Song Li
Dental follicle tissue is a promising resource of mesenchymal stem cells for cytotherapeutic approaches and tissue engineering applications. There are two procedures for banking of human dental follicle stem cells have been reported. Conventional method requires cell isolation, expansion and immediate cryopreservation. Whereas dental follicle stem cells can be isolated from cryopreserved dental follicle fragments. The aim of this study was to compare the characteristics of dental follicle cells isolated from cryopreserved fragments (DFCs-CF) with dental follicle cells recovered from cryopreserved cells (DFCs-CC)...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28716099/xenoantigenicity-of-porcine-decellularized-valves
#4
Meghana R K Helder, Nicholas J Stoyles, Brandon J Tefft, Ryan S Hennessy, Rebecca R C Hennessy, Roy Dyer, Tyra Witt, Robert D Simari, Amir Lerman
BACKGROUND: The xenoantigenicity of porcine bioprosthetic valves is implicated as an etiology leading to calcification and subsequent valve failure. Decellularization of porcine valves theoretically could erase the antigenicity of the tissue leading to more durable prosthetic valves, but the effectiveness of decellularization protocols in regard to completely removing antigens has yet to be verified. Our hypothesis was that decellularization would remove the more abundant α-gal antigens but not remove all the non α-gal antigens, which could mount a response...
July 17, 2017: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/28715607/variation-in-the-dielectric-properties-of-freshly-excised-colorectal-cancerous-tissues-at-different-tumor-stages
#5
Zhou Li, Weiwei Wang, Zhai Cai, Shuai Han, Shiming Lin, Linyun He, Miaoliang Chen, Dongyue Pan, Guanhua Deng, Song Duan, Sherman Xuegang Xin
The study was designed to identify differences in the dielectric properties of ex vivo colorectal cancerous tissues at different tumor stages. To date, 130 freshly excised colorectal cancerous specimens underwent measurement of both relative permittivity and conductivity on the serosal and mucosal surfaces of the carcinoma nidus, and the mucosa of the surgical resection margin ranging from 50 to 500 MHz at the Larmor frequencies. Tumor node metastasis staging was determined according to pathological reports for each patient...
July 17, 2017: Bioelectromagnetics
https://www.readbyqxmd.com/read/28715124/chemical-imaging-of-fresh-vascular-smooth-muscle-cell-response-by-epi-detected-stimulated-raman-scattering
#6
Delong Zhang, Wei Chen, Huan Chen, Han-Qing Yu, Ghassan Kassab, Ji-Xin Cheng
An understanding of deformation of cardiovascular tissue under hemodynamic load is crucial for understanding the health and disease of blood vessels. In the present work, an epi-detected stimulated Raman scattering (epi-SRS) imaging platform was designed for in situ functional imaging of vascular smooth muscle cells (VMSCs) in fresh coronary arteries. For the first time, the pressure-induced morphological deformation of fresh VSMCs was imaged with no fixation and in a label free manner. The relation between the loading pressure and the morphological parameters, including angle and length of the VSMCs, were apparent...
July 17, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28714966/nanoscale-imaging-of-clinical-specimens-using-pathology-optimized-expansion-microscopy
#7
Yongxin Zhao, Octavian Bucur, Humayun Irshad, Fei Chen, Astrid Weins, Andreea L Stancu, Eun-Young Oh, Marcello DiStasio, Vanda Torous, Benjamin Glass, Isaac E Stillman, Stuart J Schnitt, Andrew H Beck, Edward S Boyden
Expansion microscopy (ExM), a method for improving the resolution of light microscopy by physically expanding a specimen, has not been applied to clinical tissue samples. Here we report a clinically optimized form of ExM that supports nanoscale imaging of human tissue specimens that have been fixed with formalin, embedded in paraffin, stained with hematoxylin and eosin, and/or fresh frozen. The method, which we call expansion pathology (ExPath), converts clinical samples into an ExM-compatible state, then applies an ExM protocol with protein anchoring and mechanical homogenization steps optimized for clinical samples...
July 17, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28708217/evaluation-of-calcium-ca2-and-hydroxide-oh-ion-diffusion-rates-of-indirect-pulp-capping-materials
#8
Merve Kurun Aksoy, Firdevs Tulga Oz, Kaan Orhan
INTRODUCTION: The aim of this study was to evaluate and compare the calcium (Ca2+) and hydroxide (OH-) ion release of 4 artificially produced pulp capping materials (MTA, Biodentin, TheraCal LC, Calsimol) used for indirect pulp capping treatment. METHODS: In total, 70 freshly extracted human third molar teeth were used for the study. Cavities of extracted teeth were prepared by round burs. The remaining dentin thickness (1 ± 0.3 mm) tissue was measured by a micrometer and cone beam computerized tomography...
July 8, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28706339/expansion-of-bone-marrow-derived-human-mesenchymal-stem-stromal-cells-hmscs-using-a-two-phase-liquid-liquid-system
#9
Mariana P Hanga, Halina Murasiewicz, Andrzej W Pacek, Alvin W Nienow, Karen Coopman, Christopher J Hewitt
BACKGROUND: Human mesenchymal stem/stromal cells (hMSCs) are at the forefront of regenerative medicine applications due to their relatively easy isolation and availability in adults, potential to differentiate and to secrete a range of trophic factors that could determine specialised tissue regeneration. To date, hMSCs have been successfully cultured in vitro on substrates such as polystyrene dishes (TCPS) or microcarriers. However, hMSC sub-cultivation and harvest typically employs proteolytic enzymes that act by cleaving important cell membrane proteins resulting in long-term cell damage...
July 2017: Journal of Chemical Technology and Biotechnology
https://www.readbyqxmd.com/read/28704486/a-common-bottlenose-dolphin-tursiops-truncatus-prey-handling-technique-for-marine-catfish-ariidae-in-the-northern-gulf-of-mexico
#10
Errol I Ronje, Kevin P Barry, Carrie Sinclair, Mark A Grace, Nélio Barros, Jason Allen, Brian Balmer, Anna Panike, Christina Toms, Keith D Mullin, Randall S Wells
Few accounts describe predator-prey interactions between common bottlenose dolphins (Tursiops truncatus Montagu 1821) and marine catfish (Ariopsis felis Linnaeus 1766, Bagre marinus Mitchill 1815). Over the course of 50,167 sightings of bottlenose dolphin groups in Mississippi Sound and along the Florida coast of the Gulf of Mexico, severed catfish heads were found floating and exhibiting movements at the surface in close proximity to 13 dolphin groups that demonstrated feeding behavior. These observations prompted a multi-disciplinary approach to study the predator-prey relationship between bottlenose dolphins and marine catfish...
2017: PloS One
https://www.readbyqxmd.com/read/28701069/current-success-and-efficiency-of-autologous-ovarian-transplantation-a-meta-analysis
#11
Fernanda Pacheco, Kutluk Oktay
CONTEXT: Ovarian cryopreservation followed by autotransplantation is still considered an experimental strategy for fertility preservation (FP) mainly because the success rates are unknown. OBJECTIVE: To determine cohort epidemiologic characteristics and success rates of autologous ovarian tissue transplantation (OTT) with previously cryopreserved tissue. MATERIALS AND METHODS: Literature review from 1999 to October 1, 2016. Additional cases were retrieved from meeting abstracts and own database...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28700694/a-robust-and-efficient%C3%A2-method-for-the-extraction-of-plant-extracellular-surface-lipids-as-applied-to-the-analysis-of-silks-and-seedling-leaves-of-maize
#12
Derek M Loneman, Layton Peddicord, Amani Al-Rashid, Basil J Nikolau, Nick Lauter, Marna D Yandeau-Nelson
Aerial plant organs possess a diverse array of extracellular surface lipids, including both non-polar and amphipathic constituents that collectively provide a primary line of defense against environmental stressors. Extracellular surface lipids on the stigmatic silks of maize are composed primarily of saturated and unsaturated linear hydrocarbons, as well as fatty acids, and aldehydes. To efficiently extract lipids of differing polarities from maize silks, five solvent systems (hexanes; hexanes:diethyl ether (95:5); hexanes:diethyl ether (90:10); chloroform:hexanes (1:1) and chloroform) were tested by immersing fresh silks in solvent for different extraction times...
2017: PloS One
https://www.readbyqxmd.com/read/28699896/eb1-protein-alteration-characterizes-sporadic-but-not-ulcerative-colitis-associated-colorectal-cancer
#13
Timo Gemoll, Sophie L Kollbeck, Karl F Karstens, Gia G Hò, Sonja Hartwig, Sarah Strohkamp, Katharina Schillo, Christoph Thorns, Martina Oberländer, Kathrin Kalies, Stefan Lehr, Jens K Habermann
BACKGROUND: While carcinogenesis in Sporadic Colorectal Cancer (SCC) has been thoroughly studied, less is known about Ulcerative Colitis associated Colorectal Cancer (UCC). This study aimed to identify and validate differentially expressed proteins between clinical samples of SCC and UCC to elucidate new insights of UCC/SCC carcinogenesis and progression. RESULTS: Multiplex-fluorescence two-dimensional gel electrophoresis (2-D DIGE) and mass spectrometry identified 67 proteoforms representing 43 distinct proteins...
July 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28698418/-reconstruction-of-anterior-mitral-leaflet-using-fresh-autologous-pericardial-patch-for-active-infective-endocarditis-report-of-a-case
#14
Hideaki Kanda, Tamahiro Kinjo, Yosuke Hisashi
Several reports have described that the prognosis of patients with mitral valve regurgitation due to active infective endocarditis (IE) is better after mitral valve plasty (MVP) than replacement (MVR). However, extensive destruction of valve tissue might cause difficulties with MVP. We repaired a widely-affected anterior mitral leaflet (AML) using an autologous pericardial patch. A 44-year-old woman with mitral regurgitation presented with prolonged fever and backache. We made a diagnosis of active IE accompanied by mitral valve regurgitation...
July 2017: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/28697743/integrative-analysis-of-copy-number-and-gene-expression-in-breast-cancer-using-formalin-fixed-paraffin-embedded-core-biopsy-tissue-a-feasibility-study
#15
Mahesh Iddawela, Oscar Rueda, Jenny Eremin, Oleg Eremin, Jed Cowley, Helena M Earl, Carlos Caldas
BACKGROUND: An absence of reliable molecular markers has hampered individualised breast cancer treatments, and a major limitation for translational research is the lack of fresh tissue. There are, however, abundant banks of formalin-fixed paraffin-embedded (FFPE) tissue. This study evaluated two platforms available for the analysis of DNA copy number and gene expression using FFPE samples. METHODS: The cDNA-mediated annealing, selection, extension, and ligation assay (DASL™) has been developed for gene expression analysis and the Molecular Inversion Probes assay (Oncoscan™), were used for copy number analysis using FFPE tissues...
July 11, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28696914/micro-brushing-based-technique-to-gain-fresh-urothelial-cells-for-gene-expression-analysis
#16
Christoph Kuhn, Marie-Louise Lehmann, Alexander Kress, Michael C Truß, Matthias Hermes, Meinolf Blaszkewicz, Jan G Hengstler, Klaus Golka
The gold standard of saving fresh tissue in liquid nitrogen has some serious disadvantages in that this process is not available in daily medical routine practices even in many tumor centers. Our approach of a new minimally invasive technique is obtaining urothelial cells via micro-brushing the urinary bladder on the occasion of urological routine methods such as transurethral resection (TUR). Urothelial cells were obtained from 25 patients via two different micro-brushes from tumor tissue and from macroscopically healthy tissue during TUR...
July 11, 2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28694032/biodegradable-brain-penetrating-dna-nanocomplexes-and-their-use-to-treat-malignant-brain-tumors
#17
Panagiotis Mastorakos, Clark Zhang, Eric Song, Young Eun Kim, Hee Won Park, Sneha Berry, Won Kyu Choi, Justin Hanes, Jung Soo Suk
The discovery of powerful genetic targets has spurred clinical development of gene therapy approaches to treat patients with malignant brain tumors. However, lack of success in the clinic has been attributed to the inability of conventional gene vectors to achieve gene transfer throughout highly disseminated primary brain tumors. Here, we demonstrate ex vivo that small nanocomplexes composed of DNA condensed by a blend of biodegradable polymer, poly(β-amino ester) (PBAE), with PBAE conjugated with 5kDa polyethylene glycol (PEG) molecules (PBAE-PEG) rapidly penetrate healthy brain parenchyma and orthotopic brain tumor tissues in rats...
July 7, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28690805/evaluating-hybridization-capture-with-rad-probes-as-a-tool-for-museum-genomics-with-historical-bird-specimens
#18
Ethan B Linck, Zachary R Hanna, Anna Sellas, John P Dumbacher
Laboratory techniques for high-throughput sequencing have enhanced our ability to generate DNA sequence data from millions of natural history specimens collected prior to the molecular era, but remain poorly tested at shallower evolutionary time scales. Hybridization capture using restriction site-associated DNA probes (hyRAD) is a recently developed method for population genomics with museum specimens. The hyRAD method employs fragments produced in a restriction site-associated double digestion as the basis for probes that capture orthologous loci in samples of interest...
July 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28690627/isothiocyanates-nitriles-and-epithionitriles-from-glucosinolates-are-affected-by-genotype-and-developmental-stage-in-brassica-oleracea-varieties
#19
Franziska S Hanschen, Monika Schreiner
Vegetables of the Brassica oleracea group, such as broccoli, cauliflower, and cabbage, play an important role for glucosinolate consumption in the human diet. Upon maceration of the vegetable tissue, glucosinolates are degraded enzymatically to form volatile isothiocyanates, nitriles, and epithionitriles. However, only the uptake of isothiocyanates is linked to the cancer-preventive effects. Thus, it is of great interest to evaluate especially the isothiocyanate formation. Here, we studied the formation of glucosinolates and their respective hydrolysis products in sprouts and fully developed vegetable heads of different genotypes of the five B...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28687883/combination-of-maldi-msi-and-cassette-dosing-for-evaluation-of-drug-distribution-in-human-skin-explant
#20
Isabella S Sørensen, Christian Janfelt, Mette Marie B Nielsen, Rasmus W Mortensen, Nina Ø Knudsen, André H Eriksson, Anders J Pedersen, Kim T Nielsen
Study of skin penetration and distribution of the drug compounds in the skin is a major challenge in the development of topical drug products for treatment of skin diseases. It is crucial to have fast and efficacious screening methods which can provide information concerning the skin penetration and the distribution of the drug molecules in the region of the target. Mass spectrometry imaging (MSI) such as matrix-assisted laser desorption/ionization (MALDI)-MSI offers the opportunity to analyze the drug distribution at micrometer scale, but is a low throughput technique...
July 7, 2017: Analytical and Bioanalytical Chemistry
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