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Kwang Il Lee, Merissa Olmer, Jihye Baek, Darryl D D'Lima, Martin K Lotz
The aim of this study was to examine the potential of platelet-derived growth factor (PDGF)-coated decellularized meniscus scaffold in mediating integrative healing of meniscus tears by inducing endogenous cell migration. Fresh bovine meniscus was chemically decellularized and covalently conjugated with heparin and PDGF-BB. In vitro PDGF release kinetics was measured. The scaffold was transplanted into experimental tears in avascular bovine meniscus explants and cultured for 2 and 4 weeks. The number migrating and proliferating cells at the borderline between the scaffold and injured explant and PDGF receptor-β (PDGFR β) expressing cells were counted...
June 13, 2018: Acta Biomaterialia
Danielle C Brito, Sheyla F S Domingues, Ana Paula R Rodrigues, José R Figueiredo, Regiane R Santos, Júlio C Pieczarka
We aimed to evaluate the effect of three extracellular cryoprotectants on the morphology of vitrified feline preantral follicles. Feline ovarian fragments (0.5 × 2 × 2 mm) collected from five domestic adult cats subjected to ovariohysterectomy for routine castration were vitrified with ethylene glycol (EG) 40% combined or not with sucrose (0.1 or 0.5 M), trehalose (0.1 or 0.5 M), or raffinose (0.1 M). After vitrification using the solid-surface method and warming of the tissues, cryoprotectants were washed out of the ovarian tissues, which were fixed for histological analysis...
June 13, 2018: Cryobiology
Fedia Souilem, Asma El Ayeb, Brahim Djlassi, Olfa Ayari, Wiem Chiboub, Faten Arbi, Roberta Ascrizzi, Guido Flamini, Fethia Harzallah-Skhiri
This is the first study investigating the chemical composition of essential oils (EOs) isolated from different tissues of Carissa macrocarpa (Eckl.)A.DC, their antimicrobial activity and the anatomical characters of the aerial organs and the fruits. The main EO components were pentadecanal and tetradecan-1-ol (31.9 and 16.5% in fresh leaf EO, resp.), (E)-nerolidol and caryophyllene oxide (27.3 and 15.0% in fruit EO, resp.), linalool and hexahydrofarnesyl acetone (30.9 and 24.9% in stem EO, resp.), benzyl benzoate (24...
June 15, 2018: Chemistry & Biodiversity
Tatsunori Shimoi, Akinobu Hamada, Marifu Yamagishi, Mitsuharu Hirai, Masayuki Yoshida, Tadaaki Nishikawa, Kazuki Sudo, Akihiko Shimomura, Emi NoguchI, Mayu Yunokawa, Kan Yonemori, Chikako Shimizu, Takayuki Kinoshita, Takahiro Fukuda, Yasuhiro Fujiwara, Kenji Tamura
PIK3CA mutations are common activating mutations associated with breast cancer (occurring in 20-30% of all cases) and are potent predictive markers for responses to PI3K inhibitors. Thus, it is important to develop sensitive methods to detect these mutations. We established a novel detection method using a quenching probe (QP) system to identify PIK3CA mutations, using DNA from 309 breast cancer tissues. In a developmental cohort, we determined the optimal detection threshold of the QP system with human tumor DNA from 119 freshly frozen tumor samples...
June 15, 2018: Cancer Science
Mingsheng Guo, Wei Yue, David C Samuels, Hui Yu, Jing He, Ying-Yong Zhao, Yan Guo
Genotyping arrays characterize genome-wide SNPs for a study cohort and were the primary technology behind genome wide association studies over the last decade. The Cancer Genome Atlas (TCGA) is one of the largest cancer consortium studies, and it collected genotyping data for all of its participants. Using TCGA SNP data genotyped using the Affymetrix 6.0 SNP array from 12,064 samples, we conducted a comprehensive comparisons across DNA sources (tumor tissue, normal tissue, and blood) and sample storage protocols (formalin-fixed paraffin-embedded (FFPE) vs...
June 11, 2018: Genomics
Ye Xia, Amna Amna, Stephen Obol Opiyo
Plants have a diverse endophytic microbiome that is functionally important for their growth, development, and health. In this study, the diversity and specificity of culturable endophytic fungal communities were explored in one of the most important biofuel crops, switchgrass plants (Panicum virgatum L.), which have been cultivated on a reclaimed coal-mining site for more than 20 years. The endophytic fungi were isolated from the surface-sterilized shoot (leaf and stem), root, and seed tissues of switchgrass plants and then cultured for identification...
2018: PloS One
Wei Zhao, Xingwang Kuai, Xueyi Zhou, Lizhou Jia, Jinsong Wang, Xiaobing Yang, Zhidan Tian, Xiaolei Wang, Qian Lv, Bin Wang, Youcai Zhao, Wenbin Huang
Trop2 is considered to have an important function in tumor metastasis and the promotion of epithelial‑mesenchymal transition (EMT). E‑cadherin is a crucial factor in intercellular adhesion and EMT transformation. In the present study, we detected the expression of Trop2 and E‑cadherin in breast cancer (BC) to better define their prognostic value. The mRNA expression levels of these two genes in 20 cases of fresh BC tissues were detected by quantitative real‑time polymerase chain reaction (qRT‑PCR)...
June 14, 2018: Oncology Reports
Laura Pinton, Samantha Solito, Elena Masetto, Marina Vettore, Stefania Canè, Alessandro Della Puppa, Susanna Mandruzzato
Meningiomas WHO grade I and II are common intracranial tumors in adults that normally display a benign outcome, but are characterized by a great clinical heterogeneity and frequent recurrence of the disease. Although the presence of an immune cell infiltrate has been documented in these tumors, a clear phenotypical and functional characterization of the immune web is missing. Here, we performed an extensive immunophenotyping of peripheral blood and fresh tumor tissue at surgery by multiparametric flow cytometry in 34 meningioma patients, along with immunosuppressive activity of sorted cells of myeloid origin...
2018: Oncoimmunology
Pei Xiao, Xinxin Long, Lijie Zhang, Yingnan Ye, Jincheng Guo, Pengpeng Liu, Rui Zhang, Junya Ning, Wenwen Yu, Feng Wei, Jinpu Yu
We previously demonstrated that neurotensin (NTS) induces local inflammation and promotes tumor invasion in hepatocellular carcinoma (HCC). However, the underlying molecular mechanisms are not clear. In this study, positive correlations between NTS and interleukin (IL)-8 were identified at both the mRNA and protein levels in 71 fresh HCC tissues and 100 paraffin-embedded HCC tissues. Furthermore, significant correlations were determined among the co-expression of NTS and IL-8, infiltration of inflammatory cells and enhanced epithelial-mesenchymal transition (EMT) of HCC cells...
2018: Oncoimmunology
Keiichi Hatakeyama, Takeshi Nagashima, Kenichi Urakami, Keiichi Ohshima, Masakuni Serizawa, Sumiko Ohnami, Yuji Shimoda, Shumpei Ohnami, Koji Maruyama, Akane Naruoka, Yasuto Akiyama, Masatoshi Kusuhara, Tohru Mochizuki, Ken Yamaguchi
Tumor mutational burden (TMB) is an emerging characteristic in cancer and has been associated with microsatellite instability, defective DNA replication/repair, and response to PD-1 and PD-L1 blockade immunotherapy. When estimating TMB, targeted panel sequencing is performed using a few hundred genes; however, a comparison of TMB results obtained with this platform and with whole exome sequencing (WES) has not been performed for various cancer types. In the present study, we compared TMB results using the above two platforms in 2,908 solid tumors that were obtained from Japanese patients...
2018: Biomedical Research
John Charles Rotondo, Alessandro Borghi, Rita Selvatici, Elisa Mazzoni, Ilaria Bononi, Monica Corazza, Jacqueline Kussini, Elena Montinari, Roberta Gafà, Mauro Tognon, Fernanda Martini
Importance: Molecular alterations in lichen sclerosus-associated vulvar squamous cell carcinoma (LS-VSCC) are largely unknown. Objective: To determine whether the retinoic acid receptor β (RARβ) tumor-suppressor gene is involved in the onset and/or progression of LS-VSCC. Design, Setting, and Participants: The case-control study, conducted at University-Hospital of Ferrara, Italy, included 20 LS-VSCC (mean [SD] age, 75 [3] years) and 20 cancer-associated vulvar LS (caVLS; mean [SD] age, 62 [11] years) formalin-fixed embedded tissue specimens, 20 cancer-free vulvar LS (cfVLS), and 20 normal skin fresh specimens from diagnostic biopsies and women surgically treated for nonmalignant skin lesions, respectively...
June 13, 2018: JAMA Dermatology
Bufang Xu, Mingjuan Zhou, Jingwen Wang, Dan Zhang, Feng Guo, Chenchen Si, Peter C K Leung, Aijun Zhang
STUDY QUESTION: Is allograft inflammatory factor-1 (AIF-1), a cytokine associated with inflammation and allograft rejection, aberrantly elevated in in vitro fertilization (IVF) cycles with gonadotropin-releasing hormone (GnRH) antagonist protocol with potential effects on endometrial receptivity? SUMMARY ANSWER: Our findings indicated AIF-1 is increased in IVF cycles with GnRH antagonist protocol and mediates greater TNF-α expression during implantation phase, which may be unfavorable for embryo implantation...
June 12, 2018: Human Reproduction
Roberto Crespi, Paolo Capparé, Giovanni Crespi, Giorgio Gastaldi, Georgios E Romanos, Enrico Gherlone
The aim of the present study was to consider the long-term midfacial mucosal outcome around final prosthetic restorations on dental implants placed and loaded immediately after tooth extractions. A total of 42 patients requiring tooth extractions were recruited, and 142 teeth were extracted. Based on the amount of keratinized mucosa (KM), implants were categorized into group A (KM ≥ 2 mm; n = 61) or group B (KM < 2 mm; n = 62). In both groups, all patients received temporary prosthetic restorations immediately after the surgical procedure...
June 12, 2018: International Journal of Periodontics & Restorative Dentistry
Zehava Ovadia-Blechman, Ashley Gritzman, Maya Shuvi, Benjamin Gavish, Vered Aharonson, Neta Rabin
BACKGROUND: The peripheral microcirculation supplies fresh blood to the small blood vessels, providing oxygen and nutrients to the tissues, removing waste, and maintaining normal homeostatic conditions. The goal of this study was to characterize the response of the peripheral microcirculation, in terms of blood flow and tissue oxygenation variables, to gravity-induced changes. METHODS: The study included 20 healthy volunteers and the experiment involved monitoring central and peripheral variables with the right hand positioned at different heights...
June 5, 2018: Clinical Biomechanics
Mark Seegobin, Anna Kisiala, Adam Noble, David Kaplan, Craig Brunetti, R J Neil Emery
Cytokinins (CKs) are a group of phytohormones essential to plant growth and development. The presence of these N6 -modified adenine derivatives has also been documented in other groups of organisms, including bacteria, fungi, and insects. Thus far, however, only a single CK, N6 -(Δ2 -isopentenyl) adenine-9-riboside (iPR), has been identified in mammals. In plants, the nucleotide form of isopentenyladenine [iPR (either mono-, di-, or tri-) phosphate (iPRP)] is the first form of CK synthesized, and it is further modified to produce other CK types...
June 12, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Omonigho Aisagbonhi, Abraham Birungi, Raymond Atwine, Paddy Behayo, Benon Ayebaziwe, Drucilla Roberts, Rosemary Tambouret
Objectives: The plasma-thrombin method is commonly used to make cell blocks from fine-needle aspiration (FNA) samples but requires centrifugation. We describe a modification to this method that does not require centrifugation for use in resource-limited settings. Methods: Pooled fresh plasma is aliquoted into 2-mL Eppendorf tubes and the FNA sample directly rinsed into the plasma. Two drops of reconstituted thrombin are added and gently mixed. A cell clot is transferred to a tissue bag, fixed in formalin, and processed...
June 9, 2018: American Journal of Clinical Pathology
Pushpendra Kumar, Shruti Sethi, R R Sharma, Manish Srivastav, Dinesh Singh, Eldho Varghese
Plum is a perishable fruit with a very short shelf life of 3-4 days. Several studies have suggested the possible use of edible coatings on maintaining quality and prolonging storage life of fresh horticultural produce. Hence, different edible coatings, namely, lac-based (2:3), Semperfresh™ (1:3) and Niprofresh® (1:5) were selected to observe their efficacy on quality retention of 'Santa Rosa' plums. The effects of these coatings on the physical, physiological and biochemical attributes were studied under cold storage conditions (2 ± 1 °C and 85-90% relative humidity)...
June 2018: Journal of Food Science and Technology
Nancy N Lovera, Laura Ramallo, Viviana O Salvadori
The effect of calcium impregnation on drip loss, colour, mechanical properties, sensory perception and freezing time on frozen-thawed papaya was studied, evaluating different freezing methods: cryogenic, tunnel and household freezer freezing. Osmotic dehydration as pre-treatment was also evaluated. Freezing in liquid nitrogen was considered an inappropriate method for papaya preservation due to cracking. Calcium impregnation and osmotic dehydration increased tissue firmness and decreased freezing time (freezing time for fresh, calcium impregnated and osmo-dehydrated fruit was 23, 17 and 5 min in a tunnel and 118, 83 and 60 min in a household freezer, respectively)...
June 2018: Journal of Food Science and Technology
Inna Solodeev, Benjamin Meilik, Ilan Volovitz, Meirav Sela, Sharon Manheim, Shai Yarkoni, Dov Zipori, Eyal Gur, Nir Shani
Fas-L is a TNF family member known to trigger cell death. It has recently become evident that Fas-L can transduce also non-apoptotic signals. Mesenchymal stem cells (MSCs) are multipotent cells that are derived from various adult tissues. Although MSCs from different tissues display common properties they also display tissue-specific characteristics. Previous works have demonstrated massive apoptosis following Fas-L treatment of bone marrow-derived MSCs both in vitro and following their administration in vivo...
June 11, 2018: Cell Death & Disease
Jia Luo, Hongkang Zhou, Libing Rao, Tianxiong Liu, Lian Liu, Yanliang Chen, Jianlong Wang, Tu Hu, Shiqi Liu, Dan Chen, Hui Wang, Leping Zeng, JuFang Huang
OBJECTIVES: We have explored a better method to preserve and store human medically amputated large size samples. The approach involved developing a special embalming solution and procedures for biopreservation and biostorage of a large-sized sample as a whole specimen rather than dissected small parts. Evaluation of the effect of our special embalming solution and procedures on whole human amputated extremities compared with excised small tissues was conducted. Histological and morphological techniques and elemental analyses were utilized to assess the effects of our new method using the special embalming solution...
June 11, 2018: Biopreservation and Biobanking
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