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https://www.readbyqxmd.com/read/29666023/the-influence-of-lens-care-systems-on-eyelid-tissue-changes-during-silicone-hydrogel-contact-lens-wear
#1
Michel Guillon, Cécile Maissa, Stéphanie Wong, Trisha Patel, Renée Garofalo
PURPOSE: To compare the effects of a hydrogen peroxide (H2 O2 )-based lens care solution and a polyhexamethylene biguanide (PHMB) multi-purpose solution on the eyelids when used with silicone hydrogel (SiHy) contact lenses. METHODS: A total of 74 symptomatic wearers of ACUVUE® OASYS® (senofilcon A; n = 39) or PureVision® (balafilcon A; n = 35) contact lenses were randomised 1:1 to either CLEAR CARE® Cleaning & Disinfecting Solution or renu® fresh™ multi-purpose solution (n = 37 each)...
April 14, 2018: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/29665503/zebrafish-extracellular-matrix-improves-neuronal-viability-and-network-formation-in-a-3-dimensional-culture
#2
Sung-Min Kim, Daniel Ward Long, Michael Wai Kok Tsang, Yadong Wang
Mammalian central nervous system (CNS) has limited capacity for regeneration. CNS injuries cause life-long debilitation and lead to $50 billion in healthcare costs in U.S. alone each year. Despite numerous efforts in the last few decades, CNS-related injuries remain as detrimental as they were 50 years ago. Some functional recovery can occur, but most regeneration are limited by an extracellular matrix (ECM) that actively inhibits axonal repair and promotes glial scarring. In most tissues, the ECM is an architectural foundation that plays an active role in supporting cellular development and regenerative response after injury...
April 6, 2018: Biomaterials
https://www.readbyqxmd.com/read/29665316/clinical-significance-of-tm4sf1-as-a-tumor-suppressor-gene-in-gastric-cancer
#3
Xing-Chun Peng, Zhi Zeng, Yu-Ning Huang, Yun-Chao Deng, Guo-Hui Fu
Transmembrane-4-L-six-family member-1 (TM4SF1), a tumor-associated antigen, is overexpressed in most epithelial cell carcinomas and a potential target for antibody-mediated therapy. However, the role of TM4SF1 in gastric cancer has not been elucidated. The aim of this study was to investigate the clinical significance of TM4SF1 expression in gastric carcinoma (GC) tissues using 152 GC tissue samples and matched adjacent nontumor tissue samples analyzed by immunohistochemistry, and 13 fresh GC tissue samples analyzed by Western blotting...
April 17, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29662627/analysis-of-the-pi3k-akt-mtor-pathway-in-penile-cancer-evaluation-of-a-therapeutically-targetable-pathway
#4
Anthony Adimonye, Elzbieta Stankiewicz, Sakunthala Kudahetti, Giorgia Trevisan, Brendan Tinwell, Cathy Corbishley, Yong-Jie Lu, Nick Watkin, Daniel Berney
Objectives: To determine whether phosphatidylinositol-4,5-bisphosphate 3- kinase, catalytic subunit alpha (PIK3CA) copy number gain is common and could prove a useful marker for the activation status of the PI3K-AKT-mTOR pathway in penile squamous cell carcinoma (PSCC). Methods: Fresh frozen tissue and archival blocks were collected from 24 PSCC patients with 15 matched normal penile epithelium (NPE) tissue from St George's Hospital. PIK3CA mutational and copy number status (CNS) was assessed via Sanger sequencing and fluorescence in-situ hybridisation, respectively...
March 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29660627/positive-effect-of-resveratrol-against-preantral-follicles-degeneration-after-ovarian-tissue-vitrification
#5
Carina Diniz Rocha, Mayara Mafra Soares, Deize de Cássia Antonino, Jairo Melo Júnior, Renata Ferreira Freitas Mohallem, Ana Paula Ribeiro Rodrigues, José Ricardo Figueiredo, Marcelo Emílio Beletti, José Octavio Jacomini, Benner Geraldo Alves, Kele Amaral Alves
This study aimed to evaluate whether the addition of resveratrol to vitrification/thawing medium improves the cryotolerance of preantral follicles enclosed in bovine ovarian fragments. Ovarian fragments were obtained from bovine fetuses and distributed to the following groups: fresh ovarian fragments (control), vitrified (VIT), and vitrified with resveratrol (VIT + RESV). Overall, the mean percentage of normal follicles was greater (P < 0.05) in the VIT + RESV compared to the VIT group. Moreover, the probability of finding normal follicles was 2...
April 6, 2018: Theriogenology
https://www.readbyqxmd.com/read/29660167/muse-microscopy-with-uv-surface-excitation-a-novel-approach-to-real-time-inexpensive-slide-free-dermatopathology
#6
Amir Qorbani, Farzad Fereidouni, Richard Levenson, Sana Yasmine Lahoubi, Zachary T Harmany, Austin Todd, Maxwell A Fung
Traditional histology relies on processing and physically sectioning either frozen or formalin-fixed, paraffin-embedded (FFPE) tissue into thin slices (typically 4-6 microns) prior to staining and viewing on a standard wide-field microscope. MUSE represents a novel alternative microscopy method that works with ultraviolet (UV) excitation using oblique cis-illumination which can generate high-quality images from the cut surface of fresh or fixed tissue after brief staining, with no requirement for fixation, embedding and histological sectioning of tissue specimens...
April 16, 2018: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/29660116/a-novel-histochemistry-assay-to-assess-and-quantify-focal-cytochrome-c-oxidase-deficiency
#7
Marie-Lune Simard, Arnaud Mourier, Laura C Greaves, Robert W Taylor, James B Stewart
Defects in the respiratory chain, interfering with energy production in the cell, are major underlying causes of mitochondrial diseases. In spite of this, the surprising variety of clinical symptoms, disparity between ages of onset as well as the involvement of mitochondrial impairment in ageing and age-related diseases, continues to challenge our understanding of the pathogenic processes. This complexity can be in part attributed to the unique metabolic needs of organs or of various cell types. In this view, it remains essential to investigate mitochondrial dysfunction at the cellular level...
April 16, 2018: Journal of Pathology
https://www.readbyqxmd.com/read/29658229/high-speed-raman-encoded-molecular-imaging-of-freshly-excised-tissue-surfaces-with-topically-applied-serrs-nanoparticles
#8
Yu Winston Wang, Qian Yang, Soyoung Kang, Matthew A Wall, Jonathan T C Liu
Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) are increasingly being engineered for a variety of disease-detection and treatment applications. For example, we have previously developed a fiber-optic Raman-encoded molecular imaging (REMI) system for spectral imaging of biomarker-targeted SERS NPs topically applied on tissue surfaces to identify residual tumors at surgical margins. Although accurate tumor detection was achieved, the commercial SERS NPs used in our previous studies lacked the signal strength to enable high-speed imaging with high pixel counts (large fields of view and/or high spatial resolution), which limits their use for certain time-constrained clinical applications...
April 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29657239/application-of-a-fresh-decellularized-pulmonary-allograft-for-pulmonary-valve-replacement-in-japan
#9
Hideto Ozawa, Takayoshi Ueno, Masaki Taira, Koichi Toda, Toru Kuratani, Yoshiki Sawa
BACKGROUND: Tissue engineering has advanced the technique of decellularization of the heart valve. The valve is reseeded with the patient's own cells after implantation with suppression of immunologic reactions. The same advantage has been reported for fresh decellularized heart valves, and more than 10 years of excellent outcomes have been achieved. We began performing such heart valve implantations in 2013 as part of a clinical study at Osaka University. We report our evaluation of the safety and efficacy of heart valve implantation...
April 14, 2018: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/29655900/the-anterolateral-ligament-is-a-distinct-ligamentous-structure-a-histological-explanation
#10
Thomas Neri, Pierre Dalcol, Fabien Palpacuer, Florian Bergandi, Jean Michel Prades, Frederic Farizon, Remi Philippot, Michel Peoc'h
BACKGROUND: The aim was to determine whether the anterolateral ligament (ALL) had a histological structure that defined it as a real ligament. METHODS: Histological examination of 30 ALL samples taken from fresh-frozen knees were performed. The ALL femoral insertion and its relationship with the lateral collateral ligament (LCL) were studied and the tibial insertion and its relationship with articular cartilage of the tibial joint surface were analyzed. For the ligamentous part, its histological structure and its differences with the articular capsule were studied...
April 11, 2018: Knee
https://www.readbyqxmd.com/read/29655850/biomonitoring-role-of-some-cellular-markers-during-heat-stress-induced-changes-in-highly-representative-fresh-water-mollusc-bellamya-bengalensis-implication-in-climate-change-and-biological-adaptation
#11
Sangita Maiti Dutta, Soumyajit Banerjee Mustafi, Sanghamitra Raha, Susanta Kumar Chakraborty
Owing to increasing concern of global climate-change, temperature rise is of great interest which can be primarily evaluated from the seasonal variations in some organisms. Aquatic environment can be extremely stressful to its inhabitants because most of them are poikilothermous. In the present study, attempt was made to evaluate the biological effects of oxidative-stress and adaptive/antioxidant capacities during temperature variations (36-40 °C for 24hrs to 72hrs) in Bellamya bengalensis both in environmental and laboratory conditions by testing some biomarkers like HSP70, catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and glutathione reductase (GR)...
April 12, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29652763/-forearm-based-turnover-muscle-flaps-for-elbow-soft-tissue-reconstruction-a-comparison-of-regional-coverage-based-on-distal-flap-perfusion
#12
Yash J Avashia, Ronnie L Shammas, Luke P Poveromo, Travis J Dekker, Jacob W Brubacher, Marc J Richard, David S Ruch, Suhail K Mithani
BACKGROUND: Elbow wounds pose a reconstructive challenge. Prior studies have described the vascular anatomy of both the brachioradialis (BR) and flexor carpi ulnaris (FCU) muscle flaps. The goal of this study was to describe the distal flap perfusion of the flexor carpi radialis (FCR), with a direct comparison of the BR, FCU, and FCR muscle flaps for coverage around the elbow. METHODS: Six fresh-frozen upper extremity specimens were dissected for BR, FCR, and FCU flaps...
March 28, 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29652604/an-engineered-human-adipose-collagen-model-for-in-vitro-breast-cancer-cell-migration-studies
#13
Robert David Hume, Lorraine Berry, Stefanie Reichelt, Michael D'Angelo, Jenny Gomm, Ruth Elizabeth Cameron, Christine Watson
Adipocytes are one of the major stromal cell components of the human breast. These cells play a key role in the development of the gland and are implicated in breast tumorigenesis. Frequently, directional stromal collagen I fibres are found surrounding aggressive breast tumours. These fibres enhance breast cancer cell migration and are associated with poor patient prognosis. We sought to recapitulate these stromal components in vitro to provide a 3D model comprising human adipose tissue and anisotropic collagen fibres...
March 8, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29650375/vascularized-distal-radius-bone-graft-for-treatment-of-ulnar-nonunion
#14
REVIEW
Ezequiel Ernesto Zaidenberg, Ezequiel Martinez, Carlos Rodolfo Zaidenberg
Various methods have been reported to treat forearm nonunions with good results. However, in the presence of infection, inadequate vascularity of surrounding tissues, or failed prior grafts, vascularized bone grafts are a valid alternative. We describe the surgical technique to obtain distal radius vascularized bone graft pedicled on the radial artery (RA) and its clinical application in 1 case of an ulnar nonunion. We studied the surgical technique in 12 freshly injected cadavers. In the distal forearm, the RA provides several periosteal branches to supply the distal radius metaphysis...
April 9, 2018: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29649332/development-of-novel-est-ssr-markers-for-ploidy-identification-based-on-de-novo-transcriptome-assembly-for-misgurnus-anguillicaudatus
#15
Bing Feng, Soojin V Yi, Manman Zhang, Xiaoyun Zhou
The co-existence of several ploidy types in natural populations makes the cyprinid loach Misgurnus anguillicaudatus an exciting model system to study the genetic and phenotypic consequences of ploidy variations. A first step in such effort is to identify the specific ploidy of an individual. Currently popular methods of karyotyping via cytological preparation or flow cytometry require a large amount of tissue (such as blood) samples, which can be damaging or fatal to the fishes. Here, we developed novel microsatellite markers (SSR markers) from M...
2018: PloS One
https://www.readbyqxmd.com/read/29648937/endothelial-wound-repair-of-the-organ-cultured-porcine-corneas
#16
Ingrida Smeringaiova, Stanislava Reinstein Merjava, Zbynek Stranak, Pavel Studeny, Jan Bednar, Katerina Jirsova
PURPOSE: To assess whether injured porcine endothelium of small and large corneoscleral disc differ in its reparative/regenerative capacity under various conditions of organ culture storage. MATERIAL AND METHODS: 166 paired porcine corneas were trephined to obtain tissues with diameter 12.0 mm and 17.5 mm (with area neighboring endothelial periphery). In tested discs, central endothelium was mechanically wounded. Density of live endothelial cells (LECD), percentage of dead cells (%DC), coefficient of variation and cell hexagonality were assessed in central and paracentral endothelium following 5- or 9-day incubation in medium with 2% or 10% fetal bovine serum...
April 12, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29643810/localized-delivery-of-cl-amidine-from-electrospun-polydioxanone-templates-to-regulate-acute-neutrophil-netosis-a-preliminary-evaluation-of-the-pad4-inhibitor-for-tissue-engineering
#17
Allison E Fetz, Indira Neeli, Karyl K Buddington, Robert W Read, Matthew P Smeltzer, Marko Z Radic, Gary L Bowlin
Upon interaction, neutrophils can potentially release neutrophil extracellular traps (NETs) on the surface of an implanted electrospun template, which may be a significant preconditioning event for implantable biomaterials of yet unknown consequences. In this study, we investigated the potential of polydioxanone templates as a delivery vehicle for Cl-amidine, an inhibitor of peptidyl arginase deiminase 4 (PAD4), and if drug elution could attenuate PAD4-mediated NETosis in the vicinity of implanted templates...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29643528/-comparison-of-the-properties-of-cd146-positive-and-cd146-negative-subpopulations-of-stem-cells-from-human-exfoliated-deciduous-teeth
#18
X T Wang, N Q Rao, T Jz Fang, Y M Zhao, L H Ge
OBJECTIVE: Stem cells from human exfoliated teeth (SHED) were sorted by magnetically activated cell sorting (MACS) technique to obtain the CD146 positive and negative cell subpopulation. Then the biological characteristics of these subpopulations were compared to explore their specific application potential in tissue engineering. METHODS: In this study, freshly extracted deciduous teeth without any caries or dental pulp disease were obtained. SHED was isolated using enzyme digestion method and then sorted by MACS, CD146 positive cells and CD146 negative cells were obtained after cell sorting...
April 18, 2018: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29630117/urg4-silencing-suppresses-cell-proliferation-in-nasopharyngeal-carcinoma-through-induction-of-apoptosis
#19
X-Y Luo, B Yan, C-F Cai, A-M Chen, M Zhou
OBJECTIVE: To investigate the expression and function of up-regulator of gene-4 (URG4) in nasopharyngeal carcinoma (NPC). PATIENTS AND METHODS: Fresh NPC tumor tissue samples with paired adjacent normal nasopharyngeal tissues samples of 9 NPC patients were collected from NPC curative resection surgery. NPC cell lines (CNE1, CNE2 and HONE1) were cultured. Lentivirus-mediated URG4-specific short hairpin RNA (shRNA) stable transfection was done. The effect of URG4 on CNE4 and HONE1 cells viability was determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT)...
March 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29629777/using-a-novel-supramolecular-gel-cryopreservation-system-in-microchannel-to-minimize-the-cell-injury
#20
Dong Xu Lan, Xi Chen, Pengcheng Li, Wei Zou, Lili Wu, Wanyu Chen
The storage of living cells is the major challenge for cell research and cell treatment. Here, we introduced a novel supramolecular gel cryopreservation system which was prepared in microchannel and the supramolecular gel (BDTC) was self-assembled by gelator Boc-O-dodecyl-L-tyrosine (BDT). This cryopreservation system could obviously minimize the cell injury because BDTC supramolecular gel had a more compact three-dimensional network structure when BDT gelator self-assembled in the confined space of microchannel...
April 9, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
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