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https://www.readbyqxmd.com/read/28429353/roles-of-the-nrf2-ho-1-pathway-in-the-anti-oxidative-stress-response-to-ischemia-reperfusion-brain-injury-in-rats
#1
L-J Jiang, S-M Zhang, C-W Li, J-Y Tang, F-Y Che, Y-C Lu
OBJECTIVE: The aim of this study was to investigate the roles of the Nrf2/HO-1 pathway in the responses to the oxidative stress created by ischemia-reperfusion brain injury in rats. MATERIALS AND METHODS: 54 healthy, adult, male SD rats were included in the study. Eighteen (18) rats were placed in the sham group. The ischemia-reperfusion model was created in the other 36 rats, among which 18 received injections of Nrf2 agonist before the surgery. The suture method was used to create artery occlusions in the right brain of the rats; and reperfusion was done after 90-minute ischemia (MCAO); while no suture was inserted in the sham group...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28427242/epidermal-growth-factor-receptor-variant-iii-in-head-and-neck-squamous-cell-carcinoma-is-not-relevant-for-targeted-therapy-and-irradiation
#2
Dominik Thomas Koch, Anja Pickhard, Lena Gebel, Anna Maria S Buchberger, Murat Bas, Carolin Mogler, Rudolf Reiter, Guido Piontek, Markus Wirth
BACKGROUND: The epidermal growth factor receptor (EGFR) is an important regulator of cell growth and survival, and is highly variable in tumor cells. The most prevalent variation of the EGFR extracellular domain is the EGFR variant III (EGFRvIII). Some studies imply that EGFRvIII may be responsible for the poor response to the monoclonal EGFR-antibody Cetuximab, used therapeutically in head and neck squamous cell carcinoma (HNSCC). Due to inconsistent data in the literature regarding EGFRvIII prevalence and clinical relevance in HNSCC, especially its predictive value, we examined EGFRvIII-transfected cell lines and patient tissue samples...
March 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28426133/quantitative-phase-microscopy-spatial-signatures-of-cancer-cells
#3
Darina Roitshtain, Lauren Wolbromsky, Evgeny Bal, Hayit Greenspan, Lisa L Satterwhite, Natan T Shaked
We present cytometric classification of live healthy and cancerous cells by using the spatial morphological and textural information found in the label-free quantitative phase images of the cells. We compare both healthy cells to primary tumor cells and primary tumor cells to metastatic cancer cells, where tumor biopsies and normal tissues were isolated from the same individuals. To mimic analysis of liquid biopsies by flow cytometry, the cells were imaged while unattached to the substrate. We used low-coherence off-axis interferometric phase microscopy setup, which allows a single-exposure acquisition mode, and thus is suitable for quantitative imaging of dynamic cells during flow...
April 20, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28425429/evidence-of-horizontal-transmission-of-the-cancer-associated-steamer-retrotransposon-among-ecological-cohort-bivalve-species
#4
Ashley N Paynter, Michael J Metzger, Jocelyn A Sessa, Mark E Siddall
Bivalve specimens from legacy frozen tissue collections, and others freshly obtained, were surveyed for the presence of the Steamer long terminal repeat (LTR)-retrotransposon associated with disseminated hemic neoplasia of the soft-shelled clam Mya areneria. Of 22 species investigated using primers for the pol region, only Atlantic M. arenaria, Atlantic and North Sea razor clams Ensis directus, and Baltic clams Macoma balthica from the North Sea were found to possess copies of Steamer in their genomes. Notably, close relatives like Mya truncata and Siliqua patula did not exhibit evidence of Steamer...
April 20, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28424665/ca-similichlamydia-in-epitheliocystis-co-infection-of-gilthead-seabream-gills-unique-morphological-features-of-a-deep-branching-chlamydial-family
#5
Helena M B Seth-Smith, Pantelis Katharios, Nancy Dourala, José M Mateos, Alexander G J Fehr, Lisbeth Nufer, Maja Ruetten, Maricruz Guevara Soto, Lloyd Vaughan
The Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) bacterial superphylum constitutes a broad range of organisms with an intriguing array of ultrastructural morphologies, including intracellular membranes and compartments and their corresponding complex genomes encoding these forms. The phylum Chlamydiae are all obligate intracellular bacteria and, although much is already known of their genomes from various families and how these regulate the various morphological forms, we know remarkably little about what is likely the deepest rooting clade of this phylum, which has only been found to contain pathogens of marine and fresh water vertebrates...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28423512/kinase-analysis-of-penile-squamous-cell-carcinoma-on-multiple-platforms-to-identify-potential-therapeutic-targets
#6
Eddy S Yang, Christopher D Willey, Amitkumar Mehta, Michael R Crowley, David K Crossman, Dongquan Chen, Joshua C Anderson, Gurudatta Naik, Deborah L Della Manna, Tiffiny S Cooper, Guru Sonpavde
Penile squamous cell carcinoma (PSCC) is an orphan malignancy with poorly understood biology and suboptimal systemic therapy. Given that kinases may be drivers and readily actionable, we performed comprehensive multiplatform analysis of kinases in PSCC tumor and normal tissue. Fresh frozen tumors were collected from 11 patients with PSCC. After macrodissection to demarcate tumor from normal tissue, the samples underwent multiplatform analysis of kinases. Next Generation Sequencing (NGS) of 517 kinase genes was performed using Agilent Kinome capture and run on the Illumina MiSeq at PE150bp...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421497/chapter-13-human-ovarian-tissue-vitrification
#7
Sherman Silber
Ovarian freezing and transplantation has garnered increasing interest as a potential way of preserving fertility in cancer patients as well as for women who just wish to delay childbearing. This chapter spells out our techniques of ovarian cortex vitrification and results for frozen compared to fresh ovarian cortex transplantation (in one single series from one center for the sake of consistency), as well as potentially provides insight into the mechanism behind ovarian follicle recruitment. This represents an effort to simplify and popularize an approach that has yielded favorable results (all cases recovered ovulation and 75% had successful spontaneous pregnancy) in one single, disciplined study...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28420812/isolation-of-mesenchymal-stromal-stem-cells-from-cryopreserved-umbilical-cord-blood-cells
#8
Sumie Fujii, Yasuo Miura, Masaki Iwasa, Satoshi Yoshioka, Aya Fujishiro, Noriko Sugino, Hitomi Kaneko, Yoko Nakagawa, Hideyo Hirai, Akifumi Takaori-Kondo, Tatsuo Ichinohe, Taira Maekawa
Umbilical cord blood (UCB) has advantages over other tissues because it can be obtained without an invasive procedure and complex processing. We explored the availability of cryopreserved UCB cells as a source of mesenchymal stromal/stem cells (MSCs). MSCs were successfully isolated from six of 30 UCB units (median volume, 34.0 mL; median nucleated cell number, 4.4×10(8)) that were processed and cryopreserved using CP-1/human serum albumin. This isolation rate was lower than that (57%) from non-cryopreserved UCB cells...
April 18, 2017: Journal of Clinical and Experimental Hematopathology: JCEH
https://www.readbyqxmd.com/read/28420435/comparison-of-the-mechanical-properties-of-different-skin-sites-for-auricular-and-nasal-reconstruction
#9
M F Griffin, B C Leung, Y Premakumar, M Szarko, P E Butler
BACKGROUND: Autologous and synthetic nasal and auricular frameworks require skin coverage. The surgeon's decides on the appropriate skin coverage for reconstruction based on colour matching, subcutaneous tissue thickness, expertise and experience. One of the major complications of placing subcutaneous implants is the risk of extrusion (migration through the skin) and infection. However, knowledge of lessening the differential between the soft tissue and the framework can have important implications for extrusion...
April 18, 2017: Journal of Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/28418278/the-effect-of-surgical-insertion-and-proinflammatory-cytokines-on-osteochondral-allograft-survival-and-metabolism
#10
Samantha L Gitelis, Ariel Bodker, Michel P Laurent, Spencer S Kirk, Giuseppe Filardo, Maximilian A Meyer, Arnavaz A Hakimiyan, Lev Rappoport, Markus A Wimmer, Brian J Cole, Susan Chubinskaya
Objective To investigate the responses of refrigerated osteochondral allograft cartilage (OCA) and fresh cartilage (FC), including cell survival and metabolism, to surgical impaction and proinflammatory cytokines. Design Osteochondral plugs (8 mm diameter) were harvested from prolonged-refrigerated (14-28 days) and fresh (≤24 hours postmortem) human femoral hemicondyles and subjected to a 0.2 N s pneumatic impaction impulse. Cartilage explants were removed from subchondral bone and randomized to 1 of 6 treatment groups: (1) Unimpacted control (UIC), (2) Impacted control (IC), (3) Impacted + interleukin (IL)-1β (0...
January 1, 2017: Cartilage
https://www.readbyqxmd.com/read/28414692/neuronal-fibers-and-neurotransmitter-receptor-expression-in-the-human-endolymphatic-sac
#11
Martin Nue Møller, Svend Kirkeby, Jonas Vikeså, Finn Cilius Nielsen, Per Cayé-Thomasen
INTRODUCTION: Recent studies suggest that the human endolymphatic sac (ES) may have multiple functions, including an ion-transport capacity comparable to the kidney, an immunological capacity and a possible natriuretic capacity. Further, there have been speculations of a yet undefined role in intracranial pressure homeostasis. The anatomical location towards the sigmoid sinus would suggest a possible endo- and/or paracrine signaling. However, neuronal connections may also apply, but it remains very scarcely explored in the human ES...
April 14, 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28412975/effect-of-cryopreservation-on-proliferation-and-differentiation-of-periodontal-ligament-stem-cell-sheets
#12
Mengying Li, Cheng Feng, Xiuge Gu, Qin He, Fulan Wei
BACKGROUND: Cryopreservation has been extensively applied to the long-term storage of a diverse range of biological materials. However, no comprehensive study is currently available on the cryopreservation of periodontal ligament stem cell (PDLSC) sheets which have been suggested as excellent transplant materials for periodontal tissue regeneration. The aim of this study is to investigate the effect of cryopreservation on the structural integrity and functional viability of PDLSC sheets...
April 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28412973/genome-wide-dna-methylation-measurements-in-prostate-tissues-uncovers-novel-prostate-cancer-diagnostic-biomarkers-and-transcription-factor-binding-patterns
#13
Marie K Kirby, Ryne C Ramaker, Brian S Roberts, Brittany N Lasseigne, David S Gunther, Todd C Burwell, Nicholas S Davis, Zulfiqar G Gulzar, Devin M Absher, Sara J Cooper, James D Brooks, Richard M Myers
BACKGROUND: Current diagnostic tools for prostate cancer lack specificity and sensitivity for detecting very early lesions. DNA methylation is a stable genomic modification that is detectable in peripheral patient fluids such as urine and blood plasma that could serve as a non-invasive diagnostic biomarker for prostate cancer. METHODS: We measured genome-wide DNA methylation patterns in 73 clinically annotated fresh-frozen prostate cancers and 63 benign-adjacent prostate tissues using the Illumina Infinium HumanMethylation450 BeadChip array...
April 17, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28412565/the-use-of-a-novel-perfusion-based-cadaveric-simulation-model-with-cerebrospinal-fluid-reconstitution-comparing-dural-repair-techniques-a-pilot-study
#14
Joshua Bakhsheshian, Ben A Strickland, Neil N Patel, Andre M Jakoi, Michael Minneti, Gabriel Zada, Frank L Acosta, Patrick C Hsieh, Jeffrey C Wang, John C Liu, Martin H Pham
BACKGROUND CONTEXT: Watertight dural repair is crucial for both incidental durotomy and closure after intradural surgery. PURPOSE: To describe a perfusion-based cadaveric simulation model with cerebrospinal-fluid (CSF) reconstitution and to compare spine dural repair techniques. STUDY DESIGN/SETTING: Fresh Tissue Dissection Laboratory. SAMPLE SIZE: 8 fresh human cadavers. OUTCOME MEASURES: A watertight closure was achieved when pressurized saline up to 40 mm Hg did not cause further CSF leakage beyond the suture lines...
April 12, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28412187/restoring-volar-tilt-in-ao-type-c2-fractures-of-the-distal-radius-with-unilateral-external-fixation
#15
Kenneth F Taylor, David Gendelberg, Michael B Lustik, Matthew L Drake
PURPOSE: To determine whether unilateral external fixation using a staged multiplanar reduction technique restores anatomic volar tilt in a distal radius fracture model. METHODS: We used radiographic images to obtain baseline measurements in 20 fresh-frozen cadaveric wrists. Through a standard dorsal approach to the radius, we performed osteotomies to simulate displaced AO/ASIF type C2 fractures. After placement of a unilateral external fixator, a stepwise technique of applying longitudinal traction followed by a volar translational maneuver was performed...
April 12, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/28410240/ulk1-over-expression-in-human-gastric-cancer-is-correlated-with-patients-t-classification-and-cancer-relapse
#16
Min-Bin Chen, Xiao-Zhi Ji, Yuan-Yuan Liu, Ping Zeng, Xin-Yu Xu, Rong Ma, Zheng-Dong Guo, Jian-Wei Lu, Ji-Feng Feng
Ulk1 is a key autophagy protein. Here, we tested expression and potential function of Ulk1 in human gastric cancer. Ulk1 mRNA and protein were significantly elevated in multiple fresh human gastric cancer tissues. Its level was relatively low in surrounding normal epithelial tissues. Ulk1 over-expression was also observed in several gastric cancer cell lines (AGS, HGC-27, and SNU601). Remarkably, Ulk1 knockdown by targeted-shRNA inhibited AGS gastric cancer cell survival and proliferation. On the other hand, exogenous Ulk1 over-expression could further promote AGS cell survival and proliferation...
March 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/28408749/high-prevalence-of-tp53-mutations-is-associated-with-poor-survival-and-an-emt-signature-in-gliosarcoma-patients
#17
Sung-Yup Cho, Changho Park, Deukchae Na, Jee Yun Han, Jieun Lee, Ok-Kyoung Park, Chengsheng Zhang, Chang Ohk Sung, Hyo Eun Moon, Yona Kim, Jeong Hoon Kim, Jong Jae Kim, Shin Kwang Khang, Do-Hyun Nam, Jung Won Choi, Yeon-Lim Suh, Dong Gyu Kim, Sung Hye Park, Hyewon Youn, Kyuson Yun, Jong-Il Kim, Charles Lee, Sun Ha Paek, Hansoo Park
Gliosarcoma (GS) is a rare variant (2%) of glioblastoma (GBM) that poses clinical genomic challenges because of its poor prognosis and limited genomic information. To gain a comprehensive view of the genomic alterations in GS and to understand the molecular etiology of GS, we applied whole-exome sequencing analyses for 28 GS cases (6 blood-matched fresh-frozen tissues for the discovery set, 22 formalin-fixed paraffin-embedded tissues for the validation set) and copy-number variation microarrays for 5 blood-matched fresh-frozen tissues...
April 14, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28408349/early-effects-of-prolonged-cardiac-arrest-and-ischemic-postconditioning-during-cardiopulmonary-resuscitation-on-cardiac-and-brain-mitochondrial-function-in-pigs
#18
Timothy R Matsuura, Jason A Bartos, Adamantios Tsangaris, Kadambari Chandra Shekar, Matthew D Olson, Matthias L Riess, Martin Bienengraeber, Tom P Aufderheide, Robert W Neumar, Jennifer N Rees, Scott H McKnite, Anna E Dikalova, Sergey I Dikalov, Hunter F Douglas, Demetris Yannopoulos
Background Out-of-hospital cardiac arrest (CA) is a prevalent medical crisis resulting in severe injury to the heart and brain and an overall survival of less than 10 percent. Mitochondrial dysfunction is predicted to be a key determinant of poor outcomes following prolonged CA. However, the onset and severity of mitochondrial dysfunction during CA and cardiopulmonary resuscitation (CPR) is not fully understood. Ischemic postconditioning (IPC), controlled pauses during the initiation of CPR, has been shown to improve cardiac function and neurologically favorable outcomes after fifteen minutes of CA...
April 10, 2017: Resuscitation
https://www.readbyqxmd.com/read/28408210/the-organic-anion-transporting-peptide-oatp-2b1-is-localized-in-the-basolateral-membrane-of-the-human-jejunum-and-caco-2-monolayers
#19
Markus Keiser, Lars Kaltheuner, Charlotte Wildberg, Janett Müller, Markus Grube, Lars Ivo Partecke, Claus-Dieter Heidecke, Stefan Oswald
The organic anion transporting polypeptide (OATP) 2B1 which is ubiquitously expressed in the human body is assumed to play an important role in the cellular uptake of many drugs. While the expression and function of this SLC transporter is well characterized in the human liver, little is known about its localization and functional relevance in the intestine. Thus, it was the aim of this study to investigate its localization and function in the human jejunum and in the frequently used intestinal Caco-2 cell line...
April 10, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28406926/high-resolution-respirometry-of-fine-needle-muscle-biopsies-in-pre-manifest-huntington-s-disease-expansion-mutation-carriers-shows-normal-mitochondrial-respiratory-function
#20
Eva Buck, Martina Zügel, Uwe Schumann, Tamara Merz, Anja M Gumpp, Anke Witting, Jürgen M Steinacker, G Bernhard Landwehrmeyer, Patrick Weydt, Enrico Calzia, Katrin S Lindenberg
Alterations in mitochondrial respiration are an important hallmark of Huntington's disease (HD), one of the most common monogenetic causes of neurodegeneration. The ubiquitous expression of the disease causing mutant huntingtin gene raises the prospect that mitochondrial respiratory deficits can be detected in skeletal muscle. While this tissue is readily accessible in humans, transgenic animal models offer the opportunity to cross-validate findings and allow for comparisons across organs, including the brain...
2017: PloS One
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