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https://www.readbyqxmd.com/read/29470708/the-wound-response-in-fresh-cut-lettuce-involves-programmed-cell-death-events
#1
Elena T Iakimova, Ernst J Woltering
In this work, the involvement of programmed cell death (PCD) in the wound-induced postharvest browning disorder and senescence in butterhead lettuce (Lactuca sativa L.) fresh-cuts was studied. At the wounded (cut, bruised) sites, rapid browning, loss of chlorophyll and massive cell death, accompanied with accumulation of reactive oxygen species and increased electrolyte leakage occurred in a narrow strip of tissue adjacent the injury. The dead cell morphology (protoplast and nuclei shrinkage) together with the biochemical and physiological changes resembled necrotic PCD type...
February 22, 2018: Protoplasma
https://www.readbyqxmd.com/read/29470232/intraoperative-extracorporeal-irradiation-and-frozen-treatment-on-tumor-bearing-autografts-show-equivalent-outcomes-for-biologic-reconstruction
#2
Po-Kuei Wu, Cheng-Fong Chen, Chao-Ming Chen, Yu-Chi Cheng, Shang-Wen Tsai, Tain-Hsiung Chen, Wei-Ming Chen
BACKGROUND: Immediately recycling the resected bone segment in a biologic limb salvage reconstruction is an option after wide resection of bone. Intraoperative extracorporeal irradiation and freezing are the two major tumor-killing techniques applied on the fresh tumor-bearing autografts. However, graft-derived tumor recurrence and complications are concerns affecting graft survival. QUESTIONS/PURPOSES: We therefore asked: (1) Is there a difference in the proportion of patients achieving union by 18 months after surgery between the groups with extracorporeal-irradiated autografts and frozen-treated autografts? (2) Is there any difference in the frequency of graft-related complications for patients receiving either an extracorporeal-irradiated or a frozen-treated autograft? (3) Is there a difference between the techniques in terms of graft-derived recurrence? (4) Are there differences in failure-free grafts, and limb and overall survivorship between autografts treated by extracorporeal irradiation or by freezing? METHODS: During the study period we treated a total of 333 patients with high-grade osteosarcoma...
February 21, 2018: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/29467942/-in-vitro-in-vivo-and-ex-vivo-demonstration-of-the-antitumoral-role-of-hypocretin-1-orexin-a-and-almorexant-in-pancreatic-ductal-adenocarcinoma
#3
Stéphanie Dayot, Daniela Speisky, Anne Couvelard, Pierre Bourgoin, Valérie Gratio, Jérôme Cros, Vinciane Rebours, Alain Sauvanet, Pierre Bedossa, Valérie Paradis, Philippe Ruszniewski, Alain Couvineau, Thierry Voisin
Pancreatic ductal adenocarcinoma (PDAC) is still the poorest prognostic tumor of the digestive system. We investigated the antitumoral role of orexin-A and almorexant in PDAC. We analyzed the orexin receptor type 1 (OX1R) expression by immunohistochemistry in human normal pancreas, PDAC and its precursor dysplastic intraepithelial lesions. We used PDAC-derived cell lines and fresh tissue slices to study the apoptotic role of hypocretin-1/orexin-A and almorexant in vitro and ex vivo . We analyzed in vivo the hypocretin-1/orexin-A and almorexant effect on tumor growth in mice xenografted with PDAC cell lines expressing, or not, OX1R...
January 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29465782/dielectric-properties-for-non-invasive-detection-of-normal-benign-and-malignant-breast-tissues-using-microwave-theories
#4
Yiou Cheng, Minghuan Fu
BACKGROUND: Despite the high incidence of breast cancer worldwide, methods for early non-invasive diagnosis and sensitive and specific prognostic evaluation remain difficult. In this study, we investigated microwave parameters as a potential non-invasive approach to detect breast cancer. METHODS: Samples of freshly excised breast tissues (n = 509) from 98 patients were identified as normal, benign tumor, or malignant cancer via histology. Further samples were prepared and the microwave effective dielectric permittivity and effective conductivity were measured every 0...
February 21, 2018: Thoracic Cancer
https://www.readbyqxmd.com/read/29464165/reexploring-the-anatomy-of-the-distal-humerus-for-its-role-in-providing-vascularized-bone
#5
Logan W Carr, Sebastian M Brooke, T Shane Johnson, Brett F Michelotti
Background: The lateral arm flap is used for composite defects in need of vascularized soft tissue, skin, and bone. From its original description, the distal humeral metaphysis can be included with the flap, supplied by the periosteal extensions of the posterior branch of the radial collateral artery. We sought to reexplore the anatomy of the lateral arm to determine its utility as a donor site for vascularized bone. Methods: Twelve fresh, silicone-injected cadaver dissections were performed...
January 2018: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/29462875/extensive-degradation-and-low-bioavailability-of-orally-consumed-corn-mirnas-in-mice
#6
Haiqiu Huang, Cindy D Davis, Thomas T Y Wang
The current study seeks to resolve the discrepancy in the literature regarding the cross-kingdom transfer of plant microRNAs (miRNAs) into mammals using an improved miRNA processing and detection method. Two studies utilizing C57BL/6 mice were performed. In the first study, mice were fed an AIN-93M diet and gavaged with water, random deoxynucleotide triphosphates (dNTP) or isolated corn miRNAs for two weeks ( n = 10 per group). In the second study, mice were fed an AIN-93M diet, or the diet supplemented with 3% fresh or autoclaved corn powder for two weeks ( n = 10 per group)...
February 15, 2018: Nutrients
https://www.readbyqxmd.com/read/29462199/high-quality-diet-improves-lipid-metabolic-profile-and-breeding-performance-in-the-blue-footed-booby-a-long-lived-seabird
#7
Erick González-Medina, José Alfredo Castillo-Guerrero, Sharon Zinah Herzka, Guillermo Fernández
Understanding the role of diet in the physiological condition of adults during reproduction and hence its effect on reproductive performance is fundamental to understand reproductive strategies in long-lived animals. In birds, little is known about the influence of the quality of food consumed at the beginning of the reproductive period and its short-term effects on reproductive performance. To assess the role of diet in the physiological condition of female blue-footed booby, Sula nebouxii (BFBO), during reproduction we evaluated whether individual differences in diet (assessed by using δ13C and δ15N values of whole blood from female birds and muscle tissue of the principal prey species) prior to egg laying and during incubation influenced their lipid metabolic profile (measured as triglyceride levels and C:N ratio) and their reproductive performance (defined by laying date, clutch size and hatching success)...
2018: PloS One
https://www.readbyqxmd.com/read/29461446/evaluating-the-utility-of-the-lateral-elbow-radiograph-in-central-articular-olecranon-reduction-an-anatomic-and-radiographic-study
#8
Jeremy Kubik, Prism Schneider, Richard Buckley, Robert Korley, Paul Duffy, Ryan Martin
OBJECTIVES: The surgical reduction of intra-articular olecranon fractures is judged primarily on the lateral elbow radiograph, as orthogonal imaging of the articular surface is not obtainable. We sought to determine surgeon accuracy in identifying intra-articular olecranon malreductions on the lateral elbow radiograph. METHODS: Six human fresh-frozen cadaveric elbow specimens were sagittally sectioned in 5-mm increments after olecranon dissection, preservation of soft tissue envelope, and rigid fixation of the elbow in an external fixator...
March 2018: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29461398/what-is-important-besides-getting-the-bone-to-heal-impact-on-tissue-injury-other-than-the-fracture
#9
Michael J Gardner, Thomas A Higgins, William H Harvin, James P Stannard, Mark A Lee, Brett D Crist
Fracture surgeons do a great job of managing bone issues, but they may overlook the associated soft tissue injuries that play a significant role in the final outcome after musculoskeletal injury. The soft tissue reconstruction ladder can help guide reconstructive procedures based on the least complex procedure that allows the best chance of fracture healing. Muscle injury, volume loss, and deconditioning occur with traumatic injury and during the recovery phase. Neuromuscular stimulation, nutrition, and strength training are potential ways to aid in recovery...
March 2018: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29460420/goat-milk-non-invasive-source-for-mammary-epithelial-cell-isolation-and-in-vitro-culture
#10
N Saipin, J Noophun, P Chumyim, R Rungsiwiwut
Mammary epithelial cells (MECs) used to study mammary gland function in vitro are typically isolated from mammary tissue. Breast milk offers an alternative source of MECs to replace mammary gland tissue. This study aimed to isolate and culture MECs from goat milk. Samples of fresh goat milk were collected from early and late-lactating Saanen goats. Samples were subjected to isolation, culture and characterization for putative MECs. The cells adhered to the culture dishes 1 day after isolation and displayed a monolayer pattern, epithelial-like cobblestone morphology, and formed alveoli-like structures that are typical of MECs...
February 19, 2018: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/29456687/girdin-protein-a-potential-metastasis-predictor-associated-with-prognosis-in-lung-cancer
#11
Zhaoyang Yang, Fang Yang, Yingli Zhang, Xin Wang, Jiong Shi, Hongjiao Wei, Fengwei Sun, Yan Yu
The present study explored the relationship between Girdin protein expression and the survival rate of patients with lung carcinoma. A total of 334 lung cancer specimens, 20 benign lung disease tissue sections and 24 fresh tissues from patients with lung carcinoma were analyzed by immunohistochemistry and western blotting. Girdin protein was expressed in 130/334 (38.93%) of the cases examined. Girdin protein expression was correlated with tumor/node/metastasis stage (P<0.001), lymph node metastasis (P=0...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29456341/using-of-fresh-cadaveric-cow-brain-in-the-microsurgical-training-model-for-sulcal-cisternal-and-fissural-dissection
#12
Ahmet Gökyar, Cengiz Cokluk
Objective: The aim of this experimental study was to evaluate the feasibility of using fresh cadaveric cow brain as in a training model for microsurgical dissection of sulcus, cisterns, and fissure. Methods: Experimental microneurosurgical activities in this study were performed under the operating microscope. Bilateral sylvian cisterns, interhemispheric fissure, and hemispheric sulcus of the fresh cadaveric cow brain were used as an interested area for this experimental study...
January 2018: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/29454891/a-new-procedure-for-processing-extracted-teeth-for-immediate-grafting-in-post-extraction-sockets-an-experimental-study-in-american-fox-hound-dogs
#13
José Luis Calvo Guirado, Pilar Cegarra Del Pino, Lari Sapoznikov, Rafael Arcesio Delgado Ruíz, Manuel Fernández Dominguez, Sérgio Alexandre Gehrke
OBJECTIVES: To investigate freshly extracted dental particulate used to graft post-extraction sockets in dogs, comparing new bone formation at experimental and control sites. MATERIAL AND METHODS: Bilateral premolars P2, P3, P4 and first mandibular molars were extracted atraumatically from six American Fox Hound dogs. The teeth were ground immediately using a 'Smart Dentin Grinder.' The dentin particulate was sieved to ensure a grain size of 300-1200μm and immersed in an alcohol cleanser to dissolve organic debris and bacteria, followed by washing in sterile saline buffer solution...
February 15, 2018: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29454543/characterization-of-the-passive-and-active-material-parameters-of-the-pubovisceralis-muscle-using-an-inverse-numerical-method
#14
M E T Silva, M P L Parente, S Brandão, T Mascarenhas, R M Natal Jorge
The mechanical characteristics of the female pelvic floor are relevant to understand pelvic floor dysfunctions (PFD), and how they are related with changes in their biomechanical behavior. Urinary incontinence (UI) and pelvic organ prolapse (POP) are the most common pathologies, which can be associated with changes in the mechanical properties of the supportive structures in the female pelvic cavity. PFD have been studied through different methods, from experimental tensile tests using tissues from fresh female cadavers or tissues collected at the time of a transvaginal hysterectomy procedure, or by applying imaging techniques...
February 8, 2018: Journal of Biomechanics
https://www.readbyqxmd.com/read/29453700/reliability-of-cartilage-digestion-and-fda-eb-fluorescence-staining-for-the-detection-of-chondrocyte-viability-in-osteochondral-grafts
#15
Jianhong Qi, Famin Cao, Yunning Han, Di Xie, Hongqiang Song, Bin Chen, Lu Zhou
The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular cartilage tissue was preserved. Each cartilage graft was cut into two 70-µm thick pieces and randomly allocated to Group A or Group B. The cell viability of Group A was detected using FDA-EB fluorescence staining of the digested cartilage, and the viability of Group B was detected with FDA-EB fluorescence staining of cartilage sections...
February 16, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29448960/double-negative-cd27-igd-b-cells-are-expanded-in-nsclc-and-inversely-correlate-with-affinity-matured-b-cell-populations
#16
Sara M Centuori, Cecil J Gomes, Samuel S Kim, Charles W Putnam, Brandon T Larsen, Linda L Garland, David W Mount, Jesse D Martinez
BACKGROUND: The presence of B cells in early stage non-small cell lung cancer (NSCLC) is associated with longer survival, however, the role these cells play in the generation and maintenance of anti-tumor immunity is unclear. B cells differentiate into a variety of subsets with differing characteristics and functions. To date, there is limited information on the specific B cell subsets found within NSCLC. To better understand the composition of the B cell populations found in NSCLC we have begun characterizing B cells in lung tumors and have detected a population of B cells that are CD79A + CD27 - IgD - ...
February 15, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29446263/pulsed-terahertz-imaging-of-breast-cancer-in-freshly-excised-murine-tumors
#17
Tyler Bowman, Tanny Chavez, Kamrul Khan, Jingxian Wu, Avishek Chakraborty, Narasimhan Rajaram, Keith Bailey, Magda El-Shenawee
This paper investigates terahertz (THz) imaging and classification of freshly excised murine xenograft breast cancer tumors. These tumors are grown via injection of E0771 breast adenocarcinoma cells into the flank of mice maintained on high-fat diet. Within 1 h of excision, the tumor and adjacent tissues are imaged using a pulsed THz system in the reflection mode. The THz images are classified using a statistical Bayesian mixture model with unsupervised and supervised approaches. Correlation with digitized pathology images is conducted using classification images assigned by a modal class decision rule...
February 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29445428/the-danger-zone-in-the-anterior-neck-anatomical-landmarks-to-avoid-injury-to-anterior-jugular-vein-during-face-lift-and-neck-lift
#18
Andrew M Swiergosz, J Stephen Gunn, Steven A Schulz, Joshua T Henderson, Joshua H Choo, Arian Mowlavi, Bradon J Wilhelmi
Background : An estimated 125,711 face-lifts and 54,281 neck-lifts were performed in 2015. Regardless of the technique employed, facial and neck flap elevation carries with it anatomical risk of which any surgeon performing these procedures should be aware of. Statistics related to anterior jugular vein injury during these procedures have not been published. Objective : To define a "danger zone" that will contain both of the anterior jugular veins on the basis of anatomical landmarks to aid surgeons with planning their surgical approach during rhytidectomy in the anterior neck region...
2018: Eplasty
https://www.readbyqxmd.com/read/29445296/the-ultrasound-guided-retrolaminar-block-volume-dependent-injectate-distribution
#19
Marija Damjanovska, Tatjana Stopar Pintaric, Erika Cvetko, Kamen Vlassakov
Purpose: The ultrasound-guided retrolaminar block is one of the newer and simpler alternatives to the traditional, often technically challenging, paravertebral (PV) block. Its feasibility, safety, and efficacy have already been clinically demonstrated in patients with multiple rib fractures using higher volumes of local anesthetic, when compared with the traditional approach. The primary aim of this observational anatomical study was to assess the spread of local anesthetic from the retrolaminar injection point to the PV space and its volume dependence...
2018: Journal of Pain Research
https://www.readbyqxmd.com/read/29444830/characterization-of-a-vesivirus-associated-with-an-outbreak-of-acute-hemorrhagic-gastroenteritis-in-domestic-dogs
#20
Randall W Renshaw, Jennifer Griffing, Jaime Weisman, Lisa M Crofton, Melissa A Laverack, Robert P Poston, Gerald E Duhamel, Edward J Dubovi
Four out of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence homology to a vesivirus within the group colloquially known as the 2117 strains that were originally identified as contaminants in CHO cell bioreactors. In situ hybridization and reverse transcription-PCR assays of tissues from the four deceased dogs confirmed the presence of canine vesivirus (CaVV) nucleic acids that localized to endothelial cells of arterial and capillary blood vessels...
February 14, 2018: Journal of Clinical Microbiology
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