keyword
MENU ▼
Read by QxMD icon Read
search

High protein in urine

keyword
https://www.readbyqxmd.com/read/28213844/self-nanoemulsified-drug-delivery-system-of-hydrochlorothiazide-for-increasing-dissolution-rate-and-diuretic-activity
#1
Cassiana Mendes, Aline Buttchevitz, Jéssica Henriques Kruger, Thiago Caon, Patricia de Oliveira Benedet, Elenara Lemos-Senna, Marcos Antônio Segatto Silva
Hydrochlorothiazide (HCTZ) is a class IV drug according to the Biopharmaceutical Classification System. This study aimed the development of self-nanoemulsifying drug delivery system (SNEDDS) for HCTZ as an approach to overcome the biopharmaceutical limitations. Pre-formulation screening and ternary phase diagrams were carried out to select the oil phase, the surfactant, and the co-surfactant as the amount of each constituent. The optimized formulations, with reduced amount of surfactant, and composed of medium chain triglycerides, Cremophor EL and Transcutol P did not affect the pH or show drug incompatibilities...
February 17, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28213648/-state-of-the-art-in-fluid-and-volume-therapy-a%C3%A2-user-friendly-staged-concept
#2
M Rehm, N Hulde, T Kammerer, A S Meidert, K Hofmann-Kiefer
Adequate fluid therapy is highly important for the perioperative outcome of our patients. Both, hypovolemia and hypervolemia can lead to an increase in perioperative complications and can impair the outcome. Therefore, perioperative infusion therapy should be target-oriented. The main target is to maintain the patient's preoperative normovolemia by using a sophisticated, rational infusion strategy.Perioperative fluid losses should be discriminated from volume losses (surgical blood loss or interstitial volume losses containing protein)...
February 17, 2017: Der Anaesthesist
https://www.readbyqxmd.com/read/28211531/proteomic-analysis-of-urinary-extracellular-vesicles-from-high-gleason-score-prostate-cancer
#3
Kazutoshi Fujita, Hideaki Kume, Kyosuke Matsuzaki, Atsunari Kawashima, Takeshi Ujike, Akira Nagahara, Motohide Uemura, Yasushi Miyagawa, Takeshi Tomonaga, Norio Nonomura
Extracellular vesicles (EVs) are microvesicles secreted from various cell types. We aimed to discover a new biomarker for high Gleason score (GS) prostate cancer (PCa) in urinary EVs via quantitative proteomics. EVs were isolated from urine after massage from 18 men (negative biopsy [n = 6], GS 6 PCa [n = 6], or GS 8-9 PCa [n = 6]). EV proteins were labeled with iTRAQ and analyzed by LC-MS/MS. We identified 4710 proteins and quantified 3528 proteins in the urinary EVs. Eleven proteins increased in patients with PCa compared to those with negative biopsy (ratio >1...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28193366/development-of-c60-based-labeling-reagents-for-the-determination-of-low-molecular-weight-compounds-by-matrix-assisted-laser-desorption-ionization-mass-i-determination-of-amino-acids-in-microliter-biofluids
#4
Pin Wu, Hua-Ming Xiao, Jun Ding, Qian-Yun Deng, Fang Zheng, Yu-Qi Feng
Quantification of low molecular weight compounds (<800 Da) using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI MS) is challenging due to the matrix signal interference at low m/z region and poor reproducibility of MS responses. In this study, a C60 labeling-MALDI MS strategy was proposed for the fast, sensitive and reliable determination of amino acids (AAs) in biofluids. An N-hydroxysuccinimide functionalized C60 was synthesized as the labeling reagent and added as an 880 Da tag to AAs; a carboxyl acid containing C60 was employed as the internal standards to normalize MS variations...
April 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28193058/development-of-a-biocompatible-in-tube-solid-phase-microextraction-device-a-sensitive-approach-for-direct-analysis-of-single-drops-of-complex-matrixes
#5
Hamed Piri-Moghadam, Sofia Lendor, Janusz Pawliszyn
The aim of the current study is to develop a sensitive solid-phase microextraction (SPME) device for direct and rapid analysis of untreated complex matrixes (i.e., single drop of the samples, V ≤ 2 μL). A thin layer of a biocompatible nanostructured polypyrrole (PPy) was electrochemically deposited inside a medical grade spinal needle, minimizing the matrix effect. Microsampling was facilitated by loading the sample inside the in-tube SPME device (withdraw of sample via plunger), where extraction was performed under static conditions...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28190859/renal-artery-stenosis-following-nilotinib-administration-in-a-patient-with-chronic-myelogenous-leukemia
#6
Mayumi Hatsuse, Yuka Daikoku, Yuta Tamoto, Masahiro Uehara, Takashi Kitani, Keiichi Tamagaki, Shin-Ichi Fuchida, Akira Okano, Satoshi Murakami, Chihiro Shimazaki
A 63-year-old male was diagnosed as having chronic phase CML in 2001. He obtained a major molecular response with imatinib (IM). In 2012, amulodipin was started for hypertension. In January 2013, IM was switched to nilotinib (NIL) in a clinical trial, and in February 2015, NIL was discontinued because MR(4.5) had been maintained for two years. One month later, he was admitted to our hospital because of headache and high blood pressure (194/108 mmHg). His urine test showed protein 3+ and occult blood 2+. His eGFR rapidly deteriorated from 45...
2017: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
https://www.readbyqxmd.com/read/28188437/clinical-utility-of-spot-urine-protein-to-creatinine-ratio-modified-by-estimated-daily-creatinine-excretion-in-children
#7
Eun Mi Yang, Bo Ae Yoon, Soo Wan Kim, Chan Jong Kim
BACKGROUND: The spot urine protein-to-creatinine ratio (UPCR) is widely used to predict 24-h urine protein (24-h UP) excretion. In patients with low daily urine creatinine excretion (UCr), however, the UPCR may overestimate 24-h UP. The aim of this study was to predict 24-h UP using UPCR adjusted by estimated 24-h UCr in children. METHODS: This study included 442 children whose 24-h UP and spot UPCR were measured concomitantly. Estimated 24-h UCr was calculated using three previously existing equations...
February 10, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28179422/viruses-comprise-an-extensive-pool-of-mobile-genetic-elements-in-eukaryote-cell-cultures-and-human-clinical-samples
#8
Jakob Thannesberger, Hans-Joerg Hellinger, Ingeborg Klymiuk, Marie-Theres Kastner, Franz J J Rieder, Martina Schneider, Susanne Fister, Thomas Lion, Karin Kosulin, Johannes Laengle, Michael Bergmann, Thomas Rattei, Christoph Steininger
Viruses shape a diversity of ecosystems by modulating their microbial, eukaryotic, or plant host metabolism. The complexity of virus-host interaction networks is progressively fathomed by novel metagenomic approaches. By using a novel metagenomic method, we explored the virome in mammalian cell cultures and clinical samples to identify an extensive pool of mobile genetic elements in all of these ecosystems. Despite aseptic treatment, cell cultures harbored extensive and diverse phage populations with a high abundance of as yet unknown and uncharacterized viruses (viral dark matter)...
February 8, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28164667/albuminuria-is-suggested-as-a-potential-health-screening-biomarker-for-senior-citizens-and-general-population-with-hypertension-or-diabetes-in-china
#9
Dongmei Gu, Pengcheng Xu, Yuhua Yuan, Hongwei Fu
BACKGROUND: To evaluate the possibility of albuminuria as a screening biomarker for seniors or general population with hypertension and diabetes. METHODS: 478 health check-up individuals were enrolled. Albumin to creatinine ratio (ACR) was calculated by testing urinary albumin and creatinine of spot urine sample. Each urine sample was also analyzed by the routine urine test in parallel with ACR. Potential risk factors associated with the presence of albuminuria were analyzed using independent t-test or chi-square test...
November 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28164659/analysis-of-four-automated-urinalysis-systems-compared-to-reference-methods
#10
Kamila Bartosova, Zdenek Kubicek, Janka Franekova, Gustav Louzensky, Petra Lavrikova, Antonin Jabor
BACKGROUND: The aim of this study was to compare four automated urinalysis systems: the Iris iQ200 Sprint (Iris Diagnostics, U.S.A.) combined with the Arkray AUTION MAX AX 4030, Iris + AUTION, Arkray AU 4050 (Arkray Global Business, Inc., Japan), Dirui FUS 2000 (Dirui Industrial Co., P.R.C.), and Menarini sediMAX (Menarini, Italy). METHODS: Urine concentrations of protein and glucose (Iris, Dirui) were compared using reference quantitative analysis on an Abbott Architect c16000...
November 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28153286/functional-monomer-template-qds-sandwich-structure-for-mesoporous-structured-bovine-hemoglobin-imprinted-ratiometric-fluorescence-sensor
#11
Lu Hongzhi, Xu Shoufang
In this work, mesoporous structured molecularly imprinted ratiometric fluorescence sensor (M-R-MIPs) for highly sensitive and selective determination of bovine hemoglobin (BHb) was developed based on core-shell structure. First, red CdTe QDs were embedded into silica nanoparticles by reverse microemulsion method, then BHb imprinted silica shell were coated on the core silica. In order to improve the sensitivity of the as-prepared MIPs sensor, during the process of imprinted silica shell formation, functional monomer-template-QDs sandwich structure was first employed to improve the recognition sites accessibility, mesoporous silica was applied to reduce mass transfer resistance, and ratiometric fluorescence technique was adopted to achieve visual signal output by doping green CdTe QDs into silica shell...
April 1, 2017: Talanta
https://www.readbyqxmd.com/read/28149023/status-of-serum-calcium-vitamin-d-and-parathyroid-hormone-and-hematological-indices-among-lead-exposed-jewelry-workers-in-dhaka-bangladesh
#12
I Mazumdar, K Goswami, Md Suhrab Ali
Jewelry utilizes lead either directly or as a base metal. Costume jewelry requires lead before molding and plating the product with valuable metals. Therefore, such ornaments have a great potential to release heavy metals having health hazards. Also, jewelry makers engaged in preparing German silver, an alloy, apply lead in smelting, alloying, rolling and milling silver wires and pieces. The metal is taken up by blood, soft tissues and bone. The biological effects of lead are dependent upon the level and duration of exposure...
March 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/28131027/development-of-a-new-hplc-based-method-for-3-nitrotyrosine-quantification-in-different-biological-matrices
#13
Dulce Teixeira, Cristina Prudêncio, Mónica Vieira
BACKGROUND: The nitration of tyrosine residues in proteins is associated with nitrosative stress, resulting in the formation of 3-nitrotyrosine (3-NT).(1) 3-NT levels in biological samples have been associated with numerous physiological and pathological conditions. Hence several attempts have been made in order to develop methods that accurately quantify 3-NT in these matrices. The aim of this study was to develop a simple, rapid, low-cost and sensitive high-performance liquid chromatography (HPLC)-based 3-NT quantification method...
January 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28129394/characterization-of-early-phase-neutrophil-extracellular-traps-in-urinary-tract-infections
#14
Yanbao Yu, Keehwan Kwon, Tamara Tsitrin, Shiferaw Bekele, Patricia Sikorski, Karen E Nelson, Rembert Pieper
Neutrophils have an important role in the antimicrobial defense and resolution of urinary tract infections (UTIs). Our research suggests that a mechanism known as neutrophil extracellular trap (NET) formation is a defense strategy to combat pathogens that have invaded the urinary tract. A set of human urine specimens with very high neutrophil counts had microscopic evidence of cellular aggregation and lysis. Deoxyribonuclease I (DNase) treatment resulted in disaggregation of such structures, release of DNA fragments and a proteome enriched in histones and azurophilic granule effectors whose quantitative composition was similar to that of previously described in vitro-formed NETs...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28115749/chemokines-in-tumor-proximal-fluids
#15
Jaromir Kotyza
Chemokines are chemotactic cytokines produced by leukocytes and other types of cells including tumor cells. Their action is determined by the expression of cognate receptors and subsequent signaling in target cells, followed by the modulation of cytoskeletal proteins and the induction of other responses. In tumors, chemokines produced by neoplastic/stroma cells control the leukocyte infiltrate influencing tumor growth and progression. Tumor cells also express functional chemokine receptors responding to chemokine signals, promoting cell survival, proliferation and metastasis formation...
January 6, 2017: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/28112564/frequency-clinicopathological-features-and-phylogenetic-analysis-of-feline-morbillivirus-in-cats-in-istanbul-turkey
#16
Huseyin Yilmaz, Bilge K Tekelioglu, Aydin Gurel, Ozge E Bamac, Gulay Y Ozturk, Utku Y Cizmecigil, Eda A Tarakci, Ozge Aydin, Aysun Yilmaz, Eduardo Berriatua, Chris R Helps, Juergen A Richt, Nuri Turan
Objectives The aim of the study was to investigate feline morbillivirus (FmoPV) frequency, phylogeny and associated pathology in cats in Istanbul, Turkey. Methods Samples from sick (n = 96) and dead ( n = 15) cats were analysed using reverse transcription PCR. Blood and urine analyses and histopathology were also performed. Results FmoPV RNA was detected in six cats (5.4%), including three sick (in the urine) and three dead cats (tissues). A significantly greater proportion of FmoPV RNA-positive cats had street access compared with non-infected cats...
January 1, 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28110124/monoanionic-99m-tc-tricarbonyl-aminopolycarboxylate-complexes-with-uncharged-pendant-groups-radiosynthesis-and-evaluation-as-potential-renal-tubular-tracers
#17
Malgorzata Lipowska, Jeffrey Klenc, Nashwa Jarkas, Luigi G Marzilli, Andrew T Taylor
INTRODUCTION: (99m)Tc(CO)3-nitrilotriacetic acid, (99m)Tc(CO)3(NTA), is a new renal tubular agent with pharmacokinetic properties comparable to those of (131)I-OIH but the clearance of (99m)Tc(CO)3(NTA) and (131)I-OIH is still less than the clearance of PAH, the gold standard for the measurement of effective renal plasma flow. At physiological pH, dianionic (99m)Tc(CO)3(NTA) has a mononegative inner metal-coordination sphere and a mononegative uncoordinated carboxyl group. To evaluate alternate synthetic approaches, we assessed the importance of an uncoordinated carboxyl group, long considered essential for tubular transport, by evaluating the pharmacokinetics of three analogs with the (99m)Tc(CO)3(NTA) metal-coordination sphere but with uncharged pendant groups...
December 27, 2016: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/28109429/body-fluid-degradomics-and-characterization-of-basic-n-terminome
#18
F Sabino, O Hermes, U Auf dem Keller
Rapid improvements in instrumentation and data analysis make mass spectrometry-based proteomics the method of choice for global characterization of proteomes and discovery of protein-based biomarkers. On the contrary to tissue biopsies, body fluids-e.g., blood, wound fluid, urine, and saliva-are noninvasive and easy to collect and process. However, they are very complex and present high dynamic ranges of protein concentrations, rendering direct shotgun proteomics analysis as inefficient for identification of low-abundance proteins in these specimens...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28108355/an-unusual-case-of-a-pheochromocytoma-mimicking-both-acute-coronary-syndrome-and-central-nervous-system-infection-case-report-and-literature-review
#19
Andreas Mitsis, Christina Hadjilouka, Maria Skarpari, Michael Myrianthefs
Pheochromocytoma has been called 'the great mimic', capable of presenting itself as many other medical conditions. We present a 58-year-old woman admitted with recurrent episodes of nausea and vomiting and high blood pressure. Elevated cardiac biomarkers and diffuse ST-T abnormalities on electrocardiography suggested myocardial injury. However the biochemical and electrocardiography abnormalities normalized in a few hours. The next day the combination of fever, mental disorientation and confusion associated with elevated levels of C-reactive protein and leukocytosis suggested infection of central neural system...
January 17, 2017: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/28104821/the-urine-preservative-acetic-acid-degrades-urine-protein-implications-for-urine-biorepositories-and-the-aask-cohort-study
#20
Salem Almaani, Lee A Hebert, Brad H Rovin, Daniel J Birmingham
Patients enrolled in the African American Study of Kidney Disease and Hypertension (AASK) Cohort Study who exhibited overt proteinuria have been reported to show high nonalbumin proteinuria (NAP), which is characteristic of a tubulopathy. To determine whether African American Study of Kidney Disease and Hypertension nephropathy (AASK-N) is a tubulopathy, we obtained urine samples of 37 patients with AASK-N, with 24-hour protein-to-creatinine ratios (milligrams per milligram) ranging from 0.2 to 1.0, from the National Institute of Diabetes and Digestive Kidney Diseases repository and tested for seven markers of tubular proteinuria...
January 19, 2017: Journal of the American Society of Nephrology: JASN
keyword
keyword
24208
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"