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High protein in urine

Yong Li, Peggy Sekula, Matthias Wuttke, Judith Wahrheit, Birgit Hausknecht, Ulla T Schultheiss, Wolfram Gronwald, Pascal Schlosser, Sara Tucci, Arif B Ekici, Ute Spiekerkoetter, Florian Kronenberg, Kai-Uwe Eckardt, Peter J Oefner, Anna Köttgen
Background The kidneys have a central role in the generation, turnover, transport, and excretion of metabolites, and these functions can be altered in CKD. Genetic studies of metabolite concentrations can identify proteins performing these functions. Methods We conducted genome-wide association studies and aggregate rare variant tests of the concentrations of 139 serum metabolites and 41 urine metabolites, as well as their pairwise ratios and fractional excretions in up to 1168 patients with CKD. Results After correction for multiple testing, genome-wide significant associations were detected for 25 serum metabolites, two urine metabolites, and 259 serum and 14 urinary metabolite ratios...
March 15, 2018: Journal of the American Society of Nephrology: JASN
Marianna Caterino, Miriam Zacchia, Michele Costanzo, Giuliana Bruno, Davide Arcaniolo, Francesco Trepiccione, Rosa Anna Siciliano, Maria Fiorella Mazzeo, Margherita Ruoppolo, Giovambattista Capasso
BACKGROUND: /Aims: Renal disease is a common cause of morbidity in patients with Bardet-Biedl syndrome (BBS), however the severity of kidney dysfunction is highly variable. To date, there is little information on the pathogenesis, the risk and predictor factors for poor renal outcome in this setting. The present study aims to analyze the spectrum of urinary proteins in BBS patients, in order to potentially identify 1) disease-specific proteomic profiles that may differentiate the patients from normal subjects; 2) urinary markers of renal dysfunction...
March 8, 2018: Kidney & Blood Pressure Research
Yue Yang, Long Chen, Yuan Jia, Yang Liu, Lei Wen, Yaoxian Liang, Yuan An, Shi Chen, Yin Su, Zhanguo Li
To analyze the clinical spectrum, laboratory characteristics, and outcomes of monoclonal gammopathy (MG) in patients with rheumatic diseases. Screening for the presence of MG was performed in 872 inpatients with rheumatic diseases from January 2010 to July 2017. A total of 41 patients were enrolled. Their clinical and biological features in addition to outcomes were described. For each patient with primary Sjögren syndrome (pSS), 2 age- and sex-matched pSS patients without MG were selected as controls. Risk factors for the presence of MG and malignant hematological neoplasias were assessed...
March 13, 2018: Clinical Rheumatology
Mindi Zhao, Jianqiang Wu, Xundou Li, Youhe Gao
Urine is a better source than plasma for biomarker studies, as it can accumulate all changes in the body. Various candidate urinary biomarkers of physiological condition, kidney disease and even brain dysfunction, have been detected in urine; however, urine has rarely been used to reflect cardiac diseases. In this study, urine at day 0, 14, 21 and 28 were collected from the myosin-induced autoimmune myocarditis rat models. The candidate urinary biomarkers were then characterized using the isobaric tandem mass tag labeling approach coupled with offline two-dimensional reverse-phase liquid chromatography and high-resolution mass spectrometry...
March 7, 2018: Journal of Proteomics
Jing Xu, Hong Xu, Yang Yu, Yi He, Qi Liu, Bo Yang
BACKGROUND The purpose of the present study was to assess the effect of luteolin and solifenacin on diabetic cystopathy (DCP) and to investigate the mechanism of action. A novel link between the overexpression of c-Kit in the bladder and voiding dysfunction was identified in rats with DCP. MATERIAL AND METHODS A rat model of DCP was successfully established by intraperitoneal injection of streptozotocin and a diet high in glucose and lipids, and animals were treated with luteolin and solifenacin. The effect of luteolin and solifenacin on urinary dysfunction in DCP rats was investigated by assessing bladder pressure and performing a volume test...
March 10, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Yasushi Kaburagi, Eri Takahashi, Hiroshi Kajio, Shigeo Yamashita, Ritsuko Yamamoto-Honda, Tomoko Shiga, Akinori Okumura, Atsushi Goto, Yuka Fukazawa, Naoto Seki, Kazuyuki Tobe, Michihiro Matsumoto, Mitsuhiko Noda, Hiroyuki Unoki-Kubota
AIMS: In this study, we applied quantitative proteomic analysis to identify urinary proteins associated with diabetic nephropathy (DN). METHODS: Two-dimensional image-converted analysis of liquid chromatography and mass spectrometry detected the proteins differentially excreted between normoalbuminuric and macroalbuminuric patients with type 2 diabetes mellitus (T2DM) (n = 6 each). Urinary levels of excreted proteins were measured by multiple reaction monitoring (MRM) analysis using an independent sample set (n = 77)...
March 6, 2018: Diabetes Research and Clinical Practice
Tao Ding, Shaofei Wang, Xuyao Zhang, Wenjing Zai, Jiajun Fan, Wei Chen, Qi Bian, Jingyun Luan, Yilan Shen, Yanda Zhang, Dianwen Ju, Xiaobin Mei
BACKGROUND: Diabetic nephropathy (DN), the leading cause of end-stage renal disease, is acknowledged as an independent risk factor for cardiovascular disease, which underlines the urgent need for new medications to DN. Dihydroquercetin (DHQ), an important natural dihydroflavone, exerts significant antioxidant, anti-inflammatory, and antifibrotic properties, but its effects on DN have not been investigated yet. PURPOSE: We aimed to explore the kidney protection effects of DHQ on DN rats induced by high-fat diet/streptozotocin in vivo and the underlying mechanisms of DHQ on renal cells including HBZY-1 and HK2 exposed to high glucose in vitro...
March 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Gaoli He, Beining Guo, Jing Zhang, Yi Li, Xiaojie Wu, Yaxin Fan, Yuancheng Chen, Guoying Cao, Jicheng Yu
Levornidazole is a novel third-generation nitroimidazoles antibiotic which metabolism and disposition in human are not well known. We have previously developed two methods to quantify levornidazole and its phase I metabolites, Ml (Hydroxylation metabolite), M2 (N-dealkylation metabolite) and M4 (Oxidative dechlorination metabolite), in human plasma and urine. In this study, we developed three novel liquid chromatographic-tandem mass spectrometric (LC-MS/MS) methods and analyzed its phase II metabolites, sulfate conjugate (M6) and glucuronide conjugate (M16), in human plasma and urine, and the parent drug and above-mentioned five metabolites in human feces samples...
February 23, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Lauri-Anne McDermott, Marie H Murphy, Andrea M McNeilly, Jane P Rankin, Jackie H Gracey
The number of people living with and beyond cancer is at an all time high. These survivors are not necessarily living well, as adverse side effects from cancer and its treatment can last up to 5 years and leave patients at a higher risk of developing secondary cancers and other chronic illnesses. Exercise has been proven to be a safe and effective method of intervention to decrease mortality and overall improve health outcomes. The biological mechanism through which this occurs is an area of research that is in its infancy and not well defined...
January 2018: Journal of Cancer Research and Therapeutics
Alexandra Kovalčíková, Marianna Gyurászová, Diana Vavrincová-Yaghi, Peter Vavrinec, Ľubomíra Tóthová, Peter Boor, Katarína Šebeková, Peter Celec
Uremic encephalopathy is a severe complication of renal failure. The underlying pathogenesis is unknown although several mechanisms have been suggested. Renal failure causes oxidative stress leading to cardiovascular complications. It has been suggested as the potential mediator of uremic encephalopathy as well, but it is largely unknown whether brain tissue itself undergoes oxidative damage in uremia. The aim of our experiment was to analyze oxidative stress markers in different brain regions in an animal model of acute kidney injury (AKI)...
March 7, 2018: Metabolic Brain Disease
Jason H Greenberg, Prasad Devarajan, Heather R Thiessen-Philbrook, Catherine Krawczeski, Chirag R Parikh, Michael Zappitelli
BACKGROUND: We previously reported that children undergoing cardiac surgery are at high risk for long-term chronic kidney disease (CKD) and hypertension, although postoperative acute kidney injury (AKI) is not a risk factor for worse long-term kidney outcomes. We report here our evaluation of renal injury biomarkers 5 years after cardiac surgery to determine whether they are associated with postoperative AKI or long-term CKD and hypertension. METHODS: Children aged 1 month to 18 years old undergoing cardiopulmonary bypass were recruited to this prospective cohort study...
March 6, 2018: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
Hee Mang Yoon, Jung Heon Kim, Jong Seung Lee, Jeong-Min Ryu, Dae Yeon Kim, Jeong-Yong Lee
BACKGROUND: Appendicolith can cause appendiceal obstruction and acute appendicitis. Its high prevalence may be related to the high perforation rate in pediatric appendicitis. This study assessed the characteristics of appendicolith and its clinical significance in pediatric appendicitis. METHODS: A retrospective study was performed among children and adolescents younger than 17 years who were preoperatively diagnosed with appendicitis in the pediatric emergency department (ED)...
March 5, 2018: World Journal of Pediatrics: WJP
Felix C F Schmitt, Eduardo Salgado, Janina Friebe, Thomas Schmoch, Florian Uhle, Thomas Fleming, Johanna Zemva, Lars Kihm, Christian Nusshag, Christian Morath, Martin Zeier, Thomas Bruckner, Arianeb Mehrabi, Peter P Nawroth, Markus A Weigand, Stefan Hofer, Thorsten Brenner
BACKGROUND: A prolonged cold ischemia time (CIT) is suspected to be associated with an increased ischemia and reperfusion injury (IRI) resulting in an increased damage to the graft. METHODS: In total, 91 patients were evaluated for a delayed graft function within 7 days after kidney transplantation (48 deceased, 43 living donors). Blood and urine samples were collected before, immediately after the operation, and 1, 3, 5, 7 and 10 days later. Plasma and/or urine levels of total keratin 18 (total K18), caspase-cleaved keratin 18 (cc K18), the soluble receptor for advanced glycation end-products (sRAGE), tissue inhibitor of metalloproteinase-2 and insulin-like growth factor-binding protein-7 ([TIMP-2]×[IGFBP7]) were measured...
March 5, 2018: Transplant International: Official Journal of the European Society for Organ Transplantation
Alimujiang Abulimiti, Aierhati Husaiyin, Yalikun Sailai
Systemic inflammatory response syndrome (SIRS) prevention is key to severe acute pancreatitis (SAP) treatment and the assessment of high-volume hemofiltration (HVHF) for treating SAP accompanying multiple organ dysfunction syndromes.In this prospective controlled study, 40 SAP patients were divided into 2 groups: control (n = 22, treated with fasting, decompression, and intravenous somatostatin) and HVHF (n = 18, HVHF administration in addition to the treatment in the control group) groups; and were assessed for serum and urine amylase, WBC, C-reactive protein (CRP), and hepatic and renal functions...
January 2018: Medicine (Baltimore)
Ali Hashemi Gheinani, Mike Vögeli, Ulrich Baumgartner, Erik Vassella, Annette Draeger, Fiona C Burkhard, Katia Monastyrskaya
Circulating miRNAs are detected in extracellular space and body fluids such as urine. Circulating RNAs can be packaged in secreted urinary extracellular vesicles (uEVs) and thus protected from degradation. Urinary exosome preparations might contain specific miRNAs, relevant as biomarkers in renal and bladder diseases. Major difficulties in application of uEVs into the clinical environment are the high variability and low reproducibility of uEV isolation methods. Here we used five different methods to isolate uEVs and compared the size distribution, morphology, yield, presence of exosomal protein markers and RNA content of uEVs...
March 2, 2018: Scientific Reports
Yan-Ping Guo, Hong-Kun Jiang, Hong Jiang, Hong-Yu Tian, Lei Li
OBJECTIVES: To investigate the effects of lipoxin A4 (LXA4) on inflammatory responses in obesity-related glomerulopathy (ORG) mouse model and its potential mechanisms. METHODS: Male C57BL/6 mice were randomly divided into 4 groups: normal, model, LXA4, and LXA4/Boc-2 groups (n = 8). Mice in LXA4 group were intraperitoneally injected with LXA4 (40 ng/kg) once daily for 3 days following 12 weeks of high-fat diet (HFD) feeding. LXA4 receptor antagonist, Boc-2, was administered in LXA4/Boc-2 group prior to LXA4 treatment to block the effects of LXA4...
February 27, 2018: Life Sciences
Sarah Trame, Inga Wessels, Hajo Haase, Lothar Rink
Inadequate dietary zinc intake is wide-spread in the world's population. Despite the clinical significance of zinc deficiency there is no established method or biomarker to reliably evaluate the zinc status. The aim of our study was to develop a biochemically validated questionnaire as a clinically useful tool that can predict the risk of an individual being zinc deficient. From 71 subjects aged 18-55 years blood and urine samples were collected. Zinc concentrations in serum and urine were determined by atomic absorption spectrometry...
February 21, 2018: Journal of Trace Elements in Medicine and Biology
Liang Li, Feng-Hua Sun, Wendy Ya-Jun Huang, Stephen Heung-Sang Wong
The purpose of this study was to examine the effects of different amounts of whey protein in carbohydrate-electrolyte (CE) drinks on post-exercise rehydration. Ten males completed 5 trials in a randomised cross-over design. A 4-h recovery was applied after a 60-min run at 65% VO2peak in each trial. During recovery, the participants ingested a high-carbohydrate CE drink (CE-H), a low-carbohydrate CE drink (CE-L), a high-whey-protein (33 g·L-1 ) CE drink (CW-H), a medium-whey-protein (22 g·L-1 ) CE drink (CW-M) or a low-whey-protein (15 g·L-1 ) CE drink (CW-L) in a volume equivalent to 150% of their body mass (BM) loss...
March 1, 2018: European Journal of Sport Science
G O Oludare, O J Ilo, B A Lamidi
This study investigated the effect of high salt water intake and lipopolysaccharide injection as probable rat models of preeclampsia during pregnancy. Thirty-three female Sprague-Dawley rats weighing between 150-170g were divided into 4 groups of the control (normal saline), high salt water intake (1.8% NaCl from days 13-18 of pregnancy), lipopolysaccharide group (40 µg/kg b.w. ip injection from day 16-18 of pregnancy) and high salt water (1.8% NaCl) and lipopolysaccharide injection (40 µg/kg b.w i.p. from days 16-18 of pregnancy)...
December 30, 2017: Nigerian Journal of Physiological Sciences: Official Publication of the Physiological Society of Nigeria
Attia Anwar, Provvidenza Maria Abruzzo, Sabah Pasha, Kashif Rajpoot, Alessandra Bolotta, Alessandro Ghezzo, Marina Marini, Annio Posar, Paola Visconti, Paul J Thornalley, Naila Rabbani
Background: Clinical chemistry tests for autism spectrum disorder (ASD) are currently unavailable. The aim of this study was to explore the diagnostic utility of proteotoxic biomarkers in plasma and urine, plasma protein glycation, oxidation, and nitration adducts, and related glycated, oxidized, and nitrated amino acids (free adducts), for the clinical diagnosis of ASD. Methods: Thirty-eight children with ASD (29 male, 9 female; age 7.6 ± 2.0 years) and 31 age-matched healthy controls (23 males, 8 females; 8...
2018: Molecular Autism
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