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https://www.readbyqxmd.com/read/100001003/emergency-management-of-sepsis-the-simple-stuff-saves-lives
#1
David Sweet, Julian Marsden, Kendall Ho, Christina Krause, James A Russell
Many emergency departments have implemented sepsis protocols since the 2001 publication of results from the early goal-directed therapy trial, which showed early targeted resuscitation lowers mortality. As part of an attempt to improve clinical and operational outcomes for emergency departments across British Columbia, we reviewed sepsis management literature and considered sepsis protocol implementation in the province’s emergency departments. During the literature review we found that many observational studies confirmed an association between implementation of emergency sepsis protocols and decreased mortality...
May 2012: BC medical journal: BCMJ
https://www.readbyqxmd.com/read/28214999/e6-associated-protein-dependent-estrogen-receptor-regulation-of-protein-kinase-a-regulatory-subunit-r2a-expression-in-neuroblastoma
#2
Jean-Pierre Obeid, Youssef H Zeidan, Nawal Zafar, Jimmy El Hokayem
E6ap is a known transcriptional coregulator for estrogen receptor alpha (Er, Erα) in the presence of estrogen. Protein kinase A (PKA) contains two regulatory subunits derived from four genes. Recent evidence demonstrates that PKA regulates E6ap activity. Data generated in our lab indicated estrogen dependent regulation of Pkar2a levels. Our project sets to investigate a possible feedback mechanism constituting of Erα and E6ap transcriptional regulation of Pkar2a expression. Western blot evaluated protein regulation correlations with E2 in mouse neuroblastoma lines...
February 18, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28214975/surveillance-scans-in-lymphoma-friend-or-foe
#3
REVIEW
Tycel Phillips, Jessica Mercer
Advancements in the treatment of lymphoma over the last few decades have allowed more patients to achieve a remission after the completion of therapy. Due to the improvement in response rates, methods to detect recurrence early and accurately during follow-up, especially in patients with potential curable aggressive lymphomas, are a key. Observation has always involved close clinical follow-up with the use of physical exams and routine labs, but rapid changes in technology have allowed CT scans, PET scans, and MRIs to become an integral part of managing patients with lymphoma...
February 2017: Current Treatment Options in Oncology
https://www.readbyqxmd.com/read/28214546/effect-of-cyclophosphamide-on-the-solid-form-of-mannitol-during-lyophilization
#4
Krupaliben Patel, Bhushan Munjal, Arvind K Bansal
Mannitol is a commonly used bulking agent in lyophilized formulations. It can crystallize into multiple solid forms during lyophilization thereby exhibiting phase heterogeneity and variability in product performance. In this manuscript, we studied the effect of cyclophosphamide (CPA), an anticancer drug, on the solid form of mannitol during lyophilization from aqueous solutions. Freeze-concentration studies were performed in the DSC while lyophilization was performed in a lab scale freeze dryer. DSC experiments revealed two-stage crystallization of mannitol (1...
February 15, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28214447/influence-of-pressures-up-to-50bar-on-two-stage-anaerobic-digestion
#5
Wolfgang Merkle, Katharina Baer, Jonas Lindner, Simon Zielonka, Felix Ortloff, Frank Graf, Thomas Kolb, Thomas Jungbluth, Andreas Lemmer
The concept of pressurized two-stage anaerobic digestion integrates biogas production, purification and pressure boosting within one process. The produced methane-rich biogas can be fed into gas grids with considerably less purification effort. To investigate biogas production under high pressures up to 50bar, a lab scale two-stage anaerobic digestion system was constructed including one continuously operated pressurized methane reactor. This investigation examined the effects of different operating pressures in methane reactor (10, 25, 50bar) on biogas quantity and quality, pH value and process stability...
February 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28214369/diagnostic-accuracy-of-history-physical-exam-laboratory-tests-and-point-of-care-ultrasound-for-pediatric-acute-appendicitis-in-the-emergency-department-a-systematic-review-and-meta-analysis
#6
Roshanak Benabbas, Mark Hanna, Jay Shah, Richard Sinert
BACKGROUND: Acute appendicitis (AA) is the most common surgical emergency in children. Accurate and timely diagnosis is crucial but challenging due to atypical presentations and the inherent difficulty of obtaining a reliable history and physical examination in younger children. OBJECTIVES: To determine the utility of history, physical exam, laboratory tests, Pediatric Appendicitis Score (PAS) and Emergency Department-Point-of-Care Ultrasound (ED-POCUS) in the diagnosis of AA in ED pediatric patients...
February 18, 2017: Academic Emergency Medicine: Official Journal of the Society for Academic Emergency Medicine
https://www.readbyqxmd.com/read/28214331/pomc-maintains-tumor-initiating-properties-of-tumor-tissue-derived-long-term-cultured-breast-cancer-stem-cells
#7
Xiaoti Lin, Weiyu Chen, Fengqin Wei, Binhua P Zhou, Mien-Chie Hung, Xiaoming Xie
The identification and understanding of the molecular network of cancer stem cells (CSCs) have had a profound impact on our view of carcinogenesis and treatment strategy. Unfortunately, a major problem is that serial passages of CSCs from clinical solid tumor specimens currently are not available in any lab, and thus, reported data are difficult to confirm and intensively interrogated. Here, we have generated two tumor tissue-derived breast CSC (BCSC) lines that showed prolonged maintenance over 20 serial passages in vitro, while retaining their tumor-initiating biological properties...
February 18, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28213985/capillary-dynamics-driven-by-molecular-self-layering
#8
Pingkeng Wu, Alex Nikolov, Darsh Wasan
Capillary dynamics is a ubiquitous everyday phenomenon. It has practical applications in diverse fields, including ink-jet printing, lab-on-a-chip, biotechnology, and coating. Understanding capillary dynamics requires essential knowledge on the molecular level of how fluid molecules interact with a solid substrate (the wall). Recent studies conducted with the surface force apparatus (SFA), atomic force microscope (AFM), and statistical mechanics simulation revealed that molecules/nanoparticles confined into the film/wall surfaces tend to self-layer into 2D layer/s and even 2D in-layer with increased confinement and fluid volume fraction...
February 10, 2017: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/28213975/lab-in-a-tube-real-time-molecular-point-of-care-diagnostics-for-influenza-a-and-b-using-the-cobas%C3%A2-liat%C3%A2-system
#9
Willem Jg Melchers, Judith Kuijpers, Joanna Sickler, Janette Rahamat-Langendoen
BACKGROUND: Rapid diagnosis of influenza A and B is important for direct treatment decisions in patient care and for the reduction of in-hospital transmissions. The new real-time PCR based molecular point-of-care (POC) assay, the cobas(®) Influenza A/B test on the cobas(®) Liat® System (cobas(®) Liat® Influenza A/B assay), generated a PCR result in less than 20 minutes, was evaluated for the detection of influenza A and B. METHOD OF THE STUDY: 121 retrospectively collected respiratory specimens, previously analyzed with a routine influenza A/B test (Diagenode) were tested using the cobas(®) Liat® Influenza A/B assay...
February 18, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28213732/the-potential-of-lactic-acid-bacteria-to-colonize-biotic-and-abiotic-surfaces-and-the-investigation-of-their-interactions-and-mechanisms
#10
REVIEW
Mattia Pia Arena, Vittorio Capozzi, Giuseppe Spano, Daniela Fiocco
Lactic acid bacteria (LAB) are a heterogeneous group of Gram-positive bacteria that comprise several species which have evolved in close association with humans (food and lifestyle). While their use to ferment food dates back to very ancient times, in the last decades, LAB have attracted much attention for their documented beneficial properties and for potential biomedical applications. Some LAB are commensal that colonize, stably or transiently, host mucosal surfaces, inlcuding the gut, where they may contribute to host health...
February 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28213547/laboratory-selection-and-characterization-of-resistance-to-the-bacillus-thuringiensis-vip3aa-toxin-in-heliothis-virescens-lepidoptera-noctuidae
#11
Brian R Pickett, Asim Gulzar, Juan Ferré, Denis J Wright
Laboratory selection with Vip3Aa of a field-derived population of Heliothis virescens produced >2040-fold resistance in 12 generations of selection. The Vip-Sel resistant population showed little cross-resistance to Cry1Ab and no cross-resistance to Cry1Ac. Resistance was unstable after 15 generations without exposure to the toxin. F1 reciprocal crosses between Vip-Unsel and Vip-Sel indicated a strong paternal influence on the inheritance of resistance. Resistance ranged from almost completely recessive (mean h = 0...
February 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28213342/virtual-visits-for-acute-nonurgent-care-a-claims-analysis-of-episode-level-utilization
#12
Aliza S Gordon, Wallace C Adamson, Andrea R DeVries
BACKGROUND: Expansion of virtual health care-real-time video consultation with a physician via the Internet-will continue as use of mobile devices and patient demand for immediate, convenient access to care grow. OBJECTIVE: The objective of the study is to analyze the care provided and the cost of virtual visits over a 3-week episode compared with in-person visits to retail health clinics (RHC), urgent care centers (UCC), emergency departments (ED), or primary care physicians (PCP) for acute, nonurgent conditions...
February 17, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/28213247/an-automatic-stirring-assisted-liquid-liquid-microextraction-system-based-on-lab-in-syringe-platform-for-on-line-atomic-spectrometric-determination-of-trace-metals
#13
Georgia Giakisikli, Aristidis N Anthemidis
A novel simple fully automatic on-line magnetic stirring-assisted liquid-liquid microextraction method, based on the lab-in-syringe (LIS) concept, has been developed as an alternative approach for sample pretreatment and atomic spectrometric assays. The analytical process includes the in-syringe reaction of the metal ion with the chelating reagent, the analyte micro-extraction and the subsequent transportation of the extractant to the detection system for electrothermal atomic absorption spectrometric (ETAAS) quantification...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213032/applicability-of-a-colorimetric-method-for-evaluation-of-lactic-acid-bacteria-with-probiotic-properties
#14
Min Young Jung, Jina Lee, Boyeon Park, Hyelyeon Hwang, Sung-Oh Sohn, Se Hee Lee, Hyeong In Lim, Hae Woong Park, Jong-Hee Lee
Rapid colorimetric methods using various indicator reagents have been developed to monitor bacterial viability. Here, we examined the applicability of a method based on the reduction of resazurin or water-soluble tetrazolium salt-8 (WST-8) to screen lactic acid bacteria (LAB) for growth, tolerance against bile acid and low pH. The resazurin reduction test proved unsuitable for screening LAB such as Lactobacillus plantarum and Leuconostoc mesenteroides since it reacted with acid present in the cultures. LAB growth could be indirectly quantified by measuring WST-8 reduction...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28212875/genotyping-by-sequencing-provides-the-first-well-resolved-phylogeny-for-coffee-coffea-and-insights-into-the-evolution-of-caffeine-content-in-its-species-gbs-coffee-phylogeny-and-the-evolution-of-caffeine-content
#15
Perla Hamon, Corrinne E Grover, Aaron P Davis, Jean-Jacques Rakotomalala, Nathalie E Raharimalala, Victor A Albert, Hosahalli L Sreenath, Piet Stoffelen, Sharon E Mitchell, Emmanuel Couturon, Serge Hamon, Alexandre de Kochko, Dominique Crouzillat, Michel Rigoreau, Ucu Sumirat, Sélastique Akaffou, Romain Guyot
A comprehensive and meaningful phylogenetic hypothesis for the commercially important coffee genus (Coffea) has long been a key objective for coffee researchers. For molecular studies, progress has been limited by low levels of sequence divergence, leading to insufficient topological resolution and statistical support in phylogenetic trees, particularly for the major lineages and for the numerous species occurring in Madagascar. We report here the first almost fully resolved, broadly sampled phylogenetic hypothesis for coffee, the result of combining genotyping-by-sequencing (GBS) technology with a newly developed, lab-based workflow to integrate short read next-generation sequencing for low numbers of additional samples...
February 14, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28212510/impact-of-paint-shop-decanter-effluents-on-biological-treatability-of-automotive-industry-wastewater
#16
Didem Güven, Oytun Hanhan, Elif Ceren Aksoy, Güçlü Insel, Emine Çokgör
A lab-scale Sequencing Batch Reactor (SBR) was implemented to investigate biological treatability and kinetic characteristics of paint shop wastewater (PSW) together with main stream wastewater (MSW) of a bus production factory. Readily biodegradable and slowly biodegradable COD fractions of MWS were determined by respirometric analysis: 4.2% (SS), 10.4% (SH) and 59.3% (XS). Carbon and nitrogen removal performance of the SBR feeding with MSW alone were obtained as 89% and 58%, respectively. When PSW was introduced to MSW, both carbon and nitrogen removal were deteriorated...
January 29, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28212390/lack-of-effects-of-fish-oil-supplementation-for-12-weeks-on-resting-metabolic-rate-and-substrate-oxidation-in-healthy-young-men-a-randomized-controlled-trial
#17
Sebastian Jannas-Vela, Kaitlin Roke, Stephanie Boville, David M Mutch, Lawrence L Spriet
: Fish oil (FO) has been shown to have beneficial effects in the body via incorporation into the membranes of many tissues. It has been proposed that omega-3 fatty acids in FO may increase whole body resting metabolic rate (RMR) and fatty acid (FA) oxidation in human subjects, but the results to date are equivocal. The purpose of this study was to investigate the effects of a 12 week FO supplementation period on RMR and substrate oxidation, in comparison to an olive oil (OO) control group, in young healthy males (n = 26; 22...
2017: PloS One
https://www.readbyqxmd.com/read/28212302/the-importance-of-conditioned-stimuli-in-cigarette-and-e-cigarette-craving-reduction-by-e-cigarettes
#18
Martijn Van Heel, Dinska Van Gucht, Koen Vanbrabant, Frank Baeyens
This study examined the impact of four variables pertaining to the use of e-cigarettes (e-cigs) on cravings for tobacco cigarettes and for e-cigs after an overnight abstinence period. The four variables were the nicotine level, the sensorimotor component, the visual aspect, and the aroma of the e-cig. In an experimental study, 81 participants without prior vaping experience first got acquainted with using e-cigs in a one-week tryout period, after which they participated in a lab session assessing the effect of five minutes of vaping following an abstinence period of 12 h...
February 15, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28212019/complete-nutrient-removal-coupled-to-nitrous-oxide-production-as-a-bioenergy-source-by-denitrifying-polyphosphate-accumulating-organisms
#19
Han Gao, Miaomiao Liu, James S Griffin, Longcheng Xu, Da Xiang, Yaniv D Scherson, Wen-Tso Liu, George F Wells
Coupled Aerobic-anoxic Nitrous Decomposition Operation (CANDO) is a promising emerging bioprocess for wastewater treatment that enables direct energy recovery from nitrogen (N) in three steps: (1) ammonium oxidation to nitrite; (2) denitrification of nitrite to nitrous oxide (N2O); and (3) N2O conversion to N2 with energy generation. However, CANDO does not currently target phosphorus (P) removal. Here, we demonstrate that denitrifying polyphosphate accumulating organism (PAO) enrichment cultures are capable of catalyzing simultaneous biological N and P removal coupled to N2O generation in a 2nd generation CANDO process, CANDO+P...
February 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28211993/recent-lab-on-chip-developments-for-novel-drug-discovery
#20
REVIEW
Nauman Khalid, Isao Kobayashi, Mitsutoshi Nakajima
Microelectromechanical systems (MEMS) and micro total analysis systems (μTAS) revolutionized the biochemical and electronic industries, and this miniaturization process became a key driver for many markets. Now, it is a driving force for innovations in life sciences, diagnostics, analytical sciences, and chemistry, which are called 'lab-on-a-chip, (LOC)' devices. The use of these devices allows the development of fast, portable, and easy-to-use systems with a high level of functional integration for applications such as point-of-care diagnostics, forensics, the analysis of biomolecules, environmental or food analysis, and drug development...
February 17, 2017: Wiley Interdisciplinary Reviews. Systems Biology and Medicine
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