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Scaffold rapid prototyping

Tingfang Sun, Kui Zhou, Man Liu, Xiaodong Guo, Yanzhen Qu, Wei Cui, ZengWu Shao, Xianglin Zhang, Shuyun Xu
Extrusion free-forming, as a rapid prototyping technique, is extensively applied in fabricating ceramic material in bone tissue engineering. To improve the osteoinductivity of nano-hydroxyapatite (nHA) scaffold fabricated by extrusion free-forming, in this study, we incorporated a new peptide (P28) and optimized the superficial microstructure after shaping by controlling the sintering temperature. P28, a novel bone morphogenic protein 2 (BMP-2)-related peptide, was designed in this study. Analysis of the structure, physicochemical properties, and release kinetics of P28 from nHA sintered at temperatures ranging from 1000 °C to 1400 °C revealed that nHA sintered at 1000 °C had higher porosity, preferable pore size, and better capacity to control P28 release than that sintered at other temperatures...
November 25, 2016: Journal of Tissue Engineering and Regenerative Medicine
Ting Guo, Josephine Lembong, Lijie Grace Zhang, John P Fisher
In the past few decades, the field of tissue engineering combined with rapid prototyping (RP) techniques has been successful in creating biological substitutes that mimic tissues. Its applications in regenerative medicine have drawn efforts in research from various scientific fields, diagnostics, and clinical translation to therapies. While some areas of therapeutics are well developed, such as skin replacement, many others such as cartilage repair can still greatly benefit from tissue engineering and RP due to the low success and / or inefficiency of current existing, often surgical treatments...
November 22, 2016: Tissue Engineering. Part B, Reviews
Doaa Adel-Khattab, Francesca Giacomini, Renate Gildenhaar, Georg Berger, Cynthia Gomes, Ulf Linow, Martin Hardt, Barbara Peleska, Jens Günster, Michael Stiller, Alireza Houshmand, Khaled Abdel Ghaffar, Ahmed Gamal, Mohamed El-Mofty, Christine Knabe
Over the last decade there have been increasing efforts to develop 3D scaffolds for bone tissue engineering from bioactive ceramics with 3D printing emerging as a promising technology. The overall objective of the present study was to generate a tissue engineered synthetic bone graft with homogenously distributed osteoblasts and mineralizing bone matrix in vitro, thereby mimicking the advantageous properties of autogenous bone grafts and facilitating usage for reconstructing segmental discontinuity defects in vivo...
November 15, 2016: Journal of Tissue Engineering and Regenerative Medicine
Rafaela García-Alvarez, Isabel Izquierdo-Barba, María Vallet-Regí
Bone infection is a feared complication following surgery or trauma that remains as an extremely difficult disease to deal with. So far, the outcome of therapy could be improved with the design of 3D implants, which combine the merits of osseous regeneration and local multidrug therapy so as to avoid bacterial growth, drug resistance and the feared side effects. Herein, hierarchical 3D multidrug scaffolds based on nanocomposite bioceramic and polyvinyl alcohol (PVA) prepared by rapid prototyping with an external coating of gelatin-glutaraldehyde (Gel-Glu) have been fabricated...
November 11, 2016: Acta Biomaterialia
S Y Li, M Zhou, Y X Lai, Y M Geng, S S Cao, X M Chen
Objective: To biologically evaluate the three-dimensional(3D) printed co-poly lactic acid/glycolic acid/tri-calcium phosphate(PLGA/TCP) scaffold which could be used for repairing oral and maxillofacial bone defects, and to provide experimental evidence for its further research and clinical application. Methods: PLGA/TCP scaffolds were fabricated using low temperature rapid prototyping technique. Micro-CT and scanning electron microscope(SEM) were used to characterize the surface morphology. MC3T3-E1 cells were seeded onto the scaffold and stained with the rhodamine phalloidin and calcein acetomethoxy...
November 9, 2016: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
Wei Liu, Daming Wang, Jianghong Huang, You Wei, Jianyi Xiong, Weimin Zhu, Li Duan, Jielin Chen, Rong Sun, Daping Wang
Developed in recent years, low-temperature deposition manufacturing (LDM) represents one of the most promising rapid prototyping technologies. It is not only based on rapid deposition manufacturing process but also combined with phase separation process. Besides the controlled macropore size, tissue-engineered scaffold fabricated by LDM has inter-connected micropores in the deposited lines. More importantly, it is a green manufacturing process that involves non-heating liquefying of materials. It has been employed to fabricate tissue-engineered scaffolds for bone, cartilage, blood vessel and nerve tissue regenerations...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Tae Suk Oh, Woo Shik Jeong, Taik Jin Chang, Kyung S Koh, Jong Woo Choi
BACKGROUND: It is difficult to restore original orbital contours because of their complex 3-dimensional structure. Moreover, slight implant malpositioning can result in enophthalmos or other complications. The authors describe our experience of using individualized prebent titanium-Medpor mesh implants and stereolithographic modeling in a series of patients who underwent orbital wall reconstruction. METHODS: A consecutive series of 104 patients with orbital fractures received computer simulation-designed prebent titanium-Medpor mesh implants insertion...
October 14, 2016: Journal of Craniofacial Surgery
Mercedes Vila, Ana García, Alessandra Girotti, Matilde Alonso, Jose Carlos Rodríguez-Cabello, Arlyng González-Vázquez, Josep A Planell, Elisabeth Engel, Julia Buján, Natalio García-Honduvilla, María Vallet-Regí
: The current study reports on the manufacturing by rapid prototyping technique of three-dimensional (3D) scaffolds based on silicon substituted hydroxyapatite with Elastin-like Recombinamers (ELRs) functionalized surfaces. Silicon doped hydroxyapatite (Si-HA), with Ca10(PO4)5.7(SiO4)0.3(OH)1.7h0.3 nominal formula, was surface functionalized with two different types of polymers designed by genetic engineering: ELR-RGD that contain cell attachment specific sequences and ELR-SNA15/RGD with both hydroxyapatite and cells domains that interact with the inorganic phase and with the cells, respectively...
November 2016: Acta Biomaterialia
Jaroslav Čapek, Markéta Machová, Michaela Fousová, Jiří Kubásek, Dalibor Vojtěch, Jaroslav Fojt, Eva Jablonská, Jan Lipov, Tomáš Ruml
Recently, porous metallic materials have been extensively studied as candidates for use in the fabrication of scaffolds and augmentations to repair trabecular bone defects, e.g. in surroundings of joint replacements. Fabricating these complex structures by using common approaches (e.g., casting and machining) is very challenging. Therefore, rapid prototyping techniques, such as selective laser melting (SLM), have been investigated for these applications. In this study, we characterized a highly porous (87 vol...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Wei Xiao, Mohsen Asle Zaeem, B Sonny Bal, Mohamed N Rahaman
There is a clinical need for synthetic bioactive materials that can reliably repair intercalary skeletal tissue loss in load-bearing bones. Bioactive glasses have been investigated as one such material but their mechanical response has been a concern. Previously, we created bioactive silicate glass (13-93) scaffolds with a uniform grid-like microstructure which showed a compressive strength comparable to human cortical bone but a much lower flexural strength. In the present study, finite element modeling (FEM) was used to re-design the scaffold microstructure to improve its flexural strength without significantly lowering its compressive strength and ability to support bone infiltration in vivo...
November 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
N Gómez-Cerezo, S Sánchez-Salcedo, I Izquierdo-Barba, D Arcos, M Vallet-Regí
UNLABELLED: Mesoporous bioactive glass-polycaprolactone (MBG-PCL) scaffolds have been prepared by robocasting, a layer by layer rapid prototyping method, by stacking of individual strati. Each stratus was independently analyzed during the cell culture tests with MC3T3-E1 preosteblast-like cells. The presence of MBG stimulates the colonization of the scaffolds by increasing the cell proliferation and differentiation. MBG-PCL composites not only enhanced pre-osteoblast functions but also allowed cell movement along its surface, reaching the upper stratus faster than in pure PCL scaffolds...
October 15, 2016: Acta Biomaterialia
Heinz C Schröder, Vlad A Grebenjuk, Xiaohong Wang, Werner E G Müller
Since the first description of the silicateins, a group of enzymes that mediate the formation of the amorphous, hydrated biosilica of the skeleton of the siliceous sponges, much progress has been achieved in the understanding of this biomineralization process. These discoveries include, beside the proof of the enzymatic nature of the sponge biosilica formation, the dual property of the enzyme, to act both as a structure-forming and structure-guiding protein, and the demonstration that the initial product of silicatein is a soft, gel-like material that has to undergo a maturation process during which it achieves its favorable physical-chemical properties allowing the development of various technological or medical applications...
2016: Bioinspiration & Biomimetics
Tiago R Correia, Daniela R Figueira, Kevin D de Sá, Sónia P Miguel, Ricardo G Fradique, António G Mendonça, Ilídio J Correia
Nowadays, the incidence of bone disorders has steeply ascended and it is expected to double in the next decade, especially due to the ageing of the worldwide population. Bone defects and fractures lead to reduced patient's quality of life. Autografts, allografts and xenografts have been used to overcome different types of bone injuries, although limited availability, immune rejection or implant failure demand the development of new bone replacements. Moreover, the bacterial colonization of bone substitutes is the main cause of implant rejection...
June 3, 2016: International Journal of Biological Macromolecules
Xi Weihong, Wang Zhen, Zhu Hong-shui, Li Xiaofeng, Xiong Yuanfei
UNLABELLED: OBJECTIVE This study aims to construct a chitosan (CS)-polycaprolactone (PCL)-hydroxyapatite (HA) composite biomimetic scaffold to replace condyle and to explore the tissue engineering applications of condylar. METHODS: A resin mold of the mandibular condyle was prepared by using rapid prototyping techniques. A mandibular condylar integrated biomimetic scaffold model was prepared by solution casting-ice Lek. PCL and CS were mixed at a ratio of 4:1. HA at quality ratios of 40%, 50%, 60%, and 70% was added to groups a, b, c, and d, respectively...
February 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
Bon Kang Gu, Dong Jin Choi, Sang Jun Park, Min Sup Kim, Chang Mo Kang, Chun-Ho Kim
The 3-dimensional (3D) printing technologies, referred to as additive manufacturing (AM) or rapid prototyping (RP), have acquired reputation over the past few years for art, architectural modeling, lightweight machines, and tissue engineering applications. Among these applications, tissue engineering field using 3D printing has attracted the attention from many researchers. 3D bioprinting has an advantage in the manufacture of a scaffold for tissue engineering applications, because of rapid-fabrication, high-precision, and customized-production, etc...
2016: Biomaterials Research
Elena Canciani, Claudia Dellavia, Lorena Maria Ferreira, Chiara Giannasi, Daniela Carmagnola, Antonio Carrassi, Anna Teresa Brini
In the study, we assess a rapid prototyped scaffold composed of 30/70 hydroxyapatite (HA) and beta-tricalcium-phosphate (β-TCP) loaded with human adipose-derived stem cells (hASCs) to determine cell proliferation, differentiation toward osteogenic lineage, adhesion and penetration on/into the scaffold.In this in vitro study, hASCs isolated from fat tissue discarded after plastic surgery were expanded, characterized, and then loaded onto the scaffold. Cells were tested for: viability assay (Alamar Blue at days 3, 7 and Live/Dead at day 32), differentiation index (alkaline phosphatase activity at day 14), scaffold adhesion (standard error of the mean analysis at days 5 and 18), and penetration (ground sections at day 32)...
May 2016: Journal of Craniofacial Surgery
Annemie Houben, Jasper Van Hoorick, Jürgen Van Erps, Hugo Thienpont, Sandra Van Vlierberghe, Peter Dubruel
Over the past decades, solid freeform fabrication (SFF) has emerged as the main technology for the production of scaffolds for tissue engineering applications as a result of the architectural versatility. However, certain limitations have also arisen, primarily associated with the available, rather limited range of materials suitable for processing. To overcome these limitations, several research groups have been exploring novel methodologies through which a construct, generated via SFF, is applied as a sacrificial mould for production of the final construct...
April 14, 2016: Annals of Biomedical Engineering
Stephan Hendrikx, Christian Kascholke, Tobias Flath, Dirk Schumann, Mathias Gressenbuch, F Peter Schulze, Michael C Hacker, Michaela Schulz-Siegmund
UNLABELLED: We present a series of organic/inorganic hybrid sol-gel derived glasses, made from a tetraethoxysilane-derived silica sol (100% SiO2) and oligovalent organic crosslinkers functionalized with 3-isocyanatopropyltriethoxysilane. The material was susceptible to heat sterilization. The hybrids were processed into pore-interconnected scaffolds by an indirect rapid prototyping method, described here for the first time for sol-gel glass materials. A large panel of polyethylene oxide-derived 2- to 4-armed crosslinkers of molecular weights ranging between 170 and 8000Da were incorporated and their effect on scaffold mechanical properties was investigated...
April 15, 2016: Acta Biomaterialia
R Fradique, T R Correia, S P Miguel, K D de Sá, D R Figueira, A G Mendonça, I J Correia
The incidence of bone disorders, whether due to trauma or pathology, has been trending upward with the aging of the worldwide population. The currently available treatments for bone injuries are rather limited, involving mainly bone grafts and implants. A particularly promising approach for bone regeneration uses rapid prototyping (RP) technologies to produce 3D scaffolds with highly controlled structure and orientation, based on computer-aided design models or medical data. Herein, tricalcium phosphate (TCP)/alginate scaffolds were produced using RP and subsequently their physicochemical, mechanical and biological properties were characterized...
April 2016: Journal of Materials Science. Materials in Medicine
Sang Jin Lee, Donghyun Lee, Taek Rim Yoon, Hyung Keun Kim, Ha Hyeon Jo, Ji Sun Park, Jun Hee Lee, Wan Doo Kim, Il Keun Kwon, Su A Park
UNLABELLED: For tissue engineering, a bio-porous scaffold which is applied to bone-tissue regeneration should provide the hydrophilicity for cell attachment as well as provide for the capability to bind a bioactive molecule such as a growth factor in order to improve cell differentiation. In this work, we prepared a three-dimensional (3D) printed polycaprolactone scaffold (PCLS) grafted with recombinant human bone morphogenic protein-2 (rhBMP2) attached via polydopamine (DOPA) chemistry...
August 2016: Acta Biomaterialia
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