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https://www.readbyqxmd.com/read/29127898/capacitive-aptasensor-based-on-interdigitated-electrode-for-breast-cancer-detection-in-undiluted-human-serum
#1
Sunil K Arya, Pavel Zhurauski, Pawan Jolly, Marina R Batistuti, Marcelo Mulato, Pedro Estrela
We report the development of a simple and powerful capacitive aptasensor for the detection and estimation of human epidermal growth factor receptor 2 (HER2), a biomarker for breast cancer, in undiluted serum. The study involves the incorporation of interdigitated gold electrodes, which were used to prepare the electrochemical platform. A thiol terminated DNA aptamer with affinity for HER2 was used to prepare the bio-recognition layer via self-assembly on interdigitated gold surfaces. Non-specific binding was prevented by blocking free spaces on surface via starting block phosphate buffer saline-tween20 blocker...
November 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29126108/laboratory-scale-photobiotechnology-current-trends-and-future-perspectives
#2
Holger Morschett, Varun Loomba, Gregor Huber, Wolfgang Wiechert, Eric von Lieres, Marco Oldiges
Phototrophic bioprocesses are a promising puzzle piece in future bio-economy concepts but mostly fail for economic reasons, yet. Besides other aspects, this is mainly attributed to the omnipresent issue of optimal light supply impeding scale-up and -down of phototrophic processes according to classic established concepts. This MiniReview examines two current trends in photobiotechnology, namely microscale cultivation and modeling and simulation. Micro photobioreactors are a valuable and promising trend with microfluidic chips and microtiter plates as predominant design concepts...
November 8, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29109091/transcriptomic-profiling-of-mda-mb-231-cells-exposed-to-boswellia-serrata-and-3-o-acetyl-b-boswellic-acid-er-upr-mediated-programmed-cell-death
#3
REVIEW
Elizabeth A Mazzio, Charles A Lewis, Karam F A Soliman
BACKGROUND/AIM: Triple-negative breast cancer (TNBC) is characterized by the absence of hormone receptors (estrogen, progesterone and human epidermal growth factor receptor-2) and a relatively poor prognosis due to inefficacy of hormone receptor-based chemotherapies. It is imperative that we continue to explore natural products with potential to impede growth and metastasis of TNBC. In this study, we screened over 1,000 natural products for capacity to induce cell death in TNBC (MDA-MB -231) cells...
November 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/29083445/the-gene-diet-associations-in-postmenopausal-women-with-newly-diagnosed-dyslipidemia
#4
B Grygiel-Górniak, E Kaczmarek, M Mosor, J Przysławski, J Nowak
OBJECTIVES: The aim of this study was to determine the relationship between polymorphisms of peroxisome proliferator activated receptor - PPAR gamma-2 (Pro12Ala, C1431T) and beta 3-adrenergic receptor - ADRB3 (Trp64Arg) and dietary habits in a group of postmenopausal women who were not under hypolipidemic treatment. DESIGN: Genetic, nutritional and anthropometric parameters were measured in 213 dyslipidemic (LDL ≥115 mg/dL) and 58 normolipidemic (LDL<115) postmenopausal women...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/29083144/enhanced-cell-chip-coupling-by-rapid-femtosecond-laser-patterning-of-soft-pedot-pss-biointerfaces
#5
Francesca Santoro, Yoeri van de Burgt, Scott Tom Keene, Bianxiao Cui, Alberto Salleo
Interfacing soft materials with biological systems holds considerable promise for both biosensors and recording live cells. However, the interface between cells and organic substrates is not well studied, despite its crucial role in the effectiveness of the device. Furthermore, well-known cell adhesion enhancers, such as microgrooves, have not been implemented on these surfaces. Here, we present a nanoscale characterization of the cell-substrate interface for 3D laser-patterned organic electrodes by combining electrochemical impedance spectroscopy (EIS) and scanning electron microscopy/focused ion beam (SEM/FIB)...
November 2, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29073520/label-free-biosensors-in-the-field-of-stem-cell-biology
#6
REVIEW
Farzaneh Fathi, Reza Rahbarghazi, Mohammad-Reza Rashidi
A great body of evidence unveiled the existence of different stem cell types in various tissues with efficient capability for self-renewal and differentiation. Characterization and enumeration of a specific stem cell population seem difficult because these cells consist of a small percentage of cells within different milieu. As such, due to lack of accuracy provided by convenient methods, biosensing techniques are at the center of attention to improve stem cells characterization with high rate of sensitivity and specificity...
October 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29072035/relation-between-lifestyle-behaviors-and-body-composition-patterns-among-healthy-young-italians-a-cross-sectional-study
#7
Luciana Zaccagni, Natascia Rinaldo, Barbara Bramanti, Emanuela Gualdi-Russo
BACKGROUND: Negative lifestyle factors affect health outcomes adversely. The aim of the study was to assess body composition parameters and their relationship to several lifestyle behaviors among young Italian adults. METHODS: A cross sectional study was carried out on a sample of university students (n= 200; 50% males; 21.9 ± 2.0 years). Anthropometric and bio-impedance traits were directly measured. Behavioral data (physical activity, alcohol consumption and smoking data) were collected by a self- administered questionnaire...
October 24, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/29060300/exploration-and-validation-of-alternate-sensing-methods-for-wearable-continuous-pulse-transit-time-measurement-using-optical-and-bioimpedance-modalities
#8
Bassem Ibrahim, Viswam Nathan, Roozbeh Jafari
In this work we explore the viability of a multimodal sensing device that can be integrated in a wearable form factor for daily, non-invasive ambulatory blood pressure (BP) monitoring. A common approach in previous research has been to rely on measuring the pulse transit time (PTT), which has been shown to be correlated with the BP. In this work, we look into the feasibility of measuring PTT using sensors separated by a small distance on one arm so that any eventual realization of the system is convenient to wear and use over long periods of time...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29060187/micro-patterned-films-of-bio-functionalized-conducting-polymers-for-cellular-engineering
#9
SooHyun Park, Mohammad R Abidian, Sheereen Majd
Conducting polymers (CPs) are easy to process and have tunable physical and chemical properties including conductivity, volume, color, and hydrophobicity. Therefore, these organic polymers are attractive in a broad spectrum of bioelectronic applications ranging from implantable electrodes to biosensors and actuators. Patterned films of CPs, especially with various surface chemistries, provide versatile and sophisticated building-blocks for bioelectronics. In this context, we recently introduced a simple and efficient technique of hydrogel-mediated electropolymerization to directly pattern films of PPy (polypyrrole) with spatially-addressable chemistries...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29057827/predictors-associated-with-increase-in-skeletal-muscle-mass-after-sustained-virological-response-in-chronic-hepatitis-c-treated-with-direct-acting-antivirals
#10
Kazunori Yoh, Hiroki Nishikawa, Hirayuki Enomoto, Akio Ishii, Yoshinori Iwata, Yuho Miyamoto, Noriko Ishii, Yukihisa Yuri, Kunihiro Hasegawa, Chikage Nakano, Takashi Nishimura, Nobuhiro Aizawa, Yoshiyuki Sakai, Naoto Ikeda, Tomoyuki Takashima, Ryo Takata, Hiroko Iijima, Shuhei Nishiguchi
AIMS: We aimed to examine changes in skeletal muscle mass in chronic hepatitis C (CHC) patients undergoing interferon (IFN)-free direct acting antivirals (DAAs) therapy who achieved sustained virological response (SVR). PATIENTS AND METHODS: A total of 69 CHC patients treated with DAAs were analyzed. We compared the changes in skeletal muscle index (SMI) using bio-impedance analysis at baseline and SMI at SVR. SMI was calculated as the sum of skeletal muscle mass in upper and lower extremities divided by height squared (cm²/m²)...
October 18, 2017: Nutrients
https://www.readbyqxmd.com/read/29056754/subcutaneous-photovoltaic-infrared-energy-harvesting-for-bio-implantable-devices
#11
Eunseong Moon, David Blaauw, Jamie D Phillips
Wireless biomedical implantable devices on the mm-scale enable a wide range of applications for human health, safety, and identification, though energy harvesting and power generation are still looming challenges that impede their widespread application. Energy scavenging approaches to power biomedical implants have included thermal [1-3], kinetic [4-6], radio-frequency [7-11] and radiative sources [12-14]. However, the achievement of efficient energy scavenging for biomedical implants at the mm-scale has been elusive...
May 2017: IEEE Transactions on Electron Devices
https://www.readbyqxmd.com/read/29048152/a-new-chemometric-approach-for-automatic-identification-of-microplastics-from-environmental-compartments-based-on-ft-ir-spectroscopy
#12
Gerrit Renner, Torsten C Schmidt, Jürgen Schram
One key step studying interactions of microplastics with our ecological system is to identify plastics within environmental samples. Aging processes and surface contamination especially with biofilms impede this characterization. A complex and time-consuming cleaning procedure is a common solution for this problem. However, it implies an artificial change of sample composition with a risk of losing important information or even damaging microplastic particles. In the present work, we introduce a new chemometric approach to identify heavily weathered and contaminated microplastics without any cleaning...
November 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29034941/selective-photocatalysis-of-lignin-inspired-chemicals-by-integrating-hybrid-nanocatalysis-in-microfluidic-reactors
#13
Juan Carlos Colmenares, Rajender S Varma, Vaishakh Nair
This tutorial review assesses the available and possible strategies for attaining higher selectivity and yield of value-added chemicals from lignin using nanocatalysts embedded in a photomicroreactor. The design of the photomicroreactor, the choice of photocatalysts and the techniques for assembling the catalysts in such confined spaces are crucial elements. Lignin, a predominant industrial byproduct and pollutant, has been recognized today as a rich reservoir for aromatic chemicals and a prominent resource...
November 13, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/29028929/peripheral-i-v-analysis-piva-of-venous-waveforms-for-volume-assessment-in-patients-undergoing-haemodialysis
#14
K M Hocking, B D Alvis, F Baudenbacher, R Boyer, C M Brophy, I Beer, S Eagle
Background: The assessment of intravascular volume status remains a challenge for clinicians. Peripheral i.v. analysis (PIVA) is a method for analysing the peripheral venous waveform that has been used to monitor volume status. We present a proof-of-concept study for evaluating the efficacy of PIVA in detecting changes in fluid volume. Methods: We enrolled 37 hospitalized patients undergoing haemodialysis (HD) as a controlled model for intravascular volume loss...
September 27, 2017: British Journal of Anaesthesia
https://www.readbyqxmd.com/read/29019465/facile-and-controllable-electrochemical-fabrication-of-cell-adhesive-polypyrrole-electrodes-using-pyrrole-rgd-peptides
#15
Lindy K Jang, Semin Kim, Jiwon Seo, Jae Young Lee
Electrically conductive polymers, such as polypyrrole (PPy), have been widely used for the fabrication of various biosensors and tissue engineering scaffolds. For their biologically relevant applications, conductive biomaterials capable of intimate cellular interactions are highly desired. However, conventional methods to incorporate biomolecules into conductive polymers do not offer fine and easy control over the surface density of the biomolecules and/or their stability. We present a novel method to electrochemically immobilize cell adhesive Arg-Gly-Asp (RGD) ligands on PPy electrode surfaces with a simple control over the peptide surface density by varying the electrodeposition time...
October 11, 2017: Biofabrication
https://www.readbyqxmd.com/read/28982312/electrochemical-mip-sensors-for-drugs
#16
Aysu Yarman, Sevinc Kurbanoglu, Katharina J Jetzschmann, Sibel A Ozkan, Ulla Wollenberger, Frieder Scheller
In order to replace bio-macromolecules by stable synthetic materials in separation techniques and bioanalysis biomimetic receptors and catalysts have been developed: Functional monomers are polymerized together with the target analyte and after template removal cavities are formed in the "molecularly imprinted polymer" (MIP) which resemble the active sites of antibodies and enzymes. Staring almost 80 years ago, around 1,100 papers on MIPs were published in 2016. Electropolymerization allows to deposit MIPs directly on voltammetric electrodes or chips for quartz crystal microbalance (QCM) and surface plasmon resonance (SPR)...
October 5, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28961225/graphene-quantum-dots-electrochemistry-and-sensitive-electrocatalytic-glucose-sensor-development
#17
Sanju Gupta, Tyler Smith, Alexander Banaszak, John Boeckl
Graphene quantum dots (GQDs), derived from functionalized graphene precursors are graphene sheets a few nanometers in the lateral dimension having a several-layer thickness. They are zero-dimensional materials with quantum confinement and edge site effects. Intense research interest in GQDs is attributed to their unique physicochemical phenomena arising from the sp²-bonded carbon nanocore surrounded with edged plane functional moieties. In this work, GQDs are synthesized by both solvothermal and hydrothermal techniques, with the optimal size of 5 nm determined using high-resolution transmission electron microscopy, with additional UV-Vis absorption and fluorescence spectroscopy, revealing electronic band signatures in the blue-violet region...
September 29, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28960957/in-vivo-neuroimaging-of-exosomes-using-gold-nanoparticles
#18
Oshra Betzer, Nisim Perets, Ariel Angel, Menachem Motiei, Tamar Sadan, Gal Yadid, Daniel Offen, Rachela Popovtzer
Exosomes are emerging as effective therapeutic tools for various pathologies. These extracellular vesicles can bypass biological barriers, including the blood-brain barrier, and can serve as powerful drug and gene therapy transporters. However, the progress of therapy development is impeded by several challenges, including insufficient data on exosome trafficking and biodistribution and the difficulty to image deep brain structures in vivo. Herein, we established a method for noninvasive in vivo neuroimaging and tracking of exosomes, based on glucose-coated gold nanoparticle (GNP) labeling and computed tomography imaging...
October 5, 2017: ACS Nano
https://www.readbyqxmd.com/read/28960919/exploring-the-reaction-pathways-of-bio-glycerol-hydro-deoxygenation-to-propene-over-molybdena-based-catalysts
#19
Vasiliki Zacharopoulou, Efterpi Vasiliadou, Angeliki A Lemonidou
One-step glycerol reaction with hydrogen, selectively forming propene, is a novel and particularly challenging catalytic pathway that has not yet been thoroughly explored in the open literature. Molybdena-based catalysts are active and selective to C-O bond scission; propene is the only product in the gas phase, under the standard reaction conditions, impeding further hydrogenation to propane. Within this context, this work focuses on exploration of the reaction pathways and the investigation of various parameters, affecting the catalytic performance, such as the role of hydrogen on the product distribution and the effect of the catalyst pretreatment step...
September 28, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28952454/reference-centile-curves-for-body-fat-percentage-fat-free-mass-muscle-mass-and-bone-mass-measured-by-bioelectrical-impedance-in-asian-indian-children-and-adolescents
#20
Shashi Chiplonkar, Neha Kajale, Veena Ekbote, Rubina Mandlik, Lavanya Parthasarathy, Ashwin Borade, Pinal Patel, Prerna Patel, Vaman Khadilkar, Anuradha Khadilkar
OBJECTIVE: To create gender-specific percentile curves for percent body fat (%BF) by Bio electrical Impedance Analysis ( BIA) for screening adiposity and risk of hypertension in Indian children and generate reference curves for percent fat-free mass (%FFM), muscle mass (%LM) and bone mineral content (BMC) by using bioelectrical impedance. DESIGN: Multicenter cross-sectional study. SETTING: Private schools from major Indian cities from 5 regions of India...
September 26, 2017: Indian Pediatrics
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