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https://www.readbyqxmd.com/read/28324970/development-of-data-acquisition-components-for-simultaneous-recording-of-3d-epicardial-and-surface-ecg-signals-in-the-langendorff-perfusion-apparatus
#1
Ryan Wade Selby, Anup Jonchhe, Chen Kaplan, Coeli M Lopes, Behnaz Ghoraani
Sudden Cardiac Death (SCD) claims 7 million lives per year. The importance of myocardial electrogram (EGM) repolarization alternans and surface electrocardiogram (ECG) T-wave alternans is gaining traction for understanding the underlying SCD mechanisms. However, the relationship between the 3D spatial distribution of myocardial EGMs and surface ECG with respect to SCD has yet to be investigated. To make this happen, a modified data acquisition system has been developed and fabricated in conjunction with the Langendorff perfusion system to enable simultaneous recording and analysis of the 3D spatial distribution of myocardial EGMs and the surface ECG...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28296232/inhibitors-of-soluble-epoxide-hydrolase-minimize-ischemia-reperfusion-induced-cardiac-damage-in-normal-hypertensive-and-diabetic-rats
#2
Oliul Islam, Prashanth Patil, Sumanta K Goswami, Rema Razdan, Mohammed N Inamdar, Mohammed Rizwan, Jubin Mathew, Bora Inceoglu, Kin S Stephen Lee, Sung H Hwang, Bruce D Hammock
AIM: We designed a study to evaluate the cardioprotective effect of two soluble epoxide hydrolase (sEH) inhibitors, 1-(1-propanoylpiperidin-4-yl)-3-(4-trifluoromethoxy)phenyl)urea (TPPU) and trans-4-{4-[3-(4-trifluoromethoxyphenyl)-ureido]cyclohexyloxy}benzoic acid (t-TUCB), in ischemia-reperfusion (IR) model. METHODS: Cardioprotective effects of the sEH inhibitors were evaluated against IR-induced myocardial damage in hearts from normal, hypertensive and diabetic rats using Langendorff's apparatus...
March 15, 2017: Cardiovascular Therapeutics
https://www.readbyqxmd.com/read/28290047/adaptation-to-chronic-continuous-hypoxia-potentiates-akt-hk2-anti-apoptotic-pathway-during-brief-myocardial-ischemia-reperfusion-insult
#3
David Kolar, Milada Gresikova, Petra Waskova-Arnostova, Barbara Elsnicova, Jana Kohutova, Daniela Hornikova, Pavel Vebr, Jan Neckar, Tereza Blahova, Dita Kasparova, Jiri Novotny, Frantisek Kolar, Olga Novakova, Jitka M Zurmanova
Adaptation to chronic hypoxia represents a potential cardioprotective intervention reducing the extent of acute ischemia/reperfusion (I/R) injury, which is a major cause of death worldwide. The main objective of this study was to investigate the anti-apoptotic Akt/hexokinase 2 (HK2) pathway in hypoxic hearts subjected to I/R insult. Hearts isolated from male Wistar rats exposed either to continuous normobaric hypoxia (CNH; 10% O2) or to room air for 3 weeks were perfused according to Langendorff and subjected to 10 min of no-flow ischemia and 10 min of reperfusion...
March 13, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28282374/depletion-of-cardiac-catecholamine-stores-impairs-cardiac-norepinephrine-re-uptake-by-downregulation-of-the-norepinephrine-transporter
#4
Michael M Kreusser, Lorenz H Lehmann, Markus Haass, Sebastian J Buss, Hugo A Katus, Dirk Lossnitzer
In heart failure (HF), a disturbed cardiac norepinephrine (NE) homeostasis is characterized by depleted cardiac NE stores, impairment of the cardiac NE re-uptake by the neuronal norepinephrine transporter (NET) and enhanced cardiac NE net release. Reduced cardiac NE content appears to be caused by enhanced cardiac NE net release from sympathetic neurons in HF, triggered by neurohumoral activation. However, it remains unclear whether reduced NE itself has an impact on cardiac NE re-uptake, independent of neurohumoral activation...
2017: PloS One
https://www.readbyqxmd.com/read/28278587/functionality-of-albumin-derived-perfluorocarbon-based-artificial-oxygen-carriers-in-the-langendorff-heart-%C3%A2
#5
Anna Wrobeln, Klaus D Schlüter, Jürgen Linders, Manfred Zähres, Christian Mayer, Michael Kirsch, Katja B Ferenz
The aim of this study was to prove whether albumin-derived perfluorocarbon-based nanoparticles (capsules) can operate as a novel artificial oxygen carrier in a rat Langendorff-heart perfusion model. Hearts perfused with capsules showed increased left ventricular pressure and rate pressure product compared to hearts perfused with pure Krebs-Henseleit (KH)-buffer. The capsules prevented the myocardium from functional fail when in their absence a noxious ischemia was observed. Capsules did not change rheological properties of KH-buffer and could repeatedly reload with oxygen...
February 8, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28276718/insulin-and-gsk3%C3%AE-inhibition-abrogates-the-infarct-sparing-effect-of-ischemic-postconditioning-in-ex-vivo-rat-hearts
#6
Erik Helgeland, Anita Wergeland, Rune M Sandøy, Maren Askeland, Anne Aspevik, Lars Breivik, Anne K Jonassen
OBJECTIVES: Pharmacological treatment of reperfusion injury using insulin and GSK3β inhibition has been shown to be cardioprotective, however, their interaction with the endogenous cardioprotective strategy, ischemic postconditioning, is not known. DESIGN: Langendorff perfused ex vivo rat hearts were subjected to 30 min of regional ischemia and 120 min of reperfusion. For the first 15 min of reperfusion hearts received either vehicle (Ctr), insulin (Ins) or a GSK3β inhibitor (SB415286; SB41), with or without interruption of ischemic postconditioning (IPost; 3 × 30 s of global ischemia)...
February 13, 2017: Scandinavian Cardiovascular Journal: SCJ
https://www.readbyqxmd.com/read/28258543/reducing-mitochondrial-bound-hexokinase-ii-mediates-transition-from-non-injurious-into-injurious-ischemia-reperfusion-of-the-intact-heart
#7
Rianne Nederlof, Ebru Gürel-Gurevin, Otto Eerbeek, Chaoqin Xie, G Sjoerd Deijs, Moritz Konkel, Jun Hu, Nina C Weber, Cees A Schumacher, Antonius Baartscheer, Egbert G Mik, Markus W Hollmann, Fadi G Akar, Coert J Zuurbier
Ischemia/reperfusion (I/R) of the heart becomes injurious when duration of the ischemic insult exceeds a certain threshold (approximately ≥20 min). Mitochondrial bound hexokinase II (mtHKII) protects against I/R injury, with the amount of mtHKII correlating with injury. Here, we examine whether mtHKII can induce the transition from non-injurious to injurious I/R, by detaching HKII from mitochondria during a non-injurious I/R interval. Additionally, we examine possible underlying mechanisms (increased reactive oxygen species (ROS), increased oxygen consumption (MVO2) and decreased cardiac energetics) associated with this transition...
March 3, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28244203/decrease-in-long-chain-acylcarnitine-tissue-content-determines-the-duration-of-and-correlates-with-the-cardioprotective-effect-of-methyl-gbb
#8
Janis Kuka, Marina Makrecka-Kuka, Helena Cirule, Solveiga Grinberga, Eduards Sevostjanovs, Maija Dambrova, Edgars Liepinsh
Ischaemia in the heart is accompanied by the accumulation of long-chain acylcarnitines (LCACs) which is one of the multiple factors that contribute to the ischaemia-reperfusion damage development. Long-term pre-treatment that decreases carnitine and LCAC contents also reduces ischaemia-reperfusion (IR) damage; however, the duration of the post-treatment effects is not known. The aim of the study was to assess the post-treatment effects of the carnitine transport (OCTN2) inhibitor, methyl-GBB, on LCAC content and the duration of its cardioprotective effect...
February 28, 2017: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28229626/the-role-of-hydrogen-sulfide-in-homocysteine-induced-cardiodynamic-effects-and-oxidative-stress-markers-in-the-isolated-rat-heart
#9
M Stojanovic, V Zivkovic, I Srejovic, V Jakovljevic, N Jeremic, D Djuric
This study aimed to assess the role of H2S in homocysteine-induced cardiodynamic effects in the isolated rat heart. The hearts were retrogradely perfused according to the Langendorff technique. The maximum and minimum rates of pressure in the left ventricle (dp/dt max, dp/dt min), systolic and diastolic left ventricular pressures (SLVP, DLVP), heart rate (HR), and coronary flow (CF) were measured. A spectrophotometrical method was used to measure the following oxidative stress markers: index of lipid peroxidation (thiobarbituric acid reactive substances, TBARS), nitrite level (NO2(-)), superoxide anion radicals (O2(•-)), and hydrogen peroxide (H2O2) concentrations...
December 2016: Physiol Int
https://www.readbyqxmd.com/read/28227084/development-of-data-acquisition-components-for-simultaneous-recording-of-3d-epicardial-and-surface-ecg-signals-in-the-langendorff-perfusion-apparatus
#10
Ryan Wade Selby, Anup Jonchhe, Chen Kaplan, Coeli M Lopes, Behnaz Ghoraani, Ryan Wade Selby, Anup Jonchhe, Chen Kaplan, Coeli M Lopes, Behnaz Ghoraani, Chen Kaplan, Behnaz Ghoraani, Anup Jonchhe, Coeli M Lopes, Ryan Wade Selby
Sudden Cardiac Death (SCD) claims 7 million lives per year. The importance of myocardial electrogram (EGM) repolarization alternans and surface electrocardiogram (ECG) T-wave alternans is gaining traction for understanding the underlying SCD mechanisms. However, the relationship between the 3D spatial distribution of myocardial EGMs and surface ECG with respect to SCD has yet to be investigated. To make this happen, a modified data acquisition system has been developed and fabricated in conjunction with the Langendorff perfusion system to enable simultaneous recording and analysis of the 3D spatial distribution of myocardial EGMs and the surface ECG...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28219356/bacopa-monnieri-extract-increases-rat-coronary-flow-and-protects-against-myocardial-ischemia-reperfusion-injury
#11
Sirintorn Srimachai, Sylvie Devaux, Celine Demougeot, Sarawut Kumphune, Nina D Ullrich, Ernst Niggli, Kornkanok Ingkaninan, Natakorn Kamkaew, C Norman Scholfield, Sompol Tapechum, Krongkarn Chootip
BACKGROUND: This study explored Bacopa monnieri, a medicinal Ayurvedic herb, as a cardioprotectant against ischemia/reperfusion injury using cardiac function and coronary flow as end-points. METHODS: In normal isolated rat hearts, coronary flow, left ventricular developed pressure, heart rate, and functional recovery were measured using the Langendorff preparation. Hearts were perfused with either (i) Krebs-Henseleit (normal) solution, (control), or with 30, 100 μg/ml B...
February 20, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28214609/evaluation-of-vascular-tone-and-cardiac-contractility-in-response-to-silver-nanoparticles-using-langendorff-rat-heart-preparation
#12
Ramirez-Lee Manuel Alejandro, Martinez-Cuevas Pedro Pablo, Rosas-Hernandez Hector, Oros-Ovalle Cuauhtémoc, Bravo-Sanchez Mariela, Martinez-Castañon Gabriel Alejandro, Gonzalez Carmen
Silver nanoparticles (AgNPs) have been widely used because of their antimicrobial properties. However, several reports suggest that AgNPs exposure promote cardiac effects that involve nitric oxide (NO) and oxidative stress (OS). Nevertheless, there are no studies related to AgNPs-induced effects in cardiac physiology. The aim of this study was to evaluate the AgNPs direct actions on coronary vascular tone and cardiac contractility using Langendorff rat heart preparation. Low concentrations of AgNPs (0.1 and 1 μg/mL) increased NO derived from inducible NO-synthase (iNOS), without modifying cardiac parameters...
February 16, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28213770/myosin-heavy-chain-and-cardiac-troponin-t-damage-is-associated-with-impaired-myofibrillar-atpase-activity-contributing-to-sarcomeric-dysfunction-in-ca-2-paradox-rat-hearts
#13
Árpád Kovács, Judit Kalász, Enikő T Pásztor, Attila Tóth, Zoltán Papp, Naranjan S Dhalla, Judit Barta
This study aimed to explore the potential contribution of myofibrils to contractile dysfunction in Ca(2+)-paradox hearts. Isolated rat hearts were perfused with Krebs-Henseleit solution (Control), followed by Ca(2+)-depletion, and then Ca(2+)-repletion after Ca(2+)-depletion (Ca(2+)-paradox) by Langendorff method. During heart perfusion left ventricular developed pressure (LVDP), end-diastolic pressure (LVEDP), rate of pressure development (+ dP/dt), and pressure decay (-dP/dt) were registered. Control LVDP (127...
February 17, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28202059/cardiosphere-derived-cells-do-not-improve-cardiac-function-in-rats-with-cardiac-failure
#14
Taís Hanae Kasai-Brunswick, Andréa Rodrigues da Costa, Raiana Andrade Quintanilha Barbosa, Bruna Farjun, Fernanda Cristina Paccola Mesquita, Danúbia Silva Dos Santos, Isalira Peroba Ramos, Grazielle Suhett, Guilherme Visconde Brasil, Sandro Torrentes da Cunha, José Oscar R Brito, Juliana do Amaral Passipieri, Adriana Bastos Carvalho, Antonio Carlos Campos de Carvalho
BACKGROUND: Heart failure represents an important public health issue due to its high costs and growing incidence worldwide. Evidence showing the regenerative potential of postmitotic heart tissue has suggested the existence of endogenous cardiac stem cells in adult hearts. Cardiosphere-derived cells (CDC) constitute a candidate pool of such cardiac stem cells. Previous studies using acute myocardial infarction (MI) models in rodents demonstrated an improvement in cardiac function after cell therapy with CDC...
February 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28195947/alpha-msh-induces-vasodilatation-and-exerts-cardioprotection-via-the-heme-oxygenase-pathway-in-rat-hearts
#15
Miklos Vecsernyes, Miklos Szokol, Mariann Bombicz, Daniel Priksz, Rudolf Gesztelyi, Gabor Aron Fulop, Balazs Varga, Bela Juhasz, David Haines, Arpad Tosaki
Alpha-melanocyte-stimulating hormone (α-MSH) is a protein with known capacity for protection against cardiovascular ischemia-reperfusion (I/R) injury. The present investigation evaluates the capacity of α-MSH to mitigate I/R effects in an isolated working rat heart model and determine the dependency of these alterations on the activity of heme oxygenase-1 (HO-1, hsp-32), a heat shock protein that functions as a major antioxidant defense molecule. Healthy male Sprague-Dawley rats were used for all experiments...
February 13, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28194639/ultrafine-particulate-matter-increases-cardiac-ischemia-reperfusion-injury-via-mitochondrial-permeability-transition-pore
#16
Nathan A Holland, Chad R Fraiser, Ruben C Sloan, Robert B Devlin, David A Brown, Christopher J Wingard
Ultrafine particulate matter (UFP) has been associated with increased cardiovascular morbidity and mortality. However, the mechanisms that drive PM-associated cardiovascular disease and dysfunction remain unclear. We examined the impact of oropharyngeal aspiration of 100 μg UFP from the Chapel Hill, NC, air shed in Sprague-Dawley rats on cardiac function, arrhythmogenesis, and cardiac ischemia/reperfusion (I/R) injury using a Langendorff working heart model. We found that exposure to UFP was capable of significantly exacerbating cardiac I/R injury without changing overall cardiac function or major changes in arrhythmogenesis...
February 13, 2017: Cardiovascular Toxicology
https://www.readbyqxmd.com/read/28194544/exploring-the-role-of-trpv-and-cgrp-in-adenosine-preconditioning-and-remote-hind-limb-preconditioning-induced-cardioprotection-in-rats
#17
Amritpal Singh, Puneet Kaur Randhawa, Anjana Bali, Nirmal Singh, Amteshwar Singh Jaggi
The cardioprotective effects of remote hind limb preconditioning (RIPC) are well known, but mechanisms by which protection occurs still remain to be explored. Therefore, the present study was designed to investigate the role of TRPV and CGRP in adenosine and remote preconditioning-induced cardioprotection, using sumatriptan, a CGRP release inhibitor and ruthenium red, a TRPV inhibitor, in rats. For remote preconditioning, a pressure cuff was tied around the hind limb of the rat and was inflated with air up to 150 mmHg to produce ischemia in the hind limb and during reperfusion pressure was released...
February 14, 2017: Cardiovascular Drugs and Therapy
https://www.readbyqxmd.com/read/28187689/evaluation-of-antihypertensive-potential-of-ficus-carica-fruit
#18
Alamgeer, Shifa Iman, Hira Asif, Muhammad Saleem
CONTEXT: Ficus carica L. (Moraceae) fruit is said to possess cardiovascular activity and has been used empirically in traditional phytotherapies for the treatment of hypertension and various other cardiovascular diseases. OBJECTIVE: This study investigated the antihypertensive and cardioinhibitory activity of the aqueous-methanol extract of F. carica fruit in rats. MATERIALS AND METHODS: Extract in 250, 500 and 1000 mg/kg doses (p.o.) were administered to normotensive Sprague Dawley rats and blood pressure was measured using non-invasive technique...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28186163/label-free-detection-of-myocardial-ischaemia-in-the-perfused-rat-heart-by-spontaneous-raman-spectroscopy
#19
Suguru Ohira, Hideo Tanaka, Yoshinori Harada, Takeo Minamikawa, Yasuaki Kumamoto, Satoaki Matoba, Hitoshi Yaku, Tetsuro Takamatsu
Raman spectroscopy, which identifies intrinsic molecular constituents, has a potential for determining myocardial viability under label-free conditions. However, its suitability for evaluating myocardial ischaemia is undetermined. Focusing on cytochromes, i.e., representative molecules reflecting mitochondrial activity, we tested whether Raman spectroscopy is applicable for evaluating myocardial ischaemia especially during early ischaemic phase. We obtained spontaneous Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart upon 532-nm excitation before and during the "stopped-flow," global ischaemia...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28179209/administration-of-anabolic-steroid-during-adolescence-induces-long-term-cardiac-hypertrophy-and-increases-susceptibility-to-ischemia-reperfusion-injury-in-adult-wistar-rats
#20
Fernando de Azevedo Cruz Seara, Raiana Andrade Quintanilha Barbosa, Dahienne Ferreira de Oliveira, Diorney Luiz Souza Gran da Silva, Adriana Bastos Carvalho, Andrea Claudia Freitas Ferreira, José Hamilton Matheus Nascimento, Emerson Lopes Olivares
Chronic administration of anabolic androgenic steroids (AAS) in adult rats results in cardiac hypertrophy and increased susceptibility to myocardial ischemia/reperfusion (IR) injury. Molecular analyses demonstrated that hyperactivation of type 1 angiotensin II (AT1) receptor mediates cardiac hypertrophy induced by AAS and also induces down-regulation of myocardial ATP-sensitive potassium channel (KATP), resulting in loss of exercise-induced cardioprotection. Exposure to AAS during adolescence promoted long-term cardiovascular dysfunctions, such as dysautonomia...
February 5, 2017: Journal of Steroid Biochemistry and Molecular Biology
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