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langendorff rat heart

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https://www.readbyqxmd.com/read/28214609/evaluation-of-vascular-tone-and-cardiac-contractility-in-response-to-silver-nanoparticles-using-langendorff-rat-heart-preparation
#1
Ramirez-Lee Manuel Alejandro, Martinez-Cuevas Pedro Pablo, Rosas-Hernandez Hector, Oros-Ovalle Cuauhtémoc, Bravo-Sanchez Mariela, Martinez-Castañon Gabriel Alejandro, Gonzalez Carmen
Silver nanoparticles (AgNPs) have been widely used because of their antimicrobial properties. However, several reports suggest that AgNPs exposure promote cardiac effects that involve nitric oxide (NO) and oxidative stress (OS). Nevertheless, there are no studies related to AgNPs-induced effects in cardiac physiology. The aim of this study was to evaluate the AgNPs direct actions on coronary vascular tone and cardiac contractility using Langendorff rat heart preparation. Low concentrations of AgNPs (0.1 and 1μg/mL) increased NO derived from inducible NO-synthase (iNOS), without modifying cardiac parameters...
February 15, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28213770/myosin-heavy-chain-and-cardiac-troponin-t-damage-is-associated-with-impaired-myofibrillar-atpase-activity-contributing-to-sarcomeric-dysfunction-in-ca-2-paradox-rat-hearts
#2
Árpád Kovács, Judit Kalász, Enikő T Pásztor, Attila Tóth, Zoltán Papp, Naranjan S Dhalla, Judit Barta
This study aimed to explore the potential contribution of myofibrils to contractile dysfunction in Ca(2+)-paradox hearts. Isolated rat hearts were perfused with Krebs-Henseleit solution (Control), followed by Ca(2+)-depletion, and then Ca(2+)-repletion after Ca(2+)-depletion (Ca(2+)-paradox) by Langendorff method. During heart perfusion left ventricular developed pressure (LVDP), end-diastolic pressure (LVEDP), rate of pressure development (+ dP/dt), and pressure decay (-dP/dt) were registered. Control LVDP (127...
February 17, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28202059/cardiosphere-derived-cells-do-not-improve-cardiac-function-in-rats-with-cardiac-failure
#3
Taís Hanae Kasai-Brunswick, Andréa Rodrigues da Costa, Raiana Andrade Quintanilha Barbosa, Bruna Farjun, Fernanda Cristina Paccola Mesquita, Danúbia Silva Dos Santos, Isalira Peroba Ramos, Grazielle Suhett, Guilherme Visconde Brasil, Sandro Torrentes da Cunha, José Oscar R Brito, Juliana do Amaral Passipieri, Adriana Bastos Carvalho, Antonio Carlos Campos de Carvalho
BACKGROUND: Heart failure represents an important public health issue due to its high costs and growing incidence worldwide. Evidence showing the regenerative potential of postmitotic heart tissue has suggested the existence of endogenous cardiac stem cells in adult hearts. Cardiosphere-derived cells (CDC) constitute a candidate pool of such cardiac stem cells. Previous studies using acute myocardial infarction (MI) models in rodents demonstrated an improvement in cardiac function after cell therapy with CDC...
February 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28195947/alpha-msh-induces-vasodilatation-and-exerts-cardioprotection-via-the-heme-oxygenase-pathway-in-rat-hearts
#4
Miklos Vecsernyes, Miklos Szokol, Mariann Bombicz, Daniel Priksz, Rudolf Gesztelyi, Gabor Aron Fulop, Balazs Varga, Bela Juhasz, David Haines, Arpad Tosaki
Alpha-melanocyte-stimulating hormone (α-MSH) is a protein with known capacity for protection against cardiovascular ischemia-reperfusion (I/R) injury. The present investigation evaluates the capacity of α-MSH to mitigate I/R effects in an isolated working rat heart model and determine the dependency of these alterations on the activity of heme oxygenase-1 (HO-1, hsp-32), a heat shock protein that functions as a major antioxidant defense molecule. Healthy male Sprague-Dawley rats were used for all experiments...
February 13, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28194639/ultrafine-particulate-matter-increases-cardiac-ischemia-reperfusion-injury-via-mitochondrial-permeability-transition-pore
#5
Nathan A Holland, Chad R Fraiser, Ruben C Sloan, Robert B Devlin, David A Brown, Christopher J Wingard
Ultrafine particulate matter (UFP) has been associated with increased cardiovascular morbidity and mortality. However, the mechanisms that drive PM-associated cardiovascular disease and dysfunction remain unclear. We examined the impact of oropharyngeal aspiration of 100 μg UFP from the Chapel Hill, NC, air shed in Sprague-Dawley rats on cardiac function, arrhythmogenesis, and cardiac ischemia/reperfusion (I/R) injury using a Langendorff working heart model. We found that exposure to UFP was capable of significantly exacerbating cardiac I/R injury without changing overall cardiac function or major changes in arrhythmogenesis...
February 13, 2017: Cardiovascular Toxicology
https://www.readbyqxmd.com/read/28194544/exploring-the-role-of-trpv-and-cgrp-in-adenosine-preconditioning-and-remote-hind-limb-preconditioning-induced-cardioprotection-in-rats
#6
Amritpal Singh, Puneet Kaur Randhawa, Anjana Bali, Nirmal Singh, Amteshwar Singh Jaggi
The cardioprotective effects of remote hind limb preconditioning (RIPC) are well known, but mechanisms by which protection occurs still remain to be explored. Therefore, the present study was designed to investigate the role of TRPV and CGRP in adenosine and remote preconditioning-induced cardioprotection, using sumatriptan, a CGRP release inhibitor and ruthenium red, a TRPV inhibitor, in rats. For remote preconditioning, a pressure cuff was tied around the hind limb of the rat and was inflated with air up to 150 mmHg to produce ischemia in the hind limb and during reperfusion pressure was released...
February 14, 2017: Cardiovascular Drugs and Therapy
https://www.readbyqxmd.com/read/28187689/evaluation-of-antihypertensive-potential-of-ficus-carica-fruit
#7
Alamgeer, Shifa Iman, Hira Asif, Muhammad Saleem
CONTEXT: Ficus carica L. (Moraceae) fruit is said to possess cardiovascular activity and has been used empirically in traditional phytotherapies for the treatment of hypertension and various other cardiovascular diseases. OBJECTIVE: This study investigated the antihypertensive and cardioinhibitory activity of the aqueous-methanol extract of F. carica fruit in rats. MATERIALS AND METHODS: Extract in 250, 500 and 1000 mg/kg doses (p.o.) were administered to normotensive Sprague Dawley rats and blood pressure was measured using non-invasive technique...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28186163/label-free-detection-of-myocardial-ischaemia-in-the-perfused-rat-heart-by-spontaneous-raman-spectroscopy
#8
Suguru Ohira, Hideo Tanaka, Yoshinori Harada, Takeo Minamikawa, Yasuaki Kumamoto, Satoaki Matoba, Hitoshi Yaku, Tetsuro Takamatsu
Raman spectroscopy, which identifies intrinsic molecular constituents, has a potential for determining myocardial viability under label-free conditions. However, its suitability for evaluating myocardial ischaemia is undetermined. Focusing on cytochromes, i.e., representative molecules reflecting mitochondrial activity, we tested whether Raman spectroscopy is applicable for evaluating myocardial ischaemia especially during early ischaemic phase. We obtained spontaneous Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart upon 532-nm excitation before and during the "stopped-flow," global ischaemia...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28179209/administration-of-anabolic-steroid-during-adolescence-induces-long-term-cardiac-hypertrophy-and-increases-susceptibility-to-ischemia-reperfusion-injury-in-adult-wistar-rats
#9
Fernando de Azevedo Cruz Seara, Raiana Andrade Quintanilha Barbosa, Dahienne Ferreira de Oliveira, Diorney Luiz Souza Gran da Silva, Adriana Bastos Carvalho, Andrea Claudia Freitas Ferreira, José Hamilton Matheus Nascimento, Emerson Lopes Olivares
Chronic administration of anabolic androgenic steroids (AAS) in adult rats results in cardiac hypertrophy and increased susceptibility to myocardial ischemia/reperfusion (IR) injury. Molecular analyses demonstrated that hyperactivation of type 1 angiotensin II (AT1) receptor mediates cardiac hypertrophy induced by AAS and also induces down-regulation of myocardial ATP-sensitive potassium channel (KATP), resulting in loss of exercise-induced cardioprotection. Exposure to AAS during adolescence promoted long-term cardiovascular dysfunctions, such as dysautonomia...
February 5, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28177679/reduced-expression-of-hcn-channels-in-sinoatrial-node-of-streptozotocin-induced-diabetic-rats
#10
Xin Huang, Nier Zhong, Hong Zhang, Aiqun Ma, Zuyi Yuan, Ning Guo
Diabetes mellitus (DM) is associated with an electrical remodeling of the heart, increasing risk of arrhythmias. However, knowledge of electrical remodeling in sinoatrial node (SAN) by DM is limited. We investigated the expression of HCN channel isoforms, HCN1-HCN4, in SAN from streptozotocin (STZ)-induced diabetic rats and the age-matched controls. We found that the STZ-induced diabetic rats have a lower intrinsic heart rate, a lengthened sinoatrial conduction time and rate-corrected maximal sinoatrial node recovery time in vivo as well as a longer cycle length (CL) in vitro as compared with the control...
December 23, 2016: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28162790/myocardial-ischemia-reperfusion-upregulates-the-transcription-of-the-neuregulin1-receptor-erbb3-but-only-postconditioning-preserves-protein-translation-role-in-oxidative-stress
#11
Michela Morano, Carmelina Angotti, Francesca Tullio, Giovanna Gambarotta, Claudia Penna, Pasquale Pagliaro, Stefano Geuna
BACKGROUND: Neuregulin1 (Nrg1) and its receptors ErbB are crucial for heart development and for adult heart structural maintenance and function and Nrg1 has been proposed for heart failure treatment. Infarct size is the major determinant of heart failure and the mechanism of action and the role of each ErbB receptor remain obscure, especially in the post-ischemic myocardium. We hypothesized that Nrg1 and ErbB are affected at transcriptional level early after ischemia/reperfusion (I/R) injury, and that the protective postconditioning procedure (PostC, brief cycles of ischemia/reperfusion carried out after a sustained ischemia) can influence this pathway...
January 31, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28091727/intrinsic-cardiac-ganglia-and-acetylcholine-are-important-in-the-mechanism-of-ischaemic-preconditioning
#12
J M J Pickard, N Burke, S M Davidson, D M Yellon
This study aimed to investigate the role of the intrinsic cardiac nervous system in the mechanism of classical myocardial ischaemic preconditioning (IPC). Isolated perfused rat hearts were subjected to 35-min regional ischaemia and 60-min reperfusion. IPC was induced as three cycles of 5-min global ischaemia-reperfusion, and provided significant reduction in infarct size (IS/AAR = 14 ± 2% vs control IS/AAR = 48 ± 3%, p < 0.05). Treatment with the ganglionic antagonist, hexamethonium (50 μM), blocked IPC protection (IS/AAR = 37 ± 7%, p < 0...
March 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28087358/vascular-calcification-abrogates-the-nicorandil-mediated-cardio-protection-in-ischemia-reperfusion-injury-of-rat-heart
#13
Sriram Ravindran, Jeyashri Murali, Sunil Kumar Amirthalingam, Senthilkumar Gopalakrishnan, Gino A Kurian
The present study was aimed to determine the efficacy of nicorandil in treating cardiac reperfusion injury with an underlying co-morbidity of vascular calcification (VC). Adenine diet was used to induce VC in Wistar rat and the heart was isolated to induce global ischemia reperfusion (IR) by Langendorff method, with and without the nicorandil (7.5mg/kg) pre-treatment and compared with those fed on normal diet. The adenine-treated rats displayed abnormal ECG changes and altered mitochondrial integrity compared to a normal rat heart...
January 10, 2017: Vascular Pharmacology
https://www.readbyqxmd.com/read/28081514/role-of-mirna-1-in-regulating-connexin-43-in-ischemia-reperfusion-heart-injury-a-rat-model
#14
Bo Bian, Xue-Fang Yu, Guo-Qin Wang, Tian-Ming Teng
MiRNA-1 may participate in regulating ischemia-reperfusion injury (IRI) by affecting the expression and distribution of connexin 43 (Cx43). The aim of this study is to investigate miR-1 expression and its potential role in regulating Cx43 during ischemic postconditioning (IPOST) in a rat model. Fifty-five Wistar male rats were randomly divided into five groups: N, IR, IPOST, agomir-1, and antagomir-1 group. The hearts were perfused with the Langendorff system. The reperfusion arrhythmia (RA) and myocardial infarct size were observed and recorded...
January 3, 2017: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/28079401/hemo2life-as-a-protective-additive-to-celsior-solution-for-static-storage-of-donor-hearts-prior-to-transplantation
#15
Elaine S Teh, Franck Zal, Valérie Polard, Philippe Menasché, David J Chambers
BACKGROUND: Prior to heart transplantation, static storage of donor hearts is currently limited to 4-5 h, despite profound hypothermia (4-8 °C). Because heart transplantation is an emergency procedure, improved protection to extend safe storage duration would be advantageous. We investigated whether the naturally respiratory pigment HEMO2life(®), which is effective at hypothermia for the passive release of oxygen via oxygen gradient, could improve long-term preservation. METHODS: Isolated Langendorff-perfused rat hearts (n = 12/group) were equilibrated (20 min) and function (left ventricular developed pressure: LVDP) measured by intraventricular balloon before arrest with cold (7...
January 12, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28071786/functional-and-cardioprotective-effects-of-simultaneous-and-individual-activation-of-protein-kinase-a-and-epac
#16
Igor Khaliulin, Mark Bond, Andrew F James, Zara Dyar, Raheleh Amini, Jason L Johnson, M-Saadeh Suleiman
BACKGROUND AND PURPOSE: Myocardial cAMP elevation confers cardioprotection against ischaemia/reperfusion (I/R) injury. cAMP activates two independent signalling pathways, PKA and Epac. This study investigated the cardiac effects of activating PKA and/or Epac and their involvement in cardioprotection against I/R. EXPERIMENTAL APPROACH: Hearts extracted from male rats were used either for determination of PKA and PKC activation or perfused in the Langendorff mode for either cardiomyocytes isolation or used to monitor functional activity at basal levels and after 30 min global ischaemia and 2 h reperfusion...
January 10, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28057852/endothelin-1-and-et-receptors-impair-left-ventricular-function-by-mediated-coronary-arteries-dysfunction-in-chronic-intermittent-hypoxia-rats
#17
Jin-Wei Wang, Ai-Ying Li, Qiu-Hong Guo, Ya-Jing Guo, James W Weiss, En-Sheng Ji
Obstructive sleep apnea (OSA) results in cardiac dysfunction and vascular endothelium injury. Chronic intermittent hypoxia (CIH), the main characteristic of OSAS, is considered to be mainly responsible for cardiovascular system impairment. This study is aimed to evaluate the role of endothelin-1(ET-1) system in coronary injury and cardiac dysfunction in CIH rats. In our study, Sprague-Dawley rats were exposed to CIH (FiO2 9% for 1.5 min, repeated every 3 min for 8 h/d, 7 days/week for 3 weeks). After 3 weeks, the left ventricular developed pressure (LVDP) and coronary resistance (CR) were measured with the langendorff mode in isolated hearts...
January 2017: Physiological Reports
https://www.readbyqxmd.com/read/28040057/the-effects-of-mebudipine-on-myocardial-arrhythmia-induced-by-ischemia-reperfusion-injury-in-isolated-rat-heart
#18
R Ghiasi, M Mohammadi, M Majidinia, B Yousefi, A Ghiasi, R Badalzadeh
Reperfusion of the heart after an ischemic insult may lead to potentially lethal arrhythmias and cardimyocyte cell death via apoptosis and necrosis. In addition, previous studies showed that calcium channel blockers may have a protective role in the myocardium against arrhythmia and irreversible tissue injury. Therefore, this study was aimed to investigate the effects of mebudipine on myocardial arrhythmias and tissue injury induced by ischemia/reperfusion injury in isolated rat hearts. Male Wistar rats (250‑300g) were randomly divided to Sham group (without ischemia), control group (ischemia without drug), drug group (ischemia with mebudipine 0...
November 30, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28011703/optogenetic-termination-of-ventricular-arrhythmias-in-the-whole-heart-towards-biological-cardiac-rhythm-management
#19
Emile C A Nyns, Annemarie Kip, Cindy I Bart, Jaap J Plomp, Katja Zeppenfeld, Martin J Schalij, Antoine A F de Vries, Daniël A Pijnappels
AIMS: Current treatments of ventricular arrhythmias rely on modulation of cardiac electrical function through drugs, ablation or electroshocks, which are all non-biological and rather unspecific, irreversible or traumatizing interventions. Optogenetics, however, is a novel, biological technique allowing electrical modulation in a specific, reversible and trauma-free manner using light-gated ion channels. The aim of our study was to investigate optogenetic termination of ventricular arrhythmias in the whole heart...
December 23, 2016: European Heart Journal
https://www.readbyqxmd.com/read/28006943/cardioprotection-induced-by-remote-ischemic-preconditioning-preserves-the-mitochondrial-respiratory-function-in-acute-diabetic-myocardium
#20
I Kancirová, M Jašová, M Muráriková, Z Sumbalová, O Uličná, T Ravingerová, I Waczulíková, A Ziegelhöffer, M Ferko
A 2×2 factorial design was used to evaluate possible preservation of mitochondrial functions in two cardioprotective experimental models, remote ischemic preconditioning and streptozotocin-induced diabetes mellitus, and their interaction during ischemia/reperfusion injury (I/R) of the heart. Male Wistar rats were randomly allocated into four groups: control (C), streptozotocin-induced diabetic (DM), preconditioned (RPC) and preconditioned streptozotocin-induced diabetic (DM+RPC). RPC was conducted by 3 cycles of 5-min hind-limb ischemia and 5-min reperfusion...
December 22, 2016: Physiological Research
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