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https://www.readbyqxmd.com/read/28811127/attenuation-of-sunitinib-induced-cardiotoxicity-through-the-a3-adenosine-receptor-activation
#1
Hardip Sandhu, Samantha Cooper, Afthab Hussain, Christopher Mee, Helen Maddock
Sunitinib is an anti-cancer tyrosine kinase inhibitor associated with severe cardiotoxic adverse effects. Using rat Langendorff heart model and human acute myeloid leukemia 60 (HL60) cell line we detected the involvement of protein kinase C (PKC) α during Sunitinib-induced cardiotoxicity and the effect of Sunitinib on cancer progression. The cardioprotective and anti-cancer properties of the A3 adenosine receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine-5'-N-methyluronamide (IB-MECA) were investigated...
August 12, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28801645/chronic-intermittent-hypobaric-hypoxia-improves-cardiac-function-through-inhibition-of-endoplasmic-reticulum-stress
#2
Fang Yuan, Li Zhang, Yan-Qing Li, Xu Teng, Si-Yu Tian, Xiao-Ran Wang, Yi Zhang
We investigated the role of endoplasmic reticulum stress (ERS) in chronic intermittent hypobaric hypoxia (CIHH)-induced cardiac protection. Adult male Sprague-Dawley rats were exposed to CIHH treatment simulating 5000 m altitude for 28 days, 6 hours per day. The heart was isolated and perfused with Langendorff apparatus and subjected to 30-min ischemia followed by 60-min reperfusion. Cardiac function, infarct size, and lactate dehydrogenase (LDH) activity were assessed. Expression of ERS molecular chaperones (GRP78, CHOP and caspase-12) was assayed by western blot analysis...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28766171/cisplatin-and-cisplatin-analogues-perfusion-through-isolated-rat-heart-the-effects-of-acute-application-on-oxidative-stress-biomarkers
#3
Isidora M Stojic, Vladimir I Zivkovic, Ivan M Srejovic, Tamara R Nikolic, Nevena S Jeremic, Jovana N Jeremic, Dragan M Djuric, Nemanja Jovicic, Katarina G Radonjic, Zivadin D Bugarcic, Vladimir L J Jakovljevic, Slobodan S Novokmet
Drug-induced oxidative stress can occur in numerous tissues and organ systems (liver, kidney, ear, nervous system, and cardiovascular system). Cancer therapy with cisplatin is associated with side effects to which oxidative stress may contribute. We have compared the influences of cisplatin (reference compound) and its' analogues (dichloro(1,2-diaminocyclohexane)platinum(II) and chloro(2,2':6',2″-terpyridine)platinum(II)) in a model of isolated rat heart using the Langendorff technique. The production of oxidative stress biomarkers, antioxidant enzymes, myocardial damage, and expression of Bax, OH-1, and SODs were studied...
August 1, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28758595/vasodilator-effect-of-angiotensin-1-7-on-vascular-coronary-bed-of-rats-role-of-mas-ace-and-ace2
#4
Patrícia Lanza Moraes, Lucas Miranda Kangussu, Carlos Henrique Castro, Robson As Santos, Anderson José Ferreira, Alvair P Almeida
Angiotensin(Ang)-(1-7) is a biologically active member of the renin-angiotensin system that participates of the regulation of blood pressure. Although Ang-(1-7) is able to potentiate the vasodilator effect of bradykinin in coronary bed of rats, a direct vasodilator effect of Ang-(1-7) in this vascular bed has not been characterized. Thus, the aim of this study was to evaluate the mechanisms involved in the vasodilator effect of Ang-(1-7) in the vasculature of isolated rat hearts perfused according to the Langendorff technique at constant flow...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758414/modulation-of-n-methyl-d-aspartate-receptors-in-isolated-rat-heart
#5
Ivan M Srejovic, Vladimir I Zivkovic, Tamara R Nikolic, Nevena Jeremic, Isidora Stojic, Jovana Jeremic, Dragan Djuric, Vladimir Jakovljevic
Considering the limited data about the role of N-methyl-D-aspartate receptors (NMDA-R) in cardiovascular system and heart, the aim of the present study was to examine the effects of NMDA and DL-homocysteine thiolacotne (DL-Hcy TLHC), alone and in combinations with glycine, memantine and ifenprodil, in the isolated rat heart. The hearts of Wistar albino rats were retrogradely perfused according to the Langendorff technique at a constant perfusion pressure. The experimental protocol for all experimental groups included the stabilization period, application of estimated substance in duration of 5 minutes, followed by wash-out period in duration of 10 minutes...
July 30, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28755986/investigating-the-involvement-of-glycogen-synthase-kinase-3%C3%AE-and-gap-junction-signaling-in-trpv1-and-remote-hind-preconditioning-induced-cardioprotection
#6
Puneet Kaur Randhawa, Amteshwar Singh Jaggi
Remote ischemic preconditioning (RIPC) is the phenomenon that harnesses the body's endogenous protective mechanisms against prolonged ischemia-reperfusion-induced injury. The present study aimed to explore the involvement of glycogen synthase kinase-3β and gap junction signaling in TRPV1 and remote hind preconditioning-induced cardioprotection. In the present study, four consecutive cycles (5min of ischemia-reperfusion) of remote hind limb preconditioning stimulus were delivered using a blood pressure cuff fastened at the inguinal level of the rat...
July 27, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28743285/%C3%AE-adrenergic-receptor-camp-pka-signaling-contributes-to-the-intracellular-ca-2-release-by-tentacle-extract-from-the-jellyfish-cyanea-capillata
#7
Qianqian Wang, Hui Zhang, Bo Wang, Chao Wang, Liang Xiao, Liming Zhang
BACKGROUND: Intracellular Ca(2+) overload induced by extracellular Ca(2+) entry has previously been confirmed to be an important mechanism for the cardiotoxicity as well as the acute heart dysfunction induced by jellyfish venom, while the underlying mechanism remains to be elucidated. METHODS: Under extracellular Ca(2+)-free or Ca(2+)-containing conditions, the Ca(2+) fluorescence in isolated adult mouse cardiomyocytes pre-incubated with tentacle extract (TE) from the jellyfish Cyanea capillata and β blockers was scanned by laser scanning confocal microscope...
July 25, 2017: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28733433/intensive-exercise-training-improves-cardiac-electrical-stability-in-myocardial-infarcted-rats
#8
Horesh Dor-Haim, Chaim Lotan, Michal Horowitz, Moshe Swissa
BACKGROUND: Moderate exercise training has been shown to decrease sudden cardiac death post myocardial infarction. However, the effects of intensive exercise are still controversial. METHODS AND RESULTS: Fourteen myocardial-infarcted rats were divided into sedentary (n=8) and intensive training groups (n=6) and 18 sham control rats to sedentary (n=10) and intensive training groups (n=8). Heart rate variability was obtained at weeks 1 and 8. The inducibility of ventricular tachycardia/fibrillation was assessed in a Langendorff system...
July 21, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28730389/cardiovascular-effects-of-high-molecular-weight-compounds-of-humic-nature
#9
M V Zykova, M V Belousov, T V Lasukova, A S Gorbunov, L A Logvinova, A M Dygai
The active ingredient extracted from the peat humic substances was characterized by physicochemical parameters evaluated by UV- and IR-spectroscopy, titration, and elemental (C, H, N) analysis. The cardiovascular effects of this ingredient were examined on isolated Langendorff-perfused rat heart. It was found that the active substance in a concentration range of 0.01-0.1 mg/ml produced a vasodilating effect; in addition, it decreased the end-diastolic and left-ventricular developed pressures.
June 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28725491/ds16570511-is-a-small-molecule-inhibitor-of-the-mitochondrial-calcium-uniporter
#10
Naohiro Kon, Michiko Murakoshi, Aya Isobe, Katsuji Kagechika, Naoki Miyoshi, Takahiro Nagayama
In cardiac myocytes, regulation of mitochondrial Ca(2+) is important for cellular signaling and cardiac contraction. Ca(2+) entry into the mitochondria is mediated by a highly selective Ca(2+) channel called the mitochondrial calcium uniporter, which consists of a pore-forming subunit MCU and regulatory subunits such as MICU1. Although pharmacological regulation of the mitochondrial Ca(2+) influx is a promising approach to controlling the cellular functions, a cell-permeable and specific inhibitor of the mitochondrial calcium uniporter has not yet been developed...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28721162/the-influence-of-rapamycin-on-the-early-cardioprotective-effect-of-hypoxic-preconditioning-on-cardiomyocytes
#11
Jiang Wang, YiLiyaer Maimaitili, Hong Zheng, Jin Yu, Hai Guo, Hai-Ping Ma, Chun-Ling Chen
INTRODUCTION: The purpose of this study was to examine the effects of rapamycin on the cardioprotective effect of hypoxic preconditioning (HPC) and on the mammalian target of rapamycin (mTOR)-mediated hypoxia-inducible factor 1 (HIF-1) signaling pathway. MATERIAL AND METHODS: Primary cardiomyocytes were isolated from rat pups and underwent rapamycin and/or HPC, followed by hypoxia/re-oxygenation (H/R) injury. Cell viability and cell injury were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays, and qRT-PCR was used to measure HIF-1α and mTOR mRNA expression...
June 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/28707739/angiotensin-ii-preconditioning-is-associated-with-increased-pkc%C3%AE%C2%B5-pkc%C3%AE-ratio-and-prosurvival-kinases-in-mitochondria
#12
Rebeca E Nuñez, Sabzali Javadov, Nelson Escobales
Angiotensin II preconditioning (APC) has been shown to reproduce the cardioprotective effects of ischaemic preconditioning (IPC), however, molecular mechanisms mediating the effects of APC remain unknown. In this study, Langendorff-perfused rat hearts were subjected to IPC, APC or both (IPC/APC) followed by ischaemia-reperfusion (IR), to determine translocation of PKCε, PKCδ, Akt, Erk1/2, JNK, p38 MAPK and GSK-3β to mitochondria as an indicator of activation of the protein kinases. In agreement with previous observations, IPC, APC and IPC/APC increased the recovery of left ventricular developed pressure (LVDP), reduced infarct size (IS) and lactate dehydrogenase (LDH) release, compared to controls...
July 14, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28690215/-role-of-melatonin-in-calcium-overload-induced-heart-injury
#13
Lingheng Kong, Ming Wei, Na Sun, Juanxia Zhu, Xingli Su
To investigate the role of melatonin in calcium overload-induced heart injury.
 Methods: Thirty-two rats were divided into 4 groups: a control group (Control), a melatonin control group (Mel), a calcium overload group (CaP), and a calcium overload plus melatonin group (Mel+CaP). Isolated Sprague Dawley male rat hearts underwent Langendorff perfusion. Left ventricular developed pressure (LVDP) was calculated to evaluate the myocardial performance. Triphenyltetrazolium chloride staining was used to measure the infarct size of myocardium...
June 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28686977/cardioprotective-effect-of-the-xanthones-from-gentianella-acuta-against-myocardial-ischemia-reperfusion-injury-in-isolated-rat-heart
#14
Zhibin Wang, Gaosong Wu, Hua Liu, Na Xing, Yanping Sun, Yadong Zhai, Bingyou Yang, Ah-Ng Tony Kong, Haixue Kuang, Qiuhong Wang
Gentianella acuta (Michx.) Hulten is widely used for the treatment of arrhythmia and coronary heart disease in Ewenki Folk Medicinal Plants and Mongolian Medicine, popularly known as "Wenxincao" in China. To investigate the potential protective role of the xanthones from G. acuta against myocardial I/R injury in isolated rat heart and its possible related mechanism. The protective role of xanthones on myocardial I/R injury was studied on Langendorff apparatus. The hemodynamic parameters including the left ventricular developed pressure (LVDP), the maximum rate of up/down left intraventricular pressure (±dp/dtmax), coronary flow (CF) and heart rate (HR) were recorded during the perfusion...
September 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28683206/changes-in-mitochondrial-properties-may-contribute-to-enhanced-resistance-to-ischemia-reperfusion-injury-in-the-diabetic-rat-heart
#15
Martina Muráriková, Miroslav Ferko, Iveta Waczulíková, Magdaléna Jašová, Ivana Kancirová, Jana Murínová, Táňa Ravingerová
Diabetes mellitus, besides having deleterious effects, induces cardiac adaptation that may reduce the heart's susceptibility to ischemia-reperfusion (IR) injury. This study aimed to investigate whether changes in mitochondrial properties are involved in the mechanisms of increased resistance of the diabetic heart to IR. Adult male Wistar rats were made diabetic by a single dose of streptozotocin (65 mg·kg(-1), i.p.), and on the day 8, Langendorff-perfused hearts were subjected to 30 min global ischemia and 40 min reperfusion...
August 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28676988/posttraumatic-stress-disorder-disturbs-coronary-tone-and-its-regulatory-mechanisms
#16
Svetlana S Lazuko, Olga P Kuzhel, Lyudmila E Belyaeva, Eugenia B Manukhina, H Fred Downey, Olga B Tseilikman, Maria V Komelkova, Vadim E Tseilikman
Posttraumatic stress disorder (PTSD) is associated with myocardial injury, but changes in coronary regulatory mechanisms in PTSD have not been investigated. This study evaluated the effect of PTSD-inducing stress on coronary tone and its regulation by nitric oxide (NO) and voltage-gated K(+) channels. PTSD was induced by exposing rats to predator stress, 15 min daily for 10 days, followed by 14 stress-free days. Presence of PTSD was confirmed by the elevated plus-maze test. Coronary tone was evaluated from changes in coronary perfusion pressure of Langendorff isolated hearts...
July 4, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28671970/cardioprotective-effects-of-pkg-activation-by-soluble-gc-activator-bay-60-2770-in-ischemia-reperfusion-injured-rat-hearts
#17
Kyung Hye Lee, So-Ra Lee, Haneul Cho, Jong Shin Woo, Jung Hee Kang, Yun-Mi Jeong, Xian Wu Cheng, Woo-Shik Kim, Weon Kim
Soluble guanylate cyclase (sGC) has been suggested as a therapeutic target for cardiac ischemia-reperfusion (IR) injury. Until now, the molecular mechanism of BAY 60-2770, a sGC activator, in cardiac IR injury has not been assessed. To identify the cardioprotective effects of BAY 60-2770 in IR-injured rat hearts, IR injury was established by occlusion of LAD for 40 min and reperfusion for 7 days, and the effects of BAY 60-2770 on myocardial protection were assessed by echocardiography and TTC staining. 5 nM and 5 μM of BAY 60-2770 were perfused into isolated rat hearts in a Langendorff system...
2017: PloS One
https://www.readbyqxmd.com/read/28660410/effect-of-fetal-hypothyroidism-on-myomir-network-and-its-target-gene-expression-profiles-in-heart-of-offspring-rats
#18
Nasibeh Yousefzadeh, Sajad Jeddi, Rafighe Ghiasi, Mohammad Reza Alipour
Thyroid hormone deficiency during fetal life (fetal hypothyroidism) causes intrauterine growth restriction (IUGR). Fetal hypothyroidism (FH) could attenuate normal cardiac functions in the later life of the offspring rats. The aim of this study was to evaluate the contribution of myomiR network and its target gene expression in cardiac dysfunction in fetal hypothyroid rats. Six Pregnant female rats were divided into two groups: Control consumed tap water, and the hypothyroid group received water containing 0...
June 28, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28659817/cobalt-chloride-upregulates-impaired-hif-1%C3%AE-expression-to-restore-sevoflurane-post-conditioning-dependent-myocardial-protection-in-diabetic-rats
#19
Jianjiang Wu, Long Yang, Peng Xie, Jin Yu, Tian Yu, Haiying Wang, Yiliyaer Maimaitili, Jiang Wang, Haiping Ma, Yining Yang, Hong Zheng
Previous studies from our group have demonstrated that sevoflurane post-conditioning (SPC) protects against myocardial ischemia reperfusion injury via elevating the intranuclear expression of hypoxia inducible factor-1 alpha (HIF-1α). However, diabetic SPC is associated with decreased myocardial protection and disruption of the HIF-1 signaling pathway. Previous studies have demonstrated that cobalt chloride (CoCl2) can upregulate HIF-1α expression under diabetic conditions, but whether myocardial protection by SPC can be restored afterward remains unclear...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28655690/metformin-hcl-has-curative-effect-on-rebuilding-of-ventricular-diastolic-functions-in-high-fat-diet-fed-rats
#20
Askin Ender Topal, Ilker Kelle, Hasan Akkoc, Sedat Yilmaz, Sedat Yilmaz, Murat Akkus
Myocardial lipid accumulation due to diabetes and/or obesity plays a role in the progression of left ventricular diastolic dysfunction. Our aims were to exhibit the correlation between histopathologic stage of the liver and cardiac functions, and to evaluate the effects of metformin HCl and rosiglitazone on myocardial functions. Thirty-two male Sprague-Dawley rats were divided into four groups to exhibit the correlation between histopathologic stage of the liver and cardiac functions and to determine whether metformin HCl and rosiglitazone have effects on cardiac functions...
May 2017: Pakistan Journal of Pharmaceutical Sciences
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