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https://www.readbyqxmd.com/read/28319015/collection-storage-inspection-and-quality-control-of-platelet-concentrates-obtained-by-apheresis-the-situation-in-spain
#1
Azucena Castrillo, Teresa Jimenez-Marco, José L Arroyo, María L Jurado, Luis Larrea, Rosa M Maymo, Jorge Monge, Carmen Muñoz, Ángel Pajares, Marta Yáñez
BACKGROUND: Diverse variables are involved in apheresis platelet collection, processing and storage. This survey shows how these are realized in Spain. METHOD: An analysis of collected data was performed in a questionnaire completed by ten Transfusion Centers (TC) which perform between 50 and 520 apheresis procedures per month. This information comprises the procedures used to collect, inspect and store apheresis platelet concentrates (PC), and quality control data...
February 22, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28315464/a-novel-nanosuspension-of-andrographolide-preparation-characterization-and-passive-liver-target-evaluation-in-rats
#2
Ligang Guo, Le Kang, Xinrong Liu, Xiangyun Lin, Donghua Di, Yong Wu, Dexian Kong, Yihui Deng, Yanzhi Song
Andrographolide (Andro) is an excellent anti-inflammatory bicyclic diterpene γ-lactone. However, the poor solubility limits its application as injection for the treatment of acute inflammation. To meet the clinical needs for emergency, the Andro nanosuspensions injection was first prepared by the wet milling technique. The Andro nanosuspensions were composed of 3% Andro, 5% poloxamer 188 as the non-ionic stabilizer, 0.05% sodium deoxycholate or 0.1% sodium tauroursodeoxy cholate as the ionic stabilizer, and prepared by 350rpm speed and 12cycles of grinding with 0...
March 14, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28306519/variability-in-variability-out-best-practice-recommendations-to-standardize-pre-analytical-variables-in-the-detection-of-circulating-and-tissue-micrornas
#3
Jenna Khan, Joshua A Lieberman, Christina M Lockwood
MicroRNAs (miRNAs) hold promise as biomarkers for a variety of disease processes and for determining cell differentiation. These short RNA species are robust, survive harsh treatment and storage conditions and may be extracted from blood and tissue. Pre-analytical variables are critical confounders in the analysis of miRNAs: we elucidate these and identify best practices for minimizing sample variation in blood and tissue specimens. Pre-analytical variables addressed include patient-intrinsic variation, time and temperature from sample collection to storage or processing, processing methods, contamination by cells and blood components, RNA extraction method, normalization, and storage time/conditions...
March 17, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28304094/effects-of-blood-plasma-proteins-addition-on-physico-chemical-properties-of-emulsion-type-pork-sausage-during-cold-storage
#4
Sung Ho Kim, Sang Keun Jin, Jung Seok Choi
BACKGROUND: Most of the slaughter blood is discarded, resulting in problems related to costs for wastewater disposal and environmental pollution. However, animal blood contains various proteins such as albumin, globulin and globin and can be used as a natural emulsifier, stabilizer and color additives. Thus, this study was carried out to investigate the effect of blood plasma proteins on the physico-chemical properties of emulsion-type pork sausages stored at 4 °C over 5 weeks. RESULTS: The emulsion-type pork sausages with plasma powders had higher pH than the other treatments during weeks 5, and higher shear force than the control (p < 0...
March 17, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28302909/ppn2-a-novel-zn-2-dependent-polyphosphatase-in-the-acidocalcisome-like-yeast-vacuole
#5
Rūta Gerasimaitė, Andreas Mayer
Acidocalcisome-like organelles are found in all kingdoms of life. Many of their functions, such as the accumulation and storage of metal ions, nitrogen and phosphate, the activation of blood clotting and inflammation, depend on the controlled synthesis and turnover of polyphosphate (polyP), a polymer of inorganic phosphate linked by phosphoric anhydride bonds. The exploration of the role of acidocalcisomes in metabolism and physiology requires manipulation of polyP turnover, yet the complete set of proteins responsible for this turnover is unknown...
March 16, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28302345/newborn-screening-for-six-lysosomal-storage-disorders-in-a-cohort-of-mexican-patients-three-year-findings-from-a-screening-program-in-a-closed-mexican-health-system
#6
Juana Inés Navarrete-Martínez, Ana Elena Limón-Rojas, Maria de Jesús Gaytán-García, Jesús Reyna-Figueroa, Guillermo Wakida-Kusunoki, Ma Del Rocío Delgado-Calvillo, Consuelo Cantú-Reyna, Héctor Cruz-Camino, David Eduardo Cervantes-Barragán
OBJECTIVE: To evaluate the results of a lysosomal newborn screening (NBS) program in a cohort of 20,018 Mexican patients over the course of 3years in a closed Mexican Health System (Petróleos Mexicanos [PEMEX] Health Services). STUDY DESIGN: Using dried blood spots (DBS), we performed a multiplex tandem mass spectrometry enzymatic assay for six lysosomal storage disorders (LSDs) including Pompe disease, Fabry disease, Gaucher disease, mucopolysaccharidosis type I (MPS-I), Niemann-Pick type A/B, and Krabbe disease...
March 9, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28299418/expanding-the-knowledge-on-dried-blood-spots-and-lc-ms-based-protein-analysis-two-different-sampling-materials-and-six-protein-targets
#7
Cecilie Rosting, Astrid Gjelstad, Trine Grønhaug Halvorsen
The combination of dried blood spots (DBS) and bottom-up LC-MS-based protein analysis was investigated in the present paper using six model proteins (1 mg/mL of each protein) with different physicochemical properties. Two different materials for DBS were examined: a water-soluble DBS material (carboxymethyl cellulose, (CMC)) and a commercially available (non-soluble) material (DMPK-C). The sample preparation was optimised regarding the water-soluble material and achieving acceptable repeatability of the signal was emphasised...
March 15, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28296779/efficacy-of-fresh-packed-red-blood-transfusion-in-organophosphate-poisoning
#8
Hang-Xing Bao, Pei-Jian Tong, Cai-Xia Li, Jing Du, Bing-Yu Chen, Zhi-Hui Huang, Ying Wang
The mortality rate caused by organophosphate (OP) poisoning is still high, even the standard treatment such as atropine and oxime improves a lot. To search for alternative therapies, this study was aimed to investigate the effects of packed red blood cell (RBC) transfusion in acute OP poisoning, and compare the therapeutic effects of RBCs at different storage times.Patients diagnosed with OP poisoning were included in this prospective study. Fresh RBCs (packed RBCs stored less than 10 days) and longer-storage RBCs (stored more than 10 days but less than 35 days) were randomly transfused or not into OP poisoning patients...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28295356/red-blood-cell-metabolic-responses-to-refrigerated-storage-rejuvenation-and-frozen-storage
#9
Angelo D'Alessandro, Alan D Gray, Zbignew M Sczcepiorkowski, Kirk Hansen, Louise H Herschel, Larry J Dumont
BACKGROUND: Storage of red blood cells (RBCs) under blood bank conditions promotes metabolic modulation within the RBC. This "metabolic storage lesion" may affect the quality and safety of the transfused RBCs. The aim of this study is to determine the metabolic changes in stored RBCs over 42 days of routine storage followed by a US Food and Drug Administration-approved method of rejuvenation, freezing, and preparation for transfusion. STUDY DESIGN AND METHODS: We exploited a mass spectrometry-based metabolomics approach to monitor 42-day-stored citrate phosphate dextrose/AS-1 RBCs (n = 29) that were rejuvenated, glycerolized and frozen, then thawed and deglycerolized, and held for 24 hours at 1 to 6ºC in saline-glucose...
March 10, 2017: Transfusion
https://www.readbyqxmd.com/read/28294105/breath-acetone-as-a-potential-marker-in-clinical-practice
#10
Vera Ruzsanyi, Miklós Kalapos
In recent decades, two facts have changed the opinion of researchers about the function of acetone in humans. Firstly, it has turned out that acetone cannot be regarded as simply a waste product of metabolism, because there are several pathways in which acetone is produced or broken down. Secondly, methods have emerged making possible its detection in exhaled breath, thereby offering an attractive alternative to investigation of blood and urine samples. From a clinical point of view the measurement of breath acetone levels are important, but there are limitations to its wide application...
March 15, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28293160/hemophagocytic-histiocytosis-a-clinicopathological-correlation
#11
Waseem Iqbal, Abdulaziz Ajlan Alsalloom, Khalid Shehzad, Faisal Mughal, Zafar Rasheed
OBJECTIVES: Histiocytic hyperplasia with hemophagocytosis (HP) is relatively uncommon condition that has often been mistaken in the past for neoplastic disorders. This study was conducted to investigate the possible etiology of HP, its intensity in the bone marrow (BM), and also its effect on hematological parameters with the extent of disease activity. METHODS: Blood samples were collected and BM examination was performed in 250 patients with varied etiology showing HP...
January 2017: International Journal of Health Sciences
https://www.readbyqxmd.com/read/28289733/correcting-for-microbial-blooms-in-fecal-samples-during-room-temperature-shipping
#12
Amnon Amir, Daniel McDonald, Jose A Navas-Molina, Justine Debelius, James T Morton, Embriette Hyde, Adam Robbins-Pianka, Rob Knight
The use of sterile swabs is a convenient and common way to collect microbiome samples, and many studies have shown that the effects of room-temperature storage are smaller than physiologically relevant differences between subjects. However, several bacterial taxa, notably members of the class Gammaproteobacteria, grow at room temperature, sometimes confusing microbiome results, particularly when stability is assumed. Although comparative benchmarking has shown that several preservation methods, including the use of 95% ethanol, fecal occult blood test (FOBT) and FTA cards, and Omnigene-GUT kits, reduce changes in taxon abundance during room-temperature storage, these techniques all have drawbacks and cannot be applied retrospectively to samples that have already been collected...
March 2017: MSystems
https://www.readbyqxmd.com/read/28288599/clinical-use-of-computational-modeling-for-surgical-planning-of-arteriovenous-fistula-for-hemodialysis
#13
Michela Bozzetto, Stefano Rota, Valentina Vigo, Francesco Casucci, Carlo Lomonte, Walter Morale, Massimo Senatore, Luigi Tazza, Massimo Lodi, Giuseppe Remuzzi, Andrea Remuzzi
BACKGROUND: Autogenous arteriovenous fistula (AVF) is the best vascular access (VA) for hemodialysis, but its creation is still a critical procedure. Physical examination, vascular mapping and doppler ultrasound (DUS) evaluation are recommended for AVF planning, but they can not provide direct indication on AVF outcome. We recently developed and validated in a clinical trial a patient-specific computational model to predict pre-operatively the blood flow volume (BFV) in AVF for different surgical configuration on the basis of demographic, clinical and DUS data...
March 14, 2017: BMC Medical Informatics and Decision Making
https://www.readbyqxmd.com/read/28288261/antioxidant-capacity-and-superoxide-dismutase-activity-in-adrenoleukodystrophy
#14
Bela R Turk, Benjamin E Theisen, Christina L Nemeth, Joel S Marx, Xiaohai Shi, Melissa Rosen, Richard O Jones, Ann B Moser, Paul A Watkins, Gerald V Raymond, Carol Tiffany, Ali Fatemi
Importance: X-linked adrenoleukodystrophy (ALD) may switch phenotype to the fatal cerebral form (ie, cerebral ALD [cALD]), the cause of which is unknown. Determining differences in antioxidant capacity and superoxide dismutase (SOD) levels between phenotypes may allow for the generation of a clinical biomarker for predicting the onset of cALD, as well as initiating a more timely lifesaving therapy. Objective: To identify variations in the levels of antioxidant capacity and SOD activity between ALD phenotypes in patients with cALD or adrenomyeloneuropathy (AMN), heterozygote female carriers, and healthy controls and, in addition, correlate antioxidant levels with clinical outcome scores to determine a possible predictive value...
March 13, 2017: JAMA Neurology
https://www.readbyqxmd.com/read/28287378/application-of-a-clot-based-assay-to-measure-the-procoagulant-activity-of-stored-allogeneic-red-blood-cell-concentrates
#15
Bérangère Devalet, Adeline Wannez, Nicolas Bailly, Lutfiye Alpan, Damien Gheldof, Jonathan Douxfils, Véronique Deneys, Benoît Bihin, Bernard Chatelain, Jean-Michel Dogné, Christian Chatelain, François Mullier
BACKGROUND: Thrombotic effects are possible complications of red blood cell transfusion. The generation and accumulation of procoagulant red blood cell extracellular vesicles during storage may play an important role in these thrombotic effects. The objective of this study was to assess the value of a simple phospholipid-dependent clot-based assay (STA(®)-Procoag-PPL) to estimate the procoagulant activity of stored red blood cells and changes in this activity during storage of the blood component...
March 9, 2017: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/28283474/acid-sphingomyelinase-mediates-murine-acute-lung-injury-following-transfusion-of-aged-platelets
#16
Mark John McVey, Michael Kim, Arata Tabuchi, Victoria Srbely, Lukasz Japtok, Christoph Arenz, Ori Rotstein, Burkhard Kleuser, John Wesley Semple, Wolfgang Michael Kuebler
Pulmonary complications from stored blood products are the leading cause of mortality related to transfusion. Transfusion related acute lung injury is mediated by antibodies or bioactive mediators, yet, underlying mechanisms are incompletely understood. Sphingolipids such as ceramide regulate lung injury, and their composition changes as a function of time in stored blood. Here, we tested the hypothesis that aged platelets may induce lung injury via a sphingolipid-mediated mechanism. To assess this hypothesis, a two hit mouse model was devised...
March 10, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28282803/blood-storage-alters-mechanical-stress-responses-of-erythrocytes
#17
Elif Ugurel, Zeynep Kucuksumer, Buse Eglenen, Ozlem Yalcin
BACKGROUND: Erythrocytes undergo irreversible morphological and biochemical changes during storage. Reduced levels of deformability have been reported for stored erythrocytes. Erythrocyte deformability is essential for healthy microcirculation. OBJECTIVE: The aim of this study is to evaluate shear stress (SS) induced improvements of erythrocyte deformability in stored blood. METHODS: Deformability changes were evaluated by applying physiological levels of SS (5 and 10 Pa) in metabolically depleted blood for 48 hours and stored blood for 35 days with citrate phosphate dextrose adenine-1 (CPDA-1)...
March 4, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28281231/intrinsically-disordered-regions-in-serum-albumin-what-are-they-for
#18
REVIEW
Ekaterina A Litus, Sergei E Permyakov, Vladimir N Uversky, Eugene A Permyakov
Serum albumin is a major plasma protein in mammalian blood. The importance of this protein lies in its roles in both bioregulation and transport phenomena. Serum albumin binds various metal ions and participates in the transport and storage of fatty acids, bilirubin, steroids amino acids, and many other ligands, usually with regions of hydrophobic surface. Although the primary role of serum albumin is to transport various ligand, its versatile binding capacities and high concentration mean that it can assume a number of additional functions...
March 10, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28279730/efficacy-of-aflibercept-eylea-%C3%A2-on-inhibition-of-human-vegf-in-vitro
#19
M Schicht, K Hesse, H Schröder, E Naschberger, W Lamprecht, F Garreis, F P Paulsen, L Bräuer
INTRODUCTION: Pathological formation of blood vessels plays a key role in the growth and metastasis of tumors and also in several serious ophthalmological diseases such as wet age-related macular degeneration (AMD) or diabetic retinopathy. In AMD treatment, aflibercept (tradename EYLEA(®)) is used to deactivate the underlying pathological neovascularisation. Aflibercept is a recombinant fusion protein which binds to vascular endothelial growth factor (VEGF) receptors, thereby inhibiting VEGF pathway activation...
March 7, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28278582/prolonging-the-shelf-life-of-lumbricus-terrestris-erythrocruorin-for-use-as-a-novel-blood-substitute
#20
Christine Muzzelo, Christopher Neely, Payal Shah, Osheiza Abdulmalik, Jacob Elmer
Limitations associated with the storage of red blood cells have motivated the development of novel blood substitutes that are able to withstand long-term storage at elevated temperatures. The hemoglobin of the earthworm Lumbricus terrestris (LtEc) is an attractive blood substitute candidate, since it is resistant to oxidation and aggregation during storage. Several factors were investigated to optimize the thermal and oxidative stability of LtEc during storage, including pH, antioxidant supplements, and deoxygenation...
February 20, 2017: Artificial Cells, Nanomedicine, and Biotechnology
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