keyword
MENU ▼
Read by QxMD icon Read
search

Kreiswirth

keyword
https://www.readbyqxmd.com/read/29610205/relebactam-is-a-potent-inhibitor-of-the-kpc-2-%C3%AE-lactamase-and-restores-the-susceptibility-of-imipenem-against-kpc-producing-enterobacteriaceae
#1
Krisztina M Papp-Wallace, Melissa D Barnes, Jim Alsop, Magdalena A Taracila, Christopher R Bethel, Scott A Becka, David van Duin, Barry N Kreiswirth, Keith S Kaye, Robert A Bonomo
The imipenem-relebactam combination is in development as a potential treatment regimen for infections caused by Enterobacteriaceae possessing complex β-lactamase backgrounds. Relebactam is a β-lactamase inhibitor that possesses the diazabicyclooctane core similar to avibactam, however relebactam's R1 side chain also includes a piperidine ring compared to the carboxyamide of avibactam. Here, we investigated the inactivation of Klebsiella pneumoniae carbapenemase (KPC-2), the most widespread class A carbapenemase, by relebactam and performed susceptibility testing with imipenem-relebactam using KPC-producing clinical isolates of Enterobacteriaceae...
April 2, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29535199/antibody-mediated-killing-of-carbapenem-resistant-st258-klebsiella-pneumoniae-by-human-neutrophils
#2
Scott D Kobayashi, Adeline R Porter, Brett Freedman, Ruchi Pandey, Liang Chen, Barry N Kreiswirth, Frank R DeLeo
Carbapenem-resistant Klebsiella pneumoniae is a problem worldwide. A carbapenem-resistant K. pneumoniae lineage classified as multilocus sequence type 258 (ST258) is prominent in the health care setting in many regions of the world, including the United States. ST258 strains can be resistant to virtually all clinically useful antibiotics; treatment of infections caused by these organisms is difficult, and mortality is high. As a step toward promoting development of new therapeutics for ST258 infections, we tested the ability of rabbit antibodies specific for ST258 capsule polysaccharide to enhance human serum bactericidal activity and promote phagocytosis and killing of these bacteria by human neutrophils...
March 13, 2018: MBio
https://www.readbyqxmd.com/read/29507064/pneumonia-and-renal-replacement-therapy-are-risk-factors-for-ceftazidime-avibactam-treatment-failures-and-resistance-among-patients-with-carbapenem-resistant-enterobacteriaceae-infections
#3
Ryan K Shields, M Hong Nguyen, Liang Chen, Ellen G Press, Barry N Kreiswirth, Cornelius J Clancy
Ceftazidime-avibactam was used to treat 77 patients with carbapenem-resistant Enterobacteriaceae (CRE) infections at our center. Thirty- and 90-day survival rates were 81% and 69%, respectively; rates were higher than predicted by SAPS II and SOFA scores at the onset of infection. Clinical success was achieved in 55% of patients, but varied by site of infection. Success rates were lowest for pneumonia (36%) and higher for bacteremia (75%) and urinary tract infections (88%). By multivariate analysis, pneumonia ( P =0...
March 5, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29486233/polymyxin-b-and-fosfomycin-thwart-kpc-producing-klebsiella-pneumoniae-in-the-hollow-fibre-infection-model
#4
Zackery P Bulman, Miao Zhao, Michael J Satlin, Liang Chen, Barry N Kreiswirth, Thomas J Walsh, Roger L Nation, Jian Li, Brian T Tsuji
Polymyxin B and fosfomycin are two 'old' antibiotics that consistently maintain activity against Klebsiella pneumoniae carbapenemase (KPC)-producing organisms based on in vitro susceptibility testing. However, each antibiotic's use in monotherapy has been associated with high rates of treatment failure. Therefore, our objective was to investigate the combinatorial pharmacodynamics of polymyxin B and fosfomycin against KPC-producing K. pneumoniae. Polymyxin B front-loading (3.33 mg/kg for 1 dose followed by 1...
February 24, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29463536/promoter-variation-and-gene-expression-of-mcr-1-harboring-plasmids-in-clinical-isolates-of-escherichia-coli-and-klebsiella-pneumoniae-from-a-chinese-hospital
#5
Bin Huang, Yuting He, Xingyan Ma, Renxin Cai, Jianming Zeng, Yang Lu, Weizheng Zhang, Kai Lan, Sunmei E, Yi-Wei Tang, Barry N Kreiswirth, Cha Chen, Liang Chen
Next generation sequencing of six mcr-1- harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affect the expression and promoter activity of mcr-1 , the mcr-1 gene expressions do not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.
February 20, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29440258/staphylococcal-protein-a-contributes-to-persistent-colonization-of-mice-with-staphylococcus-aureus
#6
Yan Sun, Carla Emolo, Silva Holtfreter, Siouxsie Wiles, Barry Kreiswirth, Dominique Missiakas, Olaf Schneewind
Staphylococcus aureus persistently colonizes the nasopharynx of humans, which increases the risk for invasive diseases such as skin infection and bacteremia. Nasal colonization triggers IgG responses against staphylococcal surface antigens, however these antibodies cannot prevent subsequent colonization or disease. Here we describe S. aureus WU1, a multi-locus sequence type ST88 isolate, that persistently colonizes the nasopharynx of mice. We report that staphylococcal protein A (SpA) is required for persistence of S...
February 12, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29296190/expression-characteristics-of-the-plasmid-borne-mcr-1-colistin-resistance-gene
#7
Haifang Zhang, Minhui Miao, Jieting Yan, Min Wang, Yi-Wei Tang, Barry N Kreiswirth, Xia Zhang, Liang Chen, Hong Du
The plasmid-encoded colistin resistance gene ( mcr-1 ) has recently been reported in various Gram-negative species. However, the expression profile of mcr-1 remains unknown. Here, we investigated the expression of mcr-1 in various plasmids and bacteria. The mcr-1 expression levels in pMCR1_IncX4 varied from 1.81 × 10-5 to 1.05 × 10-4 (pmol per μg total RNA) in the two K. pneumoniae strains SZ03 and SZ04 (ST25) and the two E. coli strains SZ01 and CDA6 (ST2448 and ST167, respectively). The mcr-1 expression levels of pMCR1_IncI2 in E...
December 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/29295910/range-expansion-and-the-origin-of-usa300-north-american-epidemic-methicillin-resistant-staphylococcus-aureus
#8
Lavanya Challagundla, Xiao Luo, Isabella A Tickler, Xavier Didelot, David C Coleman, Anna C Shore, Geoffrey W Coombs, Daniel O Sordelli, Eric L Brown, Robert Skov, Anders Rhod Larsen, Jinnethe Reyes, Iraida E Robledo, Guillermo J Vazquez, Raul Rivera, Paul D Fey, Kurt Stevenson, Shu-Hua Wang, Barry N Kreiswirth, Jose R Mediavilla, Cesar A Arias, Paul J Planet, Rathel L Nolan, Fred C Tenover, Richard V Goering, D Ashley Robinson
The USA300 North American epidemic (USA300-NAE) clone of methicillin-resistant Staphylococcus aureus has caused a wave of severe skin and soft tissue infections in the United States since it emerged in the early 2000s, but its geographic origin is obscure. Here we use the population genomic signatures expected from the serial founder effects of a geographic range expansion to infer the origin of USA300-NAE and identify polymorphisms associated with its spread. Genome sequences from 357 isolates from 22 U.S...
January 2, 2018: MBio
https://www.readbyqxmd.com/read/29181422/carbapenem-resistant-enterobacteriaceae-infections-in-patients-on-renal-replacement-therapy
#9
Brandon Eilertson, Eric Cober, Sandra S Richter, Federico Perez, Robert A Salata, Robert C Kalayjian, Richard R Watkins, Yohei Doi, Keith S Kaye, Scott Evans, Vance G Fowler, Robert A Bonomo, Jack DeHovitz, Barry Kreiswirth, David van Duin
Background: Patients on chronic intermittent renal replacement therapy (RRT) are at risk for infection with carbapenem-resistant Enterobacteriaceae (CRE). However, the impact of RRT on outcomes after CRE infections remains to be defined. Here we perform a comparison of outcomes for CRE-infected patients with preserved renal function compared with CRE-infected patients on RRT. Methods: Cases and controls were defined from a prospective cohort of CRE-infected patients from the Consortium on Resistance against Carbapenems in Klebsiella and other Enterobacteriaceae (CRACKLE)...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/29167294/verification-of-ceftazidime-avibactam-and-ceftolozane-tazobactam-susceptibility-testing-methods-against-carbapenem-resistant-enterobacteriaceae-and-pseudomonas-aeruginosa
#10
Ryan K Shields, Cornelius J Clancy, A William Pasculle, Ellen G Press, Ghady Haidar, Binghua Hao, Liang Chen, Barry N Kreiswirth, M Hong Nguyen
Ceftazidime-avibactam and ceftolozane-tazobactam are newly approved agents for the treatment of infections caused by multidrug-resistant Gram-negative bacteria. Resistance to both agents has been described clinically. Susceptibility testing on automated systems is unavailable for either agent. Our objective was to compare the disk diffusion and Etest methods to standard broth microdilution (BMD) methods for testing ceftazidime-avibactam and ceftolozane-tazobactam against a diverse collection of carbapenem-resistant Enterobacteriaceae (CRE) and carbapenem-resistant Pseudomonas aeruginosa (CRP) isolates, respectively...
February 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29089425/-klebsiella-pneumoniae-carbapenemase-2-kpc-2-substitutions-at-ambler-position-asp179-and-resistance-to-ceftazidime-avibactam-unique-antibiotic-resistant-phenotypes-emerge-from-%C3%AE-lactamase-protein-engineering
#11
Melissa D Barnes, Marisa L Winkler, Magdalena A Taracila, Malcolm G Page, Eric Desarbre, Barry N Kreiswirth, Ryan K Shields, Minh-Hong Nguyen, Cornelius Clancy, Brad Spellberg, Krisztina M Papp-Wallace, Robert A Bonomo
The emergence of Klebsiella pneumoniae carbapenemases (KPCs), β-lactamases that inactivate "last-line" antibiotics such as imipenem, represents a major challenge to contemporary antibiotic therapies. The combination of ceftazidime (CAZ) and avibactam (AVI), a potent β-lactamase inhibitor, represents an attempt to overcome this formidable threat and to restore the efficacy of the antibiotic against Gram-negative bacteria bearing KPCs. CAZ-AVI-resistant clinical strains expressing KPC variants with substitutions in the Ω-loop are emerging...
October 31, 2017: MBio
https://www.readbyqxmd.com/read/28947473/emergence-of-ceftolozane-tazobactam-resistant-pseudomonas-aeruginosa-during-treatment-is-mediated-by-a-single-ampc-structural-mutation
#12
Shawn H MacVane, Ruchi Pandey, Lisa L Steed, Barry N Kreiswirth, Liang Chen
Ceftolozane-tazobactam is a cephalosporin-β-lactamase inhibitor combination that exhibits potent in vitro activity against Pseudomonas aeruginosa , including strains that are resistant to other β-lactams. The emergence of ceftolozane-tazobactam resistance among clinical isolates of P. aeruginosa has rarely been described. Here we characterized ceftolozane-tazobactam-resistant P. aeruginosa strains recovered from a patient who was treated with this agent for 6 weeks for a recurrent wound infection. The results showed that the resistance was mediated by a single AmpC structural mutation...
December 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28864028/convergence-of-carbapenem-resistance-and-hypervirulence-in-klebsiella-pneumoniae
#13
Liang Chen, Barry N Kreiswirth
No abstract text is available yet for this article.
January 2018: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/28807923/pbp4-mediates-%C3%AE-lactam-resistance-by-altered-function
#14
Som S Chatterjee, Liang Chen, Aubre Gilbert, Thaina M da Costa, Vinod Nair, Sandip K Datta, Barry N Kreiswirth, Henry F Chambers
Penicillin binding protein 4 (PBP4) can provide high-level β-lactam resistance in Staphylococcus aureus A series of missense and promoter mutations associated with pbp4 were detected in strains that displayed high-level resistance. We show here that the missense mutations facilitate the β-lactam resistance mediated by PBP4 and the promoter mutations lead to overexpression of pbp4 Our results also suggest a cooperative interplay among PBPs for β-lactam resistance.
November 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28743810/polymyxin-combinations-combat-escherichia-coli-harboring-mcr-1-and-blandm-5-preparation-for-a-postantibiotic-era
#15
Zackery P Bulman, Liang Chen, Thomas J Walsh, Michael J Satlin, Yuli Qian, Jürgen B Bulitta, Charles A Peloquin, Patricia N Holden, Roger L Nation, Jian Li, Barry N Kreiswirth, Brian T Tsuji
The rapid increase of carbapenem resistance in Gram-negative bacteria has resurrected the importance of the polymyxin antibiotics. The recent discovery of plasmid-mediated polymyxin resistance (mcr-1) in carbapenem-resistant Enterobacteriaceae serves as an important indicator that the golden era of antibiotics is under serious threat. We assessed the bacterial killing of 15 different FDA-approved antibiotics alone and in combination with polymyxin B in time-killing experiments against Escherichia coli MCR1_NJ, the first reported isolate in the United States to coharbor mcr-1 and a New Delhi metallo-β-lactamase gene (blaNDM-5)...
July 25, 2017: MBio
https://www.readbyqxmd.com/read/28707004/host-sirtuin-1-regulates-mycobacterial-immunopathogenesis-and-represents-a-therapeutic-target-against-tuberculosis
#16
Catherine Y Cheng, Nuria M Gutierrez, Mardiana B Marzuki, Xiaohua Lu, Taylor W Foreman, Bhairav Paleja, Bernett Lee, Akhila Balachander, Jinmiao Chen, Liana Tsenova, Natalia Kurepina, Karen W W Teng, Kim West, Smriti Mehra, Francesca Zolezzi, Michael Poidinger, Barry Kreiswirth, Deepak Kaushal, Hardy Kornfeld, Evan W Newell, Amit Singhal
Mycobacterium tuberculosis (Mtb) executes a plethora of immune-evasive mechanisms, which contribute to its pathogenesis, limited efficacy of current therapy, and the emergence of drug-resistant strains. This has led to resurgence in attempts to develop new therapeutic strategies/targets against tuberculosis (TB). We show that Mtb down-regulates sirtuin 1 (SIRT1), a nicotinamide adenine dinucleotide (NAD(+))-dependent deacetylase, in monocytes/macrophages, TB animal models, and TB patients with active disease...
March 2017: Science Immunology
https://www.readbyqxmd.com/read/28685153/emergence-of-ceftazidime-avibactam-resistance-and-restoration-of-carbapenem-susceptibility-in-klebsiella-pneumoniae-carbapenemase-producing-k-pneumoniae-a-case-report-and-review-of-literature
#17
Ryan K Shields, M Hong Nguyen, Ellen G Press, Liang Chen, Barry N Kreiswirth, Cornelius J Clancy
We used meropenem to successfully treat a patient with bacteremia due to ceftazidime-avibactam-resistant, meropenem- susceptible Klebsiella pneumoniae that carried mutant blaKPC-3. Meropenem was bactericidal against ceftazidime-avibactam- resistant K pneumoniae isolates in vitro. Nevertheless, the role of carbapenems in treating such infections remains uncertain, because meropenem resistance is selected readily during passage experiments.
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28681808/genotypic-characterisation-of-mycobacterium-tuberculosis-isolates-from-tuberculous-meningitis-patients-at-a-tertiary-neurocare-centre-in-southern-india
#18
Akepati Chandramuki, Neelam Khanna, Elena Shashkina, Natalia Kurepina, Barun Mathema, Barry N Kreiswirth, Manjunatha M Venkataswamy
AIMS: Specific genotypes of Mycobacterium tuberculosis (MTB) have been reported to cause outbreaks of pulmonary tuberculosis (TB) in geographical areas that are endemic to TB. However, since there is little epidemiological evidence on the association of particular genotypes that cause tuberculous meningitis (TBM), we sought to investigate the association of specific MTB strains with infection of the central nervous system (CNS). MATERIALS AND METHODS: We carried out a genetic characterisation of 89 MTB isolates from TBM patients at a Southern Indian tertiary neurocare centre and compared the genotypes with strains of pulmonary TB isolated from Indian immigrants in New York City...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28630202/identifying-spectra-of-activity-and-therapeutic-niches-for-ceftazidime-avibactam-and-imipenem-relebactam-against-carbapenem-resistant-enterobacteriaceae
#19
Ghady Haidar, Cornelius J Clancy, Liang Chen, Palash Samanta, Ryan K Shields, Barry N Kreiswirth, M Hong Nguyen
We determined imipenem, imipenem-relebactam, ceftazidime, and ceftazidime-avibactam MICs against 100 CRE isolates that underwent whole-genome sequencing. Klebsiella pneumoniae carbapenemases (KPCs) were the most common carbapenemases. Forty-six isolates carried extended-spectrum β-lactamases (ESBLs). With the addition of relebactam, imipenem susceptibility increased from 8% to 88%. With the addition of avibactam, ceftazidime susceptibility increased from 0% to 85%. Neither imipenem-relebactam nor ceftazidime-avibactam was active against metallo-β-lactamase (MBL) producers...
September 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28560183/outbreak-by-hypermucoviscous-klebsiella-pneumoniae-st11-isolates-with-carbapenem-resistance-in-a-tertiary-hospital-in-china
#20
Lingling Zhan, Shanshan Wang, Yinjuan Guo, Ye Jin, Jingjing Duan, Zhihao Hao, Jingnan Lv, Xiuqin Qi, Longhua Hu, Liang Chen, Barry N Kreiswirth, Rong Zhang, Jingye Pan, Liangxing Wang, Fangyou Yu
Hypervirulent and multidrug resistant Klebsiella pneumoniae strains pose a significant threat to the public health. In the present study, 21 carbapenem-resistant K. pneumoniae isolates (CRKP) were determined by the string test as hypermucoviscous K. pneumoniae (HMKP), with the prevalence of 15.0% (21/140) among CRKP, and 1.1% (21/1838) among all K. pneumoniae isolates. Among them, 7 (33.3%), and 1 (4.76%) isolate belonged to capsular serotype K20 and K2 respectively, while 13 (61.9%, 13/21) weren't successfully typed by capsular serotyping...
2017: Frontiers in Cellular and Infection Microbiology
keyword
keyword
24166
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"