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https://www.readbyqxmd.com/read/29454832/identification-and-functional-analysis-of-inhibitor-of-nf-%C3%AE%C2%BAb-kinase-ikk-from-scylla-paramamosain-the-first-evidence-of-three-ikks-in-crab-species-and-their-expression-profiles-under-biotic-and-abiotic-stresses
#1
Mei Jiang, Dan-Dan Tu, Wen-Bin Gu, Yi-Lian Zhou, Qi-Hui Zhu, Xiao-Ling Guo, Miao-An Shu
IKK (inhibitor of NF-κB kinase) is the core regulator for NF-κB (nuclear factor-κB) pathway against pathogenic invasion in vertebrates or invertebrates. However, the IKK from crab species has not yet been identified. In the present study, three full-length cDNA sequences of IKKs from mud crab Scylla paramamosain, designated as SpIKKβ, SpIKKε1 and SpIKKε2, were firstly cloned through RT-PCR and RACE methods. This is also the first report about the identification of two IKKε genes in mud crab and even in crustaceans...
February 15, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29454268/occurrence-and-distribution-of-antibiotics-in-mariculture-farms-estuaries-and-the-coast-of-the-beibu-gulf-china-bioconcentration-and-diet-safety-of-seafood
#2
Ruiling Zhang, Jiying Pei, Ruijie Zhang, Shaopeng Wang, Weibin Zeng, Dali Huang, Yi Wang, Yuanyuan Zhang, Yinghui Wang, Kefu Yu
The occurrence, distribution, bioconcentration and diet safety via seafood consumption of 19 antibiotics were investigated in eight closed mariculture ponds, four estuaries, two nearshore areas and one offshore area from the Beibu Gulf. Seventeen, 16, 15 and 7 antibiotics were detected at total concentrations of 43.2 - 885 ng L -1 , 22.4 - 118 ng L -1 , 22.7 - 24.5 ng L -1 , and 1.81-3.23 ng L -1 in the water of the above different areas, respectively. This indicates that the mariculture ponds are important sources of antibiotic pollution on the coast of the Beibu Gulf...
February 14, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29453260/a-new-cell-extraction-method-reveals-hemolymph-microbiome-of-three-aquatic-invertebrates
#3
Xinxu Zhang, Zaiqiao Sun, Xusheng Zhang, Ming Zhang, Shengkang Li
Symbiotic microorganisms have been found in the hemolymph (blood) of many aquatic invertebrates, such as crabs, shrimps and oysters. Hemolymph is a critical site in host immune response. Currently, studies on hemolymph microorganisms are mostly performed with culture-dependent strategies using selective media (e.g., TCBS, 2216E, and LB) for enumerating and isolating microbial cells. However, doubts remain about the "true" representation of the microbial abundance and diversity of symbiotic microorganisms in hemolymph, particularly for uncultivable microorganisms which are believed to be more abundant than the cultured...
February 16, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29448932/interference-competition-as-a-key-determinant-for-spatial-distribution-of-mangrove-crabs
#4
Stefano Cannicci, Marco Fusi, Filippo Cimó, Farid Dahdouh-Guebas, Sara Fratini
BACKGROUND: The spatial distribution of mangrove crabs has been commonly associated with tree zonation and abiotic factors such as ground temperature and soil granulometry. Conversely, no studies were designed to investigate the role of competition for resources and predation in shaping crab distribution in mangroves, despite these biotic factors are recognised as key determinants for spatial patterns observed in the communities colonising rocky and sandy intertidal habitats.We studied floral and faunal assemblages in two zones of a Sri Lankan mangrove, a man-made upper intertidal level and a natural eulittoral, mid-shore one...
February 15, 2018: BMC Ecology
https://www.readbyqxmd.com/read/29438407/the-complete-mitochondrial-genome-of-somanniathelphusa-boyangensis-and-phylogenetic-analysis-of-genus-somanniathelphusa-crustacea-decapoda-parathelphusidae
#5
Xin-Nan Jia, Shu-Xin Xu, Jun Bai, Yi-Fan Wang, Zong-Heng Nie, Chun-Chao Zhu, Yan Wang, Yi-Xiong Cai, Jie-Xin Zou, Xian-Min Zhou
In this study, the authors first obtained the mitochondrial genome of Somanniathelphusa boyangensis. The results showed that the mitochondrial genome is 17,032bp in length, included 13 protein-coding genes, 2 rRNAs genes, 22 tRNAs genes and 1 putative control region, and it has the characteristics of the metazoan mitochondrial genome A+T bias. All tRNA genes display the typical clover-leaf secondary structure except tRNASer(AGN), which has lost the dihydroxyuridine arm. The GenBank database contains the mitochondrial genomes of representatives of approximately 22 families of Brachyura, comprising 56 species, including 4 species of freshwater crab...
2018: PloS One
https://www.readbyqxmd.com/read/29435229/an-anthropogenic-habitat-within-a-suboptimal-colonized-ecosystem-provides-improved-conditions-for-a-range-shifting-species
#6
Zachary J Cannizzo, Sara R Dixon, Blaine D Griffen
Many species are shifting their ranges in response to the changing climate. In cases where such shifts lead to the colonization of a new ecosystem, it is critical to establish how the shifting species itself is impacted by novel environmental and biological interactions. Anthropogenic habitats that are analogous to the historic habitat of a shifting species may play a crucial role in the ability of that species to expand or persist in suboptimal colonized ecosystems. We tested if the anthropogenic habitat of docks, a likely mangrove analog, provides improved conditions for the range-shifting mangrove tree crab Aratus pisonii within the colonized suboptimal salt marsh ecosystem...
February 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29434644/self-reported-questionnaire-survey-on-the-prevalence-and-symptoms-of-adverse-food-reactions-in-patients-with-chronic-inhalant-diseases-in-tangshan-city-china
#7
Guodong Hao, Xuxin Lai, Zhijing Song, Zhixing Wang, Xing-Ai Kong, Haifeng Zhong, Sui Fu Hui, Yiwu Zheng
Background: The prevalence of adverse food reactions in patients with chronic inhalant diseases has seldom been studied in China. This study is to investigate the prevalence of adverse food reactions and the symptoms caused in respiratory patients. Methods: Respiratory patients in allergy clinics were asked to complete a questionnaire. Patients' information such as age, gender, family history of allergy, and adverse reactions to a list of 48 foods and the symptoms caused, was recorded...
2018: Allergy, Asthma, and Clinical Immunology
https://www.readbyqxmd.com/read/29429818/undertreatment-of-older-patients-with-newly-diagnosed-multiple-myeloma-in-the-era-of-novel-therapies
#8
Bita Fakhri, Mark A Fiala, Sascha A Tuchman, Tanya M Wildes
BACKGROUND: With the expanding armamentarium of therapeutic agents for multiple myeloma (MM), it is important to identify any undertreated patient populations to mitigate outcome disparities. MATERIALS AND METHODS: We extracted the data for all plasma cell myeloma cases (International Classification of Disease for Oncology, third revision [ICD-O-3] code 9732) in the Surveillance, Epidemiology, End Results (SEER)-Medicare database from 2007 to 2011. The ICD-O-3 histologic code 9732 captures both active MM and smoldering/asymptomatic myeloma...
January 31, 2018: Clinical Lymphoma, Myeloma & Leukemia
https://www.readbyqxmd.com/read/29421084/mercury-content-of-blue-crabs-callinectes-sapidus-from-southern-new-england-coastal-habitats-contamination-in-an-emergent-fishery-and-risks-to-human-consumers
#9
David L Taylor, Nicholas M Calabrese
Total mercury (Hg; ppm dry weight) was measured in blue crabs, Callinectes sapidus, collected from Narraganset Bay and adjacent coastal lagoons and tidal rivers (Rhode Island/Massachusetts, USA) from May to August 2006-2016. For juvenile crabs (21-79mm carapace width, CW), total Hg was significantly greater in chelae muscle tissue (mean±1 SD=0.32±0.21ppm; n=65) relative to whole bodies (0.21±0.16ppm; n=19), and irrespective of tissue-type, crab Hg was positively related to CW indicating bioaccumulation of the toxicant...
January 2018: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/29414882/phcrtx2-a-new-crab-paralyzing-peptide-toxin-from-the-sea-anemone-phymanthus-crucifer
#10
Armando Alexei Rodríguez, Anoland Garateix, Emilio Salceda, Steve Peigneur, André Junqueira Zaharenko, Tirso Pons, Yúlica Santos, Roberto Arreguín, Ludger Ständker, Wolf-Georg Forssmann, Jan Tytgat, Rosario Vega, Enrique Soto
Sea anemones produce proteinaceous toxins for predation and defense, including peptide toxins that act on a large variety of ion channels of pharmacological and biomedical interest. Phymanthus crucifer is commonly found in the Caribbean Sea; however, the chemical structure and biological activity of its toxins remain unknown, with the exception of PhcrTx1, an acid-sensing ion channel (ASIC) inhibitor. Therefore, in the present work, we focused on the isolation and characterization of new P. crucifer toxins by chromatographic fractionation, followed by a toxicity screening on crabs, an evaluation of ion channels, and sequence analysis...
February 7, 2018: Toxins
https://www.readbyqxmd.com/read/29410244/a-new-host-for-hematodinium-infection-among-lithodid-crabs-from-the-sea-of-okhotsk
#11
T V Ryazanova, M G Eliseikina, A A Semenchenko
A disease caused by parasitic dinoflagellates of the genus Hematodinium has been found in the soft crab Hapalogaster grebnitzkii from the Sea of Okhotsk, which is considered a new host for this agent among lithodid crabs. This report provides macro- and micromorphological descriptions (using light and transmission electron microscopy) of the disease, as well as molecular identification of Hematodinium sp. from soft crabs, based on 18S RNA sequence data.
February 2, 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29409789/newly-identified-type-ii-crustin-spcrus2-in-scylla-paramamosain-contains-a-distinct-cysteine-distribution-pattern-exhibiting-broad-antimicrobial-activity
#12
Hui Wang, Jing-Xiao Zhang, Yue Wang, Wen-Hong Fang, Yuan Wang, Jun-Fang Zhou, Shu Zhao, Xin-Cang Li
Type II crustins are the most abundant type of crustins in shrimps that exhibit remarkable sequence diversities and broad antibacterial activities. This study characterized a novel type II crustin, SpCrus2, in the mud crab Scylla paramamosain. The SpCrus2 cDNA sequence is 620-bp long with a 495-bp open reading frame encoding a 164-amino acid protein. In the deduced protein, a 17-amino acid signal peptide, a glycine-rich hydrophobic region (GRR), and a cysteine-rich region (CRR) containing a whey acidic protein domain were predicted...
February 2, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29407802/effects-of-domestic-effluent-discharges-on-mangrove-crab-physiology-integrated-energetic-osmoregulatory-and-redox-balances-of-a-key-engineer-species
#13
Dimitri Theuerkauff, Georgina A Rivera-Ingraham, Yann Mercky, Mathilde Lejeune, Jehan-Hervé Lignot, Elliott Sucré
Mangroves are increasingly used as biofiltering systems of (pre-treated) domestic effluents. However, these wastewater discharges may affect local macrofauna. This laboratory study investigates the effects of wastewater exposure on the mangrove spider crab Neosarmatium meinerti, a key engineering species which is known to be affected by waste waters in effluent-impacted areas. These effects were quantified by monitoring biological markers of physiological state, namely oxygen consumption, the branchial cavity ventilation rate, gill physiology and morphology, and osmoregulatory and redox balance...
January 6, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29406600/non-destructive-prediction-and-visualization-of-plumpness-in-live-eriocheir-sinensis-using-low-field-1-h-magnetic-resonance-imaging
#14
Hongcai Zhang, Jun Mei, Shunsheng Chen, Xugan Wu
BACKGROUND: The plumpness of hepatopancreas and gonad tissues in live Chinese mitten crabs (Eriocheir sinensis) depends on the grading scale and its commercial value. In this work, a low-field T1 -weighted 1 H magnetic resonance imaging (LF-1 H MRI) technique was developed to non-destructively analyze the plumpness of hepatopancreas and gonad tissues in live E.sinensis. Both male and female E.sinensis were characterized by two-dimensional (2D) LF-1 H MRI. Moreover, a 3D LF-1 H MRI model that quantitatively integrated the total volume of lipid tissues in live E...
February 6, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29404841/a-multi-center-survey-on-dietary-knowledge-and-behavior-among-inpatients-in-oncology-department
#15
Minghua Cong, Jiejun Wang, Yu Fang, Yinghua Liu, Mingxiao Sun, Qiong Wu, Kan Wang, Yan Huang, Yiqun Ling, Yong Liu, Quanfu Li, Yibing Liu, Jiang Zhu, Lingjun Zhu, Zhendong Zheng, Ling Li, Dongying Liu, Zimin Liu, Hanping Shi, Peng Yuan
OBJECTIVE: To investigate the current associated factors of dietary knowledge and behavior, the intake and nutritional status in malignancy Chinese inpatients, and the malnutrition causes involved in dietary nutrition knowledge level and behavior, providing recommendations to patients for nutrition education and intervention. METHOD: Five hundred and thirty-five participants from 18 hospitals were investigated by a questionnaire related to dietary knowledge and behavior...
February 5, 2018: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
https://www.readbyqxmd.com/read/29398349/dynamics-of-energy-reserves-and-the-cost-of-reproduction-in-female-and-male-fiddler-crabs
#16
Karine D Colpo, Laura S López-Greco
The physiological costs of reproduction can be measured as the energy allocated to reproductive activities. In fiddler crabs, females allocate energy to vitellogenesis and brooding, whereas males perform expensive courtship behaviors. We evaluated in a large-scale study the reproduction cost of females and males of Leptuca uruguayensis in a temperate estuary, where their reproductive efforts are synchronized in a short reproductive season. The reproductive investments (vitellogenesis, spermatophore production, and male reproductive behaviors) were measured and related to the dynamics of storage and expenditure of energy reserves (glycogen, total lipids, and total protein) in the hepatopancreas, ovary, and muscle of the enlarged cheliped, throughout one annual cycle...
January 11, 2018: Zoology: Analysis of Complex Systems, ZACS
https://www.readbyqxmd.com/read/29395102/the-invasion-risk-of-species-associated-with-japanese-tsunami-marine-debris-in-pacific-north-america-and-hawaii
#17
Thomas W Therriault, Jocelyn C Nelson, James T Carlton, Lauran Liggan, Michio Otani, Hiroshi Kawai, Danielle Scriven, Gregory M Ruiz, Cathryn Clarke Murray
Marine debris from the Great Tsunami of 2011 represents a unique transport vector for Japanese species to reach Pacific North America and Hawaii. Here we characterize the invasion risk of invertebrate species associated with tsunami debris using a screening-level risk assessment tool - the Canadian Marine Invasive Screening Tool (CMIST). Higher-risk invertebrate invaders were identified for each of five different ecoregions. Some of these are well-known global invaders, such as the mussel Mytilus galloprovincialis and the ascidian Didemnum vexillum which already have invasion histories in some of the assessed ecoregions, while others like the sea star Asterias amurensis and the shore crab Hemigrapsus sanguineus have yet to invade large portions of the assessed ecoregions but also are recognized global invaders...
January 25, 2018: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/29390534/toe-keloid-after-nail-extraction-treated-with-surgical-excision-a-case-report
#18
Hao Liu, Kexin Song, Mingzi Zhang, Xinhang Dong, Shu Liu, Youbin Wang
RATIONALE: In this study, a case of toe keloid after nail extraction is presented, in which the keloids on both toes were resected by surgical excision. Keloids (from the Greek word meaning "crab's claw") are fibrous growths that extend beyond the original area of injury to involve the adjacent normal skin. In general, keloid tendencies appear to be regionally isolated to keloid-prone areas, such as the chest, ears, and deltoid regions, whereas the hands and feet are usually spared, which is why this case is meaningful...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29382539/molecular-cloning-and-distribution-of-oxytocin-vasopressin-like-mrna-in-the-blue-swimming-crab-portunus-pelagicus-and-its-inhibitory-effect-on-ovarian-steroid-release
#19
Jirawat Saetan, Thanapong Kruangkum, Phetcharat Phanthong, Chittipong Tipbunjong, Wandee Udomuksorn, Prasert Sobhon, Prapee Sretarugsa
This study was aimed to characterize the full length of mRNA of oxytocin/vasopressin (OT/VP)-like mRNA in female Portunus pelagicus (PpelOT/VP-like mRNA) using a partial PpelOT/VP-like sequence obtained previously in our transcriptome analysis (Saetan, 2014) to construct the primers. The PpelOT/VP-like mRNA was 626 bp long and it encoded the preprohormones containing 158 amino acids. This preprohormone consisted of a signal peptide, an active nonapeptide (CFITNCPPG) followed by the dibasic cleavage site (GKR), and the neurophysin domain...
January 27, 2018: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/29379685/molecular-phylogenetics-of-swimming-crabs-portunoidea-rafinesque-1815-supports-a-revised-family-level-classification-and-suggests-a-single-derived-origin-of-symbiotic-taxa
#20
Nathaniel Evans
Portunoidea is a diverse lineage of ecologically and economically important marine crabs comprising 8 families and 14 subfamilies. Closely related portunid subfamilies Caphyrinae and Thalamitinae constitute some of this group's greatest morphological and taxonomic diversity, and are the only known lineages to include symbiotic taxa. Emergence of symbiosis in decapods remains poorly studied and portunoid crabs provide an interesting, but often overlooked example. Yet the paucity of molecular phylogenetic data available for Portunoidea makes it challenging to investigate the evolution and systematics of the group...
2018: PeerJ
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