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https://www.readbyqxmd.com/read/28734969/integrated-analysis-of-mrna-seq-in-the-haemocytes-of-eriocheir-sinensis-in-response-to-spiroplasma-eriocheiris-infection
#1
Yinghui Wang, Yunji Xiu, Keran Bi, Jiangtao Ou, Wei Gu, Wen Wang, Qingguo Meng
The Chinese mitten crab Eriocheir sinensis is an important economic crustacean that has been exposed to various diseases. Spiroplasma eriocheiris, isolated from tremor-diseased E. sinensis, was first identified as a lethal pathogen of freshwater crustaceans. To understand the pathogenesis of S. eriocheiris to E. sinensis, the transcriptomic profiles of haemocytes in the experimental and control groups at 1 d and 7 d post-injection were obtained using Illumina HiSeq 2500. These results showed that 40,358,724, 44,462,112, 45,516,576 and 37,713,728 paired-end clean reads were obtained from the cDNA libraries of DZ1 (the control group at 1 d), DZ7 (the control group at 7 d), SY1 (the experimental group at 1 d) and SY7 (the experimental group at 7 d), respectively...
July 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28726206/preparation-of-recombinant-serpin-from-red-king-crab-paralithodes-%C3%B1-amtschaticus-for-biomedical-research-purposes
#2
N N Kostin, N A Ponomarenko, V A Isaev, G N Rudenskaya, T V Bobik, A G Gabibov, I V Smirnov
Genetic constructs with different leader sequences for intra- and extracellular expression of the target protein were generated and an original method for effective selection of clones with maximum expression was developed. For intracellular expression in the Pichia pastoris system, seprin content in cells was 6 mg/liter.
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28725411/choosing-a-mate-in-a-high-predation-environment-female-preference-in-the-fiddler-crab-uca-terpsichores
#3
Daniela M Perez, John H Christy, Patricia R Y Backwell
The interplay between a receiver's sensory system and a sender's courtship signals is fundamental to the operation of sexual selection. Male courtship signals that match a female receiver's preexisting perceptual biases can be favored yet the message they communicate is not always clear. Do they simply beacon the male's location or also indicate his quality? We explored this question in a species of fiddler crab Uca terpsichores that courts under elevated predation risk and that mates and breeds underground in the safety of males' burrows...
October 2016: Ecology and Evolution
https://www.readbyqxmd.com/read/28722104/the-double-edge-to-parasite-escape-invasive-host-is-less-infected-but-more-infectable
#4
Carolyn L Keogh, Osamu Miura, Tomohiro Nishimura, James E Byers
Non-native species that escape their native range parasites may benefit not only from reduced infection pathology, but also from relaxed selection on costly immune defenses, promoting reallocation of resources towards growth or reproduction. However, benefits accruing from a reduction in defense could come at the cost of increased infection susceptibility. We conducted common garden studies of the shore crab Hemigrapsus sanguineus from highly-parasitized native (Japan) populations and largely parasite-free invasive (USA) populations to test for differences in susceptibility to infection by native-range rhizocephalan parasites, and to explore differences in host resource allocation...
July 19, 2017: Ecology
https://www.readbyqxmd.com/read/28721835/parasites-as-prey-the-effect-of-cercarial-density-and-alternative-prey-on-consumption-of-cercariae-by-four-non-host-species
#5
Jennifer E Welsh, Caroline Liddell, Jaap VAN DER Meer, David W Thieltges
In parasites with complex life cycles the transmission of free-living infective stages can be influenced by ambient community diversity, in particular via predation. Here, we experimentally investigated whether parasite density and the presence of alternative prey can alter predation rates on free-living cercarial stages of a marine trematode by several non-host predators. All four predator species consumed increasing numbers of cercariae with an increase in cercarial density, indicating that the removal of cercariae by predators is effective over a range of natural densities as well as in the presence of alternative prey for a number of predators typical of marine ecosystems...
July 19, 2017: Parasitology
https://www.readbyqxmd.com/read/28720936/the-effects-of-superchilling-with-modified-atmosphere-packaging-on-the-physicochemical-properties-and-shelf-life-of-swimming-crab
#6
Bowen Sun, Yuanhui Zhao, Jiangang Ling, Jingfen Yu, Haitao Shang, Zunying Liu
The objective of the present study was to investigate the effects of superchilling with modified atmosphere packaging on the physicochemical properties and shelf life of swimming crab. As the storage time increased, the rates at which the total aerobic plate count, total volatile basic nitrogen, pH, peroxide value and thiobarbituric acid-reactive substances value increase were significantly lower for the superchilling with modified atmosphere packaging (SCS + MAP) treatment compared to superchilling storage (SCS) and chilling storage (CS)...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28716433/unmyelinated-peripheral-nerves-can-be-stimulated-in%C3%A2-vitro-using-pulsed-ultrasound
#7
Christopher J Wright, Seyyed R Haqshenas, John Rothwell, Nader Saffari
Appreciation for the medical and research potential of ultrasound neuromodulation is growing rapidly, with potential applications in non-invasive treatment of neurodegenerative disease and functional brain mapping spurring recent progress. However, little progress has been made in our understanding of the ultrasound-tissue interaction. The current study tackles this issue by measuring compound action potentials (CAPs) from an ex vivo crab walking leg nerve bundle and analysing the acoustic nature of successful stimuli using a passive cavitation detector (PCD)...
July 14, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28711441/emergence-of-carbapenem-resistant-acinetobacter-baumannii-st787-in-clinical-isolates-from-blood-in-a-tertiary-teaching-hospital-in-northern-taiwan
#8
Yi-Fan Hu, Charles Jia-Yin Hou, Chien-Feng Kuo, Nai-Yu Wang, Alice Ying-Jung Wu, Ching-Hsiang Leung, Chang-Pan Liu, Hung-I Yeh
BACKGROUND/PURPOSE: The purpose of this study is to investigate the predominant clones of carbapenem-resistant Acinetobacter baumannii (CRAB) in our hospital in Taiwan by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) technique. METHODS: We collected 108 non-duplicate A. baumannii clinical blood isolates from January 2012 to December 2013 in MacKay Memorial Hospital. PFGE and MLST were used for typing the A. baumannii isolates and for investigation of the predominant clones...
June 22, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28705500/development-and-characterization-of-bioactive-edible-films-from-spider-crab-maja-crispata-chitosan-incorporated-with-spirulina-extract
#9
Rafik Balti, Mohamed Ben Mansour, Nadhem Sayari, Lamia Yacoubi, Lotfi Rabaoui, Nicolas Brodu, Anthony Massé
Active food packaging films based on crab chitosan and Spirulina extract (SE) were developed. The effects of the SE incorporation at different levels on physical (color, opacity water vapor and oxygen permeability) and mechanical (tensile strength and elongation at break) properties of chitosan films were investigated. FTIR was carried out to observe the potential modifications of the chitosan films when incorporated with SE. The obtained results suggested that incorporation of SE into chitosan films improved mechanical and barrier properties...
July 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28699137/erratum-to-hepatopancreatic-cells-of-a-stone-crab-menippe-frontalis-from-per%C3%A3%C2%BA-separation-viability-study-and-evaluation-of-lipoperoxidation-against-cadmium-contamination
#10
H A Vitorino, R Y P Alta, E C P Alta, P Ortega
No abstract text is available yet for this article.
July 11, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28698237/maize-yabby-genes-drooping-leaf1-and-drooping-leaf2-regulate-plant-architecture
#11
Josh Strable, Jason G Wallace, Erica Unger-Wallace, Sarah Briggs, Peter Bradbury, Edward S Buckler, Erik Vollbrecht
Leaf architecture directly influences canopy structure, consequentially affecting yield. We discovered a maize (Zea mays) mutant with aberrant leaf architecture, which we named drooping leaf1 (drl1). Pleiotropic mutations in drl1 affect leaf length and width, leaf angle, and internode length and diameter. These phenotypes are enhanced by natural variation at the drl2 enhancer locus, including reduced expression of the drl2-Mo17 allele in the Mo17 inbred. A second drl2 allele, produced by transposon mutagenesis, interacted synergistically with drl1 mutants and reduced drl2 transcript levels...
July 11, 2017: Plant Cell
https://www.readbyqxmd.com/read/28692255/cloning-expression-and-the-effects-of-processing-on-sarcoplasmic-calcium-binding-protein-an-important-allergen-in-mud-crab
#12
Mengjun Hu, Guangyu Liu, Yang Yang, Tzu-Ming Pan, Yixiang Liu, Lechang Sun, Min-Jie Cao, Guang-Ming Liu
Shellfish allergy is a prevalent, long-lasting disorder usually persisting throughout life. However, the allergen information is incomprehensive in crab. This study aimed to identify a novel allergen in crab, show its potential in diagnosis and reduce the allergenicity by food processing. A 21-kDa protein was purified from Scylla paramamosain and confirmed as sarcoplasmic calcium binding protein (SCP) by MALDI-TOF/TOF-MS. Total RNA was isolated from crab muscle and a rapid amplification of cDNA was performed to obtain an ORF of 579 bp that coded for 193 amino acid residues...
July 10, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28692168/indirect-effects-of-ecosystem-engineering-combine-with-consumer-behavior-to-determine-the-spatial-distribution-of-herbivory
#13
Blaine D Griffen, Megan E Riley, Zachary J Cannizzo, Ilka C Feller
1.Ecosystem engineers alter environments by creating, modifying, or destroying habitats. The indirect impacts of ecosystem engineering on trophic interactions should depend on the combination of the spatial distribution of engineered structures and the foraging behavior of consumers that use these structures as refuges. 2.In this study, we assessed the indirect effects of ecosystem engineering by a wood-boring beetle in a neotropical mangrove forest system. We identified herbivory patterns in a dwarf mangrove forest on the archipelago of Twin Cays, Belize...
July 10, 2017: Journal of Animal Ecology
https://www.readbyqxmd.com/read/28690166/immune-response-of-anti-lectin-pjlec-antibody-in-freshwater-crab-paratelphusa-jacquemontii
#14
Maghil Denis, Sivakumar Mullaivanam Ramasamy, Karthigayani Thayappan, Arumugam Munusamy
Sialic acid specific lectin Pjlec isolated from serum of the freshwater crab Paratelphusa jacquemontii served as an antigen for the production of immunoglobulin (Ig) in Balb/c mice sera. Enzyme-linked immunosorbent assay (ELISA) of mice anti-sera with Pjlec lectin affirmed the induction and production of antibody. Anti-Pjlec antibody was isolated from the antisera of mice by Protein A Sepharose affinity chromatography and checked for purity by immunoblot with lectin. Mass spectrometry (MS/MS) of papain digethe peptide sequence of antigen binding fragment (Fab) and fragment crystallizable (Fc)...
July 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28690150/serotonin-induces-ecdysteroidogenesis-and-methyl-farnesoate-synthesis-in-the-mud-crab-scylla-serrata
#15
B P Girish, C H Swetha, P Sreenivasula Reddy
In the current study, we have examined the role of serotonin in regulating the levels of methyl farnesoate and ecdysteroids in the giant mud crab Scylla serrata and validated that serotonin indeed is a reproductive hormone. Administration of serotonin elevated circulatory levels of methyl farnesoate and ecdysteroids in crabs. Since methyl farnesoate and ecdysteroid act through retinoid X receptor (RXR) and ecdysteroid receptor (EcR) respectively and these receptors are involved in the regulation of reproduction in crustaceans, we have determined the mRNA levels of RXR and EcR in hepatopancreas and ovary after serotonin administration...
July 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28689716/exploring-physiological-plasticity-and-local-thermal-adaptation-in-an-intertidal-crab-along-a-latitudinal-cline
#16
Juan Diego Gaitán-Espitia, Leonardo D Bacigalupe, Tania Opitz, Nelson A Lagos, Sebastián Osores, Marco A Lardies
Intertidal organisms have evolved physiological mechanisms that enable them to maintain performance and survive during periods of severe environmental stress with temperatures close to their tolerance limits. The level of these adaptive responses in thermal physiology can vary among populations of broadly distributed species depending on their particular environmental context and genetic backgrounds. Here we examined thermal performances and reaction norms for metabolic rate (MR) and heart rate (HR) of seven populations of the porcelanid crab Petrolisthes violaceus from markedly different thermal environments across the latitudinal gradient of ~3000km...
August 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28686351/the-molting-biomarker-molecule-exists-as-2-acetamido-2-deoxy-gluconic-acid-in-urine-of-blue-crabs-and-helmet-crabs
#17
Michiya Kamio, Yasuhiro Nagakura, Hirona Yano
N-Acetyl-D-glucosamino-1,5-lactone (NAGL) 1 has been reported as a candidate component of the sex pheromone mixture of female blue crabs, Callinectes sapidus, since it is present in the urine of reproductive females and males detect it. Theoretically, 1 can convert to a 1,4-lactone isomer 2 or corresponding carboxylic acid, 2-acetamido-2-deoxy-gluconic acid 3 by hydrolysis in aqueous solution. In this study, we examined the biologically relevant state of equilibrium mixture of 1, 2 and 3 in crab urine using ESI-MS and NMR analyses...
July 7, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28684323/role-of-purified-%C3%AE-1-3-glucan-binding-protein-%C3%AE-gbp-from-paratelphusa-hydrodromus-and-their-anti-inflammatory-antioxidant-and-antibiofilm-properties
#18
Arokiadhas Iswarya, Mahalingam Anjugam, Baskaralingam Vaseeharan
β- 1, 3-glucan binding protein (β-GBP), a pattern recognition protein (PRP), plays a critical role in triggering the innate immune response by detecting β-glucan found on the surface of microbes. In the present study, β-GBP was purified from the haemolymph of rice field crab Paratelphusa hydrodromus by affinity column chromatography. The monomeric protein Ph-β-GBP appeared as a single band with a molecular weight of approximately 95 kDa in SDS-PAGE analysis and its purity was determined to be 89% by HPLC...
July 3, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28679790/the-predator-and-prey-behaviors-of-crabs-from-ecology-to-neural-adaptations
#19
REVIEW
Daniel Tomsic, Julieta Sztarker, Martín Berón de Astrada, Damián Oliva, Estela Lanza
Predator avoidance and prey capture are among the most vital of animal behaviors. They require fast reactions controlled by comparatively straightforward neural circuits often containing giant neurons, which facilitates their study with electrophysiological techniques. Naturally occurring avoidance behaviors, in particular, can be easily and reliably evoked in the laboratory, enabling their neurophysiological investigation. Studies in the laboratory alone, however, can lead to a biased interpretation of an animal's behavior in its natural environment...
July 1, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28678878/the-bigger-the-better-volume-measurements-of-parasites-and-hosts-parasitic-barnacles-cirripedia-rhizocephala-and-their-decapod-hosts
#20
Christina Nagler, Marie K Hörnig, Joachim T Haug, Christoph Noever, Jens T Høeg, Henrik Glenner
Rhizocephala, a group of parasitic castrators of other crustaceans, shows remarkable morphological adaptations to their lifestyle. The adult female parasite consists of a body that can be differentiated into two distinct regions: a sac-like structure containing the reproductive organs (the externa), and a trophic, root like system situated inside the hosts body (the interna). Parasitism results in the castration of their hosts, achieved by absorbing the entire reproductive energy of the host. Thus, the ratio of the host and parasite sizes is crucial for the understanding of the parasite's energetic cost...
2017: PloS One
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