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Kalvin cycle

Kalvin Q Tran, Antony S Tin, Gary L Firestone
Relatively little is known about the antiproliferative effects of artemisinin, a naturally occurring antimalarial compound from Artemisia annua, or sweet wormwood, in human endometrial cancer cells. Artemisinin induced a G1 cell cycle arrest in cultured human Ishikawa endometrial cancer cells and downregulated cyclin-dependent kinase-2 (CDK2) and CDK4 transcript and protein levels. Analysis of CDK4 promoter-luciferase reporter constructs showed that the artemisinin ablation of CDK4 gene expression was accounted for by the loss of CDK4 promoter activity...
March 2014: Anti-cancer Drugs
Ye Sun, Kalvin J Gregory, Nelson G Chen, Val Golovlev
A quantitative bioluminescence assay for rapid and sensitive microRNA (miRNA) expression analysis was developed. The assay uses miRNA directly as a primer for binding to a circular single-stranded DNA template, followed by rolling circle amplification. The detection of inorganic pyrophosphate (PPi) molecules released during the DNA polymerization and amplification process is performed by a multi-enzyme system. PPi is converted to ATP by ATP-sulfurylase, which provides energy for luciferase to oxidize luciferin and produce light...
October 1, 2012: Analytical Biochemistry
Antony S Tin, Shyam N Sundar, Kalvin Q Tran, Anna H Park, Kevin M Poindexter, Gary L Firestone
Artemisinin, a sesquiterpene phytolactone derived from Artemisia annua, is a potent antimalarial compound with promising anticancer properties, although the mechanism of its anticancer signaling is not well understood. Artemisinin inhibited proliferation and induced a strong G1 cell cycle arrest of cultured MCF7 cells, an estrogen-responsive human breast cancer cell line that represents an early-stage cancer phenotype, and effectively inhibited the in-vivo growth of MCF7 cell-derived tumors from xenografts in athymic nude mice...
April 2012: Anti-cancer Drugs
Crystal N Marconett, Shyam N Sundar, Min Tseng, Antony S Tin, Kalvin Q Tran, Kelly M Mahuron, Leonard F Bjeldanes, Gary L Firestone
Indole-3-carbinol (I3C), a naturally occurring hydrolysis product of glucobrassicin from cruciferous vegetables such as broccoli, cabbage and Brussels sprouts, is an anticancer phytochemical that triggers complementary sets of antiproliferative pathways to induce a cell cycle arrest of estrogen-responsive MCF7 breast cancer cells. I3C strongly downregulated transcript expression of the catalytic subunit of the human telomerase (hTERT) gene, which correlated with the dose-dependent indole-mediated G(1) cell cycle arrest without altering the transcript levels of the RNA template (hTR) for telomerase elongation...
September 2011: Carcinogenesis
Gurangad S Chandok, Kalvin K Kapoor, Rachel M Brick, Julia M Sidorova, Maria M Krasilnikova
Many mutation events in microsatellite DNA sequences were traced to the first embryonic divisions. It was not known what makes the first replication cycles of embryonic DNA different from subsequent replication cycles. Here we demonstrate that an unusual replication mode is involved in the first cycle of replication of DNA introduced in mammalian cells. This alternative replication starts at random positions, and occurs before the chromatin is fully assembled. It is detected in various cell lines and primary cells...
March 2011: Nucleic Acids Research
T P Turova, E M Spiridonova
This paper reviews phylogeny and evolution of ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) protein which is the key enzyme of the autotrophic Kalvin-Benson cycle and the most abundant protein on the planet. It consists of several structural-functional forms which include fully functional forms I, II and III catalyzing carboxylation/oxygenation of ribulose-1,5-bisphosphate and "RubisCO-like" form IV without the carboxylating activity. The genome localization, the operon structure and the copy number of the RubisCO genes varies in different autotrophic organisms...
September 2009: Molekuliarnaia Biologiia
Sylvia Daunert, Leonidas G Bachas, Vesna Schauer-Vukasinovic, Kalvin J Gregory, G Schrift, Sapna Deo
This work demonstrates the use of the protein calmodulin, CaM, as an affinity tag for the reversible immobilization of enzymes on surfaces. Our strategy takes advantage of the of the reversible, calcium-mediated binding of CaM to its ligand phenothiazine and of the ability to produce fusion proteins between CaM and a variety of enzymes to reversibly immobilize enzymes in an oriented fashion to different surfaces. Specifically, we employed two different enzymes, organophosphorus hydrolase (OPH) and beta-lactamase and two different solid supports, a silica surface and cellulose membrane modified by covalently attaching a phenothiazine ligand, to demonstrate the versatility of our immobilization method...
July 1, 2007: Colloids and Surfaces. B, Biointerfaces
R Rusev, S Donev, M Bonev, I Sivriev
On the basis of specific features of carbon reduction cycle in photosynthesis (Kalvin cycle or reduction pentosophosphate cycle) and other processes, in which triphosphoryglyceric acid (3PGA) is an intermediate product, and taking into account the available data concerning the reaction of 3PGA pool of Ch. pyrenoidosa (in vivo) to light-dark disturbances approximate kinetic curves of the value of ribulosodiphosphate (RuDP)--carboxylating reaction product and of other 3PGA accumulations in the chloroplast strom aunder light-dark disturbances...
May 1980: Biofizika
I F Karpilova, S Kerimov
The decrease in the level of NADP reduction in chloroplasts under injections of cofactors of pseudocyclic FMN photophosphorylation (vitamin K3 and methylviologen) into pea, tomato and cucumber leaves resulted in the decrease of 14CO2 autotrophic accumulation rate and in the change of distribution of assimilated carbon among the assimilation products. The inhibition of synthesis of labelled sugars and the increase of the content of 3-phosphoglyceric and glyceric acids in the labelled low molecular fraction were observed in all the experiments...
February 1978: Biokhimii︠a︡
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