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Cardiosphere derived cell

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https://www.readbyqxmd.com/read/28640894/characterization-and-analysis-of-long-non-coding-rna-lncrna-in-in-vitro-and-ex-vivo-derived-cardiac-progenitor-cells
#1
Baron Arnone, Jake Y Chen, Gangjian Qin
Recent advancements in cell-based therapies for the treatment of cardiovascular disease (CVD) show continuing promise for the use of transplanted stem and cardiac progenitor cells (CPCs) to promote cardiac restitution. However, a detailed understanding of the molecular mechanisms that control the development of these cells remains incomplete and is critical for optimizing their use in such therapy. Long non-coding (lnc) RNA has recently emerged as a crucial class of regulatory molecules involved in directing a variety of critical biological processes including development, homeostasis and disease...
2017: PloS One
https://www.readbyqxmd.com/read/28494939/cardiosphere-derived-cells-require-endoglin-for-paracrine-mediated-angiogenesis
#2
Rachael E Redgrave, Simon Tual-Chalot, Benjamin J Davison, Esha Singh, Darroch Hall, Muhammad M Amirrasouli, Derek Gilchrist, Alexander Medvinsky, Helen M Arthur
Clinical trials of stem cell therapy to treat ischemic heart disease primarily use heterogeneous stem cell populations. Small benefits occur via paracrine mechanisms that include stimulating angiogenesis, and increased understanding of these mechanisms would help to improve patient outcomes. Cardiosphere-derived-cells (CDCs) are an example of these heterogeneous stem cell populations, cultured from cardiac tissue. CDCs express endoglin, a co-receptor that binds specific transforming growth factor β (TGFβ) family ligands, including bone morphogenetic protein 9 (BMP9)...
May 9, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28435069/age-related-increase-in-wnt-inhibitor-causes-a-senescence-like-phenotype-in-human-cardiac-stem-cells
#3
Tamami Nakamura, Tohru Hosoyama, Junichi Murakami, Makoto Samura, Koji Ueno, Hiroshi Kurazumi, Ryo Suzuki, Akihito Mikamo, Kimikazu Hamano
Aging of cardiac stem/progenitor cells (CSCs) impairs heart regeneration and leads to unsatisfactory outcomes of cell-based therapies. As the precise mechanisms underlying CSC aging remain unclear, the use of therapeutic strategies for elderly patients with heart failure is severely delayed. In this study, we used human cardiosphere-derived cells (CDCs), a subtype of CSC found in the postnatal heart, to identify secreted factor(s) associated with CSC aging. Human CDCs were isolated from heart failure patients of various ages (2-83 years old)...
June 3, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28411247/exosomal-microrna-transfer-into-macrophages-mediates-cellular-postconditioning
#4
Geoffrey de Couto, Romain Gallet, Linda Cambier, Ervin Jaghatspanyan, Nupur Makkar, James Frederick Dawkins, Benjamin P Berman, Eduardo Marbán
BACKGROUND: Cardiosphere-derived cells (CDCs) confer cardioprotection in acute myocardial infarction (MI) via distinctive macrophage (MΦ) polarization. Here we demonstrate that CDC-secreted exosomes (CDCexo) recapitulate the cardioprotective effects of CDC therapy known as cellular postconditioning. METHODS: Rats and pigs underwent MI induced by ischemia-reperfusion prior to intracoronary infusion of CDCexo, inert fibroblast exosomes (Fbexo; control), or vehicle. Two days later, infarct size was quantified...
April 14, 2017: Circulation
https://www.readbyqxmd.com/read/28386845/sub-physiological-oxygen-levels-optimal-for-growth-and-survival-of-human-atrial-cardiac-stem-cells
#5
Deepthi Sreerengam RajendranNair, Jayakumar Karunakaran, Renuka R Nair
Cardiac stem cells reside in niches where the oxygen levels are close to 3%. For cytotherapy, cells are conventionally expanded in ambient oxygen (21% O2) which represents hyperoxia compared to the oxygen tension of niches. Cardiosphere-derived cells (CDCs) are then transplanted to host tissue with lower-O2 levels. The high-O2 gradient can reduce the efficacy of cultured cells. Based on the assumption that minimizing injury due to O2 gradients will enhance the yield of functionally efficient cells, CDCs were cultured in 3% O2 and compared with cells maintained in ambient O2...
April 6, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28337294/hypoxic-preconditioning-of-human-cardiosphere-derived-cell-sheets-enhances-cellular-functions-via-activation-of-the-pi3k-akt-mtor-hif-1%C3%AE-pathway
#6
Yuya Tanaka, Tohru Hosoyama, Akihito Mikamo, Hiroshi Kurazumi, Arata Nishimoto, Koji Ueno, Bungo Shirasawa, Kimikazu Hamano
Cell sheet technology is a promising therapeutic strategy for the treatment of ischemic diseases such as myocardial infarction. We recently developed a novel protocol, termed "hypoxic preconditioning," capable of augmenting the therapeutic efficacy of cell sheets. Following this protocol, the pro-angiogenic and anti-fibrotic activity of cell sheets were enhanced by brief incubation of cell sheets under hypoxic culture conditions. However, the precise molecular mechanism underlying the hypoxic preconditioning of cell sheets is unclear...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28296006/intracoronary-allogeneic-cardiosphere-derived-stem-cells-are-safe-for-use-in-dogs-with-dilated-cardiomyopathy
#7
Michael Taylor Hensley, Junnan Tang, Kathleen Woodruff, Teresa Defrancesco, Sandra Tou, Christina M Williams, Mathew Breen, Kathryn Meurs, Bruce Keene, Ke Cheng
Cardiosphere-derived cells (CDCs) have been shown to reduce scar size and increase viable myocardium in human patients with mild/moderate myocardial infarction. Studies in rodent models suggest that CDC therapy may confer therapeutic benefits in patients with non-ischaemic dilated cardiomyopathy (DCM). We sought to determine the safety and efficacy of allogeneic CDC in a large animal (canine) model of spontaneous DCM. Canine CDCs (cCDCs) were grown from a donor dog heart. Similar to human CDCs, cCDCs express CD105 and are slightly positive for c-kit and CD90...
March 15, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28205406/potency-of-human-cardiosphere-derived-cells-from-patients-with-ischemic-heart-disease-is-associated-with-robust-vascular-supportive-ability
#8
Emma Harvey, Huajun Zhang, Pilar Sepúlveda, Sara P Garcia, Dominic Sweeney, Fizzah A Choudry, Delia Castellano, George N Thomas, Hassan Kattach, Romina Petersen, Derek J Blake, David P Taggart, Mattia Frontini, Suzanne M Watt, Enca Martin-Rendon
Cardiosphere-derived cell (CDC) infusion into damaged myocardium has shown some reparative effect; this could be improved by better selection of patients and cell subtype. CDCs isolated from patients with ischemic heart disease are able to support vessel formation in vitro but this ability varies between patients. The primary aim of our study was to investigate whether the vascular supportive function of CDCs impacts on their therapeutic potential, with the goal of improving patient stratification. A subgroup of patients produced CDCs which did not efficiently support vessel formation (poor supporter CDCs), had reduced levels of proliferation and increased senescence, despite them being isolated in the same manner and having a similar immunophenotype to CDCs able to support vessel formation...
May 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28202059/cardiosphere-derived-cells-do-not-improve-cardiac-function-in-rats-with-cardiac-failure
#9
Taís Hanae Kasai-Brunswick, Andréa Rodrigues da Costa, Raiana Andrade Quintanilha Barbosa, Bruna Farjun, Fernanda Cristina Paccola Mesquita, Danúbia Silva Dos Santos, Isalira Peroba Ramos, Grazielle Suhett, Guilherme Visconde Brasil, Sandro Torrentes da Cunha, José Oscar R Brito, Juliana do Amaral Passipieri, Adriana Bastos Carvalho, Antonio Carlos Campos de Carvalho
BACKGROUND: Heart failure represents an important public health issue due to its high costs and growing incidence worldwide. Evidence showing the regenerative potential of postmitotic heart tissue has suggested the existence of endogenous cardiac stem cells in adult hearts. Cardiosphere-derived cells (CDC) constitute a candidate pool of such cardiac stem cells. Previous studies using acute myocardial infarction (MI) models in rodents demonstrated an improvement in cardiac function after cell therapy with CDC...
February 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28192031/neural-crest-stem-cells-can-differentiate-to-a-cardiomyogenic-lineage-with-an-ability-to-contract-in-response-to-pulsed-infrared-stimulation
#10
Jordan M Greenberg, Vicente Lumbreras, Daniel Pelaez, Suhrud M Rajguru, Herman S Cheung
INTRODUCTION: Cellular cardiomyoplasty has rapidly risen to prominence in the clinic following a myocardial infarction; however, low engraftment of transplanted cells limits the therapeutic benefit to these procedures. Recently, lineage-specific stem cells differentiated into cardiomyocytes have gained much attention to assist in the repair of an injured heart tissue; however, questions regarding the ideal cell source remain. In the present study, we have identified a source that is easy to extract stem cells from and show that the cells present have a high plasticity toward the cardiomyogenic lineage...
October 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28167565/y-rna-fragment-in-extracellular-vesicles-confers-cardioprotection-via-modulation-of-il-10-expression-and-secretion
#11
Linda Cambier, Geoffrey de Couto, Ahmed Ibrahim, Antonio K Echavez, Jackelyn Valle, Weixin Liu, Michelle Kreke, Rachel R Smith, Linda Marbán, Eduardo Marbán
Cardiosphere-derived cells (CDCs) reduce myocardial infarct size via secreted extracellular vesicles (CDC-EVs), including exosomes, which alter macrophage polarization. We questioned whether short non-coding RNA species of unknown function within CDC-EVs contribute to cardioprotection. The most abundant RNA species in CDC-EVs is a Y RNA fragment (EV-YF1); its relative abundance in CDC-EVs correlates with CDC potency in vivo Fluorescently labeled EV-YF1 is actively transferred from CDCs to target macrophages via CDC-EVs...
March 2017: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/28166288/correction-cardiosphere-derived-cells-facilitate-heart-repair-by-modulating-m1-m2-macrophage-polarization-and-neutrophil-recruitment
#12
Al Shaimaa Hasan, Lan Luo, Chen Yan, Tian-Xia Zhang, Yoshishige Urata, Shinji Goto, Safwat A Mangoura, Mahmoud H Abdel-Raheem, Shouhua Zhang, Tao-Sheng Li
[This corrects the article DOI: 10.1371/journal.pone.0165255.].
2017: PloS One
https://www.readbyqxmd.com/read/28158410/exosomes-secreted-by-cardiosphere-derived-cells-reduce-scarring-attenuate-adverse-remodelling-and-improve-function-in-acute-and-chronic-porcine-myocardial-infarction
#13
Romain Gallet, James Dawkins, Jackelyn Valle, Eli Simsolo, Geoffrey de Couto, Ryan Middleton, Eleni Tseliou, Daniel Luthringer, Michelle Kreke, Rachel R Smith, Linda Marbán, Bijan Ghaleh, Eduardo Marbán
No abstract text is available yet for this article.
January 14, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28129786/metformin-promotes-the-survival-of-transplanted-cardiosphere-derived-cells-thereby-enhancing-their-therapeutic-effect-against-myocardial-infarction
#14
Rongchuan Yue, Wenbin Fu, Xiang Liao, Cong Lan, Qiao Liao, Liangpeng Li, Dezhong Yang, Xuewei Xia, Xiongwen Chen, Chunyu Zeng, Wei Eric Wang
BACKGROUND: Transplantation of cardiosphere-derived cells (CDCs) has been shown to exert a therapeutic effect in patients with myocardial infarction (MI). However, poor survival of transplanted CDCs limits their beneficial effect. Metformin (MET) activates AMP-activated protein kinase (AMPK) which is associated with cell survival. The aim of this study is to determine whether MET improves CDC survival in the transplantation microenvironment and enhances the therapeutic effect of CDC transplantation against MI...
January 28, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28115549/a-discrete-in-continuous-mathematical-model-of-cardiac-progenitor-cells-formation-and-growth-as-spheroid-clusters-cardiospheres
#15
Ezio Di Costanzo, Alessandro Giacomello, Elisa Messina, Roberto Natalini, Giuseppe Pontrelli, Fabrizio Rossi, Robert Smits, Monika Twarogowska
We propose a discrete in continuous mathematical model describing the in vitro growth process of biophsy-derived mammalian cardiac progenitor cells growing as clusters in the form of spheres (Cardiospheres). The approach is hybrid: discrete at cellular scale and continuous at molecular level. In the present model, cells are subject to the self-organizing collective dynamics mechanism and, additionally, they can proliferate and differentiate, also depending on stochastic processes The two latter processes are triggered and regulated by chemical signals present in the environment...
January 23, 2017: Mathematical Medicine and Biology: a Journal of the IMA
https://www.readbyqxmd.com/read/28099883/beta2-adrenergic-signaling-affects-the-phenotype-of-human-cardiac-progenitor-cells-through-emt-modulation
#16
Francesca Pagano, Francesco Angelini, Camilla Siciliano, Julia Tasciotti, Giorgio Mangino, Elena De Falco, Roberto Carnevale, Sebastiano Sciarretta, Giacomo Frati, Isotta Chimenti
Human cardiac progenitor cells (CPCs) offer great promises to cardiac cell therapy for heart failure. Many in vivo studies have shown their therapeutic benefits, paving the way for clinical translation. The 3D model of cardiospheres (CSs) represents a unique niche-like in vitro microenvironment, which includes CPCs and supporting cells. CSs have been shown to form through a process mediated by epithelial-to-mesenchymal transition (EMT). β2-Adrenergic signaling significantly affects stem/progenitor cells activation and mobilization in multiple tissues, and crosstalk between β2-adrenergic signaling and EMT processes has been reported...
January 15, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28052915/intracoronary-cardiac-progenitor-cells-in-single-ventricle-physiology-the-perseus-cardiac-progenitor-cell-infusion-to-treat-univentricular-heart-disease-randomized-phase-2-trial
#17
RANDOMIZED CONTROLLED TRIAL
Shuta Ishigami, Shinichi Ohtsuki, Takahiro Eitoku, Daiki Ousaka, Maiko Kondo, Yoshihiko Kurita, Kenta Hirai, Yosuke Fukushima, Kenji Baba, Takuya Goto, Naohiro Horio, Junko Kobayashi, Yosuke Kuroko, Yasuhiro Kotani, Sadahiko Arai, Tatsuo Iwasaki, Shuhei Sato, Shingo Kasahara, Shunji Sano, Hidemasa Oh
RATIONALE: Patients with single ventricle physiology are at high risk of mortality resulting from ventricular dysfunction. The preliminary results of the phase 1 trial showed that cardiosphere-derived cells (CDCs) may be effective against congenital heart failure. OBJECTIVE: To determine whether intracoronary delivery of autologous CDCs improves cardiac function in patients with single ventricle physiology. METHODS AND RESULTS: We conducted a phase 2 randomized controlled study to assign in a 1:1 ratio 41 patients who had single ventricle physiology undergoing stage 2 or 3 palliation to receive intracoronary infusion of CDCs 4 to 9 weeks after surgery or staged reconstruction alone (study A)...
March 31, 2017: Circulation Research
https://www.readbyqxmd.com/read/27970213/tct-820-multivessel-intracoronary-infusion-of-allogeneic-cardiosphere-derived-cells-in-dilated-cardiomyopathy-long-term-outcomes-of-the-dilated-cardiomyopathy-intervention-with-allogeneic-myocardially-regenerative-cells-dynamic-trial
#18
Tarun Chakravarty, Raj Makkar, Timothy Henry, Michelle Kittleson, John Friedman, Daniel Berman, Joao Lima, Konstantinos Malliaras, Robert Siegel, Leandro Slipczuk, Jeff Rudy, Eduardo Marban, Janice Pogoda, Linda Marban, Deborah Ascheim
No abstract text is available yet for this article.
November 1, 2016: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/27923685/a-combined-cellular-and-surgical-ventricular-reconstruction-therapeutic-approach-produces-attenuation-of-remodeling-in-infarcted-rats
#19
Michael J Bonios, Maria Anastasiou-Nana, Despina N Perrea, Konstantinos Malliaras
BACKGROUND: Left ventricular reconstruction (LVR) has been shown to provide transient benefits to the LV structure and function of infarcted hearts; however, long-term results have been disappointing as LVR-induced benefits are typically not sustained. We hypothesized that administration of cardiosphere-derived cells (CDCs), which promote myocardial repair and regeneration, may result in long-term preservation of the beneficial effects of LVR in ischemic cardiomyopathy. METHODS: Wistar Kyoto rats underwent myocardial infarction (MI) and two weeks later were randomized into 3 groups: in Group 1 (n=9), LVR was performed by plication of the infarcted apex and CDCs were injected in the infarct border zone (IBZ); group 2 animals (n=9) underwent LVR and received vehicle solution in the IBZ; and Group 3 animals (n=10) were injected with vehicle solution in the IBZ without undergoing LVR...
December 5, 2016: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/27844250/differential-response-of-human-cardiac-stem-cells-and-bone-marrow-mesenchymal-stem-cells-to-hypoxia-reoxygenation-injury
#20
Deepthi Sreerengam RajendranNair, Jayakumar Karunakaran, Renuka R Nair
Cardiosphere-derived cells (CDCs) and bone marrow mesenchymal stem cells (MSCs) are popularly used in stem cell therapy for myocardial regeneration. The cell type that survives and maintains stem cell characteristics in the adverse microenvironment following ischemia-reperfusion injury is presumed to be ideal for transplantation. The study was therefore aimed at identifying the cell type with relatively greater resistance to ischemia-reperfusion injury. CDCs were isolated from the right atrial appendage and MSCs from bone marrow of patients who underwent coronary artery bypass graft surgery...
January 2017: Molecular and Cellular Biochemistry
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