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Cardiosphere derived cell

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https://www.readbyqxmd.com/read/29206745/cardiosphere-derived-cells-and-ischemic-heart-failure
#1
Carmel Ashur, William H Frishman
After a myocardial infarction, heart tissue becomes irreversibly damaged, leading to scar formation and inevitably ischemic heart failure. Of the many available interventions after a myocardial infarction, such as percutaneous intervention or pharmacological optimization, none can reverse the ischemic insult on the heart and restore cardiac function. Thus, the only available cure for patients with scarred myocardium is allogeneic heart transplantation, which comes with extensive costs, risks, and complications...
January 2018: Cardiology in Review
https://www.readbyqxmd.com/read/29190771/synergistic-role-of-5-azacytidine-and-ascorbic-acid-in-directing-cardiosphere-derived-cells-to-cardiomyocytes-in-vitro-by-downregulating-wnt-signaling-pathway-via-phosphorylation-of-%C3%AE-catenin
#2
Reddy Sailaja Mundre, Pavani Koka, Prakash Dhanaraj, Nitin Khatri, Sanjana Vig, Yamini Chandramohan, Anuradha Dhanasekaran
BACKGROUND: Cardiosphere derived cells (CDCs) represent a valuable source in stem cell based therapy for cardiovascular diseases, yet poor differentiation rate hinders the transplantation efficiency. The aim of this study is to check the ability of 5-Azacytidine (Aza) alone and in combination with ascorbic acid (Aza+AA) in delineating CDCs to cardiomyogenesis and the underlying Wnt signaling mechanism in induced differentiation. METHODS: CDCs were treated with Aza and Aza+AA for a period of 14 days to examine the expression of cardiac specific markers and Wnt downstream regulators by immunofluorescence, real time PCR and western blot...
2017: PloS One
https://www.readbyqxmd.com/read/29104594/sex-differences-of-human-cardiac-progenitor-cells-in-the-biological-response-to-tnf-%C3%AE-treatment
#3
Elisabetta Straface, Lucrezia Gambardella, Francesca Pagano, Francesco Angelini, Barbara Ascione, Rosa Vona, Elena De Falco, Elena Cavarretta, Raffaele La Russa, Walter Malorni, Giacomo Frati, Isotta Chimenti
Adult cardiac progenitor cells (CPCs), isolated as cardiosphere-derived cells (CDCs), represent promising candidates for cardiac regenerative therapy. CDCs can be expanded in vitro manyfolds without losing their differentiation potential, reaching numbers that are appropriate for clinical applications. Since mechanisms of successful CDC survival and engraftment in the damaged myocardium are still critical and unresolved issues, we aimed at deciphering possible key factors capable of bolstering CDC function...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29020403/cardiac-and-systemic-rejuvenation-after-cardiosphere-derived-cell-therapy-in-senescent-rats
#4
Lilian Grigorian-Shamagian, Weixin Liu, Soraya Fereydooni, Ryan C Middleton, Jackelyn Valle, Jae Hyung Cho, Eduardo Marbán
Aim: The aim is to assess the effects of CDCs on heart structure, function, gene expression, and systemic parameters in aged rats. Diastolic dysfunction is characteristic of aged hearts. Cardiosphere-derived cell (CDC) therapy has exhibited several favourable effects on heart structure and function in humans and in preclinical models; however, the effects of CDCs on aging have not been evaluated. Methods and Results: We compared intra-cardiac injections of neonatal rat CDCs to vehicle (phosphate-buffered saline, PBS) in 21...
October 14, 2017: European Heart Journal
https://www.readbyqxmd.com/read/29016556/the-current-status-and-future-of-cardiac-stem-progenitor-cell-therapy-for-congenital-heart-defects-from-diabetic-pregnancy
#5
REVIEW
Jianxiang Zhong, Shengbing Wang, Wei-Bin Shen, Sunjay Kaushal, Peixin Yang
Pregestational maternal diabetes induces congenital heart defects (CHDs). Cardiac dysfunction after palliative surgical procedures contributes to the high mortality of CHD patients. Autologous or allogeneic stem cell therapies are effective for improving cardiac function in animal models and clinical trials. c-kit(+) cardiac progenitor cells (CPCs), the most recognized CPCs, have the following basic properties of stem cells: self-renewal, multicellular clone formation, and differentiation into multiple cardiac lineages...
October 10, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28857080/cardioprotection-anti-ageing-effects-of-cardiosphere-derived-cells
#6
Irene Fernández-Ruiz
No abstract text is available yet for this article.
August 31, 2017: Nature Reviews. Cardiology
https://www.readbyqxmd.com/read/28837618/therapeutic-benefits-of-intravenous-cardiosphere-derived-cell-therapy-in-rats-with-pulmonary-hypertension
#7
Ryan C Middleton, Mario Fournier, Xuan Xu, Eduardo Marbán, Michael I Lewis
Pulmonary arterial hypertension (PAH) is a progressive condition characterized by occlusive pulmonary arteriopathy, in which survival remains poor despite pharmacologic advances. The aim of this study was to evaluate the ability of cardiosphere-derived cells (CDCs), cardiac progenitor cells with potent anti-inflammatory and immunomodulatory properties, to attenuate hemodynamic and morphometric remodeling of the right ventricle (RV) and pulmonary arterioles in rats with established monocrotaline (MCT)-induced PAH...
2017: PloS One
https://www.readbyqxmd.com/read/28834400/cryopreservation-of-canine-cardiosphere-derived-cells-implications-for-clinical-application
#8
Luke C Dutton, Sophie A V Church, Hannah Hodgkiss-Geere, Brian Catchpole, Anthony Huggins, Jayesh Dudhia, David J Connolly
The clinical application of cardiosphere-derived cells (CDCs) to treat cardiac disease has gained increasing interest over the past decade. Recent clinical trials confirm their regenerative capabilities, although much remains to be elucidated about their basic biology. To develop this new treatment modality, in a cost effective and standardized workflow, necessitates the creation of cryopreserved cell lines to facilitate access for cardiac patients requiring urgent therapy. Cryopreservation may however lead to alterations in cell behavior and potency...
August 22, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28815002/a-comprehensive-method-for-identification-of-suitable-reference-genes-in-extracellular-vesicles
#9
Kenneth Gouin, Kiel Peck, Travis Antes, Jennifer Leigh Johnson, Chang Li, Sharon Denise Vaturi, Ryan Middleton, Geoff de Couto, Ann-Sophie Walravens, Luis Rodriguez-Borlado, Rachel Ruckdeschel Smith, Linda Marbán, Eduardo Marbán, Ahmed Gamal-Eldin Ibrahim
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is one of the most sensitive, economical and widely used methods for evaluating gene expression. However, the utility of this method continues to be undermined by a number of challenges including normalization using appropriate reference genes. The need to develop tailored and effective strategies is further underscored by the burgeoning field of extracellular vesicle (EV) biology. EVs contain unique signatures of small RNAs including microRNAs (miRs)...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28740514/normal-versus-pathological-cardiac-fibroblast-derived-extracellular-matrix-differentially-modulates-cardiosphere-derived-cell-paracrine-properties-and-commitment
#10
Francesca Pagano, Francesco Angelini, Clotilde Castaldo, Vittorio Picchio, Elisa Messina, Sebastiano Sciarretta, Ciro Maiello, Giuseppe Biondi-Zoccai, Giacomo Frati, Franca di Meglio, Daria Nurzynska, Isotta Chimenti
Human resident cardiac progenitor cells (CPCs) isolated as cardiosphere-derived cells (CDCs) are under clinical evaluation as a therapeutic product for cardiac regenerative medicine. Unfortunately, limited engraftment and differentiation potential of transplanted cells significantly hamper therapeutic success. Moreover, maladaptive remodelling of the extracellular matrix (ECM) during heart failure progression provides impaired biological and mechanical signals to cardiac cells, including CPCs. In this study, we aimed at investigating the differential effect on the phenotype of human CDCs of cardiac fibroblast-derived ECM substrates from healthy or diseased hearts, named, respectively, normal or pathological cardiogel (CG-N/P)...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28722223/the-role-of-%C3%AE-adrenergic-receptors-and-p38mapk-signaling-pathways-in-physiological-processes-of-cardiosphere-derived-cells
#11
Wen-Bo Zhang, Yun-Qi Liu, Xi Zhang, Lin Lin, Sheng-Li Yin
The effects of β adrenergic receptors (β-ARs) and p38 mitogen-activated protein kinases (MAPK) pathways on cardiosphere-derived cells (CDCs) are largely unknown. This study aimed to investigate the roles of β-ARs and p38MAPK pathways on the proliferation, apoptosis, and differentiation capacity of CDCs. The CDCs were treated with β1-AR blocker (Met group), β2-AR antagonist (ICI group), and p38MAPK inhibitor (SB group), non-selective β-AR blocker (PRO group), and β-AR agonist (ISO group). The viability, apoptotic rate and differentiation status of CDCs were determined by MST-1 assay, flow cytometery, and Western blot, respectively...
July 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28674094/is-cardioprotection-dead
#12
REVIEW
David J Lefer, Eduardo Marbán
For >4 decades, the holy grail in the treatment of acute myocardial infarction has been the mitigation of lethal injury. Despite promising initial results and decades of investigation by the cardiology research community, the only treatment with proven efficacy is early reperfusion of the occluded coronary artery. The remarkable record of failure has led us and others to wonder if cardioprotection is dead. The path to translation, like the ascent to Everest, is certainly littered with corpses. We do, however, highlight a therapeutic principle that provides a glimmer of hope: cellular postconditioning...
July 4, 2017: Circulation
https://www.readbyqxmd.com/read/28640894/characterization-and-analysis-of-long-non-coding-rna-lncrna-in-in-vitro-and-ex-vivo-derived-cardiac-progenitor-cells
#13
Baron Arnone, Jake Y Chen, Gangjian Qin
Recent advancements in cell-based therapies for the treatment of cardiovascular disease (CVD) show continuing promise for the use of transplanted stem and cardiac progenitor cells (CPCs) to promote cardiac restitution. However, a detailed understanding of the molecular mechanisms that control the development of these cells remains incomplete and is critical for optimizing their use in such therapy. Long non-coding (lnc) RNA has recently emerged as a crucial class of regulatory molecules involved in directing a variety of critical biological processes including development, homeostasis and disease...
2017: PloS One
https://www.readbyqxmd.com/read/28494939/cardiosphere-derived-cells-require-endoglin-for-paracrine-mediated-angiogenesis
#14
Rachael E Redgrave, Simon Tual-Chalot, Benjamin J Davison, Esha Singh, Darroch Hall, Muhammad M Amirrasouli, Derek Gilchrist, Alexander Medvinsky, Helen M Arthur
Clinical trials of stem cell therapy to treat ischemic heart disease primarily use heterogeneous stem cell populations. Small benefits occur via paracrine mechanisms that include stimulating angiogenesis, and increased understanding of these mechanisms would help to improve patient outcomes. Cardiosphere-derived-cells (CDCs) are an example of these heterogeneous stem cell populations, cultured from cardiac tissue. CDCs express endoglin, a co-receptor that binds specific transforming growth factor β (TGFβ) family ligands, including bone morphogenetic protein 9 (BMP9)...
May 9, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28435069/age-related-increase-in-wnt-inhibitor-causes-a-senescence-like-phenotype-in-human-cardiac-stem-cells
#15
Tamami Nakamura, Tohru Hosoyama, Junichi Murakami, Makoto Samura, Koji Ueno, Hiroshi Kurazumi, Ryo Suzuki, Akihito Mikamo, Kimikazu Hamano
Aging of cardiac stem/progenitor cells (CSCs) impairs heart regeneration and leads to unsatisfactory outcomes of cell-based therapies. As the precise mechanisms underlying CSC aging remain unclear, the use of therapeutic strategies for elderly patients with heart failure is severely delayed. In this study, we used human cardiosphere-derived cells (CDCs), a subtype of CSC found in the postnatal heart, to identify secreted factor(s) associated with CSC aging. Human CDCs were isolated from heart failure patients of various ages (2-83 years old)...
June 3, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28411247/exosomal-microrna-transfer-into-macrophages-mediates-cellular-postconditioning
#16
Geoffrey de Couto, Romain Gallet, Linda Cambier, Ervin Jaghatspanyan, Nupur Makkar, James Frederick Dawkins, Benjamin P Berman, Eduardo Marbán
BACKGROUND: Cardiosphere-derived cells (CDCs) confer cardioprotection in acute myocardial infarction by distinctive macrophage (Mϕ) polarization. Here we demonstrate that CDC-secreted exosomes (CDCexo) recapitulate the cardioprotective effects of CDC therapy known as cellular postconditioning. METHODS: Rats and pigs underwent myocardial infarction induced by ischemia/reperfusion before intracoronary infusion of CDCexo, inert fibroblast exosomes (Fbexo; control), or vehicle...
July 11, 2017: Circulation
https://www.readbyqxmd.com/read/28386845/sub-physiological-oxygen-levels-optimal-for-growth-and-survival-of-human-atrial-cardiac-stem-cells
#17
Deepthi Sreerengam RajendranNair, Jayakumar Karunakaran, Renuka R Nair
Cardiac stem cells reside in niches where the oxygen levels are close to 3%. For cytotherapy, cells are conventionally expanded in ambient oxygen (21% O2) which represents hyperoxia compared to the oxygen tension of niches. Cardiosphere-derived cells (CDCs) are then transplanted to host tissue with lower-O2 levels. The high-O2 gradient can reduce the efficacy of cultured cells. Based on the assumption that minimizing injury due to O2 gradients will enhance the yield of functionally efficient cells, CDCs were cultured in 3% O2 and compared with cells maintained in ambient O2...
August 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28337294/hypoxic-preconditioning-of-human-cardiosphere-derived-cell-sheets-enhances-cellular-functions-via-activation-of-the-pi3k-akt-mtor-hif-1%C3%AE-pathway
#18
Yuya Tanaka, Tohru Hosoyama, Akihito Mikamo, Hiroshi Kurazumi, Arata Nishimoto, Koji Ueno, Bungo Shirasawa, Kimikazu Hamano
Cell sheet technology is a promising therapeutic strategy for the treatment of ischemic diseases such as myocardial infarction. We recently developed a novel protocol, termed "hypoxic preconditioning," capable of augmenting the therapeutic efficacy of cell sheets. Following this protocol, the pro-angiogenic and anti-fibrotic activity of cell sheets were enhanced by brief incubation of cell sheets under hypoxic culture conditions. However, the precise molecular mechanism underlying the hypoxic preconditioning of cell sheets is unclear...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28296006/intracoronary-allogeneic-cardiosphere-derived-stem-cells-are-safe-for-use-in-dogs-with-dilated-cardiomyopathy
#19
Michael Taylor Hensley, Junnan Tang, Kathleen Woodruff, Teresa Defrancesco, Sandra Tou, Christina M Williams, Mathew Breen, Kathryn Meurs, Bruce Keene, Ke Cheng
Cardiosphere-derived cells (CDCs) have been shown to reduce scar size and increase viable myocardium in human patients with mild/moderate myocardial infarction. Studies in rodent models suggest that CDC therapy may confer therapeutic benefits in patients with non-ischaemic dilated cardiomyopathy (DCM). We sought to determine the safety and efficacy of allogeneic CDC in a large animal (canine) model of spontaneous DCM. Canine CDCs (cCDCs) were grown from a donor dog heart. Similar to human CDCs, cCDCs express CD105 and are slightly positive for c-kit and CD90...
August 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28205406/potency-of-human-cardiosphere-derived-cells-from-patients-with-ischemic-heart-disease-is-associated-with-robust-vascular-supportive-ability
#20
Emma Harvey, Huajun Zhang, Pilar Sepúlveda, Sara P Garcia, Dominic Sweeney, Fizzah A Choudry, Delia Castellano, George N Thomas, Hassan Kattach, Romina Petersen, Derek J Blake, David P Taggart, Mattia Frontini, Suzanne M Watt, Enca Martin-Rendon
Cardiosphere-derived cell (CDC) infusion into damaged myocardium has shown some reparative effect; this could be improved by better selection of patients and cell subtype. CDCs isolated from patients with ischemic heart disease are able to support vessel formation in vitro but this ability varies between patients. The primary aim of our study was to investigate whether the vascular supportive function of CDCs impacts on their therapeutic potential, with the goal of improving patient stratification. A subgroup of patients produced CDCs which did not efficiently support vessel formation (poor supporter CDCs), had reduced levels of proliferation and increased senescence, despite them being isolated in the same manner and having a similar immunophenotype to CDCs able to support vessel formation...
May 2017: Stem Cells Translational Medicine
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