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https://www.readbyqxmd.com/read/28972863/diversity-of-class-1-integrons-and-disruption-of-caro-and-dacd-by-insertion-sequences-among-acinetobacter-baumannii-isolates-in-tehran-iran
#1
Maryam Mirshekar, Fereshteh Shahcheraghi, Omid Azizi, Hamid Solgi, Farzad Badmasti
Acinetobacter baumannii is an important nosocomial pathogen which causes a wide range of infections. In this study, we addressed the role of class 1 integron, ISAba1 and ISAba125 associated with antimicrobial resistance in 72 clinical isolates of A. baumannii collected from clinical settings in Tehran, Iran. Moreover, to study the clonal relatedness of strains, repetitive extragenic palindromic-PCR (rep-PCR) assay was carried out. PCR revealed that blaOXA-51-like, blaOXA-23-like, blaOXA-24/40-like, blaOXA-58-like, blaNDM, integrase gene (intI1), ISAba1, and ISAba125 were present in 86...
October 3, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28887202/hospital-sewage-water-a-reservoir-for-variants-of-new-delhi-metallo-%C3%AE-lactamase-blandm-and-esbl-producing-enterobacteriaceae
#2
Shadab Parvez, Asad U Khan
New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae has become a threat to public health. Hospital sewage is generally unexplored, having a potential for harbouring bacteria causing healthcare-associated infections. Hence, we initiated this study to monitor NDM-producing Enterobacteriaceae in hospital sewage water. We detected 32 isolates with blaNDM variants, 17 Escherichia coli, 8 Citrobacter freundii, 4 Shigella boydii, 2 Citrobacter braakii, and 1 Citrobacter farmeri in hospital sewage water, showing resistance to all antibiotics except colistin...
September 5, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28876169/ndm-1-hazard-in-the-balkan-states-evidence-of-the-first-outbreak-of-ndm-1-producing-klebsiella-pneumoniae-in-bulgaria
#3
Encho Savov, Lida Politi, Nicholas Spanakis, Angelina Trifonova, Elena Kioseva, Athanasios Tsakris
New Delhi MBL (NDM) carbapenemase-producing Klebsiella pneumoniae has become one of the most concerning multidrug-resistant pathogens. The Balkan counties are considered a reservoir for the spread of such strains based on several reports documenting NDM infections after hospitalization in this region. Nevertheless, NDM-producing K. pneumoniae have been only occasionally documented from Balkans. The current study documents the first polyclonal outbreak caused by NDM-1-producing K. pneumoniae in Bulgaria. From July 2015 to April 2016, all 25 single-patient carbapenem-nonsusceptible K...
September 6, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28676284/incx3-plasmid-mediated-occurrence-of-blandm-4-within-escherichia-coli-st448-from-india
#4
Nargis Alom Choudhury, Deepjyoti Paul, Atanu Chakravarty, Amitabha Bhattacharjee, Debadatta Dhar Chanda
This study was designed to investigate blaNDM-4 encoded within IncX3 type plasmid and their copy number alteration under carbapenem pressure within clinical isolates of Escherichia coli. NDM-4 producing E. coli isolates were collected from an Indian hospital and transferability as well as plasmid incompatibility typing was determined. Genetic environment and antibiogram profiling was carried out. Quantitative Real Time PCR was done to determine the change in plasmid copy number under concentration gradient carbapenem stress...
July 1, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28650275/first-description-of-blandm-7-carried-on-an-incx4-plasmid-in-escherichia-coli-st679-isolated-in-spain
#5
Paula Espinal, Elisenda Miró, Concepción Segura, Laura Gómez, Virginia Plasencia, Pere Coll, Ferran Navarro
This study describes the molecular characterization of an NDM-7 carbapenemase-producing Escherichia coli strain Ec188, recovered from a rectal swab of a male patient who had travelled to Pakistan before his hospitalization at the Hospital del Mar in Barcelona, Spain. The Ec188 isolate, assigned to a new multilocus sequence type ST679, was resistant to all beta-lactams, aminoglycosides (gentamicin, tobramycin, and with reduced susceptibility to amikacin), and ciprofloxacin. The blaNDM-7 gene was located on a 50 kb IncX4 plasmid (pEc188-NDM7), both in the original and transconjugant strains...
June 26, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28613981/detection-of-new-delhi-metallo-%C3%AE-lactamase-variants-ndm-4-ndm-5-and-ndm-7-in-enterobacter-aerogenes-isolated-from-a-neonatal-intensive-care-unit-of-a-north-india-hospital-a-first-report
#6
Nayeem Ahmad, Syed Manazir Ali, Asad U Khan
A total 402 Enterobacteriaceae isolates were recovered from blood and rectal swabs of 1,000 infants admitted to the Neonatal Intensive Care Unit (NICU) of the Jawaharlal Medical College and Hospital Aligarh, India. Carbapenamase producers were determined by Carba NP phenotype biochemical assay. Out of 402 isolates, it was the first time three of the isolates were identified as Enterobacter aerogenes carrying blaNDM-4, blaNDM-5, and blaNDM-7 genes. These genes were identified by polymerase chain reaction (PCR) and sequence analysis...
June 14, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28594634/dissemination-of-blaoxa-23-blaoxa-24-blaoxa-58-and-blandm-1-genes-of-acinetobacter-baumannii-isolates-from-four-tertiary-hospitals-in-thailand
#7
Udomluk Leungtongkam, Rapee Thummeepak, Suchada Wongprachan, Pollawat Thongsuk, Thawatchai Kitti, Kwanjai Ketwong, Chakkaphan Runcharoen, Narisara Chantratita, Sutthirat Sitthisak
Acinetobacter baumannii is a major threat to public health due to the emergence and dissemination of antibiotic-resistant strains. The purpose of this study was to determine the molecular epidemiology of antibiotic-resistant A. baumannii isolates collected from four tertiary hospitals in Thailand during the period November 2013-February 2015. We screened 339 A. baumannii, nonrepetitive clinical isolates to determine drug susceptibility. Among all isolates, we found that 7.9% was nondrug-resistant A. baumannii (NR-AB)...
June 8, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28506721/first-report-of-ndm-1-producing-clinical-isolate-of-leclercia-adecarboxylata-in-spain
#8
Cristina Riazzo, Lorena López-Cerero, María Dolores Rojo-Martín, Yannick Hoyos-Mallecot, Felipe Fernández-Cuenca, Jose Luis Martín-Ruíz, Álvaro Pascual-Hernández, Thierry Naas, Jose María Navarro-Marí
We describe the case of NDM-1-producing Leclercia adecarboxylata recovered from the clinical sample of a patient hospitalized for a trauma-related injury to his foot. The isolate was resistant to all beta-lactams, quinolones, trimetroprim-sulfametoxazol, gentamicin and tobramicyn. The blaNDM-1 gene was located in a conjugative plasmid that also contained the blaSHV-12 gene and was preceded by a disrupted insertion sequence of ISAba125. The plasmid belongs to the incompatibility group X3, which is known to be an important vector for NDM-1 dissemination in China...
May 3, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28193905/assessment-of-insertion-sequence-mobilization-as-an-adaptive-response-to-oxidative-stress-in-acinetobacter-baumannii-using-is-seq
#9
Meredith S Wright, Stephanie Mountain, Karen Beeri, Mark D Adams
Insertion sequence (IS) elements are found throughout bacterial genomes and contribute to genome variation by interrupting genes or altering gene expression. Few of the more than 30 IS elements described in Acinetobacter baumannii have been characterized for transposition activity or expression effects. A targeted sequencing method, IS-seq, was developed to efficiently map the locations of new insertion events in A. baumannii genomes and was used to identify novel IS sites following growth in the presence of hydrogen peroxide, which causes oxidative stress...
May 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28191309/co-existence-of-blaoxa-23-and-blandm-1-genes-of-acinetobacter-baumannii-isolated-from-nepal-antimicrobial-resistance-and-clinical-significance
#10
Prabhu Raj Joshi, Mahesh Acharya, Trishna Kakshapati, Udomluk Leungtongkam, Rapee Thummeepak, Sutthirat Sitthisak
BACKGROUND: Molecular analysis of carbapenem-resistant genes in Acinetobacter baumannii, an emerging pathogen, is less commonly reported from Nepal. In this study we determined the antibiotic susceptibility profile and genetic mechanism of carbapenem resistance in clinical isolates of A. baumannii. METHODS: A. baumannii were isolated from various clinical specimens and identified based on Gram staining, biochemical tests, and PCR amplification of organism specific 16S rRNA and blaOXA-51 genes...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28131738/occurrence-of-blandm-7-within-incx3-type-plasmid-of-escherichia-coli-from-india
#11
Deepjyoti Paul, Amitabha Bhattacharjee, Birson Ingti, Nargis Alom Choudhury, Anand Prakash Maurya, Debadatta Dhar, Atanu Chakravarty
BACKGROUND: New-Delhi metallo-β-lactamase-7 with higher hydrolytic activity than its ancestor NDM-1 is emerging across the globe including India. In this study, we have investigated the genetic context of blaNDM-7 and alteration in plasmid copy number under concentration gradient carbapenem stress. MATERIALS AND METHODS: Six blaNDM-7 producing Escherichia coli isolates were obtained from Silchar Medical College and Hospital and the co-existence of other β-lactamases and transferability of this resistant determinant was determined by transformation and conjugation assay followed by typing of the plasmid by PBRT method...
April 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/27796645/spread-and-exchange-of-bla-ndm-1-in-hospitalized-neonates-role-of-mobilizable-genetic-elements
#12
S Datta, S Mitra, P Chattopadhyay, T Som, S Mukherjee, S Basu
To investigate the mobilizable elements associated with bla NDM-1 in Enterobacteriaceae isolated from septicaemic neonates at a NICU in India, during December, 2008-2011. An attempt was also made to understand whether there was a pattern in the temporal acquisition of bla NDM-1 within the unit. Transferability of carbapenem resistance was tested by conjugation and transformation. Plasmid types and addiction systems were analysed. The genetic background of bla NDM-1 and association with class 1 integron were evaluated by PCR mapping...
February 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27776788/diversity-of-polymyxin-resistance-mechanisms-among-acinetobacter-baumannii-clinical-isolates
#13
Raquel Girardello, Marina Visconde, Rodrigo Cayô, Regina Célia Bressan Queiroz de Figueiredo, Marcelo Alves da Silva Mori, Nilton Lincopan, Ana Cristina Gales
Polymyxins have become drugs of last resort for treatment of multi-drug resistant (MDR) Gram-negative infections. However, the mechanisms of resistance to this compound have not been completely elucidated. In this study, we evaluated the mechanisms of resistance to this antimicrobial in two A. baumannii clinical isolates, respectively, susceptible (A027) and resistant (A009) to polymyxin B before and after polymyxin B exposure (A027(ind) and A009(ind)). The pmrAB and lpxACD were sequenced and their transcriptional levels were analyzed by qRT-PCR...
January 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27551277/carbapenem-resistance-in-acinetobacter-baumannii-and-other-acinetobacter-spp-causing-neonatal-sepsis-focus-on-ndm-1-and-its-linkage-to-isaba125
#14
Somdatta Chatterjee, Saswati Datta, Subhasree Roy, Lavanya Ramanan, Anindya Saha, Rajlakshmi Viswanathan, Tapas Som, Sulagna Basu
Carbapenem-resistant determinants and their surrounding genetic structure were studied in Acinetobacter spp. from neonatal sepsis cases collected over 7 years at a tertiary care hospital. Acinetobacter spp. (n = 68) were identified by ARDRA followed by susceptibility tests. Oxacillinases, metallo-β-lactamases (MBLs), extended-spectrum β-lactamases and AmpCs, were detected phenotypically and/or by PCR followed by DNA sequencing. Transconjugants possessing the bla NDM-1(New Delhi metallo-β-lactamase) underwent further analysis for plasmids, integrons and associated genes...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27230482/k19-capsular-polysaccharide-of-acinetobacter-baumannii-is-produced-via-a-wzy-polymerase-encoded-in-a-small-genomic-island-rather-than-the-kl19-capsule-gene-cluster
#15
Johanna J Kenyon, Mikhail M Shneider, Sofya N Senchenkova, Alexander S Shashkov, Maria N Siniagina, Sergey Y Malanin, Anastasiya V Popova, Konstantin A Miroshnikov, Ruth M Hall, Yuriy A Knirel
Polymerization of the oligosaccharides (K units) of complex capsular polysaccharides (CPSs) requires a Wzy polymerase, which is usually encoded in the gene cluster that directs K unit synthesis. Here, a gene cluster at the Acinetobacter K locus (KL) that lacks a wzy gene, KL19, was found in Acinetobacter baumannii ST111 isolates 28 and RBH2 recovered from hospitals in the Russian Federation and Australia, respectively. However, these isolates produced long-chain capsule, and a wzy gene was found in a 6.1 kb genomic island (GI) located adjacent to the cpn60 gene...
August 2016: Microbiology
https://www.readbyqxmd.com/read/27154587/occurrence-of-co-existing-bla-vim-2-and-bla-ndm-1-in-clinical-isolates-of-pseudomonas-aeruginosa-from-india
#16
Deepjyoti Paul, Debadatta Dhar, Anand Prakash Maurya, Shweta Mishra, Gauri Dutt Sharma, Atanu Chakravarty, Amitabha Bhattacharjee
BACKGROUND: bla VIM-2 harboring Pseudomonas aeruginosa has been reported worldwide and considered as the most prevalent metallo-β-lactamase after NDM which are found horizontally transferable and mostly associated with integron gene cassettes. The present study investigates the genetic background, transmission dynamics as well as stability of bla VIM-2 in clinical isolates of P. aeruginosa harbor bla NDM-1 as well which were collected from October 2012 to September 2013. METHODS: Two P...
May 6, 2016: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/27065982/further-spread-of-bla-ndm-5-in-enterobacteriaceae-via-incx3-plasmids-in-shanghai-china
#17
Fangfang Zhang, Lianyan Xie, Xiaoli Wang, Lizhong Han, Xiaokui Guo, Yuxing Ni, Hongping Qu, Jingyong Sun
One hundred and two carbapenem-resistant Enterobacteriaceae (CRE) strains were isolated in a teaching hospital in Shanghai, China from 2012 to 2015. In a follow-up study, four New Delhi metallo-β-lactamase-5 (NDM-5)-producing strains were identified after screening these CRE strains, including 1 Klebsiella pneumoniae strain (RJ01), 1 Proteus mirabilis strain (RJ02), and 2 Escherichia coli strains (RJ03 and RJ04). All K. pneumoniae and E. coli isolates were resistant to carbapenems, third-generation cephalosporins, and piperacillin-tazobactam, but were susceptible to amikacin...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/26824943/novel-aminoglycoside-resistance-transposons-and-transposon-derived-circular-forms-detected-in-carbapenem-resistant-acinetobacter-baumannii-clinical-isolates
#18
Nabil Karah, Chinmay Kumar Dwibedi, Karin Sjöström, Petra Edquist, Anders Johansson, Sun Nyunt Wai, Bernt Eric Uhlin
Acinetobacter baumannii has emerged as an important opportunistic pathogen equipped with a growing number of antibiotic resistance genes. Our study investigated the molecular epidemiology and antibiotic resistance features of 28 consecutive carbapenem-resistant clinical isolates of A. baumannii collected throughout Sweden in 2012 and 2013. The isolates mainly belonged to clonal complexes (CCs) with an extensive international distribution, such as CC2 (n = 16) and CC25 (n = 7). Resistance to carbapenems was related to blaOXA-23 (20 isolates), blaOXA-24/40-like (6 isolates), blaOXA-467 (1 isolate), and ISAba1-blaOXA-69 (1 isolate)...
January 11, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26441902/attributes-of-carbapenemase-encoding-conjugative-plasmid-pndm-sal-from-an-extensively-drug-resistant-salmonella-enterica-serovar-senftenberg
#19
Anirban Sarkar, Gururaja P Pazhani, Goutam Chowdhury, Amit Ghosh, Thandavarayan Ramamurthy
A carbapenem resistant Salmonella enterica serovar Senftenberg isolate BCH 2406 was isolated from a diarrheal child attending an outpatient unit of B.C. Roy Hospital in Kolkata, India. This isolate was positive for the bla NDM-1 in the PCR assay, which was confirmed by amplicon sequencing. Except for tetracycline, this isolate was resistant to all the tested antimicrobials. The bla NDM-1 was found to be located on a 146.13-kb mega plasmid pNDM-SAL, which could be conjugally transferred into Escherichia coli and other enteric pathogens such as Vibrio cholerae O1 Ogawa and Shigella flexneri 2a...
2015: Frontiers in Microbiology
https://www.readbyqxmd.com/read/26308279/a-common-flanking-region-in-promiscuous-plasmids-encoding-blandm-1-in-klebsiella-pneumoniae-isolated-in-singapore
#20
Ying-Tsong Chen, L Kristopher Siu, Yu-Kuo Tsai, Fu-Mei Lin, Tse Hsien Koh, Jiun-Han Chen
Bacteria encoding the New Delhi metallo-β-lactamase gene (blaNDM-1) are regarded as superbugs for their resistance to multiple antibiotics. Plasmids encoding blaNDM-1 have been observed to be spreading among gram-negative bacteria around the world. Previous studies have demonstrated that multiple modifications of blaNDM-1-harboring plasmids might contribute to the spread of the gene. In this study, we analyzed blaNDM-1-encoding plasmids from two Klebsiella pneumoniae isolates, DU7433 and DU1301, found to be unrelated by pulsed field gel electrophoresis and multilocus sequencing typing (DU7433: ST14 and DU1301: ST11), and compared them with previously published plasmids...
March 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
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