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https://www.readbyqxmd.com/read/29109700/exploring-the-genome-and-phenotype-of-multi-drug-resistant-klebsiella-pneumoniae-of-clinical-origin
#1
João Anes, Daniel Hurley, Marta Martins, Séamus Fanning
Klebsiella pneumoniae is an important nosocomial pathogen with an extraordinary resistant phenotype due to a combination of acquired resistant-elements and efflux mechanisms. In this study a detailed molecular characterization of 11 K. pneumoniae isolates of clinical origin was carried out. Eleven clinical isolates were tested for their susceptibilities, by disk diffusion and broth microdilution and interpreted according to CLSI guidelines. Efflux activity was determined by measuring the extrusion of ethidium bromide and biofilm formation was assessed following static growth in Müeller-Hinton and minimal media M9 broths at two temperatures and time points...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29017452/whole-genome-sequencing-for-the-molecular-characterization-of-carbapenem-resistant-klebsiella-pneumoniae-strains-isolated-at-the-italian-asst-fatebenefratelli-sacco-hospital-2012-2014
#2
Sara Giordana Rimoldi, Bernardina Gentile, Cristina Pagani, Annamaria Di Gregorio, Anna Anselmo, Anna Maria Palozzi, Antonella Fortunato, Valentina Pittiglio, Anna Lisa Ridolfo, Maria Rita Gismondo, Giuliano Rizzardini, Florigio Lista
BACKGROUND: The emergence of carbapenem-resistant Klebsiella pneumoniae strains is threatening antimicrobial treatment. METHODS: Sixty-eight carbapenemase-producing K. pneumoniae strains isolated at Luigi Sacco University Hospital-ASST Fatebenefratelli Sacco (Milan, Italy) between 2012 and 2014 were characterised microbiologically and molecularly. They were tested for drug susceptibility and carbapenemase phenotypes, investigated by means of repetitive extra-genic palindromic polymerase chain reaction (REP-PCR), and fully sequenced by means of next-generation sequencing for the in silico analysis of multi-locus sequence typing (MLST), their resistome, virulome and plasmid content, and their core single nucleotide polymorphism (SNP) genotypes...
October 10, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28910381/genetic-characterization-of-blandm-harboring-plasmids-in-carbapenem-resistant-escherichia-coli-from-myanmar
#3
COMPARATIVE STUDY
Yo Sugawara, Yukihiro Akeda, Noriko Sakamoto, Dan Takeuchi, Daisuke Motooka, Shota Nakamura, Hideharu Hagiya, Norihisa Yamamoto, Isao Nishi, Hisao Yoshida, Kazuhisa Okada, Khwar Nyo Zin, Mya Mya Aye, Kazunori Tonomo, Shigeyuki Hamada
The bacterial enzyme New Delhi metallo-β-lactamase hydrolyzes almost all β-lactam antibiotics, including carbapenems, which are drugs of last resort for severe bacterial infections. The spread of carbapenem-resistant Enterobacteriaceae that carry the New Delhi metallo-β-lactamase gene, blaNDM, poses a serious threat to public health. In this study, we genetically characterized eight carbapenem-resistant Escherichia coli isolates from a tertiary care hospital in Yangon, Myanmar. The eight isolates belonged to five multilocus-sequence types and harbored multiple antimicrobial-resistance genes, resulting in resistance against nearly all of the antimicrobial agents tested, except colistin and fosfomycin...
2017: PloS One
https://www.readbyqxmd.com/read/28893788/genomic-and-molecular-characterization-of-clinical-isolates-of-enterobacteriaceae-harboring-mcr-1-in-colombia-2002-2016
#4
Sandra Yamile Saavedra, Lorena Diaz, Magdalena Wiesner, Adriana Correa, Stefany Alejandra Arévalo, Jinnethe Reyes, Andrea Melissa Hidalgo, Elsa de la Cadena, Marcela Perenguez, Lucy Angeline Montaño, Javier Ardila, Rafael Ríos, María Victoria Ovalle, Paula Díaz, Paola Porras, Maria V Villegas, Cesar A Arias, Mauricio Beltrán, Carolina Duarte
Polymyxins are last resort antimicrobial agents used to treat infections caused by carbapenem-resistant Enterobacteriaceae Due to the worldwide dissemination of polymyxin resistance in animal and human isolates, we aimed to characterize polymyxin resistance associated with the presence of mcr-1 in Enterobacteriaceae and nonfermenter Gram-negative bacilli, in isolates collected retrospectively in Colombia from 2002 to 2016. A total of 5,887 Gram-negative clinical isolates were studied and 513 were found to be resistant to the polymyxins...
September 11, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28893688/whole-genome-sequencing-identification-of-a-multidrug-resistant-salmonella-enterica-serovar-typhimurium-strain-carrying-blandm-5-from-guangdong-china
#5
Xiaoyan Li, Yan Jiang, Kuihai Wu, Yong Zhou, Ruike Liu, Yan Cao, Aiwu Wu, Yurong Qiu
A carbapenem-resistant Salmonella enterica serovar Typhimurium (sequence type 34 [ST34]) strain was isolated from a fecal specimen from a child with acute diarrhea. Whole-genome sequencing revealed that the 84.5-kb IncFII plasmid pST41-NDM carrying the NDM-5 carbapenemase gene possesses a structure identical to that of the IncFII-type plasmid backbone. However, the blaNDM-5 flanking sequence found in this plasmid is identical to the blaNDM-5-positive IncX3 plasmids carried by 10 strains of Enterobacteriaceae identified in the same hospital...
September 8, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28854680/the-ctx-m-14-plasmid-phk01-encodes-novel-small-rnas-and-influences-host-growth-and-motility
#6
Xinlei Jiang, Xuan Liu, Carmen O K Law, Ya Wang, Wai U Lo, Xing Weng, Ting Fung Chan, P L Ho, Terrence C K Lau
The dissemination of extended-spectrum β-lactamases (ESBLs) genes among bacteria is commonly achieved by plasmid conjugation. In the last decade, the CTX-M type enzyme was the most widespread and prevalent ESBLs in the world. In Hong Kong and mainland China, among the commonly found CTX-M-carrying plasmids were pHK01 and pHK01-like plasmids, which belong to incompatibility group FII (IncFII). In this work, we studied the physiological effect caused by the pHK01 plasmid in bacterial host Escherichia coli J53...
July 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28746989/instability-of-the-incfii-type-plasmid-carrying-blandm-5-in-a-klebsiella-pneumoniae-isolate
#7
Juyoun Shin, Jin Yang Baek, Doo Ryeon Chung, Kwan Soo Ko
In this study, we characterized the blaNDM-5-bearing plasmid in a Klebsiella pneumoniae isolate that had lost the plasmid during serial passage. We determined the complete sequences of the plasmid pCC1410-2, which was extracted from a K. pneumoniae ST709 isolate collected at a Korean hospital from which two NDM-5-producing K. pneumoniae isolates were subsequently isolated. As a result, the pCC1410-2 plasmid had a backbone structure that was similar to those of two plasmids previously reported from the same hospital, but lacked some antibiotic resistance genes (blaTEM-1, rmtB, mphR(A), mrx(A), and mph(A))...
September 28, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28725038/genomic-characterization-of-novel-incfii-type-multidrug-resistant-plasmids-p0716-kpc-and-p12181-kpc-from-klebsiella-pneumoniae
#8
Jiao Feng, Zhe Yin, Qiangyuan Zhao, Yachao Zhao, Defu Zhang, Xiaoyuan Jiang, Weili Wu, Weijun Chen, Hui Wang, Yajun Song, Yigang Tong, Jinglin Wang, Yanjun Li, Dongsheng Zhou
This study aimed to genetically characterize two fully-sequenced novel IncFII-type multidrug resistant (MDR) plasmids, p0716-KPC and p12181-KPC, recovered from two different clinical Klebsiella pneumoniae isolates. p0716-KPC and p12181-KPC had a very similar genomic content. The backbones of p0716-KPC/p12181-KPC contained two different replicons (belonging to a novel IncFII subtype and the Rep_3 family), the IncFIIK and IncFIIY maintenance regions, and conjugal transfer gene sets from IncFIIK-type plasmids and unknown origins...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28688673/characterization-of-phebe7-an-incfii-type-virulence-resistance-plasmid-carrying-blactx-m-98b-blatem-1-and-rmtb-genes-detected-in-escherichia-coli-from-a-chicken-isolate-in-china
#9
Yan-Xian Yang, Yong-Qiang Yang, Chang-Wei Lei, Bi-Hui Liu, Wei Jiang, Hong-Ning Wang, Maria Consuelo Gazitúa, An-Yun Zhang
Recently, a novel variant of the CTX-M enzyme, CTX-M-98, was detected in Escherichia coli isolates from food animals. However, few plasmids carrying blaCTX-M-98 have been fully characterized. In this study, we sequenced the complete pHeBE7 plasmid, an 86,015-bp plasmid that contains the blaCTX-M-98b, blaTEM-1, rmtB, and traT genes, using whole-genome sequencing. The backbone of pHeBE7 shows a high similarity (>99%) to pMC-NDM, which carries the blaNDM-1 gene, however its mosaic regions remain relatively unique among sequenced plasmids...
July 6, 2017: Plasmid
https://www.readbyqxmd.com/read/28642846/decreased-fitness-and-virulence-in-st10-escherichia-coli-harboring-blandm-5-and-mcr-1-against-a-st4981-strain-with-blandm-5
#10
Yawei Zhang, Kang Liao, Hua Gao, Qi Wang, Xiaojuan Wang, Henan Li, Ruobing Wang, Hui Wang
Although coexistence of blaNDM-5 and mcr-1 in Escherichia coli has been reported, little is known about the fitness and virulence of such strains. Three carbapenem-resistant Escherichia coli (GZ1, GZ2, and GZ3) successively isolated from one patient in 2015 were investigated for microbiological fitness and virulence. GZ1 and GZ2 were also resistant to colistin. To verify the association between plasmids and fitness, growth kinetics of the transconjugants were performed. We also analyzed genomic sequences of GZ2 and GZ3 using PacBio sequencing...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28498924/major-role-of-pkpqil-like-plasmids-in-the-early-dissemination-of-kpc-type-carbapenemases-in-the-uk
#11
M Doumith, J Findlay, H Hirani, K L Hopkins, D M Livermore, A Dodgson, N Woodford
Objectives: Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae were first seen in the UK in 2003 and have been increasingly reported since 2010, largely owing to an ongoing outbreak in North-West England. We examined the role of clonal spread and plasmid transmission in their emergence. Methods: Isolates comprised KPC-positive K. pneumoniae ( n  =   33), Escherichia coli ( n  =   7) and Enterobacter spp. ( n  =   4) referred to the national reference laboratory between 2008 and 2010 from 17 UK centres, including three in North-West England...
August 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28461314/ndm-5-and-oxa-181-beta-lactamases-a-significant-threat-continues-to-spread-in-the-americas
#12
Laura J Rojas, Andrea M Hujer, Susan D Rudin, Meredith S Wright, T Nicholas Domitrovic, Steven H Marshall, Kristine M Hujer, Sandra S Richter, Eric Cober, Federico Perez, Mark D Adams, David van Duin, Robert A Bonomo
Among Gram-negative bacteria, carbapenem-resistant infections pose a serious and life-threatening challenge. Here, the CRACKLE network reports a sentinel detection and characterization of a carbapenem-resistant Klebsiella pneumoniae ST147 isolate harboring blaNDM-5 and blaOXA-181 from a young man who underwent abdominal surgery in India. blaNDM-5 was located on an IncFII plasmid of ≈90 kb, whereas blaOXA-181 was chromosomally encoded. Resistome and genome analysis demonstrated multiple copies of the transposable element IS26 and a "hot-spot region" in the IncFII plasmid...
July 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28438945/multihospital-occurrence-of-pan-resistant-klebsiella-pneumoniae-sequence-type-147-with-an-isecp1-directed-blaoxa-181-insertion-in-the-mgrb-gene-in-the-united-arab-emirates
#13
Ágnes Sonnevend, Akela Ghazawi, Rayhan Hashmey, Aliasgher Haidermota, Safinaz Girgis, Mubarak Alfaresi, Mohammed Omar, David L Paterson, Hosam M Zowawi, Tibor Pál
The emergence of pan-resistant Klebsiella pneumoniae strains is an increasing concern. In the present study, we describe a cluster of 9 pan-resistant K. pneumoniae sequence type 147 (ST147) isolates encountered in 4 patients over nearly 1 year in 3 hospitals of the United Arab Emirates (UAE). The isolates exhibited highly similar genotypes. All produced chromosomally encoded OXA-181, and the majority also produced the NDM-5 carbapenemase. As with the previously described single isolate from the UAE, MS6671, the mgrB was disrupted by a functional, ISEcp1-driven blaOXA-181 insertion causing resistance to carbapenems...
July 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28405633/genomic-analysis-of-factors-associated-with-low-prevalence-of-antibiotic-resistance-in-extraintestinal-pathogenic-escherichia-coli-sequence-type-95-strains
#14
Craig M Stephens, Sheila Adams-Sapper, Manraj Sekhon, James R Johnson, Lee W Riley
Extraintestinal pathogenic Escherichia coli (ExPEC) strains belonging to multilocus sequence type 95 (ST95) are globally distributed and a common cause of infections in humans and domestic fowl. ST95 isolates generally show a lower prevalence of acquired antimicrobial resistance than other pandemic ExPEC lineages. We took a genomic approach to identify factors that may underlie reduced resistance. We fully assembled genomes for four ST95 isolates representing the four major fimH-based lineages within ST95 and also analyzed draft-level genomes from another 82 ST95 isolates, largely from the western United States...
March 2017: MSphere
https://www.readbyqxmd.com/read/28395597/genome-sequencing-and-comparative-analysis-of-an-ndm-1-producing-klebsiella-pneumoniae-st15-isolated-from-a-refugee-patient
#15
Tamara Salloum, Harout Arabaghian, Sahar Alousi, Edmond Abboud, Sima Tokajian
The escalating problem of antibiotic resistance, specifically cabarpenemase and extended-spectrum β-lacatamase (ESBL) producing K. pneumoniae strains, is directly correlated with increased patient morbidity and mortality and prolonged hospitalization and costs. In this study, a comprehensive genomic analysis encompassing the resistomics, virulence repertoire and mobile genetic elements of an NDM-1 positive ESBL-producing K. pneumoniae EA-MEH ST15 isolated from a urine sample collected from a Syrian refugee was conducted...
June 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/28379732/prevalence-of-plasmid-mediated-fosfomycin-resistance-gene-fosa3-among-ctx-m-producing-escherichia-coli-isolates-from-chickens-in-china
#16
Wei Jiang, Shuai Men, Linghan Kong, Suzhen Ma, Yongqiang Yang, Yongxiang Wang, Qiwu Yuan, Guangyang Cheng, Wencheng Zou, Hongning Wang
The aim of this study was to investigate the prevalence of fosfomycin resistance gene fosA3 and characterize plasmids harboring fosA3 among CTX-M-producing Escherichia coli from chickens in China. A total of 234 CTX-M-producing E. coli isolates collected from chickens from 2014 to 2016 were screened for the presence of plasmid-mediated fosfomycin resistance genes (fosA, fosA3, and fosC2). Clonal relatedness of fosA3-positive isolates was determined by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)...
April 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28223374/enterobacter-cloacae-complex-isolates-harboring-blanmc-a-or-blaimi-type-class-a-carbapenemase-genes-on-novel-chromosomal-integrative-elements-and-plasmids
#17
David A Boyd, Laura F Mataseje, Ross Davidson, Johannes A Delport, Jeff Fuller, Linda Hoang, Brigitte Lefebvre, Paul N Levett, Diane L Roscoe, Barbara M Willey, Michael R Mulvey
Carbapenem-resistant Enterobacter cloacae complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by PCR for seven common carbapenemase gene groups, namely, KPC, NDM, OXA-48, VIM, IMP, GES, and NMC-A/IMI. Nineteen of the submitted isolates (1.7%) were found to harbor Ambler class A blaNMC-A or blaIMI-type carbapenemases. All 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprofloxacin. Most isolates (17/19) gave positive results with the Carba-NP test for phenotypic carbapenemase detection...
May 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28185686/outbreak-of-kpc-2-producing-enterobacteriaceae-caused-by-clonal-dissemination-of-klebsiella-pneumoniae-st307-carrying-an-incx3-type-plasmid-harboring-a-truncated-tn4401a
#18
Jung Ok Kim, Sae Am Song, Eun-Jeong Yoon, Jeong Hwan Shin, Hyukmin Lee, Seok Hoon Jeong, Kyungwon Lee
Over a 5-month period between the end of June and the beginning of November in 2015, a KPC-producing Enterobacteriaceae outbreak occurred in a general hospital in Busan, South Korea, being associated with a total of 50 clinical isolates from 47 patients. Multilocus sequence typing and pulsed-field gel electrophoresis were carried out for strain typing and whole-genome sequencing was performed to characterize the plasmids. A clonal spread of K. pneumoniae sequence type 307 (ST307) carrying a self-transferable IncX3-type plasmid harboring blaKPC-2 was responsible for the outbreak...
April 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28087584/emergence-of-klebsiella-variicola-positive-for-ndm-9-a-variant-of-new-delhi-metallo-%C3%AE-lactamase-in-an-urban-river-in-south-korea
#19
Doris Y W Di, Jeonghwan Jang, Tatsuya Unno, Hor-Gil Hur
Objectives: To examine the presence of pathogenic bacteria carrying New Delhi metallo-β-lactamase in the environment and to characterize the genome structures of these strains. Methods: Phenotypic screening of antimicrobial susceptibility and WGS were conducted on three Klebsiella variicola strains possessing NDM-9 isolated from an urban river. Results: Three carbapenem-resistant K. variicola isolated from Gwangju tributary were found to possess bla NDM-9 genes...
April 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27938421/high-prevalence-of-fosfomycin-resistance-gene-fosa3-in-bla-ctx-m-harbouring-escherichia-coli-from-urine-in-a-chinese-tertiary-hospital-during-2010-2014
#20
X-L Cao, H Shen, Y-Y Xu, X-J Xu, Z-F Zhang, L Cheng, J-H Chen, Y Arakawa
Fosfomycin has become a therapeutic option in urinary tract infections. We identified 57 fosfomycin-resistant Escherichia coli from 465 urine-derived extended-spectrum β-lactamase (ESBL)-producing isolates from a Chinese hospital during 2010-2014. Of the 57 fosfomycin-resistant isolates, 51 (89·5%) carried fosA3, and one carried fosA1. Divergent pulsed-field gel electrophoresis profiles and multi-locus sequence typing results revealed high clonal diversity in the fosA3-positive isolates. Conjugation experiments showed that the fosA3 genes from 50 isolates were transferable, with IncFII or IncI1 being the most prevalent types of plasmids...
March 2017: Epidemiology and Infection
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