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https://www.readbyqxmd.com/read/27919902/genetic-environment-of-the-blakpc-2-gene-in-klebsiella-pneumoniae-isolate-which-may-have-been-imported-to-russia-from-southeast-asia
#1
Vladimir Ageevets, Julia Sopova, Irina Lazareva, Maya Malakhova, Elena Ilina, Elena Kostryukova, Vladislav Babenko, Alessandra Carattoli, Yuri Lobzin, Alexander Uskov, Sergey Sidorenko
The nucleotide sequence of a blaKPC-2-harboring plasmid (pKPCAPSS) from Klebsiella pneumoniae ST273 isolated in Saint Petersburg, Russia from a patient with history of recent travel to Vietnam is presented. This 127,970-bp plasmid possessed both IncFII and IncR replicons. blaKPC-2 was localized on a hypothetical mobile element. This element was flanked by 38bp inverted Tn3 repeats and included a Tn3-specific transposase gene, macrolide resistance operon (mphA, mrx, mphR), and a fragment of blaTEM with unique polymorphisms...
December 5, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27833607/complete-sequence-of-a-f33-a-b-conjugative-plasmid-carrying-the-oqxab-fosa3-and-blactx-m-55-elements-from-a-foodborne-escherichia-coli-strain
#2
Marcus H Wong, Miaomiao Xie, Liqi Xie, Dachuan Lin, Ruichao Li, Yuanjie Zhou, Edward W Chan, Sheng Chen
This study reports the complete sequence of pE80, a conjugative IncFII plasmid recovered from an Escherichia coli strain isolated from chicken meat. This plasmid harbors multiple resistance determinants including oqxAB, fosA3, blaCTX-M-55, and blaTEM-1, and is a close variant of the recently reported p42-2 element, which was recovered from E. coli of veterinary source. Recovery of pE80 constitutes evidence that evolution or genetic re-arrangement of IncFII type plasmids residing in animal-borne organisms is an active event, which involves acquisition and integration of foreign resistance elements into the plasmid backbone...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27821455/incfii-conjugative-plasmid-mediated-transmission-of-blandm-1-elements-among-animal-borne-e-coli-strains
#3
Dachuan Lin, Miaomiao Xie, Ruichao Li, Kaichao Chen, Edward Wai-Chi Chan, Sheng Chen
This study aims to investigate the prevalence and transmission dynamics blaNDM-1 gene in animal E coli strains. Two IncFII blaNDM-1-encodingplasmids, pHNEC46-NDM and pHNEC55-NDM, with only minor structural variation in the MDR region were found to be responsible for the transmission of blaNDM-1 in these strains. The blaNDM-1 gene can be incorporated into plasmids and stably inherited in animal-borne E. coli strains that can be maintained in animal gut microflora even without carbapenem selection pressure.
November 7, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27796645/spread-and-exchange-of-bla-ndm-1-in-hospitalized-neonates-role-of-mobilizable-genetic-elements
#4
S Datta, S Mitra, P Chattopadhyay, T Som, S Mukherjee, S Basu
To investigate the mobilizable elements associated with bla NDM-1 in Enterobacteriaceae isolated from septicaemic neonates at a NICU in India, during December, 2008-2011. An attempt was also made to understand whether there was a pattern in the temporal acquisition of bla NDM-1 within the unit. Transferability of carbapenem resistance was tested by conjugation and transformation. Plasmid types and addiction systems were analysed. The genetic background of bla NDM-1 and association with class 1 integron were evaluated by PCR mapping...
October 28, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27766094/the-first-report-of-a-fully-sequenced-resistance-plasmid-from-shigella-boydii
#5
Li Wang, Lei Liu, Dong Liu, Zhe Yin, Jiao Feng, Defu Zhang, Haihong Fang, Yefeng Qiu, Weijun Chen, Ruisheng Yang, Jinglin Wang, Yunzhi Fa, Dongsheng Zhou
The purpose of this study was to characterize mechanisms of plasmid-mediated antimicrobial resistance in Shigella boydii. S. boydii strain 2246 with resistance to ciprofloxacin, ceftriaxone and azithromycin was isolated from a human case of watery diarrhea in a Chinese public hospital. Resistance in strain 2246 to ceftriaxone and azithromycin was attributable to the presence of blaCTX-M-14, and erm(B) and mph(A), respectively, which were co-located on a multidrug-resistant (MDR) plasmid p2246-CTXM. p2246-CTXM represented a novel IncFII-type MDR plasmid with a very complex chimera structure...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27671066/whole-genome-sequencing-identifies-in-vivo-acquisition-of-a-blactx-m-27-carrying-incfii-transmissible-plasmid-as-the-cause-of-ceftriaxone-treatment-failure-for-an-invasive-salmonella-enterica-serovar-typhimurium-infection
#6
Bruce McCollister, Cassandra V Kotter, Daniel N Frank, Taylor Washburn, Michael G Jobling
We report a case of ceftriaxone treatment failure for bacteremia caused by Salmonella enterica subsp. enterica serovar Typhimurium, due to the in vivo acquisition of a blaCTX-M-27-encoding IncFII group transmissible plasmid. The original β-lactamase-susceptible isolate ST882S was replaced by the resistant isolate ST931R during ceftriaxone treatment. After relapse, treatment was changed to ciprofloxacin, and the patient recovered. Isolate ST931R could transfer resistance to Escherichia coli at 37°C. We used whole-genome sequencing of ST882S and ST931R, the E...
December 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27527778/absence-of-co-localization-between-pathovar-associated-virulence-factors-and-extended-spectrum-%C3%AE-lactamase-blactx-m-genes-on-a-single-plasmid
#7
Charlotte Valat, Karine Forest, Méganne Billet, Charlène Polizzi, Estelle Saras, Jean-Yves Madec, Marisa Haenni
Extended-spectrum β-lactamases (ESBLs) were reported in virulent food-borne Escherichia coli clones, and numerous genes encoding ESBLs and virulence factors (VFs) are plasmid-mediated. We investigated the plasmidic co-localization of ESBL genes and pathovar-associated VF genes isolated in 18 E. coli isolates from faecal samples of diseased cattle. From the rare ESBL-producing E. coli among the various pathovars, no plasmid co-localization was found between VF and blaCTX-M genes on a single plasmid. However, a link between replicon types and VFs was highlighted: EspP was associated with IncFIB and ToxB with IncB/O...
August 30, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27365311/increased-plasmid-copy-number-is-essential-for-yersinia-t3ss-function-and-virulence
#8
He Wang, Kemal Avican, Anna Fahlgren, Saskia F Erttmann, Aaron M Nuss, Petra Dersch, Maria Fallman, Tomas Edgren, Hans Wolf-Watz
Pathogenic bacteria have evolved numerous virulence mechanisms that are essential for establishing infections. The enterobacterium Yersinia uses a type III secretion system (T3SS) encoded by a 70-kilobase, low-copy, IncFII-class virulence plasmid. We report a novel virulence strategy in Y. pseudotuberculosis in which this pathogen up-regulates the plasmid copy number during infection. We found that an increased dose of plasmid-encoded genes is indispensable for virulence and substantially elevates the expression and function of the T3SS...
July 29, 2016: Science
https://www.readbyqxmd.com/read/27363583/active-surveillance-scheme-in-three-romanian-hospitals-reveals-a-high-prevalence-and-variety-of-carbapenamase-producing-gram-negative-bacteria-a-pilot-study-december-2014-to-may-2015
#9
Dorina Timofte, Carmen Valentina Panzaru, Iuliana Elena Maciuca, Maria Dan, Anca Delia Mare, Adrian Man, Felicia Toma
We report the findings of an active surveillance scheme for detection of asymptomatic carriers with carbapenemase-producing Gram-negative bacteria (CP-GNB) in Romanian hospitals. During a pilot study from December 2014 to May 2015, faecal cultures were screened in three hospitals (two large, one medium-size) for patients newly admitted to selected wards or inpatients transferred from other wards to an intensive-care unit. The study revealed a high prevalence of CP-GNB detected in 22/27 and 28/38 of the carbapenem non-susceptible isolates from Hospitals 1 and 3, respectively...
June 23, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/27261267/glutathione-s-transferase-fosa6-of-klebsiella-pneumoniae-origin-conferring-fosfomycin-resistance-in-esbl-producing-escherichia-coli
#10
Qinglan Guo, Adam D Tomich, Christi L McElheny, Vaughn S Cooper, Nicole Stoesser, Minggui Wang, Nicolas Sluis-Cremer, Yohei Doi
OBJECTIVES: The objectives of this study were to elucidate the genetic context of a novel plasmid-mediated fosA variant, fosA6, conferring fosfomycin resistance and to characterize the kinetic properties of FosA6. METHODS: The genome of fosfomycin-resistant Escherichia coli strain YD786 was sequenced. Homologues of FosA6 were identified through BLAST searches. FosA6 and FosA(ST258) were purified and characterized using a steady-state kinetic approach. Inhibition of FosA activity was examined with sodium phosphonoformate...
September 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27183358/characterization-of-the-genetic-environment-of-the-blakpc-2-gene-among-klebsiella-pneumoniae-isolates-from-a-chinese-hospital
#11
Pinghua Shen, Ying Zhang, Gang Li, Xiaofei Jiang
Infection caused by carbapenem-resistant Klebsiella pneumoniae has become a major healthcare threat and KPC-2 enzyme is a dominant factor mediating carbapenems resistance in K. pneumoniae. This study was designed to determine the genetic environment of blaKPC-2, which prevailed in clinical K. pneumoniae isolates recovered in Huashan Hospital, Shanghai, China. Forty-two clinical isolates were included in this study by blaKPC-2 screening. After multilocus sequence typing and plasmid analyses of PCR-based replicon typing (PBRT), junction PCR, mapping PCR and crossing PCR assays, primer walking, and amplicon sequencing were used to analyze the genetic environment of the blaKPC-2 gene...
July 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27139028/persistent-spread-of-the-rmtb-16s-rrna-methyltransferase-gene-among-escherichia-coli-isolates-from-diseased-food-producing-animals-in-china
#12
Jing Xia, Jian Sun, Ke Cheng, Liang Li, Liang-Xing Fang, Meng-Ting Zou, Xiao-Ping Liao, Ya-Hong Liu
A total of 963 non-duplicate Escherichia coli strains isolated from food-producing animals between 2002 and 2012 were screened for the presence of the 16S rRNA methyltransferase genes. Among the positive isolates, resistance determinants to extended spectrum β-lactamases, plasmid-mediated quinolone resistance genes as well as floR and fosA/A3/C2 were detected using PCR analysis. These isolates were further subjected to antimicrobial susceptibility testing, molecular typing, PCR-based plasmid replicon typing and plasmid analysis...
May 30, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27067314/complete-sequence-of-the-fii-plasmid-p42-2-carrying-blactx-m-55-oqxab-fosa3-and-flor-from-escherichia-coli
#13
Qiu E Yang, Timothy Rutland Walsh, Bao Tao Liu, Meng Ting Zou, Hui Deng, Liang Xing Fang, Xiao Ping Liao, Jian Sun, Ya Hong Liu
We sequenced a novel conjugative multidrug resistance IncF plasmid, p42-2, isolated from Escherichia coli strain 42-2, previously identified in China. p42-2 is 106,886 bp long, composed of a typical IncFII-type backbone (∼54 kb) and one distinct acquired DNA region spanning ∼53 kb, harboring 12 antibiotic resistance genes [blaCTX-M-55, oqxA, oqxB, fosA3, floR, tetA(A), tetA(R), strA, strB, sul2, aph(3')-II, and ΔblaTEM-1]. The spread of these multidrug resistance determinants on the same plasmid is of great concern and, because of coresistance to antibiotics from different classes, is therapeutically challenging...
July 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27006459/evolutionary-history-of-the-global-emergence-of-the-escherichia-coli-epidemic-clone-st131
#14
Nicole Stoesser, Anna E Sheppard, Louise Pankhurst, Nicola De Maio, Catrin E Moore, Robert Sebra, Paul Turner, Luke W Anson, Andrew Kasarskis, Elizabeth M Batty, Veronica Kos, Daniel J Wilson, Rattanaphone Phetsouvanh, David Wyllie, Evgeni Sokurenko, Amee R Manges, Timothy J Johnson, Lance B Price, Timothy E A Peto, James R Johnson, Xavier Didelot, A Sarah Walker, Derrick W Crook
UNLABELLED: Escherichia colisequence type 131 (ST131) has emerged globally as the most predominant extraintestinal pathogenic lineage within this clinically important species, and its association with fluoroquinolone and extended-spectrum cephalosporin resistance impacts significantly on treatment. The evolutionary histories of this lineage, and of important antimicrobial resistance elements within it, remain unclearly defined. This study of the largest worldwide collection (n= 215) of sequenced ST131E...
March 22, 2016: MBio
https://www.readbyqxmd.com/read/26888909/atypical-epidemiology-of-ctx-m-15-among-enterobacteriaceae-from-a-high-diversity-of-non-clinical-niches-in-angola
#15
T G Ribeiro, Â Novais, L Peixe, E Machado
OBJECTIVES: The objective of this study was to investigate the distribution and molecular epidemiology of ESBLs, acquired AmpCs and carbapenemases in Enterobacteriaceae from non-clinical niches in Angola, an under-researched sub-Saharan country. METHODS: Eighty-one samples were recovered from healthy persons (n = 18), healthy animals (n = 33) and their environments (n = 10) or aquatic settings (n = 20) in south Angola (2013). Samples were plated onto CHROMagar™ Orientation with/without antibiotics...
May 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/26856843/molecular-characterization-of-escherichia-coli-strains-isolated-from-retail-meat-that-harbor-blactx-m-and-fosa3-genes
#16
Miaomiao Xie, Dachuan Lin, Kaichao Chen, Edward Wai Chi Chan, Wen Yao, Sheng Chen
A total of 55 cefotaxime-resistantEscherichia coliisolates were obtained from retail meat products purchased in Shenzhen, China, during the period November 2012 to May 2013. Thirty-seven of these 55 isolates were found to harbor ablaCTX-Mgene, with theblaCTX-M-1group being the most common type.blaCMY-2was detected in 16 isolates, alone or in combination with other extended-spectrum β-lactamase (ESBL) determinants. Importantly, thefosA3gene, which encodes fosfomycin resistance, was detected in 12 isolates, with several being found to reside in the conjugative plasmid that harbored theblaCTX-Mgene...
April 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26824953/blandm-5-bearing-incfii-type-plasmids-of-klebsiella-pneumoniae-sequence-type-147-transmitted-by-cross-border-transfer-of-a-patient
#17
Juyoun Shin, Jin Yang Baek, Sun Young Cho, Hee Jae Huh, Nam Yong Lee, Jae-Hoon Song, Doo Ryeon Chung, Kwan Soo Ko
The two plasmids extracted from Klebsiella pneumoniae sequence type 147 (ST147) isolates were analyzed. The first isolate was obtained from a patient transferred from United Arab Emirates to South Korea. The second isolate was obtained from a Korean patient and was suspected to be transmitted from the first patient. Sequences of two plasmids were almost the same, and genetic structures, including blaNDM-5, of these plasmids were similar to plasmids of NDM-1-producing Escherichia coli ST131 isolates found in Europe...
March 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26812051/tanzawaic-acids-a-chemically-novel-set-of-bacterial-conjugation-inhibitors
#18
María Getino, Raúl Fernández-López, Carolina Palencia-Gándara, Javier Campos-Gómez, Jose M Sánchez-López, Marta Martínez, Antonio Fernández, Fernando de la Cruz
Bacterial conjugation is the main mechanism for the dissemination of multiple antibiotic resistance in human pathogens. This dissemination could be controlled by molecules that interfere with the conjugation process. A search for conjugation inhibitors among a collection of 1,632 natural compounds, identified tanzawaic acids A and B as best hits. They specially inhibited IncW and IncFII conjugative systems, including plasmids mobilized by them. Plasmids belonging to IncFI, IncI, IncL/M, IncX and IncH incompatibility groups were targeted to a lesser extent, whereas IncN and IncP plasmids were unaffected...
2016: PloS One
https://www.readbyqxmd.com/read/26552763/molecular-epidemiology-of-carbapenem-resistant-enterobacteriaceae-in-valle-d-aosta-region-italy-shows-the-emergence-of-kpc-2-producing-klebsiella-pneumoniae-clonal-complex-101-st101-and-st1789
#19
Mariateresa Del Franco, Laura Paone, Roberto Novati, Claudio G Giacomazzi, Maria Bagattini, Chiara Galotto, Pier Giorgio Montanera, Maria Triassi, Raffaele Zarrilli
BACKGROUND: The spread of carbapenem resistant Enterobacteriaceae (CRE) is an emerging clinical problem, of great relevance in Europe and worldwide. The aim of this study was the molecular epidemiology of CRE isolates in Valle d'Aosta region, Italy, and the mechanism of carbapenem resistance. RESULTS: Sixty consecutive CRE samples were isolated from 52 hospital inpatients and/or outpatients from November 2013 to August 2014. Genotyping of microbial isolates was done by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST), carbapenemases were identified by PCR and sequencing...
2015: BMC Microbiology
https://www.readbyqxmd.com/read/26488485/fosfomycin-resistance-in-escherichia-coli-pennsylvania-usa
#20
Hind Alrowais, Christi L McElheny, Caressa N Spychala, Sangeeta Sastry, Qinglan Guo, Adeel A Butt, Yohei Doi
Fosfomycin resistance in Escherichia coli is rare in the United States. An extended-spectrum β-lactamase-producing E. coli clinical strain identified in Pennsylvania, USA, showed high-level fosfomycin resistance caused by the fosA3 gene. The IncFII plasmid carrying this gene had a structure similar to those found in China, where fosfomycin resistance is commonly described.
November 2015: Emerging Infectious Diseases
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