keyword
MENU ▼
Read by QxMD icon Read
search

IncFII

keyword
https://www.readbyqxmd.com/read/28223374/enterobacter-cloacae-complex-isolates-harboring-blanmc-a-or-blaimi-type-class-a-carbapenemase-genes-on-novel-chromosomal-integrative-elements-and-plasmids
#1
David A Boyd, Laura F Mataseje, Ross Davidson, Johannes A Delport, Jeff Fuller, Linda Hoang, Brigitte Lefebvre, Paul Levett, Diane L Roscoe, Barbara M Willey, Michael R Mulvey
Carbapenem-resistant Enterobacter cloacae complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by PCR for seven common carbapenemase gene groups, namely, KPC, NDM, OXA-48, VIM, IMP, GES, and NMC-A/IMI. Nineteen of the submitted isolates (1.7%) were found to harbor Ambler class A blaNMC-A or blaIMI-type carbapenemases. All 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprofloxacin. Most isolates (17/19) gave positive results with the Carba-NP test for phenotypic carbapenemase detection...
February 21, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28185686/outbreak-of-kpc-2-producing-enterobacteriaceae-caused-by-clonal-dissemination-of-klebsiella-pneumoniae-st307-carrying-an-incx3-type-plasmid-harboring-a-truncated-tn4401a
#2
Jung Ok Kim, Sae Am Song, Eun-Jeong Yoon, Jeong Hwan Shin, Hyukmin Lee, Seok Hoon Jeong, Kyungwon Lee
Over a 5-month period between the end of June and the beginning of November in 2015, a KPC-producing Enterobacteriaceae outbreak occurred in a general hospital in Busan, South Korea, being associated with a total of 50 clinical isolates from 47 patients. Multilocus sequence typing and pulsed-field gel electrophoresis were carried out for strain typing and whole-genome sequencing was performed to characterize the plasmids. A clonal spread of K. pneumoniae sequence type 307 (ST307) carrying a self-transferable IncX3-type plasmid harboring blaKPC-2 was responsible for the outbreak...
December 27, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28087584/emergence-of-klebsiella-variicola-positive-for-ndm-9-a-variant-of-new-delhi-metallo-%C3%AE-lactamase-in-an-urban-river-in-south-korea
#3
Doris Y W Di, Jeonghwan Jang, Tatsuya Unno, Hor-Gil Hur
OBJECTIVES: To examine the presence of pathogenic bacteria carrying New Delhi metallo-β-lactamase in the environment and to characterize the genome structures of these strains. METHODS: Phenotypic screening of antimicrobial susceptibility and WGS were conducted on three Klebsiella variicola strains possessing NDM-9 isolated from an urban river. RESULTS: Three carbapenem-resistant K. variicola isolated from Gwangju tributary were found to possess blaNDM-9 genes...
January 12, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27938421/high-prevalence-of-fosfomycin-resistance-gene-fosa3-in-bla-ctx-m-harbouring-escherichia-coli-from-urine-in-a-chinese-tertiary-hospital-during-2010-2014
#4
X-L Cao, H Shen, Y-Y Xu, X-J Xu, Z-F Zhang, L Cheng, J-H Chen, Y Arakawa
Fosfomycin has become a therapeutic option in urinary tract infections. We identified 57 fosfomycin-resistant Escherichia coli from 465 urine-derived extended-spectrum β-lactamase (ESBL)-producing isolates from a Chinese hospital during 2010-2014. Of the 57 fosfomycin-resistant isolates, 51 (89·5%) carried fosA3, and one carried fosA1. Divergent pulsed-field gel electrophoresis profiles and multi-locus sequence typing results revealed high clonal diversity in the fosA3-positive isolates. Conjugation experiments showed that the fosA3 genes from 50 isolates were transferable, with IncFII or IncI1 being the most prevalent types of plasmids...
December 12, 2016: Epidemiology and Infection
https://www.readbyqxmd.com/read/27919902/genetic-environment-of-the-blakpc-2-gene-in-a-klebsiella-pneumoniae-isolate-that-may-have-been-imported-to-russia-from-southeast-asia
#5
Vladimir Ageevets, Julia Sopova, Irina Lazareva, Maya Malakhova, Elena Ilina, Elena Kostryukova, Vladislav Babenko, Alessandra Carattoli, Yuri Lobzin, Alexander Uskov, Sergey Sidorenko
The nucleotide sequence of a blaKPC-2-harboring plasmid (pKPCAPSS) from Klebsiella pneumoniae ST273 isolated in Saint Petersburg, Russia, from a patient with history of recent travel to Vietnam is presented. This 127,970-bp plasmid possessed both IncFII and IncR replicons. blaKPC-2 was localized on a hypothetical mobile element. This element was flanked by 38-bp inverted Tn3 repeats and included a Tn3-specific transposase gene, macrolide resistance operon (mphA-mrx-mphR), and a fragment of blaTEM with unique polymorphisms...
February 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27833607/complete-sequence-of-a-f33-a-b-conjugative-plasmid-carrying-the-oqxab-fosa3-and-blactx-m-55-elements-from-a-foodborne-escherichia-coli-strain
#6
Marcus H Wong, Miaomiao Xie, Liqi Xie, Dachuan Lin, Ruichao Li, Yuanjie Zhou, Edward W Chan, Sheng Chen
This study reports the complete sequence of pE80, a conjugative IncFII plasmid recovered from an Escherichia coli strain isolated from chicken meat. This plasmid harbors multiple resistance determinants including oqxAB, fosA3, blaCTX-M-55, and blaTEM-1, and is a close variant of the recently reported p42-2 element, which was recovered from E. coli of veterinary source. Recovery of pE80 constitutes evidence that evolution or genetic re-arrangement of IncFII type plasmids residing in animal-borne organisms is an active event, which involves acquisition and integration of foreign resistance elements into the plasmid backbone...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27821455/incfii-conjugative-plasmid-mediated-transmission-of-blandm-1-elements-among-animal-borne-escherichia-coli-strains
#7
Dachuan Lin, Miaomiao Xie, Ruichao Li, Kaichao Chen, Edward Wai-Chi Chan, Sheng Chen
This study aims to investigate the prevalence and transmission dynamics of the blaNDM-1 gene in animal Escherichia coli strains. Two IncFII blaNDM-1-encoding plasmids with only minor structural variation in the MDR region, pHNEC46-NDM and pHNEC55-NDM, were found to be responsible for the transmission of blaNDM-1 in these strains. The blaNDM-1 gene can be incorporated into plasmids and stably inherited in animal-borne E. coli strains that can be maintained in animal gut microflora even without carbapenem selection pressure...
January 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27796645/spread-and-exchange-of-bla-ndm-1-in-hospitalized-neonates-role-of-mobilizable-genetic-elements
#8
S Datta, S Mitra, P Chattopadhyay, T Som, S Mukherjee, S Basu
To investigate the mobilizable elements associated with bla NDM-1 in Enterobacteriaceae isolated from septicaemic neonates at a NICU in India, during December, 2008-2011. An attempt was also made to understand whether there was a pattern in the temporal acquisition of bla NDM-1 within the unit. Transferability of carbapenem resistance was tested by conjugation and transformation. Plasmid types and addiction systems were analysed. The genetic background of bla NDM-1 and association with class 1 integron were evaluated by PCR mapping...
February 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27766094/the-first-report-of-a-fully-sequenced-resistance-plasmid-from-shigella-boydii
#9
Li Wang, Lei Liu, Dong Liu, Zhe Yin, Jiao Feng, Defu Zhang, Haihong Fang, Yefeng Qiu, Weijun Chen, Ruisheng Yang, Jinglin Wang, Yunzhi Fa, Dongsheng Zhou
The purpose of this study was to characterize mechanisms of plasmid-mediated antimicrobial resistance in Shigella boydii. S. boydii strain 2246 with resistance to ciprofloxacin, ceftriaxone and azithromycin was isolated from a human case of watery diarrhea in a Chinese public hospital. Resistance in strain 2246 to ceftriaxone and azithromycin was attributable to the presence of blaCTX-M-14, and erm(B) and mph(A), respectively, which were co-located on a multidrug-resistant (MDR) plasmid p2246-CTXM. p2246-CTXM represented a novel IncFII-type MDR plasmid with a very complex chimera structure...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27671066/whole-genome-sequencing-identifies-in-vivo-acquisition-of-a-blactx-m-27-carrying-incfii-transmissible-plasmid-as-the-cause-of-ceftriaxone-treatment-failure-for-an-invasive-salmonella-enterica-serovar-typhimurium-infection
#10
Bruce McCollister, Cassandra V Kotter, Daniel N Frank, Taylor Washburn, Michael G Jobling
We report a case of ceftriaxone treatment failure for bacteremia caused by Salmonella enterica subsp. enterica serovar Typhimurium, due to the in vivo acquisition of a blaCTX-M-27-encoding IncFII group transmissible plasmid. The original β-lactamase-susceptible isolate ST882S was replaced by the resistant isolate ST931R during ceftriaxone treatment. After relapse, treatment was changed to ciprofloxacin, and the patient recovered. Isolate ST931R could transfer resistance to Escherichia coli at 37°C. We used whole-genome sequencing of ST882S and ST931R, the E...
December 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27527778/absence-of-co-localization-between-pathovar-associated-virulence-factors-and-extended-spectrum-%C3%AE-lactamase-blactx-m-genes-on-a-single-plasmid
#11
Charlotte Valat, Karine Forest, Méganne Billet, Charlène Polizzi, Estelle Saras, Jean-Yves Madec, Marisa Haenni
Extended-spectrum β-lactamases (ESBLs) were reported in virulent food-borne Escherichia coli clones, and numerous genes encoding ESBLs and virulence factors (VFs) are plasmid-mediated. We investigated the plasmidic co-localization of ESBL genes and pathovar-associated VF genes isolated in 18 E. coli isolates from faecal samples of diseased cattle. From the rare ESBL-producing E. coli among the various pathovars, no plasmid co-localization was found between VF and blaCTX-M genes on a single plasmid. However, a link between replicon types and VFs was highlighted: EspP was associated with IncFIB and ToxB with IncB/O...
August 30, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27365311/increased-plasmid-copy-number-is-essential-for-yersinia-t3ss-function-and-virulence
#12
He Wang, Kemal Avican, Anna Fahlgren, Saskia F Erttmann, Aaron M Nuss, Petra Dersch, Maria Fallman, Tomas Edgren, Hans Wolf-Watz
Pathogenic bacteria have evolved numerous virulence mechanisms that are essential for establishing infections. The enterobacterium Yersinia uses a type III secretion system (T3SS) encoded by a 70-kilobase, low-copy, IncFII-class virulence plasmid. We report a novel virulence strategy in Y. pseudotuberculosis in which this pathogen up-regulates the plasmid copy number during infection. We found that an increased dose of plasmid-encoded genes is indispensable for virulence and substantially elevates the expression and function of the T3SS...
July 29, 2016: Science
https://www.readbyqxmd.com/read/27363583/active-surveillance-scheme-in-three-romanian-hospitals-reveals-a-high-prevalence-and-variety-of-carbapenamase-producing-gram-negative-bacteria-a-pilot-study-december-2014-to-may-2015
#13
Dorina Timofte, Carmen Valentina Panzaru, Iuliana Elena Maciuca, Maria Dan, Anca Delia Mare, Adrian Man, Felicia Toma
We report the findings of an active surveillance scheme for detection of asymptomatic carriers with carbapenemase-producing Gram-negative bacteria (CP-GNB) in Romanian hospitals. During a pilot study from December 2014 to May 2015, faecal cultures were screened in three hospitals (two large, one medium-size) for patients newly admitted to selected wards or inpatients transferred from other wards to an intensive-care unit. The study revealed a high prevalence of CP-GNB detected in 22/27 and 28/38 of the carbapenem non-susceptible isolates from Hospitals 1 and 3, respectively...
June 23, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/27261267/glutathione-s-transferase-fosa6-of-klebsiella-pneumoniae-origin-conferring-fosfomycin-resistance-in-esbl-producing-escherichia-coli
#14
Qinglan Guo, Adam D Tomich, Christi L McElheny, Vaughn S Cooper, Nicole Stoesser, Minggui Wang, Nicolas Sluis-Cremer, Yohei Doi
OBJECTIVES: The objectives of this study were to elucidate the genetic context of a novel plasmid-mediated fosA variant, fosA6, conferring fosfomycin resistance and to characterize the kinetic properties of FosA6. METHODS: The genome of fosfomycin-resistant Escherichia coli strain YD786 was sequenced. Homologues of FosA6 were identified through BLAST searches. FosA6 and FosA(ST258) were purified and characterized using a steady-state kinetic approach. Inhibition of FosA activity was examined with sodium phosphonoformate...
September 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27183358/characterization-of-the-genetic-environment-of-the-blakpc-2-gene-among-klebsiella-pneumoniae-isolates-from-a-chinese-hospital
#15
Pinghua Shen, Ying Zhang, Gang Li, Xiaofei Jiang
Infection caused by carbapenem-resistant Klebsiella pneumoniae has become a major healthcare threat and KPC-2 enzyme is a dominant factor mediating carbapenems resistance in K. pneumoniae. This study was designed to determine the genetic environment of blaKPC-2, which prevailed in clinical K. pneumoniae isolates recovered in Huashan Hospital, Shanghai, China. Forty-two clinical isolates were included in this study by blaKPC-2 screening. After multilocus sequence typing and plasmid analyses of PCR-based replicon typing (PBRT), junction PCR, mapping PCR and crossing PCR assays, primer walking, and amplicon sequencing were used to analyze the genetic environment of the blaKPC-2 gene...
July 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27139028/persistent-spread-of-the-rmtb-16s-rrna-methyltransferase-gene-among-escherichia-coli-isolates-from-diseased-food-producing-animals-in-china
#16
Jing Xia, Jian Sun, Ke Cheng, Liang Li, Liang-Xing Fang, Meng-Ting Zou, Xiao-Ping Liao, Ya-Hong Liu
A total of 963 non-duplicate Escherichia coli strains isolated from food-producing animals between 2002 and 2012 were screened for the presence of the 16S rRNA methyltransferase genes. Among the positive isolates, resistance determinants to extended spectrum β-lactamases, plasmid-mediated quinolone resistance genes as well as floR and fosA/A3/C2 were detected using PCR analysis. These isolates were further subjected to antimicrobial susceptibility testing, molecular typing, PCR-based plasmid replicon typing and plasmid analysis...
May 30, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27067314/complete-sequence-of-the-fii-plasmid-p42-2-carrying-blactx-m-55-oqxab-fosa3-and-flor-from-escherichia-coli
#17
Qiu E Yang, Timothy Rutland Walsh, Bao Tao Liu, Meng Ting Zou, Hui Deng, Liang Xing Fang, Xiao Ping Liao, Jian Sun, Ya Hong Liu
We sequenced a novel conjugative multidrug resistance IncF plasmid, p42-2, isolated from Escherichia coli strain 42-2, previously identified in China. p42-2 is 106,886 bp long, composed of a typical IncFII-type backbone (∼54 kb) and one distinct acquired DNA region spanning ∼53 kb, harboring 12 antibiotic resistance genes [blaCTX-M-55, oqxA, oqxB, fosA3, floR, tetA(A), tetA(R), strA, strB, sul2, aph(3')-II, and ΔblaTEM-1]. The spread of these multidrug resistance determinants on the same plasmid is of great concern and, because of coresistance to antibiotics from different classes, is therapeutically challenging...
July 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27006459/evolutionary-history-of-the-global-emergence-of-the-escherichia-coli-epidemic-clone-st131
#18
Nicole Stoesser, Anna E Sheppard, Louise Pankhurst, Nicola De Maio, Catrin E Moore, Robert Sebra, Paul Turner, Luke W Anson, Andrew Kasarskis, Elizabeth M Batty, Veronica Kos, Daniel J Wilson, Rattanaphone Phetsouvanh, David Wyllie, Evgeni Sokurenko, Amee R Manges, Timothy J Johnson, Lance B Price, Timothy E A Peto, James R Johnson, Xavier Didelot, A Sarah Walker, Derrick W Crook
UNLABELLED: Escherichia colisequence type 131 (ST131) has emerged globally as the most predominant extraintestinal pathogenic lineage within this clinically important species, and its association with fluoroquinolone and extended-spectrum cephalosporin resistance impacts significantly on treatment. The evolutionary histories of this lineage, and of important antimicrobial resistance elements within it, remain unclearly defined. This study of the largest worldwide collection (n= 215) of sequenced ST131E...
March 22, 2016: MBio
https://www.readbyqxmd.com/read/26888909/atypical-epidemiology-of-ctx-m-15-among-enterobacteriaceae-from-a-high-diversity-of-non-clinical-niches-in-angola
#19
T G Ribeiro, Â Novais, L Peixe, E Machado
OBJECTIVES: The objective of this study was to investigate the distribution and molecular epidemiology of ESBLs, acquired AmpCs and carbapenemases in Enterobacteriaceae from non-clinical niches in Angola, an under-researched sub-Saharan country. METHODS: Eighty-one samples were recovered from healthy persons (n = 18), healthy animals (n = 33) and their environments (n = 10) or aquatic settings (n = 20) in south Angola (2013). Samples were plated onto CHROMagar™ Orientation with/without antibiotics...
May 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/26856843/molecular-characterization-of-escherichia-coli-strains-isolated-from-retail-meat-that-harbor-blactx-m-and-fosa3-genes
#20
Miaomiao Xie, Dachuan Lin, Kaichao Chen, Edward Wai Chi Chan, Wen Yao, Sheng Chen
A total of 55 cefotaxime-resistantEscherichia coliisolates were obtained from retail meat products purchased in Shenzhen, China, during the period November 2012 to May 2013. Thirty-seven of these 55 isolates were found to harbor ablaCTX-Mgene, with theblaCTX-M-1group being the most common type.blaCMY-2was detected in 16 isolates, alone or in combination with other extended-spectrum β-lactamase (ESBL) determinants. Importantly, thefosA3gene, which encodes fosfomycin resistance, was detected in 12 isolates, with several being found to reside in the conjugative plasmid that harbored theblaCTX-Mgene...
April 2016: Antimicrobial Agents and Chemotherapy
keyword
keyword
24086
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"