keyword
MENU ▼
Read by QxMD icon Read
search

Carattoli

keyword
https://www.readbyqxmd.com/read/27297483/travelers-can-import-colistin-resistant-enterobacteriaceae-including-those-possessing-the-plasmid-mediated-mcr-1-gene
#1
Odette J Bernasconi, Esther Kuenzli, João Pires, Regula Tinguely, Alessandra Carattoli, Christoph Hatz, Vincent Perreten, Andrea Endimiani
Stool samples from 38 travelers returning from India were screened for extended-spectrum cephalosporin- and carbapenem-resistant Enterobacteriaceae implementing standard selective plates. Twenty-six (76.3%) people were colonized with CTX-M or DHA producers, but none of the strains was colistin resistant and/or mcr-1 positive. Nevertheless, using overnight enrichment and CHROMagar Orientation plates supplemented with colistin, four people (10.5%) were found to be colonized with colistin-resistant Escherichia coli One cephalosporin-susceptible sequence type 10 (ST10) strain carried a 4,211-bp ISApl1-mcr-1-ISApl1 element in an IncHI2 plasmid backbone...
August 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27284988/isolation-of-kpc-3-producing-enterobacter-aerogenes-in-a-patient-colonized-by-mdr-klebsiella-pneumoniae
#2
Carolina Venditti, Laura Villa, Alessandro Capone, Daniela Fortini, Silvia D'Arezzo, Carla Nisii, Eugenio Bordi, Vincenzo Puro, Mario Antonini, Alessandra Carattoli, Maria Adriana Cataldo, Nicola Petrosillo, Antonino Di Caro
We describe the interspecies transmission of the plasmid-mediated blaKPC-3 gene, which confers carbapenem resistance, between clinically relevant gram-negative bacteria in a single patient. A KPC-3 producing Enterobacter aerogenes was isolated from a hospitalized patient previously colonized and then infected by a Klebsiella pneumoniae ST101 carrying the blaKPC-3 gene. The strains showed identical plasmids. Since intense horizontal exchanges among bacteria can occur in the gut, clinicians should be aware that patients colonized by carbapenem-resistant K...
June 10, 2016: New Microbiologica
https://www.readbyqxmd.com/read/27056222/complete-genome-sequence-of-kpc-3-and-ctx-m-15-producing-klebsiella-pneumoniae-sequence-type-307
#3
Laura Villa, Claudia Feudi, Daniela Fortini, Michele Iacono, Celestino Bonura, Andrea Endimiani, Caterina Mammina, Alessandra Carattoli
Klebsiella pneumoniaesequence type (ST) 307, carryingblaKPC-3,blaCTX-M-15,blaOXA-1,aac(6')-Ib-cr, andqnrB1 genes, is replacing the predominant hyperepidemic ST258 clone in Italy. Whole-genome and complete plasmid sequencing of one ST307 strain was performed and new features were identified.
2016: Genome Announcements
https://www.readbyqxmd.com/read/26961094/escherichia-coli-an-old-friend-with-new-tidings
#4
J Vila, E Sáez-López, J R Johnson, U Römling, U Dobrindt, R Cantón, C G Giske, T Naas, A Carattoli, M Martínez-Medina, J Bosch, P Retamar, J Rodríguez-Baño, F Baquero, S M Soto
Escherichia coli is one of the most-studied microorganisms worldwide but its characteristics are continually changing. Extraintestinal E. coli infections, such as urinary tract infections and neonatal sepsis, represent a huge public health problem. They are caused mainly by specialized extraintestinal pathogenic E. coli (ExPEC) strains that can innocuously colonize human hosts but can also cause disease upon entering a normally sterile body site. The virulence capability of such strains is determined by a combination of distinctive accessory traits, called virulence factors, in conjunction with their distinctive phylogenetic background...
March 8, 2016: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/26525794/double-copies-of-bla-kpc-3-tn4401a-on-an-incx3-plasmid-in-klebsiella-pneumoniae-successful-clone-st512-from-italy
#5
Daniela Fortini, Laura Villa, Claudia Feudi, João Pires, Celestino Bonura, Caterina Mammina, Andrea Endimiani, Alessandra Carattoli
A carbapenem-resistant sequence type 512 (ST512) Klebsiella pneumoniae carbapenemase 3 (KPC-3)-producing K. pneumoniae strain showing a novel variant plasmid content was isolated in Palermo, Italy, in 2014. ST512 is a worldwide successful clone associated with the spread of bla(KPC) genes located on the IncFIIk pKpQIL plasmid. In our ST512 strain, the bla(KPC-3) gene was unusually located on an IncX3 plasmid, whose complete sequence was determined. Two copies of bla(KPC-3)::Tn4401a caused by intramolecular transposition events were detected in the plasmid...
January 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26442177/integration-of-erm-b-containing-elements-through-large-chromosome-fragment-exchange-in-clostridium-difficile
#6
François Wasels, Patrizia Spigaglia, Fabrizio Barbanti, Marc Monot, Laura Villa, Bruno Dupuy, Alessandra Carattoli, Paola Mastrantonio
In Clostridium difficile, erm(B) genes are located on mobile elements like Tn5398 and Tn6215. In previous studies, some of these elements were transferred by conjugation-like mechanisms, mobilized in trans by helper conjugative systems. In this study, we analyzed the genomes of several recipient strains that acquired either Tn5398 or Tn6215-like elements. We demonstrated that the integration of the transposons in the genome of the recipient cell was always due to homologous recombination events, involving exchange of large chromosomal segments...
January 2015: Mobile Genetic Elements
https://www.readbyqxmd.com/read/26248381/characterization-of-an-enterobacter-cloacae-strain-producing-both-kpc-and-ndm-carbapenemases-by-whole-genome-sequencing
#7
Wenjing Wu, Yu Feng, Alessandra Carattoli, Zhiyong Zong
A carbapenem-resistant Enterobacter cloacae strain, WCHECl-14653, causing a fatal bloodstream infection, was characterized by genome sequencing and conjugation experiments. The strain carried two carbapenemase genes, blaNDM-1 and blaKPC-2, on separate IncF plasmids. The coexistence of blaNDM-1 and blaKPC-2 conferred slightly higher-level carbapenem resistance compared with that of blaNDM-1 or blaKPC-2 alone, and the coexistence of two IncF plasmids may generate new platforms for spreading carbapenemase genes...
October 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26169417/inca-c-plasmid-carrying-bla-ndm-1-bla-cmy-16-and-fosa3-in-a-salmonella-enterica-serovar-corvallis-strain-isolated-from-a-migratory-wild-bird-in-germany
#8
L Villa, B Guerra, S Schmoger, J Fischer, R Helmuth, Z Zong, A García-Fernández, A Carattoli
A Salmonella enterica serovar Corvallis strain was isolated from a wild bird in Germany. This strain carried the IncA/C2 pRH-1238 plasmid. Complete sequencing of the plasmid was performed, identifying the blaNDM-1, blaCMY-16, fosA3, sul1, sul2, strA, strB, aac(6')-Ib, aadA5, aphA6, tetA(A), mphA, floR, dfrA7, and merA genes, which confer clinically relevant resistance to most of the antimicrobial classes, including β-lactams with carbapenems, fosfomycin, aminoglycosides, co-trimoxazole, tetracyclines, and macrolides...
October 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/25933288/differentiation-of-incl-and-incm-plasmids-associated-with-the-spread-of-clinically-relevant-antimicrobial-resistance
#9
Alessandra Carattoli, Salome N Seiffert, Sybille Schwendener, Vincent Perreten, Andrea Endimiani
INTRODUCTION: blaOXA-48, blaNDM-1 and blaCTX-M-3 are clinically relevant resistance genes, frequently associated with the broad-host range plasmids of the IncL/M group. The L and M plasmids belong to two compatible groups, which were incorrectly classified together by molecular methods. In order to understand their evolution, we fully sequenced four IncL/M plasmids, including the reference plasmids R471 and R69, the recently described blaOXA-48-carrying plasmid pKPN-El.Nr7 from a Klebsiella pneumoniae isolated in Bern (Switzerland), and the blaSHV-5 carrying plasmid p202c from a Salmonella enterica from Tirana (Albania)...
2015: PloS One
https://www.readbyqxmd.com/read/25123809/emergence-of-klebsiella-pneumoniae-co-producing-ndm-1-oxa-48-ctx-m-15-cmy-16-qnra-and-arma-in-switzerland
#10
Salome N Seiffert, Jonas Marschall, Vincent Perreten, Alessandra Carattoli, Hansjakob Furrer, Andrea Endimiani
Extensively drug-resistant (XDR) Klebsiella pneumoniae isolates usually carry a single carbapenemase (e.g. KPC, NDM, OXA-48-like). Here we describe an XDR K. pneumoniae of sequence type 101 that was detected in the screening rectal swab of a patient transferred from the intensive care unit of a hospital located in Belgrade (Serbia) to Bern University Hospital (Switzerland). The isolate was resistant to all antibiotics with the exception of colistin [minimum inhibitory concentration] (MIC ≤ 0.125 μg/mL), tigecycline (MIC = 0...
September 2014: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/25092703/high-prevalence-of-extended-spectrum-%C3%AE-lactamase-plasmid-mediated-ampc-and-carbapenemase-genes-in-pet-food
#11
Salome N Seiffert, Alessandra Carattoli, Regula Tinguely, Agnese Lupo, Vincent Perreten, Andrea Endimiani
We evaluated the pet food contained in 30 packages as a potential origin of extended-spectrum cephalosporin-resistant Gram-negative organisms and β-lactamase genes (bla). Live bacteria were not detected by selective culture. However, PCR investigations on food DNA extracts indicated that samples harbored the blaCTX-M-15 (53.3%), blaCMY-4 (20%), and blaVEB-4-like (6.7%) genes. Particularly worrisome was the presence of blaOXA-48-like carbapenemases (13.3%). The original pet food ingredients and/or the production processes were highly contaminated with bacteria carrying clinically relevant acquired bla genes...
October 2014: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/24957829/long-term-dissemination-of-ctx-m-5-producing-hypermutable-salmonella-enterica-serovar-typhimurium-sequence-type-328-strains-in-russia-belarus-and-kazakhstan
#12
Varvara K Kozyreva, Elena N Ilina, Maja V Malakhova, Alessandra Carattoli, Ilya S Azizov, Dmitry V Tapalski, Roman S Kozlov, Mikhail V Edelstein
In this paper, we present evidence of long-term circulation of cefotaxime-resistant clonally related Salmonella enterica serovar Typhimurium strains over a broad geographic area. The genetic relatedness of 88 isolates collected from multiple outbreaks and sporadic cases of nosocomial salmonellosis in various parts of Russia, Belarus, and Kazakhstan from 1996 to 2009 was established by multilocus tandem-repeat analysis (MLVA) and multilocus sequence typing (MLST). The isolates belong to sequence type 328 (ST328) and produce CTX-M-5 β-lactamase, whose gene is carried by highly related non-self-conjugative but mobilizable plasmids...
September 2014: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/24862095/complete-sequences-of-inchi1-plasmids-carrying-blactx-m-1-and-qnrs1-in-equine-escherichia-coli-provide-new-insights-into-plasmid-evolution
#13
Monika Dolejska, Laura Villa, Marco Minoia, Luca Guardabassi, Alessandra Carattoli
OBJECTIVES: To determine the structure of two multidrug-resistant IncHI1 plasmids carrying blaCTX-M-1 in Escherichia coli isolates disseminated in an equine clinic in the Czech Republic. METHODS: A complete nucleotide sequencing of 239 kb IncHI1 (pEQ1) and 287 kb IncHI1/X1 (pEQ2) plasmids was performed using the 454-Genome Sequencer FLX system. The sequences were compared using bioinformatic tools with other sequenced IncHI1 plasmids. RESULTS: A comparative analysis of pEQ1 and pEQ2 identified high nucleotide identity with the IncHI1 type 2 plasmids...
September 2014: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/24777092/in-silico-detection-and-typing-of-plasmids-using-plasmidfinder-and-plasmid-multilocus-sequence-typing
#14
Alessandra Carattoli, Ea Zankari, Aurora García-Fernández, Mette Voldby Larsen, Ole Lund, Laura Villa, Frank Møller Aarestrup, Henrik Hasman
In the work presented here, we designed and developed two easy-to-use Web tools for in silico detection and characterization of whole-genome sequence (WGS) and whole-plasmid sequence data from members of the family Enterobacteriaceae. These tools will facilitate bacterial typing based on draft genomes of multidrug-resistant Enterobacteriaceae species by the rapid detection of known plasmid types. Replicon sequences from 559 fully sequenced plasmids associated with the family Enterobacteriaceae in the NCBI nucleotide database were collected to build a consensus database for integration into a Web tool called PlasmidFinder that can be used for replicon sequence analysis of raw, contig group, or completely assembled and closed plasmid sequencing data...
July 2014: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/24379204/genomics-of-kpc-producing-klebsiella-pneumoniae-sequence-type-512-clone-highlights-the-role-of-ramr-and-ribosomal-s10-protein-mutations-in-conferring-tigecycline-resistance
#15
Laura Villa, Claudia Feudi, Daniela Fortini, Aurora García-Fernández, Alessandra Carattoli
Full genome sequences were determined for five Klebsiella pneumoniae strains belonging to the sequence type 512 (ST512) clone, producing KPC-3. Three strains were resistant to tigecycline, one showed an intermediate phenotype, and one was susceptible. Comparative analysis performed using the genome of the susceptible strain as a reference sequence identified genetic differences possibly associated with resistance to tigecycline. Results demonstrated that mutations in the ramR gene occurred in two of the three sequenced strains...
2014: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/24256888/isolation-of-ndm-1-producing-pseudomonas-aeruginosa-sequence-type-st235-from-a-stem-cell-transplant-patient-in-italy-may-2013
#16
A Carattoli, D Fortini, R Galetti, A Garcia-Fernandez, G Nardi, D Orazi, A Capone, I Majolino, A Proia, B Mariani, G Parisi, A Morrone, N Petrosillo
We describe the first isolation of an NDM-1-producing Pseudomonas aeruginosa in Italy. In May 2013, a patient with acute lymphoblastic leukaemia and history of prior hospitalisation in Belgrad, Serbia, underwent stem cell transplantation at a tertiary care hospital in Rome, Italy. After transplantion, sepsis by NDM-1-producing P. aeruginosa occurred, leading to septic shock and fatal outcome.
2013: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/24219100/high-prevalence-of-oqxab-in-escherichia-coli-isolates-from-domestic-and-wild-lagomorphs-in-italy
#17
Giorgia Dotto, Martina Giacomelli, Guido Grilli, Viviana Ferrazzi, Alessandra Carattoli, Daniela Fortini, Alessandra Piccirillo
This study aimed to identify and characterize class 1 and 2 integrons and plasmid-mediated quinolones resistance (PMQR) genes in a collection of 113 multidrug resistance (MDR) Escherichia coli isolated from farm and wild lagomorphs between 2006 and 2008 in Northern Italy. Strains were examined for antimicrobial susceptibility by agar disk diffusion method and E-test for colistin (COL); integrons and gene cassettes content by real-time polymerase chain reaction (PCR) and DNA sequencing; PMQR genes by PCR and DNA sequencing; clonal relatedness by multilocus sequence typing; and plasmids by PCR-based replicon typing...
April 2014: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/23800906/reversion-to-susceptibility-of-a-carbapenem-resistant-clinical-isolate-of-klebsiella-pneumoniae-producing-kpc-3
#18
Laura Villa, Alessandro Capone, Daniela Fortini, Monika Dolejska, Irene Rodríguez, Fabrizio Taglietti, Paolo De Paolis, Nicola Petrosillo, Alessandra Carattoli
OBJECTIVES: We report the case of a kidney-transplant patient, suffering an intra-abdominal abscess at the surgical site caused by a carbapenem-resistant ST258 Klebsiella pneumoniae clone, producing the KPC-3 carbapenemase. Under tigecycline treatment, the patient developed a sepsis caused by a carbapenem-susceptible ST258 K. pneumoniae strain. Complete DNA sequences of the plasmids carried by the resistant and susceptible strains from this patient were determined. METHODS: The complete DNA sequences of plasmids were obtained by applying the 454 Genome Sequencer FLX-PLUS procedure on a library constructed of total plasmid DNA purified from the carbapenem-resistant and -susceptible strains...
November 2013: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/23586437/molecular-characterization-of-multiresistant-escherichia-coli-producing-or-not-extended-spectrum-%C3%AE-lactamases
#19
Belén Ruiz del Castillo, Laura Vinué, Elena Jesús Román, Beatriz Guerra, Alessandra Carattoli, Carmen Torres, Luis Martínez-Martínez
BACKGROUND: The prevalence and type of plasmids, resistance genes and integrons carried by two collections of multiresistant E. coli producing or not extended-spectrum β-lactamases have been compared. Rep-PCR was used to determine the clonal relationship of the organisms. Plasmids were classified according to their incompatibility. Class 1 and Class 2 integrons and antibiotic resistance genes were analysed by PCR and sequencing. RESULTS: Both collections of organisms contained a large diversity of unrelated strains with some clones distributed in both groups of isolates...
2013: BMC Microbiology
https://www.readbyqxmd.com/read/23586392/rapid-plasmid-replicon-typing-by-real-time-pcr-melting-curve-analysis
#20
Maikel Boot, Susanne Raadsen, Paul H M Savelkoul, Christina Vandenbroucke-Grauls
BACKGROUND: Genes encoding Extended Spectrum Beta Lactamases are usually located on transferable plasmids. Each plasmid contains its own replication mechanism. Carattoli et al. developed an extended PCR-based replicon typing method to characterize and identify the replicons of the major plasmid incompatibility groups in Enterobacteriaceae. Based on this method, we designed a rapid approach with amplicon detection by real-time melting curve analysis. This method appeared to be fast, sensitive, less laborious, less prone to contamination and applicable in a routine laboratory environment...
2013: BMC Microbiology
keyword
keyword
24082
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"