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https://www.readbyqxmd.com/read/28195222/rna-seq-based-detection-of-differentially-expressed-genes-in-the-skeletal-muscle-of-duroc-pigs-with-distinct-lipid-profiles
#1
T F Cardoso, A Cánovas, O Canela-Xandri, R González-Prendes, M Amills, R Quintanilla
We have used a RNA-seq approach to investigate differential expression in the skeletal muscle of swine (N = 52) with divergent lipid profiles i.e. HIGH (increased intramuscular fat and muscle saturated and monounsaturated fatty acid contents, higher serum lipid concentrations and fatness) and LOW pigs (leaner and with an increased muscle polyunsaturated fatty acid content). The number of mRNAs and non-coding RNAs (ncRNAs) expressed in the porcine gluteus medius muscle were 18,104 and 1,558, respectively...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28193782/mitochondrial-mass-and-activity-as-a-function-of-body-composition-in-individuals-with-spinal-cord-injury
#2
Laura C O'Brien, Rodney C Wade, Liron Segal, Qun Chen, Jeannie Savas, Edward J Lesnefsky, Ashraf S Gorgey
Spinal cord injury (SCI) is accompanied by deterioration in body composition and severe muscle atrophy. These changes put individuals at risk for insulin resistance, type II diabetes, and cardiovascular disease. To determine the relationships between skeletal muscle mitochondrial mass, activity, and body composition, 22 men with motor complete SCI were studied. Body composition assessment was performed using dual-energy X-ray absorptiometry and magnetic resonance imaging. Skeletal muscle biopsies were obtained from the vastus lateralis muscle to measure citrate synthase (CS) and complex III (CIII) activity...
February 2017: Physiological Reports
https://www.readbyqxmd.com/read/28191379/functional-and-aesthetic-recovery-of-congenital-muscular-torticollis-with-intramuscular-stromal-vascular-fraction-enriched-fat-grafting
#3
Juan Monreal
Congenital muscular torticollis is a well-known pathological condition caused by the contracture and shortening of the sternocleidomastoid muscle. This condition is manifested by a neck postural deformity often accompanied by some kind of facial asymmetry. Once diagnosed, treatment by early physiotherapy is generally successful in a high percentage of patients if performed during the first year of life. Later, especially after the fourth year, conservative treatment is usually far less effective, and surgical techniques remain the only way to improve neck contour and function...
January 12, 2017: Curēus
https://www.readbyqxmd.com/read/28183305/controlling-of-growth-performance-lipid-deposits-and-fatty-acid-composition-of-chicken-meat-through-a-probiotic-lactobacillus-johnsonii-during-subclinical-clostridium-perfringens-infection
#4
Hesong Wang, Xueqin Ni, Lei Liu, Dong Zeng, Jing Lai, Xiaodan Qing, Guangyao Li, Kangcheng Pan, Bo Jing
BACKGROUND: Meat is considered as a major source of polyunsaturated fatty acid (PUFA) which is essential for humans, therefore its lipid level and fatty acid composition have drawn great attention. As no clinical sign can be found in chicks subclinically infected by Clostridium perfringens (CP), the meat may be purchased and eaten. The objective of the present study was to determine whether Lactobacillus johnsonii (LJ) can control the CP-caused impact on growth, lipid levels, fatty acid composition and other flavor or nutritional quality in the meat...
February 10, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28183280/whole-genome-scan-reveals-the-genetic-signature-of-african-ankole-cattle-breed-and-potential-for-higher-quality-beef
#5
Mengistie Taye, Jaemin Kim, Sook Hee Yoon, Wonseok Lee, Olivier Hanotte, Tadelle Dessie, Stephen Kemp, Okeyo Ally Mwai, Kelsey Caetano-Anolles, Seoae Cho, Sung Jong Oh, Hak-Kyo Lee, Heebal Kim
BACKGROUND: Africa is home to numerous cattle breeds whose diversity has been shaped by subtle combinations of human and natural selection. African Sanga cattle are an intermediate type of cattle resulting from interbreeding between Bos taurus and Bos indicus subspecies. Recently, research has asserted the potential of Sanga breeds for commercial beef production with better meat quality as compared to Bos indicus breeds. Here, we identified meat quality related gene regions that are positively selected in Ankole (Sanga) cattle breeds as compared to indicus (Boran, Ogaden, and Kenana) breeds using cross-population (XP-EHH and XP-CLR) statistical methods...
February 9, 2017: BMC Genetics
https://www.readbyqxmd.com/read/28183279/three-dimensional-comparison-of-intramuscular-fat-content-between-young-and-old-adults
#6
Akito Yoshiko, Maya Hioki, Nana Kanehira, Kiyoshi Shimaoka, Teruhiko Koike, Hisataka Sakakibara, Yoshiharu Oshida, Hiroshi Akima
BACKGROUND: Fat infiltration within skeletal muscle is known as intramuscular fat (IMF), which increases with aging. Studies have assessed IMF content, using the mid-thigh as a representative location. However, three-dimensional IMF distribution is not well understood. The aim of this study was to compare the IMF content in young and old adults by assessing its distribution along the length of the thigh. METHODS: Consecutive transaxial images of the right thighs in 15 young (age, 21...
February 10, 2017: BMC Medical Imaging
https://www.readbyqxmd.com/read/28166119/muscle-protein-turnover-and-the-molecular-regulation-of-muscle-mass-during-hypoxia
#7
Stefan M Pasiakos, Claire E Berryman, Christopher T Carrigan, Andrew J Young, John W Carbone
Effects of environmental hypoxia on fat-free mass are well studied. Negative energy balance, increased nitrogen excretion and fat-free mass loss are commonly observed in lowlanders sojourning at high altitude. Reductions in fat-free mass can be minimized if energy consumption matches energy expenditure. However, in non-research settings, achieving energy balance during high altitude sojourns is unlikely and myofibrillar protein mass is usually lost, but the mechanisms accounting for the loss of muscle mass are not clear...
February 4, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28158957/trunk-muscle-characteristics-of-the-multifidi-erector-spinae-psoas-and-quadratus-lumborum-in-older-adults-with-and-without-chronic-low-back-pain
#8
J Megan Sions, James M Elliott, Ryan T Pohlig, Gregory E Hicks
Study Design Cross-sectional study. Background Muscle support for the trunk is provided by the multifidi, erector spinae, psoas, and quadratus lumborum. Trunk muscle characteristics may be altered with aging and/or chronic LBP. To date, most trunk muscle research has been conducted among younger adults. Given age-related muscle changes, i.e. reduced size and increased intramuscular fat, studies are needed in older adults, including comparisons of older adults with and without LBP. Objective To determine if there are differences in trunk muscle characteristics between older adults with and without chronic low back pain (LBP), while controlling for age, sex, and body mass index...
February 3, 2017: Journal of Orthopaedic and Sports Physical Therapy
https://www.readbyqxmd.com/read/28131819/bovine-lipid-metabolism-related-gene-gpam-molecular-characterization-function-identification-and-association-analysis-with-fat-deposition-traits
#9
Haibin Yu, Zhihui Zhao, Xianzhong Yu, Junya Li, Chuanyan Lu, Runjun Yang
Glycerol-3-phosphate acyltransferase mitochondrial (GPAM) is the enzyme that catalyzes the initial and committed step of glycerolipid synthesis in animal lipometabolism related pathway. In the present study, the correlation of GPAM expression level with adipogenesis was examined in vitro by RNAi and gene expression to silencing and over-expression of GPAM gene in BEF cells. And then, 2 novel polymorphisms were identified within key functional domain of bovine GPAM gene by sequencing and the relationship between variants of GPAM gene with fat deposition traits of Chinese Simmental-cross steers was analyzed using statistical methods...
January 25, 2017: Gene
https://www.readbyqxmd.com/read/28123083/effects-of-balanced-selection-for-intramuscular-fat-and-abdominal-fat-percentage-and-estimates-of-genetic-parameters
#10
M Jiang, W L Fan, S Y Xing, J Wang, P Li, R R Liu, Q H Li, M Q Zheng, H X Cui, J Wen, G P Zhao
Intramuscular fat (IMF) content contributes to meat flavor and improves meat quality. Excessive abdominal fat, however, leads to a waste of feed resources. Here, an independent up-selection for IMF was used as a control (Line C), and a balanced selection program, with up-selection for IMF and down-selection AFP (Line B), was studied in JingXing yellow chickens. The mean of IMF and AFP within a family was the phenotypic value upon which selection was based. The selective pressures of IMF in line B and line C were the same in each generation...
February 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28103301/differences-in-beef-quality-between-angus-bos-taurus-taurus-and-nellore-bos-taurus-indicus-cattle-through-a-proteomic-and-phosphoproteomic-approach
#11
Rafael Torres de Souza Rodrigues, Mario Luiz Chizzotti, Camilo Elber Vital, Maria Cristina Baracat-Pereira, Edvaldo Barros, Karina Costa Busato, Rafael Aparecido Gomes, Márcio Machado Ladeira, Taiane da Silva Martins
Proteins are the major constituents of muscle and are key molecules regulating the metabolic changes during conversion of muscle to meat. Brazil is one of the largest exporters of beef and most Brazilian cattle are composed by zebu (Nellore) genotype. Bos indicus beef is generally leaner and tougher than Bos taurus such as Angus. The aim of this study was to compare the muscle proteomic and phosphoproteomic profile of Angus and Nellore. Seven animals of each breed previously subjected the same growth management were confined for 84 days...
2017: PloS One
https://www.readbyqxmd.com/read/28067368/the-effect-of-arginine-on-the-wnt-%C3%AE-catenin-signaling-pathway-during-porcine-intramuscular-preadipocyte-differentiation
#12
Xiaoling Chen, Yanliu Luo, Gang Jia, Guangmang Liu, Hua Zhao, Zhiqing Huang
Dietary l-arginine supplementation decreases backfat thickness and increases intramuscular fat content in growing-finishing pigs, but the underlying mechanisms are unknown. In this study, the effect of arginine on differentiation of porcine intramuscular preadipocytes was investigated in vitro. We showed that the mRNA and protein expressions of the peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α were upregulated by arginine supplementation. Furthermore, the intracellular triglyceride level was increased by arginine supplementation...
January 9, 2017: Food & Function
https://www.readbyqxmd.com/read/28063249/effects-of-feeding-frequency-on-meat-quality-traits-and-longissimus-muscle-proteome-in-finishing-pigs
#13
J B Liu, X Cai, H Xiong, H F Zhang
Sixty-four barrows with an initial body weight of 59.8 ± 2.1 kg were allocated to one of the two feeding frequency regimes (had free access to diet and fed two meals per day). Pigs had free access (FA) to feed were fed on an ad libitum basis during the 8-week experimental period. Pigs fed twice daily (M2) were allowed to consume their meals in 2 h. Pigs fed twice daily had lower average daily feed intake (p < 0.01) and average daily gain (p < 0.1), but a greater G:F (p < 0.05) than FA pigs...
January 7, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28059854/perforator-phase-contrast-angiography-of-deep-inferior-epigastric-perforators-a-better-preoperative-imaging-tool-for-flap-surgery-than-computed-tomographic-angiography
#14
Xiangyu Yang, Michael J Miller, Harry T Friel, Allison Slijepcevic, Michael V Knopp
OBJECTIVE: The aim of this study was to demonstrate the feasibility of in vivo perforator visualization by a newly proposed magnetic resonance-based perforator phase contrast angiography (pPCA) technique for deep inferior epigastric perforator (DIEP) flap surgery and to prospectively compare its image quality and clinical value with computed tomographic angiography (CTA), the state-of-the-art perforator imaging technique. MATERIALS AND METHODS: Institutional review board approval and informed consent were obtained...
January 4, 2017: Investigative Radiology
https://www.readbyqxmd.com/read/28055958/maternal-butyrate-supplementation-induces-insulin-resistance-associated-with-enhanced-intramuscular-fat-deposition-in-the-offspring
#15
Yanping Huang, Shixing Gao, Jinglong Chen, Elke Albrecht, Ruqian Zhao, Xiaojing Yang
Maternal nutrition is important for the risk of the offspring to develop insulin resistance and adiposity later in life. The study was undertaken to determine effects of maternal butyrate supplementation on lipid metabolism and insulin sensitivity in the offspring skeletal muscle. The offspring of rats, fed a control diet or a butyrate diet (1% sodium butyrate) throughout gestation and lactation, was studied at weaning and at 60 days of age. The offspring of dams fed a butyrate diet had higher HOMA-insulin resistance and impaired glucose tolerance...
December 30, 2016: Oncotarget
https://www.readbyqxmd.com/read/28046172/effect-of-divergent-selection-for-intramuscular-fat-on-sensory-traits-and-instrumental-texture-in-rabbit-meat
#16
M Martínez-Álvaroi, V Penalba, A Blasco, P Hernández
Intramuscular fat (IMF) is one of the main parameters affecting meat quality. This work analyzes the effect of selection for IMF on sensory attributes and instrumental texture parameters in rabbit meat. A total of 115 rabbits after 6 generations of divergent selection for IMF were slaughtered at 9 or 13 wk (57 and 58 animals, respectively). For each animal, the left longissimus dorsi muscle (LD) was analyzed by near-infrared spectroscopy to measure IMF whereas the right LD was used for the sensory or instrumental texture analysis...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/28046152/divergent-selection-on-intramuscular-fat-in-rabbits-responses-to-selection-and-genetic-parameters
#17
M Martínez-Álvaro, P Hernández, A Blasco
A divergent selection experiment on intramuscular fat (IMF) was performed in rabbits. The aim of this study is to estimate the response to selection, the correlated responses in carcass and meat quality traits, and their genetic parameters. Selection criterion was the averaged phenotypic value of IMF measured at 9 wk of age in 2 full-sibs of the candidate. Traits considered were IMF, BW, chilled carcass weight, reference carcass weight, scapular and perirenal fat weights, carcass and meat color, pH, protein and fatty acid composition of meat...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/28038376/lean-muscle-volume-of-the-thigh-has-a-stronger-relationship-with-muscle-power-than-muscle-strength-in-women-with-knee-osteoarthritis
#18
Michael J Davison, Monica R Maly, Peter J Keir, Sandani M Hapuhennedige, Amie T Kron, Jonathan D Adachi, Karen A Beattie
BACKGROUND: Thigh lean muscle and intramuscular fat have been implicated in the impairment of physical function observed in people with knee osteoarthritis. We investigated the relationships of quadriceps and hamstrings intramuscular fat fraction and lean muscle volume with muscle power and strength, controlling for neuromuscular activation, and physical performance in women with knee OA. METHODS: Women (n=20) 55years or older with symptomatic, radiographic knee osteoarthritis underwent a 3...
January 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/28029333/biochemical-and-organoleptic-characteristics-of-muscle-from-early-and-late-maturing-bulls-in-different-production-systems
#19
G B Mezgebo, F J Monahan, M McGee, E G O'Riordan, B Picard, R I Richardson, A P Moloney
In grass-based beef production systems (PS), early maturing (EM) breed types may be preferable to late maturing (LM) breed types in achieving adequate carcass fat cover. Biochemical and organoleptic characteristics of muscle from suckler bulls were investigated in EM and LM (n=28/breed) assigned to one of two PS (ad libitum concentrates and grass silage to slaughter (C) or ad libitum silage plus 2 kg concentrate daily during winter followed by 99 days at pasture and then an indoor finishing period on C (GSPC)) in a 2 breed type×2 PS factorial arrangement of treatments...
December 28, 2016: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/27994193/association-and-expression-analyses-of-the-ucp2-and-ucp3-gene-polymorphisms-with-body-measurement-and-meat-quality-traits-in-qinchuan-cattle
#20
Yaning Wang, Wucai Yang, Linsheng Gui, Hongbao Wang, Linsen Zan
The uncoupling proteins (UCPs) belong to the mitochondrial inner membrane anion carrier superfamily and play an important role in energy homeostasis. Genetic studies have demonstrated that Ucp2 and Ucp3 gene variants are involved in obesity and metabolic syndrome. The aim of this study was to identify associations between polymorphisms of Ucp2 and Ucp3 genes and economically-important traits in Qinchuan cattle. In the present study, one single-nucleotide polymorphism (SNP) in the 5'UTR region (SNP1:g.C-754G) of the Ucp2 gene was identified by direct sequencing of 441 Qinchuan cattle...
December 2016: Journal of Genetics
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