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https://www.readbyqxmd.com/read/28528117/composite-elastomeric-polyurethane-scaffolds-incorporating-small-intestinal-submucosa-for-soft-tissue-engineering
#1
Lincui Da, Mei Gong, Anjing Chen, Yi Zhang, Yizhou Huang, Zhijun Guo, Shengfu Li, Jesse Li-Ling, Li Zhang, Huiqi Xie
Although soft tissue replacement has been clinically successful in many cases, the corresponding procedure has many limitations including the lack of resilience and mechanical integrity, significant donor-site morbidity, volume loss with time, and fibrous capsular contracture. These disadvantages can be alleviated by utilizing bio-absorbable scaffolds with high resilience and large strain, which are capable of stimulating natural tissue regeneration. Hence, the chemically crosslinked tridimensional scaffolds obtained by incorporating water-based polyurethane (PU) (which was synthesized from polytetramethylene ether glycol, isophorone diisocyanate, and 2,2-bis(hydroxymethyl) butyric acid) into a bioactive extracellular matrix consisting of small intestinal submucosa (SIS) have been tested in this study to develop a new approach for soft tissue engineering...
May 17, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28527978/enhanced-antimony-v-removal-using-synergistic-effects-of-fe-hydrolytic-flocs-and-ultrafiltration-membrane-with-sludge-discharge-evaluation
#2
Baiwen Ma, Xing Wang, Ruiping Liu, Zenglu Qi, William A Jefferson, Huachun Lan, Huijuan Liu, Jiuhui Qu
The integration of adsorbents with ultrafiltration (UF) membranes is a promising method for alleviating membrane fouling and reducing land use. However, a number of problems have become apparent concerning the granular adsorbents used currently, such as high running cost, high chance of causing membrane surface damage, low in situ chemical cleaning efficiency, etc. Herein, to overcome these disadvantages, loose in situ hydrolyzed flocs were directly injected into the membrane tank, providing strong adsorption ability at low cost...
May 13, 2017: Water Research
https://www.readbyqxmd.com/read/28520408/engineering-and-characterization-of-bacterial-nanocellulose-films-as-low-cost-and-flexible-sensor-material
#3
Rahul K Mangayil, Satu M Rajala, Arno J Pammo, Essi L Sarlin, Jin Luo, Ville P Santala, Matti Tapani Karp, Sampo R Tuukkanen
Some bacterial strains such as Komagataeibacter xylinus are able to produce cellulose as an extracellular matrix. In comparison to wood-based cellulose, bacterial cellulose (BC) holds interesting properties such as biodegradability, high purity, water-holding capacity and superior mechanical and structural properties. Aiming towards improvement in BC production titer and tailored alterations to the BC film, we engineered K. xylinus to overexpress partial and complete bacterial cellulose synthase operon that encodes activities for BC production...
May 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28515712/allelic-variation-in-outer-membrane-protein-a-and-its-influence-on-attachment-of-escherichia-coli-to-corn-stover
#4
Chunyu Liao, Xiao Liang, Fan Yang, Michelle L Soupir, Adina C Howe, Michael L Thompson, Laura R Jarboe
Understanding the genetic factors that govern microbe-sediment interactions in aquatic environments is important for water quality management and reduction of waterborne disease outbreaks. Although chemical properties of bacteria have been identified that contribute to initiation of attachment, the outer membrane proteins that contribute to these chemical properties still remain unclear. In this study we explored the attachment of 78 Escherichia coli environmental isolates to corn stover, a representative agricultural residue...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28515440/a-highly-potent-class-of-halogenated-phenazine-antibacterial-and-biofilm-eradicating-agents-accessed-through-a-modular-wohl-aue-synthesis
#5
Hongfen Yang, Yasmeen Abouelhassan, Gena M Burch, Dimitris Kallifidas, Guangtao Huang, Hussain Yousaf, Shouguang Jin, Hendrik Luesch, Robert W Huigens
Unlike individual, free-floating planktonic bacteria, biofilms are surface-attached communities of slow- or non-replicating bacteria encased within a protective extracellular polymeric matrix enabling persistent bacterial populations to tolerate high concentrations of antimicrobials. Our current antibacterial arsenal is composed of growth-inhibiting agents that target rapidly-dividing planktonic bacteria but not metabolically dormant biofilm cells. We report the first modular synthesis of a library of 20 halogenated phenazines (HP), utilizing the Wohl-Aue reaction, that targets both planktonic and biofilm cells...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28514734/inflammatory-microrna-194-and-515-attenuate-the-biosynthesis-of-chondroitin-sulfate-during-human-intervertebral-disc-degeneration
#6
Bo Hu, Chen Xu, Ye Tian, Changgui Shi, Ying Zhang, Lianfu Deng, Hongyu Zhou, Peng Cao, Huajiang Chen, Wen Yuan
Intervertebral disc degeneration (IDD) is characterized by dehydration and loss of extracellular matrixes in the nucleus pulposus region. Chondroitin sulfate has been found to be the water-binding molecule that played a key role in IDD. Although investigators have reported that inflammatory cytokines are involved in the reduction of chondroitin sulfate in IDD, but the underlying mechanism is unrevealed. Since chondroitin sulfate synthesis is controlled by chondroitin sulfate glycosyltransferases CHSY-1/2/3 and CSGALNACT-1/2, their functional role and regulatory mechanism in IDD is not fully studied...
May 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28512162/increase-in-extracellular-hydration-status-after-initiating-peritoneal-dialysis-electively
#7
Kwanpeemai Panorchan, Andrew Davenport
Renal replacement therapy is designed to treat uremic symptoms and correct hypervolemia. We hypothesized that starting peritoneal dialysis (PD) should reduce overhydration, and we measured body composition and hydration status using bioimpedance in PD patients prior to training and then at the first assessment of peritoneal membrane function. We studied 100 consecutive patients with a planned start to PD, without peritoneal infections or mechanical catheter problems, mean age 54.7 ± 17.1 years, 57% male and 25% diabetic...
May 2017: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
https://www.readbyqxmd.com/read/28511037/role-of-ph-and-ionic-strength-in-the-aggregation-of-tio2-nanoparticles-in-the-presence-of-extracellular-polymeric-substances-from-bacillus-subtilis
#8
Di Lin, S Drew Story, Sharon L Walker, Qiaoyun Huang, Wei Liang, Peng Cai
Increased use of commercial titanium dioxide nanoparticles (TiO2 NPs) in consumer products most likely leads to their additional environmental release. Aggregation and disaggregation processes are expected to play an important role in the fate and transport of TiO2 NPs in natural aquatic ecosystems. Therefore, in this work, we have studied the colloidal stability of TiO2 NPs in the presence of extracellular polymeric substances (EPS) from Bacillus subtilis and the adsorption behavior of EPS on TiO2 NPs in aqueous solutions at different pH values and ionic strengths (IS)...
May 13, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28507171/first-in-man-demonstration-of-direct-endothelin-mediated-natriuresis-and-diuresis
#9
Robert W Hunter, Rebecca Moorhouse, Tariq E Farrah, Iain M MacIntyre, Takae Asai, Peter J Gallacher, Debbie Kerr, Vanessa Melville, Alicja Czopek, Emma E Morrison, Jess R Ivy, James W Dear, Matthew A Bailey, Jane Goddard, David J Webb, Neeraj Dhaun
Endothelin (ET) receptor antagonists are potentially novel therapeutic agents in chronic kidney disease and resistant hypertension, but their use is complicated by sodium and water retention. In animal studies, this side effect arises from ETB receptor blockade in the renal tubule. Previous attempts to determine whether this mechanism operates in humans have been confounded by the hemodynamic consequences of ET receptor stimulation/blockade. We aimed to determine the effects of ET signaling on salt transport in the human nephron by administering subpressor doses of the ET-1 precursor, big ET-1...
May 15, 2017: Hypertension
https://www.readbyqxmd.com/read/28507126/direct-observation-of-structure-and-dynamics-during-phase-separation-of-an-elastomeric-protein
#10
Sean E Reichheld, Lisa D Muiznieks, Fred W Keeley, Simon Sharpe
Despite its growing importance in biology and in biomaterials development, liquid-liquid phase separation of proteins remains poorly understood. In particular, the molecular mechanisms underlying simple coacervation of proteins, such as the extracellular matrix protein elastin, have not been reported. Coacervation of the elastin monomer, tropoelastin, in response to heat and salt is a critical step in the assembly of elastic fibers in vivo, preceding chemical cross-linking. Elastin-like polypeptides (ELPs) derived from the tropoelastin sequence have been shown to undergo a similar phase separation, allowing formation of biomaterials that closely mimic the material properties of native elastin...
May 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28505508/phosphatase-activities-of-a-microepiphytic-community-during-a-bloom-of-ostreopsis-cf-ovata-in-the-northern-adriatic-sea
#11
Stefano Accoroni, Cecilia Totti, Emanuela Razza, Roberta Congestri, Alessandra Campanelli, Mauro Marini, Neil Thomas William Ellwood
It is becoming more apparent that increased organic nutrient loads deriving from anthropogenic activities and natural processes frequently cause the eutrophication of coastal waters. Concurrently, an increasing number of phototrophs have been shown to make use of organic nutrients, mainly through indirect studies of surface enzyme activities or through direct studies of growth in media containing organic-only nutrients. The potential utilization of dissolved organic phosphorus (DOP) by microepiphytic-mats associated with frequently problematic, toxic dinoflagellate Ostreopsis cf...
May 8, 2017: Water Research
https://www.readbyqxmd.com/read/28503851/characterizing-bacterial-communities-in-paper-production-troublemakers-revealed
#12
Anita Zumsteg, Simon K Urwyler, Joachim Glaubitz
Biofilm formation is a major cause of reduced paper quality and increased down time during paper manufacturing. This study uses Illumina next-generation sequencing to identify the microbial populations causing quality issues due to their presence in biofilms and slimes. The paper defects investigated contained traces of the films and/or slime of mainly two genera, Tepidimonas and Chryseobacterium. The Tepidimonas spp. found contributed on average 68% to the total bacterial population. Both genera have been described previously to be associated with biofilms in paper mills...
May 14, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28499183/contribution-of-filamentous-fungi-to-the-musty-odorant-2-4-6-trichloroanisole-in-water-supply-reservoirs-and-associated-drinking-water-treatment-plants
#13
Xiuzhi Bai, Ting Zhang, Zhipeng Qu, Haipu Li, Zhaoguang Yang
In this study, the distribution of 2,4,6-trichloroanisole (2,4,6-TCA) in two water supply reservoirs and four associated drinking water treatment plants (DWTPs) were investigated. The 2,4,6-TCA concentrations were in the range of 1.53-2.36 ng L(-1) in water supply reservoirs and 0.76-6.58 ng L(-1) at DWTPs. To determine the contribution of filamentous fungi to 2,4,6-TCA in a full-scale treatment process, the concentrations of 2,4,6-TCA in raw water, settled water, post-filtration water, and finished water were measured...
May 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/28494781/preparation-and-characterization-of-small-diameter-decellularized-scaffolds-for-vascular-tissue-engineering-in-an-animal-model
#14
Shuangyue Xu, Fangna Lu, Lianna Cheng, Chenglin Li, Xu Zhou, Yuan Wu, Hongxing Chen, Kaichuang Zhang, Lumin Wang, Junjie Xia, Guoliang Yan, Zhongquan Qi
BACKGROUND: The development of a suitable extracellular matrix (ECM) scaffold is the first step in vascular tissue engineering (VTE). Synthetic vascular grafts are available as an alternative to autologous vessels in large-diameter arteries (>8 mm) and medium-diameter arteries (6-8 mm). In small-diameter vessels (<6 mm), synthetic vascular grafts are of limited use due to poor patency rates. Compared with a vascular prosthesis, natural tissue ECM has valuable advantages. Despite considerable progress in recent years, identifying an optimal protocol to create a scaffold for use in small-diameter (<6 mm) fully natural tissue-engineered vascular grafts (TEVG), remains elusive...
May 11, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28493928/nmr-quantification-of-diffusional-exchange-in-cell-suspensions-with-relaxation-rate-differences-between-intra-and-extracellular-compartments
#15
Stefanie Eriksson, Karin Elbing, Olle Söderman, Karin Lindkvist-Petersson, Daniel Topgaard, Samo Lasič
Water transport across cell membranes can be measured non-invasively with diffusion NMR. We present a method to quantify the intracellular lifetime of water in cell suspensions with short transverse relaxation times, T2, and also circumvent the confounding effect of different T2 values in the intra- and extracellular compartments. Filter exchange spectroscopy (FEXSY) is specifically sensitive to exchange between compartments with different apparent diffusivities. Our investigation shows that FEXSY could yield significantly biased results if differences in T2 are not accounted for...
2017: PloS One
https://www.readbyqxmd.com/read/28489283/inflammatory-responses-matrix-remodeling-and-re-epithelialization-after-fractional-co2-laser-treatment-of-scars
#16
Danielle M DeBruler, Britani N Blackstone, Molly E Baumann, Kevin L McFarland, Brian C Wulff, Traci A Wilgus, J Kevin Bailey, Dorothy M Supp, Heather M Powell
BACKGROUND AND OBJECTIVE: Fractional CO2 laser therapy has been used to improve scar pliability and appearance; however, a variety of treatment protocols have been utilized with varied outcomes. Understanding the relationship between laser power and extent of initial tissue ablation and time frame for remodeling could help determine an optimum power and frequency for laser treatment. The characteristics of initial injury caused by fractional CO2 laser treatment, the rates of dermal remodeling and re-epithelialization, and the extent of inflammation as a function of laser stacking were assessed in this study in a porcine scar model...
May 10, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28486777/double-hydrophobic-elastin-like-polypeptides-with-added-functional-motifs-self-assembly-and-cytocompatibility
#17
Duc H T Le, Yoko Tsutsui, Ayae Sugawara-Narutaki, Hiroshi Yukawa, Yoshinobu Baba, Chikara Ohtsuki
We have recently developed a novel double-hydrophobic elastin-like triblock polypeptide called GPG, designed after the uneven distribution of two different hydrophobic domains found in elastin, an extracellular matrix protein providing elasticity and resilience to tissues. Upon temperature trigger, GPG undergoes a sequential self-assembling process to form flexible beaded nanofibers with high homogeneity and excellent dispersibility in water. Given that GPG might be a potential elastin-mimetic material, we sought to explore the biological activities of this block polypeptide...
May 9, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28482533/electrospun-pblg-pla-nanofiber-membrane-for-constructing-in-vitro-3d-model-of-melanoma
#18
Yaping Wang, Junmin Qian, Ting Liu, Weijun Xu, Na Zhao, Aili Suo
Though much progress in utilizing tissue engineering technology to investigate tumor development in vitro has been made, the effective management of human melanoma is still a challenge in clinic due to lack of suitable 3D culture systems. In this study, we prepared a poly(γ-benzyl-l-glutamate)/poly(lactic acid) (PBLG/PLA) nanofiber membrane by electrospinning and demonstrated its suitability as a matrix for 3D culture of melanoma cells in vitro. The electrospun PBLG/PLA nanofiber membrane displayed a smooth and uniform fibrous morphology and had a desirable water contact angle of 79...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28482235/membrane-fouling-by-extracellular-polymeric-substances-after-ozone-pre-treatment-variation-of-nano-particles-size
#19
Wenzheng Yu, Dizhong Zhang, Nigel J D Graham
The application of ozone pre-treatment for ultrafiltration (UF) in drinking water treatment has been studied for more than 10 years, but its performance in mitigating or exacerbating membrane fouling has been inconclusive, and sometimes contradictory. To help explain this, our study considers the significance of the influent organic matter and its interaction with ozone on membrane fouling, using solutions of two representative types of extracellular polymeric substances (EPS), alginate and bovine serum albumin (BSA), and samples of surface water...
May 3, 2017: Water Research
https://www.readbyqxmd.com/read/28476001/impacts-of-rac-and-s-metolachlor-on-cyanobacterial-cell-integrity-and-release-of-microcystins-at-different-nitrogen-levels
#20
Jia Wang, Lijuan Zhang, Jiajia Fan, Yuezhong Wen
Pesticide residues and nitrogen overload (which caused cyanobacteria blooms) have been two serious environmental concerns. In particular, chiral pesticides with different structures may have various impacts on cyanobacteria. Nitrogen may affect the behavior between pesticides and cyanobacteria (e.g., increase the adverse effects of pesticides on cyanobacteria). This study evaluated the impacts of Rac- and S-metolachlor on the cell integrity and toxin release of Microcystis aeruginosa cells at different nitrogen levels...
April 26, 2017: Chemosphere
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