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https://www.readbyqxmd.com/read/27933042/the-role-of-stress-and-stress-adaptations-in-determining-the-fate-of-the-bacterial-pathogen-listeria-monocytogenes-in-the-food-chain
#1
REVIEW
Kerrie NicAogáin, Conor P O'Byrne
The foodborne pathogen Listeria monocytogenes is a highly adaptable organism that can persist in a wide range of environmental and food-related niches. The consumption of contaminated ready-to-eat foods can cause infections, termed listeriosis, in vulnerable humans, particularly those with weakened immune systems. Although these infections are comparatively rare they are associated with high mortality rates and therefore this pathogen has a significant impact on food safety. L. monocytogenes can adapt to and survive a wide range of stress conditions including low pH, low water activity, and low temperature, which makes it problematic for food producers who rely on these stresses for preservation...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27930980/vitamin-d3-intake-as-regulator-of-insulin-degrading-enzyme-and-insulin-receptor-phosphorylation-in-diabetic-rats
#2
Mohamed Mahmoud Elseweidy, Rawia Sarhan Amin, Hebatallah Husseini Atteia, Maha Abdo Ali
Insulin-degrading enzyme (IDE, insulysin) is a rate-limiting enzyme in the insulin degradation process. It is an intracellular 110-kDa thiol zinc-metalloendopeptidase located in the cytosol, peroxisomes, endosomes and cell surface. IDE catalyzes degradation of several small proteins including insulin, amylin and β-amyloid protein. In addition, insulin clearance was expressed as a target in the treatment of type 2 diabetes given the role of hyperinsulinemia in the pathogenesis of insulin resistance. In this study, fourtyadult male Wistar albino rats were used, thirty rats received 20% fructose in drinking water (HFW) for six weeks to induce diabetes...
December 5, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27929105/glymphatic-solute-transport-does-not-require-bulk-flow
#3
Mahdi Asgari, Diane de Zélicourt, Vartan Kurtcuoglu
Observations of fast transport of fluorescent tracers in mouse brains have led to the hypothesis of bulk water flow directed from arterial to venous paravascular spaces (PVS) through the cortical interstitium. At the same time, there is evidence for interstitial solute transport by diffusion rather than by directed bulk fluid motion. It has been shown that the two views may be consolidated by intracellular water flow through astrocyte networks combined with mainly diffusive extracellular transport of solutes...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27923711/characterization-of-na-transport-to-gain-insight-into-the-mechanism-of-acid-base-and-ion-regulation-in-white-sturgeon-acipenser-transmontanus
#4
Ryan B Shartau, Kevin V Brix, Colin J Brauner
Freshwater fish actively take up ions via specific transporters to counter diffusive losses to their hypotonic environment. While much is known about the specific mechanisms employed by teleosts, almost nothing is known about the basal fishes, such as white sturgeon (Acipenser transmontanus) which may offer insight into the evolution of osmo- and ionoregulation in fishes. We investigated Na(+) uptake in juvenile white sturgeon in the presence and absence of transporter inhibitors. We found that sturgeon acclimated to 100μmol 1(-1) Na(+) have Na(+) uptake kinetics typical of teleosts and that a Na(+)/H(+) exchanger (NHE) is the predominant transporter for Na(+) uptake...
December 3, 2016: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/27920892/surface-modification-and-cellular-uptake-evaluation-of-au-coated-ni80fe20-nanodiscs-for-biomedical-applications
#5
Gabriele Barrera, Loredana Serpe, Federica Celegato, Marco Coїsson, Katia Martina, Roberto Canaparo, Paola Tiberto
A nanofabrication technique based on self-assembling of polystyrene nanospheres is used to obtain magnetic Ni80Fe20 nanoparticles with a disc shape. The free-standing nanodiscs (NDs) have diameter and thickness of about 630 nm and 30 nm, respectively. The versatility of fabrication technique allows one to cover the ND surface with a protective gold layer with a thickness of about 5 nm. Magnetization reversal has been studied by room-temperature hysteresis loop measurements in water-dispersed free-standing NDs...
December 6, 2016: Interface Focus
https://www.readbyqxmd.com/read/27919026/hindered-cytoplasmic-diffusion-of-inositol-trisphosphate-restricts-its-cellular-range-of-action
#6
George D Dickinson, Kyle L Ellefsen, Silvina Ponce Dawson, John E Pearson, Ian Parker
The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca(2+)-liberating second messenger inositol trisphosphate (IP3) diffuses with a coefficient (~280 μm(2) s(-1)) similar to that in water, corresponding to a range of action of ~25 μm. Consequently, IP3 is generally considered a "global" cellular messenger. We reexamined this issue by measuring local IP3-evoked Ca(2+) puffs to monitor IP3 diffusing from spot photorelease in neuroblastoma cells...
November 8, 2016: Science Signaling
https://www.readbyqxmd.com/read/27917301/separation-of-bacteria-protozoa-and-carbon-nanotubes-by-density-gradient-centrifugation
#7
Monika Mortimer, Elijah J Petersen, Bruce A Buchholz, Patricia A Holden
Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors. Microbes, as abundant organisms responsible for nutrient cycling in soil and water, are important ecological receptors for studying the effects of CNTs. Quantification of CNT association with microbial cells requires efficient separation of CNT-associated cells from individually dispersed CNTs and CNT agglomerates...
2016: Nanomaterials
https://www.readbyqxmd.com/read/27916883/enhanced-autophagy-in-polycystic-kidneys-of-aqp11-null-mice
#8
Yasuko Tanaka, Mayumi Watari, Tatsuya Saito, Yoshiyuki Morishita, Kenichi Ishibashi
Aquaporin-11 (AQP11) is an intracellular water channel expressed at the endoplasmic reticulum (ER) of the proximal tubule. Its gene disruption in mice leads to intracellular vacuole formation at one week and the subsequent development of polycystic kidneys by three weeks. As the damaged proximal tubular cells with intracellular vacuoles form cysts later, we postulated that autophagy may play a role in the cyst formation and examined autophagy activity before and after cyst development in AQP11(-/-) kidneys...
November 30, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27916562/cryobiological-parameters-of-multipotent-stromal-cells-obtained-from-different-sources
#9
L Lauterboeck, W F Wolkers, B Glasmacher
Stem cells are important for regenerative medicine mainly due to their multilineage differentiation capacity. However, the cells rapidly loose this capability during culturing. Cryopreservation preserves the differentiation potential of the cells, until they are needed. In this study, specific cell properties of multipotent stromal cells (MSCs), from the common marmoset monkey Callithrix jacchus MSCs derived from amnion (Am) and bone marrow (Bm) were studied in order to predict optimal cooling rates for cryopreservation...
December 1, 2016: Cryobiology
https://www.readbyqxmd.com/read/27915187/dose-response-models-and-a-quantitative-microbial-risk-assessment-framework-for-the-mycobacterium-avium-complex-that-account-for-recent-developments-in-molecular-biology-taxonomy-and-epidemiology
#10
REVIEW
Kerry A Hamilton, Mark H Weir, Charles N Haas
Mycobacterium avium complex (MAC) is a group of environmentally-transmitted pathogens of great public health importance. This group is known to be harbored, amplified, and selected for more human-virulent characteristics by amoeba species in aquatic biofilms. However, a quantitative microbial risk assessment (QMRA) has not been performed due to the lack of dose response models resulting from significant heterogeneity within even a single species or subspecies of MAC, as well as the range of human susceptibilities to mycobacterial disease...
November 24, 2016: Water Research
https://www.readbyqxmd.com/read/27909341/intracellular-mechanisms-of-solar-water-disinfection
#11
María Castro-Alférez, María Inmaculada Polo-López, Pilar Fernández-Ibáñez
Solar water disinfection (SODIS) is a zero-cost intervention measure to disinfect drinking water in areas of poor access to improved water sources, used by more than 6 million people in the world. The bactericidal action of solar radiation in water has been widely proven, nevertheless the causes for this remain still unclear. Scientific literature points out that generation of reactive oxygen species (ROS) inside microorganisms promoted by solar light absorption is the main reason. For the first time, this work reports on the experimental measurement of accumulated intracellular ROS in E...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27906617/evaluation-of-cellular-purine-transport-and-metabolism-in-the-caco-2-cell-using-comprehensive-high-performance-liquid-chromatography-method-for-analysis-of-purines
#12
T Fukuuchi, M Kobayashi, N Yamaoka, K Kaneko
Using Caco-2 cells and our previously developed high-performance liquid chromatography method for quantification of purine bases, nucleosides, and nucleotides, we evaluated cellular purine transport and uptake. The analytes were separated using YMC-Triart C18 column with gradient elution. We used Caco-2 cells as intestinal model cells and monitored purine transport across a monolayer for 2 h. The degree of change of purine concentrations in the permeate was very slight; however, it was possible to simultaneously determine these parameters for all purines because of our method's high sensitivity...
December 2016: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/27905583/intracellular-water-an-overlooked-drug-target-cisplatin-impact-in-cancer-cells-probed-by-neutrons
#13
M P M Marques, A L M Batista de Carvalho, V Garcia Sakai, L Hatter, L A E Batista de Carvalho
The first neutron scattering study on human nucleated cells is reported, addressing the subject of solvent-slaving to a drug by probing intracellular water upon drug exposure. Inelastic and quasi-elastic neutron scattering spectroscopy with isotope labelling was applied for monitoring interfacial water response to the anticancer drug cisplatin, in the low prognosis human metastatic breast cancer cells MDA-MB-231. Optical vibrational data were also obtained for lyophilised cells. Concentration-dependent dynamical changes evidencing a progressive mobility reduction were unveiled between untreated and cisplatin-exposed samples, concurrent with variations in the native organisation of water molecules within the intracellular medium as a consequence of drug action...
December 1, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27904274/pretreatment-of-ferulic-acid-protects-human-dermal-fibroblasts-against-ultraviolet-a-irradiation
#14
Hyung Jin Hahn, Ki Bbeum Kim, Seunghee Bae, Byung Gon Choi, Sungkwan An, Kyu Joong Ahn, Su Young Kim
BACKGROUND: Approximately 90%~99% of ultraviolet A (UVA) ray reaches the Earth's surface. The deeply penetrating UVA rays induce the formation of reactive oxygen species (ROS), which results in oxidative stress such as photoproducts, senescence, and cell death. Thus, UVA is considered a primary factor that promotes skin aging. OBJECTIVE: Researchers investigated whether pretreatment with ferulic acid protects human dermal fibroblasts (HDFs) against UVA-induced cell damages...
December 2016: Annals of Dermatology
https://www.readbyqxmd.com/read/27901000/endoplasmic-reticulum-stress-induces-the-early-appearance-of-pro-apoptotic-and-anti-apoptotic-proteins-in-neurons-of-five-familial-alzheimer-s-disease-mice
#15
Hui Shen, Xiao-Dong Pan, Jing Zhang, Yu-Qi Zeng, Meng Zhou, Lu-Meng Yang, Bing Ye, Xiao-Man Dai, Yuan-Gui Zhu, Xiao-Chun Chen
BACKGROUND: Amyloid β (Aβ) deposits and the endoplasmic reticulum stress (ERS) are both well established in the development and progression of Alzheimer's disease (AD). However, the mechanism and role of Aβ-induced ERS in AD-associated pathological progression remain to be elucidated. METHODS: The five familial AD (5×FAD) mice and wild-type (WT) mice aged 2, 7, and 12 months were used in the present study. Morris water maze test was used to evaluate their cognitive performance...
2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27898739/environmental-free-living-amoebae-isolated-from-soil-in-khon-kaen-thailand-antagonize-burkholderia-pseudomallei
#16
Parumon Noinarin, Pisit Chareonsudjai, Pinich Wangsomnuk, Surasak Wongratanacheewin, Sorujsiri Chareonsudjai
Presence of Burkholderia pseudomallei in soil and water is correlated with endemicity of melioidosis in Southeast Asia and northern Australia. Several biological and physico-chemical factors have been shown to influence persistence of B. pseudomallei in the environment of endemic areas. This study was the first to evaluate the interaction of B. pseudomallei with soil amoebae isolated from B. pseudomallei-positive soil site in Khon Kaen, Thailand. Four species of amoebae, Paravahlkampfia ustiana, Acanthamoeba sp...
2016: PloS One
https://www.readbyqxmd.com/read/27898523/role-of-corin-in-the-regulation-of-blood-pressure
#17
Hui Li, Yue Zhang, Qingyu Wu
PURPOSE OF REVIEW: Corin is a transmembrane protease that activates atrial natriuretic peptide (ANP), an important hormone in regulating salt-water balance and blood pressure. This review focuses on the regulation of corin function and potential roles of corin defects in hypertensive, heart, and renal diseases. RECENT FINDINGS: Proprotein convertase subtilisin/kexin-6 has been identified as a primary enzyme that converts zymogen corin to an active protease. Genetic variants that impair corin intracellular trafficking, cell surface expression, and zymogen activation have been found in patients with hypertension, cardiac hypertrophy, and pre-eclampsia...
November 24, 2016: Current Opinion in Nephrology and Hypertension
https://www.readbyqxmd.com/read/27896810/multiple-pathways-for-elevating-extracellular-adenosine-in-the-rat-hippocampal-ca1-region-characterized-by-adenosine-sensor-cells
#18
Kunihiko Yamashiro, Yuki Fujii, Shohei Maekawa, Mitsuhiro Morita
Extracellular adenosine in the brain, which modulates various physiological and pathological processes, fluctuates in a complicated manner that reflects the circadian cycle, neuronal activity, metabolism, and disease states. The dynamics of extracellular adenosine in the brain are not fully understood, largely because of the lack of simple and reliable methods of measuring time-dependent changes in tissue adenosine distribution. This study describes the development of a biosensor, designated an adenosine sensor cell, expressing adenosine A1 receptor, and a genetically modified G protein...
November 5, 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27895856/genetic-characterization-of-cryptosporidium-spp-among-patients-with-gastrointestinal-complaints
#19
Reza Ranjbar, Kaveh Baghaei, Ehsan Nazemalhosseini Mojarad
AIM: This study investigated subtypes of Cryptosporidium in patients with gastrointestinal complaints in Tehran, Iran. BACKGROUND: Cryptosporidium, an intracellular protozean parasite, is among the major causative agents of gastroenteritis disorders in humans. It also causes water-borne and food-borne outbreaks of diarrheal diseases. PATIENTS AND METHODS: A total of 1685 fecal samples were collected from patients with gastrointestinal complaints who had been referred to clinical laboratories Tehran, Iran...
2016: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/27892657/effective-delivery-of-porphyrazine-photosensitizers-to-cancer-cells-by-polymer-brush-nanocontainers
#20
Natalia Y Shilyagina, Nina N Peskova, Svetlana A Lermontova, Anna A Brilkina, Vladimir A Vodeneev, Alexander V Yakimasky, Larisa G Klapshina, Irina V Balalaeva
Efficient drug delivery can be assigned to tasks that attract the most acute attention of researchers in the field of anticancer drug design. We have reported the first case of using amphiphilic polymer brushes as nanocontainers for photosensitizer delivery to cancer cells. Regular graft-copolymers of hydrophobic polyimides with hydrophilic polymethacrylic acid side chains were loaded with photosensitive dye tetra(4-fluorophenyl)tetracyanoporphyrazine (Pz) providing a sufficiently stable homogeneous fraction of fluorescent Pz-loaded nanoparticles with a size of 100-150 nm...
November 28, 2016: Journal of Biophotonics
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