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https://www.readbyqxmd.com/read/28212433/elevated-gm3-plasma-concentration-in-idiopathic-parkinson-s-disease-a-lipidomic-analysis
#1
Robin B Chan, Adler J Perotte, Bowen Zhou, Christopher Liong, Evan J Shorr, Karen S Marder, Un J Kang, Cheryl H Waters, Oren A Levy, Yimeng Xu, Hong Bin Shim, Itsik Pe'er, Gilbert Di Paolo, Roy N Alcalay
Parkinson's disease (PD) is a common neurodegenerative disease whose pathological hallmark is the accumulation of intracellular α-synuclein aggregates in Lewy bodies. Lipid metabolism dysregulation may play a significant role in PD pathogenesis; however, large plasma lipidomic studies in PD are lacking. In the current study, we analyzed the lipidomic profile of plasma obtained from 150 idiopathic PD patients and 100 controls, taken from the 'Spot' study at Columbia University Medical Center in New York. Our mass spectrometry based analytical panel consisted of 520 lipid species from 39 lipid subclasses including all major classes of glycerophospholipids, sphingolipids, glycerolipids and sterols...
2017: PloS One
https://www.readbyqxmd.com/read/28210753/puerarin-7-o-glucuronide-a-water-soluble-puerarin-metabolite-prevents-angiotensin-ii-induced-cardiomyocyte-hypertrophy-by-reducing-oxidative-stress
#2
Ning Hou, Bin Cai, Cai-Wen Ou, Zhen-Hui Zhang, Xia-Wen Liu, Mu Yuan, Gan-Jian Zhao, Shi-Ming Liu, Long-Gen Xiong, Jian-Dong Luo, Cheng-Feng Luo, Min-Sheng Chen
This study aimed to investigate the anti-oxidant and anti-hypertrophic effects of puerarin-7-O-glucuronide, a water-soluble puerarin metabolite. The anti-oxidant effects of puerarin-7-O-glucuronide were assessed by measurement of intracellular superoxide levels, total superoxide dismutase (SOD) activity, total anti-oxidant capacity, and glutathione (GSH)/glutathione disulfide (GSSG) ratio in cultured neonatal rat cardiomyocytes (NRCMs) stimulated with the xanthine oxidase (XO)/xanthine (X) system or angiotensin II...
February 16, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28208254/electrochemical-detection-of-escherichia-coli-from-aqueous-samples-using-engineered-phages
#3
Danhui Wang, Juhong Chen, Sam R Nugen
In this study, an enzyme-based electrochemical method was developed for the detection of Escherichia coli (E. coli) using the T7 bacteriophages engineered with lacZ operon encoding for beta-galactosidase (β-gal). The T7lacZ phages can infect E. coli, and have the ability to trigger the overexpression of β-gal during the infection of E. coli. The use of the engineered phages resulted in a more sensitive detection of E. coli by (1) overexpression of β-gal in E. coli during the specific infection and (2) release of the endogenous intracellular β-gal from E...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28205401/computer-aided-drug-design-to-explore-cyclodextrin-therapeutics-and-biomedical-applications
#4
Azizeh Abdolmaleki, Fatemeh Ghasemi, Jahan B Ghasemi
Cyclodextrin (CD) is a subset of the macrocyclic structural class, which is an important class of small organic agents that are useful functional excipients. They have wide range application possibilities in different fields of sciences such as material preparation, medicine, analytical chemistry, and separation processes. They are used widely in pharmaceutical formulations and drug delivery for increasing the water solubility of low soluble drugs and drug candidates. Due to the ring structure, they behave differently than smaller molecules and may be capable of hitting new classes of targets...
February 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28199328/association-of-pvuii-and-xbai-polymorphisms-on-estrogen-receptor-alpha-esr1-gene-to-changes-into-serum-lipid-profile-of-post-menopausal-women-effects-of-aging-body-mass-index-and-breast-cancer-incidence
#5
Neuza Felix Gomes-Rochette, Letícia Soncini Souza, Bruno Otoni Tommasi, Diego França Pedrosa, Sérgio Ragi Eis, Irani do Carmo Francischetto Fin, Fernando Luiz Herkenhoff Vieira, Jones Bernardes Graceli, Letícia Batista Azevedo Rangel, Ian Victor Silva
Estrogen is a steroidal hormone involved in several physiological functions in the female body including regulation of serum lipid metabolism and breast cancer (BC). Estrogen actions on serum lipids mostly occur through its binding to intracellular Estrogen Receptor alpha (ERalpha) isoform, expressed in most of tissues. This gene (ESR1) exhibit many polymorphic sites (SNPs) located either on translated and non-translated regions that regulate ERalpha protein expression and function. This paper aimed to investigate the association of two intronic SNPs of ESR1 gene, namely c454-397T>C (PvuII) and c454-351A>G (XbaI) to alterations in serum levels of total cholesterol (T-chol), total lipid (TL), low density lipoprotein cholesterol (LDL), high density lipoprotein (HDL), and triglycerides (TG) in a cohort of post-menopausal women...
2017: PloS One
https://www.readbyqxmd.com/read/28194980/cell-penetrating-peptide-spirolactam-derivative-as-a-reversible-fluorescent-ph-probe-for-live-cell-imaging
#6
Meng-Chan Xia, Lesi Cai, Sichun Zhang, Xinrong Zhang
A colorless and nonfluorescent spirolactam derivative, RhB-R12K, was synthesized by amide condensation between the carboxyl group of rhodamine B (RhB) and the amino group of cell-penetrating peptide (CPP). The fluorescence intensity of RhB-R12K sharply increased as the pH value decreased from 8.0 to 4.9, demonstrating sensitive and reversible response to intracellular pH distribution. This CPP probe was completely water soluble, had low cytotoxicity, was membrane permeable, and was suitable for pH measurement in various organelles by choosing organelle-specific CPP sequences...
January 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28193334/glutamate-dehydrogenase-rocg-in-bacillus-licheniformis-wx-02-enzymatic-properties-and-specific-functions-in-glutamic-acid-synthesis-for-poly-%C3%AE-glutamic-acid-production
#7
Guangming Tian, Qin Wang, Xuetuan Wei, Xin Ma, Shouwen Chen
Poly-γ-glutamic acid (γ-PGA), a natural biopolymer, is widely used in cosmetics, medicine, food, water treatment, and agriculture owing to its features of moisture sequestration, cation chelation, non-toxicity and biodegradability. Intracellular glutamic acid, the substrate of γ-PGA, is a limiting factor for high yield in γ-PGA production. Bacillus subtilis and Bacillus licheniformis are both important γ-PGA producing strains, and B. subtilis synthesizes glutamic acid in vivo using the unique GOGAT/GS pathway...
April 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28191619/hypothyroidism-reduces-mammary-tumor-progression-via-%C3%AE-catenin-activated-intrinsic-apoptotic-pathway-in-rats
#8
C M López Fontana, L E Zyla, F E Santiano, C V Sasso, F D Cuello-Carrión, V Pistone Creydt, M A Fanelli, R W Carón
Experimental hypothyroidism retards mammary carcinogenesis promoting apoptosis of tumor cells. β-catenin plays a critical role in cell adhesion and intracellular signaling pathways conditioning the prognosis of breast cancer. However, the mechanistic connections associated with the expression of β-catenin in thyroid status and breast cancer are not known. Therefore, we studied the relationship between the expression and localization of β-catenin and apoptosis in mammary tumors induced by 7,12-dimethylbenz(a)anthracene (DMBA) in hypothyroid (Hypot) and euthyroid (EUT) rats...
February 13, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28190443/neurologic-manifestations-of-major-electrolyte-abnormalities
#9
M Diringer
The brain operates in an extraordinarily intricate environment which demands precise regulation of electrolytes. Tight control over their concentrations and gradients across cellular compartments is essential and when these relationships are disturbed neurologic manifestations may develop. Perturbations of sodium are the electrolyte disturbances that most often lead to neurologic manifestations. Alterations in extracellular fluid sodium concentrations produce water shifts that lead to brain swelling or shrinkage...
2017: Handbook of Clinical Neurology
https://www.readbyqxmd.com/read/28188554/disinfection-by-product-formation-during-chlor-am-ination-of-algal-organic-matters-aom-extracted-from-microcystis-aeruginosa-effect-of-growth-phases-aom-and-bromide-concentration
#10
Juxiang Chen, Naiyun Gao, Lei Li, Mingqiu Zhu, Jing Yang, Xian Lu, Yansen Zhang
Algae organic matter (AOM), including extracellular organic matter (EOM) and intracellular organic matter (IOM), has caused a series of problems to the water quality, among which formation of disinfection by-products (DBPs) during subsequent chlor(am)ination process was especially serious and concerned. This study characterized physicochemical properties of the EOM and IOM solution extracted from different growth phases of Microcystis aeruginosa and investigated the corresponding formation potential of DBPs during chlor(am)ination process...
February 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28187768/current-lipid-extraction-methods-are-significantly-enhanced-adding-a-water-treatment-step-in-chlorella-protothecoides
#11
Xiaojie Ren, Xinhe Zhao, François Turcotte, Jean-Sébastien Deschênes, Réjean Tremblay, Mario Jolicoeur
BACKGROUND: Microalgae have the potential to rapidly accumulate lipids of high interest for the food, cosmetics, pharmaceutical and energy (e.g. biodiesel) industries. However, current lipid extraction methods show efficiency limitation and until now, extraction protocols have not been fully optimized for specific lipid compounds. The present study thus presents a novel lipid extraction method, consisting in the addition of a water treatment of biomass between the two-stage solvent extraction steps of current extraction methods...
February 11, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28182635/electrochemically-reduced-water-exerts-superior-reactive-oxygen-species-scavenging-activity-in-ht1080-cells-than-the-equivalent-level-of-hydrogen-dissolved-water
#12
Takeki Hamasaki, Gakuro Harada, Noboru Nakamichi, Shigeru Kabayama, Kiichiro Teruya, Bunshi Fugetsu, Wei Gong, Ichiro Sakata, Sanetaka Shirahata
Electrochemically reduced water (ERW) is produced near a cathode during electrolysis and exhibits an alkaline pH, contains richly dissolved hydrogen, and contains a small amount of platinum nanoparticles. ERW has reactive oxygen species (ROS)-scavenging activity and recent studies demonstrated that hydrogen-dissolved water exhibits ROS-scavenging activity. Thus, the antioxidative capacity of ERW is postulated to be dependent on the presence of hydrogen levels; however, there is no report verifying the role of dissolved hydrogen in ERW...
2017: PloS One
https://www.readbyqxmd.com/read/28179395/molecular-physiology-of-freeze-tolerance-in-vertebrates
#13
REVIEW
Kenneth B Storey, Janet M Storey
Freeze tolerance is an amazing winter survival strategy used by various amphibians and reptiles living in seasonally cold environments. These animals may spend weeks or months with up to ∼65% of their total body water frozen as extracellular ice and no physiological vital signs, and yet after thawing they return to normal life within a few hours. Two main principles of animal freeze tolerance have received much attention: the production of high concentrations of organic osmolytes (glucose, glycerol, urea among amphibians) that protect the intracellular environment, and the control of ice within the body (the first putative ice-binding protein in a frog was recently identified), but many other strategies of biochemical adaptation also contribute to freezing survival...
April 2017: Physiological Reviews
https://www.readbyqxmd.com/read/28177676/protective-effects-and-plausible-mechanisms-of-velvet-polypeptide-against-hydrogen-peroxide-induced-injury-in-human-umbilical-vein-endothelial-cells
#14
Wenhe Zhu, Huiyan Wang, Wei Zhang, Na Xu, Junjie Xu, Yan Li, Wensen Liu, Shijie Lv
Velvet antler polypeptide (VAP) is a prominent bioactive component of velvet antlers. Whereas, uncharacterized crude extracts were usually employed in pharmacological studies. In this study, the velvet polypeptide was isolated and purified by acid water extraction, ethanol precipitation, and ammonium sulfate fractionation and precipitation, and chromatography progressively. Human umbilical vein endothelial cells (HUVECs) were induced by H2O2 followed purified polypeptide treatment. Cell viability was evaluated by MTT assay...
January 4, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28171821/the-endoplasmic-reticulum-stress-inducer-thapsigargin-enhances-the-toxicity-of-zno-nanoparticles-to-macrophages-and-macrophage-endothelial-co-culture
#15
Gui Chen, Yuexin Shen, Xiyue Li, Qin Jiang, Shanshan Cheng, Yuxiu Gu, Liangliang Liu, Yi Cao
It was recently shown that exposure to ZnO nanoparticles (NPs) could induce endoplasmic reticulum (ER) stress both in vivo and in vitro, but the role of ER stress in ZnO NP induced toxicity remains unclear. Because macrophages are sensitive to ER stress, we hypothesized that stressing macrophages with ER stress inducer could enhance the toxicity of ZnO NPs. In this study, the effects of ER stress inducer thapsigargin (TG) on the toxicity of ZnO NPs to THP-1 macrophages were investigated. The results showed that TG enhanced ZnO NP induced cytotoxicity as revealed by water soluble tetrazolium-1 (WST-1) and neutral red uptake assays, but not lactate dehydrogenase (LDH) assay...
January 30, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28167409/activation-of-persulfates-by-natural-magnetic-pyrrhotite-for-water-disinfection-efficiency-mechanisms-and-stability
#16
Dehua Xia, Yan Li, Guocheng Huang, Ran Yin, Taicheng An, Guiying Li, Huijun Zhao, Anhuai Lu, Po Keung Wong
This study introduces natural occurring magnetic pyrrhotite (NP) as an environmentally friendly, easy available, and cost-effective alternative catalyst to activate persulfate (PS) of controlling microbial water contaminants. The E. coli K-12 inactivation kinetics observed in batch experiments was well described with first-order reaction. The optimum inactivation rate (k = 0.47 log/min) attained at a NP dose of 1 g/L and a PS dose of 1 mM, corresponding to total inactivation of 7 log10 cfu/mL cells within 15 min...
January 30, 2017: Water Research
https://www.readbyqxmd.com/read/28167259/memantine-reduces-the-production-of-amyloid-%C3%AE-peptides-through-modulation-of-amyloid-precursor-protein-trafficking
#17
Kaori Ito, Takuya Tatebe, Kunimichi Suzuki, Takashi Hirayama, Maki Hayakawa, Hideo Kubo, Taisuke Tomita, Mitsuhiro Makino
Memantine, an uncompetitive glutamatergic N-methyl-D-aspartate (NMDA) receptor antagonist, is widely used as medication for the treatment of Alzheimer's disease (AD). It has been reported that memantine reduces amyloid-β peptide (Aβ) levels in both neuronal cultures and in brains of animal models of AD. However, the underlying mechanism of these effects is unclear. Here we examined the effect of memantine on Aβ production. Memantine was administered to 9-month-old Tg2576 mice, a transgenic mouse model of AD, at 10 or 20mg/kg/day in drinking water for 1 month...
February 4, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28163959/kolaviron-a-biflavonoid-of-garcinia-kola-seed-mitigates-ischemic-reperfusion-injury-by-modulation-of-pro-survival-and-apoptotic-signaling-pathways
#18
Ademola Adetokunbo Oyagbemi, Dirk Bester, Johan Esterhuyse, Ebenezer Olatunde Farombi
OBJECTIVE: The study was designed to investigate the ameliorative effect of Kolaviron (KV) on ischemic/reperfusion injury in experimental animal models. MATERIALS AND METHODS: Male Wistar rats were randomly divided into two groups: Group 1 received corn oil as a vehicle and rats in Group 2 were administered KV at 200 mg/kg for 4 weeks. The rats were fed with rat standard chow pellet and water administered ad libitum. After 4 weeks of KV administration, hearts were excised and mounted on the working heart perfusion system...
January 2017: Journal of Intercultural Ethnopharmacology
https://www.readbyqxmd.com/read/28161549/a-new-targeted-delivery-approach-by-functionalizing-drug-nanocrystals-through-polydopamine-coating
#19
Honglei Zhan, Tina Jagtiani, Jun F Liang
Tumor target specificity via chemotherapy is widely considered to be very effective on tumor treatment. For an ideal chemotherapeutic agent like Camptothecin (CPT) CPT is the abbreviation for Camptothecin, improved therapeutic efficacy and high selectivity are equally important. Inspired by adhesive proteins in mussels, here we developed a novel tumor targeting peptide XQ1 grafted CPT nanocrystals with polydopamine coating as a spacer. In this study, CPT nanocrystals were coated by polymerization of dopamine that was induced by plasma-activated water under an acidic environment, and then the tumor targeting peptide was grafted onto polydopamine (PDA) PDA is the abbreviation for polydopamine coated CPT nanocrystals through catechol chemistry...
February 1, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28153588/positively-charged-phthalocyanine-arginine-conjugates-as-efficient-photosensitizer-for-photodynamic-therapy
#20
Ao Wang, Rongrong Zhou, Lin Zhou, Kang Sun, Jianchun Jiang, Shaohua Wei
Positively charged drugs usually have enhanced water solubility, cellular uptake efficiency and anticancer activity. However, the common quaternized and protonated cationic photosensitizers both have some drawbacks such as needing potentially dangerous agents for preparation and easily being deprotonated in alkaline circumstance. Arginine is unique among the amino acids as its guanidine group has exceptionally high basicity in aqueous solution, which may make it positively charged in a wide range of pH. In this paper, two arginine substituted zinc phthalocyanines (ArgEZnPc and ArgZnPc) were reported...
January 21, 2017: Bioorganic & Medicinal Chemistry
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