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Adipose mesenchymal stem cells conditioned media

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https://www.readbyqxmd.com/read/29684675/fak-and-yap-taz-dependent-mechanotransduction-pathways-are-required-for-enhanced-immunomodulatory-properties-of-adipose-derived-mesenchymal-stem-cells-induced-by-aligned-fibrous-scaffolds
#1
S Wan, X Fu, Y Ji, M Li, X Shi, Y Wang
Adipose-derived mesenchymal stem cells (ASCs) represent an excellent candidate for tissue engineering due to their multipotency and paracrine functions. Physical cues provided by the scaffolds have been shown to direct a variety of cellular behaviors of ASCs; however, their effects on the paracrine functions of ASCs, especially immunomodulatory functions, remain largely unexplored. Thus, PLLA electrospun fibrous scaffolds, with fibers oriented either randomly or aligned, were used as a model to investigate the effects of fiber orientation on the immunomodulatory paracrine secretion of ASCs...
April 16, 2018: Biomaterials
https://www.readbyqxmd.com/read/29506631/the-osmolyte-type-affects-cartilage-associated-pathologic-marker-expression-during-in-vitro-mesenchymal-stem-cell-chondrogenesis-under-hypertonic-conditions
#2
Sorour Ahmadyan, Mahboubeh Kabiri, Noushin Tasharofi, Simzar Hosseinzadeh, Mousa Kehtari, Athena Hajari Zadeh, Masoud Soleimani, Ali Farazmand, Hana Hanaee-Ahvaz
Stem cells' fate during in vitro differentiation is influenced by biophysicochemical cues. Osmotic stress has proved to enhance chondrocyte marker expression, however its potent negative impacts had never been surveyed. We questioned whether specific osmotic conditions, regarding the osmolyte agent, could benefit chondrogenesis while dampening undesired concomitant hypertrophy and inflammatory responses. To examine the potential side effects of hypertonicity, we assessed cell proliferation as well as chondrogenic and hypertrophic marker expression of human Adipose Derived-MSC after a two week induction in chondrogenic media with either NaCl or Sorbitol, as the osmolyte agent to reach a +100 mOsm hypertonic condition...
February 28, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29489829/trophic-effects-of-adipose-tissue-derived-and-bone-marrow-derived-mesenchymal-stem-cells-enhance-cartilage-generation-by-chondrocytes-in-co-culture
#3
M M Pleumeekers, L Nimeskern, J L M Koevoet, M Karperien, K S Stok, G J V M van Osch
AIMS: Combining mesenchymal stem cells (MSCs) and chondrocytes has great potential for cell-based cartilage repair. However, there is much debate regarding the mechanisms behind this concept. We aimed to clarify the mechanisms that lead to chondrogenesis (chondrocyte driven MSC-differentiation versus MSC driven chondroinduction) and whether their effect was dependent on MSC-origin. Therefore, chondrogenesis of human adipose-tissue-derived MSCs (hAMSCs) and bone-marrow-derived MSCs (hBMSCs) combined with bovine articular chondrocytes (bACs) was compared...
2018: PloS One
https://www.readbyqxmd.com/read/29445958/histopathological-and-behavioral-assessments-of-aging-effects-on-stem-cell-transplants-in-an-experimental-traumatic-brain-injury
#4
Jea-Young Lee, Roger Lin, Hung Nguyen, M Grant Liska, Trenton Lippert, Yuji Kaneko, Cesar V Borlongan
Traumatic brain injury (TBI) displays cognitive and motor symptoms following the initial injury which can be exacerbated by secondary cell death. Aging contributes significantly to the morbidity of TBI, with higher rates of negative neurological and behaviors outcomes. In the recent study, young and aged animals were injected intravenously with human adipose-derived mesenchymal stem cells (hADSCs) (Tx), conditioned media (CM), or vehicle (unconditioned media) following TBI. The beneficial effects of hADSCs were analyzed using various molecular and behavioral techniques...
February 15, 2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29434773/human-adipose-derived-mesenchymal-stem-cell-conditioned-media-suppresses-inflammatory-bone-loss-in-a-lipopolysaccharide-induced-murine-model
#5
Yu Li, Xin Gao, Jinbing Wang
Conditioned media (CM) from mesenchymal stem cells (MSCs) contains various cytokines, growth factors and microRNAs, which may serve important roles in modulating the inflammatory process. However, the effect of MSC-CM on inflammatory bone loss remains unknown. The present study investigated the effects of conditioned media from human adipose-derived mesenchymal stem cells (AMSC-CM) on the prevention of lipopolysaccharide (LPS)-mediated bone loss in mice. To investigate the underlying mechanisms of this effect, the effects of AMSC-CM on serum levels of inflammation-associated cytokines [tumor necrosis factor (TNF)-α, interleukin (IL)-1, IL-6 and IL-10] in LPS-treated mice, in addition to their mRNA expression in LPS-treated macrophages, was investigated...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29429509/physical-stimulation-and-scaffold-composition-efficiently-support-osteogenic-differentiation-of-mesenchymal-stem-cells
#6
Sepide Heydari Asl, Hoorieh Hosseinpoor, Kazem Parivar, Nasim Hayati Roodbari, Hana Hanaee-Ahvaz
BACKGROUND: Despite significant achievements in the field of tissue engineering, simplification and improvement of the existing protocols are of great importance. The use of complex differentiation media, due to the presence of multiple factors, may have some undesired effects on cell health and functions. Thus, minimizing the number of involved factors, while maintaining the differentiation efficiency, provides less costly and controllable conditions. Adipose-derived Mesenchymal stem cells (ASCs), the adult stem cells present in adipose tissue, can be a suitable source of stem cells due to abundant and ease of access...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29384015/the-neuroprotective-effect-of-rat-adipose-tissue-derived-mesenchymal-stem-cell-conditioned-medium-on-cortical-neurons-using-an-in-vitro-model-of-sci-inflammation
#7
Eva Szekiova, Lucia Slovinska, Juraj Blasko, Jana Plsikova, Dasa Cizkova
Objectives In this study, a new approach was used with an in vitro model in which neural cells were exposed to conditioned media from the injured spinal cord (SCI-CM) mimicking a local inflammatory microenvironment . Subsequently, the neuroprotective effect of rat adipose tissue-derived msesenchymal stem cell-conditioned media (ATMSC-CM) was investigated through a cell-free based therapy, which was used to treat cortical neurons and astrocytes under inflammation. Methods Primary cell cultures isolated from postnatal day (P6) Wistar rat brain cortex were exposed to SCI-CM derived from the central lesion, rostral and caudal segments of injured spinal cord...
January 31, 2018: Neurological Research
https://www.readbyqxmd.com/read/29198672/hypoxia-induced-damage-in-human-islets-is-reduced-with-the-use-of-mesenchymal-stem-cell-preconditioned-medium
#8
D Brandhorst, H Brandhorst, S Acreman, S W Schive, H Bjørnson Scholz, P R V Johnson
BACKGROUND: Mesenchymal stem cells (MSCs) are protective for islets when cotransplanted in a hypoxic environment. However, the risk of neoplasia is increased when MSCs are transplanted into immunosuppressed patients. This initial study aimed to investigate whether the production of protective factors from MSC can be stimulated by different culture conditions to benefit human islets cultured in hypoxia. METHODS: MSC were isolated from human adipose tissue and cultured for 2 days in supplemented Minimum Essential Media α (MEMα) and 21% (21%-MEMα) or 1% oxygen (1%-MEMα)...
December 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29076586/cancer-and-normal-adipose-derived-mesenchymal-stem-cells-ascs-do-they-have-differential-effects-on-tumor-and-immune-cells
#9
Mahboobeh Razmkhah, Zahra Mansourabadi, Maryam Sadat Mohtasebi, Abdol-Rasoul Talei, Abbas Ghaderi
Adipose-derived mesenchymal stem cells (ASCs) are known to have immunomodulatory properties through soluble factors or by direct cell-to-cell contact. This study aimed to assess the expression of HLA-G and IDO activity in breast cancer and normal ASCs and to see whether ASC is capable of modulating both tumor cells and immune system cells in vitro. ASCs were enzymatically isolated from 15 breast cancer patients and 10 normal individuals. Then they were cultured, and the impact of their conditioned media on the movement of the MDA-MB-231 breast cancer cell line was studied in wound healing scratch assay...
March 2018: Cell Biology International
https://www.readbyqxmd.com/read/28965958/secretome-released-from-hydrogel-embedded-adipose-mesenchymal-stem-cells-protects-against-the-parkinson-s-disease-related-toxin-6-hydroxydopamine
#10
Armando Chierchia, Nino Chirico, Lucia Boeri, Ilaria Raimondi, Giovanni A Riva, Manuela Teresa Raimondi, Marta Tunesi, Carmen Giordano, Gianluigi Forloni, Diego Albani
Neurodegenerative diseases, as Parkinson's disease (PD), involve irreversible neural cell damage and impairment. In PD, there is a selective degeneration of the dopaminergic neurons leading to motor symptoms. A common finding in PD neurodegeneration is the increase of reactive oxygen species (ROS), leading to oxidative stress. To date there are only interventions to relieve PD symptoms, however progress has been made in the development of therapies that target the immune system or use its components as therapeutic agents; among these, mesenchymal stem cells (MSCs), which are able to express neuroprotective factors as cytokines, chemokines and angiogenic molecules, collectively named secretome, that accumulate in MSC culture medium...
December 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28875863/the-basic-mechanism-of-hair-growth-stimulation-by-adipose-derived-stem-cells-and-their-secretory-factors
#11
Chong Hyun Won, Gyeong-Hun Park, Xunwei Wu, Thanh-Nga Tran, Ki-Young Park, Byung-Soon Park, Dong Young Kim, Ohsang Kwon, Kyu-Han Kim
BACKGROUND: Adipose-derived stem cells (ADSCs) are mesenchymal stem cells (MSCs) within the stromal vascular fraction of subcutaneous adipose tissue. ADSCs secrete growth factors and other proteins, and have been used to regenerate skin with satisfactory results. OBJECTIVE: This review focuses on the effect of ADSCs and their secretory factors on the stimulation of hair growth in vitro, ex vivo and in vivo. RESULTS: The conditioned media of ADSCs (ADSC-CM) increases the proliferation rate of human follicular cells...
2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28841158/mesenchymal-stem-cell-secretome-toward-cell-free-therapeutic-strategies-in-regenerative-medicine
#12
REVIEW
Francisco J Vizoso, Noemi Eiro, Sandra Cid, Jose Schneider, Roman Perez-Fernandez
Earlier research primarily attributed the effects of mesenchymal stem cell (MSC) therapies to their capacity for local engrafting and differentiating into multiple tissue types. However, recent studies have revealed that implanted cells do not survive for long, and that the benefits of MSC therapy could be due to the vast array of bioactive factors they produce, which play an important role in the regulation of key biologic processes. Secretome derivatives, such as conditioned media or exosomes, may present considerable advantages over cells for manufacturing, storage, handling, product shelf life and their potential as a ready-to-go biologic product...
August 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28836202/isolation-and-culture-of-adipose-derived-stromal-cells-from-subcutaneous-fat
#13
Lawrence J Liew, Huan Ting Ong, Rodney J Dilley
Cells with mesenchymal stem cell characteristics can be isolated from human adipose tissue stroma. Relative ease of isolation in large numbers and their ability for expansion and differentiation means that they are becoming a preferred cell type for mesenchymal regenerative medicine applications. In addition to expansion and differentiation capacity, they also express valuable paracrine activities which promote tissue formation and wound healing, including pro- and anti-fibrotic mediators. Here we describe a method for routine isolation of adipose stromal cells, culture expansion, and characterization by differentiation and then production of conditioned media...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28713640/human-adipose-derived-mesenchymal-stem-cells-respond-to-short-term-hypoxia-by-secreting-factors-beneficial-for-human-islets-in-vitro-and-potentiate-antidiabetic-effect-in-vivo
#14
Simen W Schive, Mohammad Reza Mirlashari, Grete Hasvold, Mengyu Wang, Dag Josefsen, Hans Petter Gullestad, Olle Korsgren, Aksel Foss, Gunnar Kvalheim, Hanne Scholz
Adipose-derived mesenchymal stem cells (ASCs) release factors beneficial for islets in vitro and protect against hyperglycemia in rodent models of diabetes. Oxygen tension has been shown to induce metabolic changes and alter ASCs' release of soluble factors. The effects of hypoxia on the antidiabetic properties of ASCs have not been explored. To investigate this, we incubated human ASCs for 48 h in 21% (normoxia) or 1% O2 (hypoxia) and compared viability, cell growth, surface markers, differentiation capability, and soluble factors in the conditioned media (CM)...
2017: Cell Medicine
https://www.readbyqxmd.com/read/28695799/effect-of-human-hepatocellular-carcinoma-hepg2-cell-derived-exosome-on-the-differentiation-of-mesenchymal-stem-cells-and-their-interaction
#15
Fei Luo, Zhao Sun, Qin Han, Chunling Xue, Chunmei Bai
Objective To investigate the effect of human hepatocellular carcinoma HepG2 cell-derived Exosome on the differentiation of mesenchymal stem cells(MSC)into cancer-associated myofibroblasts(CAF)and the impacts of CAF on liver cancer cell proliferation,migration,and invasion. Methods The protein expression of HepG2 cell-derived Exosome was detected by Western blotting. MSCs were separated from human adipose tissue and cultured with HepG2 cell-derived Exosome(100 ng/nl)to initiate differentiation. The expressions of mesenchymal markers and several interleukins were also detected by Western blotting...
June 20, 2017: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/28648118/physiological-effects-of-proinsulin-connecting-peptide-in-human-subcutaneous-adipose-tissue
#16
A Ghorbani, R Shafiee-Nick, S A Zojaji, M T Rajabi-Mashhadi
Recent studies suggest that proinsulin-connecting peptide (C-peptide) may exhibit characteristics of a hormone and show physiological functions in various tissues. This study was aimed to determine whether C-peptide could be involved in the regulation of lipolysis, adiponectin release, and function of mesenchymal stem cells (MSCs) in adipose tissue. Human subcutaneous adipose tissue was cultured in the presence of C-peptide. The level of lipolysis was determined by glycerol measurement in the conditioned media...
June 1, 2017: Physiology International
https://www.readbyqxmd.com/read/28628602/procoagulant-activity-of-human-mesenchymal-stem-cells
#17
Barbara A Christy, Maryanne C Herzig, Robbie K Montgomery, Christopher Delavan, James A Bynum, Kristin M Reddoch, Andrew P Cap
BACKGROUND: Allogeneic mesenchymal stem cells (MSCs) show great potential for the treatment of military and civilian trauma based on their reduced immunogenicity and ability to modulate inflammation and immune function in the recipient. Although generally considered to be safe, MSCs express tissue factor (TF), a potent activator of coagulation. In the current study, we evaluated multiple MSC populations for tissue factor expression and procoagulant activity to characterize safety considerations for systemic use of MSCs in trauma patients who may have altered coagulation homeostasis...
July 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28620864/adipose-tissue-derived-stromal-cells-adsc-express-oligodendrocyte-and-myelin-markers-but-they-do-not-function-as-oligodendrocytes
#18
Lara Vellosillo, Maria Paz Muñoz, Carlos Luis Paíno
Mesenchymal cells cultured from the vasculo-stromal fraction of adipose tissue (ADSC) show adult stem cell characteristics and several groups have claimed generating neural cells from them. However, we have observed that many markers commonly used for the identification of neural cells are spontaneously expressed by ADSC in culture. In the present study, we have examined the expression of characteristic oligodendrocyte molecules in cultured ADSC, aiming to test if myelinating cells could be generated from accessible non-neural adult tissues...
November 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28618870/molecular-validation-of-chondrogenic-differentiation-and-hypoxia-responsiveness-of-platelet-lysate-expanded-adipose-tissue-derived-human-mesenchymal-stromal-cells
#19
Catalina Galeano-Garces, Emily T Camilleri, Scott M Riester, Amel Dudakovic, Dirk R Larson, Wenchun Qu, Jay Smith, Allan B Dietz, Hee-Jeong Im, Aaron J Krych, A Noelle Larson, Marcel Karperien, Andre J van Wijnen
OBJECTIVE: To determine the optimal environmental conditions for chondrogenic differentiation of human adipose tissue-derived mesenchymal stromal/stem cells (AMSCs). In this investigation we specifically investigate the role of oxygen tension and 3-dimensional (3D) culture systems. DESIGN: Both AMSCs and primary human chondrocytes were cultured for 21 days in chondrogenic media under normoxic (21% oxygen) or hypoxic (2% oxygen) conditions using 2 distinct 3D culture methods (high-density pellets and poly-ε-caprolactone [PCL] scaffolds)...
July 2017: Cartilage
https://www.readbyqxmd.com/read/28533959/preservation-media-durations-and-cell-concentrations-of-short-term-storage-affect-key-features-of-human-adipose-derived-mesenchymal-stem-cells-for-therapeutic-application
#20
Fengli Zhang, Huaijuan Ren, Xiaohu Shao, Chao Zhuang, Yantian Chen, Nianmin Qi
BACKGROUND: Adipose-derived mesenchymal stem cells (ADSCs) have shown great potential in the treatment of various diseases. However, the optimum short-term storage condition of ADSCs in 2∼8 °C is rarely reported. This study aimed at optimizing a short-term storage condition to ensure the viability and function of ADSCs before transplantation. METHODS: Preservation media and durations of storage were evaluated by cell viability, apoptosis, adhesion ability and colony-forming unit (CFU) capacity of ADSCs...
2017: PeerJ
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