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Adipose mesenchymal stem cells conditioned media

Amanda Faria Assoni, Giuliana Castello, Marcos Valadares, Melinda Beccari, Juliana Gomes, Mayra Pelatti, Miguel Mitne-Neto, Valdemir Melechco Carvalho, Mayana Zatz
Duchenne muscular dystrophy (DMD) is a lethal X-linked disorder caused by null mutations in the dystrophin gene. Although the primary defect is the deficiency of muscle dystrophin, secondary events, including chronic inflammation, fibrosis and muscle regeneration failure are thought to actively contribute to disease progression. Despite several advances, there is still no effective therapy for DMD. Therefore, the potential regenerative capacities, as well as immune-privileged properties of Mesenchymal Stromal Cells (MSCs), have been the focus of intense investigation in different animal models aiming the treatment of these disorders...
October 20, 2016: Stem Cells and Development
Serban San-Marina, Ayushman Sharma, Stephen G Voss, Jeffrey R Janus, Grant S Hamilton
Importance: Nasal reconstruction in patients who are missing a significant amount of structural nasal support remains a difficult challenge. One challenge is the deficiency of cartilage left within the nose as a consequence of rhinectomy or a midline destructive disease. Historically, the standard donor source for large quantities of native cartilage has been costal cartilage. Objective: To enable the development of protocols for new mesenchymal stem cell technologies as alternative procedures with reduced donor site morbidity, risk of infection and extrusion...
October 13, 2016: JAMA Facial Plastic Surgery
Ahmed Saad, Xiang-Yang Zhu, Sandra Herrmann, LaTonya Hickson, Hui Tang, Allan B Dietz, Andre J van Wijnen, Lilach Lerman, Stephen Textor
BACKGROUND: Adipose-derived MSC (AMSCs) possess angiogenic and immunomodulatory properties that may modulate kidney regeneration. Whether these properties are retained in older patients with atherosclerotic vascular disease is poorly understood. Hypoxic conditions are known to modify properties and growth characteristics of AMSCs. We tested the hypothesis that AMSCs from older patients with atherosclerotic renovascular disease (RVD) differ from normal kidney donors, and whether hypoxia changes their functional and molecular properties to promote angiogenesis...
2016: Stem Cell Research & Therapy
Arash Valadbeygi, Tahere Naji, Afshin Pirnia, Mohammadreza Gholami
Successful freezed-thaw of adipose-derived mesenchymal stem cells (ADMSCs) could be a major step in regenerative medicine as well as in the cloning of animal breeds. The aim of this study was to evaluate the efficacy of selenium on the optimizing of freezed-thaw media in the ADMSCs. ADMSCs were extracted from NMRI mice and purified with positive selection Monoclonal CD105 Antibody (PE) and negative selection Monoclonal CD31 and CD45 Antibody using MACS method as well as differentiation to adipose and bone tissue...
October 2016: Cryobiology
Moysés S Miranda, Hamilton S Nascimento, Mayra P R Costa, Nathália N Costa, Karynne N L Brito, Cinthia T A Lopes, Simone S D Santos, Marcela S Cordeiro, Otávio M Ohashi
PURPOSE: Despite advances in the composition of defined embryo culture media, co-culture with somatic cells is still used for bovine in vitro embryo production (IVEP) in many laboratories worldwide. Granulosa cells are most often used for this purpose, although recent work suggests that co-culture with stem cells of adult or embryonic origin or their derived biomaterials may improve mouse, cattle, and pig embryo development. MATERIALS AND METHODS: In experiment 1, in vitro produced bovine embryos were co-cultured in the presence of two concentrations of bovine adipose tissue-derived mesenchymal cells (b-ATMSCs; 10(3) and 10(4) cells/mL), in b-ATMSC preconditioned medium (SOF-Cond), or SOF alone (control)...
October 2016: Journal of Assisted Reproduction and Genetics
Hussein Sheashaa, Ahmed Lotfy, Fatma Elhusseini, Azza Abdel Aziz, Azza Baiomy, Samah Awad, Aziza Alsayed, Abdel-Hady El-Gilany, Mohamed-Ahdy A A Saad, Khaled Mahmoud, Faten Zahran, Dalia A Salem, Ahmed Sarhan, Hassan Abdel Ghaffar, Mohamed Sobh
Acute kidney injury (AKI) is a complex clinical condition associated with significant morbidity and mortality and lacking effective management. Ischemia-reperfusion injury (IRI) remains one of the leading causes of AKI in native and transplanted kidneys. The aim of this study was to evaluate the efficacy of adipose-derived mesenchymal stem cells (ADSCs) in the prevention of renal IRI in rats. The study was conducted on male Sprague-Dawley rats (n=72) weighing 250-300 g. Rats were randomly assigned to three main groups: i) Sham-operated control group (n=24); ii) positive control group, in which rats were subjected to IRI and were administered culture media following 4 h of IRI (n=24); and iii) ADSC group (n=24), in which rats were administered 1×10(6) ADSCs via the tail vein following 4 h of IRI...
May 2016: Experimental and Therapeutic Medicine
D W Weisgerber, K Erning, C L Flanagan, S J Hollister, B A C Harley
A particular challenge in biomaterial development for treating orthopedic injuries stems from the need to balance bioactive design criteria with the mechanical and geometric constraints governed by the physiological wound environment. Such trade-offs are of particular importance in large craniofacial bone defects which arise from both acute trauma and chronic conditions. Ongoing efforts in our laboratory have demonstrated a mineralized collagen biomaterial that can promote human mesenchymal stem cell osteogenesis in the absence of osteogenic media but that possesses suboptimal mechanical properties in regards to use in loaded wound sites...
August 2016: Journal of the Mechanical Behavior of Biomedical Materials
A Carbone, M Valente, L Annacontini, S Castellani, S Di Gioia, D Parisi, M Rucci, G Belgiovine, C Colombo, A Di Benedetto, G Mori, L Lo Muzio, A Maiorella, A Portincasa, M Conese
The potential of adipose-derived mesenchymal stromal (stem) cells (ADSCs) to differentiate into either osteoblasts or chondrocytes is controversial. In this study we investigated the multicapacity potential of ADSCs to differentiate towards adipocyte, osteoblast, and chondrocyte lineages when cells are seeded onto plastic in comparison with incubation with conditioned media (CM) obtained from differentiated cell types.ADSCs, obtained from liposuctions, were characterized for mesenchymal and hematopoietic markers by cytofluorimetry...
January 2016: Journal of Biological Regulators and Homeostatic Agents
Annika Weigand, Anja M Boos, Kereshmeh Tasbihi, Justus P Beier, Paul D Dalton, Michael Schrauder, Raymund E Horch, Matthias W Beckmann, Pamela L Strissel, Reiner Strick
BACKGROUND: There is a need to establish more cell lines from breast tumors in contrast to immortalized cell lines from metastatic effusions in order to represent the primary tumor and not principally metastatic biology of breast cancer. This investigation describes the simultaneous isolation, characterization, growth and function of primary mammary epithelial cells (MEC), mesenchymal cells (MES) and adipose derived stem cells (ADSC) from four normal breasts, one inflammatory and one triple-negative ductal breast tumors...
2016: Breast Cancer Research: BCR
Forouzan Yousefi, Massoumeh Ebtekar, Sara Soudi, Masoud Soleimani, Seyed Mahmoud Hashemi
Mesenchymal stem cells (MSCs) are well known to possess neuroprotective and immunomodulatory effects, due to cell-to-cell interaction and their soluble factors. We conducted a comparative analysis of the immunomodulatory properties of adipose tissue mesenchymal stem cells (AT-MSCs) and their conditioned media (CM), derived from C57/BL6 mice, for mitigating the adverse clinical course of experimental autoimmune encephalomyelitis (EAE). We measure IL4, IL17 and IFNɣ production of supernatant from spleen cells...
April 2016: Immunology Letters
Carlos Hugo Escobar, Orlando Chaparro
Molecules of animal or bacterial origin, which pose a risk for zoonoses or immune rejection, are commonly used for extraction, culture, and cryopreservation of mesenchymal stem cells. There is no sequential and orderly protocol for producing human adipose-derived stem cells (hASCs) under xeno-free conditions. After standardizing a human platelet lysate (hPL) production protocol, four human adipose tissue samples were processed through explants with fetal bovine serum (FBS)-supplemented or hPL-supplemented media for extracting the adipose-derived stem cells...
March 2016: Stem Cells Translational Medicine
Drenka Trivanović, Aleksandra Jauković, Jelena Krstić, Srdjan Nikolić, Ivana Okić Djordjević, Tamara Kukolj, Hristina Obradović, Slavko Mojsilović, Vesna Ilić, Juan Francisco Santibanez, Diana Bugarski
Mesenchymal stem cells from human adipose tissue (hASCs) are proposed as suitable tools for soft tissue engineering and reconstruction. Although it is known that hASCs have the ability to home to sites of inflammation and tumor niche, the role of inflammatory cytokines in the hASCs-affected tumor development is not understood. We found that interferon-γ (IFN-γ) and/or tumor necrosis factor-α (TNF-α) prime hASCs to produce soluble factors which enhance MCF-7 cell line malignancy in vitro. IFN-γ and/or TNF-α-primed hASCs produced conditioned media (CM) which induced epithelial to mesenchymal transition (EMT) of MCF-7 cells by reducing E-Cadherin and increasing Vimentin expression...
March 2016: IUBMB Life
Cristina Castells-Sala, Lourdes Recha-Sancho, Aida Llucià-Valldeperas, Carolina Soler-Botija, Antoni Bayes-Genis, Carlos E Semino
The prolonged ischemia after myocardial infarction leads to a high degree of cardiomyocyte death, which leads to a reduction of normal heart function. Valuable lessons can be learnt from human myocardium and stem cell biology that would help scientists to develop new, effective, safe, and affordable regenerative therapies. In vivo models are of high interest, but their high complexity limits the possibility to analyze specific factors. In vitro models permit analyzing specific factors of tissue physiology or pathophysiology providing accurate approaches that may guide the creation of three-dimensional (3D) engineered cell aggregates...
January 7, 2016: Tissue Engineering. Part C, Methods
Wan Kamarul Zaman Wan Safwani, Chin Wei Wong, Kar Wey Yong, Jane Ru Choi, Noor Azmi Mat Adenan, Siti Zawiah Omar, Wan Abu Bakar Wan Abas, Belinda Pingguan-Murphy
The need to have a better and safer culture condition for expansion of human mesenchymal stem cells (MSCs) is crucial particularly to prevent infection and immune rejection. This is normally associated with the use of animal-based serum in the culture media for cell expansion. The aim of this study is to investigate alternative culture conditions which may provide better and safer environment for cell growth. In the present study, human adipose-derived stem cells (ASCs) at passage 3 were subjected to treatment in 4 conditions: (1) 21 % O2 with fetal bovine serum (FBS), (2) 21 % O2 without FBS, (3) 2 % O2 with FBS and (4) 2 % O2 without FBS followed by subsequent analysis of their phenotype, viability and functionality...
October 2016: Cytotechnology
G D Sagaradze, O A Grigorieva, A Yu Efimenko, A A Chaplenko, S N Suslina, V Yu Sysoeva, N I Kalinina, Zh A Akopyan, V A Tkachuk
Regenerative medicine approaches, such as replacement of damaged tissue by ex vivo manufactured constructions or stimulation of endogenous reparative and regenerative processes to treat different diseases, are actively developing. One of the major tools for regenerative medicine are stem and progenitor cells, including multipotent mesenchymal stem/stromal cells (MSC). Because the paracrine action of bioactive factors secreted by MSC is considered as a main mechanism underlying MSC regenerative effects, application of MSC extracellular secreted products could be a promising approach to stimulate tissue regeneration; it also has some advantages compared to the injection of the cells themselves...
November 2015: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
Kaitlin C Clark, Amir Kol, Salpi Shahbenderian, Jennifer L Granick, Naomi J Walker, Dori L Borjesson
Mesenchymal stem cell (MSC) therapy is being increasingly used to treat dogs and horses with naturally-occurring diseases. However these animals also serve as critical large animal models for ongoing translation of cell therapy products to the human market. MSC manufacture for clinical use mandates improvement in cell culture systems to meet demands for higher MSC numbers and removal of xeno-proteins (i.e. fetal bovine serum, FBS). While serum-free media (SFM) is commercially available, its affects on MSC phenotype and immunomodulatory functions are not fully known...
April 2016: Stem Cell Reviews
Joseph J Shearer, Eric A Wold, Charles S Umbaugh, Cheryl F Lichti, Carol L Nilsson, Marxa L Figueiredo
BACKGROUND: The tumor microenvironment plays an important role in the progression of cancer by mediating stromal-epithelial paracrine signaling, which can aberrantly modulate cellular proliferation and tumorigenesis. Exposure to environmental toxicants, such as inorganic arsenic (iAs), has also been implicated in the progression of prostate cancer. OBJECTIVE: The role of iAs exposure in stromal signaling in the tumor microenvironment has been largely unexplored...
July 2016: Environmental Health Perspectives
Camilla Siciliano, Isotta Chimenti, Mohsen Ibrahim, Chiara Napoletano, Giorgio Mangino, Gaia Scafetta, Giuseppe Biondi Zoccai, Erino Angelo Rendina, Antonella Calogero, Giacomo Frati, Elena De Falco
Nowadays, cardiac regenerative medicine is facing many limitations because of the complexity to find the most suitable stem cell source and to understand the regenerative mechanisms involved. Mesenchymal stem cells (MSCs) have shown great regenerative potential due to their intrinsic properties and ability to restore cardiac functionality, directly by transdifferentiation and indirectly by paracrine effects. Yet, how MSCs could respond to definite cardiac-committing microenvironments, such as that created by resident cardiac progenitor cells in the form of cardiospheres (CSs), has never been addressed...
2015: Cell Transplantation
Yanju Li, Xiao Xu, Lihua Wang, Guangjin Liu, Yanqi Li, Xiaobing Wu, Yongguang Jing, Haiyan Li, Guihua Wang
BACKGROUND: Cellular senescence is linked to aging and tumorigenesis. The senescence of mesenchymal stem cells (MSCs) may influence the tumor growth, metastasis, and angiogenesis by secreting a variety of cytokines and growth factors. RESULTS: The conditioned media of adipose derived MSCs (AD-MSCs) stimulated the proliferation of human LoVo colorectal-cancer cells, and the replicative senescent MSCs had the more obvious effects in comparison to that of premature AD-MSCs...
2015: Cell & Bioscience
Xin Li, Peng Zhang, Xiaozhi Liu, Peng Lv
BACKGROUND: Studies have revealed mesenchymal cells tend to directionally migrate toward tumor cells and inhibit tumor growth. However, there have been rare reports about adipose-derived mesenchymal stem cells (AMSCs), which achieved stable expression of interleukin (IL)-12 to inhibit lung adenocarcinoma cell migration and invasion. We aimed to achieve stable expression of IL-12 in AMSCs through transgenic technology and utilize the paracrine effect of IL-12 to inhibit lung adenocarcinoma cell migration and invasion...
January 2015: Thoracic Cancer
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